MS016811 (gene) Bitter gourd (TR) v1

BLAST of MS016811 vs. NCBI nr
Match: XP_022144258.1 (uncharacterized membrane protein At1g75140-like [Momordica charantia])

HSP 1 Score: 1292.7 bits (3344), Expect = 0.0e+00
Identity = 650/653 (99.54%), Postives = 650/653 (99.54%), Query Frame = 0

Query: 1   MANPRKGKLLLIYFLFVFGVPHVSRVFVCSSSSFEQNQPESESESEVPFEIRQRDLLKNL 60
           MANPRKGKLLLIYFLFVFGVPHVSRVFVCSSSSFEQNQPESESESEVPFEIRQRDLLKNL
Sbjct: 1   MANPRKGKLLLIYFLFVFGVPHVSRVFVCSSSSFEQNQPESESESEVPFEIRQRDLLKNL 60

Query: 61  EELVRNLSDVVSKLEMRLSDIPTEVIRDKKPNTDASRRVVPEVVSFGGRKEDGLNDGGDG 120
           EELVRNLSDVVSKLEMRLSDIPTEVIRDKKPNTDASRRVVPEVVS GGRKEDGLNDGGDG
Sbjct: 61  EELVRNLSDVVSKLEMRLSDIPTEVIRDKKPNTDASRRVVPEVVSLGGRKEDGLNDGGDG 120

Query: 121 GLESKIRDGDRTRAVSVTKYSPFWSERFHFLSAVKLDSDATCINVLPFRDFEGHSKYVAV 180
           GLESKIRDGDRTRAVSVTKYSPFWSERFHFLSAVKLDSDATCINVLPFRDFEGHSKYV V
Sbjct: 121 GLESKIRDGDRTRAVSVTKYSPFWSERFHFLSAVKLDSDATCINVLPFRDFEGHSKYVVV 180

Query: 181 GDEKGRVYVFTRNGDVAVELRTKSESPITTMLSYMSIYKNETLLVTGHKNGAILMHRIWE 240
           GDEKGRVYVFTRNGDVAVELRTKSESPI TMLSYMSIYKNETLLVTGHKNGAILMHRIWE
Sbjct: 181 GDEKGRVYVFTRNGDVAVELRTKSESPIMTMLSYMSIYKNETLLVTGHKNGAILMHRIWE 240

Query: 241 RSNGEESHSLFMEHVVEFVATDGREDESPISILEVHHVGRTRYILSSDVRGKIKVFRENG 300
           RSNGEESHSLFMEHVVEFVATDGREDESPISILEVHHVGRTRYILSSDVRGKIKVFRENG
Sbjct: 241 RSNGEESHSLFMEHVVEFVATDGREDESPISILEVHHVGRTRYILSSDVRGKIKVFRENG 300

Query: 301 TVYGSVMPESKPIAFLKQRLLFLTESGAGSLDLRSMKLRESECEGLNYSLARNYVFDATE 360
           TVYGSVMPESKPIAFLKQRLLFLTESGAGSLDLRSMKLRESECEGLNYSLARNYVFDATE
Sbjct: 301 TVYGSVMPESKPIAFLKQRLLFLTESGAGSLDLRSMKLRESECEGLNYSLARNYVFDATE 360

Query: 361 RSKAYGFTSTGDLIHVLLLGDIMNFKCRVRSKRKFELDEPLVFQAIKGYLLVISNEKVHI 420
           RSKAYGFTSTGDLIHVLLLGDIMNFKCRVRSKRKFELDEPLVFQAIKGYLLVISNEKVHI
Sbjct: 361 RSKAYGFTSTGDLIHVLLLGDIMNFKCRVRSKRKFELDEPLVFQAIKGYLLVISNEKVHI 420

Query: 421 FNVSSQHYARVGAPRPLFSAAVDEIRSSFLNYQNLDLESEGKFVPLISSDREKLVVLGLG 480
           FNVSSQHYARVGAPRPLFSAAVDEIRSSFLNYQNLDLESEGKFVPLISSDREKLVVLGLG
Sbjct: 421 FNVSSQHYARVGAPRPLFSAAVDEIRSSFLNYQNLDLESEGKFVPLISSDREKLVVLGLG 480

Query: 481 GGYVGMYRSNLPIFKGEFNTMLWTSPVLFFILFLFGAWHFFAKKKEALTSWGPDDPFTAT 540
           GGYVGMYRSNLPIFKGEFNTMLWTSPVLFFILFLFGAWHFFAKKKEALTSWGPDDPFTAT
Sbjct: 481 GGYVGMYRSNLPIFKGEFNTMLWTSPVLFFILFLFGAWHFFAKKKEALTSWGPDDPFTAT 540

Query: 541 SPTTGAPLGTGTSERSSFIDTPSRSTDMMDLRGGSGLRGPPRRYGSPTGYPGGATNSFRP 600
           SPTTGAPLGTGTSERSSFIDTPSRSTDMMDLRGGSGLRGPPRRYGSPTGYPGGATNSFRP
Sbjct: 541 SPTTGAPLGTGTSERSSFIDTPSRSTDMMDLRGGSGLRGPPRRYGSPTGYPGGATNSFRP 600

Query: 601 PTTSDHNSRPAAVDPNYRAASELKFRGSPLEPPGFPKRREPLFANNQVVDESS 654
           PTTSDHNSRPAAVDPNYRAASELKFRGSPLEPPGFPKRREPLFANNQVVDESS
Sbjct: 601 PTTSDHNSRPAAVDPNYRAASELKFRGSPLEPPGFPKRREPLFANNQVVDESS 653

BLAST of MS016811 vs. NCBI nr
Match: XP_038885478.1 (uncharacterized membrane protein At1g75140-like [Benincasa hispida])

HSP 1 Score: 1130.5 bits (2923), Expect = 0.0e+00
Identity = 578/661 (87.44%), Postives = 606/661 (91.68%), Query Frame = 0

Query: 1   MANPRKGKLLLIYFLFVFGVPHVSRVFVCSSSSFEQNQPESESESEVPFEIRQRDLLKNL 60
           MAN RKGKLL IYFLFVF  PHVSRVFVCSSSSFE NQ    SE EVPFEI+Q +LLKNL
Sbjct: 1   MANLRKGKLLFIYFLFVFSFPHVSRVFVCSSSSFELNQ----SEIEVPFEIKQYNLLKNL 60

Query: 61  EELVRNLSDVVSKLEMRLSDIPTEVIRDKKPNTDASRRVVPEVVSFGG----RKEDGLND 120
           EELVRNLSDVVSKLE+RLSDIPT V +DK  NTDASRRVV EVV  GG     KEDGLND
Sbjct: 61  EELVRNLSDVVSKLELRLSDIPTAVHKDKL-NTDASRRVVSEVVGLGGVKRKGKEDGLND 120

Query: 121 GGDGGLESKIRDGDRTRAVSVTKYSPFWSERFHFLSAVKLDSDATCINVLPFRDFEGHSK 180
           GGDGGL++KI+DG R RAVSVTKYSPFWSERFHFLSAVKL+SDATCINVLP RDFEGHSK
Sbjct: 121 GGDGGLDNKIQDGGRMRAVSVTKYSPFWSERFHFLSAVKLESDATCINVLPIRDFEGHSK 180

Query: 181 YVAVGDEKGRVYVFTRNGDVAVELRTKSESPITTMLSYMSIYKNETLLVTGHKNGAILMH 240
           YVAVGDEKGR+YVF RNGDVA+EL T SESPIT MLSY SIYKNETLLVTGH NGAILMH
Sbjct: 181 YVAVGDEKGRIYVFVRNGDVAIELPTVSESPITAMLSYTSIYKNETLLVTGHNNGAILMH 240

Query: 241 RIWERSNGEESHSLFMEHVVEFVATDGREDESPISILEVHHVGRTRYILSSDVRGKIKVF 300
           RIWE  NGEE +S+FMEHVVEFVATD RED+SPISILEVHHVGRTRYILSSD RGKIKVF
Sbjct: 241 RIWEGLNGEELNSIFMEHVVEFVATDSREDDSPISILEVHHVGRTRYILSSDFRGKIKVF 300

Query: 301 RENGTVYGSVMPESKPIAFLKQRLLFLTESGAGSLDLRSMKLRESECEGLNYSLARNYVF 360
           +E+GTVYGSV   S+PIAFLKQRLLFLTESGAGSLDLRSMKLRE+ CEGLNYSLARNYVF
Sbjct: 301 KEDGTVYGSVTLMSRPIAFLKQRLLFLTESGAGSLDLRSMKLREAGCEGLNYSLARNYVF 360

