MS016760 (gene) Bitter gourd (TR) v1

BLAST of MS016760 vs. NCBI nr
Match: XP_022157325.1 (protein SIEL isoform X1 [Momordica charantia])

HSP 1 Score: 1602.8 bits (4149), Expect = 0.0e+00
Identity = 805/815 (98.77%), Postives = 808/815 (99.14%), Query Frame = 0

Query: 1   MAERDRELVSAIDELDDGSFLSLCFGPSVSIRTWLLNNAERFQIRPSLLFTVFLGFTKDP 60
           MAERDRELVSAIDELDDGSFLSLCFGPSVSIRTWLLNNAERFQIRPSLLFTVFLGFTKDP
Sbjct: 1   MAERDRELVSAIDELDDGSFLSLCFGPSVSIRTWLLNNAERFQIRPSLLFTVFLGFTKDP 60

Query: 61  YPYVRKAALDGLVGLGNNVFEDGGMIEGCYYRAIELLNDMEDCVRSAAVRVVITWGLMLA 120
           YPYVR AALDGLVGLGNNVFEDGGMIEGCYYRAIELLNDMEDCVRSAAVRVVITWGLMLA
Sbjct: 61  YPYVRIAALDGLVGLGNNVFEDGGMIEGCYYRAIELLNDMEDCVRSAAVRVVITWGLMLA 120

Query: 121 AHSPERKRYLSDEIFINLCSMTRDMSMKVRVNAFDAIKKLEIVSEDILLQSVSKRVLGIF 180
           AHSPERKRYLSDEIFINLCSMTRDMSMKVRVNAFDAIKKLEIVSEDILLQSVSK+VLGIF
Sbjct: 121 AHSPERKRYLSDEIFINLCSMTRDMSMKVRVNAFDAIKKLEIVSEDILLQSVSKKVLGIF 180

Query: 181 KGKRPLVQCSTEQLEMLALDVAGAFVHGVEDEFYQVRKSACDALYNLPILSTKFAGESLN 240
           KGK+PLVQCSTEQLEMLALDVAGAFVHGVEDEFYQVRKSACDALYNLPILSTKFAGE+LN
Sbjct: 181 KGKKPLVQCSTEQLEMLALDVAGAFVHGVEDEFYQVRKSACDALYNLPILSTKFAGEALN 240

Query: 241 LLMDVLNDDSVSVRLQALETLHHMAMSKCLKLQEAHMHMFLGALSDSNGHVRSALRKLLK 300
           LLMDVLNDDSVSVRLQALETLHHMAMSKCLKLQEAHMHMFLGALSDSNGHVRSALRKLLK
Sbjct: 241 LLMDVLNDDSVSVRLQALETLHHMAMSKCLKLQEAHMHMFLGALSDSNGHVRSALRKLLK 300

Query: 301 LAKLPDLETFQLSFNGLLKSLESYPQDESDVLSVLFHMGQNHVNMAASIIQGVFKQIDPT 360
           L KLPDLETFQLSFNGLLKSLESY QDES VLSVLFHMGQNHVNMAASIIQGVFKQIDPT
Sbjct: 301 LVKLPDLETFQLSFNGLLKSLESYAQDESGVLSVLFHMGQNHVNMAASIIQGVFKQIDPT 360

Query: 361 SEGELGFDSVKVTAYIVLAISAPVLDDHTLRIPSRLFSYAVTLLGKISHALGDIMDQSTF 420
           SEGELGFDSVKVTAYIVLAISAPVLDDHTLRIPSRLFSYAVTLLGKISHALGDIMDQSTF
Sbjct: 361 SEGELGFDSVKVTAYIVLAISAPVLDDHTLRIPSRLFSYAVTLLGKISHALGDIMDQSTF 420

Query: 421 FAYLLENCKNIGSSDLVLNPEGSPCSPTPGGSINDILVSLRTTAMINEQQHKHDDTIESI 480
           FAYLLENCKNIGSSDLVLNPEGSPCSPTPGGSINDILVSLRTTAMINEQQHKHDDTIESI
Sbjct: 421 FAYLLENCKNIGSSDLVLNPEGSPCSPTPGGSINDILVSLRTTAMINEQQHKHDDTIESI 480

