MS016819 (gene) Bitter gourd (TR) v1

BLAST of MS016819 vs. NCBI nr
Match: XP_022134260.1 (metalloendoproteinase 1-like [Momordica charantia])

HSP 1 Score: 416.8 bits (1070), Expect = 1.3e-112
Identity = 198/253 (78.26%), Postives = 217/253 (85.77%), Query Frame = 0

Query: 1   SPSPFHFPHEHLQGSRKGRHYIEGIHNLKLYLQYFGYLTNLPTYAHNTFDDALESALKIY 60
           SPSPFHFP +HLQG RKG   +EGIHN+KLYLQ+FGYLTN+PT+ HNTFDD LESALK Y
Sbjct: 31  SPSPFHFP-QHLQGPRKG-DTVEGIHNVKLYLQHFGYLTNVPTHTHNTFDDLLESALKTY 90

Query: 61  QKNYHLNATDILDVETLTLLSMPRCGHPDIVNPDYVIAPGRPKWPPTKYHLSYAFINDFP 120
           QKNYHLN T +LD ETLTLLS PRCGHPDI+NPDYVIAPGRPKWPPTKYHL+YAFI++FP
Sbjct: 91  QKNYHLNVTGVLDGETLTLLSTPRCGHPDIMNPDYVIAPGRPKWPPTKYHLTYAFIHNFP 150

Query: 121 PNFEEPVQCSLNTWTSVSNFTFSEATEATAD----FERGDHGDGVPFCGP-ILAHSFFPT 180
            NFE PV+CSLNTW+ VS FTFSE TEA AD    FERGDHGDG PF GP IL H+FFP 
Sbjct: 151 QNFEAPVRCSLNTWSGVSKFTFSETTEAIADVKISFERGDHGDGAPFYGPNILGHAFFPP 210

Query: 181 DGRLHFNVDQNWAWGAVAGSVDVQTVALHELGHILGLAHSQDECAIMYAYICPGTTKCMS 240
           DGRLH N DQNW WGAVA S DVQTV+LHELGHILGLAHSQDE A+MYAY  PGTTK +S
Sbjct: 211 DGRLHLNXDQNWVWGAVANSADVQTVSLHELGHILGLAHSQDEGAVMYAYTYPGTTKGLS 270

Query: 241 KDDIAGITDLYSS 249
           +DDIAGITDLYS+
Sbjct: 271 QDDIAGITDLYST 281

BLAST of MS016819 vs. NCBI nr
Match: XP_022134247.1 (metalloendoproteinase 3-MMP-like [Momordica charantia])

HSP 1 Score: 380.9 bits (977), Expect = 8.1e-102
Identity = 180/253 (71.15%), Postives = 209/253 (82.61%), Query Frame = 0

Query: 1   SPSPFHFPHEHLQGSRKGRHYIEGIHNLKLYLQYFGYLTNLPTYAHNTFDDALESALKIY 60
           +PSPFHFP +HLQGSRKG   +EGIHN+K YLQ+FGYLTN+ T+A NTFDD LE AL+ +
Sbjct: 29  TPSPFHFP-KHLQGSRKG-DTVEGIHNVKFYLQHFGYLTNISTHARNTFDDPLEYALRTF 88

Query: 61  QKNYHLNATDILDVETLTLLSMPRCGHPDIVNPDYVIAPGRPKWPPTKYHLSYAFINDFP 120
           QKNY LNAT ILDVETLTLLS PRCGHPDIV+P+Y + PGRPKWPPTKYHL+YAFI++FP
Sbjct: 89  QKNYRLNATGILDVETLTLLSTPRCGHPDIVHPNYALVPGRPKWPPTKYHLTYAFIHNFP 148

Query: 121 PNFEEPVQCSLNTWTSVSNFTFSEATEATAD----FERGDHGDGVPFCGP-ILAHSFFPT 180
           PNFE PV+CSL+TW+ VS FTFSE  EA +D    FERGDHGDG PF  P  LAH+F+PT
Sbjct: 149 PNFEAPVRCSLDTWSGVSKFTFSETIEAASDVKISFERGDHGDGCPFYSPNYLAHAFYPT 208

Query: 181 DGRLHFNVDQNWAWGAVAGSVDVQTVALHELGHILGLAHSQDECAIMYAYICPGTTKCMS 240
           DGRLH N+D NW WG+V  + DVQTV+LHELGHILGLAHS DE A+MYAYI  G  K ++
Sbjct: 209 DGRLHLNMDTNWVWGSVDSASDVQTVSLHELGHILGLAHSLDEGAVMYAYIPHGVVKSVT 268

Query: 241 KDDIAGITDLYSS 249
           +DDIAGITDLY S
Sbjct: 269 QDDIAGITDLYGS 279

BLAST of MS016819 vs. NCBI nr
Match: XP_022134250.1 (metalloendoproteinase 3-MMP-like [Momordica charantia])

HSP 1 Score: 379.0 bits (972), Expect = 3.1e-101
Identity = 179/253 (70.75%), Postives = 209/253 (82.61%), Query Frame = 0

Query: 1   SPSPFHFPHEHLQGSRKGRHYIEGIHNLKLYLQYFGYLTNLPTYAHNTFDDALESALKIY 60
           +PSPFHFP +HLQGSRKG   +EGIHN+K YLQ+FGYLTN+ T+A NTFDD LE AL+ +
Sbjct: 29  TPSPFHFP-KHLQGSRKG-DTVEGIHNVKFYLQHFGYLTNISTHARNTFDDPLEYALRTF 88