Query: 361 DATERSKAYGFTSTGDLIHVLLLGDIMNFKCRVRSKRKFELDEPLVFQAIKGYLLVISNE 420
           DATERSKAYGFTS GDLIHVLLLGDIMNFKCRVRSKRKFELDEPLVFQAIKGYLLVISNE
Sbjct: 361 DATERSKAYGFTSDGDLIHVLLLGDIMNFKCRVRSKRKFELDEPLVFQAIKGYLLVISNE 420

Query: 421 KVHIFNVSSQHYARVGAPRPLFSAAVDEIRSSFLNYQNLDLESEGKFVPLISSDREKLVV 480
           KVH+FNVSSQHY RV APR LFSA +DEIRSSFLNYQNL+LESEGKF+PLISSD EKLVV
Sbjct: 421 KVHVFNVSSQHYVRVAAPRLLFSAGLDEIRSSFLNYQNLNLESEGKFIPLISSDHEKLVV 480

Query: 481 LGLGGGYVGMYRSNLPIFKGEFNTMLWTSPVLFFILFLFGAWHFFAKKKEALTSWGPDDP 540
           LGLGGGYVGMYRSNLPIFKGEFN+MLWTSPVLFFILFLFGAW FFAKKKEALTSWGPDDP
Sbjct: 481 LGLGGGYVGMYRSNLPIFKGEFNSMLWTSPVLFFILFLFGAWQFFAKKKEALTSWGPDDP 540

Query: 541 FTATSPTTGAPLGTGTSERSSFIDTPSRSTDMMDLRGGSGLRGPPRRYGSPTGYPGGATN 600
           FTATSPTTG PLGTG+SER+SFIDTPSRSTDMMDLRGG G+RGPPR YGSPTGYPGGAT+
Sbjct: 541 FTATSPTTGVPLGTGSSERASFIDTPSRSTDMMDLRGGGGIRGPPRHYGSPTGYPGGATS 600

Query: 601 SFRPPTTSDHNSRPAAVDPNYRAASELKFRGSPLEPPGFPKRREPLFANNQV----VDES 654
           SFRPPTTSDH+SRPAAVDP+YRAASELKFRGSPLEPPGFPKRREPLFANNQV    VDES
Sbjct: 601 SFRPPTTSDHSSRPAAVDPSYRAASELKFRGSPLEPPGFPKRREPLFANNQVVNQMVDES 656

BLAST of MS016811 vs. NCBI nr
Match: XP_022996718.1 (uncharacterized membrane protein At1g75140-like [Cucurbita maxima])

HSP 1 Score: 1129.0 bits (2919), Expect = 0.0e+00
Identity = 575/671 (85.69%), Postives = 610/671 (90.91%), Query Frame = 0

Query: 1   MANPRKGKLLLIYFLFVFGVPHVSRVFVCSSSSFEQNQPESESESEVPFEIRQRDLLKNL 60
           MANPRKGKLL IYFLFVFG+PHVSR FVC SSSFE NQ    SE+EVPFE RQ DLLKNL
Sbjct: 1   MANPRKGKLLFIYFLFVFGIPHVSRDFVCCSSSFELNQ----SETEVPFETRQYDLLKNL 60

Query: 61  EELVRNLSDVVSKLEMRLSDIPTEVIRDKKPNTDASRRVVPEVVSFGGRKEDGLNDGGDG 120
           EELVRNLSDVVSKLE+RLS+IPT V RD K NTDASRRVVPEVVS GG KEDGLNDGGDG
Sbjct: 61  EELVRNLSDVVSKLELRLSNIPTAVHRDVKLNTDASRRVVPEVVSLGGGKEDGLNDGGDG 120

Query: 121 GLESKIRDGDRTRAVSVTKYSPFWSERFHFLSAVKLDSDATCINVLPFRDFEGHSKYVAV 180
            L++KI+DG R RAVSVTKYSPFWSERFHFLSAVKL+SDAT INVLP RDFEGHSKYVAV
Sbjct: 121 ALDTKIQDGGRGRAVSVTKYSPFWSERFHFLSAVKLESDATSINVLPLRDFEGHSKYVAV 180

Query: 181 GDEKGRVYVFTRNGDVAVELRTKSESPITTMLSYMSIYKNETLLVTGHKNGAILMHRIWE 240
           GDE GR+YVF RNGDVAVELRT S SPIT  LSYMSIYKNETLLVTGH++GAI M RIWE
Sbjct: 181 GDETGRIYVFVRNGDVAVELRTMSGSPITATLSYMSIYKNETLLVTGHESGAISMLRIWE 240

Query: 241 RSNGEESHSLFMEHVVEFVATDGREDESPISILEVHHVGRTRYILSSDVRGKIKVFRENG 300
            SNGEES+S+FMEHV+EFV TDGRE++SPISILEVHHVGRTRYILSSDV+GKIKVFRE+G
Sbjct: 241 GSNGEESNSIFMEHVLEFVVTDGREEKSPISILEVHHVGRTRYILSSDVKGKIKVFREDG 300

Query: 301 TVYGSVMPESKPIAFLKQRLLFLTESGAGSLDLRSMKLRESECEGLNYSLARNYVFDATE 360
           TVYGSVMP S+PIAFLKQRLLFLTE+GAGSLDLRSMKLRESECEGLNYSLARNYVFDATE
Sbjct: 301 TVYGSVMPTSRPIAFLKQRLLFLTETGAGSLDLRSMKLRESECEGLNYSLARNYVFDATE 360

Query: 361 RSKAYGFTSTGDLIHVLLLGDIMNFKCRVRSKRKFELDEPLVFQAIKGYLLVISNEKVHI 420
           RSKAYGFTS GDLIHV+LLGDIMNFKCRVRSK+KFELDEPL FQA+KGYLLVI +EKVH+
Sbjct: 361 RSKAYGFTSDGDLIHVMLLGDIMNFKCRVRSKKKFELDEPLFFQAVKGYLLVIGSEKVHV 420

Query: 421 FNVSSQHYARVGAPRPLFSAAVDEIRSSFLNYQNLDLESEGKFVPLISSDREKLVVLGLG 480
           FNVSSQHY RVGAPR LFSA +DEIRSSFLN QNLDLES+GKF+PLISSDREKLVVLGLG
Sbjct: 421 FNVSSQHYVRVGAPRLLFSARLDEIRSSFLNNQNLDLESQGKFIPLISSDREKLVVLGLG 480

Query: 481 GGYVGMYRSNLPIFKGEFNTMLWTSPVLFFILFLFGAWHFFAKKKEALTSWGPDDPFTAT 540
           GGY+GMYRSNLPI+KGEFNTMLWTSPVLFFILFLFGAW FFAKKKEALTSWGPDDPFTAT
Sbjct: 481 GGYIGMYRSNLPIYKGEFNTMLWTSPVLFFILFLFGAWQFFAKKKEALTSWGPDDPFTAT 540

Query: 541 SPTTGAPLGTGTSE--------------RSSFIDTPSRSTDMMDLRGGSGLRGPPRRYGS 600
           SPTTGAPLGTG++E              R+SFIDTPSRSTDMMDLRGGSGLRGPPRRYGS
Sbjct: 541 SPTTGAPLGTGSTERTSFIDTPSRSTDMRTSFIDTPSRSTDMMDLRGGSGLRGPPRRYGS 600

Query: 601 PTGYPGGATNSFRPPTTSDHNSRPAAVDPNYRAASELKFRGSPLEPPGFPKRREPLFAN- 654
           PTGYPGG T+SFRP TTSDH+SR AAVDPNYRAASELKFRGSPLEPPGFPKRREPLFAN 
Sbjct: 601 PTGYPGGVTSSFRPATTSDHSSRQAAVDPNYRAASELKFRGSPLEPPGFPKRREPLFANN 660

BLAST of MS016811 vs. NCBI nr
Match: XP_023546241.1 (uncharacterized membrane protein At1g75140-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1124.8 bits (2908), Expect = 0.0e+00
Identity = 570/671 (84.95%), Postives = 611/671 (91.06%), Query Frame = 0

Query: 1   MANPRKGKLLLIYFLFVFGVPHVSRVFVCSSSSFEQNQPESESESEVPFEIRQRDLLKNL 60
           MANPRKGKLL IYFLFVFG+P+VSR FVC SSS E NQ    SE+EVPFE RQ D+LKNL
Sbjct: 1   MANPRKGKLLFIYFLFVFGIPYVSRDFVCCSSSLELNQ----SETEVPFETRQYDVLKNL 60

Query: 61  EELVRNLSDVVSKLEMRLSDIPTEVIRDKKPNTDASRRVVPEVVSFGGRKEDGLNDGGDG 120
           EELVRNLSDVVSKLE+RLS+IPT V RD K NTDASRRVVPEVVS GG K+DGLNDGGDG
Sbjct: 61  EELVRNLSDVVSKLELRLSNIPTAVHRDVKLNTDASRRVVPEVVSLGGGKQDGLNDGGDG 120

