MS016797 (gene) Bitter gourd (TR) v1

Overview


Name	MS016797
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	RING-type domain-containing protein
Location	scaffold9_1: 86719 .. 87408 (-)
RNA-Seq Expression	MS016797
Synteny	MS016797

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGAGCGGGTTTGGGGAATCGACGAGCAGGCAACCCCAAAGCCCATCGTGTTCAGACAACAATAGTGATGCTAGTAATTTTGAGTGCAATATTTGCCTGGATTTAGCTCAAGATCCTATCGTAACTCTATGCGGTCATCTTTTCTGTTGGCCGTGCCTTTATAAATGGCTGCACATTCATTCCCATCCCCAGGAATGTCCAGTTTGCAAGGCCATCATAGAAGAGCAAAAGTTGGTTCCCTTATATGGTCGGGGAAAGACATCGACTGACCCGAGATCAAAAAGTATTCCTGGAATTAACATTCCTAATCGTCCTGCGGGACAGAGACCTGAAGCAGCAGGTCCTCCCCCAGAACCAAATCATTTCCCTCACAATGGGTTTGGATTCATGGGAGGTCTCGGAGGGTTTGCTCCCATGGCGTCTACAAGGTTTGGTAATTTCACCTTGTCTGCGGCATTTGGTGGTCTTGTCCCATCTTTATTCAACTTTCAGATGCATGGATATGCAGATCCAAGCATGTATGGTGCAGGTGCTGGCTTTCCATATGGGTTCCCTAACATGTTTCATGGTAGCCATGGTCACGCCCATGGATACTATCACCATCTCCGTCAAGGTCAGCAAGATTATTACCTGAAGCGGCTGCTTATGTTGATTGGTTTCTGTGTTCTTCTTGCCCTAATCTGGCAA

mRNA sequence

ATGGCGAGCGGGTTTGGGGAATCGACGAGCAGGCAACCCCAAAGCCCATCGTGTTCAGACAACAATAGTGATGCTAGTAATTTTGAGTGCAATATTTGCCTGGATTTAGCTCAAGATCCTATCGTAACTCTATGCGGTCATCTTTTCTGTTGGCCGTGCCTTTATAAATGGCTGCACATTCATTCCCATCCCCAGGAATGTCCAGTTTGCAAGGCCATCATAGAAGAGCAAAAGTTGGTTCCCTTATATGGTCGGGGAAAGACATCGACTGACCCGAGATCAAAAAGTATTCCTGGAATTAACATTCCTAATCGTCCTGCGGGACAGAGACCTGAAGCAGCAGGTCCTCCCCCAGAACCAAATCATTTCCCTCACAATGGGTTTGGATTCATGGGAGGTCTCGGAGGGTTTGCTCCCATGGCGTCTACAAGGTTTGGTAATTTCACCTTGTCTGCGGCATTTGGTGGTCTTGTCCCATCTTTATTCAACTTTCAGATGCATGGATATGCAGATCCAAGCATGTATGGTGCAGGTGCTGGCTTTCCATATGGGTTCCCTAACATGTTTCATGGTAGCCATGGTCACGCCCATGGATACTATCACCATCTCCGTCAAGGTCAGCAAGATTATTACCTGAAGCGGCTGCTTATGTTGATTGGTTTCTGTGTTCTTCTTGCCCTAATCTGGCAA

Coding sequence (CDS)

ATGGCGAGCGGGTTTGGGGAATCGACGAGCAGGCAACCCCAAAGCCCATCGTGTTCAGACAACAATAGTGATGCTAGTAATTTTGAGTGCAATATTTGCCTGGATTTAGCTCAAGATCCTATCGTAACTCTATGCGGTCATCTTTTCTGTTGGCCGTGCCTTTATAAATGGCTGCACATTCATTCCCATCCCCAGGAATGTCCAGTTTGCAAGGCCATCATAGAAGAGCAAAAGTTGGTTCCCTTATATGGTCGGGGAAAGACATCGACTGACCCGAGATCAAAAAGTATTCCTGGAATTAACATTCCTAATCGTCCTGCGGGACAGAGACCTGAAGCAGCAGGTCCTCCCCCAGAACCAAATCATTTCCCTCACAATGGGTTTGGATTCATGGGAGGTCTCGGAGGGTTTGCTCCCATGGCGTCTACAAGGTTTGGTAATTTCACCTTGTCTGCGGCATTTGGTGGTCTTGTCCCATCTTTATTCAACTTTCAGATGCATGGATATGCAGATCCAAGCATGTATGGTGCAGGTGCTGGCTTTCCATATGGGTTCCCTAACATGTTTCATGGTAGCCATGGTCACGCCCATGGATACTATCACCATCTCCGTCAAGGTCAGCAAGATTATTACCTGAAGCGGCTGCTTATGTTGATTGGTTTCTGTGTTCTTCTTGCCCTAATCTGGCAA

Protein sequence

MASGFGESTSRQPQSPSCSDNNSDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHIHSHPQECPVCKAIIEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPAGQRPEAAGPPPEPNHFPHNGFGFMGGLGGFAPMASTRFGNFTLSAAFGGLVPSLFNFQMHGYADPSMYGAGAGFPYGFPNMFHGSHGHAHGYYHHLRQGQQDYYLKRLLMLIGFCVLLALIWQ

