MS016812 (gene) Bitter gourd (TR) v1

BLAST of MS016812 vs. NCBI nr
Match: XP_022144353.1 (uncharacterized protein LOC111014058 [Momordica charantia])

HSP 1 Score: 1513.8 bits (3918), Expect = 0.0e+00
Identity = 782/819 (95.48%), Postives = 786/819 (95.97%), Query Frame = 0

Query: 1   MELRSYNHFHYIHAIKGGLMTKVLNINSRGKPAVVFKKLTDIYEFIDEKDQNSLPTQLLR 60
           MELRSYNHFHYIHAIKGGLMTKVLNINSRGKPAVVFKKLTD+YEFIDEKDQNSLPTQLLR
Sbjct: 1   MELRSYNHFHYIHAIKGGLMTKVLNINSRGKPAVVFKKLTDVYEFIDEKDQNSLPTQLLR 60

Query: 61  ERLEENIPEGYKFKVETEDFYAERKLFKDEPTVSDSGSGGDTDGQKSDVEVDSMTIQQIM 120
           ERLEENIPEGYKFKVETEDFYAERKLFKDEPTVSDSGSGGDTDGQKSDVEVDSMTIQQIM
Sbjct: 61  ERLEENIPEGYKFKVETEDFYAERKLFKDEPTVSDSGSGGDTDGQKSDVEVDSMTIQQIM 120

Query: 121 EGCKKRKSRQSKSVDSSKEKLRTCSKQELERSCLLSDEDDDSDLNVALSIWKSKLSRRKK 180
           EGCKKRKSRQSKSVDSSKEKLRTCSKQELERSCLLSDEDDDSDLNVALS+WKSKLSRRKK
Sbjct: 121 EGCKKRKSRQSKSVDSSKEKLRTCSKQELERSCLLSDEDDDSDLNVALSVWKSKLSRRKK 180

Query: 181 LKTKCNGSRISTSSQCSQITGNSDPINSDQDLLPSSADLPIPVDIKVETPETDVTEIQNT 240
           LKTKCNGSRISTSSQCSQITGNSDPINSDQDLLPSSADLPIPVD+KVETPETDVTEIQNT
Sbjct: 181 LKTKCNGSRISTSSQCSQITGNSDPINSDQDLLPSSADLPIPVDVKVETPETDVTEIQNT 240

Query: 241 NYIIDDLSLLCDENVNSCLSSELSLLCDENVNLCLSSEPIGADELFLNRGSTTSNKEAEY 300
           NYIIDDLSLLCDENVNSCLSSELSLLCDENVNLCLSSEPIGADELFLNRGSTTSNKEAEY
Sbjct: 241 NYIIDDLSLLCDENVNSCLSSELSLLCDENVNLCLSSEPIGADELFLNRGSTTSNKEAEY 300

Query: 301 CVLNSACHEYLVGDDPEFLQMVGESNTEWMKKDNLEIQKPNYSDFPASESMEGRYAPRCL 360
           CVLNSACHEYLVGDDPEFLQMVGESNTEWMKKDNLEIQKPNYSDFPASESMEGRYAPRCL
Sbjct: 301 CVLNSACHEYLVGDDPEFLQMVGESNTEWMKKDNLEIQKPNYSDFPASESMEGRYAPRCL 360

Query: 361 SNDSMSEAISLTEEQCSGTYISQGKSITHEAICQNNCEDMSEEISPTEEQCTDTYISEGK 420
           SNDSMSE ISLTEEQCSGTYISQGKSITHEAICQNNCEDMSEEISPTEEQCTDTYISE  
Sbjct: 361 SNDSMSEEISLTEEQCSGTYISQGKSITHEAICQNNCEDMSEEISPTEEQCTDTYISE-- 420

Query: 421 SLTHETICLNNGEVLNHLHGMTNLNSLQLPEMSFGAEVCLTENGYKDTLELDHDRKGIST 480
                                         EMSFGAEVCLTENGYKDTLELDH+RKGIST
Sbjct: 421 ------------------------------EMSFGAEVCLTENGYKDTLELDHERKGIST 480

Query: 481 EATSDCDLRADHGESISTKSTTDCNLSPDHEKSISTSSTSDGNLSPDQHLISIGKCPAQE 540
           EATSDCDLRADHGESISTKSTTDCNLSPDHEKSISTSSTSDGNLSPDQHLISIGKCPAQE
Sbjct: 481 EATSDCDLRADHGESISTKSTTDCNLSPDHEKSISTSSTSDGNLSPDQHLISIGKCPAQE 540

Query: 541 IEPQISNFSDSERNTSPDFHLDDSMDKFNQFEEPKRHPTRLLSTRTTISPTSQERLSKAM 600
           IEPQISNFSDSERNTSPDFHLDDSMDKFNQFEEPKRHPTRLLSTRTTISPTSQERLSKAM
Sbjct: 541 IEPQISNFSDSERNTSPDFHLDDSMDKFNQFEEPKRHPTRLLSTRTTISPTSQERLSKAM 600

Query: 601 KSMRLHDKECKTCGGKPYFKQANYKVGTAEECDQMKRVYSDIFHEQNIRKSKKRSLHSTS 660
           KSMRLHDKECKTCGGKPYFKQANYKVGTAEECDQMKRVYSDIFHEQNIRKSKKRSLHSTS
Sbjct: 601 KSMRLHDKECKTCGGKPYFKQANYKVGTAEECDQMKRVYSDIFHEQNIRKSKKRSLHSTS 660

Query: 661 NTKVPHGRTRSTAVQSCSDNAIAFTQRQMQDIESIALKLTNQLKSMKAIVEDRLHVEGNK 720
           NTKVPHGRTRSTAVQSCSDNAIAFTQRQMQDIESIALKLTNQLKSMKAIVEDRLHVEGNK
Sbjct: 661 NTKVPHGRTRSTAVQSCSDNAIAFTQRQMQDIESIALKLTNQLKSMKAIVEDRLHVEGNK 720

Query: 721 ATGFKFNTDEVRTAISDATKAEASAKKWLSMMSRDCNRFCKIMKTTENGSTASPSAIQKI 780
           ATGFKFNTDEVRTAISDATKAEASAKKWLSMMSRDCNRFCKIMKTTENGSTASPSAIQKI
Sbjct: 721 ATGFKFNTDEVRTAISDATKAEASAKKWLSMMSRDCNRFCKIMKTTENGSTASPSAIQKI 780

Query: 781 KRKITFADEAGGKLCEVRLIEDHVESFVEASPEKYEAVK 820
           KRKITFADEAGGKLCEVRLIEDHVESFVEASPEKYEAVK
Sbjct: 781 KRKITFADEAGGKLCEVRLIEDHVESFVEASPEKYEAVK 787

BLAST of MS016812 vs. NCBI nr
Match: XP_023536738.1 (dentin sialophosphoprotein-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1080.1 bits (2792), Expect = 0.0e+00
Identity = 589/820 (71.83%), Postives = 669/820 (81.59%), Query Frame = 0

Query: 1   MELRSYNHFHYIHAIKGGLMTKVLNINSRGKPAVVFKKLTDIYEFIDEKDQNSLPTQLLR 60
           MELRS++HFH+IHAIKGG+MTKVLNIN RGKPA+VFKKLTDIYE I +K Q SLPT+  R
Sbjct: 1   MELRSFSHFHHIHAIKGGMMTKVLNIN-RGKPAMVFKKLTDIYESIYDKAQESLPTRWSR 60

Query: 61  ERLEENIPEGYKFKVETEDFYAERKLFKDEPTVSDSGSGG--DTDGQKSDVEVDSMTIQQ 120
           E LE NI +G + K+ET+  YAERKLF DEP VSDS   G  DT+GQKSDVE DSMTI+Q
Sbjct: 61  EGLEGNIRDGCESKMETQVLYAERKLFNDEPEVSDSDREGDSDTEGQKSDVEADSMTIKQ 120

Query: 121 IMEGCKKRKSRQSKSVDSSKEKLRTCSKQELERSCLLSDEDDDSDLNVALSIWKSKLSRR 180
           +ME CKKRK RQS SVDSSKEKLRTCS +EL  SCLLSDE DDSDL+VALSIW+SKLS+R
Sbjct: 121 MMESCKKRKVRQSNSVDSSKEKLRTCSSRELNHSCLLSDE-DDSDLDVALSIWQSKLSKR 180

Query: 181 KKLKTKCNGSRISTSSQCSQITGNSDPINSDQDLLPSSADLPIPVDIKVETPETDVTEIQ 240
           KKLKTKC+ S+ISTSS   Q   NSDPIN DQDLLPSS+DL IPVDIKVETPETDVTEIQ
Sbjct: 181 KKLKTKCDESKISTSSLHGQTIENSDPINIDQDLLPSSSDLAIPVDIKVETPETDVTEIQ 240

