MS016821 (gene) Bitter gourd (TR) v1

BLAST of MS016821 vs. NCBI nr
Match: XP_022144338.1 (S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase [Momordica charantia])

HSP 1 Score: 1300.0 bits (3363), Expect = 0.0e+00
Identity = 638/639 (99.84%), Postives = 638/639 (99.84%), Query Frame = 0

Query: 1   MSMSSLSARLTLLALLSASTFYFFYKSRRLRKLKLSLNPTVCSRRAKLFFISETGTSKAL 60
           MSMSSLSARLTLLALLSASTFYFFYKSRRLRKLKLSLNPTVCSRRAKLFFISETGTSKAL
Sbjct: 1   MSMSSLSARLTLLALLSASTFYFFYKSRRLRKLKLSLNPTVCSRRAKLFFISETGTSKAL 60

Query: 61  AHRLLNVLNSNELAFDLVDPEDYEPEDLFKESLVLFVASTWEDGGPPPHAKFFANWLSES 120
           AHRLLNVLNSNELAFDLVDPEDYEPEDLFKESLVL VASTWEDGGPPPHAKFFANWLSES
Sbjct: 61  AHRLLNVLNSNELAFDLVDPEDYEPEDLFKESLVLLVASTWEDGGPPPHAKFFANWLSES 120

Query: 121 AEDFRVGSLLLSECKFGVFGVGSRAYGDTFNAVARGFSKQMRALGAKEILPVGEGDVDGG 180
           AEDFRVGSLLLSECKFGVFGVGSRAYGDTFNAVARGFSKQMRALGAKEILPVGEGDVDGG
Sbjct: 121 AEDFRVGSLLLSECKFGVFGVGSRAYGDTFNAVARGFSKQMRALGAKEILPVGEGDVDGG 180

Query: 181 DIDKCFDDWSQQVLKVLKVDAVGSGTDLYSAIAGDSDAESVEDDEEDADLGAEDIVDLED 240
           DIDKCFDDWSQQVLKVLKVDAVGSGTDLYSAIAGDSDAESVEDDEEDADLGAEDIVDLED
Sbjct: 181 DIDKCFDDWSQQVLKVLKVDAVGSGTDLYSAIAGDSDAESVEDDEEDADLGAEDIVDLED 240

Query: 241 IAGKAPSRKSTNVVETNGKLNGKKEMVTPVIRASLEKQGYKIIGTHSGVKICRWTKSQLR 300
           IAGKAPSRKSTNVVETNGKLNGKKEMVTPVIRASLEKQGYKIIGTHSGVKICRWTKSQLR
Sbjct: 241 IAGKAPSRKSTNVVETNGKLNGKKEMVTPVIRASLEKQGYKIIGTHSGVKICRWTKSQLR 300

Query: 301 GRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVNSA 360
           GRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVNSA
Sbjct: 301 GRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVNSA 360

Query: 361 IDLHTKMIKQMKGVPGVTQEKLAEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRISTF 420
           IDLHTKMIKQMKGVPGVTQEKLAEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRISTF
Sbjct: 361 IDLHTKMIKQMKGVPGVTQEKLAEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRISTF 420

Query: 421 LVTNAQFPDKIKSLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNDKQQR 480
           LVTNAQFPDKIKSLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNDKQQR
Sbjct: 421 LVTNAQFPDKIKSLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNDKQQR 480

Query: 481 TVYRLTLVKGWNIEDIDAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENVPWHSDVKS 540
           TVYRLTLVKGWNIEDIDAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENVPWHSDVKS
Sbjct: 481 TVYRLTLVKGWNIEDIDAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENVPWHSDVKS 540

Query: 541 FSEALAAKSQGQYEVACEHVHSCCVLLAKTDKFRVNGQWFTWIDYEKFHDLVASGKPFDS 600
           FSEALAAKSQGQYEVACEHVHSCCVLLAKTDKFRVNGQWFTWIDYEKFHDLVASGKPFDS
Sbjct: 541 FSEALAAKSQGQYEVACEHVHSCCVLLAKTDKFRVNGQWFTWIDYEKFHDLVASGKPFDS 600

Query: 601 KDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHHRPKPMS 640
           KDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHHRPKPMS
Sbjct: 601 KDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHHRPKPMS 639

BLAST of MS016821 vs. NCBI nr
Match: XP_038884961.1 (S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase [Benincasa hispida] >XP_038884963.1 S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase [Benincasa hispida])

HSP 1 Score: 1225.7 bits (3170), Expect = 0.0e+00
Identity = 600/640 (93.75%), Postives = 618/640 (96.56%), Query Frame = 0

Query: 1   MSMSSLSARLTLLALLSASTFYFFYKSRRLRKLKLSLNPTVCSRRAKLFFISETGTSKAL 60
           MS SS SA+LTLLALLSAST Y FYKSRRLRKLKLSLNPT+ SRR KLFFIS+TGTSKAL
Sbjct: 1   MSFSSRSAKLTLLALLSASTLYCFYKSRRLRKLKLSLNPTLSSRRPKLFFISQTGTSKAL 60

Query: 61  AHRLLNVLNSNELAFDLVDPEDYEPEDLFKESLVLFVASTWEDGGPPPHAKFFANWLSES 120
           AHRLLNVLNSNEL FDLVDP+DYEPEDLFKE+LVLFVASTWEDGGPPPHAKF ANWLSES
Sbjct: 61  AHRLLNVLNSNELVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES 120

Query: 121 AEDFRVGSLLLSECKFGVFGVGSRAYGDTFNAVARGFSKQMRALGAKEILPVGEGDVDGG 180
           AEDFRVGSLLLSECKF VFGVGSRAYG+TFNAVAR FSK+M+ALGAKEIL VG+GDVDGG
Sbjct: 121 AEDFRVGSLLLSECKFAVFGVGSRAYGETFNAVARNFSKRMKALGAKEILAVGKGDVDGG 180

Query: 181 DIDKCFDDWSQQVLKVLKVDAVGSGTDLYSAIAGDSDAESV-EDDEEDADLGAEDIVDLE 240
           DID+CFDDWS+Q+LKVLKVDAVG+G DLYS I GDSDAESV ED+EEDADLG EDIVDLE
Sbjct: 181 DIDRCFDDWSRQLLKVLKVDAVGNGVDLYSGIGGDSDAESVEEDEEEDADLGEEDIVDLE 240

Query: 241 DIAGKAPSRKSTNVVETNGKLNGKKEMVTPVIRASLEKQGYKIIGTHSGVKICRWTKSQL 300
           DIAGK PSRKSTNVVETNGKLNGKK MVTPVIRASLEKQGYKIIG+HSGVKICRWTKSQL
Sbjct: 241 DIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQL 300

Query: 301 RGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVNS 360
           RGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVNS
Sbjct: 301 RGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVNS 360

Query: 361 AIDLHTKMIKQMKGVPGVTQEKLAEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRIST 420
           AIDLHTKMIKQMKGVPGVTQEKL EGLSPRHCALSLVGEPIMYPEINTLVDELHRRRIST
Sbjct: 361 AIDLHTKMIKQMKGVPGVTQEKLDEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRIST 420

Query: 421 FLVTNAQFPDKIKSLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNDKQQ 480
           FLVTNAQFPDKIK LKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNDKQQ
Sbjct: 421 FLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNDKQQ 480

Query: 481 RTVYRLTLVKGWNIEDIDAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENVPWHSDVK 540
           RTVYRLTLVKGWN EDIDAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENVPWHSDVK
Sbjct: 481 RTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENVPWHSDVK 540

Query: 541 SFSEALAAKSQGQYEVACEHVHSCCVLLAKTDKFRVNGQWFTWIDYEKFHDLVASGKPFD 600
           SFSEALAAKSQG+YEVACEHVHSCCVLLAKTDKFRV+GQWFTWIDYEKFHDLVASGKPFD
Sbjct: 541 SFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHDLVASGKPFD 600

Query: 601 SKDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHHRPKPMS 640
           SKDYMAPTPSWAVYGSDEGGFDP+QSRYRKERHHRPKP S
Sbjct: 601 SKDYMAPTPSWAVYGSDEGGFDPNQSRYRKERHHRPKPTS 640

BLAST of MS016821 vs. NCBI nr
Match: XP_022997485.1 (S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase [Cucurbita maxima])

HSP 1 Score: 1219.1 bits (3153), Expect = 0.0e+00
Identity = 594/639 (92.96%), Postives = 615/639 (96.24%), Query Frame = 0

