MS016814 (gene) Bitter gourd (TR) v1

BLAST of MS016814 vs. NCBI nr
Match: XP_022144321.1 (uncharacterized protein LOC111014032 [Momordica charantia])

HSP 1 Score: 3484.5 bits (9034), Expect = 0.0e+00
Identity = 1812/1821 (99.51%), Postives = 1813/1821 (99.56%), Query Frame = 0

Query: 1    DVQGSENPIPLSPQWLLPKPGESKHGIGTGENHFSNPPAFRNRMDMMKASETYEDMNDMQ 60
            DVQGSENPIPLSPQWLLPKPGESKHGIGTGENHFSNPPAFRNRMDMMKASE YED NDMQ
Sbjct: 24   DVQGSENPIPLSPQWLLPKPGESKHGIGTGENHFSNPPAFRNRMDMMKASENYEDTNDMQ 83

Query: 61   KKKEVFRPSLADAEIGRRDRWHDEERENNSSMRKDRWRDGEKELGDSRKMDRWVEDSSTR 120
            KKKEVFRPSLADAEIGRRDRWHDEERENNSSMRKDRWRDGEKELGDSRKMDRWVEDSSTR
Sbjct: 84   KKKEVFRPSLADAEIGRRDRWHDEERENNSSMRKDRWRDGEKELGDSRKMDRWVEDSSTR 143

Query: 121  VFRESRRGPSERWSDLSNRDNVHYDQRRESKWNTRWGPDDKETEGFREKRVDSGRDGDLH 180
            VFRESRRGPSERWSDLSNRDNVHYDQRRESKWNTRWGPDDKETEGFREKRVDSGRDGDLH
Sbjct: 144  VFRESRRGPSERWSDLSNRDNVHYDQRRESKWNTRWGPDDKETEGFREKRVDSGRDGDLH 203

Query: 181  PDKNLSHVSNYGKNDRDGDHYRPWRSSASQGRGKGEPPHHQTQTPVKQVPAFSHRGRADN 240
            PDKNLSHVSNYGKNDRDGDHYRPWRSSASQGRGKGEPPHHQTQTPVKQVPAFSHRGRADN
Sbjct: 204  PDKNLSHVSNYGKNDRDGDHYRPWRSSASQGRGKGEPPHHQTQTPVKQVPAFSHRGRADN 263

Query: 241  TPPTFSLGRGIISSGVSSTNNIYSSSNSLGASSEKSGRDPYHYKYSRTKLLDVYRTTNLT 300
             PPTFSLGRGIISSGVSSTNNIYSSSNSLGASSEKSGRDPYHYKYSRTKLLDVYRTTNLT
Sbjct: 264  IPPTFSLGRGIISSGVSSTNNIYSSSNSLGASSEKSGRDPYHYKYSRTKLLDVYRTTNLT 323

Query: 301  LEPSLKDGFVPVPSLTLEEPLEPLALCAPSTEEMTFLKGIDKGEIISSGAPQVSKDGRNS 360
            LEPSLKDGFVPVPSLTLEEPLEPLALCAPSTEEMTFLKGIDKGEIISSGAPQVSKDGRNS
Sbjct: 324  LEPSLKDGFVPVPSLTLEEPLEPLALCAPSTEEMTFLKGIDKGEIISSGAPQVSKDGRNS 383

Query: 361  SEFMQTRRAKLGVSPSPAGSREDLPHGFDDYNDDKDDSTTKPGHINYSELSTERQMPPYH 420
            SEFMQTRRAKLGVSPSP GSREDLPHGFDDYNDDKDDSTTKPGHINYSELSTERQMPPYH
Sbjct: 384  SEFMQTRRAKLGVSPSP-GSREDLPHGFDDYNDDKDDSTTKPGHINYSELSTERQMPPYH 443

Query: 421  RPQSKIETIQEHMAHASGKFKSEAFREDDNAIRKADEVPVNRESSVKGGATVHPGSAWDA 480
            RPQSKIETIQEHMAHASGKFKSEAFREDDNAIRKADEVPVNRESSVKGGATVHPGSAWDA
Sbjct: 444  RPQSKIETIQEHMAHASGKFKSEAFREDDNAIRKADEVPVNRESSVKGGATVHPGSAWDA 503

Query: 481  SSLEQPLNTSVPDWRDNHNNIGSGTPDKGWLQPPKNLNDGWGSNTATPSYPKDNPKWQTG 540
            SSLEQPLNTSVPDWRDNHNNIGSGTPDKGWLQPPKNLNDGWGSNTATPSYPKDNPKWQTG
Sbjct: 504  SSLEQPLNTSVPDWRDNHNNIGSGTPDKGWLQPPKNLNDGWGSNTATPSYPKDNPKWQTG 563

Query: 541  EESIIRRQLSGILDKEQLARKTIQSAPEDMQLHYIDPSGAIQGPFSGADIIQWFEGGYFG 600
            EESIIRRQLSGILDKEQLARKTIQSAPEDMQLHYIDPSGAIQGPFSGADIIQWFEGGYFG
Sbjct: 564  EESIIRRQLSGILDKEQLARKTIQSAPEDMQLHYIDPSGAIQGPFSGADIIQWFEGGYFG 623

Query: 601  LDLPVRPVSAPNDLPFSALGDVMPHLRSKAKPPPGFSGPKQNEFGDALGNTSYGSLGKLH 660
            LDLPVRPVSAPNDLPFSALGDVMPHLRSKAKPPPGFSGPKQNEFGDALGNTSYGSLGKLH
Sbjct: 624  LDLPVRPVSAPNDLPFSALGDVMPHLRSKAKPPPGFSGPKQNEFGDALGNTSYGSLGKLH 683

Query: 661  TGLNEIDTLRNETRHKHGSTVEAENRFLESLMSGNLGSSPLEKSAFSEGYFGNNPNNLPS 720
            TGLNEIDTLRNET HKHGSTVEAENRFLESLMSGNLGSSPLEKSAFSEGYFGNNPNNLPS
Sbjct: 684  TGLNEIDTLRNETTHKHGSTVEAENRFLESLMSGNLGSSPLEKSAFSEGYFGNNPNNLPS 743

Query: 721  LGIDNGNNLFLLAKRMELERQRSLNNPYAFWPGIDATAKVSKPDVGLDDPIQQAKLLSSI 780
            LGIDNGNNLFLLAKRMELERQRSLNNPYAFWPGIDATAKVSKPDVGLDDPIQQAKLLSSI
Sbjct: 744  LGIDNGNNLFLLAKRMELERQRSLNNPYAFWPGIDATAKVSKPDVGLDDPIQQAKLLSSI 803

Query: 781  MDHSRQTSHSQSADMSAILQGLSDKAPPGINDVAAWSKFASQCASDPLQSKLDLHHDLNL 840
            MDHSRQTSHSQSADMSAILQGLSDKAPPGINDVAAWSKFASQCASDPLQSKLDLHHDLNL
Sbjct: 804  MDHSRQTSHSQSADMSAILQGLSDKAPPGINDVAAWSKFASQCASDPLQSKLDLHHDLNL 863

Query: 841  SSQAPFGFQQQRLQPQPSLTNLLAQATDNPTLTPDKFLSSSLSQDPQLISKLQQQHLLQL 900
            SSQAPFGFQQQRLQPQPSLTNLLAQATDNPTLTPDKFLSSSLSQDPQLISKLQQQHLLQL
Sbjct: 864  SSQAPFGFQQQRLQPQPSLTNLLAQATDNPTLTPDKFLSSSLSQDPQLISKLQQQHLLQL 923

Query: 901  HSQVPFSAQQMSLLDKLLLLKQQQKQEEQQQLLQQQQLLSQVLSEHQSRQHFGDPSFGQL 960
            HSQVPFSAQQMSLLDKLLLLKQQQKQEEQQQLLQQQQLLSQVLSEHQSRQHFGDPSFGQL
Sbjct: 924  HSQVPFSAQQMSLLDKLLLLKQQQKQEEQQQLLQQQQLLSQVLSEHQSRQHFGDPSFGQL 983

Query: 961  QGAPIPIGNASIDPSQVQLSREKFQIGSQKPLNVATTFGNMPLQVTQGASYNVNPEDPSL 1020
            QGAPIPIGNASIDPSQVQLSREKFQIGSQKPLNVATTFGNMPLQVTQGASYNVNPEDPSL
Sbjct: 984  QGAPIPIGNASIDPSQVQLSREKFQIGSQKPLNVATTFGNMPLQVTQGASYNVNPEDPSL 1043

Query: 1021 ALPHQMFGNVIQQKSWNAALPEQFNDTHPKDMLPGSKVGEGPTLPGMISKSSEDVNLVPH 1080
            ALPHQMFGNVIQQKSWNAALPEQFNDTHPKDMLPGSKVGEGPTLPGMISKSSEDVNLVPH
Sbjct: 1044 ALPHQMFGNVIQQKSWNAALPEQFNDTHPKDMLPGSKVGEGPTLPGMISKSSEDVNLVPH 1103

Query: 1081 SSDNNTNKVLEKASEDVPSLDATVTSLASDATIEPLPLKTAEVSVAIPPVEVRISEISIP 1140
            SSDNNTNKVLEKASEDVPSLDATVTSLASDATIEPLPLKTAEVSVAIPPVEVRISEISIP
Sbjct: 1104 SSDNNTNKVLEKASEDVPSLDATVTSLASDATIEPLPLKTAEVSVAIPPVEVRISEISIP 1163

Query: 1141 ESVPVLKVQEASMPVEKLGRDVVCKDETSLETELKNVEVQEPRKSSDKKTKKQKSSKLLS 1200
            ESVPVLKVQEASMPVEKLGRDVVCKDET LETELKNVEVQEPRKSSDKKTKKQKSSKLLS
Sbjct: 1164 ESVPVLKVQEASMPVEKLGRDVVCKDET-LETELKNVEVQEPRKSSDKKTKKQKSSKLLS 1223

Query: 1201 SDQAKDSKNSAIQQSKQSKSGKPELNDLKLKADSVVGKSSDTSSSPRKIRDADAKIAVVD 1260
            SDQAKDSKNSAIQQSKQSKSGKPELNDLKLKADSVVGKSSDTSSSPRKIRDADAKIAVVD
Sbjct: 1224 SDQAKDSKNSAIQQSKQSKSGKPELNDLKLKADSVVGKSSDTSSSPRKIRDADAKIAVVD 1283

Query: 1261 SQPFQRSASAVNSWNDAETVQVKDDSRLTGSDSVPNSQSQSGQRAWKTATSFRAKSLLEI 1320
            SQPFQ SASAVNSWNDAETVQVKDDSRLTGSDSVPNSQSQSGQRAWKTATSFRAKSLLEI
Sbjct: 1284 SQPFQSSASAVNSWNDAETVQVKDDSRLTGSDSVPNSQSQSGQRAWKTATSFRAKSLLEI 1343

Query: 1321 QEEEQKMAHTEPAVSEISISVNSTSLSTPWAGIVSNLEPKASREIHKDLVNSEPSEKHES 1380
            QEEEQKMAHTEPAVSEISISVNSTSLSTPWAGIVSNL+PKASREIHKD VNSEPSEKHES
Sbjct: 1344 QEEEQKMAHTEPAVSEISISVNSTSLSTPWAGIVSNLDPKASREIHKDFVNSEPSEKHES 1403

Query: 1381 LLNSRSRKSQLHDLLAEDDMEKSGGVGDVRVSDSVQIASSPQVMTTQAESTDDNFIEAKE 1440
            LLNSRSRKSQLHDLLAEDDMEKSGGVGDVRVSDSVQIASSPQVMTTQAESTDDNFIEAKE
Sbjct: 1404 LLNSRSRKSQLHDLLAEDDMEKSGGVGDVRVSDSVQIASSPQVMTTQAESTDDNFIEAKE 1463

Query: 1441 TKKSRKKSAKAKGVGTKASASAPSADVPVASSPVEKGKISRQTQQEKDATPAIPSGPSFG 1500
            TKKSRKKSAKAKGVGTKASASAPSADVPVASSPVEKGKISRQTQQEKDATPAIPSGPSFG
Sbjct: 1464 TKKSRKKSAKAKGVGTKASASAPSADVPVASSPVEKGKISRQTQQEKDATPAIPSGPSFG 1523

Query: 1501 DFVLWKGEAANVAPAPAWSSDSGKVHKPTSLRDIQKEQGRKLSSAQHSHQIPTPQKAQPT 1560
            DFVLWKGEAANVAPAPAWSSDSGKVHKPTSLRDIQKEQGRKLSSAQHSHQIPTPQKAQPT
Sbjct: 1524 DFVLWKGEAANVAPAPAWSSDSGKVHKPTSLRDIQKEQGRKLSSAQHSHQIPTPQKAQPT 1583

Query: 1561 QVGRSSSTSTPSWALSASSPSKAASPLQNIPSQSKYGGDDDLFWGPIESKQENQQVDVRQ 1620
            QVGRSSSTSTPSWALSASSPSKAASPLQNIPSQSKYGGDDDLFWGPIESKQENQQVDVRQ
Sbjct: 1584 QVGRSSSTSTPSWALSASSPSKAASPLQNIPSQSKYGGDDDLFWGPIESKQENQQVDVRQ 1643

Query: 1621 GSHSSWGNRNTPSKAAAASTGLLSRQKSSGGKADYPSSSPAQSTQKGKQDPITKHSEAMG 1680
            GSHSSWGNRNTPSKAAAASTGLLSRQKSSGGKADYPSSSPAQSTQKGKQDPITKHSEAMG
Sbjct: 1644 GSHSSWGNRNTPSKAAAASTGLLSRQKSSGGKADYPSSSPAQSTQKGKQDPITKHSEAMG 1703

Query: 1681 FRDWCESECVRLIGTKDTSFLEFCLKQSRSEAELLLIENLGSFDPDHEFIDQFLNYKELL 1740
            FRDWCESECVRLIGTKDTSFLEFCLKQSRSEAELLLIENLGSFDPDHEFIDQFLNYKELL
Sbjct: 1704 FRDWCESECVRLIGTKDTSFLEFCLKQSRSEAELLLIENLGSFDPDHEFIDQFLNYKELL 1763

Query: 1741 PADVLDIAFQRRNDRKVSEIATRDMNSGSAGGDLDPDVPVGSAKGGGKKKGKKGKKVSPS 1800
            PADVLDIAFQRRNDRKVSEIATRDMNSGSAGGDLDPDVPVGSAKGGGKKKGKKGKKVSPS
Sbjct: 1764 PADVLDIAFQRRNDRKVSEIATRDMNSGSAGGDLDPDVPVGSAKGGGKKKGKKGKKVSPS 1823

Query: 1801 VLGFNVVSNRIMMGEIQTVED 1822
            VLGFNVVSNRIMMGEIQTVED
Sbjct: 1824 VLGFNVVSNRIMMGEIQTVED 1842

BLAST of MS016814 vs. NCBI nr
Match: XP_038885397.1 (protein ESSENTIAL FOR POTEXVIRUS ACCUMULATION 1 isoform X2 [Benincasa hispida])

HSP 1 Score: 3037.7 bits (7874), Expect = 0.0e+00
Identity = 1594/1830 (87.10%), Postives = 1693/1830 (92.51%), Query Frame = 0

Query: 1    DVQGSENPIPLSPQWLLPKPGESKHGIGTGENHFSNPPAFRNRMDMMKASETYEDMNDMQ 60
            DVQGSENPIPLSPQWLLPKPGESKHGIGTGENHFS+ PA+ NRMD MK SE YED+ND+Q
Sbjct: 24   DVQGSENPIPLSPQWLLPKPGESKHGIGTGENHFSHQPAYGNRMDTMKGSENYEDINDIQ 83

Query: 61   KKKEVFRPSLADAEIGRRDRWHDEERENNSSMRKDRWRDGEKELGDSRKMDRWVEDSSTR 120
            KKKEVFRPSL D+EIGRRDRWHDEERENNSSMRKDRWRDGEKE+GDSRKMDRW EDSSTR
Sbjct: 84   KKKEVFRPSLTDSEIGRRDRWHDEERENNSSMRKDRWRDGEKEMGDSRKMDRWNEDSSTR 143

Query: 121  VFRESRRGPSERWSDLSNRDNVHYDQRRESKWNTRWGPDDKETEGFREKRVDSGRDGDLH 180
            VFRESRRGPSERWSD +NRDNVHYDQRRESKWNTRWGPDDKETEGFREKR+DSGRDGDLH
Sbjct: 144  VFRESRRGPSERWSDSNNRDNVHYDQRRESKWNTRWGPDDKETEGFREKRLDSGRDGDLH 203

Query: 181  PDKNLSHVSNYGKNDRDGDHYRPWRSSASQGRGKGEPPHHQTQTPVKQVPAFSHRGRADN 240
             DKNLSHVSNYGKNDRDGDHYRPWRS++SQ RGKG+PPHHQ+QTP+KQVPAFSHRGR DN
Sbjct: 204  LDKNLSHVSNYGKNDRDGDHYRPWRSNSSQVRGKGDPPHHQSQTPIKQVPAFSHRGRGDN 263

Query: 241  TPPTFSLGRGIISSGVSSTNNIYSSSNSLGASSEKSGRDPYHYKYSRTKLLDVYRTTNLT 300
            TPPTFSLGRGIISSGV+ TN+IYSSSN LGASSEKSGR+PYHYKYSRTKLLDV+RTTNLT
Sbjct: 264  TPPTFSLGRGIISSGVNPTNSIYSSSNYLGASSEKSGREPYHYKYSRTKLLDVFRTTNLT 323

Query: 301  LEPSLKDGFVPVPSLTLEEPLEPLALCAPSTEEMTFLKGIDKGEIISSGAPQVSKDGRNS 360
             + + KDGFVPVP+LTL+EPLEPLALCAP+TEEMTFLKGIDKGEI+SSGAPQVSKDGRNS
Sbjct: 324  SQQTPKDGFVPVPTLTLDEPLEPLALCAPTTEEMTFLKGIDKGEIVSSGAPQVSKDGRNS 383

Query: 361  SEFMQTRRAKLGVSPSPAGSREDLPHGF-DDYNDDKDDSTTKPGHINYSELSTERQMPPY 420
            SEFMQTRR KL VSPS  GSREDLPHGF DDYNDDKDD TTKPGH NYSE+STERQ+ PY
Sbjct: 384  SEFMQTRRTKLSVSPS-LGSREDLPHGFVDDYNDDKDDGTTKPGHTNYSEVSTERQV-PY 443

Query: 421  HRPQSKIETIQEHMAHASGKFKSEAFREDDNAIRKADEVPVNRESSVKGGATVHPGSAWD 480
            HRPQSKIE IQEHM H S  FKSEAFRED+NA+RK DEVPVNRESSVKGG  VHPGS WD
Sbjct: 444  HRPQSKIEAIQEHMTHTSSTFKSEAFREDNNAMRKTDEVPVNRESSVKGGTNVHPGSTWD 503

Query: 481  ASSLEQPLNTSVPDWRDNHNNIGSGTPDKGWLQPPKNLNDGWGSNTATPSYPKDNPKWQT 540
            ASSLEQPLNTS+PD R+N NNI SGTPDKGWLQ  KNLNDGWGSNTATPSYPKDNPKWQT
Sbjct: 504  ASSLEQPLNTSLPDRRENPNNISSGTPDKGWLQSSKNLNDGWGSNTATPSYPKDNPKWQT 563

Query: 541  GEESIIRRQLSGILDKEQLARKTIQSAPEDMQLHYIDPSGAIQGPFSGADIIQWFEGGYF 600
            GEESIIRRQLSGILDKEQLARKT+QSAPED+QLHYIDPSGAIQGPFSGADIIQWFEGGYF
Sbjct: 564  GEESIIRRQLSGILDKEQLARKTVQSAPEDLQLHYIDPSGAIQGPFSGADIIQWFEGGYF 623

Query: 601  GLDLPVRPVSAPNDLPFSALGDVMPHLRSKAKPPPGFSGPKQNEFGDALGNTSYGSLGKL 660
            GLDLPVRPV+APNDLPF+ALGDVMPHLRSKAKPPPGFSGPKQNEF D+LGN SYGSLGKL
Sbjct: 624  GLDLPVRPVNAPNDLPFAALGDVMPHLRSKAKPPPGFSGPKQNEFADSLGNASYGSLGKL 683

Query: 661  HTGLNEIDTLRNETRHKHGSTVEAENRFLESLMSGNLGSSPLEKSAFSEGYFGNNPNNLP 720
            HTGLNEIDT+RNETRHKHGSTVEAENRFLESLMSGN+GSSPLEK+AFSEGYFGNN ++L 
Sbjct: 684  HTGLNEIDTMRNETRHKHGSTVEAENRFLESLMSGNIGSSPLEKNAFSEGYFGNNSSSLS 743

Query: 721  SLGIDNGNNLFLLAKRMELERQRSLNNPYAFWPGIDATAKVSKPDVGLDDPIQQAKLLSS 780
            SLGIDNGNNLFLLAKRMELERQRSL+NPYAFWPGIDAT+KVSKPD+GLDDPIQQAKLLSS
Sbjct: 744  SLGIDNGNNLFLLAKRMELERQRSLSNPYAFWPGIDATSKVSKPDIGLDDPIQQAKLLSS 803

Query: 781  IMDHSRQTSHSQSADMSAILQGLSDKAPPGINDVAAWSKFASQCASDPLQSKLDLHHDLN 840
            I+DHSRQTSHSQ ADMSAILQGLSDKAPPGINDVA WSKFA QCA DPLQSKLDLHHDLN
Sbjct: 804  IIDHSRQTSHSQGADMSAILQGLSDKAPPGINDVAGWSKFA-QCAPDPLQSKLDLHHDLN 863

Query: 841  LSSQAPFGFQQQRLQPQPSLTNLLAQATDNPTLTPDKFLSSSLSQDPQLISKLQQQHLLQ 900
            LSSQAPFGFQQQRLQPQPSLTNLLAQATDNPTLTPDKFL SSLSQDPQLISKLQQQHLLQ
Sbjct: 864  LSSQAPFGFQQQRLQPQPSLTNLLAQATDNPTLTPDKFLPSSLSQDPQLISKLQQQHLLQ 923

Query: 901  LHSQVPFSAQQMSLLDKLLLLKQQQKQEEQQQLLQQQQLLSQVLSEHQSRQHFGDPSFGQ 960
            LHSQVPFSAQQMSLLDKLLLLKQQQKQEEQQQLLQQQQLLSQVLSEHQSRQHF DPSFGQ
Sbjct: 924  LHSQVPFSAQQMSLLDKLLLLKQQQKQEEQQQLLQQQQLLSQVLSEHQSRQHFIDPSFGQ 983

Query: 961  LQGAPIPIGNASIDPSQVQLSREKFQIGSQKPLNVAT----TFGNMPLQVTQGASYNVNP 1020
            LQGAP+PIGNAS DP+QVQ SREKFQIGSQKPLNV T     FGNM LQVTQGASYNVN 
Sbjct: 984  LQGAPMPIGNASTDPAQVQQSREKFQIGSQKPLNVVTDRAAPFGNMALQVTQGASYNVNL 1043

Query: 1021 EDPSLALPHQMFGNVIQQKSWNAALPEQFNDTHPKDMLPGSKVGEGPTLPGMISKSSEDV 1080
            EDP LALPHQMFGNV+QQKSW  AL EQ NDT P DMLPGSKV EG   PG+ ++S+EDV
Sbjct: 1044 EDPPLALPHQMFGNVVQQKSWTPALSEQLNDTCPNDMLPGSKVAEGSLFPGLTAQSNEDV 1103

Query: 1081 NLVPHSSDNNTNKVLEKASEDVPSLDATVTSLASDATIEPLPLKTAEVSVAIPPVEVRIS 1140
            NLVP SSD++T+K LE+ +EDVP LDATVTSLASDA +EPLPLKTAE+SVAI P EV   
Sbjct: 1104 NLVPKSSDSHTSKALEQIAEDVPRLDATVTSLASDALVEPLPLKTAEISVAIQPAEVHDF 1163

Query: 1141 EISIPESVPVLKVQEASMPVEKLGRDVVCKDETSLETELKNVEVQEPRKSSDKKTKKQKS 1200
            EISIP+SVP LKVQEASMPVEKL RD VCKDETSLETELKNVEVQEPRKSSDKKTKKQKS
Sbjct: 1164 EISIPDSVPALKVQEASMPVEKLERD-VCKDETSLETELKNVEVQEPRKSSDKKTKKQKS 1223