Query: 61  QKNYHLNATDILDVETLTLLSMPRCGHPDIVNPDYVIAPGRPKWPPTKYHLSYAFINDFP 120
           QKNY LNAT ILDVETLTLLS PRCGHP+IV+P+Y + PGRPKWPPTKYHL+YAFI++FP
Sbjct: 89  QKNYRLNATGILDVETLTLLSTPRCGHPNIVHPNYALVPGRPKWPPTKYHLTYAFIHNFP 148

Query: 121 PNFEEPVQCSLNTWTSVSNFTFSEATEATAD----FERGDHGDGVPFCGP-ILAHSFFPT 180
           PNFE PV+CSL+TW+ VS FTFSE  EA +D    FERGDHGDG PF  P  LAH+F+PT
Sbjct: 149 PNFEAPVRCSLDTWSGVSKFTFSETIEAASDVKISFERGDHGDGCPFYSPNYLAHAFYPT 208

Query: 181 DGRLHFNVDQNWAWGAVAGSVDVQTVALHELGHILGLAHSQDECAIMYAYICPGTTKCMS 240
           DGRLH N+D NW WG+V  + DVQTV+LHELGHILGLAHS DE A+MYAYI  G  K ++
Sbjct: 209 DGRLHLNMDTNWVWGSVDSASDVQTVSLHELGHILGLAHSLDEGAVMYAYIPHGVVKSVT 268

Query: 241 KDDIAGITDLYSS 249
           +DDIAGITDLY S
Sbjct: 269 QDDIAGITDLYGS 279

BLAST of MS016819 vs. NCBI nr
Match: XP_022133615.1 (metalloendoproteinase 1-like [Momordica charantia])

HSP 1 Score: 305.4 bits (781), Expect = 4.3e-79
Identity = 143/195 (73.33%), Postives = 159/195 (81.54%), Query Frame = 0

Query: 59  IYQKNYHLNATDILDVETLTLLSMPRCGHPDIVNPDYVIAPGRPKWPPTKYHLSYAFIND 118
           +YQKNYHLNA  +LD ETLTLLS PRCGHPDIV+ DY I PGRPKW PTKYHL+YAFI++
Sbjct: 1   MYQKNYHLNAIGVLDAETLTLLSTPRCGHPDIVHLDYAIVPGRPKWLPTKYHLTYAFIHN 60

Query: 119 FPPNFEEPVQCSLNTWTSVSNFTFSEATEATAD----FERGDHGDGVPFCGP-ILAHSFF 178
           FPPNFE PV+CSLN W+ VS FTFSE TEA AD    FERGDHGDG PF  P ILAH+F+
Sbjct: 61  FPPNFEAPVRCSLNAWSGVSKFTFSETTEAAADVKISFERGDHGDGYPFYNPNILAHAFY 120

Query: 179 PTDGRLHFNVDQNWAWGAVAGSVDVQTVALHELGHILGLAHSQDECAIMYAYICPGTTKC 238
           PTDGRLH NVDQNW WG+VA + DVQTV+LHELGH LGLAHS DE A+MYAYI  G  K 
Sbjct: 121 PTDGRLHLNVDQNWVWGSVAFASDVQTVSLHELGHTLGLAHSLDEGAVMYAYIPQGVVKS 180

Query: 239 MSKDDIAGITDLYSS 249
           ++ DDIAGITDLY S
Sbjct: 181 LTPDDIAGITDLYGS 195

BLAST of MS016819 vs. NCBI nr
Match: XP_022134769.1 (metalloendoproteinase 1-like [Momordica charantia])

HSP 1 Score: 268.1 bits (684), Expect = 7.7e-68
Identity = 142/246 (57.72%), Postives = 153/246 (62.20%), Query Frame = 0

Query: 3   SPFHFPHEHLQGSRKGRHYIEGIHNLKLYLQYFGYLTNLPTYAHNTFDDALESALKIYQK 62
           SPF FPHEHLQ SRKG   +EGIHN+KLYLQ+F YLTNLP +AHNTFDD LESALK YQK
Sbjct: 36  SPFSFPHEHLQDSRKG-DTVEGIHNVKLYLQHFDYLTNLPAHAHNTFDDPLESALKTYQK 95

Query: 63  NYHLNATDILDVETLTLLSMPRCGHPDIVNPDYVIAPGRPKWPPTKYHLSYAFINDFPPN 122
           NYHLNAT   +V+                                               
Sbjct: 96  NYHLNATATANVK----------------------------------------------- 155

Query: 123 FEEPVQCSLNTWTSVSNFTFSEATEATADFERGDHGDGVPFCGPILAHSFFPTDGRLHFN 182
                                        FE+GDHGD VPFC PILAHSFF TD RLHFN
Sbjct: 156 ---------------------------IPFEKGDHGDVVPFCTPILAHSFFSTDERLHFN 206

Query: 183 VDQNWAWGAVAGSVDVQTVALHELGHILGLAHSQDECAIMYAYICPGTTKCMSKDDIAGI 242
           VDQNWAWGAVAG+VDVQTVALHELGHILGLAH+QDECAIMYAYICPGTTKCM++DDIA I
Sbjct: 216 VDQNWAWGAVAGAVDVQTVALHELGHILGLAHNQDECAIMYAYICPGTTKCMTQDDIARI 206