Query: 1   MSMSSLSARLTLLALLSASTFYFFYKSRRLRKLKLSLNPTVCSRRAKLFFISETGTSKAL 60
           MS SS SA+L LLALLSAST Y FYKSRRLRKLKLSLNPT CSRR KLFFIS+TGTSKAL
Sbjct: 1   MSFSSRSAKLALLALLSASTLYCFYKSRRLRKLKLSLNPTGCSRRPKLFFISQTGTSKAL 60

Query: 61  AHRLLNVLNSNELAFDLVDPEDYEPEDLFKESLVLFVASTWEDGGPPPHAKFFANWLSES 120
           AHRLLNVLNSNELAFD+VDP+D+EPEDLFKE+LVLFVASTWEDGGPPPHAKF ANWLSES
Sbjct: 61  AHRLLNVLNSNELAFDIVDPKDFEPEDLFKEALVLFVASTWEDGGPPPHAKFLANWLSES 120

Query: 121 AEDFRVGSLLLSECKFGVFGVGSRAYGDTFNAVARGFSKQMRALGAKEILPVGEGDVDGG 180
           AEDFRVGSLLLS+CKF VFGVGSRAYG+TFNAVAR FSK+M+ALGAKEIL VGEGDVDGG
Sbjct: 121 AEDFRVGSLLLSQCKFAVFGVGSRAYGETFNAVARDFSKRMKALGAKEILAVGEGDVDGG 180

Query: 181 DIDKCFDDWSQQVLKVLKVDAVGSGTDLYSAIAGDSDAESVEDDEEDADLGAEDIVDLED 240
           DIDKCFDDWS+Q+LKVLKVDAVG+G DLYS I  D DAESVE+D+EDAD G EDIVDLED
Sbjct: 181 DIDKCFDDWSRQLLKVLKVDAVGTGADLYSGIDEDFDAESVEEDDEDADPGEEDIVDLED 240

Query: 241 IAGKAPSRKSTNVVETNGKLNGKKEMVTPVIRASLEKQGYKIIGTHSGVKICRWTKSQLR 300
           IAGK PSRKSTNVVETNGKLNGKK MVTPVIRASLEKQGYKIIG+HSGVKICRWTKSQLR
Sbjct: 241 IAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQLR 300

Query: 301 GRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVNSA 360
           GRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVNSA
Sbjct: 301 GRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVNSA 360

Query: 361 IDLHTKMIKQMKGVPGVTQEKLAEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRISTF 420
           IDLHTKMIKQMKGVPGVTQEKL EGLSPRHCALSLVGEPIMYPEINTLVDELHRRRISTF
Sbjct: 361 IDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRISTF 420

Query: 421 LVTNAQFPDKIKSLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNDKQQR 480
           LVTNAQFPDKIKSLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNDKQQR
Sbjct: 421 LVTNAQFPDKIKSLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNDKQQR 480

Query: 481 TVYRLTLVKGWNIEDIDAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENVPWHSDVKS 540
           TVYRLTLVKGWN EDIDAYSNLFS+G+PDFVEIKGVTYCGSSNTSKLTMENVPWHSDVKS
Sbjct: 481 TVYRLTLVKGWNTEDIDAYSNLFSLGKPDFVEIKGVTYCGSSNTSKLTMENVPWHSDVKS 540

Query: 541 FSEALAAKSQGQYEVACEHVHSCCVLLAKTDKFRVNGQWFTWIDYEKFHDLVASGKPFDS 600
           FSEALAAKSQG+YEVACEHVHSCCVLLAKTDKFRV+GQWFTWIDYEKFHDLVASGKPFDS
Sbjct: 541 FSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHDLVASGKPFDS 600

Query: 601 KDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHHRPKPMS 640
           KDYMAPTPSWAVYG+DEGGFDP QSRYRKERHHRPKP S
Sbjct: 601 KDYMAPTPSWAVYGADEGGFDPVQSRYRKERHHRPKPTS 639

BLAST of MS016821 vs. NCBI nr
Match: XP_023546392.1 (S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1213.7 bits (3139), Expect = 0.0e+00
Identity = 591/639 (92.49%), Postives = 614/639 (96.09%), Query Frame = 0

Query: 1   MSMSSLSARLTLLALLSASTFYFFYKSRRLRKLKLSLNPTVCSRRAKLFFISETGTSKAL 60
           MS SS SA+L LLALLSAST Y FYKSRRLRKLKLSLNPT CSRR KLFFIS+TGTSKAL
Sbjct: 1   MSFSSRSAKLALLALLSASTLYCFYKSRRLRKLKLSLNPTGCSRRPKLFFISQTGTSKAL 60

Query: 61  AHRLLNVLNSNELAFDLVDPEDYEPEDLFKESLVLFVASTWEDGGPPPHAKFFANWLSES 120
           AHRLLNVLNSNELAFD+VDP+D+EPEDLFKE+LVLFVASTWEDGGPPPHAKF A+WLSES
Sbjct: 61  AHRLLNVLNSNELAFDIVDPKDFEPEDLFKETLVLFVASTWEDGGPPPHAKFLASWLSES 120

Query: 121 AEDFRVGSLLLSECKFGVFGVGSRAYGDTFNAVARGFSKQMRALGAKEILPVGEGDVDGG 180
           AEDFRVGSLLLS+CKF VFGVGSRAYG+TFNAVAR FSK+M+ALGAKEIL VGEGDVDGG
Sbjct: 121 AEDFRVGSLLLSQCKFAVFGVGSRAYGETFNAVARDFSKRMKALGAKEILAVGEGDVDGG 180

Query: 181 DIDKCFDDWSQQVLKVLKVDAVGSGTDLYSAIAGDSDAESVEDDEEDADLGAEDIVDLED 240
           DIDKCFDDWS+Q+LKVLKVDAVG+G DLYS I  D DAESVE+D+EDAD G EDIVDLED
Sbjct: 181 DIDKCFDDWSRQLLKVLKVDAVGTGADLYSGIDEDFDAESVEEDDEDADSGEEDIVDLED 240

Query: 241 IAGKAPSRKSTNVVETNGKLNGKKEMVTPVIRASLEKQGYKIIGTHSGVKICRWTKSQLR 300
           IAGK PSRKSTNVVETNGKLNGKK MVTPVIRASLEKQGYKIIG+HSGVKICRWTKSQLR
Sbjct: 241 IAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQLR 300

Query: 301 GRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVNSA 360
           GRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVNSA
Sbjct: 301 GRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVNSA 360

Query: 361 IDLHTKMIKQMKGVPGVTQEKLAEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRISTF 420
           IDLH KMIKQMKGVPGVTQEKL EGLSPRHCALSLVGEPIMYPEINTLVDELH+RRISTF
Sbjct: 361 IDLHKKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHQRRISTF 420

Query: 421 LVTNAQFPDKIKSLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNDKQQR 480
           LVTNAQFPDKIKSLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNDKQQR
Sbjct: 421 LVTNAQFPDKIKSLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNDKQQR 480

Query: 481 TVYRLTLVKGWNIEDIDAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENVPWHSDVKS 540
           TVYRLTLVKGWN EDIDAYSNLFS+G+PDFVEIKGVTYCGSSNTSKLTMENVPWHSDVKS
Sbjct: 481 TVYRLTLVKGWNTEDIDAYSNLFSLGKPDFVEIKGVTYCGSSNTSKLTMENVPWHSDVKS 540

Query: 541 FSEALAAKSQGQYEVACEHVHSCCVLLAKTDKFRVNGQWFTWIDYEKFHDLVASGKPFDS 600
           FSEALAAKSQG+YEVACEHVHSCCVLLAKTDKFRV+GQWFTWIDYEKFHDLVASGKPFDS
Sbjct: 541 FSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHDLVASGKPFDS 600

Query: 601 KDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHHRPKPMS 640
           KDYMAPTPSWAVYG+DEGGFDP QSRYRKERHHRPKP S
Sbjct: 601 KDYMAPTPSWAVYGADEGGFDPVQSRYRKERHHRPKPTS 639

BLAST of MS016821 vs. NCBI nr
Match: XP_022962153.1 (S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase [Cucurbita moschata])

HSP 1 Score: 1211.1 bits (3132), Expect = 0.0e+00
Identity = 589/639 (92.18%), Postives = 614/639 (96.09%), Query Frame = 0

Query: 1   MSMSSLSARLTLLALLSASTFYFFYKSRRLRKLKLSLNPTVCSRRAKLFFISETGTSKAL 60
           MS+SS SA+L LLALLSAST Y FYKSRRLRKLKLSLNPT CSRR KLFFIS+TGTSKAL
Sbjct: 1   MSLSSRSAKLALLALLSASTLYCFYKSRRLRKLKLSLNPTGCSRRPKLFFISQTGTSKAL 60