Query: 241 NTNYIIDDLSLLCDENVNSCLSSELSLLCDENVNLCLSSEPIGADELFLNRGSTTSNKEA 300
           NTN                C++ ELSLLCDENVN CLSS PIG DELF     T S KEA
Sbjct: 241 NTN----------------CITDELSLLCDENVNSCLSSGPIGTDELFFGLELTASEKEA 300

Query: 301 EYCVLNSACHEYLVGDDPEFLQMVGESNTEWMKKDNLEIQKPNYSDFPASESMEGRYAPR 360
           EY V N    E + GD+   LQMVGES+TE + +DNLE+ KP +SDFPASE+MEG+  P 
Sbjct: 301 EYGVPNRVSLENVEGDESRPLQMVGESSTECVSEDNLEVHKPQHSDFPASETMEGQCTPS 360

Query: 361 CLSNDSMSEAISLTEEQCSGTYISQGKSITHEAICQNNCEDMSEEISPTEEQCTDTYISE 420
            +SNDS+SEAISLTEEQC G ++SQ KSITHE ICQNN EDMS  IS T EQ +DT+ISE
Sbjct: 361 FVSNDSISEAISLTEEQCLGIHMSQAKSITHEGICQNNSEDMS-AISMTGEQFSDTHISE 420

Query: 421 GKSLTHETICLNNGEVLNHLHGMTNLNSLQLPEMSFGAEVCLTENGYKDTLELDHDRKGI 480
           GK  T E IC  NGE+  +L+GM +LNSLQLPEMS GAEV LTEN YKD L  D++ KGI
Sbjct: 421 GKPFTDEAICPTNGEIFTYLNGMADLNSLQLPEMSLGAEVRLTENRYKDRLAFDNE-KGI 480

Query: 481 STEATSDCDLRADHGESISTKSTTDCNLSPDHEKSISTSSTSDGNLSPDQHLISIGKCPA 540
            TE+TSDC+L ++HG  IS+KST+DCNLSPDH +S+ST+S SD NL PDQHLISI +CPA
Sbjct: 481 PTESTSDCNLSSEHGGRISSKSTSDCNLSPDHGESVSTNSISDRNLIPDQHLISIDECPA 540

Query: 541 QEIEPQISNFSDSERNTSPDFHLDDSMDKFNQFEEPKRHPTRLLSTRTTISPTSQERLSK 600
           +E +PQ+S+ SDSERNTSPDFHL+ S DKFNQ EEP+RHPTRLLSTRTTISPTSQERLSK
Sbjct: 541 KEKQPQMSDSSDSERNTSPDFHLNGSTDKFNQIEEPQRHPTRLLSTRTTISPTSQERLSK 600

Query: 601 AMKSMRLHDKECKTCGGKPYFKQANYKVGTAEECDQMKRVYSDIFHEQNIRKSKKRSLHS 660
           AMKSM+L DKECKTCGGKPYFKQ  YKVGTAE CD MKRVYSD +HEQN RKS+KRSLHS
Sbjct: 601 AMKSMQLQDKECKTCGGKPYFKQIKYKVGTAEGCDPMKRVYSDTYHEQNTRKSRKRSLHS 660

Query: 661 TSNTKVPHGRT--RSTAVQSCSDNAIAFTQRQMQDIESIALKLTNQLKSMKAIVEDRLHV 720
           T+ TK  H     RS+ VQSCSD+AIAFT+RQMQDIE +ALKLT QLKSMKAIVEDR+HV
Sbjct: 661 TNTTKASHAHASMRSSTVQSCSDSAIAFTERQMQDIECLALKLTTQLKSMKAIVEDRIHV 720

Query: 721 EGNKATGFKFNTDEVRTAISDATKAEASAKKWLSMMSRDCNRFCKIMKTTENGSTASPSA 780
           EGNKAT +KFNTDEVRTAI+DATKAEASAKKWLS+MSRDCNRFCKIMKT+ +GS ASP++
Sbjct: 721 EGNKATSYKFNTDEVRTAIADATKAEASAKKWLSIMSRDCNRFCKIMKTSGHGSNASPTS 780

Query: 781 IQKIKRKITFADEAGGKLCEVRLIED--HVESFVEASPEK 815
           +QK+KRKITFADEAGG+LCEVRL ED  + ES VE SPEK
Sbjct: 781 VQKLKRKITFADEAGGELCEVRLFEDDMNAESSVENSPEK 800

BLAST of MS016812 vs. NCBI nr
Match: XP_022952049.1 (uncharacterized protein LOC111454753 [Cucurbita moschata])

HSP 1 Score: 1072.0 bits (2771), Expect = 2.5e-309
Identity = 584/820 (71.22%), Postives = 666/820 (81.22%), Query Frame = 0

Query: 1   MELRSYNHFHYIHAIKGGLMTKVLNINSRGKPAVVFKKLTDIYEFIDEKDQNSLPTQLLR 60
           MELRS++HFH+IHAIKGG+MTKVLNIN RGKPA+VFKKLTDIYE I +K Q SLPT+  R
Sbjct: 1   MELRSFSHFHHIHAIKGGMMTKVLNIN-RGKPAMVFKKLTDIYESIYDKAQESLPTRWSR 60

Query: 61  ERLEENIPEGYKFKVETEDFYAERKLFKDEPTVSDSGSGG--DTDGQKSDVEVDSMTIQQ 120
           E LE NI +G + K+ET+  YAERKLF DEP VSDS   G  DT+GQKSDVE DSMTI+Q
Sbjct: 61  EGLEGNIRDGCESKMETQVLYAERKLFNDEPEVSDSDREGDSDTEGQKSDVEADSMTIKQ 120

Query: 121 IMEGCKKRKSRQSKSVDSSKEKLRTCSKQELERSCLLSDEDDDSDLNVALSIWKSKLSRR 180
           +ME CKKRK RQS SVDSSKEKLRTCS++EL  SCLLSDE DDSDL+VALSIW+SKLS+R
Sbjct: 121 MMESCKKRKVRQSNSVDSSKEKLRTCSRRELNHSCLLSDE-DDSDLDVALSIWQSKLSKR 180

Query: 181 KKLKTKCNGSRISTSSQCSQITGNSDPINSDQDLLPSSADLPIPVDIKVETPETDVTEIQ 240
           KKLK KC+ S+ISTSS   +   NSDPIN DQDLLPSS+DL IPVDIKVETPETDVT+IQ
Sbjct: 181 KKLKNKCDESKISTSSLHGRTIENSDPINIDQDLLPSSSDLAIPVDIKVETPETDVTDIQ 240

Query: 241 NTNYIIDDLSLLCDENVNSCLSSELSLLCDENVNLCLSSEPIGADELFLNRGSTTSNKEA 300
           NTN                C+  ELSLLCDENVN CLSS P G DELF     T S KEA
Sbjct: 241 NTN----------------CIPDELSLLCDENVNSCLSSGPFGTDELFFGLELTASEKEA 300

Query: 301 EYCVLNSACHEYLVGDDPEFLQMVGESNTEWMKKDNLEIQKPNYSDFPASESMEGRYAPR 360
           EY V N    E + GD+   LQMVGES+TE + +DNLE+ KP +SDFP SE+MEG+  P 
Sbjct: 301 EYGVPNCVSLENVEGDESRPLQMVGESSTECVSEDNLEVHKPQHSDFPTSETMEGQCTPS 360

Query: 361 CLSNDSMSEAISLTEEQCSGTYISQGKSITHEAICQNNCEDMSEEISPTEEQCTDTYISE 420
            +SNDS+SEAISLTEEQC GT++SQ KSITHE ICQNN EDMS  IS T EQ +DT+ISE
Sbjct: 361 FVSNDSISEAISLTEEQCLGTHMSQAKSITHEVICQNNSEDMS-AISMTGEQFSDTHISE 420

Query: 421 GKSLTHETICLNNGEVLNHLHGMTNLNSLQLPEMSFGAEVCLTENGYKDTLELDHDRKGI 480
           GK  T E IC NNGE+  +L+GM +LNSLQLPEMS GAEV LTEN YKD L  D++ KGI
Sbjct: 421 GKPFTDEAICPNNGEIFTYLNGMADLNSLQLPEMSLGAEVRLTENRYKDRLAFDNE-KGI 480

Query: 481 STEATSDCDLRADHGESISTKSTTDCNLSPDHEKSISTSSTSDGNLSPDQHLISIGKCPA 540
            TE+TSDC+L ++HG  IS+KST+DCNLSPDH +S+ST+S SD N  PDQHLISI +CPA
Sbjct: 481 PTESTSDCNLSSEHGGRISSKSTSDCNLSPDHRESVSTNSISDRNSIPDQHLISIDECPA 540

Query: 541 QEIEPQISNFSDSERNTSPDFHLDDSMDKFNQFEEPKRHPTRLLSTRTTISPTSQERLSK 600
           +E +PQ+S+ SDSERNTSPDFHL+ S +KFNQ EE +RHPTRLLSTRTTISPTSQERLSK
Sbjct: 541 KEKQPQMSDCSDSERNTSPDFHLNGSTNKFNQIEETQRHPTRLLSTRTTISPTSQERLSK 600

