MS016805 (gene) Bitter gourd (TR) v1

Overview
NameMS016805
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
Descriptionmethyltransferase-like protein 2 isoform X1
Locationscaffold9_1: 145360 .. 149710 (+)
RNA-Seq ExpressionMS016805
SyntenyMS016805
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGCATTGCTTAGCAAGGGATGAACTCTCTGGATTTATGGAATCTGGAATTTATCAGTTTGAGAATTCGAATTTCATATTCGTAGACCCAGTTCGTGTTCTGAATCGTTCGTACTCACGGTTTAGGGTTTCTCCTTCTGGTTACTACCAACGATTCTTTGATTTCGAAAGGAAGGGTTTCGGAAACGGGGACTGTTTATCTTCTGCAAAGAAGAGAAAGAGGAAGGAAAAGAAACCCCACTCTCTAAACGAGAAAGAACGTGCTGCCGATCAACGCCACCAGGTTTCCATGATTCTACTTTGGTCAGGGCTAACTTGTTTAATTTGATTTCACAAATGAAATCGTTGTTAAACTTTCTATCAAATGTTTAGACATATATTTTTTAAAATTATTCTGACAAGTATGAACCGGTTCTCGATGGCCAACTATCCCTTCTCTGCATTCTTGAAATGGCAATAGTGAATGATGTTCTCGTGCTGCAGCTGGCAAAGCCTTTGTTACTAAAGGCGCACGAATTGTTATTGCAAGCTACTGATCTGTTGGGAGTAATGAGCAGTTTAAACCGCGAGGTTGGTTCTTCAGTGGGAAGCAGATGGTTAGAATTTCCCAAAGACAATCAATCATTGATTGAACACGGGCAGGTTTGGCAAGCTCCCTTATATGAGATAACTCTTAAACGCCCTGAATCCGACCAGTCAATTGAAGTTGGAGGTTGGTCTTGTGATTCGGCAACTATTTGGAGGGGTTATTATATTTATCCTATTACATTTTCACAGTAGTTGGATTACACTAACTTTGTGTTAAGGAAATTTATTTTCTTCTGTTGCCCATATGTATGCTTGGCTACTCCATGCTGATAAGATGTAAGATTCTATAGTGCCTAAAGGAAGATATAAAATTCCTTGGAGGTTGGAGTTAGATAAGTTAGTTTATTGGTGGTGGTTTCAAGTTGCGACCAGGGGAAAATCTTGTCATCTCATACTTCATTTATGTTCAGTTCTTGATTAGTGGTTTCTTATGTCTCGGTTAATTTGGCTAAGTTCCTGCGATGTGTGAGTGTGACTGTTAAATTTTTAAAATTTTGTGGGCGTATAGTCTTAAGTTTTCTTAAAGGGGCAAAATTTTATATTCTAACTTCTTTTTTTTTTAATATAATATTTTTCATTCTAAAAATTGAAGGGAAAGTGTATATATGTATGTATGTATATATATATTCTTGGCTCTGCCTGCTTATTGGTTGTATGAATGTTGACAATTTATGTGCTTCCATTATGCTTCAGGATCCTCTTTAGAGCTTAGAGTAGCTCAATTATTTAATAACATGGTTGTCAATGAGACATGTGAGGAGGTGGAAGCTGAATTTCTTGATCGTCAGTATATATTGCCTCGTGAAAGTTGCTTCTATATGGTATGGTGTCACTTTGTTAACATCTTTCCATTTTTTATTTCAGGGGTCATTCATTAATGAAGAAACGAACTCAAGAAGTTTCATTTTCCCTTTGGCATCCTTGAATTAGAGTTATTTGTTATCCTTTATTTATTATTGCTGTTTAAGGAAGAAAAATCCTCCAACTATTTTCAGATGTCAAACAAGCATTGATTTGTACTTCTCATTTGGATATTTCAGATGCTTTCATTTCAAAATTTGTTAGGTATAGTTCTAAGTTTGTCTTACATGATATTATTTCTCTTGTTTCCATGTACAAGTCTGATTTGAGGCAGGTTCACAACCTTATTCCTGGTAAGGGCTGCATGTCTTGTTTCCATGTTTTATACTTTTAGTTTTACGTCTATGTTCAAAACGATTTAGAATTTCAAATATGCAAGGTTTCATTGACATTTCAGTTGATCTTTAGTTAAGTTACTGCTGCTACCAAATACCATTCTTAACATTACCTTAAGCAGTCAGACCTCCCGTCTCTAACAAGGTCTCTAACATGTGGTCTCTAGTTAATCCTACACCCCTTATTTTGTTAGATTGTTATTCAGGATTTGTGTAGAGAAATTGATTGTTAGGCTGAATGTTGGTTTAGAAAGAATAGTTTATTATATGCTTTGTTATCATGGTAAGTTTATATTTATTCTCGTCATCAGCAAAGTCTAATAACATTTTTGGTTGTGATGCAGCTACAGCTAGCCGTGGCTTCAGTCTTATTGTCGTTGATCCACCATGGGAAAACGGAAGTGCACAACAAAAATCAAAGTATGCTATTGTGTATTCATCTTATTCATTTCTAATAACCAAGCCTTTCTCCGATGGGACTGTAGATTTCCTATGCATTTAGGGTTAGTGTAAAGTACTTTCTGTTGGATAACTGGGATGGCTAATCGTACTTGAGGATAGTTTCGCCCCAAGGACAATCTCGTTGGCAAGGGCTTGAAGTATCTTGGTCATACCAGTTTAGAGGTATCAAGAACCTTCAGGTGATCTTATCACCAAAAATCTTTGGTATCTCCCAGTCTGGGCTTGGGCGGGCGTAGGTGCCCCTGGTTTAGGGGAGCAAAACTTGGACTCCTCGTTTAAAAAAAAAAAAACCAAAAATACCTTGGGAGATAGTTTCAGTTTGGTATCTCCCGAGTCTGGGCTTGGGCGGGCGTAGGTGCCCCTGGTTTAGGGGAGCAAAACTTGGACTCCTCGTTTAAAAAAAAAAAAAAACCAAAAATACCTTGGGAGATAGTTTCAGATGTATATCTGTATGAGGATGTTACCTTGTGTGTTTTTTTTGGAGGTTGTTTGCTTTAAGGGTTCACAAGGAAGGAGGGGAAGAAAGGAAAGAAAAAGACTGTTGGTACTTGGTATAAGAGAACATCTACCTACGTTGTCTTAGAATAATGGGGGATTTGTAGAAGTATATAAATTTAGTAGCACTTTAGCCAATAATAAACGTAGATTGAATAAATGAATTATTAAGATTTGGAAATATTTGATTTCATTTACCTATGTCAAGTTTAAACAAATCGATTATAGTTAAGTGATGTTGAAGTATCTTGCAACTGATTATGTTTACTGGGTGTTCTTGTTGATGGTCTCAAGTTAGCATTTGACTGAACCTATTGGGATAATGTCAATAGTTCGGAAAATTTTGATTGGAGCAATCAGTTTACGGTCTTTTCTCTATATGCTTGTTATTAGAATCTCATTGCGATCATTCATCCTTAGATGTTTGTGCTATACCTTCTTGCCACTATTGTTTAATCTGTTAGTACTTTCAGAAATTAACGCTTTCCTGTTTCATCAACATAGGTATCCTACGTTGCCCAATCGGTATTTCTTAGCTCTACCTATCAAGAAACTTTCTCATAGCAAAGGAGCACTGGTCGCCTTATGGGCGACTAACAGAGAAAAGTTCCATCGTTTTATTGCTATGGATCTGTTTCCTGCATGGGGAGTCAAACATATTTCCACTTATTACTGGTTGAAGGTCTATCTTTCTCTTCTCTGCCTCTCTCTCCTTGTTCTTCTGGCCTTCTTTATATCTTTTGAAATTACAGGTCAAAGGAGATGGTTCATTGATTGGTGATCTAGATCTCTTCCATCACAGGCCGTATGAATGCCTTCTCTTGGGCTATTGTTACGGTGAGGTATCGAGACATTGGCTGATGAAAAAAAAATCTTAATTTGATTAAGGGAAAAACTTACCTACCTACTGGAAGCATCTTTCTGTGTTGTGCCCACTAAGAGAAGAACAAAAAATAATTTGTATATATTTTGGTTAGATTATAAATTTATGATCATGTCTTTTTAATCATTAATATTTGAAAGTTTCAACTATGCCAATGAAAATTAGTGACTACATAAAAAATATAATCCAAAGTTTAATGGCGTAACGTAATTAAAGTTTTTAAGATTTAGGGACTGAATAGATACATTTGGTCAAAGTATTTGAACTAAATTTATAGGTTACCTGTATATTTCCAACTTAAATTACGGTGTTACCAGTTTTATCTTTTATGGGTGGAAAGCAGGTAAGTTTTTCCCAGTGGTTAGTGCCATTTAATGTTGATTGCTTTAAATTTCTTGTGAGTTAAGGATGTGGAAGACCTGAAAGTTTTGGAGCAAAGTGTACCTAAAGATCAAGTCATCATTAGCATTCCTGGTGATTACTCAAGGAAGCCACCAATTGGAGGTATCACTATCAACACTGCTTTCTTTGTTCTCACGTGGAATGAAATTCACCTTTATCTGTTCTACCGTCCCGCTTTGTACCCAGAGCTTCTCCAGAAGTATGTTCCGGGGCCGAAGCCAGGCCGGTGCATCGAACTGTTCGCTCGAGAGATGAATCCCGGATGGCTTTCTTGGGGGAACGAGCCCCTTCACTTCCAGGAATCCAAACACTTCACAAGGATA