Query: 61  AHRLLNVLNSNELAFDLVDPEDYEPEDLFKESLVLFVASTWEDGGPPPHAKFFANWLSES 120
           AHRLLNVLNSNELAFD+VDP+D+EPEDLFKE+LVLFVASTWEDGGPPPHAKF A+WLSES
Sbjct: 61  AHRLLNVLNSNELAFDIVDPKDFEPEDLFKETLVLFVASTWEDGGPPPHAKFLASWLSES 120

Query: 121 AEDFRVGSLLLSECKFGVFGVGSRAYGDTFNAVARGFSKQMRALGAKEILPVGEGDVDGG 180
           AEDFRVGSLLLS+CKF VFGVGSRAYG+TFNAVAR FSK+M+ALGAKEIL VGEGDVDGG
Sbjct: 121 AEDFRVGSLLLSQCKFAVFGVGSRAYGETFNAVARDFSKRMKALGAKEILAVGEGDVDGG 180

Query: 181 DIDKCFDDWSQQVLKVLKVDAVGSGTDLYSAIAGDSDAESVEDDEEDADLGAEDIVDLED 240
           DIDKCFDDWS+Q+LKVLKVDAVG+G DLYS I  D DAESVE+D+EDAD G EDIVDLED
Sbjct: 181 DIDKCFDDWSRQLLKVLKVDAVGTGADLYSGIDEDFDAESVEEDDEDADPGEEDIVDLED 240

Query: 241 IAGKAPSRKSTNVVETNGKLNGKKEMVTPVIRASLEKQGYKIIGTHSGVKICRWTKSQLR 300
           IAGK PSRKSTNVVETNGKLNGKK MVTPVIRASLEKQGYKIIG+HSGVKICRWTKSQLR
Sbjct: 241 IAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQLR 300

Query: 301 GRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVNSA 360
           GRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVNSA
Sbjct: 301 GRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVNSA 360

Query: 361 IDLHTKMIKQMKGVPGVTQEKLAEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRISTF 420
           IDLH KMIKQMKGVPGVTQEKL EGLSPRHCALSLVGEPIMYPEINTLVDELHRRRISTF
Sbjct: 361 IDLHKKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRISTF 420

Query: 421 LVTNAQFPDKIKSLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNDKQQR 480
           LVTNAQFPDKIKSLKPVTQLYVSVDAATK+SLKAIDRPLFGDFWERFIDSLKALNDKQQR
Sbjct: 421 LVTNAQFPDKIKSLKPVTQLYVSVDAATKDSLKAIDRPLFGDFWERFIDSLKALNDKQQR 480

Query: 481 TVYRLTLVKGWNIEDIDAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENVPWHSDVKS 540
           TVYRLTLVKGWN EDIDAYSNLFS+G+PDFVEIKGVTYCGSSNTSKLTMENVPWHSDVKS
Sbjct: 481 TVYRLTLVKGWNTEDIDAYSNLFSLGKPDFVEIKGVTYCGSSNTSKLTMENVPWHSDVKS 540

Query: 541 FSEALAAKSQGQYEVACEHVHSCCVLLAKTDKFRVNGQWFTWIDYEKFHDLVASGKPFDS 600
           FSEALAAKSQG+YEVACEHVHSCCVLLAKTDKFR++GQWFTWIDYEKFH LVASGKPFDS
Sbjct: 541 FSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRIDGQWFTWIDYEKFHALVASGKPFDS 600

Query: 601 KDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHHRPKPMS 640
           KDYMAPTPSWAVYG+DEGGFDP QSRYRKERHHRPKP S
Sbjct: 601 KDYMAPTPSWAVYGADEGGFDPVQSRYRKERHHRPKPTS 639

BLAST of MS016821 vs. ExPASy Swiss-Prot
Match: Q8RXN5 (S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase OS=Arabidopsis thaliana OX=3702 GN=TYW1 PE=2 SV=1)

HSP 1 Score: 953.0 bits (2462), Expect = 1.7e-276
Identity = 469/642 (73.05%), Postives = 538/642 (83.80%), Query Frame = 0

Query: 4   SSLSARLTLLALLSASTFYFFYKSRRLRKLK-LSLNP--TVCSRRAKLFFISETGTSKAL 63
           SS+  RL  +ALLSA+TFY  +K RRL+ LK LSLNP  T+ + R K+FFIS+TGT+KAL
Sbjct: 5   SSVRVRLAFVALLSATTFYCIHKYRRLKHLKNLSLNPSSTLKASRGKIFFISQTGTAKAL 64

Query: 64  AHRLLNVLNSNELAFDLVDPEDYEPEDLFKESLVLFVASTWEDGGPPPHAKFFANWLSES 123
           A RL  +  SN++AFD+VDP  YEPEDL KE+LVLF+ASTW+ G PP + +F  NWL ES
Sbjct: 65  AQRLHELCASNDIAFDIVDPHSYEPEDLPKETLVLFIASTWDGGKPPKNGEFLVNWLGES 124

Query: 124 AEDFRVGSLLLSECKFGVFGVGSRAYGDTFNAVARGFSKQMRALGAKEILPVGEGDVDGG 183
           AEDFRVGSLLLS+CKF VFGVGSRAYG+++NAVA+  S +M  LG  E++PVGEGDVD G
Sbjct: 125 AEDFRVGSLLLSDCKFAVFGVGSRAYGESYNAVAKELSSRMIGLGGLEMIPVGEGDVDDG 184

Query: 184 DIDKCFDDWSQQVLKVLKVDAVGSGTDLYSAI-AGDSDAESVED-DEEDADLGAE-DIVD 243
           ++D+ F DW   V++VLK  +      +   I A + D E  +  DEED D  A+  IVD
Sbjct: 185 ELDRAFQDWCDGVIRVLKGGSAQETNGVSQQIGAVEDDLEYYDSTDEEDEDNDADGGIVD 244

Query: 244 LEDIAGKAPSRKSTNVVETNGKLNGKKEMVTPVIRASLEKQGYKIIGTHSGVKICRWTKS 303
           LEDIAGKAPS+++  V  T  K++GKKEMVTPVIRASL KQGYKIIG+HSGVKICRWTKS
Sbjct: 245 LEDIAGKAPSKRNGVVKVT--KVDGKKEMVTPVIRASLTKQGYKIIGSHSGVKICRWTKS 304

Query: 304 QLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIV 363
           QLRGRGGCYKHSFYGIESHRCME TPSLACANKCVFCWRHHTNPVGKSWQW+MD+P  IV
Sbjct: 305 QLRGRGGCYKHSFYGIESHRCMETTPSLACANKCVFCWRHHTNPVGKSWQWKMDEPSVIV 364

Query: 364 NSAIDLHTKMIKQMKGVPGVTQEKLAEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRI 423
             A+DLH  MIKQMKGVPGVT EKL EGL+PRHCALSLVGEPIMYPEIN LVDELH RRI
Sbjct: 365 KGALDLHKNMIKQMKGVPGVTPEKLQEGLNPRHCALSLVGEPIMYPEINALVDELHGRRI 424

Query: 424 STFLVTNAQFPDKIKSLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNDK 483
           STFLVTNAQFP+KI  +KP+TQLYVSVDAATKESLKAIDRPLF DFWERFIDSLKAL +K
Sbjct: 425 STFLVTNAQFPEKILMMKPITQLYVSVDAATKESLKAIDRPLFADFWERFIDSLKALQEK 484

Query: 484 QQRTVYRLTLVKGWNIEDIDAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENVPWHSD 543
           QQRTVYRLTLVKGWN E++DAY NLFSIG+PDF+EIKGVTYCGSS TSKLTMENVPWH+D
Sbjct: 485 QQRTVYRLTLVKGWNTEELDAYFNLFSIGKPDFIEIKGVTYCGSSATSKLTMENVPWHTD 544

Query: 544 VKSFSEALAAKSQGQYEVACEHVHSCCVLLAKTDKFRVNGQWFTWIDYEKFHDLVASGKP 603
           VK+FSEAL+ KS G+YEVACEH HSCCVLL +T+KF+V+G+WFTWIDYEKFHDLVASG+P
Sbjct: 545 VKAFSEALSLKSNGEYEVACEHAHSCCVLLGRTEKFKVDGKWFTWIDYEKFHDLVASGEP 604

Query: 604 FDSKDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHHRPKPMS 640
           F S DYMA TPSWAVYG+ EGGFDP Q RY+KER+H PKP +
Sbjct: 605 FTSTDYMAQTPSWAVYGAQEGGFDPGQLRYKKERNHPPKPQA 644

BLAST of MS016821 vs. ExPASy Swiss-Prot
Match: Q8H8N3 (S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase OS=Oryza sativa subsp. japonica OX=39947 GN=TYW1 PE=2 SV=1)