Homology

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BLAST of MS016797 vs. NCBI nr
Match: XP_022144378.1 (E3 ubiquitin-protein ligase RNF185 [Momordica charantia])

HSP 1 Score: 486.9 bits (1252), Expect = 9.7e-134
Identity = 230/230 (100.00%), Postives = 230/230 (100.00%), Query Frame = 0


Query: 1   MASGFGESTSRQPQSPSCSDNNSDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHI 60
           MASGFGESTSRQPQSPSCSDNNSDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHI
Sbjct: 1   MASGFGESTSRQPQSPSCSDNNSDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHI 60

Query: 61  HSHPQECPVCKAIIEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPAGQRPEAAGPPPEP 120
           HSHPQECPVCKAIIEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPAGQRPEAAGPPPEP
Sbjct: 61  HSHPQECPVCKAIIEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPAGQRPEAAGPPPEP 120

Query: 121 NHFPHNGFGFMGGLGGFAPMASTRFGNFTLSAAFGGLVPSLFNFQMHGYADPSMYGAGAG 180
           NHFPHNGFGFMGGLGGFAPMASTRFGNFTLSAAFGGLVPSLFNFQMHGYADPSMYGAGAG
Sbjct: 121 NHFPHNGFGFMGGLGGFAPMASTRFGNFTLSAAFGGLVPSLFNFQMHGYADPSMYGAGAG 180

Query: 181 FPYGFPNMFHGSHGHAHGYYHHLRQGQQDYYLKRLLMLIGFCVLLALIWQ 231
           FPYGFPNMFHGSHGHAHGYYHHLRQGQQDYYLKRLLMLIGFCVLLALIWQ
Sbjct: 181 FPYGFPNMFHGSHGHAHGYYHHLRQGQQDYYLKRLLMLIGFCVLLALIWQ 230

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BLAST of MS016797 vs. NCBI nr
Match: XP_038884023.1 (E3 ubiquitin ligase rnf-5 [Benincasa hispida])

HSP 1 Score: 473.4 bits (1217), Expect = 1.1e-129
Identity = 224/230 (97.39%), Postives = 225/230 (97.83%), Query Frame = 0


Query: 1   MASGFGESTSRQPQSPSCSDNNSDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHI 60
           MASGFGESTSRQPQSPSCSDNN DASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHI
Sbjct: 1   MASGFGESTSRQPQSPSCSDNNGDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHI 60

Query: 61  HSHPQECPVCKAIIEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPAGQRPEAAGPPPEP 120
           HSHPQECPVCKAIIEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPAGQRPEAAGPPPEP
Sbjct: 61  HSHPQECPVCKAIIEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPAGQRPEAAGPPPEP 120

Query: 121 NHFPHNGFGFMGGLGGFAPMASTRFGNFTLSAAFGGLVPSLFNFQMHGYADPSMYGAGAG 180
           N FPHNGFGFMGGLGGFAPMASTRFGNFTLSAAFGGLVPSLFNFQMHGYADPSMYGAGA 
Sbjct: 121 NPFPHNGFGFMGGLGGFAPMASTRFGNFTLSAAFGGLVPSLFNFQMHGYADPSMYGAGAN 180

Query: 181 FPYGFPNMFHGSHGHAHGYYHHLRQGQQDYYLKRLLMLIGFCVLLALIWQ 231
           FPYGFPNMFHGSHGHAHGYYH LRQGQQDYYLKRLL+ IGFCVLLALIWQ
Sbjct: 181 FPYGFPNMFHGSHGHAHGYYHQLRQGQQDYYLKRLLLFIGFCVLLALIWQ 230

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BLAST of MS016797 vs. NCBI nr
Match: XP_004152714.1 (E3 ubiquitin ligase rnf-5 [Cucumis sativus] >XP_011649602.1 E3 ubiquitin ligase rnf-5 [Cucumis sativus] >KGN62568.1 hypothetical protein Csa_022553 [Cucumis sativus])

HSP 1 Score: 468.0 bits (1203), Expect = 4.7e-128
Identity = 223/230 (96.96%), Postives = 223/230 (96.96%), Query Frame = 0


Query: 1   MASGFGESTSRQPQSPSCSDNNSDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHI 60
           M SGFGESTSRQPQSPSCSDNN DASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHI
Sbjct: 1   MESGFGESTSRQPQSPSCSDNNGDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHI 60

Query: 61  HSHPQECPVCKAIIEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPAGQRPEAAGPPPEP 120
           HSHPQECPVCKAIIEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPAGQRPEAAGPPPEP
Sbjct: 61  HSHPQECPVCKAIIEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPAGQRPEAAGPPPEP 120

Query: 121 NHFPHNGFGFMGGLGGFAPMASTRFGNFTLSAAFGGLVPSLFNFQMHGYADPSMYGAGAG 180
           N FPHNGFGFMGGLGGFAPMASTRFGNFTLSAAFGGLVPSLFNFQMHGYADPSMYGAGA 
Sbjct: 121 NPFPHNGFGFMGGLGGFAPMASTRFGNFTLSAAFGGLVPSLFNFQMHGYADPSMYGAGAN 180