mRNA sequence

ATGGAGCATTGCTTAGCAAGGGATGAACTCTCTGGATTTATGGAATCTGGAATTTATCAGTTTGAGAATTCGAATTTCATATTCGTAGACCCAGTTCGTGTTCTGAATCGTTCGTACTCACGGTTTAGGGTTTCTCCTTCTGGTTACTACCAACGATTCTTTGATTTCGAAAGGAAGGGTTTCGGAAACGGGGACTGTTTATCTTCTGCAAAGAAGAGAAAGAGGAAGGAAAAGAAACCCCACTCTCTAAACGAGAAAGAACGTGCTGCCGATCAACGCCACCAGCTGGCAAAGCCTTTGTTACTAAAGGCGCACGAATTGTTATTGCAAGCTACTGATCTGTTGGGAGTAATGAGCAGTTTAAACCGCGAGGTTGGTTCTTCAGTGGGAAGCAGATGGTTAGAATTTCCCAAAGACAATCAATCATTGATTGAACACGGGCAGGTTTGGCAAGCTCCCTTATATGAGATAACTCTTAAACGCCCTGAATCCGACCAGTCAATTGAAGTTGGAGGATCCTCTTTAGAGCTTAGAGTAGCTCAATTATTTAATAACATGGTTGTCAATGAGACATGTGAGGAGGTGGAAGCTGAATTTCTTGATCGTCAGTATATATTGCCTCGTGAAAGTTGCTTCTATATGTCTGATTTGAGGCAGGTTCACAACCTTATTCCTGCTACAGCTAGCCGTGGCTTCAGTCTTATTGTCGTTGATCCACCATGGGAAAACGGAAGTGCACAACAAAAATCAAAGTATCCTACGTTGCCCAATCGGTATTTCTTAGCTCTACCTATCAAGAAACTTTCTCATAGCAAAGGAGCACTGGTCGCCTTATGGGCGACTAACAGAGAAAAGTTCCATCGTTTTATTGCTATGGATCTGTTTCCTGCATGGGGAGTCAAACATATTTCCACTTATTACTGGTTGAAGGTCAAAGGAGATGGTTCATTGATTGGTGATCTAGATCTCTTCCATCACAGGCCGTATGAATGCCTTCTCTTGGGCTATTGTTACGGTGAGGATGTGGAAGACCTGAAAGTTTTGGAGCAAAGTGTACCTAAAGATCAAGTCATCATTAGCATTCCTGGTGATTACTCAAGGAAGCCACCAATTGGAGGTATCACTATCAACACTGCTTTCTTTGTTCTCACGTGGAATGAAATTCACCTTTATCTGTTCTACCGTCCCGCTTTGTACCCAGAGCTTCTCCAGAAGTATGTTCCGGGGCCGAAGCCAGGCCGGTGCATCGAACTGTTCGCTCGAGAGATGAATCCCGGATGGCTTTCTTGGGGGAACGAGCCCCTTCACTTCCAGGAATCCAAACACTTCACAAGGATA

Coding sequence (CDS)

ATGGAGCATTGCTTAGCAAGGGATGAACTCTCTGGATTTATGGAATCTGGAATTTATCAGTTTGAGAATTCGAATTTCATATTCGTAGACCCAGTTCGTGTTCTGAATCGTTCGTACTCACGGTTTAGGGTTTCTCCTTCTGGTTACTACCAACGATTCTTTGATTTCGAAAGGAAGGGTTTCGGAAACGGGGACTGTTTATCTTCTGCAAAGAAGAGAAAGAGGAAGGAAAAGAAACCCCACTCTCTAAACGAGAAAGAACGTGCTGCCGATCAACGCCACCAGCTGGCAAAGCCTTTGTTACTAAAGGCGCACGAATTGTTATTGCAAGCTACTGATCTGTTGGGAGTAATGAGCAGTTTAAACCGCGAGGTTGGTTCTTCAGTGGGAAGCAGATGGTTAGAATTTCCCAAAGACAATCAATCATTGATTGAACACGGGCAGGTTTGGCAAGCTCCCTTATATGAGATAACTCTTAAACGCCCTGAATCCGACCAGTCAATTGAAGTTGGAGGATCCTCTTTAGAGCTTAGAGTAGCTCAATTATTTAATAACATGGTTGTCAATGAGACATGTGAGGAGGTGGAAGCTGAATTTCTTGATCGTCAGTATATATTGCCTCGTGAAAGTTGCTTCTATATGTCTGATTTGAGGCAGGTTCACAACCTTATTCCTGCTACAGCTAGCCGTGGCTTCAGTCTTATTGTCGTTGATCCACCATGGGAAAACGGAAGTGCACAACAAAAATCAAAGTATCCTACGTTGCCCAATCGGTATTTCTTAGCTCTACCTATCAAGAAACTTTCTCATAGCAAAGGAGCACTGGTCGCCTTATGGGCGACTAACAGAGAAAAGTTCCATCGTTTTATTGCTATGGATCTGTTTCCTGCATGGGGAGTCAAACATATTTCCACTTATTACTGGTTGAAGGTCAAAGGAGATGGTTCATTGATTGGTGATCTAGATCTCTTCCATCACAGGCCGTATGAATGCCTTCTCTTGGGCTATTGTTACGGTGAGGATGTGGAAGACCTGAAAGTTTTGGAGCAAAGTGTACCTAAAGATCAAGTCATCATTAGCATTCCTGGTGATTACTCAAGGAAGCCACCAATTGGAGGTATCACTATCAACACTGCTTTCTTTGTTCTCACGTGGAATGAAATTCACCTTTATCTGTTCTACCGTCCCGCTTTGTACCCAGAGCTTCTCCAGAAGTATGTTCCGGGGCCGAAGCCAGGCCGGTGCATCGAACTGTTCGCTCGAGAGATGAATCCCGGATGGCTTTCTTGGGGGAACGAGCCCCTTCACTTCCAGGAATCCAAACACTTCACAAGGATA

Protein sequence

MEHCLARDELSGFMESGIYQFENSNFIFVDPVRVLNRSYSRFRVSPSGYYQRFFDFERKGFGNGDCLSSAKKRKRKEKKPHSLNEKERAADQRHQLAKPLLLKAHELLLQATDLLGVMSSLNREVGSSVGSRWLEFPKDNQSLIEHGQVWQAPLYEITLKRPESDQSIEVGGSSLELRVAQLFNNMVVNETCEEVEAEFLDRQYILPRESCFYMSDLRQVHNLIPATASRGFSLIVVDPPWENGSAQQKSKYPTLPNRYFLALPIKKLSHSKGALVALWATNREKFHRFIAMDLFPAWGVKHISTYYWLKVKGDGSLIGDLDLFHHRPYECLLLGYCYGEDVEDLKVLEQSVPKDQVIISIPGDYSRKPPIGGITINTAFFVLTWNEIHLYLFYRPALYPELLQKYVPGPKPGRCIELFAREMNPGWLSWGNEPLHFQESKHFTRI
Homology
BLAST of MS016805 vs. NCBI nr
Match: XP_022144198.1 (methyltransferase-like protein 2 isoform X2 [Momordica charantia])

HSP 1 Score: 818.9 bits (2114), Expect = 2.1e-233
Identity = 403/446 (90.36%), Postives = 408/446 (91.48%), Query Frame = 0

Query: 1   MEHCLARDELSGFMESGIYQFENSNFIFVDPVRVLNRSYSRFRVSPSGYYQRFFDFERKG 60
           MEHCLARDELSGFMESGIYQFENSNFIFVDPVRVLNRSYSRFRVSPSGYYQRFFDFERKG
Sbjct: 1   MEHCLARDELSGFMESGIYQFENSNFIFVDPVRVLNRSYSRFRVSPSGYYQRFFDFERKG 60

Query: 61  FGNGDCLSSAKKRKRKEKKPHSLNEKERAADQRHQLAKPLLLKAHELLLQATDLLGVMSS 120
           FGNGDCLSSAKKRKRKEKKPHSLNEKERAADQRHQLAKPLLLKAHELLLQATDLLGVMSS
Sbjct: 61  FGNGDCLSSAKKRKRKEKKPHSLNEKERAADQRHQLAKPLLLKAHELLLQATDLLGVMSS 120