Query: 601 AMKSMRLHDKECKTCGGKPYFKQANYKVGTAEECDQMKRVYSDIFHEQNIRKSKKRSLHS 660
           AMKSM+L DKECKTCGGKPYFKQ  YKVGTAE CD MKRVYSD +HEQN RKSKKRSLHS
Sbjct: 601 AMKSMQLQDKECKTCGGKPYFKQIKYKVGTAEGCDPMKRVYSDTYHEQNTRKSKKRSLHS 660

Query: 661 TSNTKVPHGRT--RSTAVQSCSDNAIAFTQRQMQDIESIALKLTNQLKSMKAIVEDRLHV 720
           T+ TK  H     RS+ VQSCSD+AIAFT+RQMQDIE +ALKLT QLKSMKAIVEDR+HV
Sbjct: 661 TNTTKASHAHASMRSSTVQSCSDSAIAFTERQMQDIECLALKLTTQLKSMKAIVEDRIHV 720

Query: 721 EGNKATGFKFNTDEVRTAISDATKAEASAKKWLSMMSRDCNRFCKIMKTTENGSTASPSA 780
           EGNKAT +KFNTDEVRTAI+DATKAEASAKKWLS+MSRDCNRFCKIMKT+ +GS ASP++
Sbjct: 721 EGNKATSYKFNTDEVRTAIADATKAEASAKKWLSIMSRDCNRFCKIMKTSGHGSNASPTS 780

Query: 781 IQKIKRKITFADEAGGKLCEVRLIEDHV--ESFVEASPEK 815
           +QK+KRKITFADEAGG+LCEVRL ED +  ES VE SPEK
Sbjct: 781 VQKLKRKITFADEAGGELCEVRLFEDDMNNESSVETSPEK 800

BLAST of MS016812 vs. NCBI nr
Match: KAG7020521.1 (hypothetical protein SDJN02_17206, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1068.5 bits (2762), Expect = 2.8e-308
Identity = 583/820 (71.10%), Postives = 663/820 (80.85%), Query Frame = 0

Query: 1   MELRSYNHFHYIHAIKGGLMTKVLNINSRGKPAVVFKKLTDIYEFIDEKDQNSLPTQLLR 60
           MELRS++HFH+IHAIKGG+MTKVLNIN RGKPA+VFKKLTDIYE I +K Q SLPT+  R
Sbjct: 79  MELRSFSHFHHIHAIKGGMMTKVLNIN-RGKPAMVFKKLTDIYESIYDKAQESLPTRWSR 138

Query: 61  ERLEENIPEGYKFKVETEDFYAERKLFKDEPTVSDSGSGG--DTDGQKSDVEVDSMTIQQ 120
           E LE NI +G + K+ET+  YAERKLF DEP VSDS   G  DT+GQKSDVE DSMTI+Q
Sbjct: 139 EGLEGNIRDGCESKMETQVLYAERKLFNDEPEVSDSDREGDSDTEGQKSDVEADSMTIKQ 198

Query: 121 IMEGCKKRKSRQSKSVDSSKEKLRTCSKQELERSCLLSDEDDDSDLNVALSIWKSKLSRR 180
           +ME CKKRK RQS SVDSSKEKLRTCS++EL  SCLLSDE DDSDL+VALSIW+SKLS+R
Sbjct: 199 MMESCKKRKVRQSNSVDSSKEKLRTCSRRELNHSCLLSDE-DDSDLDVALSIWQSKLSKR 258

Query: 181 KKLKTKCNGSRISTSSQCSQITGNSDPINSDQDLLPSSADLPIPVDIKVETPETDVTEIQ 240
           KKLK KC+ S+ISTSS   Q   NSDPIN DQDLLPSS+DL IPVDIKVETPETDVTEIQ
Sbjct: 259 KKLKNKCDESKISTSSLHGQTIENSDPINIDQDLLPSSSDLAIPVDIKVETPETDVTEIQ 318

Query: 241 NTNYIIDDLSLLCDENVNSCLSSELSLLCDENVNLCLSSEPIGADELFLNRGSTTSNKEA 300
           NTN                C+  ELSLLCDENVN CLSS PIG DELF     T S KEA
Sbjct: 319 NTN----------------CIPDELSLLCDENVNSCLSSGPIGTDELFFGLELTASEKEA 378

Query: 301 EYCVLNSACHEYLVGDDPEFLQMVGESNTEWMKKDNLEIQKPNYSDFPASESMEGRYAPR 360
           EY V N    E + GD+   LQMVGES+TE + +DNLE+ KP +SDFP SE+MEG+  P 
Sbjct: 379 EYGVPNCVSLENVEGDESRPLQMVGESSTECVSEDNLEVHKPQHSDFPTSETMEGQCTPS 438

Query: 361 CLSNDSMSEAISLTEEQCSGTYISQGKSITHEAICQNNCEDMSEEISPTEEQCTDTYISE 420
            +SNDS+SEAISLTEEQC G ++SQ KSITHE ICQNN EDMS  IS T EQ +DT+ISE
Sbjct: 439 FVSNDSISEAISLTEEQCLGIHMSQAKSITHEVICQNNSEDMS-AISMTGEQFSDTHISE 498

Query: 421 GKSLTHETICLNNGEVLNHLHGMTNLNSLQLPEMSFGAEVCLTENGYKDTLELDHDRKGI 480
           GK  T E IC  NGE+  +L+GM +LNSLQLPEMS GAE  LTEN YKD L  D++ KGI
Sbjct: 499 GKPFTDEAICPTNGEIFTYLNGMADLNSLQLPEMSLGAEARLTENRYKDRLAFDNE-KGI 558

Query: 481 STEATSDCDLRADHGESISTKSTTDCNLSPDHEKSISTSSTSDGNLSPDQHLISIGKCPA 540
            TE+T DC+L ++HG  IS+KST+DCNLSPDH +S+ST+S SD N  PDQHLISI +CPA
Sbjct: 559 PTESTGDCNLSSEHGGRISSKSTSDCNLSPDHGESVSTNSISDRNSIPDQHLISIDECPA 618

Query: 541 QEIEPQISNFSDSERNTSPDFHLDDSMDKFNQFEEPKRHPTRLLSTRTTISPTSQERLSK 600
           +E +PQ+S+ SDSERNTSPDFHL+ S DKFNQ EE +RHPTRLLSTRTTISPTSQERLSK
Sbjct: 619 KEKQPQMSDCSDSERNTSPDFHLNGSTDKFNQIEETQRHPTRLLSTRTTISPTSQERLSK 678

Query: 601 AMKSMRLHDKECKTCGGKPYFKQANYKVGTAEECDQMKRVYSDIFHEQNIRKSKKRSLHS 660
           AMKSM+L DKECKTCGGKPYFKQ  YKVGTAE CD MKRVYSD +HEQN RKSKKRSLHS
Sbjct: 679 AMKSMQLQDKECKTCGGKPYFKQIKYKVGTAEGCDPMKRVYSDTYHEQNTRKSKKRSLHS 738

Query: 661 TSNTKVPHGRT--RSTAVQSCSDNAIAFTQRQMQDIESIALKLTNQLKSMKAIVEDRLHV 720
           T+ TK  H     RS+ VQSCSD+AIAFT+RQMQDIE +ALKLT QLKSMKAIVEDR+HV
Sbjct: 739 TNTTKASHAHASMRSSTVQSCSDSAIAFTERQMQDIECLALKLTTQLKSMKAIVEDRIHV 798

Query: 721 EGNKATGFKFNTDEVRTAISDATKAEASAKKWLSMMSRDCNRFCKIMKTTENGSTASPSA 780
           EGNKAT +KFNTDEVRTAI+DATKAEASAKKWLS+MSRDCNRFCKIMKT+ +GS ASP++
Sbjct: 799 EGNKATSYKFNTDEVRTAIADATKAEASAKKWLSIMSRDCNRFCKIMKTSGHGSNASPTS 858

Query: 781 IQKIKRKITFADEAGGKLCEVRLIED--HVESFVEASPEK 815
           +QK+KRKITFADEAGG+LCEVRL ED  + ES VE SP+K
Sbjct: 859 VQKLKRKITFADEAGGELCEVRLFEDDMNAESSVETSPKK 878

BLAST of MS016812 vs. NCBI nr
Match: XP_023002800.1 (uncharacterized protein LOC111496554 [Cucurbita maxima])

HSP 1 Score: 1057.4 bits (2733), Expect = 6.4e-305
Identity = 581/820 (70.85%), Postives = 660/820 (80.49%), Query Frame = 0

Query: 1   MELRSYNHFHYIHAIKGGLMTKVLNINSRGKPAVVFKKLTDIYEFIDEKDQNSLPTQLLR 60
           MELRS++HFH+IHAIKGG+M KVLNIN RGKPA+VFKKLTDIYE I +K Q SLPT+  R
Sbjct: 1   MELRSFSHFHHIHAIKGGMMPKVLNIN-RGKPAMVFKKLTDIYESIYDKAQESLPTRWSR 60