Query: 181 FPYGFPNMFHGSHGHAHGYYHHLRQGQQDYYLKRLLMLIGFCVLLALIWQ 231
           FPYGFPNMFHGSHGHAHGYYH LRQGQQDYYLKRLL LIGF VLLALIWQ
Sbjct: 181 FPYGFPNMFHGSHGHAHGYYHQLRQGQQDYYLKRLLFLIGFFVLLALIWQ 230

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BLAST of MS016797 vs. NCBI nr
Match: KAG6598412.1 (E3 ubiquitin ligase rnf-5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 467.2 bits (1201), Expect = 8.0e-128
Identity = 221/230 (96.09%), Postives = 223/230 (96.96%), Query Frame = 0


Query: 1   MASGFGESTSRQPQSPSCSDNNSDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHI 60
           MASGFGESTSRQPQ PS SDNN DASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHI
Sbjct: 17  MASGFGESTSRQPQGPSYSDNNGDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHI 76

Query: 61  HSHPQECPVCKAIIEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPAGQRPEAAGPPPEP 120
           HSHPQECPVCKA+IEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPAGQRPEAAGPPPEP
Sbjct: 77  HSHPQECPVCKALIEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPAGQRPEAAGPPPEP 136

Query: 121 NHFPHNGFGFMGGLGGFAPMASTRFGNFTLSAAFGGLVPSLFNFQMHGYADPSMYGAGAG 180
           N FPHNGFGFMGGLGGFAPMASTRFGNFTLSAAFGGLVPSLFNFQMHGYADPSMYGAGA 
Sbjct: 137 NPFPHNGFGFMGGLGGFAPMASTRFGNFTLSAAFGGLVPSLFNFQMHGYADPSMYGAGAN 196

Query: 181 FPYGFPNMFHGSHGHAHGYYHHLRQGQQDYYLKRLLMLIGFCVLLALIWQ 231
            PYGFPNMFHGSHGHAHGYYHHLRQGQQDYYLKRLL+ IGFCVLLALIWQ
Sbjct: 197 VPYGFPNMFHGSHGHAHGYYHHLRQGQQDYYLKRLLLFIGFCVLLALIWQ 246

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BLAST of MS016797 vs. NCBI nr
Match: XP_023547002.1 (E3 ubiquitin-protein ligase RNF185-like [Cucurbita pepo subsp. pepo] >XP_023547003.1 E3 ubiquitin-protein ligase RNF185-like [Cucurbita pepo subsp. pepo] >KAG7029364.1 RING finger protein 5, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 467.2 bits (1201), Expect = 8.0e-128
Identity = 221/230 (96.09%), Postives = 223/230 (96.96%), Query Frame = 0


Query: 1   MASGFGESTSRQPQSPSCSDNNSDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHI 60
           MASGFGESTSRQPQ PS SDNN DASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHI
Sbjct: 1   MASGFGESTSRQPQGPSYSDNNGDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHI 60

Query: 61  HSHPQECPVCKAIIEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPAGQRPEAAGPPPEP 120
           HSHPQECPVCKA+IEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPAGQRPEAAGPPPEP
Sbjct: 61  HSHPQECPVCKALIEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPAGQRPEAAGPPPEP 120

Query: 121 NHFPHNGFGFMGGLGGFAPMASTRFGNFTLSAAFGGLVPSLFNFQMHGYADPSMYGAGAG 180
           N FPHNGFGFMGGLGGFAPMASTRFGNFTLSAAFGGLVPSLFNFQMHGYADPSMYGAGA 
Sbjct: 121 NPFPHNGFGFMGGLGGFAPMASTRFGNFTLSAAFGGLVPSLFNFQMHGYADPSMYGAGAN 180

Query: 181 FPYGFPNMFHGSHGHAHGYYHHLRQGQQDYYLKRLLMLIGFCVLLALIWQ 231
            PYGFPNMFHGSHGHAHGYYHHLRQGQQDYYLKRLL+ IGFCVLLALIWQ
Sbjct: 181 VPYGFPNMFHGSHGHAHGYYHHLRQGQQDYYLKRLLLFIGFCVLLALIWQ 230

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BLAST of MS016797 vs. ExPASy Swiss-Prot
Match: Q09463 (E3 ubiquitin ligase rnf-5 OS=Caenorhabditis elegans OX=6239 GN=rnf-5 PE=1 SV=1)

HSP 1 Score: 137.1 bits (344), Expect = 2.5e-31
Identity = 90/242 (37.19%), Postives = 120/242 (49.59%), Query Frame = 0


Query: 9   TSRQPQSPSCSDNNSDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHIHSHPQECP 68
           T    + P+ S N  +++ FECNICLD A+D +V+LCGHLFCWPCL +WL    + Q CP
Sbjct: 5   TKAPSEEPTSSSNKDESARFECNICLDAAKDAVVSLCGHLFCWPCLSQWLDTRPNNQVCP 64

Query: 69  VCKAIIEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPAGQRPEAAGPPPEPNHFPHNGF 128
           VCK+ I+  K+VP+YGRG  S+DPR K      +P RP GQR E     P P  F    +
Sbjct: 65  VCKSAIDGNKVVPIYGRGGDSSDPRKK------VPPRPKGQRSE-----PPPQSFAGFNW 124

Query: 129 GFMGG-LGGFAPMASTRFGNFTLSAAF---------GGLVPSLFNFQMHGYADPSMYGAG 188
           G  GG +GG  P     FG  T++  F          G+ P  F     G  +     AG
Sbjct: 125 GGDGGMMGGGGPNVHFSFGIGTVNGLFPLMFMLPFIQGIFPLSFVASFFGNGNQGAAAAG 184