Query: 121 LNREVGSSVGSRWLEFPKDNQSLIEHGQVWQAPLYEITLKRPESDQSIEVGGSSLELRVA 180
           LNR+VGSSVGSRWLEFPKDNQSLIEHGQVWQAPLYEITLKRPESDQSIEVG SS ELRVA
Sbjct: 121 LNRDVGSSVGSRWLEFPKDNQSLIEHGQVWQAPLYEITLKRPESDQSIEVGESSFELRVA 180

Query: 181 QLFNNMVVNETCEEVEAEFLDRQYILPRESCFYMSDLRQVHNLIPATASRGFSLIVVDPP 240
            LFNN+VVNET +EVEAEFLD QYILPRESCFYMSDLRQVHNLIPATAS GFSLIVVDPP
Sbjct: 181 SLFNNLVVNETRDEVEAEFLDHQYILPRESCFYMSDLRQVHNLIPATASCGFSLIVVDPP 240

Query: 241 WENGSAQQKSKYPTLPNRYFLALPIKKLSHSKGALVALWATNREKFHRFIAMDLFPAWGV 300
           WENGSAQQKSKYPTLPNRYFLALPIKKLSH KGALVALW TNREKFHRFI ++LFPAWGV
Sbjct: 241 WENGSAQQKSKYPTLPNRYFLALPIKKLSHRKGALVALWVTNREKFHRFIDVELFPAWGV 300

Query: 301 KHISTYYWLKVKGDGSLIGDLDLFHHRPYECLLLGYCYGEDVEDLKVLEQSVPKDQVIIS 360
           KHISTYYWLKVKGDGSLIGDLDLFHHRPYECLLLGYCYGEDVEDLKVLEQSVPKDQVIIS
Sbjct: 301 KHISTYYWLKVKGDGSLIGDLDLFHHRPYECLLLGYCYGEDVEDLKVLEQSVPKDQVIIS 360

Query: 361 IPGDYSRKPPIGGITINTAFFVLTWNEIHLYLFYRPALYPELLQKYVPGPKPGRCIELFA 420
           I GDYSRKPPIG                            ELLQKYVPGPKPGRCIELFA
Sbjct: 361 ITGDYSRKPPIG----------------------------ELLQKYVPGPKPGRCIELFA 418

Query: 421 REMNPGWLSWGNEPLHFQESKHFTRI 447
           REMNPGWLSWGNEPLHFQESKHFTRI
Sbjct: 421 REMNPGWLSWGNEPLHFQESKHFTRI 418

BLAST of MS016805 vs. NCBI nr
Match: XP_022144197.1 (methyltransferase-like protein 2 isoform X1 [Momordica charantia])

HSP 1 Score: 810.8 bits (2093), Expect = 5.7e-231
Identity = 403/456 (88.38%), Postives = 408/456 (89.47%), Query Frame = 0

Query: 1   MEHCLARDELSGFMESGIYQFENSNFIFVDPVRVLNRSYSRFRVSPSGYYQRFFDFERKG 60
           MEHCLARDELSGFMESGIYQFENSNFIFVDPVRVLNRSYSRFRVSPSGYYQRFFDFERKG
Sbjct: 1   MEHCLARDELSGFMESGIYQFENSNFIFVDPVRVLNRSYSRFRVSPSGYYQRFFDFERKG 60

Query: 61  FGNGDCLSSAKKRKRKEKKPHSLNEKERAADQRHQLAKPLLLKAHELLLQATDLLGVMSS 120
           FGNGDCLSSAKKRKRKEKKPHSLNEKERAADQRHQLAKPLLLKAHELLLQATDLLGVMSS
Sbjct: 61  FGNGDCLSSAKKRKRKEKKPHSLNEKERAADQRHQLAKPLLLKAHELLLQATDLLGVMSS 120

Query: 121 LNREVGSSVGSRWLEFPKDNQSLIEHGQVWQAPLYEITLKRPESDQSIEVGGSSLELRVA 180
           LNR+VGSSVGSRWLEFPKDNQSLIEHGQVWQAPLYEITLKRPESDQSIEVG SS ELRVA
Sbjct: 121 LNRDVGSSVGSRWLEFPKDNQSLIEHGQVWQAPLYEITLKRPESDQSIEVGESSFELRVA 180

Query: 181 QLFNNMVVNETCEEVEAEFLDRQYILPRESCFYM----------SDLRQVHNLIPATASR 240
            LFNN+VVNET +EVEAEFLD QYILPRESCFYM          SDLRQVHNLIPATAS 
Sbjct: 181 SLFNNLVVNETRDEVEAEFLDHQYILPRESCFYMDIISLVSMYKSDLRQVHNLIPATASC 240

Query: 241 GFSLIVVDPPWENGSAQQKSKYPTLPNRYFLALPIKKLSHSKGALVALWATNREKFHRFI 300
           GFSLIVVDPPWENGSAQQKSKYPTLPNRYFLALPIKKLSH KGALVALW TNREKFHRFI
Sbjct: 241 GFSLIVVDPPWENGSAQQKSKYPTLPNRYFLALPIKKLSHRKGALVALWVTNREKFHRFI 300

Query: 301 AMDLFPAWGVKHISTYYWLKVKGDGSLIGDLDLFHHRPYECLLLGYCYGEDVEDLKVLEQ 360
            ++LFPAWGVKHISTYYWLKVKGDGSLIGDLDLFHHRPYECLLLGYCYGEDVEDLKVLEQ
Sbjct: 301 DVELFPAWGVKHISTYYWLKVKGDGSLIGDLDLFHHRPYECLLLGYCYGEDVEDLKVLEQ 360

Query: 361 SVPKDQVIISIPGDYSRKPPIGGITINTAFFVLTWNEIHLYLFYRPALYPELLQKYVPGP 420
           SVPKDQVIISI GDYSRKPPIG                            ELLQKYVPGP
Sbjct: 361 SVPKDQVIISITGDYSRKPPIG----------------------------ELLQKYVPGP 420

Query: 421 KPGRCIELFAREMNPGWLSWGNEPLHFQESKHFTRI 447
           KPGRCIELFAREMNPGWLSWGNEPLHFQESKHFTRI
Sbjct: 421 KPGRCIELFAREMNPGWLSWGNEPLHFQESKHFTRI 428

BLAST of MS016805 vs. NCBI nr
Match: KAG7020528.1 (Methyltransferase-like protein 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 704.1 bits (1816), Expect = 7.5e-199
Identity = 346/446 (77.58%), Postives = 374/446 (83.86%), Query Frame = 0

Query: 1   MEHCLARDELSGFMESGIYQFENSNFIFVDPVRVLNRSYSRFRVSPSGYYQRFFDFERKG 60
           ME+ LARDELS F+ESGIY+F+NSNFIFVDPVRVLNRSYSRFRVSP+GYY+RFFD  +KG
Sbjct: 1   MENRLARDELSVFLESGIYRFQNSNFIFVDPVRVLNRSYSRFRVSPAGYYRRFFDHVKKG 60

Query: 61  FGNGDCLSSAKKRKRKEKKPHSLNEKERAADQRHQLAKPLLLKAHELLLQATDLLGVMSS 120
             + DCL +A+KRKRKEKK   LNEKE AADQRHQ+AKPLLL AHE LLQATDLL VM S
Sbjct: 61  LDDKDCLLNARKRKRKEKKSRCLNEKELAADQRHQVAKPLLLMAHESLLQATDLLSVMGS 120

Query: 121 LNREVGSSVGSRWLEFPKDNQSLIEHGQVWQAPLYEITLKRPESDQSIEVGGSSLELRVA 180
           LNRE+ SS GSRW+E P D+QSLIEHG VWQAPLYEITLK PES  S EV GSS + RV 
Sbjct: 121 LNREIDSSAGSRWVELPNDHQSLIEHGGVWQAPLYEITLKLPESSPSSEVAGSSFKNRVV 180

Query: 181 QLFNNMVVNETCEEVEAEFLDRQYILPRESCFYMSDLRQVHNLIPATASRGFSLIVVDPP 240
            LFNN+VVNET  EVEA+FLD QYILPRESCFYMSDLR VHNLIPATAS GFSLIV+DPP
Sbjct: 181 PLFNNLVVNETLNEVEAQFLDHQYILPRESCFYMSDLRMVHNLIPATASSGFSLIVIDPP 240

Query: 241 WENGSAQQKSKYPTLPNRYFLALPIKKLSHSKGALVALWATNREKFHRFIAMDLFPAWGV 300
           WENGSAQQKSKYPTLPNRYFLALPIKKLSHSKGALVALW TNREKFHRFI ++LFP+WGV
Sbjct: 241 WENGSAQQKSKYPTLPNRYFLALPIKKLSHSKGALVALWVTNREKFHRFIDLELFPSWGV 300

Query: 301 KHISTYYWLKVKGDGSLIGDLDLFHHRPYECLLLGYCYGE-DVEDLKVLEQSVPKDQVII 360
            HIST+YWLKVK D SLI DLDLFHHRPYECLLLGYC+GE D+EDLK+LEQ+VPKDQVII
Sbjct: 301 THISTFYWLKVKADASLISDLDLFHHRPYECLLLGYCHGEDDIEDLKILEQTVPKDQVII 360