Query: 1201 SKLLSSDQAKDSKNSAIQQSKQSKSGKPELNDLKLKADSVVGKSSDTSSSPRKIRDADAK 1260
            SKLLSSDQAKDSK+SAIQQ KQSKSGK E NDLKLKAD++VGKSSDTSSSPRKIRD D K
Sbjct: 1224 SKLLSSDQAKDSKSSAIQQLKQSKSGKSE-NDLKLKADNIVGKSSDTSSSPRKIRDGDGK 1283

Query: 1261 IAVVDSQPFQRSASAVNSWNDAETVQVKDDSRLTGSDSVPNSQSQSGQRAWKTATSFRAK 1320
             +VVDSQP Q SASA+N+WND ET+Q+KDD+RL GSDSV NSQ+QSGQRAWK A+SF+ K
Sbjct: 1284 TSVVDSQPVQSSASAINTWNDGETIQMKDDARLVGSDSVLNSQTQSGQRAWKVASSFKPK 1343

Query: 1321 SLLEIQEEEQKMAHTEPAVSEISISVNSTSLSTPWAGIVSNLEPKASREIHKDLVNSEPS 1380
            SLLEIQEEEQK AHTE AVS+IS S+NS SLSTPWAGIVS+ +PK SREIHKD   SE S
Sbjct: 1344 SLLEIQEEEQKRAHTETAVSDISTSINSMSLSTPWAGIVSSSDPKTSREIHKDSGISESS 1403

Query: 1381 EKHESLLNSRSRKSQLHDLLAEDDMEKSGGVGDVRVSDSVQIASSPQVMTTQAESTDDNF 1440
            EKHE+LL SRSRKSQLHDLLAEDDMEKS   GDVRVSDSVQIASSP+VM TQAE  DDNF
Sbjct: 1404 EKHENLLTSRSRKSQLHDLLAEDDMEKS-TAGDVRVSDSVQIASSPRVMATQAEPLDDNF 1463

Query: 1441 IEAKETKKSRKKSAKAKGVGTKASASAPSADVPVASSPVEKGKISRQTQQEKDATPAIPS 1500
            IEAK+TKKSRKKSAKAKGVGTKA+ + PSADV VASSP+EKGKISRQTQQEK+A PAIPS
Sbjct: 1464 IEAKDTKKSRKKSAKAKGVGTKATPAVPSADV-VASSPIEKGKISRQTQQEKEAMPAIPS 1523

Query: 1501 GPSFGDFVLWKGEAANVAPAPAWSSDSGKVHKPTSLRDIQKEQGRKLSSAQHSHQIPTPQ 1560
            GPSFGDFVLWKGE ANVAP PAWSSDSGKV KPTSLRDIQKEQGRK+S+AQHSHQ PTPQ
Sbjct: 1524 GPSFGDFVLWKGETANVAPPPAWSSDSGKVPKPTSLRDIQKEQGRKISAAQHSHQNPTPQ 1583

Query: 1561 KAQPTQVGRSSSTSTPSWALSASSPSKAA-SPLQNIPSQSKYGGDDDLFWGPIESKQENQ 1620
            KAQPTQ GRSSSTSTPSWALSASSPSKAA SPLQNIP+QSK+GGDDDLFWGPIE+KQENQ
Sbjct: 1584 KAQPTQAGRSSSTSTPSWALSASSPSKAASSPLQNIPTQSKHGGDDDLFWGPIEAKQENQ 1643

Query: 1621 QVDVRQGSHSSWGNRNTPSKAAAASTGLLSRQKSSGGKADYPSSSPAQSTQKGKQDPITK 1680
            QVDVR GSH SWGNR+  +K A ASTGLLSRQKSSGGKADY SSSPAQS+QKGKQDP+TK
Sbjct: 1644 QVDVRLGSHGSWGNRSASAK-AVASTGLLSRQKSSGGKADYLSSSPAQSSQKGKQDPVTK 1703

Query: 1681 HSEAMGFRDWCESECVRLIGTKDTSFLEFCLKQSRSEAELLLIENLGSFDPDHEFIDQFL 1740
            HSEAMGFRDWCESECVRLIGTKDTSFLEFCLKQSRSEAEL LIENLGS+DPDH+FIDQFL
Sbjct: 1704 HSEAMGFRDWCESECVRLIGTKDTSFLEFCLKQSRSEAELFLIENLGSYDPDHDFIDQFL 1763

Query: 1741 NYKELLPADVLDIAFQRRNDRKVSEIATRDMNSGSAGGDLDPDVPV---GSAKGGGKKKG 1800
            NYKELLPADVL+IAFQ RNDRKVS IA+R++NSG+AGGD+DPDVP+   GSAK GGKKKG
Sbjct: 1764 NYKELLPADVLEIAFQSRNDRKVSAIASREVNSGNAGGDVDPDVPLGRDGSAKSGGKKKG 1823

Query: 1801 KKGKKVSPSVLGFNVVSNRIMMGEIQTVED 1822
            KKGKKVSPSVLGFNVVSNRIMMGEIQTVED
Sbjct: 1824 KKGKKVSPSVLGFNVVSNRIMMGEIQTVED 1845

BLAST of MS016814 vs. NCBI nr
Match: XP_038885396.1 (protein ESSENTIAL FOR POTEXVIRUS ACCUMULATION 1 isoform X1 [Benincasa hispida])

HSP 1 Score: 3032.3 bits (7860), Expect = 0.0e+00
Identity = 1594/1833 (86.96%), Postives = 1693/1833 (92.36%), Query Frame = 0

Query: 1    DVQGSENPIPLSPQWLLPKPGESKHGIGTGENHFSNPPAFRNRMDMMKASETYEDMNDMQ 60
            DVQGSENPIPLSPQWLLPKPGESKHGIGTGENHFS+ PA+ NRMD MK SE YED+ND+Q
Sbjct: 24   DVQGSENPIPLSPQWLLPKPGESKHGIGTGENHFSHQPAYGNRMDTMKGSENYEDINDIQ 83

Query: 61   KKKEVFRPSLADAEIGRRDRWHDEERENNSSMRKDRWRDGEKELGDSRKMDRWVEDSSTR 120
            KKKEVFRPSL D+EIGRRDRWHDEERENNSSMRKDRWRDGEKE+GDSRKMDRW EDSSTR
Sbjct: 84   KKKEVFRPSLTDSEIGRRDRWHDEERENNSSMRKDRWRDGEKEMGDSRKMDRWNEDSSTR 143

Query: 121  VFRESRRGPSERWSDLSNRDNVHYDQRRESKWNTRWGPDDKETEGFREKRVDSGRDGDLH 180
            VFRESRRGPSERWSD +NRDNVHYDQRRESKWNTRWGPDDKETEGFREKR+DSGRDGDLH
Sbjct: 144  VFRESRRGPSERWSDSNNRDNVHYDQRRESKWNTRWGPDDKETEGFREKRLDSGRDGDLH 203

Query: 181  PDKNLSHVSNYGKNDRDGDHYRPWRSSASQGRGKGEPPHHQTQTPVKQVPAFSHRGRADN 240
             DKNLSHVSNYGKNDRDGDHYRPWRS++SQ RGKG+PPHHQ+QTP+KQVPAFSHRGR DN
Sbjct: 204  LDKNLSHVSNYGKNDRDGDHYRPWRSNSSQVRGKGDPPHHQSQTPIKQVPAFSHRGRGDN 263

Query: 241  TPPTFSLGRGIISSGVSSTNNIYSSSNSLGASSEKSGRDPYHYKYSRTKLLDVYRTTNLT 300
            TPPTFSLGRGIISSGV+ TN+IYSSSN LGASSEKSGR+PYHYKYSRTKLLDV+RTTNLT
Sbjct: 264  TPPTFSLGRGIISSGVNPTNSIYSSSNYLGASSEKSGREPYHYKYSRTKLLDVFRTTNLT 323

Query: 301  LEPSLKDGFVPVPSLTLEEPLEPLALCAPSTEEMTFLKGIDKGEIISSGAPQVSKDGRNS 360
             + + KDGFVPVP+LTL+EPLEPLALCAP+TEEMTFLKGIDKGEI+SSGAPQVSKDGRNS
Sbjct: 324  SQQTPKDGFVPVPTLTLDEPLEPLALCAPTTEEMTFLKGIDKGEIVSSGAPQVSKDGRNS 383

Query: 361  SEFMQTRRAKLGVSPSPAGSREDLPHGF-DDYNDDKDDSTTKPGHINYSELSTERQMPPY 420
            SEFMQTRR KL VSPS  GSREDLPHGF DDYNDDKDD TTKPGH NYSE+STERQ+ PY
Sbjct: 384  SEFMQTRRTKLSVSPS-LGSREDLPHGFVDDYNDDKDDGTTKPGHTNYSEVSTERQV-PY 443

Query: 421  HRPQSKIETIQEHMAHASGKFKSEAFREDDNAIRKADEVPVNRESSVKGGATVHPGSAWD 480
            HRPQSKIE IQEHM H S  FKSEAFRED+NA+RK DEVPVNRESSVKGG  VHPGS WD
Sbjct: 444  HRPQSKIEAIQEHMTHTSSTFKSEAFREDNNAMRKTDEVPVNRESSVKGGTNVHPGSTWD 503

Query: 481  ASSLEQPLNTSVPDWRDNHNNIGSGTPDKGWLQPPKNLNDGWGSNTATPSYPKDNPKWQT 540
            ASSLEQPLNTS+PD R+N NNI SGTPDKGWLQ  KNLNDGWGSNTATPSYPKDNPKWQT
Sbjct: 504  ASSLEQPLNTSLPDRRENPNNISSGTPDKGWLQSSKNLNDGWGSNTATPSYPKDNPKWQT 563

Query: 541  GEESIIRRQLSGILDKEQLARKTIQSAPEDMQLHYIDPSGAIQGPFSGADIIQWFEGGYF 600
            GEESIIRRQLSGILDKEQLARKT+QSAPED+QLHYIDPSGAIQGPFSGADIIQWFEGGYF
Sbjct: 564  GEESIIRRQLSGILDKEQLARKTVQSAPEDLQLHYIDPSGAIQGPFSGADIIQWFEGGYF 623

Query: 601  GLDLPVRPVSAPNDLPFSALGDVMPHLRSKAKPPPGFSGPKQNEFGDALGNTSYGSLGKL 660
            GLDLPVRPV+APNDLPF+ALGDVMPHLRSKAKPPPGFSGPKQNEF D+LGN SYGSLGKL
Sbjct: 624  GLDLPVRPVNAPNDLPFAALGDVMPHLRSKAKPPPGFSGPKQNEFADSLGNASYGSLGKL 683

Query: 661  HTGLNEIDTLRNETRHKHGSTVEAENRFLESLMSGNLGSSPLEKSAFSE---GYFGNNPN 720
            HTGLNEIDT+RNETRHKHGSTVEAENRFLESLMSGN+GSSPLEK+AFSE   GYFGNN +
Sbjct: 684  HTGLNEIDTMRNETRHKHGSTVEAENRFLESLMSGNIGSSPLEKNAFSEGVPGYFGNNSS 743

Query: 721  NLPSLGIDNGNNLFLLAKRMELERQRSLNNPYAFWPGIDATAKVSKPDVGLDDPIQQAKL 780
            +L SLGIDNGNNLFLLAKRMELERQRSL+NPYAFWPGIDAT+KVSKPD+GLDDPIQQAKL
Sbjct: 744  SLSSLGIDNGNNLFLLAKRMELERQRSLSNPYAFWPGIDATSKVSKPDIGLDDPIQQAKL 803

Query: 781  LSSIMDHSRQTSHSQSADMSAILQGLSDKAPPGINDVAAWSKFASQCASDPLQSKLDLHH 840
            LSSI+DHSRQTSHSQ ADMSAILQGLSDKAPPGINDVA WSKFA QCA DPLQSKLDLHH
Sbjct: 804  LSSIIDHSRQTSHSQGADMSAILQGLSDKAPPGINDVAGWSKFA-QCAPDPLQSKLDLHH 863

Query: 841  DLNLSSQAPFGFQQQRLQPQPSLTNLLAQATDNPTLTPDKFLSSSLSQDPQLISKLQQQH 900
            DLNLSSQAPFGFQQQRLQPQPSLTNLLAQATDNPTLTPDKFL SSLSQDPQLISKLQQQH
Sbjct: 864  DLNLSSQAPFGFQQQRLQPQPSLTNLLAQATDNPTLTPDKFLPSSLSQDPQLISKLQQQH 923

Query: 901  LLQLHSQVPFSAQQMSLLDKLLLLKQQQKQEEQQQLLQQQQLLSQVLSEHQSRQHFGDPS 960
            LLQLHSQVPFSAQQMSLLDKLLLLKQQQKQEEQQQLLQQQQLLSQVLSEHQSRQHF DPS
Sbjct: 924  LLQLHSQVPFSAQQMSLLDKLLLLKQQQKQEEQQQLLQQQQLLSQVLSEHQSRQHFIDPS 983

Query: 961  FGQLQGAPIPIGNASIDPSQVQLSREKFQIGSQKPLNVAT----TFGNMPLQVTQGASYN 1020
            FGQLQGAP+PIGNAS DP+QVQ SREKFQIGSQKPLNV T     FGNM LQVTQGASYN
Sbjct: 984  FGQLQGAPMPIGNASTDPAQVQQSREKFQIGSQKPLNVVTDRAAPFGNMALQVTQGASYN 1043

Query: 1021 VNPEDPSLALPHQMFGNVIQQKSWNAALPEQFNDTHPKDMLPGSKVGEGPTLPGMISKSS 1080
            VN EDP LALPHQMFGNV+QQKSW  AL EQ NDT P DMLPGSKV EG   PG+ ++S+
Sbjct: 1044 VNLEDPPLALPHQMFGNVVQQKSWTPALSEQLNDTCPNDMLPGSKVAEGSLFPGLTAQSN 1103

Query: 1081 EDVNLVPHSSDNNTNKVLEKASEDVPSLDATVTSLASDATIEPLPLKTAEVSVAIPPVEV 1140
            EDVNLVP SSD++T+K LE+ +EDVP LDATVTSLASDA +EPLPLKTAE+SVAI P EV
Sbjct: 1104 EDVNLVPKSSDSHTSKALEQIAEDVPRLDATVTSLASDALVEPLPLKTAEISVAIQPAEV 1163

Query: 1141 RISEISIPESVPVLKVQEASMPVEKLGRDVVCKDETSLETELKNVEVQEPRKSSDKKTKK 1200
               EISIP+SVP LKVQEASMPVEKL RD VCKDETSLETELKNVEVQEPRKSSDKKTKK
Sbjct: 1164 HDFEISIPDSVPALKVQEASMPVEKLERD-VCKDETSLETELKNVEVQEPRKSSDKKTKK 1223

Query: 1201 QKSSKLLSSDQAKDSKNSAIQQSKQSKSGKPELNDLKLKADSVVGKSSDTSSSPRKIRDA 1260
            QKSSKLLSSDQAKDSK+SAIQQ KQSKSGK E NDLKLKAD++VGKSSDTSSSPRKIRD 
Sbjct: 1224 QKSSKLLSSDQAKDSKSSAIQQLKQSKSGKSE-NDLKLKADNIVGKSSDTSSSPRKIRDG 1283

Query: 1261 DAKIAVVDSQPFQRSASAVNSWNDAETVQVKDDSRLTGSDSVPNSQSQSGQRAWKTATSF 1320
            D K +VVDSQP Q SASA+N+WND ET+Q+KDD+RL GSDSV NSQ+QSGQRAWK A+SF
Sbjct: 1284 DGKTSVVDSQPVQSSASAINTWNDGETIQMKDDARLVGSDSVLNSQTQSGQRAWKVASSF 1343

Query: 1321 RAKSLLEIQEEEQKMAHTEPAVSEISISVNSTSLSTPWAGIVSNLEPKASREIHKDLVNS 1380
            + KSLLEIQEEEQK AHTE AVS+IS S+NS SLSTPWAGIVS+ +PK SREIHKD   S
Sbjct: 1344 KPKSLLEIQEEEQKRAHTETAVSDISTSINSMSLSTPWAGIVSSSDPKTSREIHKDSGIS 1403

Query: 1381 EPSEKHESLLNSRSRKSQLHDLLAEDDMEKSGGVGDVRVSDSVQIASSPQVMTTQAESTD 1440
            E SEKHE+LL SRSRKSQLHDLLAEDDMEKS   GDVRVSDSVQIASSP+VM TQAE  D
Sbjct: 1404 ESSEKHENLLTSRSRKSQLHDLLAEDDMEKS-TAGDVRVSDSVQIASSPRVMATQAEPLD 1463

Query: 1441 DNFIEAKETKKSRKKSAKAKGVGTKASASAPSADVPVASSPVEKGKISRQTQQEKDATPA 1500
            DNFIEAK+TKKSRKKSAKAKGVGTKA+ + PSADV VASSP+EKGKISRQTQQEK+A PA
Sbjct: 1464 DNFIEAKDTKKSRKKSAKAKGVGTKATPAVPSADV-VASSPIEKGKISRQTQQEKEAMPA 1523

Query: 1501 IPSGPSFGDFVLWKGEAANVAPAPAWSSDSGKVHKPTSLRDIQKEQGRKLSSAQHSHQIP 1560
            IPSGPSFGDFVLWKGE ANVAP PAWSSDSGKV KPTSLRDIQKEQGRK+S+AQHSHQ P
Sbjct: 1524 IPSGPSFGDFVLWKGETANVAPPPAWSSDSGKVPKPTSLRDIQKEQGRKISAAQHSHQNP 1583

Query: 1561 TPQKAQPTQVGRSSSTSTPSWALSASSPSKAA-SPLQNIPSQSKYGGDDDLFWGPIESKQ 1620
            TPQKAQPTQ GRSSSTSTPSWALSASSPSKAA SPLQNIP+QSK+GGDDDLFWGPIE+KQ
Sbjct: 1584 TPQKAQPTQAGRSSSTSTPSWALSASSPSKAASSPLQNIPTQSKHGGDDDLFWGPIEAKQ 1643

Query: 1621 ENQQVDVRQGSHSSWGNRNTPSKAAAASTGLLSRQKSSGGKADYPSSSPAQSTQKGKQDP 1680
            ENQQVDVR GSH SWGNR+  +K A ASTGLLSRQKSSGGKADY SSSPAQS+QKGKQDP
Sbjct: 1644 ENQQVDVRLGSHGSWGNRSASAK-AVASTGLLSRQKSSGGKADYLSSSPAQSSQKGKQDP 1703

Query: 1681 ITKHSEAMGFRDWCESECVRLIGTKDTSFLEFCLKQSRSEAELLLIENLGSFDPDHEFID 1740
            +TKHSEAMGFRDWCESECVRLIGTKDTSFLEFCLKQSRSEAEL LIENLGS+DPDH+FID
Sbjct: 1704 VTKHSEAMGFRDWCESECVRLIGTKDTSFLEFCLKQSRSEAELFLIENLGSYDPDHDFID 1763

Query: 1741 QFLNYKELLPADVLDIAFQRRNDRKVSEIATRDMNSGSAGGDLDPDVPV---GSAKGGGK 1800
            QFLNYKELLPADVL+IAFQ RNDRKVS IA+R++NSG+AGGD+DPDVP+   GSAK GGK
Sbjct: 1764 QFLNYKELLPADVLEIAFQSRNDRKVSAIASREVNSGNAGGDVDPDVPLGRDGSAKSGGK 1823

Query: 1801 KKGKKGKKVSPSVLGFNVVSNRIMMGEIQTVED 1822
            KKGKKGKKVSPSVLGFNVVSNRIMMGEIQTVED
Sbjct: 1824 KKGKKGKKVSPSVLGFNVVSNRIMMGEIQTVED 1848

BLAST of MS016814 vs. NCBI nr
Match: KAA0065243.1 (uncharacterized protein E6C27_scaffold82G005840 [Cucumis melo var. makuwa])

HSP 1 Score: 2940.2 bits (7621), Expect = 0.0e+00
Identity = 1558/1843 (84.54%), Postives = 1669/1843 (90.56%), Query Frame = 0

Query: 1    DVQGSENPIPLSPQWLLPKPGESKHGIGTGENHFSNPPAFRNRMDMMKASETYEDMNDMQ 60
            DVQGSENPIPLSPQWLLPKPGESKHGIGTGENHFS+ PA+ NRMDMMK SE YEDMND Q
Sbjct: 24   DVQGSENPIPLSPQWLLPKPGESKHGIGTGENHFSHQPAYGNRMDMMKGSENYEDMNDTQ 83

Query: 61   KKKEVFRPSLADAEIGRRDRWHDEERENNSSMRKDRWRDGEKELGDSRKMDRWVEDSSTR 120
            KKKEVFRPS+ D+EIGRRDRWHDEERENNSSMRKDRWRDGEKE+GD RKMDRW EDSSTR
Sbjct: 84   KKKEVFRPSVTDSEIGRRDRWHDEERENNSSMRKDRWRDGEKEMGDGRKMDRWNEDSSTR 143

Query: 121  VFRESRRGPSERWSDLSNRDNVHYDQRRESKWNTRWGPDDKETEGFREKRVDSGRDGDLH 180
            VFRESRRGPSERWSD +NRDNVHYDQRRESKWNTRWGPDDKETEGFREKRVDSGRDGDLH
Sbjct: 144  VFRESRRGPSERWSDSNNRDNVHYDQRRESKWNTRWGPDDKETEGFREKRVDSGRDGDLH 203

Query: 181  PDKNLSHVSNYGKNDRDGDHYRPWRSSASQGRGKGEPPHHQTQTPVKQVPAFSHRGRADN 240
             DKN SHVSNYGKNDRDGDHYRPWRSS++QGRGKGEPPHHQTQTP KQVPAFSHRGRADN
Sbjct: 204  LDKNFSHVSNYGKNDRDGDHYRPWRSSSAQGRGKGEPPHHQTQTPSKQVPAFSHRGRADN 263

Query: 241  TPPTFSLGRGIISSGVSSTNNIYSSSNSLGASSEKSGRDPYHYKYSRTKLLDVYRTTNLT 300
            TPPTFSLGRGIISSGV+ TN++YSS N LGASSEKSGR+P +YKYSRTKLLDV+RTTNLT
Sbjct: 264  TPPTFSLGRGIISSGVNPTNSVYSSPNYLGASSEKSGREPCYYKYSRTKLLDVFRTTNLT 323

Query: 301  LEPSLKDGFVPVPSLTLEEPLEPLALCAPSTEEMTFLKGIDKGEIISSGAPQVSKDGRNS 360
             + +LKDGFVPVP+LTL+EPLEPLALCAP+TEEMTFLKGIDKGEI+SSGAPQVSKDGRNS
Sbjct: 324  SQQTLKDGFVPVPTLTLDEPLEPLALCAPTTEEMTFLKGIDKGEIVSSGAPQVSKDGRNS 383

Query: 361  SEFMQTRRAKLGVSPSPAGSREDLPHGFDDYNDDKDDSTTKPGHINYSELSTERQMPPYH 420
            SEFMQ RR KLGVSPS  GSREDLPHGFDDYNDDKDDSTTK GH NYSE+STERQ+ PYH
Sbjct: 384  SEFMQARRTKLGVSPS-LGSREDLPHGFDDYNDDKDDSTTKLGHTNYSEVSTERQV-PYH 443

Query: 421  RPQSKIETIQEHMAHASGKFKSEAFREDDNAIRKADEVPVNRESSVKGGATVHPGSAWDA 480
            RPQSK E IQE M H SG FKSEAFREDDNA+RK DEVP NRESSVKG   +H  S WDA
Sbjct: 444  RPQSKNEAIQEQMGHTSGNFKSEAFREDDNALRKTDEVPGNRESSVKGATNIHSSSTWDA 503

Query: 481  SSLEQPLNTSVPDWRDNHNN-IGSGTPDKGWLQPPKNLNDGWGSNTATPSYPKDNPKWQT 540
            SSLEQ LNTS+PDWRDN NN I SGTPDKGW+Q  KNL+DGWGSNT TPSY KDN KWQ+
Sbjct: 504  SSLEQSLNTSLPDWRDNPNNIISSGTPDKGWVQSSKNLSDGWGSNTTTPSYAKDNSKWQS 563