Query: 61  ERLEENIPEGYKFKVETEDFYAERKLFKDEPTVSDSGSGGDTDG--QKSDVEVDSMTIQQ 120
           E LE NI +G + K++T+  YAERKLF DEP VSDS   GD+D   QKSDVE DSMTI+Q
Sbjct: 61  EGLEGNIRDGCESKMQTQVLYAERKLFNDEPEVSDSDREGDSDTEVQKSDVEADSMTIKQ 120

Query: 121 IMEGCKKRKSRQSKSVDSSKEKLRTCSKQELERSCLLSDEDDDSDLNVALSIWKSKLSRR 180
           +ME CKKRK RQS  VDSSKEK RTCS++EL  SCLLSDE DDSDL+VALSIW+SKLS+R
Sbjct: 121 MMESCKKRKLRQSNFVDSSKEKPRTCSRRELNHSCLLSDE-DDSDLDVALSIWQSKLSKR 180

Query: 181 KKLKTKCNGSRISTSSQCSQITGNSDPINSDQDLLPSSADLPIPVDIKVETPETDVTEIQ 240
           KKLKTKC+ S+ISTSS   Q   NSDPIN DQDLLPSS+DL IPVDIKVETPETDVTEIQ
Sbjct: 181 KKLKTKCDESKISTSSLQGQTIENSDPINIDQDLLPSSSDLAIPVDIKVETPETDVTEIQ 240

Query: 241 NTNYIIDDLSLLCDENVNSCLSSELSLLCDENVNLCLSSEPIGADELFLNRGSTTSNKEA 300
           NTN                C++ ELSLLCDENVN CLSS PIG DELF     T S KE 
Sbjct: 241 NTN----------------CITVELSLLCDENVNSCLSSGPIGTDELFFGLELTASEKEP 300

Query: 301 EYCVLNSACHEYLVGDDPEFLQMVGESNTEWMKKDNLEIQKPNYSDFPASESMEGRYAPR 360
           EY VLN    E +  D+   LQMVGES+TE + +DNLE+ KP +SDFPASE+MEG+  P 
Sbjct: 301 EYGVLNYVSLENVECDESRPLQMVGESSTECVSEDNLEVHKPQHSDFPASETMEGQCTPS 360

Query: 361 CLSNDSMSEAISLTEEQCSGTYISQGKSITHEAICQNNCEDMSEEISPTEEQCTDTYISE 420
            +SNDS+SEAISL EEQC G +ISQ KSITHE ICQ N EDMS  IS T EQ +DT+ISE
Sbjct: 361 FVSNDSISEAISLAEEQCFGIHISQAKSITHEVICQKNSEDMS-AISMTGEQFSDTHISE 420

Query: 421 GKSLTHETICLNNGEVLNHLHGMTNLNSLQLPEMSFGAEVCLTENGYKDTLELDHDRKGI 480
           GK  T E IC  NG +  +L+GM +LNSLQLPEMS GAEV LTEN YKD L  D++ KGI
Sbjct: 421 GKPFTDEVICPTNGVIFTYLNGMADLNSLQLPEMSLGAEVRLTENRYKDRLAFDNE-KGI 480

Query: 481 STEATSDCDLRADHGESISTKSTTDCNLSPDHEKSISTSSTSDGNLSPDQHLISIGKCPA 540
            TE+TSDC+L ++HG SIS+KST+DCNLSPDH +S+ST+S SD NL PDQHLISI +CPA
Sbjct: 481 PTESTSDCNLSSEHGGSISSKSTSDCNLSPDHGESVSTNSISDRNLIPDQHLISIDECPA 540

Query: 541 QEIEPQISNFSDSERNTSPDFHLDDSMDKFNQFEEPKRHPTRLLSTRTTISPTSQERLSK 600
           +E +PQ+S+ SD ERNTSPDFHL+ S DKFNQ EEP+RHPTRL STRTTISPTSQERLSK
Sbjct: 541 KEKQPQMSDCSDPERNTSPDFHLNGSTDKFNQIEEPQRHPTRLQSTRTTISPTSQERLSK 600

Query: 601 AMKSMRLHDKECKTCGGKPYFKQANYKVGTAEECDQMKRVYSDIFHEQNIRKSKKRSLHS 660
           AMKSM+L DKECKTCGGKPYFKQ  YKVGTAE CD MKRVYSD +HEQN RKSKKRSLHS
Sbjct: 601 AMKSMQLQDKECKTCGGKPYFKQIKYKVGTAEGCDPMKRVYSDTYHEQNTRKSKKRSLHS 660

Query: 661 TSNTKVPHGRT--RSTAVQSCSDNAIAFTQRQMQDIESIALKLTNQLKSMKAIVEDRLHV 720
           TS TK  H     RS+ VQSCS++AIAFT+RQMQDIE +ALKLT QLKSMKAIVEDR+HV
Sbjct: 661 TSTTKASHAHASMRSSTVQSCSESAIAFTERQMQDIECLALKLTTQLKSMKAIVEDRIHV 720

Query: 721 EGNKATGFKFNTDEVRTAISDATKAEASAKKWLSMMSRDCNRFCKIMKTTENGSTASPSA 780
           EGNKAT +KFNTDEVRTAI+DATKAEASAKKWLS+MSRDCNRFCKIMKT+ +GS ASP++
Sbjct: 721 EGNKATSYKFNTDEVRTAIADATKAEASAKKWLSIMSRDCNRFCKIMKTSGHGSNASPTS 780

Query: 781 IQKIKRKITFADEAGGKLCEVRLIED--HVESFVEASPEK 815
            QK+KRKITFADEAGG+LCEVRL ED  + ES VE SPEK
Sbjct: 781 AQKLKRKITFADEAGGELCEVRLFEDDMNAESSVETSPEK 800

BLAST of MS016812 vs. ExPASy TrEMBL
Match: A0A6J1CT21 (uncharacterized protein LOC111014058 OS=Momordica charantia OX=3673 GN=LOC111014058 PE=4 SV=1)

HSP 1 Score: 1513.8 bits (3918), Expect = 0.0e+00
Identity = 782/819 (95.48%), Postives = 786/819 (95.97%), Query Frame = 0

Query: 1   MELRSYNHFHYIHAIKGGLMTKVLNINSRGKPAVVFKKLTDIYEFIDEKDQNSLPTQLLR 60
           MELRSYNHFHYIHAIKGGLMTKVLNINSRGKPAVVFKKLTD+YEFIDEKDQNSLPTQLLR
Sbjct: 1   MELRSYNHFHYIHAIKGGLMTKVLNINSRGKPAVVFKKLTDVYEFIDEKDQNSLPTQLLR 60

Query: 61  ERLEENIPEGYKFKVETEDFYAERKLFKDEPTVSDSGSGGDTDGQKSDVEVDSMTIQQIM 120
           ERLEENIPEGYKFKVETEDFYAERKLFKDEPTVSDSGSGGDTDGQKSDVEVDSMTIQQIM
Sbjct: 61  ERLEENIPEGYKFKVETEDFYAERKLFKDEPTVSDSGSGGDTDGQKSDVEVDSMTIQQIM 120

Query: 121 EGCKKRKSRQSKSVDSSKEKLRTCSKQELERSCLLSDEDDDSDLNVALSIWKSKLSRRKK 180
           EGCKKRKSRQSKSVDSSKEKLRTCSKQELERSCLLSDEDDDSDLNVALS+WKSKLSRRKK
Sbjct: 121 EGCKKRKSRQSKSVDSSKEKLRTCSKQELERSCLLSDEDDDSDLNVALSVWKSKLSRRKK 180

Query: 181 LKTKCNGSRISTSSQCSQITGNSDPINSDQDLLPSSADLPIPVDIKVETPETDVTEIQNT 240
           LKTKCNGSRISTSSQCSQITGNSDPINSDQDLLPSSADLPIPVD+KVETPETDVTEIQNT
Sbjct: 181 LKTKCNGSRISTSSQCSQITGNSDPINSDQDLLPSSADLPIPVDVKVETPETDVTEIQNT 240

Query: 241 NYIIDDLSLLCDENVNSCLSSELSLLCDENVNLCLSSEPIGADELFLNRGSTTSNKEAEY 300
           NYIIDDLSLLCDENVNSCLSSELSLLCDENVNLCLSSEPIGADELFLNRGSTTSNKEAEY
Sbjct: 241 NYIIDDLSLLCDENVNSCLSSELSLLCDENVNLCLSSEPIGADELFLNRGSTTSNKEAEY 300

Query: 301 CVLNSACHEYLVGDDPEFLQMVGESNTEWMKKDNLEIQKPNYSDFPASESMEGRYAPRCL 360
           CVLNSACHEYLVGDDPEFLQMVGESNTEWMKKDNLEIQKPNYSDFPASESMEGRYAPRCL
Sbjct: 301 CVLNSACHEYLVGDDPEFLQMVGESNTEWMKKDNLEIQKPNYSDFPASESMEGRYAPRCL 360