Query: 361 SIPGDYSRKPPIGGITINTAFFVLTWNEIHLYLFYRPALYPELLQKYVPGPKPGRCIELF 420
           SIPGDYSRKPPIG                            ELLQ+YVPGP PGRCIELF
Sbjct: 361 SIPGDYSRKPPIG----------------------------ELLQEYVPGPTPGRCIELF 418

Query: 421 AREMNPGWLSWGNEPLHFQESKHFTR 446
           AREM PGWLSWGNEPLHFQES+HFTR
Sbjct: 421 AREMTPGWLSWGNEPLHFQESQHFTR 418

BLAST of MS016805 vs. NCBI nr
Match: XP_022951949.1 (methyltransferase-like protein 2 isoform X1 [Cucurbita moschata])

HSP 1 Score: 702.6 bits (1812), Expect = 2.2e-198
Identity = 345/446 (77.35%), Postives = 373/446 (83.63%), Query Frame = 0

Query: 1   MEHCLARDELSGFMESGIYQFENSNFIFVDPVRVLNRSYSRFRVSPSGYYQRFFDFERKG 60
           ME+ LARDELS F+ESGIY+F+NSNFIFVDPVRVLNRSYSRFRVSP+GYY+RFFD  +KG
Sbjct: 1   MENRLARDELSVFLESGIYRFQNSNFIFVDPVRVLNRSYSRFRVSPAGYYRRFFDHVKKG 60

Query: 61  FGNGDCLSSAKKRKRKEKKPHSLNEKERAADQRHQLAKPLLLKAHELLLQATDLLGVMSS 120
             + DCL +A+KRKRKEKK   LNEKE AADQRHQ+AKPLLL AHE LLQATDLL VM S
Sbjct: 61  LDDKDCLLNARKRKRKEKKSRCLNEKELAADQRHQVAKPLLLMAHESLLQATDLLSVMGS 120

Query: 121 LNREVGSSVGSRWLEFPKDNQSLIEHGQVWQAPLYEITLKRPESDQSIEVGGSSLELRVA 180
           LNRE+ SS GSRW+E P D+QSLIEHG VWQAPLYEITLK PES  S EV GSS + RV 
Sbjct: 121 LNREIDSSAGSRWVELPNDHQSLIEHGGVWQAPLYEITLKLPESSPSSEVAGSSFKNRVV 180

Query: 181 QLFNNMVVNETCEEVEAEFLDRQYILPRESCFYMSDLRQVHNLIPATASRGFSLIVVDPP 240
            LFNN+VVNET  EVEA+FLD QYILPRESCFYMSDLR VHNLIPATAS GFS IV+DPP
Sbjct: 181 PLFNNLVVNETLNEVEAQFLDHQYILPRESCFYMSDLRMVHNLIPATASSGFSFIVIDPP 240

Query: 241 WENGSAQQKSKYPTLPNRYFLALPIKKLSHSKGALVALWATNREKFHRFIAMDLFPAWGV 300
           WENGSAQQKSKYPTLPNRYFLALPIKKLSHSKGALVALW TNREKFHRFI ++LFP+WGV
Sbjct: 241 WENGSAQQKSKYPTLPNRYFLALPIKKLSHSKGALVALWVTNREKFHRFIDLELFPSWGV 300

Query: 301 KHISTYYWLKVKGDGSLIGDLDLFHHRPYECLLLGYCYGE-DVEDLKVLEQSVPKDQVII 360
            HIST+YWLKVK D SLI DLDLFHHRPYECLLLGYC+GE D+EDLK+LEQ+VPKDQVII
Sbjct: 301 THISTFYWLKVKADASLISDLDLFHHRPYECLLLGYCHGEDDIEDLKILEQTVPKDQVII 360

Query: 361 SIPGDYSRKPPIGGITINTAFFVLTWNEIHLYLFYRPALYPELLQKYVPGPKPGRCIELF 420
           SIPGDYSRKPPIG                            ELLQ+YVPGP PGRCIELF
Sbjct: 361 SIPGDYSRKPPIG----------------------------ELLQEYVPGPTPGRCIELF 418

Query: 421 AREMNPGWLSWGNEPLHFQESKHFTR 446
           AREM PGWLSWGNEPLHFQES+HFTR
Sbjct: 421 AREMTPGWLSWGNEPLHFQESQHFTR 418

BLAST of MS016805 vs. NCBI nr
Match: XP_023002716.1 (methyltransferase-like protein 2 isoform X1 [Cucurbita maxima])

HSP 1 Score: 701.8 bits (1810), Expect = 3.7e-198
Identity = 347/446 (77.80%), Postives = 371/446 (83.18%), Query Frame = 0

Query: 1   MEHCLARDELSGFMESGIYQFENSNFIFVDPVRVLNRSYSRFRVSPSGYYQRFFDFERKG 60
           ME  LARDELS F+ESGIY+F+NSNFIFVDPVRVLN SYSRFRVSPSGYY+RFFD  +KG
Sbjct: 1   MEDRLARDELSVFLESGIYRFQNSNFIFVDPVRVLNLSYSRFRVSPSGYYRRFFDHVKKG 60

Query: 61  FGNGDCLSSAKKRKRKEKKPHSLNEKERAADQRHQLAKPLLLKAHELLLQATDLLGVMSS 120
             N DCL +A+KRKRKEKK   LNEKE AADQRHQ+ KPLLLKAH  LLQATDLL VM S
Sbjct: 61  LDNRDCLLNARKRKRKEKKSRCLNEKELAADQRHQVTKPLLLKAHGSLLQATDLLSVMGS 120

Query: 121 LNREVGSSVGSRWLEFPKDNQSLIEHGQVWQAPLYEITLKRPESDQSIEVGGSSLELRVA 180
           LNRE+ SS GSRW+E P D+QSLIEHG VWQAPLYEITLK PES  SIEV GSS + RV 
Sbjct: 121 LNREIDSSAGSRWVELPNDHQSLIEHGGVWQAPLYEITLKLPESSPSIEVAGSSFKNRVV 180

Query: 181 QLFNNMVVNETCEEVEAEFLDRQYILPRESCFYMSDLRQVHNLIPATASRGFSLIVVDPP 240
            LFNN+VVNET  EVEAEFLD QYILPRESCFYMSDLR VHNLIPATAS GFSLIV+DPP
Sbjct: 181 PLFNNLVVNETLNEVEAEFLDHQYILPRESCFYMSDLRMVHNLIPATASSGFSLIVIDPP 240

Query: 241 WENGSAQQKSKYPTLPNRYFLALPIKKLSHSKGALVALWATNREKFHRFIAMDLFPAWGV 300
           WENGSAQQKSKYPTLPNRYFLALPIKKLSHSKGALVALW TNREKFHRFI ++LFP+WGV
Sbjct: 241 WENGSAQQKSKYPTLPNRYFLALPIKKLSHSKGALVALWVTNREKFHRFIELELFPSWGV 300

Query: 301 KHISTYYWLKVKGDGSLIGDLDLFHHRPYECLLLGYCYGE-DVEDLKVLEQSVPKDQVII 360
            HIST+YWLKVK D SLI DLDLFHHRPYECLLLGYC+GE D+EDLK+LEQ+VPKDQVII
Sbjct: 301 THISTFYWLKVKADASLISDLDLFHHRPYECLLLGYCHGEDDIEDLKILEQTVPKDQVII 360

Query: 361 SIPGDYSRKPPIGGITINTAFFVLTWNEIHLYLFYRPALYPELLQKYVPGPKPGRCIELF 420
           SIPGDYSRKPPIG                            ELLQ+YVPGP  GRCIELF
Sbjct: 361 SIPGDYSRKPPIG----------------------------ELLQEYVPGPTLGRCIELF 418

Query: 421 AREMNPGWLSWGNEPLHFQESKHFTR 446
           AREM PGWLSWGNEPLHFQES+HFTR
Sbjct: 421 AREMTPGWLSWGNEPLHFQESQHFTR 418

BLAST of MS016805 vs. ExPASy Swiss-Prot
Match: Q8LFA9 (Methyltransferase-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=At1g19340 PE=2 SV=2)

HSP 1 Score: 437.6 bits (1124), Expect = 1.7e-121
Identity = 229/447 (51.23%), Postives = 296/447 (66.22%), Query Frame = 0

Query: 8   DELSGFMESGIYQFENSNFIFVDPVRVLNRSYSRFRVSPSGYYQRFFDFERKGFGNGDCL 67
           D+L+ F++SGIY+ +  N+ F+D VR+ NRSY+RF+VSPS YY RFF+   K        
Sbjct: 5   DKLAQFLDSGIYESDEFNWFFLDTVRITNRSYTRFKVSPSAYYSRFFN--SKQLNQHSSE 64

Query: 68  SSAKKRKRKEKKP--HSLNEKERAADQRHQLAKPLLLKAHELLLQATDLLGVMSSLNREV 127
           S+ KKRKRK+K    H  +  E+A++ RHQ A+  L KAHE  L+  +LL +   L+ + 
Sbjct: 65  SNPKKRKRKQKNSSFHLPSVGEQASNLRHQEARLFLSKAHESFLKEIELLSLTKGLSDDN 124