Query: 541  GEESIIRRQLSGILDKEQLARKTIQSAPEDMQLHYIDPSGAIQGPFSGADIIQWFEGGYF 600
             EESIIRRQLSGILDKEQL+RKT+Q APEDMQLHYIDPSGAIQGPF GADIIQWFEGGYF
Sbjct: 564  TEESIIRRQLSGILDKEQLSRKTVQPAPEDMQLHYIDPSGAIQGPFGGADIIQWFEGGYF 623

Query: 601  GLDLPVRPVSAPNDLPFSALGDVMPHLRSKAKPPPGFSGPKQNEFGDALGNTSYGSLGKL 660
            GLDLPVRP +AP+DLPFSALGDVMPHLRSKAKPPPGFSGPKQNEF D+LGN SYGSLGKL
Sbjct: 624  GLDLPVRPTNAPSDLPFSALGDVMPHLRSKAKPPPGFSGPKQNEFADSLGNASYGSLGKL 683

Query: 661  HTGLNEIDTLRNETRHKHGSTVEAENRFLESLMSGNLGSSPLEKSAFSE---GYFGNNPN 720
            HTGLNEIDT+RNETRHKHGSTVEAENRFLESLMSGN+GSSPLEKSAFSE   GYFG NPN
Sbjct: 684  HTGLNEIDTMRNETRHKHGSTVEAENRFLESLMSGNIGSSPLEKSAFSEGVPGYFGTNPN 743

Query: 721  NLPSLGIDNGNNLFLLAKRMELERQRSLNNPYAFWPGIDATAKVSKPDVGLDDPIQQAKL 780
            +L SLG+DNGNNLFLLAKRMELERQRS++NPYAFWPGIDAT+KVSKPD+GLDDPIQQAKL
Sbjct: 744  SLSSLGMDNGNNLFLLAKRMELERQRSMSNPYAFWPGIDATSKVSKPDIGLDDPIQQAKL 803

Query: 781  LSSIMDHSRQTSHSQSADMSAILQGLSDKAPPGINDVAAWSKFASQCASDPLQSKLDLHH 840
            LSSI+DHSRQTSHSQS DMSAILQGLSDKAPPGIN+VA WSKFA   A DPLQSKLDLHH
Sbjct: 804  LSSIIDHSRQTSHSQSPDMSAILQGLSDKAPPGINEVAGWSKFAH--APDPLQSKLDLHH 863

Query: 841  DLNLSSQAPFGFQQQRLQPQPSLTNLLAQATDNPTLTPDKFLSSSLSQDPQLISKLQQQH 900
            +LNLSSQAPFGFQQQRLQPQPSLTNLLAQATDNPTLTPDKFL SSLSQDPQLISKLQQQH
Sbjct: 864  ELNLSSQAPFGFQQQRLQPQPSLTNLLAQATDNPTLTPDKFLPSSLSQDPQLISKLQQQH 923

Query: 901  LLQLHSQVPFSAQQMSLLDKLLLLKQQQKQEEQQQLLQQQQLLSQVLSEHQSRQHFGDPS 960
            LLQLHSQVPFSAQQMSLLDKLLLLKQQQKQEEQQQLLQQQQLLSQVLSEHQSRQH  DPS
Sbjct: 924  LLQLHSQVPFSAQQMSLLDKLLLLKQQQKQEEQQQLLQQQQLLSQVLSEHQSRQHLIDPS 983

Query: 961  FGQLQGAPIPIGNASIDPSQVQLSREKFQIGSQKPLNVAT----TFGNMPLQVTQGASYN 1020
            FGQLQGAPIPIGNAS DPSQVQ  REKFQIGSQKPLNV T     FGNM LQVTQGASYN
Sbjct: 984  FGQLQGAPIPIGNASTDPSQVQQPREKFQIGSQKPLNVVTDRAIPFGNMALQVTQGASYN 1043

Query: 1021 VNPEDPSLALPHQMFGNVIQQKSWNAALPEQFNDTHPKDMLPGSKVGEGPTLPGMISKSS 1080
            VNPEDPSLALPHQMFGNV QQK W   LPEQ  DT PKDMLPGS VGE    PG+ SK  
Sbjct: 1044 VNPEDPSLALPHQMFGNV-QQKGWTPGLPEQLTDTRPKDMLPGSIVGEASLFPGLTSKQI 1103

Query: 1081 EDVNLVPHSSDNNTNKVLEKASEDVPSLDATVTSLASDATIEPLPLKTAEVSVAIPPVEV 1140
            EDV+ V  SSD++T + LE+  E VP LD T TSLASDA +EPLPLKTA++SVA+ P EV
Sbjct: 1104 EDVSHVQKSSDSHTVQALEQIGEAVPRLDETATSLASDAMVEPLPLKTADISVALQPAEV 1163

Query: 1141 RISEISIP--------ESVPVLKVQEASMPVEKLGRDVVCKDETSLETELKNVEVQEPRK 1200
              +E+SIP        +SVPVLKVQEAS+PV+KL RD  CKD+TSLETELKNVEVQEP+K
Sbjct: 1164 DDTEVSIPDSCPTQTADSVPVLKVQEASVPVQKLERD-GCKDDTSLETELKNVEVQEPKK 1223

Query: 1201 SSDKKTKKQKSSKLLSSDQAKDSKNSAIQQSKQSKSGKPELNDLKLKADSVVGKSSDTSS 1260
            SSDKKTKKQKSSK LSSDQAKDSKNSAIQQSKQSKSGK E NDLKLKAD+++GK+SD +S
Sbjct: 1224 SSDKKTKKQKSSKSLSSDQAKDSKNSAIQQSKQSKSGKSE-NDLKLKADNIMGKASDMAS 1283

Query: 1261 SPRKIRDA-DAKIAVVDSQPFQRSASAVNSWNDAETVQVKDDSRLTGSDSVPNSQSQSGQ 1320
            SPRKIRD  D KI++VD+QP Q SASA+N+W+D +TVQVKDD++L GSDSV NSQ+QS Q
Sbjct: 1284 SPRKIRDGDDGKISIVDNQPVQSSASAMNTWSDGDTVQVKDDAKLVGSDSVLNSQTQSSQ 1343

Query: 1321 RAWKTATSFRAKSLLEIQEEEQKMAHTEPAVSEISISVNSTSLSTPWAGIVSNLEPKASR 1380
            RAWK A+SF+ KSLLEIQEEEQK AHTE AVSEIS S+ S SLSTPWAGIVS+ +PKAS+
Sbjct: 1344 RAWKVASSFKPKSLLEIQEEEQKRAHTETAVSEISTSITSMSLSTPWAGIVSSSDPKASK 1403

Query: 1381 EIHKDLVNSEPSEKHESLLNSRSRKSQLHDLLAEDDMEKSGGVGDVRVSDSVQIASSPQV 1440
            EIHKD V SE SEKHE+LL SRSRKSQLHDLLAED+MEKS G  DVRVSDSVQIASSP+V
Sbjct: 1404 EIHKDSVISESSEKHENLLTSRSRKSQLHDLLAEDNMEKS-GASDVRVSDSVQIASSPRV 1463

Query: 1441 MTTQAESTDDNFIEAKETKKSRKKSAKAKGVGTKASASAPSADVPVASSPVEKGKISRQT 1500
            + TQAE  DDNFIEAK+TKKSRKKSAKAKGVGTK SA  PSADVPVASSP+EKGKISRQT
Sbjct: 1464 VATQAEPMDDNFIEAKDTKKSRKKSAKAKGVGTKPSAPVPSADVPVASSPIEKGKISRQT 1523

Query: 1501 QQEKDATPAIPSGPSFGDFVLWKGEAANVAPAPAWSSDSGKVHKPTSLRDIQKEQGRKLS 1560
            QQEK+A P IPSGPSFGDFVLWKGEAANVAPAPAWSSDSGKV KPTSLRDIQKEQGRK S
Sbjct: 1524 QQEKEAMPVIPSGPSFGDFVLWKGEAANVAPAPAWSSDSGKVPKPTSLRDIQKEQGRKTS 1583

Query: 1561 -SAQHSHQIPTPQKAQPTQVGRSSSTSTPSWALSASSPSKAA-SPLQNIPSQSKYGGDDD 1620
             +AQHSHQIPTPQKAQP+QVGRSSSTSTPSWALSASSPSKAA SPLQN+P+QS +GGDDD
Sbjct: 1584 AAAQHSHQIPTPQKAQPSQVGRSSSTSTPSWALSASSPSKAASSPLQNVPTQSNHGGDDD 1643

Query: 1621 LFWGPIESKQENQQVDVRQGSHSSWGNRNTPSKAAAASTGLLSRQKSSGGKADYPSSSPA 1680
            LFWGPIESK+ENQQVDVR GS ++WGNRNTP+K  AASTG+LSRQKSSGGKADY SSSPA
Sbjct: 1644 LFWGPIESKKENQQVDVRLGS-NNWGNRNTPAK--AASTGVLSRQKSSGGKADYLSSSPA 1703

Query: 1681 QSTQKGKQDPITKHSEAMGFRDWCESECVRLIGTKDTSFLEFCLKQSRSEAELLLIENLG 1740
            QS+QKGKQDP+TKHSEAMGFRDWCESECVRLIGTKDTSFLE+CLKQSRSEAELLLI+NLG
Sbjct: 1704 QSSQKGKQDPVTKHSEAMGFRDWCESECVRLIGTKDTSFLEYCLKQSRSEAELLLIQNLG 1763

Query: 1741 SFDPDHEFIDQFLNYKELLPADVLDIAFQRRNDRKVSEIATRDMNSGSAGGDLDPDVPV- 1800
            S+DPDH+FIDQFLNYKELL ADVL+IAFQ RNDRKVS IA+R++NSG+AGGDLDPDVPV 
Sbjct: 1764 SYDPDHDFIDQFLNYKELLAADVLEIAFQSRNDRKVSAIASREVNSGNAGGDLDPDVPVG 1823

Query: 1801 --GSAKGGGKKKGKKGKKVSPSVLGFNVVSNRIMMGEIQTVED 1822
              GSAK GGKKKGKKGKKVSPSVLGFNVVSNRIMMGEIQT+ED
Sbjct: 1824 RDGSAKSGGKKKGKKGKKVSPSVLGFNVVSNRIMMGEIQTIED 1855

BLAST of MS016814 vs. NCBI nr
Match: XP_008444701.1 (PREDICTED: uncharacterized protein LOC103487961 isoform X1 [Cucumis melo])

HSP 1 Score: 2936.4 bits (7611), Expect = 0.0e+00
Identity = 1557/1843 (84.48%), Postives = 1668/1843 (90.50%), Query Frame = 0

Query: 1    DVQGSENPIPLSPQWLLPKPGESKHGIGTGENHFSNPPAFRNRMDMMKASETYEDMNDMQ 60
            DVQGSENPIPLSPQWLLPKPGESKHGIGTGENHFS+ PA+ NRMDMMK SE YEDMND Q
Sbjct: 24   DVQGSENPIPLSPQWLLPKPGESKHGIGTGENHFSHQPAYGNRMDMMKGSENYEDMNDTQ 83

Query: 61   KKKEVFRPSLADAEIGRRDRWHDEERENNSSMRKDRWRDGEKELGDSRKMDRWVEDSSTR 120
            KKKEVFRPS+ D+EIGRRDRWHDEERENNSSMRKDRWRDGEKE+GD RKMDRW EDSSTR
Sbjct: 84   KKKEVFRPSVTDSEIGRRDRWHDEERENNSSMRKDRWRDGEKEMGDGRKMDRWNEDSSTR 143

Query: 121  VFRESRRGPSERWSDLSNRDNVHYDQRRESKWNTRWGPDDKETEGFREKRVDSGRDGDLH 180
            VFRESRRGPSERWSD +NRDNVHYDQRRESKWNTRWGPDDKETEGFREKRVDSGRDGDLH
Sbjct: 144  VFRESRRGPSERWSDSNNRDNVHYDQRRESKWNTRWGPDDKETEGFREKRVDSGRDGDLH 203

Query: 181  PDKNLSHVSNYGKNDRDGDHYRPWRSSASQGRGKGEPPHHQTQTPVKQVPAFSHRGRADN 240
             DKN SHVSNYGKNDRDGDHYRPWRSS++QGRGKGEPPHHQTQTP KQVPAFSHRGRADN
Sbjct: 204  LDKNFSHVSNYGKNDRDGDHYRPWRSSSAQGRGKGEPPHHQTQTPSKQVPAFSHRGRADN 263

Query: 241  TPPTFSLGRGIISSGVSSTNNIYSSSNSLGASSEKSGRDPYHYKYSRTKLLDVYRTTNLT 300
            TPPTFSLGRGIISSGV+ TN++YSS N LGASSEKSGR+P +YKYSRTKLLDV+RTTNLT
Sbjct: 264  TPPTFSLGRGIISSGVNPTNSVYSSPNYLGASSEKSGREPCYYKYSRTKLLDVFRTTNLT 323

Query: 301  LEPSLKDGFVPVPSLTLEEPLEPLALCAPSTEEMTFLKGIDKGEIISSGAPQVSKDGRNS 360
             + +LKDGFVPVP+LTL+EPLEPLALCAP+TEEMTFLKGIDKGEI+SSGAPQVSKDGRNS
Sbjct: 324  SQQTLKDGFVPVPTLTLDEPLEPLALCAPTTEEMTFLKGIDKGEIVSSGAPQVSKDGRNS 383

Query: 361  SEFMQTRRAKLGVSPSPAGSREDLPHGFDDYNDDKDDSTTKPGHINYSELSTERQMPPYH 420
            SEFMQ RR KLGVSPS  GSREDLPHGFDDYNDDKDDSTTK GH NYSE+STERQ+ PYH
Sbjct: 384  SEFMQARRTKLGVSPS-LGSREDLPHGFDDYNDDKDDSTTKLGHTNYSEVSTERQV-PYH 443

Query: 421  RPQSKIETIQEHMAHASGKFKSEAFREDDNAIRKADEVPVNRESSVKGGATVHPGSAWDA 480
            RPQSK E IQE M H SG FKSEAFREDDNA+RK DEVP NRESSVKG   +H  S WDA
Sbjct: 444  RPQSKNEAIQEQMGHTSGNFKSEAFREDDNALRKTDEVPGNRESSVKGATNIHSSSTWDA 503

Query: 481  SSLEQPLNTSVPDWRDNHNN-IGSGTPDKGWLQPPKNLNDGWGSNTATPSYPKDNPKWQT 540
            SSLEQ LNTS+PDWRDN NN I SGTPDKGW+Q  KNL+DGWGSNT TPSY KDN KWQ+
Sbjct: 504  SSLEQSLNTSLPDWRDNPNNIISSGTPDKGWVQSSKNLSDGWGSNTTTPSYAKDNSKWQS 563

Query: 541  GEESIIRRQLSGILDKEQLARKTIQSAPEDMQLHYIDPSGAIQGPFSGADIIQWFEGGYF 600
             EESIIRRQLSGILDKEQL+RKT+Q APEDMQLHYIDPSGAIQGPF GADIIQWFEGGYF
Sbjct: 564  TEESIIRRQLSGILDKEQLSRKTVQPAPEDMQLHYIDPSGAIQGPFGGADIIQWFEGGYF 623

Query: 601  GLDLPVRPVSAPNDLPFSALGDVMPHLRSKAKPPPGFSGPKQNEFGDALGNTSYGSLGKL 660
            GLDLPVRP +AP+DLPFSALGDVMPHLRSKAKPPPGFSGPKQNEF D+LGN SYGSLGKL
Sbjct: 624  GLDLPVRPTNAPSDLPFSALGDVMPHLRSKAKPPPGFSGPKQNEFADSLGNASYGSLGKL 683

Query: 661  HTGLNEIDTLRNETRHKHGSTVEAENRFLESLMSGNLGSSPLEKSAFSE---GYFGNNPN 720
            HTGLNEIDT+RNETRHKHGSTVEAENRFLESLMSGN+GSSPLEKSAFSE   GYFG NPN
Sbjct: 684  HTGLNEIDTMRNETRHKHGSTVEAENRFLESLMSGNIGSSPLEKSAFSEGVPGYFGTNPN 743

Query: 721  NLPSLGIDNGNNLFLLAKRMELERQRSLNNPYAFWPGIDATAKVSKPDVGLDDPIQQAKL 780
            +L SLG+DNGNNLFLLAKRMELERQRS++NPYAFWPGIDAT+KVSKPD+GLDDPIQQAKL
Sbjct: 744  SLSSLGMDNGNNLFLLAKRMELERQRSMSNPYAFWPGIDATSKVSKPDIGLDDPIQQAKL 803

Query: 781  LSSIMDHSRQTSHSQSADMSAILQGLSDKAPPGINDVAAWSKFASQCASDPLQSKLDLHH 840
            LSSI+DHSRQTSHSQS DMSAILQGLSDKAPPGIN+VA WSKFA   A DPLQSKLDLHH
Sbjct: 804  LSSIIDHSRQTSHSQSPDMSAILQGLSDKAPPGINEVAGWSKFAH--APDPLQSKLDLHH 863

Query: 841  DLNLSSQAPFGFQQQRLQPQPSLTNLLAQATDNPTLTPDKFLSSSLSQDPQLISKLQQQH 900
            +LNLSSQAPFGFQQQRLQPQPSLTNLLAQATDNPTLTPDKFL SSLSQDPQLISKLQQQH
Sbjct: 864  ELNLSSQAPFGFQQQRLQPQPSLTNLLAQATDNPTLTPDKFLPSSLSQDPQLISKLQQQH 923

Query: 901  LLQLHSQVPFSAQQMSLLDKLLLLKQQQKQEEQQQLLQQQQLLSQVLSEHQSRQHFGDPS 960
            LLQLHSQVPFSAQQMSLLDKLLLLKQQQKQEEQQQLLQQQQLLSQVLSEHQSRQH  DPS
Sbjct: 924  LLQLHSQVPFSAQQMSLLDKLLLLKQQQKQEEQQQLLQQQQLLSQVLSEHQSRQHLIDPS 983

Query: 961  FGQLQGAPIPIGNASIDPSQVQLSREKFQIGSQKPLNVAT----TFGNMPLQVTQGASYN 1020
            FGQLQGAPIPIGNAS DPSQVQ  REKFQIGSQKPLNV T     FGNM LQVTQGASYN
Sbjct: 984  FGQLQGAPIPIGNASTDPSQVQQPREKFQIGSQKPLNVVTDRAIPFGNMALQVTQGASYN 1043

Query: 1021 VNPEDPSLALPHQMFGNVIQQKSWNAALPEQFNDTHPKDMLPGSKVGEGPTLPGMISKSS 1080
            VNPEDPSLALPHQMFGNV QQK W   LPEQ  DT PKDMLPGS VGE    PG+ SK  
Sbjct: 1044 VNPEDPSLALPHQMFGNV-QQKGWTPGLPEQLTDTRPKDMLPGSIVGEASLFPGLTSKQI 1103

Query: 1081 EDVNLVPHSSDNNTNKVLEKASEDVPSLDATVTSLASDATIEPLPLKTAEVSVAIPPVEV 1140
            EDV+ V  SSD++T + LE+  E VP LD T TSLASDA +EPLPLKTA++SVA+ P EV
Sbjct: 1104 EDVSHVQKSSDSHTVQALEQIGEAVPRLDETATSLASDAMVEPLPLKTADISVALQPAEV 1163

Query: 1141 RISEISIP--------ESVPVLKVQEASMPVEKLGRDVVCKDETSLETELKNVEVQEPRK 1200
              +E+SIP        +SVPVLKVQEAS+PV+KL RD   KD+TSLETELKNVEVQEP+K
Sbjct: 1164 DDTEVSIPDSCSTQTADSVPVLKVQEASVPVQKLERDGY-KDDTSLETELKNVEVQEPKK 1223

Query: 1201 SSDKKTKKQKSSKLLSSDQAKDSKNSAIQQSKQSKSGKPELNDLKLKADSVVGKSSDTSS 1260
            SSDKKTKKQKSSK LSSDQAKDSKNSAIQQSKQSKSGK E NDLKLKAD+++GK+SD +S
Sbjct: 1224 SSDKKTKKQKSSKSLSSDQAKDSKNSAIQQSKQSKSGKSE-NDLKLKADNIMGKASDMAS 1283

Query: 1261 SPRKIRDA-DAKIAVVDSQPFQRSASAVNSWNDAETVQVKDDSRLTGSDSVPNSQSQSGQ 1320
            SPRKIRD  D KI++VD+QP Q SASA+N+W+D +TVQVKDD++L GSDSV NSQ+QS Q
Sbjct: 1284 SPRKIRDGDDGKISIVDNQPVQSSASAMNTWSDGDTVQVKDDAKLVGSDSVLNSQTQSSQ 1343

Query: 1321 RAWKTATSFRAKSLLEIQEEEQKMAHTEPAVSEISISVNSTSLSTPWAGIVSNLEPKASR 1380
            RAWK A+SF+ KSLLEIQEEEQK AHTE AVSEIS S+ S SLSTPWAGIVS+ +PKAS+
Sbjct: 1344 RAWKVASSFKPKSLLEIQEEEQKRAHTETAVSEISTSITSMSLSTPWAGIVSSSDPKASK 1403

Query: 1381 EIHKDLVNSEPSEKHESLLNSRSRKSQLHDLLAEDDMEKSGGVGDVRVSDSVQIASSPQV 1440
            EIHKD V SE SEKHE+LL SRSRKSQLHDLLAED+MEKS G  DVRVSDSVQIASSP+V
Sbjct: 1404 EIHKDSVISESSEKHENLLTSRSRKSQLHDLLAEDNMEKS-GASDVRVSDSVQIASSPRV 1463

Query: 1441 MTTQAESTDDNFIEAKETKKSRKKSAKAKGVGTKASASAPSADVPVASSPVEKGKISRQT 1500
            + TQAE  DDNFIEAK+TKKSRKKSAKAKGVGTK SA  PSADVPVASSP+EKGKISRQT
Sbjct: 1464 VATQAEPMDDNFIEAKDTKKSRKKSAKAKGVGTKPSAPVPSADVPVASSPIEKGKISRQT 1523

Query: 1501 QQEKDATPAIPSGPSFGDFVLWKGEAANVAPAPAWSSDSGKVHKPTSLRDIQKEQGRKLS 1560
            QQEK+A P IPSGPSFGDFVLWKGEAANVAPAPAWSSDSGKV KPTSLRDIQKEQGRK S
Sbjct: 1524 QQEKEAMPVIPSGPSFGDFVLWKGEAANVAPAPAWSSDSGKVPKPTSLRDIQKEQGRKTS 1583

Query: 1561 -SAQHSHQIPTPQKAQPTQVGRSSSTSTPSWALSASSPSKAA-SPLQNIPSQSKYGGDDD 1620
             +AQHSHQIPTPQKAQP+QVGRSSSTSTPSWALSASSPSKAA SPLQN+P+QS +GGDDD
Sbjct: 1584 AAAQHSHQIPTPQKAQPSQVGRSSSTSTPSWALSASSPSKAASSPLQNVPTQSNHGGDDD 1643

Query: 1621 LFWGPIESKQENQQVDVRQGSHSSWGNRNTPSKAAAASTGLLSRQKSSGGKADYPSSSPA 1680
            LFWGPIESK+ENQQVDVR GS ++WGNRNTP+K  AASTG+LSRQKSSGGKADY SSSPA
Sbjct: 1644 LFWGPIESKKENQQVDVRLGS-NNWGNRNTPAK--AASTGVLSRQKSSGGKADYLSSSPA 1703

Query: 1681 QSTQKGKQDPITKHSEAMGFRDWCESECVRLIGTKDTSFLEFCLKQSRSEAELLLIENLG 1740
            QS+QKGKQDP+TKHSEAMGFRDWCESECVRLIGTKDTSFLE+CLKQSRSEAELLLI+NLG
Sbjct: 1704 QSSQKGKQDPVTKHSEAMGFRDWCESECVRLIGTKDTSFLEYCLKQSRSEAELLLIQNLG 1763

Query: 1741 SFDPDHEFIDQFLNYKELLPADVLDIAFQRRNDRKVSEIATRDMNSGSAGGDLDPDVPV- 1800
            S+DPDH+FIDQFLNYKELL ADVL+IAFQ RNDRKVS IA+R++NSG+AGGDLDPDVPV 
Sbjct: 1764 SYDPDHDFIDQFLNYKELLAADVLEIAFQSRNDRKVSAIASREVNSGNAGGDLDPDVPVG 1823