Query: 189 AGFPYGFPNMFHGSHGHAHGYYH-----------HLRQGQQDYYLKRLLMLIGFCVLLAL 230
            G   G      G+H H HG+ H             R  Q++ YL  +   IGF +L  L
Sbjct: 185 GGNGGGNDGN-DGTHAHTHGHTHGPRGHGESAAPGSRMAQEEEYLSNIFKYIGFFMLFWL 234

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BLAST of MS016797 vs. ExPASy Swiss-Prot
Match: Q5ZIR9 (E3 ubiquitin-protein ligase RNF185 OS=Gallus gallus OX=9031 GN=RNF185 PE=2 SV=1)

HSP 1 Score: 121.3 bits (303), Expect = 1.4e-26
Identity = 67/130 (51.54%), Postives = 79/130 (60.77%), Query Frame = 0


Query: 9   TSRQPQSPSCSDNNSDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHIHSHPQECP 68
           TS    S    DN +  + FECNICLD A+D +++LCGHLFCWPCL++WL    + Q CP
Sbjct: 20  TSGSSSSNGAGDNTNQDNTFECNICLDTAKDAVISLCGHLFCWPCLHQWLETRPNRQVCP 79

Query: 69  VCKAIIEEQKLVPLYGRGKT-STDPRSKSIPGINIPNRPAGQRPEAAGPPPEPNHFPHNG 128
           VCKA I   K++PLYGRG T   DPR K+      P RP GQRPE     PE N     G
Sbjct: 80  VCKAGISRDKVIPLYGRGSTGQQDPREKT------PPRPQGQRPE-----PE-NRGGFQG 135

Query: 129 FGFMGGLGGF 138
           FGF  G GGF
Sbjct: 140 FGF--GDGGF 135

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BLAST of MS016797 vs. ExPASy Swiss-Prot
Match: Q96GF1 (E3 ubiquitin-protein ligase RNF185 OS=Homo sapiens OX=9606 GN=RNF185 PE=1 SV=1)

HSP 1 Score: 117.1 bits (292), Expect = 2.7e-25
Identity = 71/143 (49.65%), Postives = 84/143 (58.74%), Query Frame = 0


Query: 6   GESTSRQPQS-----PSCSDNNS-----DASNFECNICLDLAQDPIVTLCGHLFCWPCLY 65
           G S S  P++     PS S N +       S FECNICLD A+D +++LCGHLFCWPCL+
Sbjct: 5   GPSASASPENSSAGGPSGSSNGAGESGGQDSTFECNICLDTAKDAVISLCGHLFCWPCLH 64

Query: 66  KWLHIHSHPQECPVCKAIIEEQKLVPLYGRGKT-STDPRSKSIPGINIPNRPAGQRPEAA 125
           +WL    + Q CPVCKA I   K++PLYGRG T   DPR K+      P RP GQRPE  
Sbjct: 65  QWLETRPNRQVCPVCKAGISRDKVIPLYGRGSTGQQDPREKT------PPRPQGQRPE-- 124

Query: 126 GPPPEPNHFPHNGFGFMGGLGGF 138
              PE N     GFGF  G GGF
Sbjct: 125 ---PE-NRGGFQGFGF--GDGGF 133

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BLAST of MS016797 vs. ExPASy Swiss-Prot
Match: Q5RFK9 (E3 ubiquitin-protein ligase RNF185 OS=Pongo abelii OX=9601 GN=RNF185 PE=2 SV=1)

HSP 1 Score: 117.1 bits (292), Expect = 2.7e-25
Identity = 71/143 (49.65%), Postives = 84/143 (58.74%), Query Frame = 0


Query: 6   GESTSRQPQS-----PSCSDNNS-----DASNFECNICLDLAQDPIVTLCGHLFCWPCLY 65
           G S S  P++     PS S N +       S FECNICLD A+D +++LCGHLFCWPCL+
Sbjct: 5   GPSASSSPENSSAGGPSGSSNGAGESGGQDSTFECNICLDTAKDAVISLCGHLFCWPCLH 64

Query: 66  KWLHIHSHPQECPVCKAIIEEQKLVPLYGRGKT-STDPRSKSIPGINIPNRPAGQRPEAA 125
           +WL    + Q CPVCKA I   K++PLYGRG T   DPR K+      P RP GQRPE  
Sbjct: 65  QWLETRPNRQVCPVCKAGISRDKVIPLYGRGSTGQQDPREKT------PPRPQGQRPE-- 124

Query: 126 GPPPEPNHFPHNGFGFMGGLGGF 138
              PE N     GFGF  G GGF
Sbjct: 125 ---PE-NRGGFQGFGF--GDGGF 133

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BLAST of MS016797 vs. ExPASy Swiss-Prot
Match: Q91YT2 (E3 ubiquitin-protein ligase RNF185 OS=Mus musculus OX=10090 GN=Rnf185 PE=2 SV=1)

HSP 1 Score: 116.7 bits (291), Expect = 3.5e-25
Identity = 64/124 (51.61%), Postives = 76/124 (61.29%), Query Frame = 0