Query: 128 GSSVGSRWLEFPKDNQSLIEHGQVWQAPLYEITLK-RPESDQSIEVGGSSLELRVAQLFN 187
                S   +   D  S IE G VWQAP YEITL      D     G S  E RV Q+FN
Sbjct: 125 DDDDSSLLNKCCDDEVSFIELGGVWQAPFYEITLSFNLHCDNE---GESCNEQRVFQVFN 184

Query: 188 NMVVNETCEEVEAEFLDRQYILPRESCFYMSDLRQVHNLIPATASRGFSLIVVDPPWENG 247
           N+VVNE  EEVEAEF +R+YI+PR SCFYMSDL  + NL+PA +  G++LIV+DPPWEN 
Sbjct: 185 NLVVNEIGEEVEAEFSNRRYIMPRNSCFYMSDLHHIRNLVPAKSEEGYNLIVIDPPWENA 244

Query: 248 SAQQKSKYPTLPNRYFLALPIKKLSHSKGALVALWATNREKFHRFIAMDLFPAWGVKHIS 307
           SA QKSKYPTLPN+YFL+LPIK+L+H++GALVALW TNREK   F+  +LFPAWG+K+++
Sbjct: 245 SAHQKSKYPTLPNQYFLSLPIKQLAHAEGALVALWVTNREKLLSFVEKELFPAWGIKYVA 304

Query: 308 TYYWLKVKGDGSLIGDLDLFHHRPYECLLLGYCY-----GEDVEDLKVLEQSVPKDQVII 367
           T YWLKVK DG+LI DLDL HH+PYE LLLGY +      E   D K+L+    K+Q+I+
Sbjct: 305 TMYWLKVKPDGTLICDLDLVHHKPYEYLLLGYHFTELAGSEKRSDFKLLD----KNQIIM 364

Query: 368 SIPGDYSRKPPIGGITINTAFFVLTWNEIHLYLFYRPALYPELLQKYVPGPKPGRCIELF 427
           SIPGD+SRKPPIG                            ++L K+ PG +P RC+ELF
Sbjct: 365 SIPGDFSRKPPIG----------------------------DILLKHTPGSQPARCLELF 414

Query: 428 AREMNPGWLSWGNEPLHFQESKHFTRI 447
           AREM  GW SWGNEPLHFQ+S++F ++
Sbjct: 425 AREMAAGWTSWGNEPLHFQDSRYFLKV 414

BLAST of MS016805 vs. ExPASy Swiss-Prot
Match: Q3U034 (N(6)-adenine-specific methyltransferase METTL4 OS=Mus musculus OX=10090 GN=Mettl4 PE=1 SV=1)

HSP 1 Score: 163.7 bits (413), Expect = 4.8e-39
Identity = 108/374 (28.88%), Postives = 181/374 (48.40%), Query Frame = 0

Query: 75  RKEKKPHSLNEKERAADQRHQLAKPLLLKAHELLLQATDLLGVMSSLNREVGSSVGSRWL 134
           +K K+  + N+ E  A + H   + L+L     L+Q     G +  L  +   S G   L
Sbjct: 131 KKRKRCIAFNQGELDAMEYHTKIRELILDGSSKLIQEGLRSGFLYPLVEKQDGSSGCITL 190

Query: 135 EFPKDNQSLIEHGQVWQAPLYEITLKRPESDQSIEVGGSSLELRVAQLFNNMVVNETCEE 194
                N S +         L E+ L      Q++++ G  + +    L + ++ N +   
Sbjct: 191 PLDACNLSELCEMAKHLPSLNEMEL------QTLQLMGDDVSVIELDLSSQIIENNSSFS 250

Query: 195 VEAEFLDRQYILPRESCFYMSDLRQVHNLIPATASRGFSLIVVDPPWENGSAQQKSKYPT 254
                + ++Y+LP +S F +SD+  +  L+     + F  IV+DPPWEN S ++ ++Y +
Sbjct: 251 KMITLMGQKYLLPPQSSFLLSDISCMQPLL--NCGKTFDAIVIDPPWENKSVKRSNRYSS 310

Query: 255 LPNRYFLALPIKKLSHSKGALVALWATNREKFHRFIAMDLFPAWGVKHISTYYWLKVKGD 314
           L  +    +PI KL+ +   L+  W TNR+K   F+  +L+P+W V+ ++ +YW+K+   
Sbjct: 311 LSPQQIKRMPIPKLA-AADCLIVTWVTNRQKHLCFVKEELYPSWSVEVVAEWYWVKITNS 370

Query: 315 GSLIGDLDLFHHRPYECLLLGYCYGEDVEDLK---VLEQSVPKDQVIISIPGD-YSRKPP 374
           G  +  LD  H +PYECL+LG    +    L+   V    VP  ++I+S+P   +S KPP
Sbjct: 371 GEFVFPLDSPHKKPYECLVLGRVKEKTPLALRNPDVRIPPVPDQKLIVSVPCVLHSHKPP 430

Query: 375 IGGITINTAFFVLTWNEIHLYLFYRPALYPELLQKYV-PGPKPGRCIELFAREMNPGWLS 434
           +                             E+L+ Y+ PG   G+C+ELFAR + PGW+S
Sbjct: 431 L----------------------------TEVLRDYIKPG---GQCLELFARNLQPGWMS 464

Query: 435 WGNEPLHFQESKHF 444
           WGNE L FQ   +F
Sbjct: 491 WGNEVLKFQHMDYF 464

BLAST of MS016805 vs. ExPASy Swiss-Prot
Match: Q8N3J2 (N(6)-adenine-specific methyltransferase METTL4 OS=Homo sapiens OX=9606 GN=METTL4 PE=1 SV=3)

HSP 1 Score: 150.6 bits (379), Expect = 4.2e-35
Identity = 108/381 (28.35%), Postives = 179/381 (46.98%), Query Frame = 0

Query: 75  RKEKKPHSLNEKERAADQRHQLAKPLLLKAHELLLQATDLLGVMSSLNREVGSSVGSRWL 134
           +K K+    N+ E  A + H   + L+L     L+Q     G +  L  +     GS+ +
Sbjct: 132 KKRKRCVVFNQGELDAMEYHTKIRELILDGSLQLIQEGLKSGFLYPLFEK--QDKGSKPI 191

Query: 135 EFPKDNQSLIEHGQVWQAPLYEITLKRPE----SDQSIEVGGSSLELRVAQLFNNMVVNE 194
             P D  SL E        L E+    P       Q++++      +    LF  +V N 
Sbjct: 192 TLPLDACSLSE--------LCEMAKHLPSLNEMEHQTLQLVEEDTSVTEQDLFLRVVENN 251

Query: 195 TCEEVEAEFLDRQYILPRESCFYMSDLRQVHNLIPATASRGFSLIVVDPPWENGSAQQKS 254
           +        + ++Y+LP +S F +SD+  +  L+     + F +IV+DPPW+N S ++ +
Sbjct: 252 SSFTKVITLMGQKYLLPPKSSFLLSDISCMQPLL--NYRKTFDVIVIDPPWQNKSVKRSN 311

Query: 255 KYPTLPNRYFLALPIKKLSHSKGALVALWATNREKFHRFIAMDLFPAWGVKHISTYYWLK 314
           +Y  L       +PI KL+ +   L+  W TNR+K  RFI  +L+P+W V+ ++ ++W+K
Sbjct: 312 RYSYLSPLQIQQIPIPKLA-APNCLLVTWVTNRQKHLRFIKEELYPSWSVEVVAEWHWVK 371

Query: 315 VKGDGSLIGDLDLFHHRPYECLLLGYCYGEDVEDLKVLEQSV---PKDQVIISIPGD-YS 374
           +   G  +  LD  H +PYE L+LG    +    L+  + +V   P  ++I+S+P   +S
Sbjct: 372 ITNSGEFVFPLDSPHKKPYEGLILGRVQEKTALPLRNADVNVLPIPDHKLIVSVPCTLHS 431

Query: 375 RKPPIGGITINTAFFVLTWNEIHLYLFYRPALYPELLQKYVPGPKP-GRCIELFAREMNP 434
            KPP+                             E+L+ Y+   KP G  +ELFAR + P
Sbjct: 432 HKPPLA----------------------------EVLKDYI---KPDGEYLELFARNLQP 468

Query: 435 GWLSWGNEPLHFQESKHFTRI 447
           GW SWGNE L FQ   +F  +
Sbjct: 492 GWTSWGNEVLKFQHVDYFIAV 468

BLAST of MS016805 vs. ExPASy Swiss-Prot
Match: Q09800 (Uncharacterized protein C22G7.07c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC22G7.07c PE=3 SV=2)

HSP 1 Score: 112.5 bits (280), Expect = 1.3e-23
Identity = 100/428 (23.36%), Postives = 174/428 (40.65%), Query Frame = 0