Query: 1801 --GSAKGGGKKKGKKGKKVSPSVLGFNVVSNRIMMGEIQTVED 1822
              GSAK GGKKKGKKGKKVSPSVLGFNVVSNRIMMGEIQT+ED
Sbjct: 1824 RDGSAKSGGKKKGKKGKKVSPSVLGFNVVSNRIMMGEIQTIED 1855

BLAST of MS016814 vs. ExPASy Swiss-Prot
Match: Q9FMM3 (Protein ESSENTIAL FOR POTEXVIRUS ACCUMULATION 1 OS=Arabidopsis thaliana OX=3702 GN=EXA1 PE=1 SV=1)

HSP 1 Score: 1154.8 bits (2986), Expect = 0.0e+00
Identity = 822/1863 (44.12%), Postives = 1115/1863 (59.85%), Query Frame = 0

Query: 1    DVQGSENPIPLSPQWLLPKPGESKHGIGTGENHFSNPPAFRNRMDMMKASETYEDMNDMQ 60
            D+QGS+N IPLSPQWLL KPGE+K G+GTG+     P  + N  D+++ +   E+  D  
Sbjct: 28   DIQGSDNAIPLSPQWLLSKPGENKTGMGTGD-----PNQYGNHSDVVRTTGNGEETLDNL 87

Query: 61   KKKEVFRPSLADAEIGRRDRWHDEERENNSSMRKDRWRDGEKELGDSRKMDRWVEDSSTR 120
            KKK+VFRPSL DAE GRRDRW DEER+  SS+R DRWR+G+K+ GD++K+DRW  D+   
Sbjct: 88   KKKDVFRPSLLDAESGRRDRWRDEERDTLSSVRNDRWRNGDKDSGDNKKVDRW--DNVAP 147

Query: 121  VFRESRRGPSERWSDLSNRDNVHYDQRRESKWNTRWGPDDKETEGFREKRVDSGRDGDLH 180
             F E RRGP++RW+D  N+D    +QRRESKWN+RWGPDDKE E  R K  + G+DG++ 
Sbjct: 148  KFGEQRRGPNDRWTDSGNKDAAP-EQRRESKWNSRWGPDDKEAEIPRNKWDEPGKDGEII 207

Query: 181  PDKNLSHVSNYGKNDRDGDHYRPWRSSASQGRGKGEPPHHQTQTPVKQVPAFSH-RGRAD 240
             +K  S  ++      DGDHYRPWR   SQGRG+GE  H+Q+ TP KQV +FSH RGR +
Sbjct: 208  REKGPSLPTS------DGDHYRPWR--PSQGRGRGEALHNQS-TPNKQVTSFSHSRGRGE 267

Query: 241  NTPPTFSLGRGIISSGVSSTNNIYSSSNSLGASSEK--SG-RDPYHYKYSRTKLLDVYRT 300
            NT   FS GRG +S G S   +  + S+  G++S+K  SG  +P H +YSR KLLDVYR 
Sbjct: 268  NT-AIFSAGRGRMSPGGSIFTSAPNQSHPPGSASDKGESGPGEPPHLRYSRMKLLDVYRM 327

Query: 301  TNLTLEPSLKDGFVPVPSLTLEEPLEPLALCAPSTEEMTFLKGIDKGEIISSGAPQVSKD 360
             +        DGF+ VPSLT EEP +PLALCAPS++E+  L  I+KG+I+SSGAPQ SKD
Sbjct: 328  ADTECYEKFPDGFIEVPSLTSEEPTDPLALCAPSSDEVNVLDAIEKGKIVSSGAPQTSKD 387

Query: 361  ---GRNSSEFMQTRRAKLGVSPSPAGSREDLPHGFDDYNDDKDDSTTKPGHINYSELSTE 420
               GRN  EF Q RR +      PAGSRED+  G ++  D+  ++   P           
Sbjct: 388  GPTGRNPVEFSQPRRIR------PAGSREDMTFGAEESKDESGETRNYP----------- 447

Query: 421  RQMPPYHRPQSKIETIQEHMAHASGKFKSEAFREDDNAIRKADEVPVN--RESSVKGGAT 480
                                     KF+ EA  E     R+ +E PV   +E S++G A 
Sbjct: 448  -----------------------DDKFRPEASHEGYAPFRRGNEAPVRELKEPSMQGNAH 507

Query: 481  VHPGSAWDASSLEQPLNTSVPDWRDNHNNIGSGTPDKGWLQPPKNLNDGWGSNTATPSYP 540
            V   S W  SS  +  N +  DW D   +    + D  W  P  ++N   G+N   P   
Sbjct: 508  VQSASPWRQSSGGERSNRNSHDWNDPSADSRLKSSDSVWSHPKDSINHLGGNNMMLPQ-S 567

Query: 541  KDNPKWQTGEESIIRRQLSGILDKEQLARKTIQSAPEDMQLHYIDPSGAIQGPFSGADII 600
            K   +WQ  E+  +RRQ S + D+EQ  RK + S+PE++ L+Y DP G IQGPFSG+DII
Sbjct: 568  KGESRWQISEDPSLRRQPSLVFDREQEVRKLLPSSPEELSLYYKDPQGLIQGPFSGSDII 627

Query: 601  QWFEGGYFGLDLPVRPVSAPNDLPFSALGDVMPHLRSKAKPPPGFSGPKQNEFGDALGNT 660
             WFE GYFG+DL VR  SAPND PFS LGDVMPHLR+K+ PPPGF+G KQNEF DA G +
Sbjct: 628  GWFEAGYFGIDLLVRLASAPNDSPFSLLGDVMPHLRAKSGPPPGFTGAKQNEFVDAAGTS 687

Query: 661  SYGSLGKLHTGLNEIDTLRNETRHKHGSTVEAENRFLESLMSGNLGSSPLEKSAFSEGYF 720
            ++  +GK+H+G+ E D L+N+ R+KH +   AENRF+ESLMSG L +S    +   +GY 
Sbjct: 688  AFPGVGKVHSGMGETDMLQNDMRYKHVAGTVAENRFIESLMSGGLTNS----AQGVQGYG 747

Query: 721  GNNPNNLPSLGIDNGNNLFLLAKRMELERQRSLNNPYAFWPGIDATAKVSKPDVGLDDPI 780
             N+   L     D G +++LLAK++ELERQRS+ +PY++WPG ++   +   +       
Sbjct: 748  VNSSGGLSLPVTDGGADMYLLAKKLELERQRSIPSPYSYWPGRESANLMPGSE------- 807

Query: 781  QQAKLLSSIMDHSRQTSHSQSADMSAILQGLSDKAPPGIN-DVAAWSKFASQCASDPLQS 840
                   ++ ++++Q + S S+D+ +ILQG++D++ P ++  + AWS+        P+Q 
Sbjct: 808  -------NVSENAQQPTRSPSSDLLSILQGVTDRSSPAVSGPLPAWSQ--------PIQK 867

Query: 841  KLDLHHDLNLSSQAPFGFQQQRLQPQP-SLTNLLAQATDN---PTLTPDKFLSSSLSQDP 900
            + DLHH     +Q PFG QQQRL  Q   L+ LL Q  +N     L+PD  L++ LSQ+ 
Sbjct: 868  ESDLHHAKTFQTQIPFGVQQQRLPEQNLPLSGLLGQPMENNPGGMLSPDMMLAAGLSQEH 927

Query: 901  QLISKLQQQH-LLQLHSQVPFSAQ-QMSLLDKLLLLKQQQKQEEQQQLL-QQQQLLSQVL 960
            Q ++ LQQQ  LLQL++Q P SAQ Q  L++K+LLLK Q KQEEQQQLL QQQQL SQV 
Sbjct: 928  QSLNLLQQQQLLLQLNAQTPLSAQHQRLLVEKMLLLKHQHKQEEQQQLLRQQQQLYSQVF 987

Query: 961  SEHQ-SRQHFGDPSFGQLQGAPIPIGNASIDPSQVQLSREKFQIGSQKPLNVATTFGNMP 1020
            ++ Q S+Q FGDPS+GQLQ        AS+D  ++Q S++  Q+  Q  + V+     + 
Sbjct: 988  ADQQRSQQRFGDPSYGQLQ--------ASLDALRLQPSKDMSQVNQQVQVPVSHEERGIN 1047

Query: 1021 ----LQVTQGASYNV-NPEDPSLALPHQMFGNVIQQKSWNAALPEQFNDTHPKDMLPGSK 1080
                L VT   +  V + E PSL L +Q+FGNV  +      LP+Q +DTH K+      
Sbjct: 1048 LADLLPVTHATNQTVASFETPSLHLQNQLFGNVDPR----MVLPDQIDDTHKKE------ 1107

Query: 1081 VGEGPTLPGMISKSSEDVNLVPHSSDNNTNKVLEKASEDVPSLDATVTSLASDATIEPLP 1140
                       SKS  +  +    S +  N +  +     P   AT              
Sbjct: 1108 -----------SKSEYERTV----SADYVNSLYSEKPVLSPGYHAT-------------- 1167

Query: 1141 LKTAEVSVAIPPVEVRISEISIPESV--PVLKVQEASMPVEKLGRDVVCKDETSLETELK 1200
                E  V+ P  E   + ++ PE V   +L+ Q   M   K    +    ET   TE+K
Sbjct: 1168 -HNVEEPVSYPNNESSTATMTAPEIVESKLLEEQSKDMYAGKGEVSIELSGETP-ATEVK 1227

Query: 1201 NVEVQEPRKSSDKKTKKQKSSKLLSSDQAKDSKNSAIQQSKQSKSGKPELNDLKLKADSV 1260
            N +V   RK+S+KK++KQ++ +  ++D AK +  + +Q++K+ + G  + +++K K    
Sbjct: 1228 NNDVSVARKTSEKKSRKQRAKQ--AADLAKSTSRAPLQETKKPQPGSADDSEIKGKTK-- 1287

Query: 1261 VGKSSDTSSSPRKIRDADAKIAVVDSQPFQRSASAVNSWNDAETVQVKDDSRLTGSDSVP 1320
              KS+DT               ++D+      +S   + N ++     D  R   S S+ 
Sbjct: 1288 --KSADT---------------LIDNDTHLIKSSTATASNTSQMSSEVDSVRGEES-SLQ 1347

Query: 1321 NSQSQSGQRAWKTATSFRAKSLLEIQEEEQKMAHTEPAVSEISISVNSTSLSTPWAGIVS 1380
            N+++Q G RAWK A  F+ KSLLEIQ EEQ++A  E    +IS +VNS   + PWAGIV+
Sbjct: 1348 NTRTQPG-RAWKPAPGFKPKSLLEIQMEEQRVAQAEALAPKISSTVNSVGSAAPWAGIVT 1407

Query: 1381 NLEPKASREIH-KDLVNSEPSEKHESLLNSRSRKSQLHDLLAEDDMEKSGGVGDVRVSDS 1440
            N +    RE H +  +      K ES+   +++KS LHDLLA+D   KS      +  + 
Sbjct: 1408 NSDSNILRETHGESAITQTGVVKPESVPTLKAKKSHLHDLLADDVFAKSSD----KEREV 1467

Query: 1441 VQIASS----PQVMTTQAES-TDDNFIEAKETKKSRKKSAKAKGVGTKASASAPSADVPV 1500
            ++I S+     QV TT AES  DDNFI+A+ETKKSRKKSA+AK  G K +A  P+ D  +
Sbjct: 1468 MEIISNNDAFMQVTTTNAESFDDDNFIDARETKKSRKKSARAKTSGAKIAAHVPAVDTSL 1527

Query: 1501 ASSPVEKGKISR-QTQQEKDATPAIPSGPSFGDFVLWKGEAA-NVAPAPAWSSDSGKVHK 1560
             ++ VEKGK SR   QQEK+  PAIPSGPS GDFVLWKGE+  N  PA AWSS   K  K
Sbjct: 1528 QTNSVEKGKSSRILQQQEKEVLPAIPSGPSLGDFVLWKGESVNNPPPAAAWSSGPKKSTK 1587

Query: 1561 PTSLRDIQKEQGRKLSSAQH--SHQIPTPQKAQPTQVGRSSSTSTPSWALSASSPSKAAS 1620
            P+SLRDI KEQ  K++++ H     +PT QKA P Q  +  +    SW+ SASSPS+A S
Sbjct: 1588 PSSLRDIVKEQ-EKMTTSSHPPPSPVPTTQKAIPPQAHQGGA----SWSRSASSPSQAVS 1647

Query: 1621 PLQNIPSQSKYGGDDDLFWGPIE-SKQENQQVDV-RQGSHSSWGNRNTPSKAAAASTGLL 1680
                  SQSK  GDDDLFWGP+E S Q+ +Q D     S +SWG +NTP K  A ++  L
Sbjct: 1648 ---QSSSQSKSKGDDDLFWGPVEQSTQDTKQGDFPHLTSQNSWGTKNTPGKVNAGTS--L 1707

Query: 1681 SRQKS-SGGKADYPSSSP--AQSTQKGKQDPITKHSEAMGFRDWCESECVRLIGTKDTSF 1740
            +RQKS S G AD   SSP   Q++ KGK++ +TK +EA GFRDWC+SEC+RL+G++DTS 
Sbjct: 1708 NRQKSVSMGSADRVLSSPVVTQASHKGKKEAVTKLTEANGFRDWCKSECLRLLGSEDTSV 1714

Query: 1741 LEFCLKQSRSEAELLLIENLGSFDPDHEFIDQFLNYKELLPADVLDIAFQRRNDRKVSEI 1800
            LEFCLK SRSEAE LLIENLGS DPDH+FID+FLNYK+LLP++V++IAFQ     K S +
Sbjct: 1768 LEFCLKLSRSEAETLLIENLGSRDPDHKFIDKFLNYKDLLPSEVVEIAFQ----SKGSGV 1714

Query: 1801 ATRDMNSGSAGGDLDPDVPVGSAKGGGKKKGKKGKKV--SPSVLGFNVVSNRIMMGEIQT 1822
             TR+ N+G            G +K GGKKK KKGKKV  S SVLGFNVVSNRIMMGEIQT
Sbjct: 1828 GTRN-NTGEDYYYNTTAANDGFSKVGGKKKAKKGKKVSLSASVLGFNVVSNRIMMGEIQT 1714

BLAST of MS016814 vs. ExPASy Swiss-Prot
Match: Q6Y7W6 (GRB10-interacting GYF protein 2 OS=Homo sapiens OX=9606 GN=GIGYF2 PE=1 SV=1)

HSP 1 Score: 53.9 bits (128), Expect = 2.2e-05
Identity = 41/130 (31.54%), Postives = 57/130 (43.85%), Query Frame = 0

Query: 520 GWGSNTATPSYPKDNPKWQTGEESIIRRQLSGILDKEQLARK---------TIQSAPEDM 579
           G GS +  P   +     +   E ++       LD E+LA K         +I    E M
Sbjct: 474 GMGSVSTEPDDEEGLKHLEQQAEKMVAYLQDSALDDERLASKLQEHRAKGVSIPLMHEAM 533

Query: 580 Q-LHYIDPSGAIQGPFSGADIIQWFEGGYFGLDLPVRPVSAPNDLPFSALGDVM------ 634
           Q  +Y DP G IQGPF+  ++ +WF+ GYF + L V+      D  F  LGD+M      
Sbjct: 534 QKWYYKDPQGEIQGPFNNQEMAEWFQAGYFTMSLLVKRAC---DESFQPLGDIMKMWGRV 593

BLAST of MS016814 vs. ExPASy Swiss-Prot
Match: Q5U236 (GRB10-interacting GYF protein 2 OS=Xenopus laevis OX=8355 GN=gigyf2 PE=2 SV=1)

HSP 1 Score: 53.9 bits (128), Expect = 2.2e-05
Identity = 34/102 (33.33%), Postives = 48/102 (47.06%), Query Frame = 0

Query: 551 GILDKEQLARKTIQ------SAPEDMQLHYIDPSGAIQGPFSGADIIQWFEGGYFGLDLP 610
           G LD + L  K +       S     + +Y DP G IQGPFS  ++ +W++ GYF + L 
Sbjct: 464 GTLDDDHLLTKVLDQRVKGPSLDNQQKWYYKDPQGEIQGPFSNREMAEWYQAGYFPMTLL 523

Query: 611 VRPVSAPNDLPFSALGDVMPHLRSKAKPPPGFSGPKQNEFGD 647
           +R V    D  F  LGD+    +   + P  FS P     GD
Sbjct: 524 LRRVC---DETFQPLGDI---FKKWGRVP--FSTPPTPRLGD 557

BLAST of MS016814 vs. ExPASy TrEMBL
Match: A0A6J1CT22 (uncharacterized protein LOC111014032 OS=Momordica charantia OX=3673 GN=LOC111014032 PE=4 SV=1)

HSP 1 Score: 3484.5 bits (9034), Expect = 0.0e+00
Identity = 1812/1821 (99.51%), Postives = 1813/1821 (99.56%), Query Frame = 0

Query: 1    DVQGSENPIPLSPQWLLPKPGESKHGIGTGENHFSNPPAFRNRMDMMKASETYEDMNDMQ 60
            DVQGSENPIPLSPQWLLPKPGESKHGIGTGENHFSNPPAFRNRMDMMKASE YED NDMQ
Sbjct: 24   DVQGSENPIPLSPQWLLPKPGESKHGIGTGENHFSNPPAFRNRMDMMKASENYEDTNDMQ 83

Query: 61   KKKEVFRPSLADAEIGRRDRWHDEERENNSSMRKDRWRDGEKELGDSRKMDRWVEDSSTR 120
            KKKEVFRPSLADAEIGRRDRWHDEERENNSSMRKDRWRDGEKELGDSRKMDRWVEDSSTR
Sbjct: 84   KKKEVFRPSLADAEIGRRDRWHDEERENNSSMRKDRWRDGEKELGDSRKMDRWVEDSSTR 143

Query: 121  VFRESRRGPSERWSDLSNRDNVHYDQRRESKWNTRWGPDDKETEGFREKRVDSGRDGDLH 180
            VFRESRRGPSERWSDLSNRDNVHYDQRRESKWNTRWGPDDKETEGFREKRVDSGRDGDLH
Sbjct: 144  VFRESRRGPSERWSDLSNRDNVHYDQRRESKWNTRWGPDDKETEGFREKRVDSGRDGDLH 203

Query: 181  PDKNLSHVSNYGKNDRDGDHYRPWRSSASQGRGKGEPPHHQTQTPVKQVPAFSHRGRADN 240
            PDKNLSHVSNYGKNDRDGDHYRPWRSSASQGRGKGEPPHHQTQTPVKQVPAFSHRGRADN
Sbjct: 204  PDKNLSHVSNYGKNDRDGDHYRPWRSSASQGRGKGEPPHHQTQTPVKQVPAFSHRGRADN 263

Query: 241  TPPTFSLGRGIISSGVSSTNNIYSSSNSLGASSEKSGRDPYHYKYSRTKLLDVYRTTNLT 300
             PPTFSLGRGIISSGVSSTNNIYSSSNSLGASSEKSGRDPYHYKYSRTKLLDVYRTTNLT
Sbjct: 264  IPPTFSLGRGIISSGVSSTNNIYSSSNSLGASSEKSGRDPYHYKYSRTKLLDVYRTTNLT 323

Query: 301  LEPSLKDGFVPVPSLTLEEPLEPLALCAPSTEEMTFLKGIDKGEIISSGAPQVSKDGRNS 360
            LEPSLKDGFVPVPSLTLEEPLEPLALCAPSTEEMTFLKGIDKGEIISSGAPQVSKDGRNS
Sbjct: 324  LEPSLKDGFVPVPSLTLEEPLEPLALCAPSTEEMTFLKGIDKGEIISSGAPQVSKDGRNS 383

Query: 361  SEFMQTRRAKLGVSPSPAGSREDLPHGFDDYNDDKDDSTTKPGHINYSELSTERQMPPYH 420
            SEFMQTRRAKLGVSPSP GSREDLPHGFDDYNDDKDDSTTKPGHINYSELSTERQMPPYH
Sbjct: 384  SEFMQTRRAKLGVSPSP-GSREDLPHGFDDYNDDKDDSTTKPGHINYSELSTERQMPPYH 443

Query: 421  RPQSKIETIQEHMAHASGKFKSEAFREDDNAIRKADEVPVNRESSVKGGATVHPGSAWDA 480
            RPQSKIETIQEHMAHASGKFKSEAFREDDNAIRKADEVPVNRESSVKGGATVHPGSAWDA
Sbjct: 444  RPQSKIETIQEHMAHASGKFKSEAFREDDNAIRKADEVPVNRESSVKGGATVHPGSAWDA 503

Query: 481  SSLEQPLNTSVPDWRDNHNNIGSGTPDKGWLQPPKNLNDGWGSNTATPSYPKDNPKWQTG 540
            SSLEQPLNTSVPDWRDNHNNIGSGTPDKGWLQPPKNLNDGWGSNTATPSYPKDNPKWQTG
Sbjct: 504  SSLEQPLNTSVPDWRDNHNNIGSGTPDKGWLQPPKNLNDGWGSNTATPSYPKDNPKWQTG 563

Query: 541  EESIIRRQLSGILDKEQLARKTIQSAPEDMQLHYIDPSGAIQGPFSGADIIQWFEGGYFG 600
            EESIIRRQLSGILDKEQLARKTIQSAPEDMQLHYIDPSGAIQGPFSGADIIQWFEGGYFG
Sbjct: 564  EESIIRRQLSGILDKEQLARKTIQSAPEDMQLHYIDPSGAIQGPFSGADIIQWFEGGYFG 623

Query: 601  LDLPVRPVSAPNDLPFSALGDVMPHLRSKAKPPPGFSGPKQNEFGDALGNTSYGSLGKLH 660
            LDLPVRPVSAPNDLPFSALGDVMPHLRSKAKPPPGFSGPKQNEFGDALGNTSYGSLGKLH
Sbjct: 624  LDLPVRPVSAPNDLPFSALGDVMPHLRSKAKPPPGFSGPKQNEFGDALGNTSYGSLGKLH 683

Query: 661  TGLNEIDTLRNETRHKHGSTVEAENRFLESLMSGNLGSSPLEKSAFSEGYFGNNPNNLPS 720
            TGLNEIDTLRNET HKHGSTVEAENRFLESLMSGNLGSSPLEKSAFSEGYFGNNPNNLPS
Sbjct: 684  TGLNEIDTLRNETTHKHGSTVEAENRFLESLMSGNLGSSPLEKSAFSEGYFGNNPNNLPS 743

Query: 721  LGIDNGNNLFLLAKRMELERQRSLNNPYAFWPGIDATAKVSKPDVGLDDPIQQAKLLSSI 780
            LGIDNGNNLFLLAKRMELERQRSLNNPYAFWPGIDATAKVSKPDVGLDDPIQQAKLLSSI
Sbjct: 744  LGIDNGNNLFLLAKRMELERQRSLNNPYAFWPGIDATAKVSKPDVGLDDPIQQAKLLSSI 803

Query: 781  MDHSRQTSHSQSADMSAILQGLSDKAPPGINDVAAWSKFASQCASDPLQSKLDLHHDLNL 840
            MDHSRQTSHSQSADMSAILQGLSDKAPPGINDVAAWSKFASQCASDPLQSKLDLHHDLNL
Sbjct: 804  MDHSRQTSHSQSADMSAILQGLSDKAPPGINDVAAWSKFASQCASDPLQSKLDLHHDLNL 863

Query: 841  SSQAPFGFQQQRLQPQPSLTNLLAQATDNPTLTPDKFLSSSLSQDPQLISKLQQQHLLQL 900
            SSQAPFGFQQQRLQPQPSLTNLLAQATDNPTLTPDKFLSSSLSQDPQLISKLQQQHLLQL
Sbjct: 864  SSQAPFGFQQQRLQPQPSLTNLLAQATDNPTLTPDKFLSSSLSQDPQLISKLQQQHLLQL 923

Query: 901  HSQVPFSAQQMSLLDKLLLLKQQQKQEEQQQLLQQQQLLSQVLSEHQSRQHFGDPSFGQL 960
            HSQVPFSAQQMSLLDKLLLLKQQQKQEEQQQLLQQQQLLSQVLSEHQSRQHFGDPSFGQL
Sbjct: 924  HSQVPFSAQQMSLLDKLLLLKQQQKQEEQQQLLQQQQLLSQVLSEHQSRQHFGDPSFGQL 983