Query: 361 SNDSMSEAISLTEEQCSGTYISQGKSITHEAICQNNCEDMSEEISPTEEQCTDTYISEGK 420
           SNDSMSE ISLTEEQCSGTYISQGKSITHEAICQNNCEDMSEEISPTEEQCTDTYISE  
Sbjct: 361 SNDSMSEEISLTEEQCSGTYISQGKSITHEAICQNNCEDMSEEISPTEEQCTDTYISE-- 420

Query: 421 SLTHETICLNNGEVLNHLHGMTNLNSLQLPEMSFGAEVCLTENGYKDTLELDHDRKGIST 480
                                         EMSFGAEVCLTENGYKDTLELDH+RKGIST
Sbjct: 421 ------------------------------EMSFGAEVCLTENGYKDTLELDHERKGIST 480

Query: 481 EATSDCDLRADHGESISTKSTTDCNLSPDHEKSISTSSTSDGNLSPDQHLISIGKCPAQE 540
           EATSDCDLRADHGESISTKSTTDCNLSPDHEKSISTSSTSDGNLSPDQHLISIGKCPAQE
Sbjct: 481 EATSDCDLRADHGESISTKSTTDCNLSPDHEKSISTSSTSDGNLSPDQHLISIGKCPAQE 540

Query: 541 IEPQISNFSDSERNTSPDFHLDDSMDKFNQFEEPKRHPTRLLSTRTTISPTSQERLSKAM 600
           IEPQISNFSDSERNTSPDFHLDDSMDKFNQFEEPKRHPTRLLSTRTTISPTSQERLSKAM
Sbjct: 541 IEPQISNFSDSERNTSPDFHLDDSMDKFNQFEEPKRHPTRLLSTRTTISPTSQERLSKAM 600

Query: 601 KSMRLHDKECKTCGGKPYFKQANYKVGTAEECDQMKRVYSDIFHEQNIRKSKKRSLHSTS 660
           KSMRLHDKECKTCGGKPYFKQANYKVGTAEECDQMKRVYSDIFHEQNIRKSKKRSLHSTS
Sbjct: 601 KSMRLHDKECKTCGGKPYFKQANYKVGTAEECDQMKRVYSDIFHEQNIRKSKKRSLHSTS 660

Query: 661 NTKVPHGRTRSTAVQSCSDNAIAFTQRQMQDIESIALKLTNQLKSMKAIVEDRLHVEGNK 720
           NTKVPHGRTRSTAVQSCSDNAIAFTQRQMQDIESIALKLTNQLKSMKAIVEDRLHVEGNK
Sbjct: 661 NTKVPHGRTRSTAVQSCSDNAIAFTQRQMQDIESIALKLTNQLKSMKAIVEDRLHVEGNK 720

Query: 721 ATGFKFNTDEVRTAISDATKAEASAKKWLSMMSRDCNRFCKIMKTTENGSTASPSAIQKI 780
           ATGFKFNTDEVRTAISDATKAEASAKKWLSMMSRDCNRFCKIMKTTENGSTASPSAIQKI
Sbjct: 721 ATGFKFNTDEVRTAISDATKAEASAKKWLSMMSRDCNRFCKIMKTTENGSTASPSAIQKI 780

Query: 781 KRKITFADEAGGKLCEVRLIEDHVESFVEASPEKYEAVK 820
           KRKITFADEAGGKLCEVRLIEDHVESFVEASPEKYEAVK
Sbjct: 781 KRKITFADEAGGKLCEVRLIEDHVESFVEASPEKYEAVK 787

BLAST of MS016812 vs. ExPASy TrEMBL
Match: A0A6J1GKI4 (uncharacterized protein LOC111454753 OS=Cucurbita moschata OX=3662 GN=LOC111454753 PE=4 SV=1)

HSP 1 Score: 1072.0 bits (2771), Expect = 1.2e-309
Identity = 584/820 (71.22%), Postives = 666/820 (81.22%), Query Frame = 0

Query: 1   MELRSYNHFHYIHAIKGGLMTKVLNINSRGKPAVVFKKLTDIYEFIDEKDQNSLPTQLLR 60
           MELRS++HFH+IHAIKGG+MTKVLNIN RGKPA+VFKKLTDIYE I +K Q SLPT+  R
Sbjct: 1   MELRSFSHFHHIHAIKGGMMTKVLNIN-RGKPAMVFKKLTDIYESIYDKAQESLPTRWSR 60

Query: 61  ERLEENIPEGYKFKVETEDFYAERKLFKDEPTVSDSGSGG--DTDGQKSDVEVDSMTIQQ 120
           E LE NI +G + K+ET+  YAERKLF DEP VSDS   G  DT+GQKSDVE DSMTI+Q
Sbjct: 61  EGLEGNIRDGCESKMETQVLYAERKLFNDEPEVSDSDREGDSDTEGQKSDVEADSMTIKQ 120

Query: 121 IMEGCKKRKSRQSKSVDSSKEKLRTCSKQELERSCLLSDEDDDSDLNVALSIWKSKLSRR 180
           +ME CKKRK RQS SVDSSKEKLRTCS++EL  SCLLSDE DDSDL+VALSIW+SKLS+R
Sbjct: 121 MMESCKKRKVRQSNSVDSSKEKLRTCSRRELNHSCLLSDE-DDSDLDVALSIWQSKLSKR 180

Query: 181 KKLKTKCNGSRISTSSQCSQITGNSDPINSDQDLLPSSADLPIPVDIKVETPETDVTEIQ 240
           KKLK KC+ S+ISTSS   +   NSDPIN DQDLLPSS+DL IPVDIKVETPETDVT+IQ
Sbjct: 181 KKLKNKCDESKISTSSLHGRTIENSDPINIDQDLLPSSSDLAIPVDIKVETPETDVTDIQ 240

Query: 241 NTNYIIDDLSLLCDENVNSCLSSELSLLCDENVNLCLSSEPIGADELFLNRGSTTSNKEA 300
           NTN                C+  ELSLLCDENVN CLSS P G DELF     T S KEA
Sbjct: 241 NTN----------------CIPDELSLLCDENVNSCLSSGPFGTDELFFGLELTASEKEA 300

Query: 301 EYCVLNSACHEYLVGDDPEFLQMVGESNTEWMKKDNLEIQKPNYSDFPASESMEGRYAPR 360
           EY V N    E + GD+   LQMVGES+TE + +DNLE+ KP +SDFP SE+MEG+  P 
Sbjct: 301 EYGVPNCVSLENVEGDESRPLQMVGESSTECVSEDNLEVHKPQHSDFPTSETMEGQCTPS 360

Query: 361 CLSNDSMSEAISLTEEQCSGTYISQGKSITHEAICQNNCEDMSEEISPTEEQCTDTYISE 420
            +SNDS+SEAISLTEEQC GT++SQ KSITHE ICQNN EDMS  IS T EQ +DT+ISE
Sbjct: 361 FVSNDSISEAISLTEEQCLGTHMSQAKSITHEVICQNNSEDMS-AISMTGEQFSDTHISE 420

Query: 421 GKSLTHETICLNNGEVLNHLHGMTNLNSLQLPEMSFGAEVCLTENGYKDTLELDHDRKGI 480
           GK  T E IC NNGE+  +L+GM +LNSLQLPEMS GAEV LTEN YKD L  D++ KGI
Sbjct: 421 GKPFTDEAICPNNGEIFTYLNGMADLNSLQLPEMSLGAEVRLTENRYKDRLAFDNE-KGI 480

Query: 481 STEATSDCDLRADHGESISTKSTTDCNLSPDHEKSISTSSTSDGNLSPDQHLISIGKCPA 540
            TE+TSDC+L ++HG  IS+KST+DCNLSPDH +S+ST+S SD N  PDQHLISI +CPA
Sbjct: 481 PTESTSDCNLSSEHGGRISSKSTSDCNLSPDHRESVSTNSISDRNSIPDQHLISIDECPA 540

Query: 541 QEIEPQISNFSDSERNTSPDFHLDDSMDKFNQFEEPKRHPTRLLSTRTTISPTSQERLSK 600
           +E +PQ+S+ SDSERNTSPDFHL+ S +KFNQ EE +RHPTRLLSTRTTISPTSQERLSK
Sbjct: 541 KEKQPQMSDCSDSERNTSPDFHLNGSTNKFNQIEETQRHPTRLLSTRTTISPTSQERLSK 600

Query: 601 AMKSMRLHDKECKTCGGKPYFKQANYKVGTAEECDQMKRVYSDIFHEQNIRKSKKRSLHS 660
           AMKSM+L DKECKTCGGKPYFKQ  YKVGTAE CD MKRVYSD +HEQN RKSKKRSLHS
Sbjct: 601 AMKSMQLQDKECKTCGGKPYFKQIKYKVGTAEGCDPMKRVYSDTYHEQNTRKSKKRSLHS 660