Query: 55  DFERKGFGNGDCLSSAKKRKRKEKKPHSLNEKERAADQRHQLAKPLLLKAHELLLQATDL 114
           D+E  G G    +  + +R R+  +  SL++ + + D+ H              +Q  D+
Sbjct: 47  DYENSGKGTVGQVLQSLQRVRRALQLRSLDDNQ-STDKSHH-------------VQFQDI 106

Query: 115 LGVMSSLNREVGSSVGSRWLEFPKDNQSLIEHGQVWQAPLYEITLKRPE---SDQSIE-- 174
           +           S+  +  + F  +N  L++    W + ++++ +K P     DQ ++  
Sbjct: 107 V---------YRSNTSASEIHFQAENAPLVQ----WYSDIFKVLVKAPVIQFEDQCLQWL 166

Query: 175 ---------------VGGSSLELRVAQLFNNMVVNE--------------TCEEVEAEFL 234
                              ++EL + +++   V N+                E  E    
Sbjct: 167 KMLNTIPPSYSFITIKSKDTVELGLEEIYQQCVQNDGASAIKLKLKGSKVITESSETNCR 226

Query: 235 DRQYILPRESCFYMSDLRQVHNLIPATASRGF---SLIVVDPPWENGSAQQKSKYPTLPN 294
           D  Y +P  S F M D+ +   ++             I++DPPW N S  + S Y    N
Sbjct: 227 DSIYYIPARSSFIMGDVEKTAQILLEAIDGHLEKPKCIIIDPPWPNKSVARSSVYKVNRN 286

Query: 295 -RYFLALPIKKLSHSKGALVALWATNREKFHRFIAMDLFPAWGVKHISTYYWLKVKGDGS 354
             Y  ALPI++ S SK  +VA+W TN+EK+  F+   LF  W +  +ST+ WLK+   G 
Sbjct: 287 LGYLKALPIQE-SLSKTGVVAVWCTNKEKYVNFVKKVLFKKWNLTLVSTWTWLKITAFGE 346

Query: 355 LIGDLDLFHHRPYECLLLGYCYGEDVEDLKVLEQSVPKDQVIISIPGDYSRKPPIGGITI 414
            + D+     +P+E LL+G       E   V    +P    II IP  +SRKP +     
Sbjct: 347 PLFDVYSNMRKPWEQLLIGV----TSEYTSVYSDKIPPTFTIIGIPDYHSRKPSLKP--- 406

Query: 415 NTAFFVLTWNEIHLYLFYRPALYPELLQKYVPGPKPGRCIELFAREMNPGWLSWGNEPLH 445
               F+  W             +     + +P       +E+F R + P W++WG EPL 
Sbjct: 407 ----FISRW-------------FNCSANESLP------VLEIFGRSLTPNWITWGREPLL 416

BLAST of MS016805 vs. ExPASy TrEMBL
Match: A0A6J1CSN8 (methyltransferase-like protein 2 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111013949 PE=3 SV=1)

HSP 1 Score: 818.9 bits (2114), Expect = 1.0e-233
Identity = 403/446 (90.36%), Postives = 408/446 (91.48%), Query Frame = 0

Query: 1   MEHCLARDELSGFMESGIYQFENSNFIFVDPVRVLNRSYSRFRVSPSGYYQRFFDFERKG 60
           MEHCLARDELSGFMESGIYQFENSNFIFVDPVRVLNRSYSRFRVSPSGYYQRFFDFERKG
Sbjct: 1   MEHCLARDELSGFMESGIYQFENSNFIFVDPVRVLNRSYSRFRVSPSGYYQRFFDFERKG 60

Query: 61  FGNGDCLSSAKKRKRKEKKPHSLNEKERAADQRHQLAKPLLLKAHELLLQATDLLGVMSS 120
           FGNGDCLSSAKKRKRKEKKPHSLNEKERAADQRHQLAKPLLLKAHELLLQATDLLGVMSS
Sbjct: 61  FGNGDCLSSAKKRKRKEKKPHSLNEKERAADQRHQLAKPLLLKAHELLLQATDLLGVMSS 120

Query: 121 LNREVGSSVGSRWLEFPKDNQSLIEHGQVWQAPLYEITLKRPESDQSIEVGGSSLELRVA 180
           LNR+VGSSVGSRWLEFPKDNQSLIEHGQVWQAPLYEITLKRPESDQSIEVG SS ELRVA
Sbjct: 121 LNRDVGSSVGSRWLEFPKDNQSLIEHGQVWQAPLYEITLKRPESDQSIEVGESSFELRVA 180

Query: 181 QLFNNMVVNETCEEVEAEFLDRQYILPRESCFYMSDLRQVHNLIPATASRGFSLIVVDPP 240
            LFNN+VVNET +EVEAEFLD QYILPRESCFYMSDLRQVHNLIPATAS GFSLIVVDPP
Sbjct: 181 SLFNNLVVNETRDEVEAEFLDHQYILPRESCFYMSDLRQVHNLIPATASCGFSLIVVDPP 240

Query: 241 WENGSAQQKSKYPTLPNRYFLALPIKKLSHSKGALVALWATNREKFHRFIAMDLFPAWGV 300
           WENGSAQQKSKYPTLPNRYFLALPIKKLSH KGALVALW TNREKFHRFI ++LFPAWGV
Sbjct: 241 WENGSAQQKSKYPTLPNRYFLALPIKKLSHRKGALVALWVTNREKFHRFIDVELFPAWGV 300

Query: 301 KHISTYYWLKVKGDGSLIGDLDLFHHRPYECLLLGYCYGEDVEDLKVLEQSVPKDQVIIS 360
           KHISTYYWLKVKGDGSLIGDLDLFHHRPYECLLLGYCYGEDVEDLKVLEQSVPKDQVIIS
Sbjct: 301 KHISTYYWLKVKGDGSLIGDLDLFHHRPYECLLLGYCYGEDVEDLKVLEQSVPKDQVIIS 360

Query: 361 IPGDYSRKPPIGGITINTAFFVLTWNEIHLYLFYRPALYPELLQKYVPGPKPGRCIELFA 420
           I GDYSRKPPIG                            ELLQKYVPGPKPGRCIELFA
Sbjct: 361 ITGDYSRKPPIG----------------------------ELLQKYVPGPKPGRCIELFA 418

Query: 421 REMNPGWLSWGNEPLHFQESKHFTRI 447
           REMNPGWLSWGNEPLHFQESKHFTRI
Sbjct: 421 REMNPGWLSWGNEPLHFQESKHFTRI 418

BLAST of MS016805 vs. ExPASy TrEMBL
Match: A0A6J1CT00 (methyltransferase-like protein 2 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111013949 PE=3 SV=1)

HSP 1 Score: 810.8 bits (2093), Expect = 2.8e-231
Identity = 403/456 (88.38%), Postives = 408/456 (89.47%), Query Frame = 0

Query: 1   MEHCLARDELSGFMESGIYQFENSNFIFVDPVRVLNRSYSRFRVSPSGYYQRFFDFERKG 60
           MEHCLARDELSGFMESGIYQFENSNFIFVDPVRVLNRSYSRFRVSPSGYYQRFFDFERKG
Sbjct: 1   MEHCLARDELSGFMESGIYQFENSNFIFVDPVRVLNRSYSRFRVSPSGYYQRFFDFERKG 60

Query: 61  FGNGDCLSSAKKRKRKEKKPHSLNEKERAADQRHQLAKPLLLKAHELLLQATDLLGVMSS 120
           FGNGDCLSSAKKRKRKEKKPHSLNEKERAADQRHQLAKPLLLKAHELLLQATDLLGVMSS
Sbjct: 61  FGNGDCLSSAKKRKRKEKKPHSLNEKERAADQRHQLAKPLLLKAHELLLQATDLLGVMSS 120

Query: 121 LNREVGSSVGSRWLEFPKDNQSLIEHGQVWQAPLYEITLKRPESDQSIEVGGSSLELRVA 180
           LNR+VGSSVGSRWLEFPKDNQSLIEHGQVWQAPLYEITLKRPESDQSIEVG SS ELRVA
Sbjct: 121 LNRDVGSSVGSRWLEFPKDNQSLIEHGQVWQAPLYEITLKRPESDQSIEVGESSFELRVA 180

Query: 181 QLFNNMVVNETCEEVEAEFLDRQYILPRESCFYM----------SDLRQVHNLIPATASR 240
            LFNN+VVNET +EVEAEFLD QYILPRESCFYM          SDLRQVHNLIPATAS 
Sbjct: 181 SLFNNLVVNETRDEVEAEFLDHQYILPRESCFYMDIISLVSMYKSDLRQVHNLIPATASC 240

Query: 241 GFSLIVVDPPWENGSAQQKSKYPTLPNRYFLALPIKKLSHSKGALVALWATNREKFHRFI 300
           GFSLIVVDPPWENGSAQQKSKYPTLPNRYFLALPIKKLSH KGALVALW TNREKFHRFI
Sbjct: 241 GFSLIVVDPPWENGSAQQKSKYPTLPNRYFLALPIKKLSHRKGALVALWVTNREKFHRFI 300