Query: 243 TDLYSS 249
           TDLYSS
Sbjct: 276 TDLYSS 206

BLAST of MS016819 vs. ExPASy Swiss-Prot
Match: P29136 (Metalloendoproteinase 1 OS=Glycine max OX=3847 PE=1 SV=2)

HSP 1 Score: 175.3 bits (443), Expect = 8.9e-43
Identity = 104/256 (40.62%), Postives = 139/256 (54.30%), Query Frame = 0

Query: 18  GRHYIEGIHNLKLYLQYFGYLTNLPTYAHNTFDDALESALKIYQKNYHLNATDILDVETL 77
           G++Y +G+ N+K Y  + GY+ N P +  N FDD L SA+K YQKNY+LN T   D+ TL
Sbjct: 48  GQNY-KGLSNVKNYFHHLGYIPNAPHFDDN-FDDTLVSAIKTYQKNYNLNVTGKFDINTL 107

Query: 78  TLLSMPRCGHPDIV-----------NPDYVIAPGRPKWPPTKYHLSYAFIND--FPPNFE 137
             +  PRCG PDI+             DY      P+W      L+YAF  +      F+
Sbjct: 108 KQIMTPRCGVPDIIINTNKTTSFGMISDYTFFKDMPRWQAGTTQLTYAFSPEPRLDDTFK 167

Query: 138 EPVQCSLNTWTSVSNFTFSEATE-ATAD----FERGDHGDGVPFCGP--ILAHSFFPTDG 197
             +  + + WT V N  F E T   TA+    F   +HGD  PF GP  IL H+F PTDG
Sbjct: 168 SAIARAFSKWTPVVNIAFQETTSYETANIKILFASKNHGDPYPFDGPGGILGHAFAPTDG 227

Query: 198 RLHFNVDQNWAWGA------VAGSVDVQTVALHELGHILGLAHSQDECAIMYAYICPGTT 247
           R HF+ D+ W          V  + D+++VA+HE+GH+LGL HS D  AIMY  I P T 
Sbjct: 228 RCHFDADEYWVASGDVTKSPVTSAFDLESVAVHEIGHLLGLGHSSDLRAIMYPSIPPRTR 287

BLAST of MS016819 vs. ExPASy Swiss-Prot
Match: O04529 (Metalloendoproteinase 2-MMP OS=Arabidopsis thaliana OX=3702 GN=2MMP PE=1 SV=1)

HSP 1 Score: 167.5 bits (423), Expect = 1.8e-40
Identity = 97/272 (35.66%), Postives = 148/272 (54.41%), Query Frame = 0

Query: 22  IEGIHNLKLYLQYFGYLTNLPTYAHN---TFDDALESALKIYQKNYHLNATDILDVETLT 81
           ++G++ +K Y Q FGY+    T++ N    FDD L++A+++YQ N++LN T  LD  T+ 
Sbjct: 56  VDGLYRIKKYFQRFGYIPE--TFSGNFTDDFDDILKAAVELYQTNFNLNVTGELDALTIQ 115

Query: 82  LLSMPRCGHPDIVN-------------------------PDYVIAPGRPKWPPTKYHLSY 141
            + +PRCG+PD+VN                           Y + PG P+WP  +  L+Y
Sbjct: 116 HIVIPRCGNPDVVNGTSLMHGGRRKTFEVNFSRTHLHAVKRYTLFPGEPRWPRNRRDLTY 175

Query: 142 AF--INDFPPNFEEPVQCSLNTWTSVS--NFTFSEA---TEATADFERGDHGDGVPFCGP 201
           AF   N      +     +   W+ V+  NFT SE+   ++ T  F  GDHGDG PF G 
Sbjct: 176 AFDPKNPLTEEVKSVFSRAFGRWSDVTALNFTLSESFSTSDITIGFYTGDHGDGEPFDGV 235

Query: 202 I--LAHSFFPTDGRLHFNVDQNW-------AWGAVAGSVDVQTVALHELGHILGLAHSQD 249
           +  LAH+F P  G+ H + D+NW       ++ +V  +VD+++VA+HE+GH+LGL HS  
Sbjct: 236 LGTLAHAFSPPSGKFHLDADENWVVSGDLDSFLSVTAAVDLESVAVHEIGHLLGLGHSSV 295

BLAST of MS016819 vs. ExPASy Swiss-Prot
Match: Q5XF51 (Metalloendoproteinase 3-MMP OS=Arabidopsis thaliana OX=3702 GN=3MMP PE=1 SV=1)

HSP 1 Score: 164.5 bits (415), Expect = 1.6e-39
Identity = 97/281 (34.52%), Postives = 148/281 (52.67%), Query Frame = 0

Query: 14  GSRKGRHYIEGIHNLKLYLQYFGYL--TNLPTYAHNTFDDALESALKIYQKNYHLNATDI 73
           G   G+ Y +G++ LK Y Q+FGY+  TNL     + FDD L++A+++YQ+N+ LN T +
Sbjct: 51  GCHAGKKY-DGLYMLKQYFQHFGYITETNLSGNFTDDFDDILKNAVEMYQRNFQLNVTGV 110