Query: 481 KTVLSKVQDIWPLIQSGFLHQVLRTLRFCKEALGTFTHGSDTSSGALAFTLQYLKIIKLV 540
           KTVLSKVQDIWPLIQSGFLHQVLRTLRFCKEALGTFTHGSDTSSGALAFTLQYLKIIKLV
Sbjct: 481 KTVLSKVQDIWPLIQSGFLHQVLRTLRFCKEALGTFTHGSDTSSGALAFTLQYLKIIKLV 540

Query: 541 AKVWNLMSAKLNCLRKIGEWELLLGKLERGLKELRSRFIGFSKEEELHILELMLVTYTLR 600
           AKVWNLMSAKLNCLRKIGEWELLLGKLERGLKELRSRFIGFSKEEELHILELMLVTYTLR
Sbjct: 541 AKVWNLMSAKLNCLRKIGEWELLLGKLERGLKELRSRFIGFSKEEELHILELMLVTYTLR 600

Query: 601 LYNGEVWCHLMTLRKLSIIASDIEHLLKEGSFEPSTFVFEAQKALSSLGTVTPKASYNLF 660
           LYNGEVWCHLMTLRKLSIIASDIEHLLKEGSFEPSTFVFEAQKALSSLGTVTPKASYNLF
Sbjct: 601 LYNGEVWCHLMTLRKLSIIASDIEHLLKEGSFEPSTFVFEAQKALSSLGTVTPKASYNLF 660

Query: 661 DFRKLLQSFTLNHLEFSGKLKHIKAELVILDNDYEKPLHFVPGLPVGIPCQIILHNVLSE 720
           DFRKLLQSFTLNHLEFSGKLKHIKAEL ILDNDYEKPLHFVPGLPVGIPCQIILHNVLSE
Sbjct: 661 DFRKLLQSFTLNHLEFSGKLKHIKAELFILDNDYEKPLHFVPGLPVGIPCQIILHNVLSE 720

Query: 721 RKLWVRISMDGMTSQFVFLDFPVFGGCDEVREFTYVVPFYRTPKASSFIARICIGLECCF 780
           RKLWVRISMDGMTSQFVFLDFPVFGGCDEVREFTYVVPFYRTPKASSFIARICIGLECCF
Sbjct: 721 RKLWVRISMDGMTSQFVFLDFPVFGGCDEVREFTYVVPFYRTPKASSFIARICIGLECCF 780

Query: 781 ESAEVCKRHRHGGPRRDLAYICKEKEVYLSMINKG 816
           ESAEV KRH HGGPRRDLAYICKEKEVYLSMINKG
Sbjct: 781 ESAEVSKRHGHGGPRRDLAYICKEKEVYLSMINKG 815

BLAST of MS016760 vs. NCBI nr
Match: XP_022157326.1 (protein SIEL isoform X2 [Momordica charantia])

HSP 1 Score: 1366.3 bits (3535), Expect = 0.0e+00
Identity = 689/698 (98.71%), Postives = 692/698 (99.14%), Query Frame = 0

Query: 118 MLAAHSPERKRYLSDEIFINLCSMTRDMSMKVRVNAFDAIKKLEIVSEDILLQSVSKRVL 177
           MLAAHSPERKRYLSDEIFINLCSMTRDMSMKVRVNAFDAIKKLEIVSEDILLQSVSK+VL
Sbjct: 1   MLAAHSPERKRYLSDEIFINLCSMTRDMSMKVRVNAFDAIKKLEIVSEDILLQSVSKKVL 60

Query: 178 GIFKGKRPLVQCSTEQLEMLALDVAGAFVHGVEDEFYQVRKSACDALYNLPILSTKFAGE 237
           GIFKGK+PLVQCSTEQLEMLALDVAGAFVHGVEDEFYQVRKSACDALYNLPILSTKFAGE
Sbjct: 61  GIFKGKKPLVQCSTEQLEMLALDVAGAFVHGVEDEFYQVRKSACDALYNLPILSTKFAGE 120