Query: 15  SPSCSDNNSDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHIHSHPQECPVCKAII 74
           S    ++    S FECNICLD A+D +++LCGHLFCWPCL++WL    + Q CPVCKA I
Sbjct: 24  SNGTGESGGQDSTFECNICLDTAKDAVISLCGHLFCWPCLHQWLETRPNRQVCPVCKAGI 83

Query: 75  EEQKLVPLYGRGKT-STDPRSKSIPGINIPNRPAGQRPEAAGPPPEPNHFPHNGFGFMGG 134
              K++PLYGRG T   DPR K+      P RP GQRPE     PE N     GFGF  G
Sbjct: 84  SRDKVIPLYGRGSTGQQDPREKT------PPRPQGQRPE-----PE-NRGGFQGFGF--G 133

Query: 135 LGGF 138
            GGF
Sbjct: 144 DGGF 133

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BLAST of MS016797 vs. ExPASy TrEMBL
Match: A0A6J1CT42 (E3 ubiquitin-protein ligase RNF185 OS=Momordica charantia OX=3673 GN=LOC111014071 PE=4 SV=1)

HSP 1 Score: 486.9 bits (1252), Expect = 4.7e-134
Identity = 230/230 (100.00%), Postives = 230/230 (100.00%), Query Frame = 0


Query: 1   MASGFGESTSRQPQSPSCSDNNSDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHI 60
           MASGFGESTSRQPQSPSCSDNNSDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHI
Sbjct: 1   MASGFGESTSRQPQSPSCSDNNSDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHI 60

Query: 61  HSHPQECPVCKAIIEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPAGQRPEAAGPPPEP 120
           HSHPQECPVCKAIIEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPAGQRPEAAGPPPEP
Sbjct: 61  HSHPQECPVCKAIIEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPAGQRPEAAGPPPEP 120

Query: 121 NHFPHNGFGFMGGLGGFAPMASTRFGNFTLSAAFGGLVPSLFNFQMHGYADPSMYGAGAG 180
           NHFPHNGFGFMGGLGGFAPMASTRFGNFTLSAAFGGLVPSLFNFQMHGYADPSMYGAGAG
Sbjct: 121 NHFPHNGFGFMGGLGGFAPMASTRFGNFTLSAAFGGLVPSLFNFQMHGYADPSMYGAGAG 180

Query: 181 FPYGFPNMFHGSHGHAHGYYHHLRQGQQDYYLKRLLMLIGFCVLLALIWQ 231
           FPYGFPNMFHGSHGHAHGYYHHLRQGQQDYYLKRLLMLIGFCVLLALIWQ
Sbjct: 181 FPYGFPNMFHGSHGHAHGYYHHLRQGQQDYYLKRLLMLIGFCVLLALIWQ 230

[Close]

BLAST of MS016797 vs. ExPASy TrEMBL
Match: A0A0A0LLD2 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G360850 PE=4 SV=1)

HSP 1 Score: 468.0 bits (1203), Expect = 2.3e-128
Identity = 223/230 (96.96%), Postives = 223/230 (96.96%), Query Frame = 0


Query: 1   MASGFGESTSRQPQSPSCSDNNSDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHI 60
           M SGFGESTSRQPQSPSCSDNN DASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHI
Sbjct: 1   MESGFGESTSRQPQSPSCSDNNGDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHI 60

Query: 61  HSHPQECPVCKAIIEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPAGQRPEAAGPPPEP 120
           HSHPQECPVCKAIIEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPAGQRPEAAGPPPEP
Sbjct: 61  HSHPQECPVCKAIIEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPAGQRPEAAGPPPEP 120

Query: 121 NHFPHNGFGFMGGLGGFAPMASTRFGNFTLSAAFGGLVPSLFNFQMHGYADPSMYGAGAG 180
           N FPHNGFGFMGGLGGFAPMASTRFGNFTLSAAFGGLVPSLFNFQMHGYADPSMYGAGA 
Sbjct: 121 NPFPHNGFGFMGGLGGFAPMASTRFGNFTLSAAFGGLVPSLFNFQMHGYADPSMYGAGAN 180

Query: 181 FPYGFPNMFHGSHGHAHGYYHHLRQGQQDYYLKRLLMLIGFCVLLALIWQ 231
           FPYGFPNMFHGSHGHAHGYYH LRQGQQDYYLKRLL LIGF VLLALIWQ
Sbjct: 181 FPYGFPNMFHGSHGHAHGYYHQLRQGQQDYYLKRLLFLIGFFVLLALIWQ 230

[Close]

BLAST of MS016797 vs. ExPASy TrEMBL
Match: A0A1S3BBQ8 (RING finger protein 5 OS=Cucumis melo OX=3656 GN=LOC103487942 PE=4 SV=1)

HSP 1 Score: 466.8 bits (1200), Expect = 5.0e-128
Identity = 222/230 (96.52%), Postives = 223/230 (96.96%), Query Frame = 0


Query: 1   MASGFGESTSRQPQSPSCSDNNSDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHI 60
           MASG GESTSRQPQSPSCSDNN DASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHI
Sbjct: 1   MASGLGESTSRQPQSPSCSDNNGDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHI 60

Query: 61  HSHPQECPVCKAIIEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPAGQRPEAAGPPPEP 120
           HSHPQECPVCKAIIEEQKLVPLYGRGKTSTDPRSK IPGINIPNRPAGQRPEAAGPPPEP
Sbjct: 61  HSHPQECPVCKAIIEEQKLVPLYGRGKTSTDPRSKGIPGINIPNRPAGQRPEAAGPPPEP 120