Query: 74  LDVETLTLLSMPRCGHPDIVN--------------------------PDYVIAPGRPKWP 133
           LD  TL  + +PRCG+PD+VN                            Y   PG P+WP
Sbjct: 111 LDELTLKHVVIPRCGNPDVVNGTSTMHSGRKTFEVSFAGRGQRFHAVKHYSFFPGEPRWP 170

Query: 134 PTKYHLSYAF--INDFPPNFEEPVQCSLNTWTSVSNFTFS-----EATEATADFERGDHG 193
             +  L+YAF   N      +     +   W  V+  TF+       ++ +  F  G+HG
Sbjct: 171 RNRRDLTYAFDPRNALTEEVKSVFSRAFTRWEEVTPLTFTRVERFSTSDISIGFYSGEHG 230

Query: 194 DGVPFCGPI--LAHSFFPTDGRLHFNVDQNW--------AWGAVAGSVDVQTVALHELGH 249
           DG PF GP+  LAH+F P  G  H + ++NW         + +V+ +VD+++VA+HE+GH
Sbjct: 231 DGEPFDGPMRTLAHAFSPPTGHFHLDGEENWIVSGEGGDGFISVSEAVDLESVAVHEIGH 290

BLAST of MS016819 vs. ExPASy Swiss-Prot
Match: Q9ZUJ5 (Metalloendoproteinase 5-MMP OS=Arabidopsis thaliana OX=3702 GN=5MMP PE=1 SV=1)

HSP 1 Score: 159.1 bits (401), Expect = 6.6e-38
Identity = 97/258 (37.60%), Postives = 134/258 (51.94%), Query Frame = 0

Query: 22  IEGIHNLKLYLQYFGYLTNLPTYAHNTFDDALESALKIYQKNYHLNATDILDVETLTLLS 81
           I G+  LK Y + FGY+T       + FDD L+SA+  YQKN++L  T  LD  TL  + 
Sbjct: 57  INGLSKLKQYFRRFGYITTTGN-CTDDFDDVLQSAINTYQKNFNLKVTGKLDSSTLRQIV 116

Query: 82  MPRCGHPDIVN--------------PDYVIAPGRPKWPPTKYHLSYAFI--NDFPPNFEE 141
            PRCG+PD+++                Y   PG+P+WP  K  L+YAF   N+     + 
Sbjct: 117 KPRCGNPDLIDGVSEMNGGKILRTTEKYSFFPGKPRWPKRKRDLTYAFAPQNNLTDEVKR 176

Query: 142 PVQCSLNTWTSVS--NFTFSEA---TEATADFERGDHGDGVPFCGPI--LAHSFFPTDGR 201
               +   W  V+  NFT SE+    +    F  G+HGDG PF G +  LAH+  P  G 
Sbjct: 177 VFSRAFTRWAEVTPLNFTRSESILRADIVIGFFSGEHGDGEPFDGAMGTLAHASSPPTGM 236

Query: 202 LHFNVDQNWAWG---------AVAGSVDVQTVALHELGHILGLAHSQDECAIMYAYICPG 247
           LH + D++W             V   VD+++VA+HE+GH+LGL HS  E AIM+  I  G
Sbjct: 237 LHLDGDEDWLISNGEISRRILPVTTVVDLESVAVHEIGHLLGLGHSSVEDAIMFPAISGG 296

BLAST of MS016819 vs. ExPASy Swiss-Prot
Match: O23507 (Metalloendoproteinase 1-MMP OS=Arabidopsis thaliana OX=3702 GN=1MMP PE=1 SV=1)

HSP 1 Score: 156.4 bits (394), Expect = 4.3e-37
Identity = 97/255 (38.04%), Postives = 140/255 (54.90%), Query Frame = 0

Query: 21  YIEGIHNLKLYLQYFGYLTNLPTYAHNTFDDALESALKIYQKNYHLNATDILDVETLTLL 80
           ++ G+  LK YL  FGY+ +      + FD  LESA+ +YQ+N  L  T  LD  T+TL+
Sbjct: 66  HVSGVSELKRYLHRFGYVNDGSEIFSDVFDGPLESAISLYQENLGLPITGRLDTSTVTLM 125

Query: 81  SMPRCGHPD---IVNPD-------YVIAPGRPKWPPTKYHLSYAFINDFPPNF--EEPVQ 140
           S+PRCG  D    +N D       Y    G+PKW   +  L+YA       ++   E V+
Sbjct: 126 SLPRCGVSDTHMTINNDFLHTTAHYTYFNGKPKW--NRDTLTYAISKTHKLDYLTSEDVK 185

Query: 141 C----SLNTWTSVSNFTFSEATE-ATAD----FERGDHGDGVPFCGPI--LAHSFFPTDG 200
                + + W+SV   +F E  +  TAD    F  GDHGDG+PF G +  LAH+F P +G
Sbjct: 186 TVFRRAFSQWSSVIPVSFEEVDDFTTADLKIGFYAGDHGDGLPFDGVLGTLAHAFAPENG 245

Query: 201 RLHFNVDQNW-----AWGAVAGSVDVQTVALHELGHILGLAHSQDECAIMYAYICPGTTK 247
           RLH +  + W       G+   +VD+++VA HE+GH+LGL HS  E A+MY  + P T K
Sbjct: 246 RLHLDAAETWIVDDDLKGSSEVAVDLESVATHEIGHLLGLGHSSQESAVMYPSLRPRTKK 305