Query: 121 GLESKIRDGDRTRAVSVTKYSPFWSERFHFLSAVKLDSDATCINVLPFRDFEGHSKYVAV 180
            L+++I+DG R RAVSVTKYSPFWSERFHF+SAVKL+SDAT INVLP RDFEGHSKYVAV
Sbjct: 121 DLDTRIQDGGRGRAVSVTKYSPFWSERFHFMSAVKLESDATSINVLPLRDFEGHSKYVAV 180

Query: 181 GDEKGRVYVFTRNGDVAVELRTKSESPITTMLSYMSIYKNETLLVTGHKNGAILMHRIWE 240
           GDE GR+YVF RNGDVAVELRT S SPIT MLSYMSIYKNETLLVTGH++GAI MHRIWE
Sbjct: 181 GDETGRIYVFVRNGDVAVELRTMSGSPITAMLSYMSIYKNETLLVTGHESGAISMHRIWE 240

Query: 241 RSNGEESHSLFMEHVVEFVATDGREDESPISILEVHHVGRTRYILSSDVRGKIKVFRENG 300
            SNGEES+S+FMEHV+EFV TDGRE++SPISILEVHHVGRTRYILSSDV+GKIKVFRE+G
Sbjct: 241 GSNGEESNSIFMEHVLEFVVTDGREEKSPISILEVHHVGRTRYILSSDVKGKIKVFREDG 300

Query: 301 TVYGSVMPESKPIAFLKQRLLFLTESGAGSLDLRSMKLRESECEGLNYSLARNYVFDATE 360
           TVYGSVMP S+PIAFLKQRLLFLTE+GAGSLDLRSMKLRESECEGLNYSLARNYVFDATE
Sbjct: 301 TVYGSVMPTSRPIAFLKQRLLFLTETGAGSLDLRSMKLRESECEGLNYSLARNYVFDATE 360

Query: 361 RSKAYGFTSTGDLIHVLLLGDIMNFKCRVRSKRKFELDEPLVFQAIKGYLLVISNEKVHI 420
           RSKAYGFTS GDLIHV+LLGDIMNFKCRVRSK+KFELDEPL+FQA+KGYLLVI +EKVH+
Sbjct: 361 RSKAYGFTSDGDLIHVMLLGDIMNFKCRVRSKKKFELDEPLLFQAVKGYLLVIGSEKVHV 420

Query: 421 FNVSSQHYARVGAPRPLFSAAVDEIRSSFLNYQNLDLESEGKFVPLISSDREKLVVLGLG 480
           FNVSSQHY RVGAPR LFSA +DEIRSSFLN QN DLES+GKF+PLISSDREKLVVLGLG
Sbjct: 421 FNVSSQHYVRVGAPRLLFSARLDEIRSSFLNNQNSDLESQGKFIPLISSDREKLVVLGLG 480

Query: 481 GGYVGMYRSNLPIFKGEFNTMLWTSPVLFFILFLFGAWHFFAKKKEALTSWGPDDPFTAT 540
           GGY+GMYRSNLPI+KGEFNTMLWTSPVLFFILFLFGAW FFAKKKEALTSWGPDDPFTAT
Sbjct: 481 GGYIGMYRSNLPIYKGEFNTMLWTSPVLFFILFLFGAWQFFAKKKEALTSWGPDDPFTAT 540

Query: 541 SPTTGAPLGTGTSE--------------RSSFIDTPSRSTDMMDLRGGSGLRGPPRRYGS 600
           SPTTGAPLGTG++E              R+SFIDTPSRSTDMMDLRGGSGLRGPPRRYGS
Sbjct: 541 SPTTGAPLGTGSTERTSFIDTPSRSTDMRTSFIDTPSRSTDMMDLRGGSGLRGPPRRYGS 600

Query: 601 PTGYPGGATNSFRPPTTSDHNSRPAAVDPNYRAASELKFRGSPLEPPGFPKRREPLFAN- 654
           PTGYPGG T+SFRP TTSDH+SR AAVDPNYRAASELKFRGSPLEPPGFPKRREPLFAN 
Sbjct: 601 PTGYPGGVTSSFRPATTSDHSSRQAAVDPNYRAASELKFRGSPLEPPGFPKRREPLFANN 660

BLAST of MS016811 vs. NCBI nr
Match: XP_022962086.1 (uncharacterized membrane protein At1g75140-like [Cucurbita moschata])

HSP 1 Score: 1120.9 bits (2898), Expect = 0.0e+00
Identity = 569/671 (84.80%), Postives = 609/671 (90.76%), Query Frame = 0

Query: 1   MANPRKGKLLLIYFLFVFGVPHVSRVFVCSSSSFEQNQPESESESEVPFEIRQRDLLKNL 60
           MANP KGKLL IYFLFVFG+P+VSR FVC SSS E NQ    SE+EVP+E RQ D+LKNL
Sbjct: 1   MANPWKGKLLFIYFLFVFGIPYVSRDFVCCSSSLELNQ----SETEVPYETRQYDVLKNL 60

Query: 61  EELVRNLSDVVSKLEMRLSDIPTEVIRDKKPNTDASRRVVPEVVSFGGRKEDGLNDGGDG 120
           EELVRNLSDVVSKLE+RLS+IPT V RD K NTDASRRVVPEVVS GG KEDGLNDGG G
Sbjct: 61  EELVRNLSDVVSKLELRLSNIPTAVHRDVKLNTDASRRVVPEVVSLGGGKEDGLNDGGHG 120

Query: 121 GLESKIRDGDRTRAVSVTKYSPFWSERFHFLSAVKLDSDATCINVLPFRDFEGHSKYVAV 180
            L+++++DG R RAVSVTKYSPFWSERFHFLSAVKL+SDAT INVLP RDFEGHSKYVAV
Sbjct: 121 ALDTRVQDGGRGRAVSVTKYSPFWSERFHFLSAVKLESDATSINVLPLRDFEGHSKYVAV 180

Query: 181 GDEKGRVYVFTRNGDVAVELRTKSESPITTMLSYMSIYKNETLLVTGHKNGAILMHRIWE 240
           GDE GR+YVF RNGDVAVELRT S SPIT MLSYMSIYKNETLLVTGH++GAI MHRIWE
Sbjct: 181 GDETGRIYVFVRNGDVAVELRTVSGSPITAMLSYMSIYKNETLLVTGHESGAISMHRIWE 240

Query: 241 RSNGEESHSLFMEHVVEFVATDGREDESPISILEVHHVGRTRYILSSDVRGKIKVFRENG 300
            SNGEES+S+FMEHV+EFV TDGRE++SPISILEVHHVGRTRYILSSDV+GKIKVFRE+G
Sbjct: 241 GSNGEESNSIFMEHVLEFVVTDGREEKSPISILEVHHVGRTRYILSSDVKGKIKVFREDG 300

Query: 301 TVYGSVMPESKPIAFLKQRLLFLTESGAGSLDLRSMKLRESECEGLNYSLARNYVFDATE 360
           TVYGSVMP S+PIAFLKQRLLFLTE+GAGSLDLRSMKLRESECEGLNYSLARNYVFDATE
Sbjct: 301 TVYGSVMPTSRPIAFLKQRLLFLTETGAGSLDLRSMKLRESECEGLNYSLARNYVFDATE 360

Query: 361 RSKAYGFTSTGDLIHVLLLGDIMNFKCRVRSKRKFELDEPLVFQAIKGYLLVISNEKVHI 420
           RSKAYGFTS GDLIHV+LLGDIMNFKCRVRSK+KFELDEPL FQA+KGYLLVI +EKVH+
Sbjct: 361 RSKAYGFTSDGDLIHVMLLGDIMNFKCRVRSKKKFELDEPLFFQAVKGYLLVIGSEKVHV 420

Query: 421 FNVSSQHYARVGAPRPLFSAAVDEIRSSFLNYQNLDLESEGKFVPLISSDREKLVVLGLG 480
           FNVSSQHY RVGAPR LFSA +DEIRSSFLN QNLDLES+GKF+PLISSDREKLVVLGLG
Sbjct: 421 FNVSSQHYVRVGAPRLLFSARLDEIRSSFLNNQNLDLESQGKFIPLISSDREKLVVLGLG 480

Query: 481 GGYVGMYRSNLPIFKGEFNTMLWTSPVLFFILFLFGAWHFFAKKKEALTSWGPDDPFTAT 540
           GGY+GMYRSNLPI+KGEFNTMLWTSPVLFFILFLFGAW FFAKKKEALTSWGPDDPFTAT
Sbjct: 481 GGYIGMYRSNLPIYKGEFNTMLWTSPVLFFILFLFGAWQFFAKKKEALTSWGPDDPFTAT 540