Query: 661 TSNTKVPHGRT--RSTAVQSCSDNAIAFTQRQMQDIESIALKLTNQLKSMKAIVEDRLHV 720
           T+ TK  H     RS+ VQSCSD+AIAFT+RQMQDIE +ALKLT QLKSMKAIVEDR+HV
Sbjct: 661 TNTTKASHAHASMRSSTVQSCSDSAIAFTERQMQDIECLALKLTTQLKSMKAIVEDRIHV 720

Query: 721 EGNKATGFKFNTDEVRTAISDATKAEASAKKWLSMMSRDCNRFCKIMKTTENGSTASPSA 780
           EGNKAT +KFNTDEVRTAI+DATKAEASAKKWLS+MSRDCNRFCKIMKT+ +GS ASP++
Sbjct: 721 EGNKATSYKFNTDEVRTAIADATKAEASAKKWLSIMSRDCNRFCKIMKTSGHGSNASPTS 780

Query: 781 IQKIKRKITFADEAGGKLCEVRLIEDHV--ESFVEASPEK 815
           +QK+KRKITFADEAGG+LCEVRL ED +  ES VE SPEK
Sbjct: 781 VQKLKRKITFADEAGGELCEVRLFEDDMNNESSVETSPEK 800

BLAST of MS016812 vs. ExPASy TrEMBL
Match: A0A6J1KPZ6 (uncharacterized protein LOC111496554 OS=Cucurbita maxima OX=3661 GN=LOC111496554 PE=4 SV=1)

HSP 1 Score: 1057.4 bits (2733), Expect = 3.1e-305
Identity = 581/820 (70.85%), Postives = 660/820 (80.49%), Query Frame = 0

Query: 1   MELRSYNHFHYIHAIKGGLMTKVLNINSRGKPAVVFKKLTDIYEFIDEKDQNSLPTQLLR 60
           MELRS++HFH+IHAIKGG+M KVLNIN RGKPA+VFKKLTDIYE I +K Q SLPT+  R
Sbjct: 1   MELRSFSHFHHIHAIKGGMMPKVLNIN-RGKPAMVFKKLTDIYESIYDKAQESLPTRWSR 60

Query: 61  ERLEENIPEGYKFKVETEDFYAERKLFKDEPTVSDSGSGGDTDG--QKSDVEVDSMTIQQ 120
           E LE NI +G + K++T+  YAERKLF DEP VSDS   GD+D   QKSDVE DSMTI+Q
Sbjct: 61  EGLEGNIRDGCESKMQTQVLYAERKLFNDEPEVSDSDREGDSDTEVQKSDVEADSMTIKQ 120

Query: 121 IMEGCKKRKSRQSKSVDSSKEKLRTCSKQELERSCLLSDEDDDSDLNVALSIWKSKLSRR 180
           +ME CKKRK RQS  VDSSKEK RTCS++EL  SCLLSDE DDSDL+VALSIW+SKLS+R
Sbjct: 121 MMESCKKRKLRQSNFVDSSKEKPRTCSRRELNHSCLLSDE-DDSDLDVALSIWQSKLSKR 180

Query: 181 KKLKTKCNGSRISTSSQCSQITGNSDPINSDQDLLPSSADLPIPVDIKVETPETDVTEIQ 240
           KKLKTKC+ S+ISTSS   Q   NSDPIN DQDLLPSS+DL IPVDIKVETPETDVTEIQ
Sbjct: 181 KKLKTKCDESKISTSSLQGQTIENSDPINIDQDLLPSSSDLAIPVDIKVETPETDVTEIQ 240

Query: 241 NTNYIIDDLSLLCDENVNSCLSSELSLLCDENVNLCLSSEPIGADELFLNRGSTTSNKEA 300
           NTN                C++ ELSLLCDENVN CLSS PIG DELF     T S KE 
Sbjct: 241 NTN----------------CITVELSLLCDENVNSCLSSGPIGTDELFFGLELTASEKEP 300

Query: 301 EYCVLNSACHEYLVGDDPEFLQMVGESNTEWMKKDNLEIQKPNYSDFPASESMEGRYAPR 360
           EY VLN    E +  D+   LQMVGES+TE + +DNLE+ KP +SDFPASE+MEG+  P 
Sbjct: 301 EYGVLNYVSLENVECDESRPLQMVGESSTECVSEDNLEVHKPQHSDFPASETMEGQCTPS 360

Query: 361 CLSNDSMSEAISLTEEQCSGTYISQGKSITHEAICQNNCEDMSEEISPTEEQCTDTYISE 420
            +SNDS+SEAISL EEQC G +ISQ KSITHE ICQ N EDMS  IS T EQ +DT+ISE
Sbjct: 361 FVSNDSISEAISLAEEQCFGIHISQAKSITHEVICQKNSEDMS-AISMTGEQFSDTHISE 420

Query: 421 GKSLTHETICLNNGEVLNHLHGMTNLNSLQLPEMSFGAEVCLTENGYKDTLELDHDRKGI 480
           GK  T E IC  NG +  +L+GM +LNSLQLPEMS GAEV LTEN YKD L  D++ KGI
Sbjct: 421 GKPFTDEVICPTNGVIFTYLNGMADLNSLQLPEMSLGAEVRLTENRYKDRLAFDNE-KGI 480

Query: 481 STEATSDCDLRADHGESISTKSTTDCNLSPDHEKSISTSSTSDGNLSPDQHLISIGKCPA 540
            TE+TSDC+L ++HG SIS+KST+DCNLSPDH +S+ST+S SD NL PDQHLISI +CPA
Sbjct: 481 PTESTSDCNLSSEHGGSISSKSTSDCNLSPDHGESVSTNSISDRNLIPDQHLISIDECPA 540

Query: 541 QEIEPQISNFSDSERNTSPDFHLDDSMDKFNQFEEPKRHPTRLLSTRTTISPTSQERLSK 600
           +E +PQ+S+ SD ERNTSPDFHL+ S DKFNQ EEP+RHPTRL STRTTISPTSQERLSK
Sbjct: 541 KEKQPQMSDCSDPERNTSPDFHLNGSTDKFNQIEEPQRHPTRLQSTRTTISPTSQERLSK 600

Query: 601 AMKSMRLHDKECKTCGGKPYFKQANYKVGTAEECDQMKRVYSDIFHEQNIRKSKKRSLHS 660
           AMKSM+L DKECKTCGGKPYFKQ  YKVGTAE CD MKRVYSD +HEQN RKSKKRSLHS
Sbjct: 601 AMKSMQLQDKECKTCGGKPYFKQIKYKVGTAEGCDPMKRVYSDTYHEQNTRKSKKRSLHS 660

Query: 661 TSNTKVPHGRT--RSTAVQSCSDNAIAFTQRQMQDIESIALKLTNQLKSMKAIVEDRLHV 720
           TS TK  H     RS+ VQSCS++AIAFT+RQMQDIE +ALKLT QLKSMKAIVEDR+HV
Sbjct: 661 TSTTKASHAHASMRSSTVQSCSESAIAFTERQMQDIECLALKLTTQLKSMKAIVEDRIHV 720

Query: 721 EGNKATGFKFNTDEVRTAISDATKAEASAKKWLSMMSRDCNRFCKIMKTTENGSTASPSA 780
           EGNKAT +KFNTDEVRTAI+DATKAEASAKKWLS+MSRDCNRFCKIMKT+ +GS ASP++
Sbjct: 721 EGNKATSYKFNTDEVRTAIADATKAEASAKKWLSIMSRDCNRFCKIMKTSGHGSNASPTS 780

Query: 781 IQKIKRKITFADEAGGKLCEVRLIED--HVESFVEASPEK 815
            QK+KRKITFADEAGG+LCEVRL ED  + ES VE SPEK
Sbjct: 781 AQKLKRKITFADEAGGELCEVRLFEDDMNAESSVETSPEK 800

BLAST of MS016812 vs. ExPASy TrEMBL
Match: A0A5A7VDU2 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G005880 PE=4 SV=1)

HSP 1 Score: 1016.9 bits (2628), Expect = 4.7e-293
Identity = 550/821 (66.99%), Postives = 639/821 (77.83%), Query Frame = 0

Query: 1   MELRSYNHFHYIHAIKGGLMTKVLNINSRGKPAVVFKKLTDIYEFIDEKDQNSLPTQLLR 60
           MELRS+ H HYIHAIKGG++ K+LNIN RGKPAVVFKKLTDIY  ID++ +  LPT+  R
Sbjct: 1   MELRSFCHLHYIHAIKGGVVNKILNIN-RGKPAVVFKKLTDIYGSIDDEAREPLPTRGSR 60

Query: 61  ERLEENIPEGYKFKVETEDFYAERKLFKDEPTVSDSGSGGDTDGQKSDVEVDSMTIQQIM 120
           E LEEN   G +FKV+++  YAER L  DEP +SDS S GD+D QKSD EVDSMT++QIM
Sbjct: 61  EGLEENTLHGCEFKVDSQVLYAERTLCNDEPEISDSDSKGDSDVQKSDFEVDSMTLKQIM 120