HSP 1 Score: 883.6 bits (2282), Expect = 1.3e-255
Identity = 437/641 (68.17%), Postives = 512/641 (79.88%), Query Frame = 0

Query: 4   SSLSARLTLLALLSASTFYFFYKSRRLRKLKLSLNP--TVCSRRAKLFFISETGTSKALA 63
           +S ++ L LL LLS+S+ +F YKS RLR+      P     +    L + S TGTSKALA
Sbjct: 12  ASTTSHLALLLLLSSSSVFFLYKSLRLRRNNPPSPPPGQGPAPTPTLLYASATGTSKALA 71

Query: 64  HRLLNVLNSNELAFDLVDPEDYEPEDLFKESLVLFVASTWEDGGPPPHAKFFANWLSESA 123
             L   L    +     D   ++P+DL    L+L V  T + G PPP A F A WL ESA
Sbjct: 72  AGLSRRLAEAGVTAHPADAAAFDPDDLPSLPLLLLVLPTHDGGAPPPAAAFLARWLEESA 131

Query: 124 EDFRVGSLLLSECKFGVFGVGSRAYGDTFNAVARGFSKQMRALGAKEILPVGEGDVDGGD 183
            DFR G+ LLS  +F VFGVGSRAYG+TFNA AR FS+ +RALGA E++ VGEGDVDGGD
Sbjct: 132 ADFRAGAALLSGLRFAVFGVGSRAYGETFNAAARSFSRWLRALGAAEVVAVGEGDVDGGD 191

Query: 184 IDKCFDDWSQQVLKVLKVDAVGSGTDLYSAIAGDSDA-ESVEDDEEDADLGAEDI----V 243
           ++  F++W  +V++V+K + +G G +      G+SD  + +E++E D D   E++    V
Sbjct: 192 LEVVFEEWCGRVVRVVKGEEIGEGHN------GESDGFDELEEEESDDDDDEEEVDGGEV 251

Query: 244 DLEDIAGKAP-SRKSTNVVE---TNGKLNGKKEMVTPVIRASLEKQGYKIIGTHSGVKIC 303
           D+EDIAGKAP +R+    VE   +NG  NG ++MVTP+IR SLEKQGYKIIG+HSGVKIC
Sbjct: 252 DMEDIAGKAPAARRRNGKVEGALSNGGENGVRDMVTPIIRTSLEKQGYKIIGSHSGVKIC 311

Query: 304 RWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDD 363
           RWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSW+W+MDD
Sbjct: 312 RWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWKWKMDD 371

Query: 364 PLDIVNSAIDLHTKMIKQMKGVPGVTQEKLAEGLSPRHCALSLVGEPIMYPEINTLVDEL 423
           PLDIVN+AID HTKM+KQMKGVPGV  E+LAEGLSPRHCALSLVGEPIMYPEIN L+DEL
Sbjct: 372 PLDIVNAAIDQHTKMVKQMKGVPGVKPERLAEGLSPRHCALSLVGEPIMYPEINVLIDEL 431

Query: 424 HRRRISTFLVTNAQFPDKIKSLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLK 483
           HRR ISTFLVTNAQFPDKIK+LKP+TQLYVSVDAATKESLKA+DRPLF DFWERF+DSLK
Sbjct: 432 HRRHISTFLVTNAQFPDKIKTLKPITQLYVSVDAATKESLKAVDRPLFSDFWERFLDSLK 491

Query: 484 ALNDKQQRTVYRLTLVKGWNIEDIDAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENV 543
           +L+DK QRTVYRLTLVKGWN E+ID Y+ L S+G+PDF+EIKGVTYCGSS TSKLTMENV
Sbjct: 492 SLHDKDQRTVYRLTLVKGWNAEEIDGYAKLLSLGQPDFIEIKGVTYCGSSATSKLTMENV 551

Query: 544 PWHSDVKSFSEALAAKSQGQYEVACEHVHSCCVLLAKTDKFRVNGQWFTWIDYEKFHDLV 603
           PWHSDVK FSEALA KS G YEVACEH HSCCVLLAK DKF++NG+W TWIDY++FH+LV
Sbjct: 552 PWHSDVKDFSEALALKSGGVYEVACEHAHSCCVLLAKVDKFKINGKWHTWIDYDRFHELV 611

Query: 604 ASGKPFDSKDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHH 634
            SGKPF S+DYMA TPSWAVYG++EGGFDPDQSRY+KER H
Sbjct: 612 TSGKPFRSQDYMALTPSWAVYGAEEGGFDPDQSRYKKERRH 646

BLAST of MS016821 vs. ExPASy Swiss-Prot
Match: Q2KHP8 (S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase TYW1 OS=Xenopus laevis OX=8355 GN=tyw1 PE=2 SV=1)

HSP 1 Score: 615.9 bits (1587), Expect = 5.1e-175
Identity = 314/646 (48.61%), Postives = 419/646 (64.86%), Query Frame = 0

Query: 47  KLFFISETGTSKALAHRLLNVLNSNELAFDLVDPEDYEPEDLFKE-----SLVLFVASTW 106
           K+F+ S+TGTSK  AH L   +    L  +L++ ++Y+P+D   E     ++ +F+ +T+
Sbjct: 84  KIFYGSQTGTSKGFAHLLAEEVTLLGLPVELINMKEYDPDDNLVEETTSKNICVFLVATY 143

Query: 107 EDGGPPPHAKFFANWLSESAEDFRVGSLLLSECKFGVFGVGSRAYGDTFNAVARGFSKQM 166
            DG PP  A++F  WL E++ DFR G   L   ++ VFG+G+  Y   +N V +   K +
Sbjct: 144 TDGKPPESAEWFCKWLEEASNDFRFGKTYLKGMRYAVFGLGNSVYSTHYNTVGKNIDKWL 203

Query: 167 RALGAKEILPVGEGDVD-----GGDIDKCFDDWSQQVLKVLKVDAVGSGTDLYSAI---- 226
             L A  ++   EGD +      G I+  F+ W ++ L  LK    G             
Sbjct: 204 WMLSANRVMTRAEGDCNVVKSKHGSIESDFEAWKRKFLNRLKALLSGEKKPCSGKCKKGK 263

Query: 227 ---AGDSDAESVEDDEEDADLGAE-------------------------DIVDLEDIAGK 286
                 S  ESVE++EE+ +  +E                          ++D+ED+ GK
Sbjct: 264 CKSKKKSSIESVEEEEEEEEKHSEHEDTEDDTFETSSDSEPEEHGEPGSGLIDVEDL-GK 323

Query: 287 APS--RKSTNVVETNGKLNGK------------KEMVTPVIRASLEKQGYKIIGTHSGVK 346
           A S  +KS    ++N +L               +EM+TP ++ +L KQGY++IG+HSGVK
Sbjct: 324 AMSNMKKSKREHDSNTELGKSLQGDEAKEKEEPREMITPALQEALTKQGYRLIGSHSGVK 383

Query: 347 ICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEM 406
           +CRWTKS LRGRGGCYKH+FYGIESHRCME TPSLACANKCVFCWRHHTNPVG  W+W+M
Sbjct: 384 LCRWTKSMLRGRGGCYKHTFYGIESHRCMETTPSLACANKCVFCWRHHTNPVGTEWRWKM 443

Query: 407 DDPLDIVNSAIDLHTKMIKQMKGVPGVTQEKLAEGLSPRHCALSLVGEPIMYPEINTLVD 466
           D P  I+  AI  H  MIKQ KGVPGV  E+  EGL+ +HCALSLVGEPIMYPEINT + 
Sbjct: 444 DQPEMILEEAILNHQNMIKQFKGVPGVKPERFEEGLAVKHCALSLVGEPIMYPEINTFLR 503

Query: 467 ELHRRRISTFLVTNAQFPDKIKSLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDS 526
            LH + IS+FLVTNAQFP++I+SL+PVTQLYVSVDA+TK+SLK IDRPLF DFW+RFIDS
Sbjct: 504 LLHNQHISSFLVTNAQFPEEIRSLEPVTQLYVSVDASTKDSLKKIDRPLFKDFWQRFIDS 563

Query: 527 LKALNDKQQRTVYRLTLVKGWNIEDIDAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTME 586
           LKAL +KQQRTVYRLTLVK WN++++ AY++L ++G PDF+E+KGVTYCG S+ S LTM 
Sbjct: 564 LKALAEKQQRTVYRLTLVKAWNVDELKAYADLVALGLPDFIEVKGVTYCGESSASNLTMA 623

Query: 587 NVPWHSDVKSFSEALAAKSQGQYEVACEHVHSCCVLLAKTDKFRVNGQWFTWIDYEKFHD 631
           NVPWH +V  F E L       YEVACEH HS C+L+A   KF++NG+W TWIDYE+F +
Sbjct: 624 NVPWHEEVIRFVEEL-VDLLPDYEVACEHEHSNCMLIAH-KKFKINGEWCTWIDYERFQE 683