Query: 541 SPTTGAPLGTGTSE--------------RSSFIDTPSRSTDMMDLRGGSGLRGPPRRYGS 600
           SPTTGAPLGTG++E              R+SFIDTPSRSTDMMDLRGGSGLRGPPRRYGS
Sbjct: 541 SPTTGAPLGTGSTERTSFIDTPSRSTDMRTSFIDTPSRSTDMMDLRGGSGLRGPPRRYGS 600

Query: 601 PTGYPGGATNSFRPPTTSDHNSRPAAVDPNYRAASELKFRGSPLEPPGFPKRREPLFAN- 654
           PTGYPGG T+SFRP TTSDH+SR AAVDPNYRAASELKFRGSPLEPPGFPKRREPLFAN 
Sbjct: 601 PTGYPGGVTSSFRPATTSDHSSRQAAVDPNYRAASELKFRGSPLEPPGFPKRREPLFANN 660

BLAST of MS016811 vs. ExPASy Swiss-Prot
Match: Q9FRK5 (Uncharacterized membrane protein At1g75140 OS=Arabidopsis thaliana OX=3702 GN=At1g75140 PE=1 SV=3)

HSP 1 Score: 647.5 bits (1669), Expect = 1.6e-184
Identity = 356/665 (53.53%), Postives = 457/665 (68.72%), Query Frame = 0

Query: 1   MANPRKGKLLLIYF----LFVFGVPHVSRVFVCSSSSFE-QNQPESESESEVPFEIRQRD 60
           MA+ + GK    +F    + +F  P  S V    S     +N   S          RQ  
Sbjct: 1   MADSQNGKSAFFFFFVSLILLFLSPSYSDVTASESDPIPYENSDASPGVVTSSESDRQGV 60

Query: 61  LLKNLEELVRNLSDVVSKLEMRLSDIPTEVIRDKKPNTDASRRVVPEVVSFGGRKEDGLN 120
            L  LEELVRNL+++V++L+ +LS+ P +V        + +R  + E             
Sbjct: 61  SLHRLEELVRNLTELVARLDAKLSETPFKV------KKEITRDEIEE------------- 120

Query: 121 DGGDGGLESKIRDGDRTRAVSVTKYSPFWSERFHFLSAVKLDSDATCINVLPFRDFEGHS 180
                          + +A SVTKYSPFWSERF F SAVKLDS+ATCINVLPFRD EG S
Sbjct: 121 ---------------KAKAFSVTKYSPFWSERFEFTSAVKLDSEATCINVLPFRDHEGLS 180

Query: 181 KYVAVGDEKGRVYVFTRNGDVAVELRTKSESPITTMLSYMSIYKNETLLVTGHKNGAILM 240
           KY AVGD  GRV+VF RNGDV VE  T  +SPIT M+SYMS+YKNE+ +VTGH++G IL+
Sbjct: 181 KYFAVGDSSGRVFVFLRNGDVLVEFFTTCDSPITAMVSYMSVYKNESFVVTGHQSGVILL 240

Query: 241 HRIWERSNGEESHSLFMEHVVEFVATDGREDESPISILEVHHVGRTRYILSSDVRGKIKV 300
           HR+ E S GE+ +S  ME+V +F   DG ED   +++LEVHHVGR RYIL++D+ GK+ V
Sbjct: 241 HRLREGSIGEDLNSAVMENVGKF---DGTEDGLQVTLLEVHHVGRVRYILATDLSGKLTV 300

Query: 301 FRENGTVYGSVMPESKPIAFLKQRLLFLTESGAGSLDLRSMKLRESECEGLNYSLARNYV 360
           F EN TVYGSV P S+P+ FLKQRLLFLTE+GAGSLDLRSMK+RESECEGLN+SLAR+YV
Sbjct: 301 FTENRTVYGSVSPTSRPLVFLKQRLLFLTETGAGSLDLRSMKIRESECEGLNHSLARSYV 360

Query: 361 FDATERSKAYGFTSTGDLIHVLLLGDIMNFKCRVRSKRKFELDEPLVFQAIKGYLLVISN 420
           FDA+ER+KAYGFTS G++IHVLLLGDIMNFKCRVRSK+K +++EP+  QAIKGYLL+++ 
Sbjct: 361 FDASERAKAYGFTSEGEIIHVLLLGDIMNFKCRVRSKKKVQMEEPVALQAIKGYLLIVNQ 420

Query: 421 EKVHIFNVSSQHYARVGAPRPLFSAAVDEIRSSFLNY----QNLDLESEGKFVPLISSDR 480
           EKV ++NVS+QHY R   PR LF AA+++IRS+FL++    +  D +   K  PLI+SDR
Sbjct: 421 EKVFVYNVSTQHYVRTTGPRLLFPAALEDIRSTFLSHRESTKTTDHQKLEKVTPLIASDR 480

Query: 481 EKLVVLGLGGGYVGMYRSNLPIFKGEFNTMLWTSPVLFFILFLFGAWHFFAKKKEALTSW 540
           EKL+V+GLG GYV  Y+S LPI K EFNTMLW+SPV FFILFLFGAWHFF+KKKE+LT+W
Sbjct: 481 EKLLVMGLGDGYVATYKSKLPISKAEFNTMLWSSPVFFFILFLFGAWHFFSKKKESLTAW 540

Query: 541 GPDDPFTATSPTTGAPLGTGTSERSSFIDTPSRSTDMMDLRGGSGLRGPPRRYGSPTGY- 600
           GPDDPF++T+ ++ +   T     S+F ++  R+ D MDLR         RRY SP+ Y 
Sbjct: 541 GPDDPFSSTTMSSSST--TTAQNSSAFSESTRRNDDHMDLR---------RRYVSPSRYP 600

Query: 601 PGGATNSFRPPTTSDHNSRPAAVDPNYR-AASELKFR-GSPLEPPGFPKRREPLFANNQV 654
           PG AT ++R   ++D +SR      NYR  A E+K+R GS L+  GF KRRE LF NN+ 
Sbjct: 601 PGAATGAYRSVGSNDPSSRAPVETTNYRTTAQEMKYRGGSGLDSGGFGKRRESLFGNNKA 617

BLAST of MS016811 vs. ExPASy TrEMBL
Match: A0A6J1CSU7 (uncharacterized membrane protein At1g75140-like OS=Momordica charantia OX=3673 GN=LOC111013986 PE=4 SV=1)

HSP 1 Score: 1292.7 bits (3344), Expect = 0.0e+00
Identity = 650/653 (99.54%), Postives = 650/653 (99.54%), Query Frame = 0

Query: 1   MANPRKGKLLLIYFLFVFGVPHVSRVFVCSSSSFEQNQPESESESEVPFEIRQRDLLKNL 60
           MANPRKGKLLLIYFLFVFGVPHVSRVFVCSSSSFEQNQPESESESEVPFEIRQRDLLKNL
Sbjct: 1   MANPRKGKLLLIYFLFVFGVPHVSRVFVCSSSSFEQNQPESESESEVPFEIRQRDLLKNL 60

Query: 61  EELVRNLSDVVSKLEMRLSDIPTEVIRDKKPNTDASRRVVPEVVSFGGRKEDGLNDGGDG 120
           EELVRNLSDVVSKLEMRLSDIPTEVIRDKKPNTDASRRVVPEVVS GGRKEDGLNDGGDG
Sbjct: 61  EELVRNLSDVVSKLEMRLSDIPTEVIRDKKPNTDASRRVVPEVVSLGGRKEDGLNDGGDG 120

Query: 121 GLESKIRDGDRTRAVSVTKYSPFWSERFHFLSAVKLDSDATCINVLPFRDFEGHSKYVAV 180
           GLESKIRDGDRTRAVSVTKYSPFWSERFHFLSAVKLDSDATCINVLPFRDFEGHSKYV V
Sbjct: 121 GLESKIRDGDRTRAVSVTKYSPFWSERFHFLSAVKLDSDATCINVLPFRDFEGHSKYVVV 180

Query: 181 GDEKGRVYVFTRNGDVAVELRTKSESPITTMLSYMSIYKNETLLVTGHKNGAILMHRIWE 240
           GDEKGRVYVFTRNGDVAVELRTKSESPI TMLSYMSIYKNETLLVTGHKNGAILMHRIWE
Sbjct: 181 GDEKGRVYVFTRNGDVAVELRTKSESPIMTMLSYMSIYKNETLLVTGHKNGAILMHRIWE 240

Query: 241 RSNGEESHSLFMEHVVEFVATDGREDESPISILEVHHVGRTRYILSSDVRGKIKVFRENG 300
           RSNGEESHSLFMEHVVEFVATDGREDESPISILEVHHVGRTRYILSSDVRGKIKVFRENG
Sbjct: 241 RSNGEESHSLFMEHVVEFVATDGREDESPISILEVHHVGRTRYILSSDVRGKIKVFRENG 300