BLAST of MS016819 vs. ExPASy TrEMBL
Match: A0A6J1BXS7 (metalloendoproteinase 1-like OS=Momordica charantia OX=3673 GN=LOC111006562 PE=3 SV=1)

HSP 1 Score: 416.8 bits (1070), Expect = 6.5e-113
Identity = 198/253 (78.26%), Postives = 217/253 (85.77%), Query Frame = 0

Query: 1   SPSPFHFPHEHLQGSRKGRHYIEGIHNLKLYLQYFGYLTNLPTYAHNTFDDALESALKIY 60
           SPSPFHFP +HLQG RKG   +EGIHN+KLYLQ+FGYLTN+PT+ HNTFDD LESALK Y
Sbjct: 31  SPSPFHFP-QHLQGPRKG-DTVEGIHNVKLYLQHFGYLTNVPTHTHNTFDDLLESALKTY 90

Query: 61  QKNYHLNATDILDVETLTLLSMPRCGHPDIVNPDYVIAPGRPKWPPTKYHLSYAFINDFP 120
           QKNYHLN T +LD ETLTLLS PRCGHPDI+NPDYVIAPGRPKWPPTKYHL+YAFI++FP
Sbjct: 91  QKNYHLNVTGVLDGETLTLLSTPRCGHPDIMNPDYVIAPGRPKWPPTKYHLTYAFIHNFP 150

Query: 121 PNFEEPVQCSLNTWTSVSNFTFSEATEATAD----FERGDHGDGVPFCGP-ILAHSFFPT 180
            NFE PV+CSLNTW+ VS FTFSE TEA AD    FERGDHGDG PF GP IL H+FFP 
Sbjct: 151 QNFEAPVRCSLNTWSGVSKFTFSETTEAIADVKISFERGDHGDGAPFYGPNILGHAFFPP 210

Query: 181 DGRLHFNVDQNWAWGAVAGSVDVQTVALHELGHILGLAHSQDECAIMYAYICPGTTKCMS 240
           DGRLH N DQNW WGAVA S DVQTV+LHELGHILGLAHSQDE A+MYAY  PGTTK +S
Sbjct: 211 DGRLHLNXDQNWVWGAVANSADVQTVSLHELGHILGLAHSQDEGAVMYAYTYPGTTKGLS 270

Query: 241 KDDIAGITDLYSS 249
           +DDIAGITDLYS+
Sbjct: 271 QDDIAGITDLYST 281

BLAST of MS016819 vs. ExPASy TrEMBL
Match: A0A6J1BZ43 (metalloendoproteinase 3-MMP-like OS=Momordica charantia OX=3673 GN=LOC111006549 PE=3 SV=1)

HSP 1 Score: 380.9 bits (977), Expect = 3.9e-102
Identity = 180/253 (71.15%), Postives = 209/253 (82.61%), Query Frame = 0

Query: 1   SPSPFHFPHEHLQGSRKGRHYIEGIHNLKLYLQYFGYLTNLPTYAHNTFDDALESALKIY 60
           +PSPFHFP +HLQGSRKG   +EGIHN+K YLQ+FGYLTN+ T+A NTFDD LE AL+ +
Sbjct: 29  TPSPFHFP-KHLQGSRKG-DTVEGIHNVKFYLQHFGYLTNISTHARNTFDDPLEYALRTF 88

Query: 61  QKNYHLNATDILDVETLTLLSMPRCGHPDIVNPDYVIAPGRPKWPPTKYHLSYAFINDFP 120
           QKNY LNAT ILDVETLTLLS PRCGHPDIV+P+Y + PGRPKWPPTKYHL+YAFI++FP
Sbjct: 89  QKNYRLNATGILDVETLTLLSTPRCGHPDIVHPNYALVPGRPKWPPTKYHLTYAFIHNFP 148

Query: 121 PNFEEPVQCSLNTWTSVSNFTFSEATEATAD----FERGDHGDGVPFCGP-ILAHSFFPT 180
           PNFE PV+CSL+TW+ VS FTFSE  EA +D    FERGDHGDG PF  P  LAH+F+PT
Sbjct: 149 PNFEAPVRCSLDTWSGVSKFTFSETIEAASDVKISFERGDHGDGCPFYSPNYLAHAFYPT 208

Query: 181 DGRLHFNVDQNWAWGAVAGSVDVQTVALHELGHILGLAHSQDECAIMYAYICPGTTKCMS 240
           DGRLH N+D NW WG+V  + DVQTV+LHELGHILGLAHS DE A+MYAYI  G  K ++
Sbjct: 209 DGRLHLNMDTNWVWGSVDSASDVQTVSLHELGHILGLAHSLDEGAVMYAYIPHGVVKSVT 268

Query: 241 KDDIAGITDLYSS 249
           +DDIAGITDLY S
Sbjct: 269 QDDIAGITDLYGS 279

BLAST of MS016819 vs. ExPASy TrEMBL
Match: A0A6J1BXR0 (metalloendoproteinase 3-MMP-like OS=Momordica charantia OX=3673 GN=LOC111006552 PE=3 SV=1)

HSP 1 Score: 379.0 bits (972), Expect = 1.5e-101
Identity = 179/253 (70.75%), Postives = 209/253 (82.61%), Query Frame = 0