Query: 121 EGCKKRKSRQSKSVDSSKEKLRTCSKQELERSCLLSDEDDDSDLNVALSIWKSKLSRRKK 180
           EGCKKRK  QS+ VDSSKEK RTC KQEL+ S +L+ E+DDSDLN+ALSIWKSKLS+R+K
Sbjct: 121 EGCKKRKLSQSRFVDSSKEKTRTCFKQELDHSFMLT-EEDDSDLNIALSIWKSKLSKRRK 180

Query: 181 LKTKCNGSRISTSSQCSQITGNSDPINSDQDLLPSSADLPIPVDIKVETPETDVTEIQNT 240
           LKTKC  SRISTSSQC Q  G+SDP +SD+DLLPS ++LP+PVD+KVETPE DVTEIQNT
Sbjct: 181 LKTKCEESRISTSSQCDQTIGSSDPTDSDEDLLPSGSNLPLPVDVKVETPEIDVTEIQNT 240

Query: 241 NYIIDDLSLLCDENVNSCLSSELSLLCDENVNLCLSSEPIGADELFLNRGSTTSNKEAEY 300
           N+ I+                E SL CDEN+N CLS  P+G ++L L+ G T S KEAEY
Sbjct: 241 NHTIE----------------ECSLFCDENINFCLSYGPVGPNDLNLDIGLTASEKEAEY 300

Query: 301 CVLNSACHEYLVGDDPEFLQMVGESNTEWMKKDNLEIQKPNYSDFPASESMEGRYAPRCL 360
           CVLNSAC+EY  G +P   QMVGES+T+WM +D LE+ K ++SDF AS+SM+G+Y P  +
Sbjct: 301 CVLNSACYEYFEGYEPGTFQMVGESSTKWMNEDKLEVHKTHHSDFSASDSMKGQYTPSYI 360

Query: 361 SNDSMSEAISLTEEQCSGTYISQGKSITHEAICQNNCEDMSEEISPTEEQCTDTYISEGK 420
           SN S+SEAI LT+EQCSG+YIS   SIT+ A+CQN+ + MSE I+ TEEQC DTYISEGK
Sbjct: 361 SNSSISEAIPLTKEQCSGSYISPDNSITNVAMCQNSSKGMSEVIALTEEQCCDTYISEGK 420

Query: 421 SLTHETICLNNGEVLNHLHGMTNLNSLQLPEMSFGAEVCLTENGYKDTLELDHDRKGIST 480
            LTHE  CLNNGE   H+H +TNLNSL+ PEMS GAEVCLTEN YKD L +D +R     
Sbjct: 421 PLTHEATCLNNGEGSTHMHALTNLNSLEAPEMSHGAEVCLTENSYKDELAVDDER----- 480

Query: 481 EATSDCDLRADHGESISTKSTTDCNLSPDHEKSISTSSTSDGNLSPDQHLISIGKCPAQE 540
                         SI  KS+ D NLSPDH K ISTSS SD N   DQ LIS  +CPA+E
Sbjct: 481 --------------SIPKKSSCDSNLSPDHGKYISTSSISDRNSGSDQRLISDDECPAKE 540

Query: 541 IEPQISNFSDSERNTSPDFHLDDSMDKFNQFEEPKRHPTRLLSTRTTISPTSQERLSKAM 600
            +PQ+S+ SDSERNTSP+ HLD S+DKFNQFEEPKRHPTRLLSTRTTISPTSQERLSKAM
Sbjct: 541 RQPQMSDCSDSERNTSPNSHLDGSVDKFNQFEEPKRHPTRLLSTRTTISPTSQERLSKAM 600

Query: 601 KSMRLHDKECKTCGGKPYFKQANYKVGTAEECDQMKRVYSDIFHEQNIRKSKKRSLHSTS 660
           KSMRLHDKECKT G KP+FKQ  Y VG AEECDQ K+V+SDI+ E+NIRKSKKRS HS+S
Sbjct: 601 KSMRLHDKECKTYGVKPHFKQTKYAVGAAEECDQTKQVHSDIYQEKNIRKSKKRSFHSSS 660

Query: 661 NTKVPHGRTRSTAVQSCSDNAIAFTQRQMQDIESIALKLTNQLKSMKAIVEDRLHVEGNK 720
            TKVP        VQ+CS++AI FTQRQMQDIE +ALKLT+QLKSMKAIVEDRLHVEGNK
Sbjct: 661 TTKVP-----QATVQNCSESAITFTQRQMQDIECLALKLTSQLKSMKAIVEDRLHVEGNK 720

Query: 721 ATGFKFNTDEVRTAISDATKAEASAKKWLSMMSRDCNRFCKIMKTTENGSTASPSAIQKI 780
           +T FKFN DEVRTAI+DATKAE SAKKWLS+MSRDCNRFCKIM TTE+ S ASP+AIQK 
Sbjct: 721 STSFKFNADEVRTAIADATKAETSAKKWLSIMSRDCNRFCKIMNTTEHYSNASPAAIQKA 779

Query: 781 KRKITFADEAGGKLCEVRLIEDHV--ESFVEASPEKYEAVK 820
           KRK+TFADEAGGKLCEVRLIED V  ES  E SPE  E  +
Sbjct: 781 KRKVTFADEAGGKLCEVRLIEDDVNAESSAEMSPENCETAQ 779

BLAST of MS016812 vs. ExPASy TrEMBL
Match: A0A6J1K4P1 (uncharacterized protein LOC111492272 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111492272 PE=4 SV=1)

HSP 1 Score: 1016.5 bits (2627), Expect = 6.1e-293
Identity = 554/810 (68.40%), Postives = 630/810 (77.78%), Query Frame = 0

Query: 1   MELRSYNHFHYIHAIKGGLMTKVLNINSRGKPAVVFKKLTDIYEFIDEKDQNSLPTQLLR 60
           MELRS++H HYI+  KGG M+KVLN+NS GKPAVVFKKLTDIYE ID+K Q SLP +  R
Sbjct: 1   MELRSFSHLHYINVTKGGAMSKVLNVNSHGKPAVVFKKLTDIYESIDDKTQESLPRRWSR 60

Query: 61  ERLEENIPEGYKFKVETEDFYAERKLFKDEPTVSDSGSGGDTDGQKSDVEVDSMTIQQIM 120
           E LEENIP+  +FKVET+  YAERKLF +EP VSDS S GDTDGQKSDVEVDSMT++QI 
Sbjct: 61  EGLEENIPDECEFKVETQVLYAERKLFNNEPEVSDSDSKGDTDGQKSDVEVDSMTLKQIT 120

Query: 121 EGCKKRKSRQSKSVDSSKEKLRTCSKQELERSCLLSDEDDDSDLNVALSIWKSKLSRRKK 180
           EGCKKRK RQS+SVDSSKEKLRTCS++EL+ +CLLSDE DDSDLNVAL+IWKSKLS+R+K
Sbjct: 121 EGCKKRKLRQSRSVDSSKEKLRTCSRRELDHACLLSDE-DDSDLNVALNIWKSKLSKRRK 180

Query: 181 LKTKCNGSRISTSSQCSQITGNSDPINSDQDLLPSSADLPIPVDIKVETPETDVTEIQNT 240
           LKTKC+ SRISTSS C Q  GNSDPINSDQDL PS +DLP+PVDIKVETPE DV+EIQ+T
Sbjct: 181 LKTKCDESRISTSSHCGQTIGNSDPINSDQDLHPSGSDLPVPVDIKVETPEPDVSEIQST 240

Query: 241 NYIIDDLSLLCDENVNSCLSSELSLLCDENVNLCLSSEPIGADELFLNRGSTTSNKEAEY 300
           NY ID+ SL CDEN+NS                CL   P GADE       TTS KEAEY
Sbjct: 241 NYKIDEWSLFCDENINS----------------CLKHGPNGADESIFYPKLTTSEKEAEY 300

Query: 301 CVLNSACHEYLVGDDPEFLQMVGESNTEWMKKDNLEIQKPNYSDFPASESMEGRYAPRCL 360
           CVLNSACHEYL  D+P+ LQMVGES+ EWM +DNLE  KP+YSDFPASES+EG+  P  +
Sbjct: 301 CVLNSACHEYLEDDEPKTLQMVGESSNEWMYEDNLEEHKPHYSDFPASESLEGQCTPGYI 360

Query: 361 SNDSMSEAISLTEEQCSGTYISQGKSITHEAICQNNCEDMSEEISPTEEQCTDTYISEGK 420
           SN SMSEAIS T+EQ SGTY      IT+E I QNN EDMSE I+PTEEQC DTYIS+  
Sbjct: 361 SNYSMSEAISSTKEQLSGTY------ITNEVIFQNNSEDMSEAIAPTEEQCCDTYISQCI 420