Query: 301 TVYGSVMPESKPIAFLKQRLLFLTESGAGSLDLRSMKLRESECEGLNYSLARNYVFDATE 360
           TVYGSVMPESKPIAFLKQRLLFLTESGAGSLDLRSMKLRESECEGLNYSLARNYVFDATE
Sbjct: 301 TVYGSVMPESKPIAFLKQRLLFLTESGAGSLDLRSMKLRESECEGLNYSLARNYVFDATE 360

Query: 361 RSKAYGFTSTGDLIHVLLLGDIMNFKCRVRSKRKFELDEPLVFQAIKGYLLVISNEKVHI 420
           RSKAYGFTSTGDLIHVLLLGDIMNFKCRVRSKRKFELDEPLVFQAIKGYLLVISNEKVHI
Sbjct: 361 RSKAYGFTSTGDLIHVLLLGDIMNFKCRVRSKRKFELDEPLVFQAIKGYLLVISNEKVHI 420

Query: 421 FNVSSQHYARVGAPRPLFSAAVDEIRSSFLNYQNLDLESEGKFVPLISSDREKLVVLGLG 480
           FNVSSQHYARVGAPRPLFSAAVDEIRSSFLNYQNLDLESEGKFVPLISSDREKLVVLGLG
Sbjct: 421 FNVSSQHYARVGAPRPLFSAAVDEIRSSFLNYQNLDLESEGKFVPLISSDREKLVVLGLG 480

Query: 481 GGYVGMYRSNLPIFKGEFNTMLWTSPVLFFILFLFGAWHFFAKKKEALTSWGPDDPFTAT 540
           GGYVGMYRSNLPIFKGEFNTMLWTSPVLFFILFLFGAWHFFAKKKEALTSWGPDDPFTAT
Sbjct: 481 GGYVGMYRSNLPIFKGEFNTMLWTSPVLFFILFLFGAWHFFAKKKEALTSWGPDDPFTAT 540

Query: 541 SPTTGAPLGTGTSERSSFIDTPSRSTDMMDLRGGSGLRGPPRRYGSPTGYPGGATNSFRP 600
           SPTTGAPLGTGTSERSSFIDTPSRSTDMMDLRGGSGLRGPPRRYGSPTGYPGGATNSFRP
Sbjct: 541 SPTTGAPLGTGTSERSSFIDTPSRSTDMMDLRGGSGLRGPPRRYGSPTGYPGGATNSFRP 600

Query: 601 PTTSDHNSRPAAVDPNYRAASELKFRGSPLEPPGFPKRREPLFANNQVVDESS 654
           PTTSDHNSRPAAVDPNYRAASELKFRGSPLEPPGFPKRREPLFANNQVVDESS
Sbjct: 601 PTTSDHNSRPAAVDPNYRAASELKFRGSPLEPPGFPKRREPLFANNQVVDESS 653

BLAST of MS016811 vs. ExPASy TrEMBL
Match: A0A6J1KBU3 (uncharacterized membrane protein At1g75140-like OS=Cucurbita maxima OX=3661 GN=LOC111491879 PE=4 SV=1)

HSP 1 Score: 1129.0 bits (2919), Expect = 0.0e+00
Identity = 575/671 (85.69%), Postives = 610/671 (90.91%), Query Frame = 0

Query: 1   MANPRKGKLLLIYFLFVFGVPHVSRVFVCSSSSFEQNQPESESESEVPFEIRQRDLLKNL 60
           MANPRKGKLL IYFLFVFG+PHVSR FVC SSSFE NQ    SE+EVPFE RQ DLLKNL
Sbjct: 1   MANPRKGKLLFIYFLFVFGIPHVSRDFVCCSSSFELNQ----SETEVPFETRQYDLLKNL 60

Query: 61  EELVRNLSDVVSKLEMRLSDIPTEVIRDKKPNTDASRRVVPEVVSFGGRKEDGLNDGGDG 120
           EELVRNLSDVVSKLE+RLS+IPT V RD K NTDASRRVVPEVVS GG KEDGLNDGGDG
Sbjct: 61  EELVRNLSDVVSKLELRLSNIPTAVHRDVKLNTDASRRVVPEVVSLGGGKEDGLNDGGDG 120

Query: 121 GLESKIRDGDRTRAVSVTKYSPFWSERFHFLSAVKLDSDATCINVLPFRDFEGHSKYVAV 180
            L++KI+DG R RAVSVTKYSPFWSERFHFLSAVKL+SDAT INVLP RDFEGHSKYVAV
Sbjct: 121 ALDTKIQDGGRGRAVSVTKYSPFWSERFHFLSAVKLESDATSINVLPLRDFEGHSKYVAV 180

Query: 181 GDEKGRVYVFTRNGDVAVELRTKSESPITTMLSYMSIYKNETLLVTGHKNGAILMHRIWE 240
           GDE GR+YVF RNGDVAVELRT S SPIT  LSYMSIYKNETLLVTGH++GAI M RIWE
Sbjct: 181 GDETGRIYVFVRNGDVAVELRTMSGSPITATLSYMSIYKNETLLVTGHESGAISMLRIWE 240

Query: 241 RSNGEESHSLFMEHVVEFVATDGREDESPISILEVHHVGRTRYILSSDVRGKIKVFRENG 300
            SNGEES+S+FMEHV+EFV TDGRE++SPISILEVHHVGRTRYILSSDV+GKIKVFRE+G
Sbjct: 241 GSNGEESNSIFMEHVLEFVVTDGREEKSPISILEVHHVGRTRYILSSDVKGKIKVFREDG 300

Query: 301 TVYGSVMPESKPIAFLKQRLLFLTESGAGSLDLRSMKLRESECEGLNYSLARNYVFDATE 360
           TVYGSVMP S+PIAFLKQRLLFLTE+GAGSLDLRSMKLRESECEGLNYSLARNYVFDATE
Sbjct: 301 TVYGSVMPTSRPIAFLKQRLLFLTETGAGSLDLRSMKLRESECEGLNYSLARNYVFDATE 360

Query: 361 RSKAYGFTSTGDLIHVLLLGDIMNFKCRVRSKRKFELDEPLVFQAIKGYLLVISNEKVHI 420
           RSKAYGFTS GDLIHV+LLGDIMNFKCRVRSK+KFELDEPL FQA+KGYLLVI +EKVH+
Sbjct: 361 RSKAYGFTSDGDLIHVMLLGDIMNFKCRVRSKKKFELDEPLFFQAVKGYLLVIGSEKVHV 420

Query: 421 FNVSSQHYARVGAPRPLFSAAVDEIRSSFLNYQNLDLESEGKFVPLISSDREKLVVLGLG 480
           FNVSSQHY RVGAPR LFSA +DEIRSSFLN QNLDLES+GKF+PLISSDREKLVVLGLG
Sbjct: 421 FNVSSQHYVRVGAPRLLFSARLDEIRSSFLNNQNLDLESQGKFIPLISSDREKLVVLGLG 480

Query: 481 GGYVGMYRSNLPIFKGEFNTMLWTSPVLFFILFLFGAWHFFAKKKEALTSWGPDDPFTAT 540
           GGY+GMYRSNLPI+KGEFNTMLWTSPVLFFILFLFGAW FFAKKKEALTSWGPDDPFTAT
Sbjct: 481 GGYIGMYRSNLPIYKGEFNTMLWTSPVLFFILFLFGAWQFFAKKKEALTSWGPDDPFTAT 540

Query: 541 SPTTGAPLGTGTSE--------------RSSFIDTPSRSTDMMDLRGGSGLRGPPRRYGS 600
           SPTTGAPLGTG++E              R+SFIDTPSRSTDMMDLRGGSGLRGPPRRYGS
Sbjct: 541 SPTTGAPLGTGSTERTSFIDTPSRSTDMRTSFIDTPSRSTDMMDLRGGSGLRGPPRRYGS 600

Query: 601 PTGYPGGATNSFRPPTTSDHNSRPAAVDPNYRAASELKFRGSPLEPPGFPKRREPLFAN- 654
           PTGYPGG T+SFRP TTSDH+SR AAVDPNYRAASELKFRGSPLEPPGFPKRREPLFAN 
Sbjct: 601 PTGYPGGVTSSFRPATTSDHSSRQAAVDPNYRAASELKFRGSPLEPPGFPKRREPLFANN 660

BLAST of MS016811 vs. ExPASy TrEMBL
Match: A0A6J1HC37 (uncharacterized membrane protein At1g75140-like OS=Cucurbita moschata OX=3662 GN=LOC111462650 PE=4 SV=1)

HSP 1 Score: 1120.9 bits (2898), Expect = 0.0e+00
Identity = 569/671 (84.80%), Postives = 609/671 (90.76%), Query Frame = 0