BLAST of MS016821 vs. ExPASy Swiss-Prot
Match: Q08C92 (S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase TYW1 OS=Danio rerio OX=7955 GN=tyw1 PE=2 SV=1)

HSP 1 Score: 611.7 bits (1576), Expect = 9.5e-174
Identity = 307/644 (47.67%), Postives = 419/644 (65.06%), Query Frame = 0

Query: 47  KLFFISETGTSKALAHRLLNVLNSNELAFDLVDPEDYEPEDLFKES-----LVLFVASTW 106
           K+F+ S+TGT+K  A  L   + +  +  +++D +D++PED   E      + +F+ +T+
Sbjct: 80  KVFYGSQTGTAKGFAKELAEDVIAQGIQCEVIDMKDFDPEDRLAEECTSKIICVFLVATY 139

Query: 107 EDGGPPPHAKFFANWLSESAEDFRVGSLLLSECKFGVFGVGSRAYGDTFNAVARGFSKQM 166
            DG P   A++F  WL E++ DFR G   L   ++ VFG+G+  Y   FN V++   K +
Sbjct: 140 TDGQPTESAEWFCKWLEEASTDFRYGKTYLKGMRYAVFGLGNSVYVGHFNTVSKSIDKWL 199

Query: 167 RALGAKEILPVGEGDVD-----GGDIDKCFDDWSQQVLKVLKVDAVG------------- 226
             L A  I+  GEGD +      G +   F  W  + L  L+  A G             
Sbjct: 200 WMLSAARIMTRGEGDCNVVKSRHGSVQADFQVWKGKFLNRLQALAKGEKKACSGNCKKAS 259

Query: 227 ---------SGTDLYSAIAGDSDAESVEDDEEDADLGAED------IVDLEDIAG----- 286
                       D +S    ++  E + +   D +  +ED      ++D+ED+       
Sbjct: 260 CKNKKKHKEEAEDNHSLAEKNNSEEELMESSSDEESSSEDEKSHGSVIDMEDLGNVMNHM 319

Query: 287 -KAPSR-----KSTNVVETNGKLNG-----KKEMVTPVIRASLEKQGYKIIGTHSGVKIC 346
            KA  R     + +  V+ NG+        ++EM+TP +R SL KQGYK+IG+HSGVK+ 
Sbjct: 320 KKAKQRMEEDEEDSQRVKQNGERKSECEEERREMITPALRDSLTKQGYKLIGSHSGVKLR 379

Query: 347 RWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDD 406
           RWTKS LRGRGGCYKH+FYGIESHRCME TPSLACANKCVFCWRHHTNPVG  W+W+MD 
Sbjct: 380 RWTKSMLRGRGGCYKHTFYGIESHRCMETTPSLACANKCVFCWRHHTNPVGTEWRWKMDP 439

Query: 407 PLDIVNSAIDLHTKMIKQMKGVPGVTQEKLAEGLSPRHCALSLVGEPIMYPEINTLVDEL 466
              I+  A++ H  MI+Q +GVPGV  E+  EGL+ +HCALSLVGEPIMYPEIN+ +  L
Sbjct: 440 AEKIIQEAMENHRNMIRQFRGVPGVRPERFEEGLTVKHCALSLVGEPIMYPEINSFLKLL 499

Query: 467 HRRRISTFLVTNAQFPDKIKSLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLK 526
           H++ IS+FLVTNAQFP++I+SL PVTQLYVSVDA+TK+SLK IDRPLF DFW+RF+DSL+
Sbjct: 500 HQQNISSFLVTNAQFPEEIRSLVPVTQLYVSVDASTKDSLKKIDRPLFKDFWQRFLDSLR 559

Query: 527 ALNDKQQRTVYRLTLVKGWNIEDIDAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENV 586
           AL +KQQRTVYRLTLVK WN++++ AY++L ++G+PDF+E+KGVTYCG S+ S LTM NV
Sbjct: 560 ALGEKQQRTVYRLTLVKAWNVDELKAYADLIALGQPDFIEVKGVTYCGESSASSLTMANV 619

Query: 587 PWHSDVKSFSEALAAKSQGQYEVACEHVHSCCVLLAKTDKFRVNGQWFTWIDYEKFHDLV 631
           PWH +V  F + L A     YE+ACEH HS C+LLA   KF+V+G+W+TWIDYE+F +L+
Sbjct: 620 PWHEEVIYFVQQL-ANLLPDYEIACEHEHSNCLLLA-NHKFKVDGEWWTWIDYERFQELI 679

BLAST of MS016821 vs. ExPASy Swiss-Prot
Match: Q9NV66 (S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase TYW1 OS=Homo sapiens OX=9606 GN=TYW1 PE=2 SV=2)

HSP 1 Score: 602.8 bits (1553), Expect = 4.4e-171
Identity = 305/646 (47.21%), Postives = 414/646 (64.09%), Query Frame = 0

Query: 47  KLFFISETGTSKALAHRLLNVLNSNELAFDLVDPEDYEPED-----LFKESLVLFVASTW 106
           K+F+ S+TGT+K  A  L   + S +L   +++ ++Y+P+D     +  +++ +F+ +T+
Sbjct: 80  KIFYGSQTGTAKGFATVLAEAVTSLDLPVAIINLKEYDPDDHLIEEVTSKNVCVFLVATY 139

Query: 107 EDGGPPPHAKFFANWLSESAEDFRVGSLLLSECKFGVFGVGSRAYGDTFNAVARGFSKQM 166
            DG P   A++F  WL E++ DFR G   L   ++ VFG+G+ AY   FN V +   K +
Sbjct: 140 TDGLPTESAEWFCKWLEEASIDFRFGKTYLKGMRYAVFGLGNSAYASHFNKVGKNVDKWL 199

Query: 167 RALGAKEILPVGEGDVD-----GGDIDKCFDDWSQQVLKVL------------------- 226
             LGA  ++  GEGD D      G I+  F  W  + +  L                   
Sbjct: 200 WMLGAHRVMSRGEGDCDVVKSKHGSIEADFRAWKTKFISQLQALQKGERKKSCGGHCKKG 259

Query: 227 KVDAVGSGTDLYSAIAGDSDAESVEDDEE--------DADLGAED------IVDLEDIAG 286
           K ++   G++     + + D     D EE        + + G ED      IVD+ED+  
Sbjct: 260 KCESHQHGSEEREEGSHEQDELHHRDTEEEEPFESSSEEEFGGEDHQSLNSIVDVEDLGK 319

Query: 287 -----KAPSRKSTNVVETNGKLNG--------KKEMVTPVIRASLEKQGYKIIGTHSGVK 346
                K   R+     E +G            ++ M+TP +R +L KQGY++IG+HSGVK
Sbjct: 320 IMDHVKKEKREKEQQEEKSGLFRNMGRNEDGERRAMITPALREALTKQGYQLIGSHSGVK 379

Query: 347 ICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEM 406
           +CRWTKS LRGRGGCYKH+FYGIESHRCME TPSLACANKCVFCWRHHTNPVG  W+W+M
Sbjct: 380 LCRWTKSMLRGRGGCYKHTFYGIESHRCMETTPSLACANKCVFCWRHHTNPVGTEWRWKM 439

Query: 407 DDPLDIVNSAIDLHTKMIKQMKGVPGVTQEKLAEGLSPRHCALSLVGEPIMYPEINTLVD 466
           D P  I+  AI+ H  MIKQ KGVPGV  E+  EG++ +HCALSLVGEPIMYPEIN  + 
Sbjct: 440 DQPEMILKEAIENHQNMIKQFKGVPGVKAERFEEGMTVKHCALSLVGEPIMYPEINRFLK 499

Query: 467 ELHRRRISTFLVTNAQFPDKIKSLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDS 526
            LH+ +IS+FLVTNAQFP +I++L+PVTQLYVSVDA+TK+SLK IDRPLF DFW+RF+DS
Sbjct: 500 LLHQCKISSFLVTNAQFPAEIRNLEPVTQLYVSVDASTKDSLKKIDRPLFKDFWQRFLDS 559

Query: 527 LKALNDKQQRTVYRLTLVKGWNIEDIDAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTME 586
           LKAL  KQQRTVYRLTLVK WN++++ AY+ L S+G PDF+E+KGVTYCG S+ S LTM 
Sbjct: 560 LKALAVKQQRTVYRLTLVKAWNVDELQAYAQLVSLGNPDFIEVKGVTYCGESSASSLTMA 619