Query: 421 SLTHETICLNNGEVLNHLHGMTNLNSLQLPEMSFGAEVCLTENGYKDTLELDHDRKGIST 480
             TH+ ICLN            NLNSL++ E S  AEVCLTE  YKD L   H+ KG  T
Sbjct: 421 PFTHDVICLN------------NLNSLKVQETSPEAEVCLTEISYKDKLAFVHE-KGTPT 480

Query: 481 EATSDCDLRADHGESISTKSTTDCNLSPDHEKSISTSSTSDGNLSPDQHLISIGKCPAQE 540
           E+ S+C+LR                  PDH K IST+S SDGNLSPDQHLIS G+CPA E
Sbjct: 481 ESNSNCNLR------------------PDHGKRISTNSISDGNLSPDQHLISTGECPATE 540

Query: 541 IEPQISNFSDSERNTSPDFHLDDSMDKFNQFEEPKRHPTRLLSTRTTISPTSQERLSKAM 600
            +PQ+SN+ DSERNT PDFHLD S+DKF Q EEPKRHPTRLL  RT+ISPTSQ+RLSK M
Sbjct: 541 RQPQMSNYYDSERNTPPDFHLDGSLDKFYQTEEPKRHPTRLLLKRTSISPTSQKRLSKGM 600

Query: 601 KSMRLHDKECKTCGGKPYFKQANYKVGTAEECDQMKRVYSDIFHEQNIRKSKKRSLHSTS 660
           +SM+LHDKE KTC GKPYF Q  Y+ G+AEECDQMK V+SD +H+Q IRKSKKRSLHS S
Sbjct: 601 RSMQLHDKEYKTCSGKPYFNQIKYRDGSAEECDQMKIVHSDTYHKQKIRKSKKRSLHSAS 660

Query: 661 NTKVPHGRTRSTAVQSCSDNAIAFTQRQMQDIESIALKLTNQLKSMKAIVEDRLHVEGNK 720
            T VP    RSTAVQ+CSD+AIAFTQRQMQDIE +ALKLTNQL SMKAIV+DRLHVEGN+
Sbjct: 661 TTVVPQASMRSTAVQNCSDSAIAFTQRQMQDIECLALKLTNQLTSMKAIVDDRLHVEGNQ 720

Query: 721 ATGFKFNTDEVRTAISDATKAEASAKKWLSMMSRDCNRFCKIMKTTENGSTASP-SAIQK 780
           AT FKFNTDEVRTA++DATKAEA A+KWLS+MSRDC+RFCKIMKTTE+GS  S  +AIQK
Sbjct: 721 ATSFKFNTDEVRTAVADATKAEAQARKWLSIMSRDCSRFCKIMKTTEHGSNVSSLTAIQK 756

Query: 781 IKRKITFADEAGGKLCEVRLIEDHVESFVE 810
           +KRKITFADEAGGKLCEVRLIED +    E
Sbjct: 781 LKRKITFADEAGGKLCEVRLIEDGINGRFE 756

BLAST of MS016812 vs. TAIR 10
Match: AT3G56870.1 (unknown protein; Has 204 Blast hits to 201 proteins in 58 species: Archae - 0; Bacteria - 10; Metazoa - 72; Fungi - 8; Plants - 41; Viruses - 0; Other Eukaryotes - 73 (source: NCBI BLink). )

HSP 1 Score: 168.7 bits (426), Expect = 1.9e-41
Identity = 220/833 (26.41%), Postives = 356/833 (42.74%), Query Frame = 0

Query: 1   MELRS----YNHFHYIHAIKGGLMTKVLNINSRGKPAVVFKKLTDIYEFIDEKDQNSLPT 60
           +ELRS     +H HYI  IK G +  V+N++ R KP + F++L DIY   D +   S+P 
Sbjct: 2   VELRSSTHLNSHVHYIRTIKDGSIKIVMNMDGRRKPKLKFRQLVDIYNLEDSQGVESIPR 61

Query: 61  QLLRERLEENIPEGYKFKVETEDFYAERKLFKDEPTVSDSGSGGD-TDGQKSDVEVDSMT 120
                                               V D  +G D T G +S+ +    T
Sbjct: 62  -----------------------------------VVRDLDNGSDFTQGSESE-DFSMTT 121

Query: 121 IQQIMEGCKKRKSRQSKSVDSSKEKLRTCSKQELERSCLLSDEDDDSDLNVALSIWKSKL 180
           ++ I + CK+RK +     D++ E   T S  E+++  +  DE    D+   LS W +K 
Sbjct: 122 LEMIQKQCKERKRKLRNCRDTTTE---TFSNVEVKKEYVTQDE--GCDIEEPLSSWDTKF 181

Query: 181 SRRKKLKTKCNGSRISTSSQCSQITGNSDPINSDQDLLPSSADLPIPVDIKVETPETDVT 240
           S+R+K K +   ++  TS+        S P        PS   + +P+ +    P     
Sbjct: 182 SKRRKKKQERKKAKCGTST--------SSP--------PSVEKVDLPLVLFHVKP----- 241

Query: 241 EIQNTNYIIDDLSLLCDENVNSCLSSELSLLCDENVNLCLSSEPIGADELFLNRGSTTSN 300
           E+ + +Y + + ++ C E   S +++ L                   +E+ L+  S++  
Sbjct: 242 EVWDDSYSVSE-AMDCSEKSESPINTVL------------------VEEIMLD--SSSDM 301

Query: 301 KEAEYCVLNSACHEYLVGDDPEFLQMVG------ESNTEWMKKDNLEIQKPNYSDFPASE 360
           +   YC             +P F  +V       +++ E+   D    Q   YS   + E
Sbjct: 302 RLVPYC-----------SAEPNFPGVVAIEEAFEDASEEFSDADFQNKQIVLYSSV-SRE 361

Query: 361 SMEGRYAPRCLSNDSMSEAISLTEEQCSGTYISQGKSITHEAICQNNCEDMSEEISPTEE 420
            ME    P+    +++          C   +IS        A   + CE+  +E    +E
Sbjct: 362 EMELDVNPQHSEYENIG---------CVKNFIS--------AYTSSGCEEEDKE----DE 421

Query: 421 QCTDTYISEGKSLTH-ETICLNNGEVLNHLHGMTNLNSLQLPEMSFGAEVCLTENGYKDT 480
           +  D   +   S+T  E + +   E+L          ++  P        C  ++  +  
Sbjct: 422 ESNDIKANLDMSVTGLEIVKIEAPEIL----------AIDYPGCLSIINFCAEDS--EIV 481

Query: 481 LELDHDRKGISTEATSDCDLRADHGESISTKSTTDCNLSPDHEKSISTSSTSDGNLSPDQ 540
            E +   K    EAT    L             T+C  S D+ + +   STS      + 
Sbjct: 482 WETEDITKDNFPEATDILQL-------------TNCCNSLDNLQPVPEDSTSS---KEED 541

Query: 541 HLIS-IGKCPAQEIEPQISNFSDSERNTSPDFHLDDSMDKFNQFEEPKRHPTRLLSTRTT 600
           HL   + +    + E +  +   S+    PD     +     Q ++P   P  LLS R  
Sbjct: 542 HLTERLQQSLYSKHEDEAGDHKLSQLYKEPDEVQKVAETDSIQQQQPHHQPENLLSGRKA 601

Query: 601 ISPTSQERLSKAMKSMRLHDKECKTCGGKPYFKQAN-YKVGTAEECDQMKRVYSDIFHEQ 660
           +SPTSQE+L KAM+     +K  K   GK YF   N +++  A+  D + RV      +Q
Sbjct: 602 LSPTSQEKLRKAMEHPDSPEKRSKKSRGKLYFSSQNSHRILKAQGLDNIDRVEIIPSSKQ 661

Query: 661 NIRKSK--------KRSLH-----STSNTKVPHGRTRSTAVQSCSDNAIAFTQRQMQDIE 720
            I+K+         +R+ H     +T   K     T  T++Q CS  AIAF+Q QM+D +
Sbjct: 662 AIQKATNNTRQMKYQRATHKFPRRNTQAAKAQPFSTGGTSIQGCSQKAIAFSQGQMRDFQ 689

Query: 721 SIALKLTNQLKSMKAIVEDRLHVEGNKATGFKFNTDEVRTAISDATKAEASAKKWLSMMS 780
           ++A +LT +LKSM+ I +  L  E N +     N DEV+T I +A K E S KKWLS++ 
Sbjct: 722 NVAARLTKELKSMRQITKRCLQAESNTSNMSDCNLDEVKTVIGNAEKTEESCKKWLSIIE 689

Query: 781 RDCNRFCKIMKTTENGSTASPSAIQKIKRKITFADEAGGKLCEVRLIEDHVES 807
           RDCNRFCK+M      S A+ + + K K+KI FAD+AGG LC V++ E  +ES
Sbjct: 782 RDCNRFCKLMSMVREDSPATENIVHK-KKKIRFADDAGGDLCHVKVFEIDLES 689