Query: 121 NHFPHNGFGFMGGLGGFAPMASTRFGNFTLSAAFGGLVPSLFNFQMHGYADPSMYGAGAG 180
           N FPHNGFGFMGGLGGFAPMASTRFGNFTLSAAFGGLVPSLFNFQMHGYADPSMYGAGA 
Sbjct: 121 NPFPHNGFGFMGGLGGFAPMASTRFGNFTLSAAFGGLVPSLFNFQMHGYADPSMYGAGAN 180

Query: 181 FPYGFPNMFHGSHGHAHGYYHHLRQGQQDYYLKRLLMLIGFCVLLALIWQ 231
           FPYGFPNMFHGSHGHAHGYYH LRQGQQDYYLKRLL+LIGF VLLALIWQ
Sbjct: 181 FPYGFPNMFHGSHGHAHGYYHQLRQGQQDYYLKRLLLLIGFFVLLALIWQ 230

[Close]

BLAST of MS016797 vs. ExPASy TrEMBL
Match: A0A5A7VHN4 (RING finger protein 5 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G006030 PE=4 SV=1)

HSP 1 Score: 466.8 bits (1200), Expect = 5.0e-128
Identity = 222/230 (96.52%), Postives = 223/230 (96.96%), Query Frame = 0


Query: 1   MASGFGESTSRQPQSPSCSDNNSDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHI 60
           MASG GESTSRQPQSPSCSDNN DASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHI
Sbjct: 1   MASGLGESTSRQPQSPSCSDNNGDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHI 60

Query: 61  HSHPQECPVCKAIIEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPAGQRPEAAGPPPEP 120
           HSHPQECPVCKAIIEEQKLVPLYGRGKTSTDPRSK IPGINIPNRPAGQRPEAAGPPPEP
Sbjct: 61  HSHPQECPVCKAIIEEQKLVPLYGRGKTSTDPRSKGIPGINIPNRPAGQRPEAAGPPPEP 120

Query: 121 NHFPHNGFGFMGGLGGFAPMASTRFGNFTLSAAFGGLVPSLFNFQMHGYADPSMYGAGAG 180
           N FPHNGFGFMGGLGGFAPMASTRFGNFTLSAAFGGLVPSLFNFQMHGYADPSMYGAGA 
Sbjct: 121 NPFPHNGFGFMGGLGGFAPMASTRFGNFTLSAAFGGLVPSLFNFQMHGYADPSMYGAGAN 180

Query: 181 FPYGFPNMFHGSHGHAHGYYHHLRQGQQDYYLKRLLMLIGFCVLLALIWQ 231
           FPYGFPNMFHGSHGHAHGYYH LRQGQQDYYLKRLL+LIGF VLLALIWQ
Sbjct: 181 FPYGFPNMFHGSHGHAHGYYHQLRQGQQDYYLKRLLLLIGFFVLLALIWQ 230

[Close]

BLAST of MS016797 vs. ExPASy TrEMBL
Match: A0A6J1HCN0 (E3 ubiquitin-protein ligase RNF185-like OS=Cucurbita moschata OX=3662 GN=LOC111462860 PE=4 SV=1)

HSP 1 Score: 466.1 bits (1198), Expect = 8.6e-128
Identity = 220/230 (95.65%), Postives = 223/230 (96.96%), Query Frame = 0


Query: 1   MASGFGESTSRQPQSPSCSDNNSDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHI 60
           MASGFGESTSRQPQ PS SDNN DASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHI
Sbjct: 1   MASGFGESTSRQPQGPSYSDNNGDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHI 60

Query: 61  HSHPQECPVCKAIIEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPAGQRPEAAGPPPEP 120
           HSHPQECPVCKA+IEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRP+GQRPEAAGPPPEP
Sbjct: 61  HSHPQECPVCKALIEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPSGQRPEAAGPPPEP 120

Query: 121 NHFPHNGFGFMGGLGGFAPMASTRFGNFTLSAAFGGLVPSLFNFQMHGYADPSMYGAGAG 180
           N FPHNGFGFMGGLGGFAPMASTRFGNFTLSAAFGGLVPSLFNFQMHGYADPSMYGAGA 
Sbjct: 121 NPFPHNGFGFMGGLGGFAPMASTRFGNFTLSAAFGGLVPSLFNFQMHGYADPSMYGAGAN 180

Query: 181 FPYGFPNMFHGSHGHAHGYYHHLRQGQQDYYLKRLLMLIGFCVLLALIWQ 231
            PYGFPNMFHGSHGHAHGYYHHLRQGQQDYYLKRLL+ IGFCVLLALIWQ
Sbjct: 181 VPYGFPNMFHGSHGHAHGYYHHLRQGQQDYYLKRLLLFIGFCVLLALIWQ 230

[Close]

BLAST of MS016797 vs. TAIR 10
Match: AT1G19310.1 (RING/U-box superfamily protein )

HSP 1 Score: 298.1 bits (762), Expect = 6.0e-81
Identity = 148/220 (67.27%), Postives = 174/220 (79.09%), Query Frame = 0