Query: 301 AMDLFPAWGVKHISTYYWLKVKGDGSLIGDLDLFHHRPYECLLLGYCYGEDVEDLKVLEQ 360
            ++LFPAWGVKHISTYYWLKVKGDGSLIGDLDLFHHRPYECLLLGYCYGEDVEDLKVLEQ
Sbjct: 301 DVELFPAWGVKHISTYYWLKVKGDGSLIGDLDLFHHRPYECLLLGYCYGEDVEDLKVLEQ 360

Query: 361 SVPKDQVIISIPGDYSRKPPIGGITINTAFFVLTWNEIHLYLFYRPALYPELLQKYVPGP 420
           SVPKDQVIISI GDYSRKPPIG                            ELLQKYVPGP
Sbjct: 361 SVPKDQVIISITGDYSRKPPIG----------------------------ELLQKYVPGP 420

Query: 421 KPGRCIELFAREMNPGWLSWGNEPLHFQESKHFTRI 447
           KPGRCIELFAREMNPGWLSWGNEPLHFQESKHFTRI
Sbjct: 421 KPGRCIELFAREMNPGWLSWGNEPLHFQESKHFTRI 428

BLAST of MS016805 vs. ExPASy TrEMBL
Match: A0A6J1GK89 (methyltransferase-like protein 2 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111454683 PE=3 SV=1)

HSP 1 Score: 702.6 bits (1812), Expect = 1.1e-198
Identity = 345/446 (77.35%), Postives = 373/446 (83.63%), Query Frame = 0

Query: 1   MEHCLARDELSGFMESGIYQFENSNFIFVDPVRVLNRSYSRFRVSPSGYYQRFFDFERKG 60
           ME+ LARDELS F+ESGIY+F+NSNFIFVDPVRVLNRSYSRFRVSP+GYY+RFFD  +KG
Sbjct: 1   MENRLARDELSVFLESGIYRFQNSNFIFVDPVRVLNRSYSRFRVSPAGYYRRFFDHVKKG 60

Query: 61  FGNGDCLSSAKKRKRKEKKPHSLNEKERAADQRHQLAKPLLLKAHELLLQATDLLGVMSS 120
             + DCL +A+KRKRKEKK   LNEKE AADQRHQ+AKPLLL AHE LLQATDLL VM S
Sbjct: 61  LDDKDCLLNARKRKRKEKKSRCLNEKELAADQRHQVAKPLLLMAHESLLQATDLLSVMGS 120

Query: 121 LNREVGSSVGSRWLEFPKDNQSLIEHGQVWQAPLYEITLKRPESDQSIEVGGSSLELRVA 180
           LNRE+ SS GSRW+E P D+QSLIEHG VWQAPLYEITLK PES  S EV GSS + RV 
Sbjct: 121 LNREIDSSAGSRWVELPNDHQSLIEHGGVWQAPLYEITLKLPESSPSSEVAGSSFKNRVV 180

Query: 181 QLFNNMVVNETCEEVEAEFLDRQYILPRESCFYMSDLRQVHNLIPATASRGFSLIVVDPP 240
            LFNN+VVNET  EVEA+FLD QYILPRESCFYMSDLR VHNLIPATAS GFS IV+DPP
Sbjct: 181 PLFNNLVVNETLNEVEAQFLDHQYILPRESCFYMSDLRMVHNLIPATASSGFSFIVIDPP 240

Query: 241 WENGSAQQKSKYPTLPNRYFLALPIKKLSHSKGALVALWATNREKFHRFIAMDLFPAWGV 300
           WENGSAQQKSKYPTLPNRYFLALPIKKLSHSKGALVALW TNREKFHRFI ++LFP+WGV
Sbjct: 241 WENGSAQQKSKYPTLPNRYFLALPIKKLSHSKGALVALWVTNREKFHRFIDLELFPSWGV 300

Query: 301 KHISTYYWLKVKGDGSLIGDLDLFHHRPYECLLLGYCYGE-DVEDLKVLEQSVPKDQVII 360
            HIST+YWLKVK D SLI DLDLFHHRPYECLLLGYC+GE D+EDLK+LEQ+VPKDQVII
Sbjct: 301 THISTFYWLKVKADASLISDLDLFHHRPYECLLLGYCHGEDDIEDLKILEQTVPKDQVII 360

Query: 361 SIPGDYSRKPPIGGITINTAFFVLTWNEIHLYLFYRPALYPELLQKYVPGPKPGRCIELF 420
           SIPGDYSRKPPIG                            ELLQ+YVPGP PGRCIELF
Sbjct: 361 SIPGDYSRKPPIG----------------------------ELLQEYVPGPTPGRCIELF 418

Query: 421 AREMNPGWLSWGNEPLHFQESKHFTR 446
           AREM PGWLSWGNEPLHFQES+HFTR
Sbjct: 421 AREMTPGWLSWGNEPLHFQESQHFTR 418

BLAST of MS016805 vs. ExPASy TrEMBL
Match: A0A6J1KM32 (methyltransferase-like protein 2 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111496492 PE=3 SV=1)

HSP 1 Score: 701.8 bits (1810), Expect = 1.8e-198
Identity = 347/446 (77.80%), Postives = 371/446 (83.18%), Query Frame = 0

Query: 1   MEHCLARDELSGFMESGIYQFENSNFIFVDPVRVLNRSYSRFRVSPSGYYQRFFDFERKG 60
           ME  LARDELS F+ESGIY+F+NSNFIFVDPVRVLN SYSRFRVSPSGYY+RFFD  +KG
Sbjct: 1   MEDRLARDELSVFLESGIYRFQNSNFIFVDPVRVLNLSYSRFRVSPSGYYRRFFDHVKKG 60

Query: 61  FGNGDCLSSAKKRKRKEKKPHSLNEKERAADQRHQLAKPLLLKAHELLLQATDLLGVMSS 120
             N DCL +A+KRKRKEKK   LNEKE AADQRHQ+ KPLLLKAH  LLQATDLL VM S
Sbjct: 61  LDNRDCLLNARKRKRKEKKSRCLNEKELAADQRHQVTKPLLLKAHGSLLQATDLLSVMGS 120

Query: 121 LNREVGSSVGSRWLEFPKDNQSLIEHGQVWQAPLYEITLKRPESDQSIEVGGSSLELRVA 180
           LNRE+ SS GSRW+E P D+QSLIEHG VWQAPLYEITLK PES  SIEV GSS + RV 
Sbjct: 121 LNREIDSSAGSRWVELPNDHQSLIEHGGVWQAPLYEITLKLPESSPSIEVAGSSFKNRVV 180

Query: 181 QLFNNMVVNETCEEVEAEFLDRQYILPRESCFYMSDLRQVHNLIPATASRGFSLIVVDPP 240
            LFNN+VVNET  EVEAEFLD QYILPRESCFYMSDLR VHNLIPATAS GFSLIV+DPP
Sbjct: 181 PLFNNLVVNETLNEVEAEFLDHQYILPRESCFYMSDLRMVHNLIPATASSGFSLIVIDPP 240

Query: 241 WENGSAQQKSKYPTLPNRYFLALPIKKLSHSKGALVALWATNREKFHRFIAMDLFPAWGV 300
           WENGSAQQKSKYPTLPNRYFLALPIKKLSHSKGALVALW TNREKFHRFI ++LFP+WGV
Sbjct: 241 WENGSAQQKSKYPTLPNRYFLALPIKKLSHSKGALVALWVTNREKFHRFIELELFPSWGV 300

Query: 301 KHISTYYWLKVKGDGSLIGDLDLFHHRPYECLLLGYCYGE-DVEDLKVLEQSVPKDQVII 360
            HIST+YWLKVK D SLI DLDLFHHRPYECLLLGYC+GE D+EDLK+LEQ+VPKDQVII
Sbjct: 301 THISTFYWLKVKADASLISDLDLFHHRPYECLLLGYCHGEDDIEDLKILEQTVPKDQVII 360

Query: 361 SIPGDYSRKPPIGGITINTAFFVLTWNEIHLYLFYRPALYPELLQKYVPGPKPGRCIELF 420
           SIPGDYSRKPPIG                            ELLQ+YVPGP  GRCIELF
Sbjct: 361 SIPGDYSRKPPIG----------------------------ELLQEYVPGPTLGRCIELF 418

Query: 421 AREMNPGWLSWGNEPLHFQESKHFTR 446
           AREM PGWLSWGNEPLHFQES+HFTR
Sbjct: 421 AREMTPGWLSWGNEPLHFQESQHFTR 418

BLAST of MS016805 vs. ExPASy TrEMBL
Match: A0A0A0LLE0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G361440 PE=3 SV=1)

HSP 1 Score: 696.8 bits (1797), Expect = 5.8e-197
Identity = 343/444 (77.25%), Postives = 371/444 (83.56%), Query Frame = 0