Query: 961  QGAPIPIGNASIDPSQVQLSREKFQIGSQKPLNVATTFGNMPLQVTQGASYNVNPEDPSL 1020
            QGAPIPIGNASIDPSQVQLSREKFQIGSQKPLNVATTFGNMPLQVTQGASYNVNPEDPSL
Sbjct: 984  QGAPIPIGNASIDPSQVQLSREKFQIGSQKPLNVATTFGNMPLQVTQGASYNVNPEDPSL 1043

Query: 1021 ALPHQMFGNVIQQKSWNAALPEQFNDTHPKDMLPGSKVGEGPTLPGMISKSSEDVNLVPH 1080
            ALPHQMFGNVIQQKSWNAALPEQFNDTHPKDMLPGSKVGEGPTLPGMISKSSEDVNLVPH
Sbjct: 1044 ALPHQMFGNVIQQKSWNAALPEQFNDTHPKDMLPGSKVGEGPTLPGMISKSSEDVNLVPH 1103

Query: 1081 SSDNNTNKVLEKASEDVPSLDATVTSLASDATIEPLPLKTAEVSVAIPPVEVRISEISIP 1140
            SSDNNTNKVLEKASEDVPSLDATVTSLASDATIEPLPLKTAEVSVAIPPVEVRISEISIP
Sbjct: 1104 SSDNNTNKVLEKASEDVPSLDATVTSLASDATIEPLPLKTAEVSVAIPPVEVRISEISIP 1163

Query: 1141 ESVPVLKVQEASMPVEKLGRDVVCKDETSLETELKNVEVQEPRKSSDKKTKKQKSSKLLS 1200
            ESVPVLKVQEASMPVEKLGRDVVCKDET LETELKNVEVQEPRKSSDKKTKKQKSSKLLS
Sbjct: 1164 ESVPVLKVQEASMPVEKLGRDVVCKDET-LETELKNVEVQEPRKSSDKKTKKQKSSKLLS 1223

Query: 1201 SDQAKDSKNSAIQQSKQSKSGKPELNDLKLKADSVVGKSSDTSSSPRKIRDADAKIAVVD 1260
            SDQAKDSKNSAIQQSKQSKSGKPELNDLKLKADSVVGKSSDTSSSPRKIRDADAKIAVVD
Sbjct: 1224 SDQAKDSKNSAIQQSKQSKSGKPELNDLKLKADSVVGKSSDTSSSPRKIRDADAKIAVVD 1283

Query: 1261 SQPFQRSASAVNSWNDAETVQVKDDSRLTGSDSVPNSQSQSGQRAWKTATSFRAKSLLEI 1320
            SQPFQ SASAVNSWNDAETVQVKDDSRLTGSDSVPNSQSQSGQRAWKTATSFRAKSLLEI
Sbjct: 1284 SQPFQSSASAVNSWNDAETVQVKDDSRLTGSDSVPNSQSQSGQRAWKTATSFRAKSLLEI 1343

Query: 1321 QEEEQKMAHTEPAVSEISISVNSTSLSTPWAGIVSNLEPKASREIHKDLVNSEPSEKHES 1380
            QEEEQKMAHTEPAVSEISISVNSTSLSTPWAGIVSNL+PKASREIHKD VNSEPSEKHES
Sbjct: 1344 QEEEQKMAHTEPAVSEISISVNSTSLSTPWAGIVSNLDPKASREIHKDFVNSEPSEKHES 1403

Query: 1381 LLNSRSRKSQLHDLLAEDDMEKSGGVGDVRVSDSVQIASSPQVMTTQAESTDDNFIEAKE 1440
            LLNSRSRKSQLHDLLAEDDMEKSGGVGDVRVSDSVQIASSPQVMTTQAESTDDNFIEAKE
Sbjct: 1404 LLNSRSRKSQLHDLLAEDDMEKSGGVGDVRVSDSVQIASSPQVMTTQAESTDDNFIEAKE 1463

Query: 1441 TKKSRKKSAKAKGVGTKASASAPSADVPVASSPVEKGKISRQTQQEKDATPAIPSGPSFG 1500
            TKKSRKKSAKAKGVGTKASASAPSADVPVASSPVEKGKISRQTQQEKDATPAIPSGPSFG
Sbjct: 1464 TKKSRKKSAKAKGVGTKASASAPSADVPVASSPVEKGKISRQTQQEKDATPAIPSGPSFG 1523

Query: 1501 DFVLWKGEAANVAPAPAWSSDSGKVHKPTSLRDIQKEQGRKLSSAQHSHQIPTPQKAQPT 1560
            DFVLWKGEAANVAPAPAWSSDSGKVHKPTSLRDIQKEQGRKLSSAQHSHQIPTPQKAQPT
Sbjct: 1524 DFVLWKGEAANVAPAPAWSSDSGKVHKPTSLRDIQKEQGRKLSSAQHSHQIPTPQKAQPT 1583

Query: 1561 QVGRSSSTSTPSWALSASSPSKAASPLQNIPSQSKYGGDDDLFWGPIESKQENQQVDVRQ 1620
            QVGRSSSTSTPSWALSASSPSKAASPLQNIPSQSKYGGDDDLFWGPIESKQENQQVDVRQ
Sbjct: 1584 QVGRSSSTSTPSWALSASSPSKAASPLQNIPSQSKYGGDDDLFWGPIESKQENQQVDVRQ 1643

Query: 1621 GSHSSWGNRNTPSKAAAASTGLLSRQKSSGGKADYPSSSPAQSTQKGKQDPITKHSEAMG 1680
            GSHSSWGNRNTPSKAAAASTGLLSRQKSSGGKADYPSSSPAQSTQKGKQDPITKHSEAMG
Sbjct: 1644 GSHSSWGNRNTPSKAAAASTGLLSRQKSSGGKADYPSSSPAQSTQKGKQDPITKHSEAMG 1703

Query: 1681 FRDWCESECVRLIGTKDTSFLEFCLKQSRSEAELLLIENLGSFDPDHEFIDQFLNYKELL 1740
            FRDWCESECVRLIGTKDTSFLEFCLKQSRSEAELLLIENLGSFDPDHEFIDQFLNYKELL
Sbjct: 1704 FRDWCESECVRLIGTKDTSFLEFCLKQSRSEAELLLIENLGSFDPDHEFIDQFLNYKELL 1763

Query: 1741 PADVLDIAFQRRNDRKVSEIATRDMNSGSAGGDLDPDVPVGSAKGGGKKKGKKGKKVSPS 1800
            PADVLDIAFQRRNDRKVSEIATRDMNSGSAGGDLDPDVPVGSAKGGGKKKGKKGKKVSPS
Sbjct: 1764 PADVLDIAFQRRNDRKVSEIATRDMNSGSAGGDLDPDVPVGSAKGGGKKKGKKGKKVSPS 1823

Query: 1801 VLGFNVVSNRIMMGEIQTVED 1822
            VLGFNVVSNRIMMGEIQTVED
Sbjct: 1824 VLGFNVVSNRIMMGEIQTVED 1842

BLAST of MS016814 vs. ExPASy TrEMBL
Match: A0A5A7VAQ0 (GYF domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G005840 PE=4 SV=1)

HSP 1 Score: 2940.2 bits (7621), Expect = 0.0e+00
Identity = 1558/1843 (84.54%), Postives = 1669/1843 (90.56%), Query Frame = 0

Query: 1    DVQGSENPIPLSPQWLLPKPGESKHGIGTGENHFSNPPAFRNRMDMMKASETYEDMNDMQ 60
            DVQGSENPIPLSPQWLLPKPGESKHGIGTGENHFS+ PA+ NRMDMMK SE YEDMND Q
Sbjct: 24   DVQGSENPIPLSPQWLLPKPGESKHGIGTGENHFSHQPAYGNRMDMMKGSENYEDMNDTQ 83

Query: 61   KKKEVFRPSLADAEIGRRDRWHDEERENNSSMRKDRWRDGEKELGDSRKMDRWVEDSSTR 120
            KKKEVFRPS+ D+EIGRRDRWHDEERENNSSMRKDRWRDGEKE+GD RKMDRW EDSSTR
Sbjct: 84   KKKEVFRPSVTDSEIGRRDRWHDEERENNSSMRKDRWRDGEKEMGDGRKMDRWNEDSSTR 143

Query: 121  VFRESRRGPSERWSDLSNRDNVHYDQRRESKWNTRWGPDDKETEGFREKRVDSGRDGDLH 180
            VFRESRRGPSERWSD +NRDNVHYDQRRESKWNTRWGPDDKETEGFREKRVDSGRDGDLH
Sbjct: 144  VFRESRRGPSERWSDSNNRDNVHYDQRRESKWNTRWGPDDKETEGFREKRVDSGRDGDLH 203

Query: 181  PDKNLSHVSNYGKNDRDGDHYRPWRSSASQGRGKGEPPHHQTQTPVKQVPAFSHRGRADN 240
             DKN SHVSNYGKNDRDGDHYRPWRSS++QGRGKGEPPHHQTQTP KQVPAFSHRGRADN
Sbjct: 204  LDKNFSHVSNYGKNDRDGDHYRPWRSSSAQGRGKGEPPHHQTQTPSKQVPAFSHRGRADN 263

Query: 241  TPPTFSLGRGIISSGVSSTNNIYSSSNSLGASSEKSGRDPYHYKYSRTKLLDVYRTTNLT 300
            TPPTFSLGRGIISSGV+ TN++YSS N LGASSEKSGR+P +YKYSRTKLLDV+RTTNLT
Sbjct: 264  TPPTFSLGRGIISSGVNPTNSVYSSPNYLGASSEKSGREPCYYKYSRTKLLDVFRTTNLT 323

Query: 301  LEPSLKDGFVPVPSLTLEEPLEPLALCAPSTEEMTFLKGIDKGEIISSGAPQVSKDGRNS 360
             + +LKDGFVPVP+LTL+EPLEPLALCAP+TEEMTFLKGIDKGEI+SSGAPQVSKDGRNS
Sbjct: 324  SQQTLKDGFVPVPTLTLDEPLEPLALCAPTTEEMTFLKGIDKGEIVSSGAPQVSKDGRNS 383

Query: 361  SEFMQTRRAKLGVSPSPAGSREDLPHGFDDYNDDKDDSTTKPGHINYSELSTERQMPPYH 420
            SEFMQ RR KLGVSPS  GSREDLPHGFDDYNDDKDDSTTK GH NYSE+STERQ+ PYH
Sbjct: 384  SEFMQARRTKLGVSPS-LGSREDLPHGFDDYNDDKDDSTTKLGHTNYSEVSTERQV-PYH 443

Query: 421  RPQSKIETIQEHMAHASGKFKSEAFREDDNAIRKADEVPVNRESSVKGGATVHPGSAWDA 480
            RPQSK E IQE M H SG FKSEAFREDDNA+RK DEVP NRESSVKG   +H  S WDA
Sbjct: 444  RPQSKNEAIQEQMGHTSGNFKSEAFREDDNALRKTDEVPGNRESSVKGATNIHSSSTWDA 503

Query: 481  SSLEQPLNTSVPDWRDNHNN-IGSGTPDKGWLQPPKNLNDGWGSNTATPSYPKDNPKWQT 540
            SSLEQ LNTS+PDWRDN NN I SGTPDKGW+Q  KNL+DGWGSNT TPSY KDN KWQ+
Sbjct: 504  SSLEQSLNTSLPDWRDNPNNIISSGTPDKGWVQSSKNLSDGWGSNTTTPSYAKDNSKWQS 563

Query: 541  GEESIIRRQLSGILDKEQLARKTIQSAPEDMQLHYIDPSGAIQGPFSGADIIQWFEGGYF 600
             EESIIRRQLSGILDKEQL+RKT+Q APEDMQLHYIDPSGAIQGPF GADIIQWFEGGYF
Sbjct: 564  TEESIIRRQLSGILDKEQLSRKTVQPAPEDMQLHYIDPSGAIQGPFGGADIIQWFEGGYF 623

Query: 601  GLDLPVRPVSAPNDLPFSALGDVMPHLRSKAKPPPGFSGPKQNEFGDALGNTSYGSLGKL 660
            GLDLPVRP +AP+DLPFSALGDVMPHLRSKAKPPPGFSGPKQNEF D+LGN SYGSLGKL
Sbjct: 624  GLDLPVRPTNAPSDLPFSALGDVMPHLRSKAKPPPGFSGPKQNEFADSLGNASYGSLGKL 683

Query: 661  HTGLNEIDTLRNETRHKHGSTVEAENRFLESLMSGNLGSSPLEKSAFSE---GYFGNNPN 720
            HTGLNEIDT+RNETRHKHGSTVEAENRFLESLMSGN+GSSPLEKSAFSE   GYFG NPN
Sbjct: 684  HTGLNEIDTMRNETRHKHGSTVEAENRFLESLMSGNIGSSPLEKSAFSEGVPGYFGTNPN 743

Query: 721  NLPSLGIDNGNNLFLLAKRMELERQRSLNNPYAFWPGIDATAKVSKPDVGLDDPIQQAKL 780
            +L SLG+DNGNNLFLLAKRMELERQRS++NPYAFWPGIDAT+KVSKPD+GLDDPIQQAKL
Sbjct: 744  SLSSLGMDNGNNLFLLAKRMELERQRSMSNPYAFWPGIDATSKVSKPDIGLDDPIQQAKL 803

Query: 781  LSSIMDHSRQTSHSQSADMSAILQGLSDKAPPGINDVAAWSKFASQCASDPLQSKLDLHH 840
            LSSI+DHSRQTSHSQS DMSAILQGLSDKAPPGIN+VA WSKFA   A DPLQSKLDLHH
Sbjct: 804  LSSIIDHSRQTSHSQSPDMSAILQGLSDKAPPGINEVAGWSKFAH--APDPLQSKLDLHH 863

Query: 841  DLNLSSQAPFGFQQQRLQPQPSLTNLLAQATDNPTLTPDKFLSSSLSQDPQLISKLQQQH 900
            +LNLSSQAPFGFQQQRLQPQPSLTNLLAQATDNPTLTPDKFL SSLSQDPQLISKLQQQH
Sbjct: 864  ELNLSSQAPFGFQQQRLQPQPSLTNLLAQATDNPTLTPDKFLPSSLSQDPQLISKLQQQH 923

Query: 901  LLQLHSQVPFSAQQMSLLDKLLLLKQQQKQEEQQQLLQQQQLLSQVLSEHQSRQHFGDPS 960
            LLQLHSQVPFSAQQMSLLDKLLLLKQQQKQEEQQQLLQQQQLLSQVLSEHQSRQH  DPS
Sbjct: 924  LLQLHSQVPFSAQQMSLLDKLLLLKQQQKQEEQQQLLQQQQLLSQVLSEHQSRQHLIDPS 983

Query: 961  FGQLQGAPIPIGNASIDPSQVQLSREKFQIGSQKPLNVAT----TFGNMPLQVTQGASYN 1020
            FGQLQGAPIPIGNAS DPSQVQ  REKFQIGSQKPLNV T     FGNM LQVTQGASYN
Sbjct: 984  FGQLQGAPIPIGNASTDPSQVQQPREKFQIGSQKPLNVVTDRAIPFGNMALQVTQGASYN 1043

Query: 1021 VNPEDPSLALPHQMFGNVIQQKSWNAALPEQFNDTHPKDMLPGSKVGEGPTLPGMISKSS 1080
            VNPEDPSLALPHQMFGNV QQK W   LPEQ  DT PKDMLPGS VGE    PG+ SK  
Sbjct: 1044 VNPEDPSLALPHQMFGNV-QQKGWTPGLPEQLTDTRPKDMLPGSIVGEASLFPGLTSKQI 1103

Query: 1081 EDVNLVPHSSDNNTNKVLEKASEDVPSLDATVTSLASDATIEPLPLKTAEVSVAIPPVEV 1140
            EDV+ V  SSD++T + LE+  E VP LD T TSLASDA +EPLPLKTA++SVA+ P EV
Sbjct: 1104 EDVSHVQKSSDSHTVQALEQIGEAVPRLDETATSLASDAMVEPLPLKTADISVALQPAEV 1163

Query: 1141 RISEISIP--------ESVPVLKVQEASMPVEKLGRDVVCKDETSLETELKNVEVQEPRK 1200
              +E+SIP        +SVPVLKVQEAS+PV+KL RD  CKD+TSLETELKNVEVQEP+K
Sbjct: 1164 DDTEVSIPDSCPTQTADSVPVLKVQEASVPVQKLERD-GCKDDTSLETELKNVEVQEPKK 1223

Query: 1201 SSDKKTKKQKSSKLLSSDQAKDSKNSAIQQSKQSKSGKPELNDLKLKADSVVGKSSDTSS 1260
            SSDKKTKKQKSSK LSSDQAKDSKNSAIQQSKQSKSGK E NDLKLKAD+++GK+SD +S
Sbjct: 1224 SSDKKTKKQKSSKSLSSDQAKDSKNSAIQQSKQSKSGKSE-NDLKLKADNIMGKASDMAS 1283

Query: 1261 SPRKIRDA-DAKIAVVDSQPFQRSASAVNSWNDAETVQVKDDSRLTGSDSVPNSQSQSGQ 1320
            SPRKIRD  D KI++VD+QP Q SASA+N+W+D +TVQVKDD++L GSDSV NSQ+QS Q
Sbjct: 1284 SPRKIRDGDDGKISIVDNQPVQSSASAMNTWSDGDTVQVKDDAKLVGSDSVLNSQTQSSQ 1343

Query: 1321 RAWKTATSFRAKSLLEIQEEEQKMAHTEPAVSEISISVNSTSLSTPWAGIVSNLEPKASR 1380
            RAWK A+SF+ KSLLEIQEEEQK AHTE AVSEIS S+ S SLSTPWAGIVS+ +PKAS+
Sbjct: 1344 RAWKVASSFKPKSLLEIQEEEQKRAHTETAVSEISTSITSMSLSTPWAGIVSSSDPKASK 1403

Query: 1381 EIHKDLVNSEPSEKHESLLNSRSRKSQLHDLLAEDDMEKSGGVGDVRVSDSVQIASSPQV 1440
            EIHKD V SE SEKHE+LL SRSRKSQLHDLLAED+MEKS G  DVRVSDSVQIASSP+V
Sbjct: 1404 EIHKDSVISESSEKHENLLTSRSRKSQLHDLLAEDNMEKS-GASDVRVSDSVQIASSPRV 1463

Query: 1441 MTTQAESTDDNFIEAKETKKSRKKSAKAKGVGTKASASAPSADVPVASSPVEKGKISRQT 1500
            + TQAE  DDNFIEAK+TKKSRKKSAKAKGVGTK SA  PSADVPVASSP+EKGKISRQT
Sbjct: 1464 VATQAEPMDDNFIEAKDTKKSRKKSAKAKGVGTKPSAPVPSADVPVASSPIEKGKISRQT 1523

Query: 1501 QQEKDATPAIPSGPSFGDFVLWKGEAANVAPAPAWSSDSGKVHKPTSLRDIQKEQGRKLS 1560
            QQEK+A P IPSGPSFGDFVLWKGEAANVAPAPAWSSDSGKV KPTSLRDIQKEQGRK S
Sbjct: 1524 QQEKEAMPVIPSGPSFGDFVLWKGEAANVAPAPAWSSDSGKVPKPTSLRDIQKEQGRKTS 1583

Query: 1561 -SAQHSHQIPTPQKAQPTQVGRSSSTSTPSWALSASSPSKAA-SPLQNIPSQSKYGGDDD 1620
             +AQHSHQIPTPQKAQP+QVGRSSSTSTPSWALSASSPSKAA SPLQN+P+QS +GGDDD
Sbjct: 1584 AAAQHSHQIPTPQKAQPSQVGRSSSTSTPSWALSASSPSKAASSPLQNVPTQSNHGGDDD 1643

Query: 1621 LFWGPIESKQENQQVDVRQGSHSSWGNRNTPSKAAAASTGLLSRQKSSGGKADYPSSSPA 1680
            LFWGPIESK+ENQQVDVR GS ++WGNRNTP+K  AASTG+LSRQKSSGGKADY SSSPA
Sbjct: 1644 LFWGPIESKKENQQVDVRLGS-NNWGNRNTPAK--AASTGVLSRQKSSGGKADYLSSSPA 1703

Query: 1681 QSTQKGKQDPITKHSEAMGFRDWCESECVRLIGTKDTSFLEFCLKQSRSEAELLLIENLG 1740
            QS+QKGKQDP+TKHSEAMGFRDWCESECVRLIGTKDTSFLE+CLKQSRSEAELLLI+NLG
Sbjct: 1704 QSSQKGKQDPVTKHSEAMGFRDWCESECVRLIGTKDTSFLEYCLKQSRSEAELLLIQNLG 1763

Query: 1741 SFDPDHEFIDQFLNYKELLPADVLDIAFQRRNDRKVSEIATRDMNSGSAGGDLDPDVPV- 1800
            S+DPDH+FIDQFLNYKELL ADVL+IAFQ RNDRKVS IA+R++NSG+AGGDLDPDVPV 
Sbjct: 1764 SYDPDHDFIDQFLNYKELLAADVLEIAFQSRNDRKVSAIASREVNSGNAGGDLDPDVPVG 1823

Query: 1801 --GSAKGGGKKKGKKGKKVSPSVLGFNVVSNRIMMGEIQTVED 1822
              GSAK GGKKKGKKGKKVSPSVLGFNVVSNRIMMGEIQT+ED
Sbjct: 1824 RDGSAKSGGKKKGKKGKKVSPSVLGFNVVSNRIMMGEIQTIED 1855

BLAST of MS016814 vs. ExPASy TrEMBL
Match: A0A1S3BBQ1 (uncharacterized protein LOC103487961 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103487961 PE=4 SV=1)

HSP 1 Score: 2936.4 bits (7611), Expect = 0.0e+00
Identity = 1557/1843 (84.48%), Postives = 1668/1843 (90.50%), Query Frame = 0

Query: 1    DVQGSENPIPLSPQWLLPKPGESKHGIGTGENHFSNPPAFRNRMDMMKASETYEDMNDMQ 60
            DVQGSENPIPLSPQWLLPKPGESKHGIGTGENHFS+ PA+ NRMDMMK SE YEDMND Q
Sbjct: 24   DVQGSENPIPLSPQWLLPKPGESKHGIGTGENHFSHQPAYGNRMDMMKGSENYEDMNDTQ 83

Query: 61   KKKEVFRPSLADAEIGRRDRWHDEERENNSSMRKDRWRDGEKELGDSRKMDRWVEDSSTR 120
            KKKEVFRPS+ D+EIGRRDRWHDEERENNSSMRKDRWRDGEKE+GD RKMDRW EDSSTR
Sbjct: 84   KKKEVFRPSVTDSEIGRRDRWHDEERENNSSMRKDRWRDGEKEMGDGRKMDRWNEDSSTR 143

Query: 121  VFRESRRGPSERWSDLSNRDNVHYDQRRESKWNTRWGPDDKETEGFREKRVDSGRDGDLH 180
            VFRESRRGPSERWSD +NRDNVHYDQRRESKWNTRWGPDDKETEGFREKRVDSGRDGDLH
Sbjct: 144  VFRESRRGPSERWSDSNNRDNVHYDQRRESKWNTRWGPDDKETEGFREKRVDSGRDGDLH 203

Query: 181  PDKNLSHVSNYGKNDRDGDHYRPWRSSASQGRGKGEPPHHQTQTPVKQVPAFSHRGRADN 240
             DKN SHVSNYGKNDRDGDHYRPWRSS++QGRGKGEPPHHQTQTP KQVPAFSHRGRADN
Sbjct: 204  LDKNFSHVSNYGKNDRDGDHYRPWRSSSAQGRGKGEPPHHQTQTPSKQVPAFSHRGRADN 263

Query: 241  TPPTFSLGRGIISSGVSSTNNIYSSSNSLGASSEKSGRDPYHYKYSRTKLLDVYRTTNLT 300
            TPPTFSLGRGIISSGV+ TN++YSS N LGASSEKSGR+P +YKYSRTKLLDV+RTTNLT
Sbjct: 264  TPPTFSLGRGIISSGVNPTNSVYSSPNYLGASSEKSGREPCYYKYSRTKLLDVFRTTNLT 323

Query: 301  LEPSLKDGFVPVPSLTLEEPLEPLALCAPSTEEMTFLKGIDKGEIISSGAPQVSKDGRNS 360
             + +LKDGFVPVP+LTL+EPLEPLALCAP+TEEMTFLKGIDKGEI+SSGAPQVSKDGRNS
Sbjct: 324  SQQTLKDGFVPVPTLTLDEPLEPLALCAPTTEEMTFLKGIDKGEIVSSGAPQVSKDGRNS 383