Query: 1   MANPRKGKLLLIYFLFVFGVPHVSRVFVCSSSSFEQNQPESESESEVPFEIRQRDLLKNL 60
           MANP KGKLL IYFLFVFG+P+VSR FVC SSS E NQ    SE+EVP+E RQ D+LKNL
Sbjct: 1   MANPWKGKLLFIYFLFVFGIPYVSRDFVCCSSSLELNQ----SETEVPYETRQYDVLKNL 60

Query: 61  EELVRNLSDVVSKLEMRLSDIPTEVIRDKKPNTDASRRVVPEVVSFGGRKEDGLNDGGDG 120
           EELVRNLSDVVSKLE+RLS+IPT V RD K NTDASRRVVPEVVS GG KEDGLNDGG G
Sbjct: 61  EELVRNLSDVVSKLELRLSNIPTAVHRDVKLNTDASRRVVPEVVSLGGGKEDGLNDGGHG 120

Query: 121 GLESKIRDGDRTRAVSVTKYSPFWSERFHFLSAVKLDSDATCINVLPFRDFEGHSKYVAV 180
            L+++++DG R RAVSVTKYSPFWSERFHFLSAVKL+SDAT INVLP RDFEGHSKYVAV
Sbjct: 121 ALDTRVQDGGRGRAVSVTKYSPFWSERFHFLSAVKLESDATSINVLPLRDFEGHSKYVAV 180

Query: 181 GDEKGRVYVFTRNGDVAVELRTKSESPITTMLSYMSIYKNETLLVTGHKNGAILMHRIWE 240
           GDE GR+YVF RNGDVAVELRT S SPIT MLSYMSIYKNETLLVTGH++GAI MHRIWE
Sbjct: 181 GDETGRIYVFVRNGDVAVELRTVSGSPITAMLSYMSIYKNETLLVTGHESGAISMHRIWE 240

Query: 241 RSNGEESHSLFMEHVVEFVATDGREDESPISILEVHHVGRTRYILSSDVRGKIKVFRENG 300
            SNGEES+S+FMEHV+EFV TDGRE++SPISILEVHHVGRTRYILSSDV+GKIKVFRE+G
Sbjct: 241 GSNGEESNSIFMEHVLEFVVTDGREEKSPISILEVHHVGRTRYILSSDVKGKIKVFREDG 300

Query: 301 TVYGSVMPESKPIAFLKQRLLFLTESGAGSLDLRSMKLRESECEGLNYSLARNYVFDATE 360
           TVYGSVMP S+PIAFLKQRLLFLTE+GAGSLDLRSMKLRESECEGLNYSLARNYVFDATE
Sbjct: 301 TVYGSVMPTSRPIAFLKQRLLFLTETGAGSLDLRSMKLRESECEGLNYSLARNYVFDATE 360

Query: 361 RSKAYGFTSTGDLIHVLLLGDIMNFKCRVRSKRKFELDEPLVFQAIKGYLLVISNEKVHI 420
           RSKAYGFTS GDLIHV+LLGDIMNFKCRVRSK+KFELDEPL FQA+KGYLLVI +EKVH+
Sbjct: 361 RSKAYGFTSDGDLIHVMLLGDIMNFKCRVRSKKKFELDEPLFFQAVKGYLLVIGSEKVHV 420

Query: 421 FNVSSQHYARVGAPRPLFSAAVDEIRSSFLNYQNLDLESEGKFVPLISSDREKLVVLGLG 480
           FNVSSQHY RVGAPR LFSA +DEIRSSFLN QNLDLES+GKF+PLISSDREKLVVLGLG
Sbjct: 421 FNVSSQHYVRVGAPRLLFSARLDEIRSSFLNNQNLDLESQGKFIPLISSDREKLVVLGLG 480

Query: 481 GGYVGMYRSNLPIFKGEFNTMLWTSPVLFFILFLFGAWHFFAKKKEALTSWGPDDPFTAT 540
           GGY+GMYRSNLPI+KGEFNTMLWTSPVLFFILFLFGAW FFAKKKEALTSWGPDDPFTAT
Sbjct: 481 GGYIGMYRSNLPIYKGEFNTMLWTSPVLFFILFLFGAWQFFAKKKEALTSWGPDDPFTAT 540

Query: 541 SPTTGAPLGTGTSE--------------RSSFIDTPSRSTDMMDLRGGSGLRGPPRRYGS 600
           SPTTGAPLGTG++E              R+SFIDTPSRSTDMMDLRGGSGLRGPPRRYGS
Sbjct: 541 SPTTGAPLGTGSTERTSFIDTPSRSTDMRTSFIDTPSRSTDMMDLRGGSGLRGPPRRYGS 600

Query: 601 PTGYPGGATNSFRPPTTSDHNSRPAAVDPNYRAASELKFRGSPLEPPGFPKRREPLFAN- 654
           PTGYPGG T+SFRP TTSDH+SR AAVDPNYRAASELKFRGSPLEPPGFPKRREPLFAN 
Sbjct: 601 PTGYPGGVTSSFRPATTSDHSSRQAAVDPNYRAASELKFRGSPLEPPGFPKRREPLFANN 660

BLAST of MS016811 vs. ExPASy TrEMBL
Match: A0A0A0LNK9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G361500 PE=4 SV=1)

HSP 1 Score: 1087.4 bits (2811), Expect = 0.0e+00
Identity = 550/652 (84.36%), Postives = 589/652 (90.34%), Query Frame = 0

Query: 1   MANPRKGKLLLIYFLFVFGVPHVSRVFVCSSSSFEQNQPESESESEVPFEIRQRDLLKNL 60
           MA  RKG+   I+FLF+F  PHVSRVFVCSSSS EQNQ    SE EVPFEI+Q DLLKNL
Sbjct: 1   MAIERKGRFFFIFFLFIFAFPHVSRVFVCSSSSLEQNQ----SEIEVPFEIKQYDLLKNL 60

Query: 61  EELVRNLSDVVSKLEMRLSDIPTEVIRDKKPNTDASRRVVPEVVSFGGR-KEDGLNDGGD 120
           EELVRNLSDVVSKLE+RLSDIPT V R+K  NTDA RRVVPEV     + KE+GLNDGGD
Sbjct: 61  EELVRNLSDVVSKLELRLSDIPTAVNREKL-NTDAFRRVVPEVGKEKRKGKEEGLNDGGD 120

Query: 121 GGLESKIRDGDRTRAVSVTKYSPFWSERFHFLSAVKLDSDATCINVLPFRDFEGHSKYVA 180
           GGL+ KI+DG RTRAVSVTKYS FWSERFHFLSAVKL++DATCINVLP RDFEGHSKYVA
Sbjct: 121 GGLDHKIQDGGRTRAVSVTKYSSFWSERFHFLSAVKLEADATCINVLPLRDFEGHSKYVA 180

Query: 181 VGDEKGRVYVFTRNGDVAVELRTKSESPITTMLSYMSIYKNETLLVTGHKNGAILMHRIW 240
           VGDE+GR+YVF  NGDVA+EL T   SP+T MLSYMSIYKNETLLVTGHKNGAILMHRIW
Sbjct: 181 VGDERGRIYVFVINGDVAIELPTVPGSPVTAMLSYMSIYKNETLLVTGHKNGAILMHRIW 240

Query: 241 ERSNGEESHSLFMEHVVEFVATDGREDESPISILEVHHVGRTRYILSSDVRGKIKVFREN 300
           E SNGE+ + +FMEHVVEFVAT+ REDES IS+LE+HHVGRTRYILSSD  GKIKVFRE+
Sbjct: 241 EGSNGEDLNLIFMEHVVEFVATNSREDESQISLLELHHVGRTRYILSSDFGGKIKVFRED 300

Query: 301 GTVYGSVMPESKPIAFLKQRLLFLTESGAGSLDLRSMKLRESECEGLNYSLARNYVFDAT 360
           GTVYGSVMP S+PIAFLKQRLLFLTESGAGSLDLRSMKLRESECEGLN+SLARNYVFDA 
Sbjct: 301 GTVYGSVMPTSRPIAFLKQRLLFLTESGAGSLDLRSMKLRESECEGLNHSLARNYVFDAM 360

Query: 361 ERSKAYGFTSTGDLIHVLLLGDIMNFKCRVRSKRKFELDEPLVFQAIKGYLLVISNEKVH 420
           ERSKAYG TS GDLIHVLLLGDIMNFKCRVRSKRKFELDEPL FQ IKGYLLV SNEKVH
Sbjct: 361 ERSKAYGVTSDGDLIHVLLLGDIMNFKCRVRSKRKFELDEPLAFQTIKGYLLVTSNEKVH 420

Query: 421 IFNVSSQHYARVGAPRPLFSAAVDEIRSSFLNYQNLDLESEGKFVPLISSDREKLVVLGL 480
           +FNVSSQHY RVGAPR LFSA +DEI+SSFLNYQN DL SE  F+PLISSD EKLVVLGL
Sbjct: 421 VFNVSSQHYVRVGAPRLLFSAGLDEIKSSFLNYQNSDLVSEANFIPLISSDHEKLVVLGL 480