Query: 1   SPSPFHFPHEHLQGSRKGRHYIEGIHNLKLYLQYFGYLTNLPTYAHNTFDDALESALKIY 60
           +PSPFHFP +HLQGSRKG   +EGIHN+K YLQ+FGYLTN+ T+A NTFDD LE AL+ +
Sbjct: 29  TPSPFHFP-KHLQGSRKG-DTVEGIHNVKFYLQHFGYLTNISTHARNTFDDPLEYALRTF 88

Query: 61  QKNYHLNATDILDVETLTLLSMPRCGHPDIVNPDYVIAPGRPKWPPTKYHLSYAFINDFP 120
           QKNY LNAT ILDVETLTLLS PRCGHP+IV+P+Y + PGRPKWPPTKYHL+YAFI++FP
Sbjct: 89  QKNYRLNATGILDVETLTLLSTPRCGHPNIVHPNYALVPGRPKWPPTKYHLTYAFIHNFP 148

Query: 121 PNFEEPVQCSLNTWTSVSNFTFSEATEATAD----FERGDHGDGVPFCGP-ILAHSFFPT 180
           PNFE PV+CSL+TW+ VS FTFSE  EA +D    FERGDHGDG PF  P  LAH+F+PT
Sbjct: 149 PNFEAPVRCSLDTWSGVSKFTFSETIEAASDVKISFERGDHGDGCPFYSPNYLAHAFYPT 208

Query: 181 DGRLHFNVDQNWAWGAVAGSVDVQTVALHELGHILGLAHSQDECAIMYAYICPGTTKCMS 240
           DGRLH N+D NW WG+V  + DVQTV+LHELGHILGLAHS DE A+MYAYI  G  K ++
Sbjct: 209 DGRLHLNMDTNWVWGSVDSASDVQTVSLHELGHILGLAHSLDEGAVMYAYIPHGVVKSVT 268

Query: 241 KDDIAGITDLYSS 249
           +DDIAGITDLY S
Sbjct: 269 QDDIAGITDLYGS 279

BLAST of MS016819 vs. ExPASy TrEMBL
Match: A0A6J1BVR8 (metalloendoproteinase 1-like OS=Momordica charantia OX=3673 GN=LOC111006153 PE=4 SV=1)

HSP 1 Score: 305.4 bits (781), Expect = 2.1e-79
Identity = 143/195 (73.33%), Postives = 159/195 (81.54%), Query Frame = 0

Query: 59  IYQKNYHLNATDILDVETLTLLSMPRCGHPDIVNPDYVIAPGRPKWPPTKYHLSYAFIND 118
           +YQKNYHLNA  +LD ETLTLLS PRCGHPDIV+ DY I PGRPKW PTKYHL+YAFI++
Sbjct: 1   MYQKNYHLNAIGVLDAETLTLLSTPRCGHPDIVHLDYAIVPGRPKWLPTKYHLTYAFIHN 60

Query: 119 FPPNFEEPVQCSLNTWTSVSNFTFSEATEATAD----FERGDHGDGVPFCGP-ILAHSFF 178
           FPPNFE PV+CSLN W+ VS FTFSE TEA AD    FERGDHGDG PF  P ILAH+F+
Sbjct: 61  FPPNFEAPVRCSLNAWSGVSKFTFSETTEAAADVKISFERGDHGDGYPFYNPNILAHAFY 120

Query: 179 PTDGRLHFNVDQNWAWGAVAGSVDVQTVALHELGHILGLAHSQDECAIMYAYICPGTTKC 238
           PTDGRLH NVDQNW WG+VA + DVQTV+LHELGH LGLAHS DE A+MYAYI  G  K 
Sbjct: 121 PTDGRLHLNVDQNWVWGSVAFASDVQTVSLHELGHTLGLAHSLDEGAVMYAYIPQGVVKS 180

Query: 239 MSKDDIAGITDLYSS 249
           ++ DDIAGITDLY S
Sbjct: 181 LTPDDIAGITDLYGS 195

BLAST of MS016819 vs. ExPASy TrEMBL
Match: A0A6J1BZQ4 (metalloendoproteinase 1-like OS=Momordica charantia OX=3673 GN=LOC111006960 PE=3 SV=1)

HSP 1 Score: 268.1 bits (684), Expect = 3.7e-68
Identity = 142/246 (57.72%), Postives = 153/246 (62.20%), Query Frame = 0

Query: 3   SPFHFPHEHLQGSRKGRHYIEGIHNLKLYLQYFGYLTNLPTYAHNTFDDALESALKIYQK 62
           SPF FPHEHLQ SRKG   +EGIHN+KLYLQ+F YLTNLP +AHNTFDD LESALK YQK
Sbjct: 36  SPFSFPHEHLQDSRKG-DTVEGIHNVKLYLQHFDYLTNLPAHAHNTFDDPLESALKTYQK 95

Query: 63  NYHLNATDILDVETLTLLSMPRCGHPDIVNPDYVIAPGRPKWPPTKYHLSYAFINDFPPN 122
           NYHLNAT   +V+                                               
Sbjct: 96  NYHLNATATANVK----------------------------------------------- 155