Query: 361  SEFMQTRRAKLGVSPSPAGSREDLPHGFDDYNDDKDDSTTKPGHINYSELSTERQMPPYH 420
            SEFMQ RR KLGVSPS  GSREDLPHGFDDYNDDKDDSTTK GH NYSE+STERQ+ PYH
Sbjct: 384  SEFMQARRTKLGVSPS-LGSREDLPHGFDDYNDDKDDSTTKLGHTNYSEVSTERQV-PYH 443

Query: 421  RPQSKIETIQEHMAHASGKFKSEAFREDDNAIRKADEVPVNRESSVKGGATVHPGSAWDA 480
            RPQSK E IQE M H SG FKSEAFREDDNA+RK DEVP NRESSVKG   +H  S WDA
Sbjct: 444  RPQSKNEAIQEQMGHTSGNFKSEAFREDDNALRKTDEVPGNRESSVKGATNIHSSSTWDA 503

Query: 481  SSLEQPLNTSVPDWRDNHNN-IGSGTPDKGWLQPPKNLNDGWGSNTATPSYPKDNPKWQT 540
            SSLEQ LNTS+PDWRDN NN I SGTPDKGW+Q  KNL+DGWGSNT TPSY KDN KWQ+
Sbjct: 504  SSLEQSLNTSLPDWRDNPNNIISSGTPDKGWVQSSKNLSDGWGSNTTTPSYAKDNSKWQS 563

Query: 541  GEESIIRRQLSGILDKEQLARKTIQSAPEDMQLHYIDPSGAIQGPFSGADIIQWFEGGYF 600
             EESIIRRQLSGILDKEQL+RKT+Q APEDMQLHYIDPSGAIQGPF GADIIQWFEGGYF
Sbjct: 564  TEESIIRRQLSGILDKEQLSRKTVQPAPEDMQLHYIDPSGAIQGPFGGADIIQWFEGGYF 623

Query: 601  GLDLPVRPVSAPNDLPFSALGDVMPHLRSKAKPPPGFSGPKQNEFGDALGNTSYGSLGKL 660
            GLDLPVRP +AP+DLPFSALGDVMPHLRSKAKPPPGFSGPKQNEF D+LGN SYGSLGKL
Sbjct: 624  GLDLPVRPTNAPSDLPFSALGDVMPHLRSKAKPPPGFSGPKQNEFADSLGNASYGSLGKL 683

Query: 661  HTGLNEIDTLRNETRHKHGSTVEAENRFLESLMSGNLGSSPLEKSAFSE---GYFGNNPN 720
            HTGLNEIDT+RNETRHKHGSTVEAENRFLESLMSGN+GSSPLEKSAFSE   GYFG NPN
Sbjct: 684  HTGLNEIDTMRNETRHKHGSTVEAENRFLESLMSGNIGSSPLEKSAFSEGVPGYFGTNPN 743

Query: 721  NLPSLGIDNGNNLFLLAKRMELERQRSLNNPYAFWPGIDATAKVSKPDVGLDDPIQQAKL 780
            +L SLG+DNGNNLFLLAKRMELERQRS++NPYAFWPGIDAT+KVSKPD+GLDDPIQQAKL
Sbjct: 744  SLSSLGMDNGNNLFLLAKRMELERQRSMSNPYAFWPGIDATSKVSKPDIGLDDPIQQAKL 803

Query: 781  LSSIMDHSRQTSHSQSADMSAILQGLSDKAPPGINDVAAWSKFASQCASDPLQSKLDLHH 840
            LSSI+DHSRQTSHSQS DMSAILQGLSDKAPPGIN+VA WSKFA   A DPLQSKLDLHH
Sbjct: 804  LSSIIDHSRQTSHSQSPDMSAILQGLSDKAPPGINEVAGWSKFAH--APDPLQSKLDLHH 863

Query: 841  DLNLSSQAPFGFQQQRLQPQPSLTNLLAQATDNPTLTPDKFLSSSLSQDPQLISKLQQQH 900
            +LNLSSQAPFGFQQQRLQPQPSLTNLLAQATDNPTLTPDKFL SSLSQDPQLISKLQQQH
Sbjct: 864  ELNLSSQAPFGFQQQRLQPQPSLTNLLAQATDNPTLTPDKFLPSSLSQDPQLISKLQQQH 923

Query: 901  LLQLHSQVPFSAQQMSLLDKLLLLKQQQKQEEQQQLLQQQQLLSQVLSEHQSRQHFGDPS 960
            LLQLHSQVPFSAQQMSLLDKLLLLKQQQKQEEQQQLLQQQQLLSQVLSEHQSRQH  DPS
Sbjct: 924  LLQLHSQVPFSAQQMSLLDKLLLLKQQQKQEEQQQLLQQQQLLSQVLSEHQSRQHLIDPS 983

Query: 961  FGQLQGAPIPIGNASIDPSQVQLSREKFQIGSQKPLNVAT----TFGNMPLQVTQGASYN 1020
            FGQLQGAPIPIGNAS DPSQVQ  REKFQIGSQKPLNV T     FGNM LQVTQGASYN
Sbjct: 984  FGQLQGAPIPIGNASTDPSQVQQPREKFQIGSQKPLNVVTDRAIPFGNMALQVTQGASYN 1043

Query: 1021 VNPEDPSLALPHQMFGNVIQQKSWNAALPEQFNDTHPKDMLPGSKVGEGPTLPGMISKSS 1080
            VNPEDPSLALPHQMFGNV QQK W   LPEQ  DT PKDMLPGS VGE    PG+ SK  
Sbjct: 1044 VNPEDPSLALPHQMFGNV-QQKGWTPGLPEQLTDTRPKDMLPGSIVGEASLFPGLTSKQI 1103

Query: 1081 EDVNLVPHSSDNNTNKVLEKASEDVPSLDATVTSLASDATIEPLPLKTAEVSVAIPPVEV 1140
            EDV+ V  SSD++T + LE+  E VP LD T TSLASDA +EPLPLKTA++SVA+ P EV
Sbjct: 1104 EDVSHVQKSSDSHTVQALEQIGEAVPRLDETATSLASDAMVEPLPLKTADISVALQPAEV 1163

Query: 1141 RISEISIP--------ESVPVLKVQEASMPVEKLGRDVVCKDETSLETELKNVEVQEPRK 1200
              +E+SIP        +SVPVLKVQEAS+PV+KL RD   KD+TSLETELKNVEVQEP+K
Sbjct: 1164 DDTEVSIPDSCSTQTADSVPVLKVQEASVPVQKLERDGY-KDDTSLETELKNVEVQEPKK 1223

Query: 1201 SSDKKTKKQKSSKLLSSDQAKDSKNSAIQQSKQSKSGKPELNDLKLKADSVVGKSSDTSS 1260
            SSDKKTKKQKSSK LSSDQAKDSKNSAIQQSKQSKSGK E NDLKLKAD+++GK+SD +S
Sbjct: 1224 SSDKKTKKQKSSKSLSSDQAKDSKNSAIQQSKQSKSGKSE-NDLKLKADNIMGKASDMAS 1283

Query: 1261 SPRKIRDA-DAKIAVVDSQPFQRSASAVNSWNDAETVQVKDDSRLTGSDSVPNSQSQSGQ 1320
            SPRKIRD  D KI++VD+QP Q SASA+N+W+D +TVQVKDD++L GSDSV NSQ+QS Q
Sbjct: 1284 SPRKIRDGDDGKISIVDNQPVQSSASAMNTWSDGDTVQVKDDAKLVGSDSVLNSQTQSSQ 1343

Query: 1321 RAWKTATSFRAKSLLEIQEEEQKMAHTEPAVSEISISVNSTSLSTPWAGIVSNLEPKASR 1380
            RAWK A+SF+ KSLLEIQEEEQK AHTE AVSEIS S+ S SLSTPWAGIVS+ +PKAS+
Sbjct: 1344 RAWKVASSFKPKSLLEIQEEEQKRAHTETAVSEISTSITSMSLSTPWAGIVSSSDPKASK 1403

Query: 1381 EIHKDLVNSEPSEKHESLLNSRSRKSQLHDLLAEDDMEKSGGVGDVRVSDSVQIASSPQV 1440
            EIHKD V SE SEKHE+LL SRSRKSQLHDLLAED+MEKS G  DVRVSDSVQIASSP+V
Sbjct: 1404 EIHKDSVISESSEKHENLLTSRSRKSQLHDLLAEDNMEKS-GASDVRVSDSVQIASSPRV 1463

Query: 1441 MTTQAESTDDNFIEAKETKKSRKKSAKAKGVGTKASASAPSADVPVASSPVEKGKISRQT 1500
            + TQAE  DDNFIEAK+TKKSRKKSAKAKGVGTK SA  PSADVPVASSP+EKGKISRQT
Sbjct: 1464 VATQAEPMDDNFIEAKDTKKSRKKSAKAKGVGTKPSAPVPSADVPVASSPIEKGKISRQT 1523

Query: 1501 QQEKDATPAIPSGPSFGDFVLWKGEAANVAPAPAWSSDSGKVHKPTSLRDIQKEQGRKLS 1560
            QQEK+A P IPSGPSFGDFVLWKGEAANVAPAPAWSSDSGKV KPTSLRDIQKEQGRK S
Sbjct: 1524 QQEKEAMPVIPSGPSFGDFVLWKGEAANVAPAPAWSSDSGKVPKPTSLRDIQKEQGRKTS 1583

Query: 1561 -SAQHSHQIPTPQKAQPTQVGRSSSTSTPSWALSASSPSKAA-SPLQNIPSQSKYGGDDD 1620
             +AQHSHQIPTPQKAQP+QVGRSSSTSTPSWALSASSPSKAA SPLQN+P+QS +GGDDD
Sbjct: 1584 AAAQHSHQIPTPQKAQPSQVGRSSSTSTPSWALSASSPSKAASSPLQNVPTQSNHGGDDD 1643

Query: 1621 LFWGPIESKQENQQVDVRQGSHSSWGNRNTPSKAAAASTGLLSRQKSSGGKADYPSSSPA 1680
            LFWGPIESK+ENQQVDVR GS ++WGNRNTP+K  AASTG+LSRQKSSGGKADY SSSPA
Sbjct: 1644 LFWGPIESKKENQQVDVRLGS-NNWGNRNTPAK--AASTGVLSRQKSSGGKADYLSSSPA 1703

Query: 1681 QSTQKGKQDPITKHSEAMGFRDWCESECVRLIGTKDTSFLEFCLKQSRSEAELLLIENLG 1740
            QS+QKGKQDP+TKHSEAMGFRDWCESECVRLIGTKDTSFLE+CLKQSRSEAELLLI+NLG
Sbjct: 1704 QSSQKGKQDPVTKHSEAMGFRDWCESECVRLIGTKDTSFLEYCLKQSRSEAELLLIQNLG 1763

Query: 1741 SFDPDHEFIDQFLNYKELLPADVLDIAFQRRNDRKVSEIATRDMNSGSAGGDLDPDVPV- 1800
            S+DPDH+FIDQFLNYKELL ADVL+IAFQ RNDRKVS IA+R++NSG+AGGDLDPDVPV 
Sbjct: 1764 SYDPDHDFIDQFLNYKELLAADVLEIAFQSRNDRKVSAIASREVNSGNAGGDLDPDVPVG 1823

Query: 1801 --GSAKGGGKKKGKKGKKVSPSVLGFNVVSNRIMMGEIQTVED 1822
              GSAK GGKKKGKKGKKVSPSVLGFNVVSNRIMMGEIQT+ED
Sbjct: 1824 RDGSAKSGGKKKGKKGKKVSPSVLGFNVVSNRIMMGEIQTIED 1855

BLAST of MS016814 vs. ExPASy TrEMBL
Match: A0A0A0LRG9 (GYF domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G361560 PE=4 SV=1)

HSP 1 Score: 2921.3 bits (7572), Expect = 0.0e+00
Identity = 1549/1835 (84.41%), Postives = 1655/1835 (90.19%), Query Frame = 0

Query: 1    DVQGSENPIPLSPQWLLPKPGESKHGIGTGENHFSNPPAFRNRMDMMKASETYEDMNDMQ 60
            DVQGSENPIPLSPQWLLPKPGESKHGIGTGENHFS+ PA+ NRMDMMK SE YEDMND Q
Sbjct: 24   DVQGSENPIPLSPQWLLPKPGESKHGIGTGENHFSHQPAYGNRMDMMKGSENYEDMNDTQ 83

Query: 61   KKKEVFRPSLADAEIGRRDRWHDEERENNSSMRKDRWRDGEKELGDSRKMDRWVEDSSTR 120
            KKKEVFRPSL D+E GRRDRWHDEERENNSSMRKDRWRDGEKE+GDSRKMDRW EDSSTR
Sbjct: 84   KKKEVFRPSLTDSETGRRDRWHDEERENNSSMRKDRWRDGEKEMGDSRKMDRWNEDSSTR 143

Query: 121  VFRESRRGPSERWSDLSNRDNVHYDQRRESKWNTRWGPDDKETEGFREKRVDSGRDGDLH 180
            VFRESRRGPSERWSD +NRDNVHYDQRRESKWNTRWGPDDKETEGFREKRVDSGRDGDLH
Sbjct: 144  VFRESRRGPSERWSDSNNRDNVHYDQRRESKWNTRWGPDDKETEGFREKRVDSGRDGDLH 203

Query: 181  PDKNLSHVSNYGKNDRDGDHYRPWRSSASQGRGKGEPPHHQTQTPVKQVPAFSHRGRADN 240
             DKN SHVSNYGKNDRDGDHYRPWRSS++QGRGKGE PHHQTQTP KQVPAFSHRGRADN
Sbjct: 204  LDKNFSHVSNYGKNDRDGDHYRPWRSSSAQGRGKGELPHHQTQTPSKQVPAFSHRGRADN 263

Query: 241  TPPTFSLGRGIISSGVSSTNNIYSSSNSLGASSEKSGRDPYHYKYSRTKLLDVYRTTNLT 300
            TPPTFSLGRGIISSGV+ TN+IYSS N LGASSEKSGR+PY+YKYSRTKLLDV+RTTNLT
Sbjct: 264  TPPTFSLGRGIISSGVNPTNSIYSSPNYLGASSEKSGREPYYYKYSRTKLLDVFRTTNLT 323

Query: 301  LEPSLKDGFVPVPSLTLEEPLEPLALCAPSTEEMTFLKGIDKGEIISSGAPQVSKDGRNS 360
             + +LKD FVPVP+LTL+EPLEPLALCAP+TEEMTFLKGIDKGEI+SSGAPQVSKDGRNS
Sbjct: 324  SQQTLKDVFVPVPTLTLDEPLEPLALCAPTTEEMTFLKGIDKGEIVSSGAPQVSKDGRNS 383

Query: 361  SEFMQTRRAKLGVSPSPAGSREDLPHGFDDYNDDKDDSTTKPGHINYSELSTERQMPPYH 420
            SEFMQ RR KLGVSPS  GSREDLPHGFDDYNDDKDDSTTK GH NYSE+STERQ+ PYH
Sbjct: 384  SEFMQARRTKLGVSPS-LGSREDLPHGFDDYNDDKDDSTTKLGHTNYSEVSTERQV-PYH 443

Query: 421  RPQSKIETIQEHMAHASGKFKSEAFREDDNAIRKADEVPVNRESSVKGGATVHPGSAWDA 480
            RPQSK E IQE M H SG FKSEAFREDDNA+RK DEVP NRESSVKGG  +HP S WDA
Sbjct: 444  RPQSKNEAIQEQMGHTSGTFKSEAFREDDNALRKTDEVPGNRESSVKGGTNIHPSSTWDA 503

Query: 481  SSLEQPLNTSVPDWRDNHNN-IGSGTPDKGWLQPPKNLNDGWGSNTATPSYPKDNPKWQT 540
            SSLEQPLNTS+PDWRDN NN I SGTPDKGW+Q  KNLNDGWGSN   PSY KDN KWQT
Sbjct: 504  SSLEQPLNTSLPDWRDNPNNIISSGTPDKGWVQSSKNLNDGWGSNATNPSYAKDNSKWQT 563

Query: 541  GEESIIRRQLSGILDKEQLARKTIQSAPEDMQLHYIDPSGAIQGPFSGADIIQWFEGGYF 600
             EESI+RRQLSGILDKEQL+RKT+Q A ED+QLHYIDPSGAIQGPF GADIIQWFEGGYF
Sbjct: 564  AEESILRRQLSGILDKEQLSRKTVQPAAEDLQLHYIDPSGAIQGPFGGADIIQWFEGGYF 623

Query: 601  GLDLPVRPVSAPNDLPFSALGDVMPHLRSKAKPPPGFSGPKQNEFGDALGNTSYGSLGKL 660
            GLDLPVRP +AP+DLPFSALGDVMPHLRSKAKPPPGFSGPKQNEF D+LGN S+GSLGKL
Sbjct: 624  GLDLPVRPTNAPSDLPFSALGDVMPHLRSKAKPPPGFSGPKQNEFADSLGNPSFGSLGKL 683

Query: 661  HTGLNEIDTLRNETRHKHGSTVEAENRFLESLMSGNLGSSPLEKSAFSE---GYFGNNPN 720
            HTGLNEIDTLRNETRHKHGSTVEAENRFLESLMSGN+GSSPLEKSAFSE   GYFGNNPN
Sbjct: 684  HTGLNEIDTLRNETRHKHGSTVEAENRFLESLMSGNIGSSPLEKSAFSEGVPGYFGNNPN 743

Query: 721  NLPSLGIDNGNNLFLLAKRMELERQRSLNNPYAFWPGIDATAKVSKPDVGLDDPIQQAKL 780
            +L SLGIDNGNNLFLLAKRMELERQRSL+NPYAFWPGIDAT+KVSKPD+GLDDPIQQAKL
Sbjct: 744  SLSSLGIDNGNNLFLLAKRMELERQRSLSNPYAFWPGIDATSKVSKPDIGLDDPIQQAKL 803

Query: 781  LSSIMDHSRQTSHSQSADMSAILQGLSDKAPPGINDVAAWSKFASQCASDPLQSKLDLHH 840
            LSSI+DHSRQTSHSQS DMSAILQGLSDKAPPGIN+VA WSKF+   A DPLQSKLDLHH
Sbjct: 804  LSSIIDHSRQTSHSQSPDMSAILQGLSDKAPPGINEVAGWSKFSH--APDPLQSKLDLHH 863

Query: 841  DLNLSSQAPFGFQQQRLQPQPSLTNLLAQATDNPTLTPDKFLSSSLSQDPQLISKLQQQH 900
            DLNL SQAPFGFQQQRLQPQPSLTNLLAQATDNPTLTPDKFL SSLSQDPQLISKLQQQH
Sbjct: 864  DLNLPSQAPFGFQQQRLQPQPSLTNLLAQATDNPTLTPDKFLPSSLSQDPQLISKLQQQH 923

Query: 901  LLQLHSQVPFSAQQMSLLDKLLLLKQQQKQEEQQQLLQQQQLLSQVLSEHQSRQHFGDPS 960
            LLQLHSQVPFSAQQMSLLDKLLLLKQQQKQEEQQQLLQQQQLLSQVLSEHQSRQH  DPS
Sbjct: 924  LLQLHSQVPFSAQQMSLLDKLLLLKQQQKQEEQQQLLQQQQLLSQVLSEHQSRQHLIDPS 983

Query: 961  FGQLQGAPIPIGNASIDPSQVQLSREKFQIGSQKPLNVAT----TFGNMPLQVTQGASYN 1020
            FGQLQGAPIPIGNAS DPSQVQ  REKFQIGSQKPLNV T     FGNM LQVTQGASYN
Sbjct: 984  FGQLQGAPIPIGNASADPSQVQQPREKFQIGSQKPLNVVTDRAIPFGNMALQVTQGASYN 1043

Query: 1021 VNPEDPSLALPHQMFGNVIQQKSWNAALPEQFNDTHPKDMLPGSKVGEGPTLPGMISKSS 1080
            VN EDPSLALPHQMFGNV QQK W   LPEQ  DT  KDMLPGS VGE    PG+ SK S
Sbjct: 1044 VNSEDPSLALPHQMFGNV-QQKGWTPGLPEQLTDTRSKDMLPGSIVGEVSLFPGLTSKPS 1103

Query: 1081 EDVNLVPHSSDNNTNKVLEKASEDVPSLDATVTSLASDATIEPLPLKTAEVSVAIPPVEV 1140
            EDV+ V  SSD++T + LE+  EDVP LDAT TSLASD  +EPLPLKTA++SVA+ P EV
Sbjct: 1104 EDVSHVQKSSDSHTIQALEQIGEDVPRLDATATSLASDVMVEPLPLKTADISVALQPAEV 1163

Query: 1141 RISEISIPESVPVLKVQEASMPVEKLGRDVVCKDETSLETELKNVEVQEPRKSSDKKTKK 1200
               E+SIP+SVPVLKVQEASMPV+KL R   CKD+T+LETELKN+EVQEP+K SDKKTKK
Sbjct: 1164 HDIEVSIPDSVPVLKVQEASMPVQKLERG-GCKDDTTLETELKNIEVQEPKKPSDKKTKK 1223

Query: 1201 QKSSKLLSSDQAKDSKNSAIQQSKQSKSGKPELNDLKLKADSVVGKSSDTSSSPRKIRDA 1260
            QKSSK LSSDQAKDSKNSAIQQSKQSKSGK E NDLKLKAD+++GKSSD +SSPRKIRD 
Sbjct: 1224 QKSSKSLSSDQAKDSKNSAIQQSKQSKSGKSE-NDLKLKADNIMGKSSDLASSPRKIRDG 1283

Query: 1261 -DAKIAVVDSQPFQRSASAVNSWNDAETVQVKDDSRLTGSDSVPNSQSQSGQRAWKTATS 1320
             D KI+VVD QP Q SASA+N+W+D +TVQVKDD+RL GSDSV NSQ+QS QRAWK A+S
Sbjct: 1284 DDGKISVVDHQPIQSSASAMNTWSDGDTVQVKDDARLVGSDSVLNSQTQSAQRAWKVASS 1343

Query: 1321 FRAKSLLEIQEEEQKMAHTEPAVSEISISVNSTSLSTPWAGIVSNLEPKASREIHKDLVN 1380
            F+ KSLLEIQEEEQK AHTE AVSEIS S+ S SLSTPWAGIVS+ +PKAS+EIHKD V 
Sbjct: 1344 FKPKSLLEIQEEEQKRAHTETAVSEISTSITSMSLSTPWAGIVSSSDPKASKEIHKDSVI 1403

Query: 1381 SEPSEKHESLLNSRSRKSQLHDLLAEDDMEKSGGVGDVRVSDSVQIASSPQVMTTQAEST 1440
            SE SEKHE+LL S+ R+SQLHDLLAED+MEKS G  DVRVSDSVQIASSP+V+ TQAE  
Sbjct: 1404 SESSEKHENLLISKIRRSQLHDLLAEDNMEKS-GASDVRVSDSVQIASSPRVLATQAEPM 1463

Query: 1441 DDNFIEAKETKKSRKKSAKAKGVGTKASASAPSADVPVASSPVEKGKISRQTQQEKDATP 1500
            DDNFIEAK+TKKSRKKSAKAKGVG+K SA  PS DVPV SSP EKGKISRQTQQEK+A P
Sbjct: 1464 DDNFIEAKDTKKSRKKSAKAKGVGSKPSAPVPSGDVPVGSSPNEKGKISRQTQQEKEAMP 1523

Query: 1501 AIPSGPSFGDFVLWKGEAANVAPAPAW-SSDSGKVHKPTSLRDIQKEQGRKLSSAQHSHQ 1560
            AIPSGPSFGDFVLWKGE ANVAP+PAW SSDSGKV KPTSLRDIQKEQGRK S+AQHSHQ
Sbjct: 1524 AIPSGPSFGDFVLWKGEVANVAPSPAWSSSDSGKVPKPTSLRDIQKEQGRKTSAAQHSHQ 1583

Query: 1561 IPTPQKAQPTQVGRSSSTSTPSWALSASSPSKAA-SPLQNIPSQSKYGGDDDLFWGPIES 1620
            IPTPQK QP+QVGRSSSTSTPSWALSASSPSKAA SPLQN+P+QS +GGDDDLFWGPIES
Sbjct: 1584 IPTPQKGQPSQVGRSSSTSTPSWALSASSPSKAASSPLQNVPTQSNHGGDDDLFWGPIES 1643