Query: 2   EHCLARDELSGFMESGIYQFENSNFIFVDPVRVLNRSYSRFRVSPSGYYQRFFDFERKGF 61
           + CLARDELS F+ESGIY+F +S FIFVDPVRVLNRSYSRFRVSP+GYY RFFD+ERKG 
Sbjct: 3   DRCLARDELSVFLESGIYRFVDSRFIFVDPVRVLNRSYSRFRVSPAGYYSRFFDYERKGL 62

Query: 62  GNGDCLSSAKKRKRKEKKPHSLNEKERAADQRHQLAKPLLLKAHELLLQATDLLGVMSSL 121
            N DCLS  KKRKRKEKK   LNEKE AADQRHQ+AKPLLLKA+ELLLQATDLL V  SL
Sbjct: 63  ENKDCLSYGKKRKRKEKKLRCLNEKECAADQRHQVAKPLLLKAYELLLQATDLLSVTRSL 122

Query: 122 NREVGSSVGSRWLEFPKDNQSLIEHGQVWQAPLYEITLKRPESDQSIEVGGSSLELRVAQ 181
           NRE GSS G      P D+Q LIEHG++WQAPLYEITLK PE DQS EVGGSS + RV  
Sbjct: 123 NRESGSSEGR---NLPSDHQLLIEHGRIWQAPLYEITLKLPEHDQSSEVGGSSFKDRVVP 182

Query: 182 LFNNMVVNETCEEVEAEFLDRQYILPRESCFYMSDLRQVHNLIPATASRGFSLIVVDPPW 241
           LFNN+VVNET +EVEAEFLD QYILPRESCFYMSDLRQVHNLIPA AS GFSLI++DPPW
Sbjct: 183 LFNNLVVNETSDEVEAEFLDHQYILPRESCFYMSDLRQVHNLIPAKASSGFSLILIDPPW 242

Query: 242 ENGSAQQKSKYPTLPNRYFLALPIKKLSHSKGALVALWATNREKFHRFIAMDLFPAWGVK 301
           ENGSAQQKSKYPTLPNRYFLALPIKKLSH KGALVALW TNREKFHRFI ++LFPAWGV 
Sbjct: 243 ENGSAQQKSKYPTLPNRYFLALPIKKLSHGKGALVALWVTNREKFHRFIDLELFPAWGVV 302

Query: 302 HISTYYWLKVKGDGSLIGDLDLFHHRPYECLLLGYCYGEDVEDLKVLEQSVPKDQVIISI 361
           HISTYYWLKVK DGSLI DLDLFHHRPYECLLLGYCYGEDV+D+K+L+Q++PKD+VIISI
Sbjct: 303 HISTYYWLKVKADGSLISDLDLFHHRPYECLLLGYCYGEDVQDVKILDQTLPKDRVIISI 362

Query: 362 PGDYSRKPPIGGITINTAFFVLTWNEIHLYLFYRPALYPELLQKYVPGPKPGRCIELFAR 421
           PGDYSRKPPI                             ELLQ+YVPGPKPGRCIELFAR
Sbjct: 363 PGDYSRKPPIA----------------------------ELLQEYVPGPKPGRCIELFAR 415

Query: 422 EMNPGWLSWGNEPLHFQESKHFTR 446
           EMNPGWLSWGNEPLHFQES++FTR
Sbjct: 423 EMNPGWLSWGNEPLHFQESQYFTR 415

BLAST of MS016805 vs. TAIR 10
Match: AT1G19340.1 (Methyltransferase MT-A70 family protein )

HSP 1 Score: 437.6 bits (1124), Expect = 1.2e-122
Identity = 229/447 (51.23%), Postives = 296/447 (66.22%), Query Frame = 0

Query: 8   DELSGFMESGIYQFENSNFIFVDPVRVLNRSYSRFRVSPSGYYQRFFDFERKGFGNGDCL 67
           D+L+ F++SGIY+ +  N+ F+D VR+ NRSY+RF+VSPS YY RFF+   K        
Sbjct: 5   DKLAQFLDSGIYESDEFNWFFLDTVRITNRSYTRFKVSPSAYYSRFFN--SKQLNQHSSE 64

Query: 68  SSAKKRKRKEKKP--HSLNEKERAADQRHQLAKPLLLKAHELLLQATDLLGVMSSLNREV 127
           S+ KKRKRK+K    H  +  E+A++ RHQ A+  L KAHE  L+  +LL +   L+ + 
Sbjct: 65  SNPKKRKRKQKNSSFHLPSVGEQASNLRHQEARLFLSKAHESFLKEIELLSLTKGLSDDN 124

Query: 128 GSSVGSRWLEFPKDNQSLIEHGQVWQAPLYEITLK-RPESDQSIEVGGSSLELRVAQLFN 187
                S   +   D  S IE G VWQAP YEITL      D     G S  E RV Q+FN
Sbjct: 125 DDDDSSLLNKCCDDEVSFIELGGVWQAPFYEITLSFNLHCDNE---GESCNEQRVFQVFN 184

Query: 188 NMVVNETCEEVEAEFLDRQYILPRESCFYMSDLRQVHNLIPATASRGFSLIVVDPPWENG 247
           N+VVNE  EEVEAEF +R+YI+PR SCFYMSDL  + NL+PA +  G++LIV+DPPWEN 
Sbjct: 185 NLVVNEIGEEVEAEFSNRRYIMPRNSCFYMSDLHHIRNLVPAKSEEGYNLIVIDPPWENA 244

Query: 248 SAQQKSKYPTLPNRYFLALPIKKLSHSKGALVALWATNREKFHRFIAMDLFPAWGVKHIS 307
           SA QKSKYPTLPN+YFL+LPIK+L+H++GALVALW TNREK   F+  +LFPAWG+K+++
Sbjct: 245 SAHQKSKYPTLPNQYFLSLPIKQLAHAEGALVALWVTNREKLLSFVEKELFPAWGIKYVA 304

Query: 308 TYYWLKVKGDGSLIGDLDLFHHRPYECLLLGYCY-----GEDVEDLKVLEQSVPKDQVII 367
           T YWLKVK DG+LI DLDL HH+PYE LLLGY +      E   D K+L+    K+Q+I+
Sbjct: 305 TMYWLKVKPDGTLICDLDLVHHKPYEYLLLGYHFTELAGSEKRSDFKLLD----KNQIIM 364

Query: 368 SIPGDYSRKPPIGGITINTAFFVLTWNEIHLYLFYRPALYPELLQKYVPGPKPGRCIELF 427
           SIPGD+SRKPPIG                            ++L K+ PG +P RC+ELF
Sbjct: 365 SIPGDFSRKPPIG----------------------------DILLKHTPGSQPARCLELF 414

Query: 428 AREMNPGWLSWGNEPLHFQESKHFTRI 447
           AREM  GW SWGNEPLHFQ+S++F ++
Sbjct: 425 AREMAAGWTSWGNEPLHFQDSRYFLKV 414

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022144198.12.1e-23390.36methyltransferase-like protein 2 isoform X2 [Momordica charantia][more]
XP_022144197.15.7e-23188.38methyltransferase-like protein 2 isoform X1 [Momordica charantia][more]
KAG7020528.17.5e-19977.58Methyltransferase-like protein 2 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022951949.12.2e-19877.35methyltransferase-like protein 2 isoform X1 [Cucurbita moschata][more]
XP_023002716.13.7e-19877.80methyltransferase-like protein 2 isoform X1 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q8LFA91.7e-12151.23Methyltransferase-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=At1g19340 PE... [more]
Q3U0344.8e-3928.88N(6)-adenine-specific methyltransferase METTL4 OS=Mus musculus OX=10090 GN=Mettl... [more]
Q8N3J24.2e-3528.35N(6)-adenine-specific methyltransferase METTL4 OS=Homo sapiens OX=9606 GN=METTL4... [more]
Q098001.3e-2323.36Uncharacterized protein C22G7.07c OS=Schizosaccharomyces pombe (strain 972 / ATC... [more]
Match NameE-valueIdentityDescription
A0A6J1CSN81.0e-23390.36methyltransferase-like protein 2 isoform X2 OS=Momordica charantia OX=3673 GN=LO... [more]
A0A6J1CT002.8e-23188.38methyltransferase-like protein 2 isoform X1 OS=Momordica charantia OX=3673 GN=LO... [more]
A0A6J1GK891.1e-19877.35methyltransferase-like protein 2 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC... [more]
A0A6J1KM321.8e-19877.80methyltransferase-like protein 2 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
A0A0A0LLE05.8e-19777.25Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G361440 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G19340.11.2e-12251.23Methyltransferase MT-A70 family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR007757MT-A70-likePFAMPF05063MT-A70coord: 232..370
e-value: 4.7E-21
score: 75.5
IPR007757MT-A70-likePROSITEPS51143MT_A70coord: 190..446
score: 22.03508
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 68..92
NoneNo IPR availablePANTHERPTHR12829N6-ADENOSINE-METHYLTRANSFERASEcoord: 13..444
NoneNo IPR availablePANTHERPTHR12829:SF4METHYLTRANSFERASE-LIKE PROTEIN 4coord: 13..444
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 212..362

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS016805.1MS016805.1mRNA