Query: 123 FEEPVQCSLNTWTSVSNFTFSEATEATADFERGDHGDGVPFCGPILAHSFFPTDGRLHFN 182
                                        FE+GDHGD VPFC PILAHSFF TD RLHFN
Sbjct: 156 ---------------------------IPFEKGDHGDVVPFCTPILAHSFFSTDERLHFN 206

Query: 183 VDQNWAWGAVAGSVDVQTVALHELGHILGLAHSQDECAIMYAYICPGTTKCMSKDDIAGI 242
           VDQNWAWGAVAG+VDVQTVALHELGHILGLAH+QDECAIMYAYICPGTTKCM++DDIA I
Sbjct: 216 VDQNWAWGAVAGAVDVQTVALHELGHILGLAHNQDECAIMYAYICPGTTKCMTQDDIARI 206

Query: 243 TDLYSS 249
           TDLYSS
Sbjct: 276 TDLYSS 206

BLAST of MS016819 vs. TAIR 10
Match: AT1G70170.1 (matrix metalloproteinase )

HSP 1 Score: 167.5 bits (423), Expect = 1.3e-41
Identity = 97/272 (35.66%), Postives = 148/272 (54.41%), Query Frame = 0

Query: 22  IEGIHNLKLYLQYFGYLTNLPTYAHN---TFDDALESALKIYQKNYHLNATDILDVETLT 81
           ++G++ +K Y Q FGY+    T++ N    FDD L++A+++YQ N++LN T  LD  T+ 
Sbjct: 56  VDGLYRIKKYFQRFGYIPE--TFSGNFTDDFDDILKAAVELYQTNFNLNVTGELDALTIQ 115

Query: 82  LLSMPRCGHPDIVN-------------------------PDYVIAPGRPKWPPTKYHLSY 141
            + +PRCG+PD+VN                           Y + PG P+WP  +  L+Y
Sbjct: 116 HIVIPRCGNPDVVNGTSLMHGGRRKTFEVNFSRTHLHAVKRYTLFPGEPRWPRNRRDLTY 175

Query: 142 AF--INDFPPNFEEPVQCSLNTWTSVS--NFTFSEA---TEATADFERGDHGDGVPFCGP 201
           AF   N      +     +   W+ V+  NFT SE+   ++ T  F  GDHGDG PF G 
Sbjct: 176 AFDPKNPLTEEVKSVFSRAFGRWSDVTALNFTLSESFSTSDITIGFYTGDHGDGEPFDGV 235

Query: 202 I--LAHSFFPTDGRLHFNVDQNW-------AWGAVAGSVDVQTVALHELGHILGLAHSQD 249
           +  LAH+F P  G+ H + D+NW       ++ +V  +VD+++VA+HE+GH+LGL HS  
Sbjct: 236 LGTLAHAFSPPSGKFHLDADENWVVSGDLDSFLSVTAAVDLESVAVHEIGHLLGLGHSSV 295

BLAST of MS016819 vs. TAIR 10
Match: AT1G24140.1 (Matrixin family protein )

HSP 1 Score: 164.5 bits (415), Expect = 1.1e-40
Identity = 97/281 (34.52%), Postives = 148/281 (52.67%), Query Frame = 0

Query: 14  GSRKGRHYIEGIHNLKLYLQYFGYL--TNLPTYAHNTFDDALESALKIYQKNYHLNATDI 73
           G   G+ Y +G++ LK Y Q+FGY+  TNL     + FDD L++A+++YQ+N+ LN T +
Sbjct: 51  GCHAGKKY-DGLYMLKQYFQHFGYITETNLSGNFTDDFDDILKNAVEMYQRNFQLNVTGV 110

Query: 74  LDVETLTLLSMPRCGHPDIVN--------------------------PDYVIAPGRPKWP 133
           LD  TL  + +PRCG+PD+VN                            Y   PG P+WP
Sbjct: 111 LDELTLKHVVIPRCGNPDVVNGTSTMHSGRKTFEVSFAGRGQRFHAVKHYSFFPGEPRWP 170

Query: 134 PTKYHLSYAF--INDFPPNFEEPVQCSLNTWTSVSNFTFS-----EATEATADFERGDHG 193
             +  L+YAF   N      +     +   W  V+  TF+       ++ +  F  G+HG
Sbjct: 171 RNRRDLTYAFDPRNALTEEVKSVFSRAFTRWEEVTPLTFTRVERFSTSDISIGFYSGEHG 230

Query: 194 DGVPFCGPI--LAHSFFPTDGRLHFNVDQNW--------AWGAVAGSVDVQTVALHELGH 249
           DG PF GP+  LAH+F P  G  H + ++NW         + +V+ +VD+++VA+HE+GH
Sbjct: 231 DGEPFDGPMRTLAHAFSPPTGHFHLDGEENWIVSGEGGDGFISVSEAVDLESVAVHEIGH 290

BLAST of MS016819 vs. TAIR 10
Match: AT1G59970.1 (Matrixin family protein )

HSP 1 Score: 159.1 bits (401), Expect = 4.7e-39
Identity = 97/258 (37.60%), Postives = 134/258 (51.94%), Query Frame = 0

Query: 22  IEGIHNLKLYLQYFGYLTNLPTYAHNTFDDALESALKIYQKNYHLNATDILDVETLTLLS 81
           I G+  LK Y + FGY+T       + FDD L+SA+  YQKN++L  T  LD  TL  + 
Sbjct: 57  INGLSKLKQYFRRFGYITTTGN-CTDDFDDVLQSAINTYQKNFNLKVTGKLDSSTLRQIV 116