Query: 481 GGGYVGMYRSNLPIFKGEFNTMLWTSPVLFFILFLFGAWHFFAKKKEALTSWGPDDPFTA 540
           GGGYVGMYRSNLPIFKGEFNTM+WTSPVLFFILFLFGAWHFFAKKKEALTSWGPDDPFTA
Sbjct: 481 GGGYVGMYRSNLPIFKGEFNTMVWTSPVLFFILFLFGAWHFFAKKKEALTSWGPDDPFTA 540

Query: 541 TSPTTGAPLGTGTSERSSFIDTPSRSTDMMDLRGGSGLRGPPRRYGSPTGYPGGATNSFR 600
           TSPTTGAP+GTG+SER+SFIDTPSRSTD+MDLR   GLRGPPRRYGSPTGYP GAT+SFR
Sbjct: 541 TSPTTGAPMGTGSSERASFIDTPSRSTDVMDLRSAGGLRGPPRRYGSPTGYPAGATSSFR 600

Query: 601 PPTTSDHNSRPAAVDPNYRAASELKFRGSPLEPPGFPKRREPLFANNQVVDE 652
           P TT+DH+SRPAAVDPNYRAASELKFRGSPLEPPGFPKRREPLFANNQVV++
Sbjct: 601 PATTNDHSSRPAAVDPNYRAASELKFRGSPLEPPGFPKRREPLFANNQVVNQ 647

BLAST of MS016811 vs. ExPASy TrEMBL
Match: A0A6J1GHT9 (uncharacterized membrane protein At1g75140-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111454317 PE=4 SV=1)

HSP 1 Score: 1081.2 bits (2795), Expect = 0.0e+00
Identity = 560/658 (85.11%), Postives = 593/658 (90.12%), Query Frame = 0

Query: 1   MANPRKGKLLLIYFLFVFGVPHVSRVFVCSSSSFEQNQPESESESEVPFEIRQRDLLKNL 60
           MANPRKGKLL IYFLFVF +  VSRVFVC+SSS E NQP    ES+VP EIRQ  LLKNL
Sbjct: 1   MANPRKGKLLFIYFLFVFAIHRVSRVFVCNSSSLELNQP----ESDVPIEIRQHVLLKNL 60

Query: 61  EELVRNLSDVVSKLEMRLSDIPTEVIRDKKPNTDASRRVVPEVVSFGGRKEDGLNDGGDG 120
           EELV+NLSDVVSKLEMRLSDIPT      K NT        EV S GG K  GL+DGGDG
Sbjct: 61  EELVKNLSDVVSKLEMRLSDIPT------KLNT--------EVGSLGGGK--GLSDGGDG 120

Query: 121 GLESKIRDGDRTRAVSVTKYSPFWSERFHFLSAVKLDSDATCINVLPFRDFEGHSKYVAV 180
           GL++ I+ G   RAV+VTKYSPFWSE+FHFLSAVKLDSDATCINVLP RDFEGHSKYVAV
Sbjct: 121 GLDNGIQYGGGGRAVTVTKYSPFWSEKFHFLSAVKLDSDATCINVLPLRDFEGHSKYVAV 180

Query: 181 GDEKGRVYVFTRNGDVAVELRTKSESPITTMLSYMSIYKNETLLVTGHKNGAILMHRIWE 240
           GDE+GRVYVF RNGDVAVELRT S+SPIT+MLSYMS+YKNETLLVTGHKNGAILMHRIWE
Sbjct: 181 GDERGRVYVFMRNGDVAVELRTVSDSPITSMLSYMSVYKNETLLVTGHKNGAILMHRIWE 240

Query: 241 RSNGEESHSLFMEHVVEFVATDGREDESPISILEVHHVGRTRYILSSDVRGKIKVFRENG 300
           RSNGE+ +S+FMEHVVEFVA    EDESPISILEVH+VGR RYILSS+VRGKIKVFRE+G
Sbjct: 241 RSNGEDLNSVFMEHVVEFVA----EDESPISILEVHYVGRIRYILSSNVRGKIKVFREDG 300

Query: 301 TVYGSVMPESKPIAFLKQRLLFLTESGAGSLDLRSMKLRESECEGLNYSLARNYVFDATE 360
           TVYGSVMP S+PI FLKQRLLFLTESGAGSLDLRSMKLRESECEGLN+SLARNYVFDA E
Sbjct: 301 TVYGSVMPMSRPIVFLKQRLLFLTESGAGSLDLRSMKLRESECEGLNHSLARNYVFDAVE 360

Query: 361 RSKAYGFTSTGDLIHVLLLGDIMNFKCRVRSKRKFELDEPLVFQAIKGYLLVISNEKVHI 420
           RSKAYGFTS GDLIHVLLLGDI+NFKCRVRSKRKFELDEPL+FQAIKGYLLVISNEKVHI
Sbjct: 361 RSKAYGFTSDGDLIHVLLLGDILNFKCRVRSKRKFELDEPLIFQAIKGYLLVISNEKVHI 420

Query: 421 FNVSSQHYARVGAPRPLFSAAVDEIRSSFLNYQNLDLESEGKFVPLISSDREKLVVLGLG 480
           +NVSSQHY RVGAPR LFSA +DEIRSSFLNYQN+D ES GKF+PLISSD EKLVVLGLG
Sbjct: 421 YNVSSQHYVRVGAPRHLFSAGLDEIRSSFLNYQNMDSESGGKFIPLISSDHEKLVVLGLG 480

Query: 481 GGYVGMYRSNLPIFKGEFNTMLWTSPVLFFILFLFGAWHFFAKKKEALTSWGPDDPFTAT 540
           GGYVG+YRSNLPIFKGEFNT+LWTSPVLFFILFLFGAWHFFAKKKEALTSWGPDDPFTAT
Sbjct: 481 GGYVGLYRSNLPIFKGEFNTVLWTSPVLFFILFLFGAWHFFAKKKEALTSWGPDDPFTAT 540

Query: 541 SPTTGAPLGTGTSERSSFIDTPSRSTDMMDLR-GGSGLRGPPRRYGSPTGYPGGATNSFR 600
           SPTTGAPLGTG+SER+SFID PSRSTDMMDLR GG GLRGPPRRYGSPTGYPGGAT+SFR
Sbjct: 541 SPTTGAPLGTGSSERASFIDAPSRSTDMMDLRGGGGGLRGPPRRYGSPTGYPGGATSSFR 600

Query: 601 PPTTSDHNSRPAAVDPNYRAASELKFRGSPLEPPGFPKRREPLFAN----NQVVDESS 654
           P TTSDHNSRPAAVDPNYRAASELKFRGSPLEPPGFPKRREPLFAN    NQVVDESS
Sbjct: 601 PATTSDHNSRPAAVDPNYRAASELKFRGSPLEPPGFPKRREPLFANNQVVNQVVDESS 634

BLAST of MS016811 vs. TAIR 10
Match: AT1G75140.1 (unknown protein; LOCATED IN: endoplasmic reticulum; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 13 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G19370.1); Has 51 Blast hits to 49 proteins in 18 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 46; Viruses - 0; Other Eukaryotes - 5 (source: NCBI BLink). )

HSP 1 Score: 647.5 bits (1669), Expect = 1.1e-185
Identity = 356/665 (53.53%), Postives = 457/665 (68.72%), Query Frame = 0

Query: 1   MANPRKGKLLLIYF----LFVFGVPHVSRVFVCSSSSFE-QNQPESESESEVPFEIRQRD 60
           MA+ + GK    +F    + +F  P  S V    S     +N   S          RQ  
Sbjct: 1   MADSQNGKSAFFFFFVSLILLFLSPSYSDVTASESDPIPYENSDASPGVVTSSESDRQGV 60

Query: 61  LLKNLEELVRNLSDVVSKLEMRLSDIPTEVIRDKKPNTDASRRVVPEVVSFGGRKEDGLN 120
            L  LEELVRNL+++V++L+ +LS+ P +V        + +R  + E             
Sbjct: 61  SLHRLEELVRNLTELVARLDAKLSETPFKV------KKEITRDEIEE------------- 120

Query: 121 DGGDGGLESKIRDGDRTRAVSVTKYSPFWSERFHFLSAVKLDSDATCINVLPFRDFEGHS 180
                          + +A SVTKYSPFWSERF F SAVKLDS+ATCINVLPFRD EG S
Sbjct: 121 ---------------KAKAFSVTKYSPFWSERFEFTSAVKLDSEATCINVLPFRDHEGLS 180