Query: 16  PSCSD----NNSDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHIHSHPQECPVCK 75
           PSCS     NN+D+SNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLH+HS  ++CPVCK
Sbjct: 5   PSCSSGNDTNNNDSSNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHLHSQSKDCPVCK 64

Query: 76  AIIEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPAGQRPEAAGPPPEPNHFPHNGFGFM 135
           A+IEE +LVPLYGRGK+S DPRSKSIPG+ +PNRP+GQRPE A  PP+PNH   +  GF 
Sbjct: 65  AVIEEDRLVPLYGRGKSSADPRSKSIPGLEVPNRPSGQRPETA-QPPDPNHGFAHHHGFG 124

Query: 136 GGLGGF-APMASTRFGNFTLSAAFGGLVPSLFNFQMHGYADPSMYGAGA--GFPYGFPNM 195
           G +GGF APMAS RFGN TLSAAFGGL+PSLFN   HG+ D +MYGA A  GFP+GF N 
Sbjct: 125 GFMGGFAAPMASARFGNVTLSAAFGGLIPSLFNLHFHGFPDAAMYGAAASGGFPHGFSNP 184

Query: 196 FHGSHGHAHGYYHHL-RQGQQDYYLKRLLMLIGFCVLLAL 228
           FHG H H H Y  H  RQGQQD++L+ LL+++   V+ +L
Sbjct: 185 FHGGHSHMHSYQRHTGRQGQQDHHLRILLLIVFVVVVFSL 223

[Close]

BLAST of MS016797 vs. TAIR 10
Match: AT2G23780.1 (RING/U-box superfamily protein )

HSP 1 Score: 256.5 bits (654), Expect = 2.0e-68
Identity = 131/230 (56.96%), Postives = 162/230 (70.43%), Query Frame = 0


Query: 6   GESTSRQPQSPSCSDNNSDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHIHSHPQ 65
           GES++    S S +D N    +FECNIC +LAQDPIVTLCGHLFCWPCLY+WLH HSH Q
Sbjct: 4   GESSTSTSYSDSNNDTNDQGGDFECNICFELAQDPIVTLCGHLFCWPCLYRWLHHHSHSQ 63

Query: 66  ECPVCKAIIEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPAGQRPEAAGPPPEP---NH 125
           ECPVCKA++++ KLVPLYGRGK  TDPRSK  PG+ IPNRP GQRPE A PPP+P   ++
Sbjct: 64  ECPVCKAVVQDDKLVPLYGRGKNQTDPRSKRYPGLRIPNRPTGQRPETAAPPPQPEAASN 123

Query: 126 FPHNGFGFMGGLGGFAPMASTRFGNFTLSAAFGGLVPSLFNFQMHGYADPSMYGAGAGFP 185
           F + G G MGG+     MA+TRFGNF  S  FGGL+PSLFNFQ HG+ D ++YG+  G+P
Sbjct: 124 FFNYGIGLMGGI--MPMMATTRFGNF--SMGFGGLLPSLFNFQFHGFHDATLYGSTPGYP 183

Query: 186 Y-GFPNMFHGSHGHAHGYYHHLRQG--QQDYYLKRLLMLIGFCVLLALIW 230
           Y G+ N F G      G    + +G  Q D +LK +L  +G CV++ LIW
Sbjct: 184 YGGYHNGFRGV--PPRGQERPMARGGNQSDAFLKNILFFVGICVVIFLIW 227

[Close]

BLAST of MS016797 vs. TAIR 10
Match: AT1G74990.1 (RING/U-box superfamily protein )

HSP 1 Score: 157.5 bits (397), Expect = 1.3e-38
Identity = 76/133 (57.14%), Postives = 91/133 (68.42%), Query Frame = 0


Query: 19  SDNNSDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHIHSHPQECPVCKAIIEEQK 78
           ++ +  ++NF CNICL+LA++PIVTLCGHLFCWPCLYKWLH HS    CPVCKA+++E  
Sbjct: 8   NEEDDASNNFGCNICLELAREPIVTLCGHLFCWPCLYKWLHYHSKSNHCPVCKALVKEDT 67

Query: 79  LVPLYGRGKTSTDPRSKSIPGINIPNRPAGQRPEAAGPPPEPNHFPHNGFGFMGGLGGFA 138
           LVPLYG GK S+DPRSK   G+ +PNRPA  R E A P  E     H+G  F GG   FA
Sbjct: 68  LVPLYGMGKPSSDPRSKLNSGVTVPNRPAATRTETARPRLEQR---HHGSSFFGGHSSFA 127

Query: 139 PM-ASTRFGNFTL 151
            M    RF NF L
Sbjct: 128 AMPTGLRFSNFLL 137

[Close]

BLAST of MS016797 vs. TAIR 10
Match: AT4G03510.1 (RING membrane-anchor 1 )

HSP 1 Score: 110.5 bits (275), Expect = 1.8e-24
Identity = 50/110 (45.45%), Postives = 71/110 (64.55%), Query Frame = 0


Query: 7   ESTSRQPQSPSCSDNNSDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHIHS---- 66
           +S   +P + S   +++D SNF+CNICLD  Q+P+VTLCGHLFCWPC++KWL + S    
Sbjct: 25  KSKKPEPITVSVPSDDTDDSNFDCNICLDSVQEPVVTLCGHLFCWPCIHKWLDVQSFSTS 84