Query: 82  MPRCGHPDIVN--------------PDYVIAPGRPKWPPTKYHLSYAFI--NDFPPNFEE 141
            PRCG+PD+++                Y   PG+P+WP  K  L+YAF   N+     + 
Sbjct: 117 KPRCGNPDLIDGVSEMNGGKILRTTEKYSFFPGKPRWPKRKRDLTYAFAPQNNLTDEVKR 176

Query: 142 PVQCSLNTWTSVS--NFTFSEA---TEATADFERGDHGDGVPFCGPI--LAHSFFPTDGR 201
               +   W  V+  NFT SE+    +    F  G+HGDG PF G +  LAH+  P  G 
Sbjct: 177 VFSRAFTRWAEVTPLNFTRSESILRADIVIGFFSGEHGDGEPFDGAMGTLAHASSPPTGM 236

Query: 202 LHFNVDQNWAWG---------AVAGSVDVQTVALHELGHILGLAHSQDECAIMYAYICPG 247
           LH + D++W             V   VD+++VA+HE+GH+LGL HS  E AIM+  I  G
Sbjct: 237 LHLDGDEDWLISNGEISRRILPVTTVVDLESVAVHEIGHLLGLGHSSVEDAIMFPAISGG 296

BLAST of MS016819 vs. TAIR 10
Match: AT4G16640.1 (Matrixin family protein )

HSP 1 Score: 156.4 bits (394), Expect = 3.0e-38
Identity = 97/255 (38.04%), Postives = 140/255 (54.90%), Query Frame = 0

Query: 21  YIEGIHNLKLYLQYFGYLTNLPTYAHNTFDDALESALKIYQKNYHLNATDILDVETLTLL 80
           ++ G+  LK YL  FGY+ +      + FD  LESA+ +YQ+N  L  T  LD  T+TL+
Sbjct: 66  HVSGVSELKRYLHRFGYVNDGSEIFSDVFDGPLESAISLYQENLGLPITGRLDTSTVTLM 125

Query: 81  SMPRCGHPD---IVNPD-------YVIAPGRPKWPPTKYHLSYAFINDFPPNF--EEPVQ 140
           S+PRCG  D    +N D       Y    G+PKW   +  L+YA       ++   E V+
Sbjct: 126 SLPRCGVSDTHMTINNDFLHTTAHYTYFNGKPKW--NRDTLTYAISKTHKLDYLTSEDVK 185

Query: 141 C----SLNTWTSVSNFTFSEATE-ATAD----FERGDHGDGVPFCGPI--LAHSFFPTDG 200
                + + W+SV   +F E  +  TAD    F  GDHGDG+PF G +  LAH+F P +G
Sbjct: 186 TVFRRAFSQWSSVIPVSFEEVDDFTTADLKIGFYAGDHGDGLPFDGVLGTLAHAFAPENG 245

Query: 201 RLHFNVDQNW-----AWGAVAGSVDVQTVALHELGHILGLAHSQDECAIMYAYICPGTTK 247
           RLH +  + W       G+   +VD+++VA HE+GH+LGL HS  E A+MY  + P T K
Sbjct: 246 RLHLDAAETWIVDDDLKGSSEVAVDLESVATHEIGHLLGLGHSSQESAVMYPSLRPRTKK 305

BLAST of MS016819 vs. TAIR 10
Match: AT2G45040.1 (Matrixin family protein )

HSP 1 Score: 137.1 bits (344), Expect = 1.9e-32
Identity = 91/252 (36.11%), Postives = 129/252 (51.19%), Query Frame = 0

Query: 25  IHNLKLYLQYFGYLTNLPTYAHNTFDDALESALKIYQKNYHLNATDILDVETLTLLSMPR 84
           I  +K +LQ +GYL         + D + E AL  YQKN  L  T   D +TL+ + +PR
Sbjct: 50  IPEIKRHLQQYGYLPQ----NKESDDVSFEQALVRYQKNLGLPITGKPDSDTLSQILLPR 109

Query: 85  CGHPDIVNP---------DYVIAPGRPKWP-PTKYHLSYAFIND------FPPNFEEPVQ 144
           CG PD V P          YV  PGRP+W       L+YAF  +       P +     +
Sbjct: 110 CGFPDDVEPKTAPFHTGKKYVYFPGRPRWTRDVPLKLTYAFSQENLTPYLAPTDIRRVFR 169

Query: 145 CSLNTWTSVSNFTFSEATE-ATAD----FERGDHGDGVPFCG--PILAHSFFPTDGRLHF 204
            +   W SV   +F E  +   AD    F  GDHGDG PF G   +LAH+F P +GRLH 
Sbjct: 170 RAFGKWASVIPVSFIETEDYVIADIKIGFFNGDHGDGEPFDGVLGVLAHTFSPENGRLHL 229

Query: 205 NVDQNWAWG----AVAGSVDVQTVALHELGHILGLAHSQDECAIMYAYICPGTTKC-MSK 249
           +  + WA        + +VD+++VA+HE+GH+LGL HS  + A MY  + P + K  ++ 
Sbjct: 230 DKAETWAVDFDEEKSSVAVDLESVAVHEIGHVLGLGHSSVKDAAMYPTLKPRSKKVNLNM 289