Query: 587 NVPWHSDVKSFSEALAAKSQGQYEVACEHVHSCCVLLAKTDKFRVNGQWFTWIDYEKFHD 631
           +VPWH +V  F   L      +YE+ACEH HS C+L+A   KF++ G+W+TWIDY +F +
Sbjct: 620 HVPWHEEVVQFVHELVDLIP-EYEIACEHEHSNCLLIAHR-KFKIGGEWWTWIDYNRFQE 679

BLAST of MS016821 vs. ExPASy TrEMBL
Match: A0A6J1CT11 (tRNA 4-demethylwyosine synthase (AdoMet-dependent) OS=Momordica charantia OX=3673 GN=LOC111014045 PE=3 SV=1)

HSP 1 Score: 1300.0 bits (3363), Expect = 0.0e+00
Identity = 638/639 (99.84%), Postives = 638/639 (99.84%), Query Frame = 0

Query: 1   MSMSSLSARLTLLALLSASTFYFFYKSRRLRKLKLSLNPTVCSRRAKLFFISETGTSKAL 60
           MSMSSLSARLTLLALLSASTFYFFYKSRRLRKLKLSLNPTVCSRRAKLFFISETGTSKAL
Sbjct: 1   MSMSSLSARLTLLALLSASTFYFFYKSRRLRKLKLSLNPTVCSRRAKLFFISETGTSKAL 60

Query: 61  AHRLLNVLNSNELAFDLVDPEDYEPEDLFKESLVLFVASTWEDGGPPPHAKFFANWLSES 120
           AHRLLNVLNSNELAFDLVDPEDYEPEDLFKESLVL VASTWEDGGPPPHAKFFANWLSES
Sbjct: 61  AHRLLNVLNSNELAFDLVDPEDYEPEDLFKESLVLLVASTWEDGGPPPHAKFFANWLSES 120

Query: 121 AEDFRVGSLLLSECKFGVFGVGSRAYGDTFNAVARGFSKQMRALGAKEILPVGEGDVDGG 180
           AEDFRVGSLLLSECKFGVFGVGSRAYGDTFNAVARGFSKQMRALGAKEILPVGEGDVDGG
Sbjct: 121 AEDFRVGSLLLSECKFGVFGVGSRAYGDTFNAVARGFSKQMRALGAKEILPVGEGDVDGG 180

Query: 181 DIDKCFDDWSQQVLKVLKVDAVGSGTDLYSAIAGDSDAESVEDDEEDADLGAEDIVDLED 240
           DIDKCFDDWSQQVLKVLKVDAVGSGTDLYSAIAGDSDAESVEDDEEDADLGAEDIVDLED
Sbjct: 181 DIDKCFDDWSQQVLKVLKVDAVGSGTDLYSAIAGDSDAESVEDDEEDADLGAEDIVDLED 240

Query: 241 IAGKAPSRKSTNVVETNGKLNGKKEMVTPVIRASLEKQGYKIIGTHSGVKICRWTKSQLR 300
           IAGKAPSRKSTNVVETNGKLNGKKEMVTPVIRASLEKQGYKIIGTHSGVKICRWTKSQLR
Sbjct: 241 IAGKAPSRKSTNVVETNGKLNGKKEMVTPVIRASLEKQGYKIIGTHSGVKICRWTKSQLR 300

Query: 301 GRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVNSA 360
           GRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVNSA
Sbjct: 301 GRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVNSA 360

Query: 361 IDLHTKMIKQMKGVPGVTQEKLAEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRISTF 420
           IDLHTKMIKQMKGVPGVTQEKLAEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRISTF
Sbjct: 361 IDLHTKMIKQMKGVPGVTQEKLAEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRISTF 420

Query: 421 LVTNAQFPDKIKSLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNDKQQR 480
           LVTNAQFPDKIKSLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNDKQQR
Sbjct: 421 LVTNAQFPDKIKSLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNDKQQR 480

Query: 481 TVYRLTLVKGWNIEDIDAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENVPWHSDVKS 540
           TVYRLTLVKGWNIEDIDAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENVPWHSDVKS
Sbjct: 481 TVYRLTLVKGWNIEDIDAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENVPWHSDVKS 540

Query: 541 FSEALAAKSQGQYEVACEHVHSCCVLLAKTDKFRVNGQWFTWIDYEKFHDLVASGKPFDS 600
           FSEALAAKSQGQYEVACEHVHSCCVLLAKTDKFRVNGQWFTWIDYEKFHDLVASGKPFDS
Sbjct: 541 FSEALAAKSQGQYEVACEHVHSCCVLLAKTDKFRVNGQWFTWIDYEKFHDLVASGKPFDS 600

Query: 601 KDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHHRPKPMS 640
           KDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHHRPKPMS
Sbjct: 601 KDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHHRPKPMS 639

BLAST of MS016821 vs. ExPASy TrEMBL
Match: A0A6J1K9S7 (tRNA 4-demethylwyosine synthase (AdoMet-dependent) OS=Cucurbita maxima OX=3661 GN=LOC111492394 PE=3 SV=1)

HSP 1 Score: 1219.1 bits (3153), Expect = 0.0e+00
Identity = 594/639 (92.96%), Postives = 615/639 (96.24%), Query Frame = 0

Query: 1   MSMSSLSARLTLLALLSASTFYFFYKSRRLRKLKLSLNPTVCSRRAKLFFISETGTSKAL 60
           MS SS SA+L LLALLSAST Y FYKSRRLRKLKLSLNPT CSRR KLFFIS+TGTSKAL
Sbjct: 1   MSFSSRSAKLALLALLSASTLYCFYKSRRLRKLKLSLNPTGCSRRPKLFFISQTGTSKAL 60

Query: 61  AHRLLNVLNSNELAFDLVDPEDYEPEDLFKESLVLFVASTWEDGGPPPHAKFFANWLSES 120
           AHRLLNVLNSNELAFD+VDP+D+EPEDLFKE+LVLFVASTWEDGGPPPHAKF ANWLSES
Sbjct: 61  AHRLLNVLNSNELAFDIVDPKDFEPEDLFKEALVLFVASTWEDGGPPPHAKFLANWLSES 120

Query: 121 AEDFRVGSLLLSECKFGVFGVGSRAYGDTFNAVARGFSKQMRALGAKEILPVGEGDVDGG 180
           AEDFRVGSLLLS+CKF VFGVGSRAYG+TFNAVAR FSK+M+ALGAKEIL VGEGDVDGG
Sbjct: 121 AEDFRVGSLLLSQCKFAVFGVGSRAYGETFNAVARDFSKRMKALGAKEILAVGEGDVDGG 180

Query: 181 DIDKCFDDWSQQVLKVLKVDAVGSGTDLYSAIAGDSDAESVEDDEEDADLGAEDIVDLED 240
           DIDKCFDDWS+Q+LKVLKVDAVG+G DLYS I  D DAESVE+D+EDAD G EDIVDLED
Sbjct: 181 DIDKCFDDWSRQLLKVLKVDAVGTGADLYSGIDEDFDAESVEEDDEDADPGEEDIVDLED 240

Query: 241 IAGKAPSRKSTNVVETNGKLNGKKEMVTPVIRASLEKQGYKIIGTHSGVKICRWTKSQLR 300
           IAGK PSRKSTNVVETNGKLNGKK MVTPVIRASLEKQGYKIIG+HSGVKICRWTKSQLR
Sbjct: 241 IAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQLR 300

Query: 301 GRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVNSA 360
           GRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVNSA
Sbjct: 301 GRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVNSA 360

Query: 361 IDLHTKMIKQMKGVPGVTQEKLAEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRISTF 420
           IDLHTKMIKQMKGVPGVTQEKL EGLSPRHCALSLVGEPIMYPEINTLVDELHRRRISTF
Sbjct: 361 IDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRISTF 420

Query: 421 LVTNAQFPDKIKSLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNDKQQR 480
           LVTNAQFPDKIKSLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNDKQQR
Sbjct: 421 LVTNAQFPDKIKSLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNDKQQR 480

Query: 481 TVYRLTLVKGWNIEDIDAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENVPWHSDVKS 540
           TVYRLTLVKGWN EDIDAYSNLFS+G+PDFVEIKGVTYCGSSNTSKLTMENVPWHSDVKS
Sbjct: 481 TVYRLTLVKGWNTEDIDAYSNLFSLGKPDFVEIKGVTYCGSSNTSKLTMENVPWHSDVKS 540

Query: 541 FSEALAAKSQGQYEVACEHVHSCCVLLAKTDKFRVNGQWFTWIDYEKFHDLVASGKPFDS 600
           FSEALAAKSQG+YEVACEHVHSCCVLLAKTDKFRV+GQWFTWIDYEKFHDLVASGKPFDS
Sbjct: 541 FSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHDLVASGKPFDS 600