Query: 67  ----HPQECPVCKAIIEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPAG 109
                 ++CPVCK+ +    LVPLYGRG+ +T    K+    ++P RP G
Sbjct: 85  DEYQRHRQCPVCKSKVSHSTLVPLYGRGRCTTQEEGKN----SVPKRPVG 130

[Close]

BLAST of MS016797 vs. TAIR 10
Match: AT4G03510.2 (RING membrane-anchor 1 )

HSP 1 Score: 110.5 bits (275), Expect = 1.8e-24
Identity = 50/110 (45.45%), Postives = 71/110 (64.55%), Query Frame = 0


Query: 7   ESTSRQPQSPSCSDNNSDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHIHS---- 66
           +S   +P + S   +++D SNF+CNICLD  Q+P+VTLCGHLFCWPC++KWL + S    
Sbjct: 25  KSKKPEPITVSVPSDDTDDSNFDCNICLDSVQEPVVTLCGHLFCWPCIHKWLDVQSFSTS 84

Query: 67  ----HPQECPVCKAIIEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPAG 109
                 ++CPVCK+ +    LVPLYGRG+ +T    K+    ++P RP G
Sbjct: 85  DEYQRHRQCPVCKSKVSHSTLVPLYGRGRCTTQEEGKN----SVPKRPVG 130

The following BLAST results are available for this feature:

BLAST of MS016797 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022144378.1	9.7e-134	100.00	E3 ubiquitin-protein ligase RNF185 [Momordica charantia]	[more]
XP_038884023.1	1.1e-129	97.39	E3 ubiquitin ligase rnf-5 [Benincasa hispida]	[more]
XP_004152714.1	4.7e-128	96.96	E3 ubiquitin ligase rnf-5 [Cucumis sativus] >XP_011649602.1 E3 ubiquitin ligase ...	[more]
KAG6598412.1	8.0e-128	96.09	E3 ubiquitin ligase rnf-5, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_023547002.1	8.0e-128	96.09	E3 ubiquitin-protein ligase RNF185-like [Cucurbita pepo subsp. pepo] >XP_0235470...	[more]

BLAST of MS016797 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q09463	2.5e-31	37.19	E3 ubiquitin ligase rnf-5 OS=Caenorhabditis elegans OX=6239 GN=rnf-5 PE=1 SV=1	[more]
Q5ZIR9	1.4e-26	51.54	E3 ubiquitin-protein ligase RNF185 OS=Gallus gallus OX=9031 GN=RNF185 PE=2 SV=1	[more]
Q96GF1	2.7e-25	49.65	E3 ubiquitin-protein ligase RNF185 OS=Homo sapiens OX=9606 GN=RNF185 PE=1 SV=1	[more]
Q5RFK9	2.7e-25	49.65	E3 ubiquitin-protein ligase RNF185 OS=Pongo abelii OX=9601 GN=RNF185 PE=2 SV=1	[more]
Q91YT2	3.5e-25	51.61	E3 ubiquitin-protein ligase RNF185 OS=Mus musculus OX=10090 GN=Rnf185 PE=2 SV=1	[more]

BLAST of MS016797 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CT42	4.7e-134	100.00	E3 ubiquitin-protein ligase RNF185 OS=Momordica charantia OX=3673 GN=LOC11101407...	[more]
A0A0A0LLD2	2.3e-128	96.96	RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G360850 P...	[more]
A0A1S3BBQ8	5.0e-128	96.52	RING finger protein 5 OS=Cucumis melo OX=3656 GN=LOC103487942 PE=4 SV=1	[more]
A0A5A7VHN4	5.0e-128	96.52	RING finger protein 5 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82...	[more]
A0A6J1HCN0	8.6e-128	95.65	E3 ubiquitin-protein ligase RNF185-like OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]

BLAST of MS016797 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G19310.1	6.0e-81	67.27	RING/U-box superfamily protein	[more]
AT2G23780.1	2.0e-68	56.96	RING/U-box superfamily protein	[more]
AT1G74990.1	1.3e-38	57.14	RING/U-box superfamily protein	[more]
AT4G03510.1	1.8e-24	45.45	RING membrane-anchor 1	[more]
AT4G03510.2	1.8e-24	45.45	RING membrane-anchor 1	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 30..70 e-value: 6.4E-8 score: 42.3
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 30..71 score: 13.256433
None	No IPR available	PFAM	PF13923	zf-C3HC4_2	coord: 29..70 e-value: 1.9E-8 score: 34.0
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 84..121
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..20
None	No IPR available	PANTHER	PTHR12313:SF39	OS12G0636000 PROTEIN	coord: 2..230
None	No IPR available	CDD	cd16745	RING-HC_AtRMA_like	coord: 28..72 e-value: 2.06306E-30 score: 105.079
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 26..83
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 5..96 e-value: 4.0E-17 score: 64.3
IPR045103	E3 ubiquitin-protein ligase RNF5/RNF185-like	PANTHER	PTHR12313	E3 UBIQUITIN-PROTEIN LIGASE RNF5-RELATED	coord: 2..230
IPR017907	Zinc finger, RING-type, conserved site	PROSITE	PS00518	ZF_RING_1	coord: 45..54

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS016797.1	MS016797.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006511	ubiquitin-dependent protein catabolic process
cellular_component	GO:0005783	endoplasmic reticulum
molecular_function	GO:0061630	ubiquitin protein ligase activity