Query: 1621 KQENQQVDVRQGSHSSWGNRNTPSKAAAASTGLLSRQKSSGGKADYPSSSPAQSTQKGKQ 1680
            K+ENQQVDVR  S ++WGNRN P+K  AASTG+LSRQKSSGGKADY SSSPAQS+QKGKQ
Sbjct: 1644 KKENQQVDVRLVS-NNWGNRNAPAK--AASTGVLSRQKSSGGKADYLSSSPAQSSQKGKQ 1703

Query: 1681 DPITKHSEAMGFRDWCESECVRLIGTKDTSFLEFCLKQSRSEAELLLIENLGSFDPDHEF 1740
            DP+TKHSEAMGFRDWCESEC RLIG KDTSFLEFCLKQSRSEAEL LIENLGS+DPDH+F
Sbjct: 1704 DPVTKHSEAMGFRDWCESECERLIGIKDTSFLEFCLKQSRSEAELYLIENLGSYDPDHDF 1763

Query: 1741 IDQFLNYKELLPADVLDIAFQRRNDRKVSEIATRDMNSGSAGGDLDPDVPV---GSAKGG 1800
            IDQFLNYK+LLPADVL+IAFQ RNDRKVS +A+R++NSG+AGGDLDPDVPV   GSAK G
Sbjct: 1764 IDQFLNYKDLLPADVLEIAFQSRNDRKVSAVASREVNSGNAGGDLDPDVPVGRDGSAKSG 1823

Query: 1801 GKKKGKKGKKVSPSVLGFNVVSNRIMMGEIQTVED 1822
            GKKKGKKGKKV+PSVLGFNVVSNRIMMGEIQTVED
Sbjct: 1824 GKKKGKKGKKVNPSVLGFNVVSNRIMMGEIQTVED 1847

BLAST of MS016814 vs. ExPASy TrEMBL
Match: A0A6J1K6T8 (uncharacterized protein LOC111491700 OS=Cucurbita maxima OX=3661 GN=LOC111491700 PE=4 SV=1)

HSP 1 Score: 2915.2 bits (7556), Expect = 0.0e+00
Identity = 1549/1838 (84.28%), Postives = 1660/1838 (90.32%), Query Frame = 0

Query: 1    DVQGSENPIPLSPQWLLPKPGESKHGIGTGENHFSNPPAFRNRMDMMKASETYEDMNDMQ 60
            DVQGSENPIPLSPQWLLPKPGESKHG+GTGENHFS+ P + NRMD MK SE  EDMN++Q
Sbjct: 24   DVQGSENPIPLSPQWLLPKPGESKHGMGTGENHFSHQPVYGNRMDTMKGSENSEDMNEIQ 83

Query: 61   KKKEVFRPSLADAEIGRRDRWHDEERENNSSMRKDRWRDGEKELGDSRKMDRWVEDSSTR 120
            KKKE+FRPSL D+EIGRRDRWHDEEREN+SS+RKDRWRDGEKE+GD RKMDRW EDSSTR
Sbjct: 84   KKKEIFRPSLTDSEIGRRDRWHDEERENSSSVRKDRWRDGEKEIGDGRKMDRWNEDSSTR 143

Query: 121  VFRESRRGPSERWSDLSNRDNVHYDQRRESKWNTRWGPDDKETEGFREKRVDSGRDGDLH 180
            VFRESRRGPSERWSD SNRDNVHYDQRRESKWNTRWGPDDKETEGFREKRVDSGRDGDLH
Sbjct: 144  VFRESRRGPSERWSDSSNRDNVHYDQRRESKWNTRWGPDDKETEGFREKRVDSGRDGDLH 203

Query: 181  PDKNLSHVSNYGKNDRDGDHYRPWRSSASQGRGKGEPPHHQTQTPVKQVPAFSHRGRADN 240
             DKN SHVSNYGKNDRDGDHYRPWRSS+SQGRGKGEPPHHQTQTP+KQVPAFSHRGRADN
Sbjct: 204  LDKNFSHVSNYGKNDRDGDHYRPWRSSSSQGRGKGEPPHHQTQTPIKQVPAFSHRGRADN 263

Query: 241  TPPTFSLGRGIISSGVSSTNNIYSSSNSLGASSEKSGRDPYHYKYSRTKLLDVYRTTNLT 300
            TPPTFSLGRGIISSGV+  N+IYSS +SLGASSEKSGR+PY+YKYSRTKLLDV+RTT+LT
Sbjct: 264  TPPTFSLGRGIISSGVNPPNSIYSSPHSLGASSEKSGREPYYYKYSRTKLLDVFRTTSLT 323

Query: 301  LEPSLKDGFVPVPSLTLEEPLEPLALCAPSTEEMTFLKGIDKGEIISSGAPQVSKDGRNS 360
             + +LKDGFVPVP+LTL+EPLEPLALC P+TEEMTFLKGIDKGEI+SSGAPQVSKDGRNS
Sbjct: 324  SQQTLKDGFVPVPTLTLDEPLEPLALCVPTTEEMTFLKGIDKGEIVSSGAPQVSKDGRNS 383

Query: 361  SEFMQTRRAKLGVSPSPAGSREDLPHGFDDYNDDKDDSTTKPGHINYSELSTERQMPPYH 420
            SEFMQTRR KLGVSPS  GSREDLPHGFDD NDDKDDSTTKPGH NYSE++TERQ+ PYH
Sbjct: 384  SEFMQTRRTKLGVSPS-LGSREDLPHGFDDCNDDKDDSTTKPGHTNYSEVTTERQL-PYH 443

Query: 421  RPQSKIETIQEHMAHASGKFKSEAFREDDNAIRKADEVPVNRESSVKGGATVHPGSAWDA 480
            RP          +AHAS  FKSEAFREDDNA+RKADE PV+RESSVKGG  V  G  WDA
Sbjct: 444  RP----------LAHASSTFKSEAFREDDNAMRKADEGPVSRESSVKGGTNVQHGRTWDA 503

Query: 481  SSLEQPLNTSVPDWRDNHNNIGSGTPDKGWLQPPKNLNDGWGSNTATPSYPKDNPKWQTG 540
            SSLEQ LNTS+PDWRDN NNI SGTPDKG LQ  KNLNDGWGSN+ATPSYPK+NPKW TG
Sbjct: 504  SSLEQLLNTSLPDWRDNPNNINSGTPDKG-LQSSKNLNDGWGSNSATPSYPKENPKWHTG 563

Query: 541  EESIIRRQLSGILDKEQLARKTIQSAPEDMQLHYIDPSGAIQGPFSGADIIQWFEGGYFG 600
            +ESI+RRQLSGILDKEQLARKT+QSAPED+Q HYIDPSGAIQGPFSGADIIQWFEGGYFG
Sbjct: 564  DESILRRQLSGILDKEQLARKTVQSAPEDLQFHYIDPSGAIQGPFSGADIIQWFEGGYFG 623

Query: 601  LDLPVRPVSAPNDLPFSALGDVMPHLRSKAKPPPGFSGPKQNEFGDALGNTSYGSLGKLH 660
            LDLPVR  +APN+LPFSALGD MPHLRSKAKPPPGF GPKQNEF D LGN S GSLGKLH
Sbjct: 624  LDLPVRLANAPNELPFSALGDAMPHLRSKAKPPPGFCGPKQNEFADTLGNASIGSLGKLH 683

Query: 661  TGLNEIDTLRNETRHKHGSTVEAENRFLESLMSGNLGSSPLEKSAFSE---GYFGNNPNN 720
            TGLNEID LRNETRHKHGSTVEAENRFLESLMSGN+GSSPLEKSAFSE   GYFGNN +N
Sbjct: 684  TGLNEIDPLRNETRHKHGSTVEAENRFLESLMSGNIGSSPLEKSAFSEGLPGYFGNNSSN 743

Query: 721  LPSLGIDNGNNLFLLAKRMELERQRSLNNPYAFWPGIDATAKVSKPDVGLDDPIQQAKLL 780
            LPSLGIDNGNNLFLLAKRMELERQRSL+NPYAFWPGIDA++KVSKPD+GLDDPIQQAKLL
Sbjct: 744  LPSLGIDNGNNLFLLAKRMELERQRSLSNPYAFWPGIDASSKVSKPDIGLDDPIQQAKLL 803

Query: 781  SSIMDHSRQTSHSQSADMSAILQGLSDKAPPGINDVAAWSKFASQCASDPLQSKLDLHHD 840
            SSI+DHSRQTSH QSADMSAILQGLSDKAPPGINDVA WSKFASQCA DPLQSKLDLHHD
Sbjct: 804  SSIIDHSRQTSHPQSADMSAILQGLSDKAPPGINDVAGWSKFASQCAPDPLQSKLDLHHD 863

Query: 841  LNLSSQAPFGFQQQRLQPQPSLTNLLAQATDNPTLTPDKFLSSSLSQDPQLISKLQQQHL 900
            LNLSSQAPFGFQQQRLQPQP LTNLLAQATDN TLT DKFL SSLSQDPQLISKLQQQHL
Sbjct: 864  LNLSSQAPFGFQQQRLQPQPPLTNLLAQATDNSTLTSDKFLPSSLSQDPQLISKLQQQHL 923

Query: 901  LQLHSQVPFSAQQMSLLDKLLLLKQQQKQEEQQQLL--QQQQLLSQVLSEHQSRQHFGDP 960
            LQLHSQVPFSAQQMSLLDK+LLLKQQQKQEEQQQLL  QQQQLLSQVLSEHQS QHF DP
Sbjct: 924  LQLHSQVPFSAQQMSLLDKILLLKQQQKQEEQQQLLQQQQQQLLSQVLSEHQSHQHFVDP 983

Query: 961  SFGQLQGAPIPIGNASIDPSQVQLSREKFQIGSQKPLNV----ATTFGNMPLQVTQGASY 1020
            SFGQL GA IPIGNAS DPSQVQLSREKFQIGSQKPLNV    ATT+GNM LQ TQGASY
Sbjct: 984  SFGQLHGASIPIGNASNDPSQVQLSREKFQIGSQKPLNVLTDHATTYGNMALQATQGASY 1043

Query: 1021 NVNPEDPSLALPHQMFGNVIQQKSWNAALPEQFNDTHPKDMLPGSKVGEGPTLPGMISKS 1080
            NVN EDPSL LP QMFGNV+QQKSW AA+PEQ NDT PKD++PGS V EG  LPGM SKS
Sbjct: 1044 NVNSEDPSLILPPQMFGNVVQQKSWTAAIPEQLNDTRPKDVIPGSNVVEGSLLPGMSSKS 1103

Query: 1081 SEDVNLVPHSSDNNT-NKVLEKASEDVPSLDATVTSLAS-DATIEPLPLKTAEVSVAIPP 1140
            +EDVNLVP SSD++T  K LEK SEDVP LDATVTS AS DAT+EPLPLK AE+SVAIPP
Sbjct: 1104 NEDVNLVPKSSDSHTIIKTLEKISEDVPRLDATVTSFASDDATVEPLPLKNAEISVAIPP 1163

Query: 1141 VEVRISEISIPESVPVLKVQEASMPVEKLGRDVVCKDETSLETELKNVEVQEPRKSSDKK 1200
              V   EIS+P SVP +KVQEA MP+EKL RD   +DETSLE E+KNVEVQEPRKSSDKK
Sbjct: 1164 AAVHNIEISVPNSVPAVKVQEAIMPMEKLARD-GSRDETSLEAEVKNVEVQEPRKSSDKK 1223

Query: 1201 TKKQKSSKLLSSDQAKDSKNSAIQQSKQSKSGKPELNDLKLKADSVVGKSSDTSSSPRKI 1260
            TKKQKSSKLLSSDQAKD KNS IQQSKQSKSGK E NDLKLK+D++VGKSSDT+ SPRKI
Sbjct: 1224 TKKQKSSKLLSSDQAKDFKNSGIQQSKQSKSGKSE-NDLKLKSDNIVGKSSDTACSPRKI 1283

Query: 1261 RDADAKIAVVDSQPFQRSASAVNSWNDAETVQVKDDSRLTGSDSVPNSQSQSGQRAWKTA 1320
            RD D KIA+VDSQ  Q SASAVNSWND ETV V+D+SRL+GSDSV NSQ+QSGQRAWK A
Sbjct: 1284 RDGDGKIAIVDSQLDQSSASAVNSWNDGETVPVRDESRLSGSDSVLNSQTQSGQRAWKIA 1343

Query: 1321 TSFRAKSLLEIQEEEQKMAHTEPAVSEISISVNSTSLSTPWAGIVSNLEPKASREIHKDL 1380
            ++F+ KSLLEIQEEEQKMAHTE  VS+IS S+N+ SLSTPWAGIVS+ +PKASREIHKD 
Sbjct: 1344 SNFKPKSLLEIQEEEQKMAHTETVVSDISTSINTMSLSTPWAGIVSSSDPKASREIHKDS 1403

Query: 1381 VNSEPSEKHESLLNSRSRKSQLHDLLAEDDMEKSGGVGDVRVSDSVQIASSPQVMTTQAE 1440
            VNSE SEKHE+LL SRSRKSQLHDLLAEDDMEKS G GDVRVSD+VQIASSPQVM  +AE
Sbjct: 1404 VNSESSEKHENLLTSRSRKSQLHDLLAEDDMEKS-GAGDVRVSDTVQIASSPQVMAVRAE 1463

Query: 1441 STDDNFIEAKETKKSRKKSAKAKGVGTKASASAPSADVPVASSPVEKGKISRQTQQEKDA 1500
              D+NFIEAK+TKKSRKKSAKAKG+GTKAS++ PSADVPV  SPVEKGKISRQ QQEK+A
Sbjct: 1464 PMDENFIEAKDTKKSRKKSAKAKGIGTKASSAVPSADVPVGLSPVEKGKISRQMQQEKEA 1523

Query: 1501 TPAIPSGPSFGDFVLWKGEAANVAPAPAWSSDSGKVHKPTSLRDIQKEQGRKLSSAQHSH 1560
             PAIPSGPSFGDFVLWKGE ANVAPA AWSSDSGKV KPTSLRDIQ EQ RK+S+AQH  
Sbjct: 1524 MPAIPSGPSFGDFVLWKGEVANVAPALAWSSDSGKVAKPTSLRDIQMEQERKISAAQHPQ 1583

Query: 1561 QIPTPQKAQPTQVGRSSSTSTPSWALSASSPSKAAS-PLQNIPSQSKYGGDDDLFWGPIE 1620
            QI TPQKAQPTQVGRSS T+TPSW+LSASSPSKAAS PLQNI +QSK+GGDDDLFWGPIE
Sbjct: 1584 QISTPQKAQPTQVGRSSRTTTPSWSLSASSPSKAASTPLQNIATQSKHGGDDDLFWGPIE 1643

Query: 1621 SKQENQQVDVRQGSHSSWGNRNTPSKAAAASTGLLSRQKSSGGKADYPSSSPAQSTQKGK 1680
            SKQENQ+VDVR GSH +WGNRNTP K A ASTGLLSRQKSSGGKAD+ SSSPAQS+QKGK
Sbjct: 1644 SKQENQRVDVRPGSHGNWGNRNTPVK-AVASTGLLSRQKSSGGKADHLSSSPAQSSQKGK 1703

Query: 1681 QDPITKHSEAMGFRDWCESECVRLIGTKDTSFLEFCLKQSRSEAELLLIENLGSFDPDHE 1740
            QDPITKHSEAMGFRDWCE+ECVRLIGTKDTSFLEFCLKQSRSEAELLLIENLGS+DPDHE
Sbjct: 1704 QDPITKHSEAMGFRDWCENECVRLIGTKDTSFLEFCLKQSRSEAELLLIENLGSYDPDHE 1763

Query: 1741 FIDQFLNYKELLPADVLDIAFQRRNDRKVSEIATRDMNSGSA-GGDLDPDVPV---GSAK 1800
            FIDQFLNYKELLPAD+++IAFQ RN+RKVS +A+RD+NSG+A GGDLDPDV     GSAK
Sbjct: 1764 FIDQFLNYKELLPADIIEIAFQSRNERKVSAMASRDVNSGNAGGGDLDPDVSAGRDGSAK 1823

Query: 1801 -GGGKKKGKKGKKVSPSVLGFNVVSNRIMMGEIQTVED 1822
             GGGKKKGKKGKK++PSVLGFNVVSNRIMMGEIQTVED
Sbjct: 1824 SGGGKKKGKKGKKINPSVLGFNVVSNRIMMGEIQTVED 1844

BLAST of MS016814 vs. TAIR 10
Match: AT5G42950.1 (GYF domain-containing protein )

HSP 1 Score: 1154.8 bits (2986), Expect = 0.0e+00
Identity = 822/1863 (44.12%), Postives = 1115/1863 (59.85%), Query Frame = 0

Query: 1    DVQGSENPIPLSPQWLLPKPGESKHGIGTGENHFSNPPAFRNRMDMMKASETYEDMNDMQ 60
            D+QGS+N IPLSPQWLL KPGE+K G+GTG+     P  + N  D+++ +   E+  D  
Sbjct: 28   DIQGSDNAIPLSPQWLLSKPGENKTGMGTGD-----PNQYGNHSDVVRTTGNGEETLDNL 87

Query: 61   KKKEVFRPSLADAEIGRRDRWHDEERENNSSMRKDRWRDGEKELGDSRKMDRWVEDSSTR 120
            KKK+VFRPSL DAE GRRDRW DEER+  SS+R DRWR+G+K+ GD++K+DRW  D+   
Sbjct: 88   KKKDVFRPSLLDAESGRRDRWRDEERDTLSSVRNDRWRNGDKDSGDNKKVDRW--DNVAP 147

Query: 121  VFRESRRGPSERWSDLSNRDNVHYDQRRESKWNTRWGPDDKETEGFREKRVDSGRDGDLH 180
             F E RRGP++RW+D  N+D    +QRRESKWN+RWGPDDKE E  R K  + G+DG++ 
Sbjct: 148  KFGEQRRGPNDRWTDSGNKDAAP-EQRRESKWNSRWGPDDKEAEIPRNKWDEPGKDGEII 207

Query: 181  PDKNLSHVSNYGKNDRDGDHYRPWRSSASQGRGKGEPPHHQTQTPVKQVPAFSH-RGRAD 240
             +K  S  ++      DGDHYRPWR   SQGRG+GE  H+Q+ TP KQV +FSH RGR +
Sbjct: 208  REKGPSLPTS------DGDHYRPWR--PSQGRGRGEALHNQS-TPNKQVTSFSHSRGRGE 267

Query: 241  NTPPTFSLGRGIISSGVSSTNNIYSSSNSLGASSEK--SG-RDPYHYKYSRTKLLDVYRT 300
            NT   FS GRG +S G S   +  + S+  G++S+K  SG  +P H +YSR KLLDVYR 
Sbjct: 268  NT-AIFSAGRGRMSPGGSIFTSAPNQSHPPGSASDKGESGPGEPPHLRYSRMKLLDVYRM 327

Query: 301  TNLTLEPSLKDGFVPVPSLTLEEPLEPLALCAPSTEEMTFLKGIDKGEIISSGAPQVSKD 360
             +        DGF+ VPSLT EEP +PLALCAPS++E+  L  I+KG+I+SSGAPQ SKD
Sbjct: 328  ADTECYEKFPDGFIEVPSLTSEEPTDPLALCAPSSDEVNVLDAIEKGKIVSSGAPQTSKD 387

Query: 361  ---GRNSSEFMQTRRAKLGVSPSPAGSREDLPHGFDDYNDDKDDSTTKPGHINYSELSTE 420
               GRN  EF Q RR +      PAGSRED+  G ++  D+  ++   P           
Sbjct: 388  GPTGRNPVEFSQPRRIR------PAGSREDMTFGAEESKDESGETRNYP----------- 447

Query: 421  RQMPPYHRPQSKIETIQEHMAHASGKFKSEAFREDDNAIRKADEVPVN--RESSVKGGAT 480
                                     KF+ EA  E     R+ +E PV   +E S++G A 
Sbjct: 448  -----------------------DDKFRPEASHEGYAPFRRGNEAPVRELKEPSMQGNAH 507

Query: 481  VHPGSAWDASSLEQPLNTSVPDWRDNHNNIGSGTPDKGWLQPPKNLNDGWGSNTATPSYP 540
            V   S W  SS  +  N +  DW D   +    + D  W  P  ++N   G+N   P   
Sbjct: 508  VQSASPWRQSSGGERSNRNSHDWNDPSADSRLKSSDSVWSHPKDSINHLGGNNMMLPQ-S 567

Query: 541  KDNPKWQTGEESIIRRQLSGILDKEQLARKTIQSAPEDMQLHYIDPSGAIQGPFSGADII 600
            K   +WQ  E+  +RRQ S + D+EQ  RK + S+PE++ L+Y DP G IQGPFSG+DII
Sbjct: 568  KGESRWQISEDPSLRRQPSLVFDREQEVRKLLPSSPEELSLYYKDPQGLIQGPFSGSDII 627

Query: 601  QWFEGGYFGLDLPVRPVSAPNDLPFSALGDVMPHLRSKAKPPPGFSGPKQNEFGDALGNT 660
             WFE GYFG+DL VR  SAPND PFS LGDVMPHLR+K+ PPPGF+G KQNEF DA G +
Sbjct: 628  GWFEAGYFGIDLLVRLASAPNDSPFSLLGDVMPHLRAKSGPPPGFTGAKQNEFVDAAGTS 687

Query: 661  SYGSLGKLHTGLNEIDTLRNETRHKHGSTVEAENRFLESLMSGNLGSSPLEKSAFSEGYF 720
            ++  +GK+H+G+ E D L+N+ R+KH +   AENRF+ESLMSG L +S    +   +GY 
Sbjct: 688  AFPGVGKVHSGMGETDMLQNDMRYKHVAGTVAENRFIESLMSGGLTNS----AQGVQGYG 747

Query: 721  GNNPNNLPSLGIDNGNNLFLLAKRMELERQRSLNNPYAFWPGIDATAKVSKPDVGLDDPI 780
             N+   L     D G +++LLAK++ELERQRS+ +PY++WPG ++   +   +       
Sbjct: 748  VNSSGGLSLPVTDGGADMYLLAKKLELERQRSIPSPYSYWPGRESANLMPGSE------- 807

Query: 781  QQAKLLSSIMDHSRQTSHSQSADMSAILQGLSDKAPPGIN-DVAAWSKFASQCASDPLQS 840
                   ++ ++++Q + S S+D+ +ILQG++D++ P ++  + AWS+        P+Q 
Sbjct: 808  -------NVSENAQQPTRSPSSDLLSILQGVTDRSSPAVSGPLPAWSQ--------PIQK 867

Query: 841  KLDLHHDLNLSSQAPFGFQQQRLQPQP-SLTNLLAQATDN---PTLTPDKFLSSSLSQDP 900
            + DLHH     +Q PFG QQQRL  Q   L+ LL Q  +N     L+PD  L++ LSQ+ 
Sbjct: 868  ESDLHHAKTFQTQIPFGVQQQRLPEQNLPLSGLLGQPMENNPGGMLSPDMMLAAGLSQEH 927

Query: 901  QLISKLQQQH-LLQLHSQVPFSAQ-QMSLLDKLLLLKQQQKQEEQQQLL-QQQQLLSQVL 960
            Q ++ LQQQ  LLQL++Q P SAQ Q  L++K+LLLK Q KQEEQQQLL QQQQL SQV 
Sbjct: 928  QSLNLLQQQQLLLQLNAQTPLSAQHQRLLVEKMLLLKHQHKQEEQQQLLRQQQQLYSQVF 987

Query: 961  SEHQ-SRQHFGDPSFGQLQGAPIPIGNASIDPSQVQLSREKFQIGSQKPLNVATTFGNMP 1020
            ++ Q S+Q FGDPS+GQLQ        AS+D  ++Q S++  Q+  Q  + V+     + 
Sbjct: 988  ADQQRSQQRFGDPSYGQLQ--------ASLDALRLQPSKDMSQVNQQVQVPVSHEERGIN 1047

Query: 1021 ----LQVTQGASYNV-NPEDPSLALPHQMFGNVIQQKSWNAALPEQFNDTHPKDMLPGSK 1080
                L VT   +  V + E PSL L +Q+FGNV  +      LP+Q +DTH K+      
Sbjct: 1048 LADLLPVTHATNQTVASFETPSLHLQNQLFGNVDPR----MVLPDQIDDTHKKE------ 1107