Query: 601 KDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHHRPKPMS 640
           KDYMAPTPSWAVYG+DEGGFDP QSRYRKERHHRPKP S
Sbjct: 601 KDYMAPTPSWAVYGADEGGFDPVQSRYRKERHHRPKPTS 639

BLAST of MS016821 vs. ExPASy TrEMBL
Match: A0A6J1HDZ5 (tRNA 4-demethylwyosine synthase (AdoMet-dependent) OS=Cucurbita moschata OX=3662 GN=LOC111462691 PE=3 SV=1)

HSP 1 Score: 1211.1 bits (3132), Expect = 0.0e+00
Identity = 589/639 (92.18%), Postives = 614/639 (96.09%), Query Frame = 0

Query: 1   MSMSSLSARLTLLALLSASTFYFFYKSRRLRKLKLSLNPTVCSRRAKLFFISETGTSKAL 60
           MS+SS SA+L LLALLSAST Y FYKSRRLRKLKLSLNPT CSRR KLFFIS+TGTSKAL
Sbjct: 1   MSLSSRSAKLALLALLSASTLYCFYKSRRLRKLKLSLNPTGCSRRPKLFFISQTGTSKAL 60

Query: 61  AHRLLNVLNSNELAFDLVDPEDYEPEDLFKESLVLFVASTWEDGGPPPHAKFFANWLSES 120
           AHRLLNVLNSNELAFD+VDP+D+EPEDLFKE+LVLFVASTWEDGGPPPHAKF A+WLSES
Sbjct: 61  AHRLLNVLNSNELAFDIVDPKDFEPEDLFKETLVLFVASTWEDGGPPPHAKFLASWLSES 120

Query: 121 AEDFRVGSLLLSECKFGVFGVGSRAYGDTFNAVARGFSKQMRALGAKEILPVGEGDVDGG 180
           AEDFRVGSLLLS+CKF VFGVGSRAYG+TFNAVAR FSK+M+ALGAKEIL VGEGDVDGG
Sbjct: 121 AEDFRVGSLLLSQCKFAVFGVGSRAYGETFNAVARDFSKRMKALGAKEILAVGEGDVDGG 180

Query: 181 DIDKCFDDWSQQVLKVLKVDAVGSGTDLYSAIAGDSDAESVEDDEEDADLGAEDIVDLED 240
           DIDKCFDDWS+Q+LKVLKVDAVG+G DLYS I  D DAESVE+D+EDAD G EDIVDLED
Sbjct: 181 DIDKCFDDWSRQLLKVLKVDAVGTGADLYSGIDEDFDAESVEEDDEDADPGEEDIVDLED 240

Query: 241 IAGKAPSRKSTNVVETNGKLNGKKEMVTPVIRASLEKQGYKIIGTHSGVKICRWTKSQLR 300
           IAGK PSRKSTNVVETNGKLNGKK MVTPVIRASLEKQGYKIIG+HSGVKICRWTKSQLR
Sbjct: 241 IAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQLR 300

Query: 301 GRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVNSA 360
           GRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVNSA
Sbjct: 301 GRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVNSA 360

Query: 361 IDLHTKMIKQMKGVPGVTQEKLAEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRISTF 420
           IDLH KMIKQMKGVPGVTQEKL EGLSPRHCALSLVGEPIMYPEINTLVDELHRRRISTF
Sbjct: 361 IDLHKKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRISTF 420

Query: 421 LVTNAQFPDKIKSLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNDKQQR 480
           LVTNAQFPDKIKSLKPVTQLYVSVDAATK+SLKAIDRPLFGDFWERFIDSLKALNDKQQR
Sbjct: 421 LVTNAQFPDKIKSLKPVTQLYVSVDAATKDSLKAIDRPLFGDFWERFIDSLKALNDKQQR 480

Query: 481 TVYRLTLVKGWNIEDIDAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENVPWHSDVKS 540
           TVYRLTLVKGWN EDIDAYSNLFS+G+PDFVEIKGVTYCGSSNTSKLTMENVPWHSDVKS
Sbjct: 481 TVYRLTLVKGWNTEDIDAYSNLFSLGKPDFVEIKGVTYCGSSNTSKLTMENVPWHSDVKS 540

Query: 541 FSEALAAKSQGQYEVACEHVHSCCVLLAKTDKFRVNGQWFTWIDYEKFHDLVASGKPFDS 600
           FSEALAAKSQG+YEVACEHVHSCCVLLAKTDKFR++GQWFTWIDYEKFH LVASGKPFDS
Sbjct: 541 FSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRIDGQWFTWIDYEKFHALVASGKPFDS 600

Query: 601 KDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHHRPKPMS 640
           KDYMAPTPSWAVYG+DEGGFDP QSRYRKERHHRPKP S
Sbjct: 601 KDYMAPTPSWAVYGADEGGFDPVQSRYRKERHHRPKPTS 639

BLAST of MS016821 vs. ExPASy TrEMBL
Match: A0A5A7VC83 (tRNA 4-demethylwyosine synthase (AdoMet-dependent) OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G005790 PE=3 SV=1)

HSP 1 Score: 1203.3 bits (3112), Expect = 0.0e+00
Identity = 586/641 (91.42%), Postives = 613/641 (95.63%), Query Frame = 0

Query: 1   MSMSSLSARLTLLALLSASTFYFFYKSRRLRKLKLSLNPTVCSRRAKLFFISETGTSKAL 60
           MS SS SA+LT+LALLSAST Y FYKSRRLRKLKLSLNPT+ SR+ KLFFIS+TGTSKAL
Sbjct: 1   MSFSSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTLSSRKPKLFFISQTGTSKAL 60

Query: 61  AHRLLNVLNSNELAFDLVDPEDYEPEDLFKESLVLFVASTWEDGGPPPHAKFFANWLSES 120
           AHRLLNVLNSN L FDLVDP+DYEPEDLFKE+LVLFVASTWEDGGPPPHAKF ANWLSES
Sbjct: 61  AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES 120

Query: 121 AEDFRVGSLLLSECKFGVFGVGSRAYGDTFNAVARGFSKQMRALGAKEILPVGEGDVDGG 180
           AEDFRVGSLLLS+CKF VFGVGSR YG+T+NAVAR FSK+M+ALGAKEILP+GEGDVDGG
Sbjct: 121 AEDFRVGSLLLSQCKFSVFGVGSRVYGETYNAVARDFSKRMKALGAKEILPIGEGDVDGG 180

Query: 181 DIDKCFDDWSQQVLKVLKVDAVGSGTDLYSAIAGDSDAESVED--DEEDADLGAEDIVDL 240
           DIDKCFDDWS+++LKV+KVDA G+G +L S IAGDSDA SVE+  +EED DLG EDIVDL
Sbjct: 181 DIDKCFDDWSRKLLKVVKVDAEGNGVELCSGIAGDSDAVSVEEEYEEEDDDLGEEDIVDL 240

Query: 241 EDIAGKAPSRKSTNVVETNGKLNGKKEMVTPVIRASLEKQGYKIIGTHSGVKICRWTKSQ 300
           EDIAGK PSRKSTNVVETNGKLNGKK MVTPVIRASLEKQGYKIIG+HSGVKICRWTKSQ
Sbjct: 241 EDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ 300

Query: 301 LRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVN 360
           LRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVN
Sbjct: 301 LRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVN 360

Query: 361 SAIDLHTKMIKQMKGVPGVTQEKLAEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRIS 420
           SAIDLHTKMIKQMKGVPGVTQEKL EGLSPRHCA SLVGEPIMYPEINTLVDELH+RRIS
Sbjct: 361 SAIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCAFSLVGEPIMYPEINTLVDELHQRRIS 420

Query: 421 TFLVTNAQFPDKIKSLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNDKQ 480
           TFLVTNAQFPDKIK LKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALN+KQ
Sbjct: 421 TFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNEKQ 480

Query: 481 QRTVYRLTLVKGWNIEDIDAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENVPWHSDV 540
           QRTVYRLTLVKGWN EDIDAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENVPWHSDV
Sbjct: 481 QRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENVPWHSDV 540

Query: 541 KSFSEALAAKSQGQYEVACEHVHSCCVLLAKTDKFRVNGQWFTWIDYEKFHDLVASGKPF 600
           KSFSEALAAKSQG+YEVACEHVHSCCVLLAKTDKFRV+GQWFTWIDYEKFHDLVASGKPF
Sbjct: 541 KSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHDLVASGKPF 600

Query: 601 DSKDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHHRPKPMS 640
           DSKDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHH+PKP S
Sbjct: 601 DSKDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHHKPKPTS 641