Query: 181 KYVAVGDEKGRVYVFTRNGDVAVELRTKSESPITTMLSYMSIYKNETLLVTGHKNGAILM 240
           KY AVGD  GRV+VF RNGDV VE  T  +SPIT M+SYMS+YKNE+ +VTGH++G IL+
Sbjct: 181 KYFAVGDSSGRVFVFLRNGDVLVEFFTTCDSPITAMVSYMSVYKNESFVVTGHQSGVILL 240

Query: 241 HRIWERSNGEESHSLFMEHVVEFVATDGREDESPISILEVHHVGRTRYILSSDVRGKIKV 300
           HR+ E S GE+ +S  ME+V +F   DG ED   +++LEVHHVGR RYIL++D+ GK+ V
Sbjct: 241 HRLREGSIGEDLNSAVMENVGKF---DGTEDGLQVTLLEVHHVGRVRYILATDLSGKLTV 300

Query: 301 FRENGTVYGSVMPESKPIAFLKQRLLFLTESGAGSLDLRSMKLRESECEGLNYSLARNYV 360
           F EN TVYGSV P S+P+ FLKQRLLFLTE+GAGSLDLRSMK+RESECEGLN+SLAR+YV
Sbjct: 301 FTENRTVYGSVSPTSRPLVFLKQRLLFLTETGAGSLDLRSMKIRESECEGLNHSLARSYV 360

Query: 361 FDATERSKAYGFTSTGDLIHVLLLGDIMNFKCRVRSKRKFELDEPLVFQAIKGYLLVISN 420
           FDA+ER+KAYGFTS G++IHVLLLGDIMNFKCRVRSK+K +++EP+  QAIKGYLL+++ 
Sbjct: 361 FDASERAKAYGFTSEGEIIHVLLLGDIMNFKCRVRSKKKVQMEEPVALQAIKGYLLIVNQ 420

Query: 421 EKVHIFNVSSQHYARVGAPRPLFSAAVDEIRSSFLNY----QNLDLESEGKFVPLISSDR 480
           EKV ++NVS+QHY R   PR LF AA+++IRS+FL++    +  D +   K  PLI+SDR
Sbjct: 421 EKVFVYNVSTQHYVRTTGPRLLFPAALEDIRSTFLSHRESTKTTDHQKLEKVTPLIASDR 480

Query: 481 EKLVVLGLGGGYVGMYRSNLPIFKGEFNTMLWTSPVLFFILFLFGAWHFFAKKKEALTSW 540
           EKL+V+GLG GYV  Y+S LPI K EFNTMLW+SPV FFILFLFGAWHFF+KKKE+LT+W
Sbjct: 481 EKLLVMGLGDGYVATYKSKLPISKAEFNTMLWSSPVFFFILFLFGAWHFFSKKKESLTAW 540

Query: 541 GPDDPFTATSPTTGAPLGTGTSERSSFIDTPSRSTDMMDLRGGSGLRGPPRRYGSPTGY- 600
           GPDDPF++T+ ++ +   T     S+F ++  R+ D MDLR         RRY SP+ Y 
Sbjct: 541 GPDDPFSSTTMSSSST--TTAQNSSAFSESTRRNDDHMDLR---------RRYVSPSRYP 600

Query: 601 PGGATNSFRPPTTSDHNSRPAAVDPNYR-AASELKFR-GSPLEPPGFPKRREPLFANNQV 654
           PG AT ++R   ++D +SR      NYR  A E+K+R GS L+  GF KRRE LF NN+ 
Sbjct: 601 PGAATGAYRSVGSNDPSSRAPVETTNYRTTAQEMKYRGGSGLDSGGFGKRRESLFGNNKA 617

BLAST of MS016811 vs. TAIR 10
Match: AT1G19370.1 (unknown protein; LOCATED IN: endoplasmic reticulum; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 13 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G75140.1); Has 45 Blast hits to 43 proteins in 15 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 44; Viruses - 0; Other Eukaryotes - 1 (source: NCBI BLink). )

HSP 1 Score: 633.3 bits (1632), Expect = 2.2e-181
Identity = 356/664 (53.61%), Postives = 449/664 (67.62%), Query Frame = 0

Query: 1   MANPRKGKLLLIYF--LFVFGVPHVSRVFVCSSSSFEQNQPE-SESESEVPFEIRQRDLL 60
           MA+P  GK   I F  LF F +  +S ++   S   E    + + S SE     R+  LL
Sbjct: 1   MADPLNGKSFFICFSLLFSFTLLFISPLYATESPVIEDVSTDVAVSVSETN---REAVLL 60

Query: 61  KNLEELVRNLSDVVSKLEMRLSDIPTEVIRDKKPNTDASRRVVPEVVSFGGRKEDGLNDG 120
            NLEELV+NL+++V+ L+ +LS  P +   +   + D            G  KE G    
Sbjct: 61  HNLEELVKNLTELVANLDAKLSATPLKEKNEISVDDD-----------IGEEKERG---- 120

Query: 121 GDGGLESKIRDGDRTRAVSVTKYSPFWSERFHFLSAVKLDSDATCINVLPFRDFEGHSKY 180
                        R +A SVTKYSPFWSERF F SAVKL+SDATCINVLPFRDFEG SKY
Sbjct: 121 -------------RAKAFSVTKYSPFWSERFQFTSAVKLNSDATCINVLPFRDFEGSSKY 180

Query: 181 VAVGDEKGRVYVFTRNGDVAVELRTKSESPITTMLSYMSIYKNETLLVTGHKNGAILMHR 240
            A+GD KGRVYVF RNGDV +E  T  +SP+T M+SY S++KN + +VTGH+NGA+L+HR
Sbjct: 181 FAIGDSKGRVYVFLRNGDVLIEFFTTVDSPVTAMVSYSSVFKNSSFVVTGHQNGAVLLHR 240

Query: 241 IWERSNGEE--SHSLFMEHVVEFVATDGREDESPISILEVHHVGRTRYILSSDVRGKIKV 300
           I E SNGE+  S+S+ MEHV +F   D  +   P+++LEVHHVGR RYIL++D+ GK+ V
Sbjct: 241 IHEGSNGEDWNSNSVSMEHVGKFDVDDSAD---PVTLLEVHHVGRVRYILATDLSGKLTV 300

Query: 301 FRENGTVYGSVMPESKPIAFLKQRLLFLTESGAGSLDLRSMKLRESECEGLNYSLARNYV 360
             EN TVYGSV+P S+P+ FLKQRLLFLTESGAGSLDLRSMK+RE+ECEGLN+SLAR YV
Sbjct: 301 LTENRTVYGSVIPSSRPLVFLKQRLLFLTESGAGSLDLRSMKIRETECEGLNHSLARTYV 360

Query: 361 FDATERSKAYGFTSTGDLIHVLLLGDIMNFKCRVRSKRKFELDEPLVFQAIKGYLLVISN 420
           FDA ERSKAYGFTS G++IHVLL GDIMNFKCRVRSK+KF+++EP+  Q+IKGYLLVI+ 
Sbjct: 361 FDAAERSKAYGFTSEGEIIHVLLHGDIMNFKCRVRSKKKFQMEEPVALQSIKGYLLVINE 420

Query: 421 EKVHIFNVSSQHYARVGAPRPLFSAAVDEIRSSFLNYQNLDLESEG--KFVPLISSDREK 480
           EKV  FNVS+QHY R   PR LFSA ++EIRS+FL+++     +    K  PLI+SDRE 
Sbjct: 421 EKVFAFNVSTQHYVRTAGPRLLFSAGLEEIRSAFLSHRESSSRTTTVVKTRPLIASDREN 480

Query: 481 LVVLGLGGGYVGMYRSNLPIFKGEFNTMLWTSPVLFFILFLFGAWHFFAKKKEALTSWGP 540
           L+V+GL  GY  +Y+S LP  KG+FNTMLW+SPV FFILFLFGAWHFFAKKKE+LT+WGP
Sbjct: 481 LLVIGLENGYFAVYKSKLPTLKGDFNTMLWSSPVFFFILFLFGAWHFFAKKKESLTAWGP 540

Query: 541 DDPFTATSPTTGAPLGTGTSERSSFIDTPSRSTDMMDLRGGSGLRGPPRRYGSPTGYPGG 600
           DDPFT     TGA     +++  +F +   R+ D+MDLR         RRY         
Sbjct: 541 DDPFT----PTGAQ--NSSAKEPTFTEPSRRNDDLMDLR---------RRY--------- 600

Query: 601 ATNSFRPPTTSDHNSRPAAVDPNYRAASE----LKFRGSPLEPPGFPKRREPLFANNQVV 654
           A  S+R    +D +SR A VD NYR  ++     +  GS L+  GF  RR+ LF NN+V+
Sbjct: 601 AGGSYRSVGANDPSSR-APVDGNYRTTAQDHNNYRGGGSGLDSNGFGNRRDHLFGNNKVL 605