Query: 1081 VGEGPTLPGMISKSSEDVNLVPHSSDNNTNKVLEKASEDVPSLDATVTSLASDATIEPLP 1140
                       SKS  +  +    S +  N +  +     P   AT              
Sbjct: 1108 -----------SKSEYERTV----SADYVNSLYSEKPVLSPGYHAT-------------- 1167

Query: 1141 LKTAEVSVAIPPVEVRISEISIPESV--PVLKVQEASMPVEKLGRDVVCKDETSLETELK 1200
                E  V+ P  E   + ++ PE V   +L+ Q   M   K    +    ET   TE+K
Sbjct: 1168 -HNVEEPVSYPNNESSTATMTAPEIVESKLLEEQSKDMYAGKGEVSIELSGETP-ATEVK 1227

Query: 1201 NVEVQEPRKSSDKKTKKQKSSKLLSSDQAKDSKNSAIQQSKQSKSGKPELNDLKLKADSV 1260
            N +V   RK+S+KK++KQ++ +  ++D AK +  + +Q++K+ + G  + +++K K    
Sbjct: 1228 NNDVSVARKTSEKKSRKQRAKQ--AADLAKSTSRAPLQETKKPQPGSADDSEIKGKTK-- 1287

Query: 1261 VGKSSDTSSSPRKIRDADAKIAVVDSQPFQRSASAVNSWNDAETVQVKDDSRLTGSDSVP 1320
              KS+DT               ++D+      +S   + N ++     D  R   S S+ 
Sbjct: 1288 --KSADT---------------LIDNDTHLIKSSTATASNTSQMSSEVDSVRGEES-SLQ 1347

Query: 1321 NSQSQSGQRAWKTATSFRAKSLLEIQEEEQKMAHTEPAVSEISISVNSTSLSTPWAGIVS 1380
            N+++Q G RAWK A  F+ KSLLEIQ EEQ++A  E    +IS +VNS   + PWAGIV+
Sbjct: 1348 NTRTQPG-RAWKPAPGFKPKSLLEIQMEEQRVAQAEALAPKISSTVNSVGSAAPWAGIVT 1407

Query: 1381 NLEPKASREIH-KDLVNSEPSEKHESLLNSRSRKSQLHDLLAEDDMEKSGGVGDVRVSDS 1440
            N +    RE H +  +      K ES+   +++KS LHDLLA+D   KS      +  + 
Sbjct: 1408 NSDSNILRETHGESAITQTGVVKPESVPTLKAKKSHLHDLLADDVFAKSSD----KEREV 1467

Query: 1441 VQIASS----PQVMTTQAES-TDDNFIEAKETKKSRKKSAKAKGVGTKASASAPSADVPV 1500
            ++I S+     QV TT AES  DDNFI+A+ETKKSRKKSA+AK  G K +A  P+ D  +
Sbjct: 1468 MEIISNNDAFMQVTTTNAESFDDDNFIDARETKKSRKKSARAKTSGAKIAAHVPAVDTSL 1527

Query: 1501 ASSPVEKGKISR-QTQQEKDATPAIPSGPSFGDFVLWKGEAA-NVAPAPAWSSDSGKVHK 1560
             ++ VEKGK SR   QQEK+  PAIPSGPS GDFVLWKGE+  N  PA AWSS   K  K
Sbjct: 1528 QTNSVEKGKSSRILQQQEKEVLPAIPSGPSLGDFVLWKGESVNNPPPAAAWSSGPKKSTK 1587

Query: 1561 PTSLRDIQKEQGRKLSSAQH--SHQIPTPQKAQPTQVGRSSSTSTPSWALSASSPSKAAS 1620
            P+SLRDI KEQ  K++++ H     +PT QKA P Q  +  +    SW+ SASSPS+A S
Sbjct: 1588 PSSLRDIVKEQ-EKMTTSSHPPPSPVPTTQKAIPPQAHQGGA----SWSRSASSPSQAVS 1647

Query: 1621 PLQNIPSQSKYGGDDDLFWGPIE-SKQENQQVDV-RQGSHSSWGNRNTPSKAAAASTGLL 1680
                  SQSK  GDDDLFWGP+E S Q+ +Q D     S +SWG +NTP K  A ++  L
Sbjct: 1648 ---QSSSQSKSKGDDDLFWGPVEQSTQDTKQGDFPHLTSQNSWGTKNTPGKVNAGTS--L 1707

Query: 1681 SRQKS-SGGKADYPSSSP--AQSTQKGKQDPITKHSEAMGFRDWCESECVRLIGTKDTSF 1740
            +RQKS S G AD   SSP   Q++ KGK++ +TK +EA GFRDWC+SEC+RL+G++DTS 
Sbjct: 1708 NRQKSVSMGSADRVLSSPVVTQASHKGKKEAVTKLTEANGFRDWCKSECLRLLGSEDTSV 1714

Query: 1741 LEFCLKQSRSEAELLLIENLGSFDPDHEFIDQFLNYKELLPADVLDIAFQRRNDRKVSEI 1800
            LEFCLK SRSEAE LLIENLGS DPDH+FID+FLNYK+LLP++V++IAFQ     K S +
Sbjct: 1768 LEFCLKLSRSEAETLLIENLGSRDPDHKFIDKFLNYKDLLPSEVVEIAFQ----SKGSGV 1714

Query: 1801 ATRDMNSGSAGGDLDPDVPVGSAKGGGKKKGKKGKKV--SPSVLGFNVVSNRIMMGEIQT 1822
             TR+ N+G            G +K GGKKK KKGKKV  S SVLGFNVVSNRIMMGEIQT
Sbjct: 1828 GTRN-NTGEDYYYNTTAANDGFSKVGGKKKAKKGKKVSLSASVLGFNVVSNRIMMGEIQT 1714

BLAST of MS016814 vs. TAIR 10
Match: AT1G24300.2 (GYF domain-containing protein )

HSP 1 Score: 156.0 bits (393), Expect = 2.9e-37
Identity = 192/643 (29.86%), Postives = 280/643 (43.55%), Query Frame = 0

Query: 5   SENPIPLSPQWLLPKPGESKHGIGTGENHFSNPPAFRNRMDMMKASETYEDMNDMQKKKE 64
           S+N IPLSPQWL  K  ESK  + +        P+  N  D  +         D  + K+
Sbjct: 26  SDNSIPLSPQWLYTKSSESKMDVRSPTPMPMGNPSDPNLKDAWRL--------DAPEDKK 85

Query: 65  VFRPSLADAEIGRRDRWHDEERENNSSMRKDRWRDGEKELGDSRKMDRWVEDSSTRVFRE 124
            ++  +++ E  R  RW +EERE            G +++ D RK +R +++ S+R   E
Sbjct: 86  DWKKIVSENETNR--RWREEERETGLL--------GARKV-DRRKTERRIDNVSSRETGE 145

Query: 125 SR-RGPSERWSDLSNRDNVHYDQRRESKWNTRWGPDDKETEGFREKRVDSGRDGDLHPDK 184
            +    S+RW+D+++R  VH + RR++KW++RWGPDDKE E  R ++V+  +D +    +
Sbjct: 146 VKTTAASDRWNDVNSRAAVH-EPRRDNKWSSRWGPDDKEKEA-RCEKVEINKDKEEPQSE 205

Query: 185 NLSHVSNY-GKNDRDGDHYRPWRSSASQGRGKGEPPHHQTQTPVKQVPAFS-HRGRADNT 244
           + S VSN    ++RD D    WR         G P  ++T       P F   RGRA+  
Sbjct: 206 SQSVVSNVRATSERDSDPRDKWRPRHRMESQSGVPTSYRT------APGFGLDRGRAEGP 265

Query: 245 PPTFSLGRGIISSGVSSTNNIYSSSNSLGASSEKSGRDPYHYKYSRTKLLDVYRTTNLTL 304
              F++GR     G +ST    SS++ +GA S  +      ++Y R KLLD+YR      
Sbjct: 266 NLGFTVGR-----GRASTIGRGSSTSLIGAGSASAP----VFRYPRGKLLDMYRKQK--P 325

Query: 305 EPSLKDGFVP-----VPSLTLEEPLEPLALCAPSTEEMTFLKGIDKGEIISSGAPQVSKD 364
           +PSL  G +P     V S+T    +EPLA  AP TEE   + GI KG IISS        
Sbjct: 326 DPSL--GRIPTEMDEVASITQVALIEPLAFIAPDTEEEASINGIWKGRIISS-------- 385

Query: 365 GRNSSEFMQTRRAKLGVSPSPAGSREDLPHGFDDYNDDKDDSTTKPGHINYSELSTERQM 424
                                                   +  T  G  +  E S  +  
Sbjct: 386 ----------------------------------------EVYTSSGEESLGENSLLKCR 445

Query: 425 PPYHRPQSKIETIQEHMAHASGKFKSE----AFREDDNAIRKADEVPVNRESSVKGGATV 484
            P                  SG+ K +     F   DN   K      N +S + G    
Sbjct: 446 IP-----------------ESGETKVDGALLGFMNGDNGSMK------NNDSGLLGS--- 505

Query: 485 HPGSAWDASSLEQPLNTSVPDWRDNHNNIGSGTPDKGWLQPPKNLNDGWGSNTATPSYPK 544
           H G    ASS+  P   SV    +++ + G+G                 GS  A  S   
Sbjct: 506 HNGGLGAASSV--PRLNSVAS--ESYGSFGAG------------YQVSHGSPEAVRSVFT 537

Query: 545 DNPKWQTGEESII---RRQLSGILDKEQLARKTIQSA--PEDMQLHYIDPSGAIQGPFSG 604
            +P    G ES++    +   G L +  +     ++A  PED    YIDP G IQGPF G
Sbjct: 566 KSPVLD-GSESVVGSFEQDYMGKLQQPDVEVDQSEAAMPPEDFLFLYIDPQGVIQGPFIG 537

Query: 605 ADIIQWFEGGYFGLDLPVRPVSAPNDLPFSALGDVMPHLRSKA 631
           +DII WFE G+FG DL VR  +AP   PF  LG VM +L++++
Sbjct: 626 SDIISWFEQGFFGTDLQVRLANAPEGTPFQDLGRVMSYLKTES 537

HSP 2 Score: 53.9 bits (128), Expect = 1.6e-06
Identity = 28/45 (62.22%), Postives = 34/45 (75.56%), Query Frame = 0

Query: 1777 DVPVGSAKGGGKKKGKKGKKVSPSVLGFNVVSNRIMMGEIQTVED 1822
            DV  GS  GGGKKKGKKG+++ P++LGF V SNRI MGEI   +D
Sbjct: 1446 DVTEGSKGGGGKKKGKKGRQIDPALLGFKVTSNRI-MGEIHRADD 1489

BLAST of MS016814 vs. TAIR 10
Match: AT1G24300.1 (GYF domain-containing protein )

HSP 1 Score: 138.7 bits (348), Expect = 4.8e-32
Identity = 256/1001 (25.57%), Postives = 392/1001 (39.16%), Query Frame = 0

Query: 5   SENPIPLSPQWLLPKPGESKHGIGTGENHFSNPPAFRNRMDMMKASETYEDMNDMQKKKE 64
           S+N IPLSPQWL  K  ESK  + +        P+  N  D  +         D  + K+
Sbjct: 26  SDNSIPLSPQWLYTKSSESKMDVRSPTPMPMGNPSDPNLKDAWRL--------DAPEDKK 85

Query: 65  VFRPSLADAEIGRRDRWHDEERENNSSMRKDRWRDGEKELGDSRKMDRWVEDSSTRVFRE 124
            ++  +++ E  R  RW +EERE            G +++ D RK +R +++ S+R   E
Sbjct: 86  DWKKIVSENETNR--RWREEERETGLL--------GARKV-DRRKTERRIDNVSSRETGE 145

Query: 125 SR-RGPSERWSDLSNRDNVHYDQRRESKWNTRWGPDDKETEGFREKRVDSGRDGDLHPDK 184
            +    S+RW+D+++R  VH + RR++KW++RWGPDDKE E  R ++V+  +D +    +
Sbjct: 146 VKTTAASDRWNDVNSRAAVH-EPRRDNKWSSRWGPDDKEKEA-RCEKVEINKDKEEPQSE 205

Query: 185 NLSHVSNY-GKNDRDGDHYRPWRSSASQGRGKGEPPHHQTQTPVKQVPAFS-HRGRADNT 244
           + S VSN    ++RD D    WR         G P  ++T       P F   RGRA+  
Sbjct: 206 SQSVVSNVRATSERDSDPRDKWRPRHRMESQSGVPTSYRT------APGFGLDRGRAEGP 265

Query: 245 PPTFSLGRGIISSGVSSTNNIYSSSNSLGASSEKSGRDPYHYKYSRTKLLDVYRTTNLTL 304
              F++GR     G +ST    SS++ +GA S  +      ++Y R KLLD+YR      
Sbjct: 266 NLGFTVGR-----GRASTIGRGSSTSLIGAGSASAP----VFRYPRGKLLDMYRKQK--P 325

Query: 305 EPSLKDGFVP-----VPSLTLEEPLEPLALCAPSTEEMTFLKGIDKGEIISSGAPQVSKD 364
           +PSL  G +P     V S+T    +EPLA  AP TEE   + GI KG IISS        
Sbjct: 326 DPSL--GRIPTEMDEVASITQVALIEPLAFIAPDTEEEASINGIWKGRIISS-------- 385

Query: 365 GRNSSEFMQTRRAKLGVSPSPAGSREDLPHGFDDYNDDKDDSTTKPGHINYSELSTERQM 424
                                                   +  T  G  +  E S  +  
Sbjct: 386 ----------------------------------------EVYTSSGEESLGENSLLKCR 445

Query: 425 PPYHRPQSKIETIQEHMAHASGKFKSE----AFREDDNAIRKADEVPVNRESSVKGGATV 484
            P                  SG+ K +     F   DN   K      N +S + G    
Sbjct: 446 IP-----------------ESGETKVDGALLGFMNGDNGSMK------NNDSGLLGS--- 505

Query: 485 HPGSAWDASSLEQPLNTSVPDWRDNHNNIGSGTPDKGWLQPPKNLNDGWGSNTATPSYPK 544
           H G    ASS+  P   SV    +++ + G+G                 GS  A  S   
Sbjct: 506 HNGGLGAASSV--PRLNSVAS--ESYGSFGAG------------YQVSHGSPEAVRSVFT 565

Query: 545 DNPKWQTGEESII---RRQLSGILDKEQLARKTIQSA--PEDMQLHYIDPSGAIQGPFSG 604
            +P    G ES++    +   G L +  +     ++A  PED    YIDP G IQGPF G
Sbjct: 566 KSPVLD-GSESVVGSFEQDYMGKLQQPDVEVDQSEAAMPPEDFLFLYIDPQGVIQGPFIG 625

Query: 605 ADIIQWFEGGYFGLDLPVRPVSAPNDLPFSALGDVMPHLRSKAKPPPGFSGPKQNEFGDA 664
           +DII WFE G+FG DL VR  +AP   PF  LG VM +L++++                 
Sbjct: 626 SDIISWFEQGFFGTDLQVRLANAPEGTPFQDLGRVMSYLKTES----------------- 685

Query: 665 LGNTSYGSLGKLHTGLNEIDTLRNETRHKHGSTV------EAENRFLESLMSGNLGSSPL 724
                       H  ++  ++   ETR K  S         AE+    S+   +   S  
Sbjct: 686 -----------AHAHISNQESELEETRLKANSDTGLSIAPVAESNDSSSMNGTSRSFSVY 745

Query: 725 EKSAFSEGYFGNNPNNLPSLGIDNGNNLFLLAKRMELERQRSLNNPYAFWPGIDATAKVS 784
              +  + +   + +   +      +  FL     + E        +    G+   A V 
Sbjct: 746 NNPSAQDNFQRKSESEFYATPPHTEDRSFLDFSTQDEE------IVFPGRAGVSGYASV- 805

Query: 785 KPDVGLDDPIQQAKLLSSIMDHS----RQTSHSQSADMSAILQGLSDKAPPGIN-----D 844
           K    + D   +    S+I   S     Q  H        +L    + +   +N      
Sbjct: 806 KSSTSMHDAFMEVSGQSAIPVESTKAATQKQHENKLHPFGVLWSELESSNVPVNLLPNRS 856

Query: 845 VAAWSKFASQCASDPLQSKLDLHHDLNLSSQAPFGFQQQRLQPQPSLTNLLAQATDN--- 904
             A  +      + P+ S+ +   D N+S       +  + +   +  NL  Q + N   
Sbjct: 866 YDAMGEPTGAIDNRPIDSRRNTQVDPNMSLDGLASNRMSQFEHLSNRFNLGDQLSSNQHN 856

Query: 905 -------PTLTPDKFLSSSLSQDPQLISKLQQQHLLQLHSQVPFSAQQMSLLDKLLLLKQ 963
                    L+       +   D  +  +LQQQ  +QL  Q     QQ   +     L+Q
Sbjct: 926 QQQFQNRDMLSHSHIGDQAQDLDYLITLQLQQQQKIQLQQQQKIQLQQQQKIQ----LQQ 856

HSP 2 Score: 53.9 bits (128), Expect = 1.6e-06
Identity = 28/45 (62.22%), Postives = 34/45 (75.56%), Query Frame = 0

Query: 1777 DVPVGSAKGGGKKKGKKGKKVSPSVLGFNVVSNRIMMGEIQTVED 1822
            DV  GS  GGGKKKGKKG+++ P++LGF V SNRI MGEI   +D
Sbjct: 1451 DVTEGSKGGGGKKKGKKGRQIDPALLGFKVTSNRI-MGEIHRADD 1494

BLAST of MS016814 vs. TAIR 10
Match: AT1G27430.1 (GYF domain-containing protein )

HSP 1 Score: 115.9 bits (289), Expect = 3.3e-25
Identity = 253/989 (25.58%), Postives = 376/989 (38.02%), Query Frame = 0

Query: 5   SENPIPLSPQWLLPKPGESKHGIGTGENHFSNPPAFRNRMDMMKASETYEDMNDMQKKKE 64
           S+N IPLSPQWL  K  E K  + +       P    N  D                 K+
Sbjct: 26  SDNSIPLSPQWLYTKSSEYKMDVRS-----PTPVPMGNPSD--------------PNPKD 85

Query: 65  VFRPSLADAEIGRRDRWHDEERENNSSMRKDRWRDGEKELG-------DSRKMDRWVEDS 124
            +R    DA   ++D W     EN +S    RWR+ E+E G       D RK +R ++  
Sbjct: 86  AWR---LDAPEDKKD-WKKIVHENETSR---RWREEERETGLLGARKVDRRKTERRIDSV 145

Query: 125 STRVFRESRR-GPSERWSDLSNRDNVHYDQRRESKWNTRWGPDDKETEGFREKRVDSGRD 184
           S+R   + +    S+RW+D+++R  VH + RR++KW++RWGPDDKE E  R ++VD  +D
Sbjct: 146 SSRETGDIKNAAASDRWNDVNSRAAVH-EPRRDNKWSSRWGPDDKEKEA-RCEKVDINKD 205

Query: 185 GDLHPDKNLSHVSNY-GKNDRDGDHYRPWRSSASQGRGKGEPPHHQTQTPVKQVPAFS-H 244
            +    ++ S VSN    ++RD D    WR         G P  +      +  P F   
Sbjct: 206 KEEPQSESQSVVSNVRATSERDSDTRDKWRPRHRMESQSGGPSSY------RAAPGFGLD 265

Query: 245 RGRADNTPPTFSLGRGIISSGVSSTNNIYSSSNSLGASSEKSGRDPYHYKYSRTKLLDVY 304
           RGRA+     F++GR     G +ST    SS++ +GA S  S      ++Y R KLLD+Y
Sbjct: 266 RGRAEGPNLGFTVGR-----GRASTIGRGSSTSLIGAGSALSP----VFRYPRGKLLDMY 325

Query: 305 RTTNLTLEPSLKDGFV-----PVPSLTLEEPLEPLALCAPSTEEMTFLKGIDKGEIISSG 364
           R      +P    G +      V S+T    +EPLA  AP  EE   L GI KG IISS 
Sbjct: 326 RKQ----KPDSSLGRILTEMDEVASITQVALIEPLAFIAPDAEEEANLNGIWKGRIISSE 385

Query: 365 APQVS-KDGRNSSEFMQTRRAKLGVSPSPAGSREDLPHGFDDYNDDKDDSTTKPGHINYS 424
               S ++    +  ++ R  + G      G+     +G D+ +   +DS     H    
Sbjct: 386 VYTSSGEESLGGNSLLKCRIPESG-ETKVDGALLGFMNG-DNGSMKNNDSGLLGSH--NG 445

Query: 425 ELSTERQMPPYHRPQSKIETIQEHMAHASGKFKSEAFREDDNAIRKADEVPVNRESSVKG 484
            L     +P  +   S+        ++ SG    +       A+R           SV  
Sbjct: 446 GLGAASSVPRLNSVASE--------SYGSGGAGYQLSHGSPEAVR-----------SVFT 505

Query: 485 GATVHPGSAWDASSLEQPLNTSVPDWRDNHNNIGSGTPDKGWLQPPKNLNDGWGSNTATP 544
            ++V  GS     S EQ                       G LQ P    D   S  A P
Sbjct: 506 KSSVLDGSESVVGSFEQAYT--------------------GKLQQPDTEVD--HSEGAMP 565

Query: 545 SYPKDNPKWQTGEESIIRRQLSGILDKEQLARKTIQSAPEDMQLHYIDPSGAIQGPFSGA 604
                                                 PE+    YIDP G IQGPF G+
Sbjct: 566 --------------------------------------PEEFLFLYIDPQGVIQGPFIGS 625

Query: 605 DIIQWFEGGYFGLDLPVRPVSAPNDLPFSALGDVMPHLRSKAKPPPGFSGPKQNEFGDAL 664
           DII WFE G+FG DL VR  SAP   PF  LG VM ++++++                  
Sbjct: 626 DIISWFEQGFFGTDLQVRLASAPEGTPFQDLGRVMSYIKAES------------------ 685

Query: 665 GNTSYGSLGKLHTGLNEIDTLRNETRHKHGSTVEAENRFLESLMSGNLGSSPLEKSAFSE 724
                     +H  +++  +   ET  K  S  EA         S +  S      +FS 
Sbjct: 686 ----------VHAHISDQKSELEETSLKANS--EAGGSVAHVAESNDSSSLTGISRSFS- 745

Query: 725 GYFGNNPNNLPSLGIDNGNNLFLLAKRMELER--QRSLNNPYAFWPG---IDATAKVSKP 784
               NNP+   +    + + ++      E +     S  +    +PG   +   A   K 
Sbjct: 746 --VYNNPSGQDNFQRKSESEVYGRPPHAEDQSFLDFSAQDEEIVFPGRARVSGYASSVKS 805

Query: 785 DVGLDDPIQQAKLLSSI---------MDHSRQTSHSQSADMSAILQGLSDKAPPGINDVA 844
              + D + +    S I          + +    H      S +  G +   P       
Sbjct: 806 STSMHDALMEFSGHSDIPVEVTTAATRNQNENKLHPFGVLWSELEGGSTPVNPLPNRSSG 847

Query: 845 AWSKFASQCASDPLQSKLDLHHDLNLSSQAPFGFQQQRLQPQPSLTNLLAQATDNPTLTP 904
           A  + +    + P+ S+ +   D N+S  A  G +  + + + +  N   Q   N     
Sbjct: 866 AMGEPSCSIENRPINSRRNSQIDPNISLDALSGNRMSQFEHESNFFNHGDQLPSNQHHQQ 847

Query: 905 DKFLSSSLSQDPQLISKLQQQHLLQLHSQVPFSAQQMSLLDKLLLLKQQQKQEEQQQLLQ 963
                  LS        L+    LQL  Q     QQ   +     L+QQQK + QQ  L+
Sbjct: 926 HFQNRDMLSHLHIGDQDLEHLITLQLQQQQKIQMQQQQKIQ----LQQQQKIQLQQHQLE 847

HSP 2 Score: 57.8 bits (138), Expect = 1.1e-07
Identity = 27/45 (60.00%), Postives = 34/45 (75.56%), Query Frame = 0

Query: 1777 DVPVGSAKGGGKKKGKKGKKVSPSVLGFNVVSNRIMMGEIQTVED 1822
            D   GS  GGGKKKGKKG+++ P++LGF V SNRI+MGEI   +D
Sbjct: 1447 DATEGSKGGGGKKKGKKGRQIDPALLGFKVTSNRILMGEIHRADD 1491