BLAST of MS016821 vs. ExPASy TrEMBL
Match: A0A1S3BAY2 (tRNA 4-demethylwyosine synthase (AdoMet-dependent) OS=Cucumis melo OX=3656 GN=LOC103487965 PE=3 SV=1)

HSP 1 Score: 1202.2 bits (3109), Expect = 0.0e+00
Identity = 585/641 (91.26%), Postives = 614/641 (95.79%), Query Frame = 0

Query: 1   MSMSSLSARLTLLALLSASTFYFFYKSRRLRKLKLSLNPTVCSRRAKLFFISETGTSKAL 60
           MS SS SA+LT+LALLSAST Y FYKSRRLRKLKLSLNPT+ SR+ KLFFIS+TGTSKAL
Sbjct: 1   MSFSSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTLSSRKPKLFFISQTGTSKAL 60

Query: 61  AHRLLNVLNSNELAFDLVDPEDYEPEDLFKESLVLFVASTWEDGGPPPHAKFFANWLSES 120
           AHRLLNVLNSN L FDLVDP+DYEPEDLFKE+LVLFVASTWEDGGPPPHAKF ANWLSES
Sbjct: 61  AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES 120

Query: 121 AEDFRVGSLLLSECKFGVFGVGSRAYGDTFNAVARGFSKQMRALGAKEILPVGEGDVDGG 180
           AEDFRVGSLLLS+CKF VFGVGSR YG+T+NAVAR FSK+M++LGAKEILP+GEGDVDGG
Sbjct: 121 AEDFRVGSLLLSQCKFSVFGVGSRVYGETYNAVARDFSKRMKSLGAKEILPIGEGDVDGG 180

Query: 181 DIDKCFDDWSQQVLKVLKVDAVGSGTDLYSAIAGDSDAESVED--DEEDADLGAEDIVDL 240
           DIDKCFDDWS+++LKV+KVDA G+G +L S IAGDSDA SVE+  +EED DLG EDIVDL
Sbjct: 181 DIDKCFDDWSRKLLKVVKVDAEGNGVELCSGIAGDSDAVSVEEEYEEEDDDLGEEDIVDL 240

Query: 241 EDIAGKAPSRKSTNVVETNGKLNGKKEMVTPVIRASLEKQGYKIIGTHSGVKICRWTKSQ 300
           EDIAGK PSRKSTNVVETNGKLNGKK MVTPVIRASLEKQGYKIIG+HSGVKICRWTKSQ
Sbjct: 241 EDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ 300

Query: 301 LRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVN 360
           LRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVN
Sbjct: 301 LRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVN 360

Query: 361 SAIDLHTKMIKQMKGVPGVTQEKLAEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRIS 420
           SAIDLHTKMIKQMKGVPGVTQEKL EGLSPRHCALSLVGEPIMYPEINTLVDELH+RRIS
Sbjct: 361 SAIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHQRRIS 420

Query: 421 TFLVTNAQFPDKIKSLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNDKQ 480
           TFLVTNAQFPDKIK LKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALN+KQ
Sbjct: 421 TFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNEKQ 480

Query: 481 QRTVYRLTLVKGWNIEDIDAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENVPWHSDV 540
           QRTVYRLTLVKGWN EDIDAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENVPWHSDV
Sbjct: 481 QRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENVPWHSDV 540

Query: 541 KSFSEALAAKSQGQYEVACEHVHSCCVLLAKTDKFRVNGQWFTWIDYEKFHDLVASGKPF 600
           KSFSEALAAKSQG+YEVACEHVHSCCVLLAKTDKFRV+GQWFTWIDYEKFHDLVASGKPF
Sbjct: 541 KSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHDLVASGKPF 600

Query: 601 DSKDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHHRPKPMS 640
           DSKDY+APTPSWAVYGSDEGGFDPDQSRYRKERHH+PKP S
Sbjct: 601 DSKDYIAPTPSWAVYGSDEGGFDPDQSRYRKERHHKPKPTS 641

BLAST of MS016821 vs. TAIR 10
Match: AT1G75200.1 (flavodoxin family protein / radical SAM domain-containing protein )

HSP 1 Score: 953.0 bits (2462), Expect = 1.2e-277
Identity = 469/642 (73.05%), Postives = 538/642 (83.80%), Query Frame = 0

Query: 4   SSLSARLTLLALLSASTFYFFYKSRRLRKLK-LSLNP--TVCSRRAKLFFISETGTSKAL 63
           SS+  RL  +ALLSA+TFY  +K RRL+ LK LSLNP  T+ + R K+FFIS+TGT+KAL
Sbjct: 5   SSVRVRLAFVALLSATTFYCIHKYRRLKHLKNLSLNPSSTLKASRGKIFFISQTGTAKAL 64

Query: 64  AHRLLNVLNSNELAFDLVDPEDYEPEDLFKESLVLFVASTWEDGGPPPHAKFFANWLSES 123
           A RL  +  SN++AFD+VDP  YEPEDL KE+LVLF+ASTW+ G PP + +F  NWL ES
Sbjct: 65  AQRLHELCASNDIAFDIVDPHSYEPEDLPKETLVLFIASTWDGGKPPKNGEFLVNWLGES 124

Query: 124 AEDFRVGSLLLSECKFGVFGVGSRAYGDTFNAVARGFSKQMRALGAKEILPVGEGDVDGG 183
           AEDFRVGSLLLS+CKF VFGVGSRAYG+++NAVA+  S +M  LG  E++PVGEGDVD G
Sbjct: 125 AEDFRVGSLLLSDCKFAVFGVGSRAYGESYNAVAKELSSRMIGLGGLEMIPVGEGDVDDG 184

Query: 184 DIDKCFDDWSQQVLKVLKVDAVGSGTDLYSAI-AGDSDAESVED-DEEDADLGAE-DIVD 243
           ++D+ F DW   V++VLK  +      +   I A + D E  +  DEED D  A+  IVD
Sbjct: 185 ELDRAFQDWCDGVIRVLKGGSAQETNGVSQQIGAVEDDLEYYDSTDEEDEDNDADGGIVD 244

Query: 244 LEDIAGKAPSRKSTNVVETNGKLNGKKEMVTPVIRASLEKQGYKIIGTHSGVKICRWTKS 303
           LEDIAGKAPS+++  V  T  K++GKKEMVTPVIRASL KQGYKIIG+HSGVKICRWTKS
Sbjct: 245 LEDIAGKAPSKRNGVVKVT--KVDGKKEMVTPVIRASLTKQGYKIIGSHSGVKICRWTKS 304

Query: 304 QLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIV 363
           QLRGRGGCYKHSFYGIESHRCME TPSLACANKCVFCWRHHTNPVGKSWQW+MD+P  IV
Sbjct: 305 QLRGRGGCYKHSFYGIESHRCMETTPSLACANKCVFCWRHHTNPVGKSWQWKMDEPSVIV 364

Query: 364 NSAIDLHTKMIKQMKGVPGVTQEKLAEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRI 423
             A+DLH  MIKQMKGVPGVT EKL EGL+PRHCALSLVGEPIMYPEIN LVDELH RRI
Sbjct: 365 KGALDLHKNMIKQMKGVPGVTPEKLQEGLNPRHCALSLVGEPIMYPEINALVDELHGRRI 424

Query: 424 STFLVTNAQFPDKIKSLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNDK 483
           STFLVTNAQFP+KI  +KP+TQLYVSVDAATKESLKAIDRPLF DFWERFIDSLKAL +K
Sbjct: 425 STFLVTNAQFPEKILMMKPITQLYVSVDAATKESLKAIDRPLFADFWERFIDSLKALQEK 484

Query: 484 QQRTVYRLTLVKGWNIEDIDAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENVPWHSD 543
           QQRTVYRLTLVKGWN E++DAY NLFSIG+PDF+EIKGVTYCGSS TSKLTMENVPWH+D
Sbjct: 485 QQRTVYRLTLVKGWNTEELDAYFNLFSIGKPDFIEIKGVTYCGSSATSKLTMENVPWHTD 544

Query: 544 VKSFSEALAAKSQGQYEVACEHVHSCCVLLAKTDKFRVNGQWFTWIDYEKFHDLVASGKP 603
           VK+FSEAL+ KS G+YEVACEH HSCCVLL +T+KF+V+G+WFTWIDYEKFHDLVASG+P
Sbjct: 545 VKAFSEALSLKSNGEYEVACEHAHSCCVLLGRTEKFKVDGKWFTWIDYEKFHDLVASGEP 604

Query: 604 FDSKDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHHRPKPMS 640
           F S DYMA TPSWAVYG+ EGGFDP Q RY+KER+H PKP +
Sbjct: 605 FTSTDYMAQTPSWAVYGAQEGGFDPGQLRYKKERNHPPKPQA 644