Query: 238 SLNLLMDVLNDDSVSVRLQALETLHHMAMSKCLKLQEAHMHMFLGALSDSNGHVRSALRK 297
           +LNLLMDVLNDDSVSVRLQALETLHHMAMSKCLKLQEAHMHMFLGALSDSNGHVRSALRK
Sbjct: 121 ALNLLMDVLNDDSVSVRLQALETLHHMAMSKCLKLQEAHMHMFLGALSDSNGHVRSALRK 180

Query: 298 LLKLAKLPDLETFQLSFNGLLKSLESYPQDESDVLSVLFHMGQNHVNMAASIIQGVFKQI 357
           LLKL KLPDLETFQLSFNGLLKSLESY QDES VLSVLFHMGQNHVNMAASIIQGVFKQI
Sbjct: 181 LLKLVKLPDLETFQLSFNGLLKSLESYAQDESGVLSVLFHMGQNHVNMAASIIQGVFKQI 240

Query: 358 DPTSEGELGFDSVKVTAYIVLAISAPVLDDHTLRIPSRLFSYAVTLLGKISHALGDIMDQ 417
           DPTSEGELGFDSVKVTAYIVLAISAPVLDDHTLRIPSRLFSYAVTLLGKISHALGDIMDQ
Sbjct: 241 DPTSEGELGFDSVKVTAYIVLAISAPVLDDHTLRIPSRLFSYAVTLLGKISHALGDIMDQ 300

Query: 418 STFFAYLLENCKNIGSSDLVLNPEGSPCSPTPGGSINDILVSLRTTAMINEQQHKHDDTI 477
           STFFAYLLENCKNIGSSDLVLNPEGSPCSPTPGGSINDILVSLRTTAMINEQQHKHDDTI
Sbjct: 301 STFFAYLLENCKNIGSSDLVLNPEGSPCSPTPGGSINDILVSLRTTAMINEQQHKHDDTI 360

Query: 478 ESIKTVLSKVQDIWPLIQSGFLHQVLRTLRFCKEALGTFTHGSDTSSGALAFTLQYLKII 537
           ESIKTVLSKVQDIWPLIQSGFLHQVLRTLRFCKEALGTFTHGSDTSSGALAFTLQYLKII
Sbjct: 361 ESIKTVLSKVQDIWPLIQSGFLHQVLRTLRFCKEALGTFTHGSDTSSGALAFTLQYLKII 420

Query: 538 KLVAKVWNLMSAKLNCLRKIGEWELLLGKLERGLKELRSRFIGFSKEEELHILELMLVTY 597
           KLVAKVWNLMSAKLNCLRKIGEWELLLGKLERGLKELRSRFIGFSKEEELHILELMLVTY
Sbjct: 421 KLVAKVWNLMSAKLNCLRKIGEWELLLGKLERGLKELRSRFIGFSKEEELHILELMLVTY 480

Query: 598 TLRLYNGEVWCHLMTLRKLSIIASDIEHLLKEGSFEPSTFVFEAQKALSSLGTVTPKASY 657
           TLRLYNGEVWCHLMTLRKLSIIASDIEHLLKEGSFEPSTFVFEAQKALSSLGTVTPKASY
Sbjct: 481 TLRLYNGEVWCHLMTLRKLSIIASDIEHLLKEGSFEPSTFVFEAQKALSSLGTVTPKASY 540

Query: 658 NLFDFRKLLQSFTLNHLEFSGKLKHIKAELVILDNDYEKPLHFVPGLPVGIPCQIILHNV 717
           NLFDFRKLLQSFTLNHLEFSGKLKHIKAEL ILDNDYEKPLHFVPGLPVGIPCQIILHNV
Sbjct: 541 NLFDFRKLLQSFTLNHLEFSGKLKHIKAELFILDNDYEKPLHFVPGLPVGIPCQIILHNV 600

Query: 718 LSERKLWVRISMDGMTSQFVFLDFPVFGGCDEVREFTYVVPFYRTPKASSFIARICIGLE 777
           LSERKLWVRISMDGMTSQFVFLDFPVFGGCDEVREFTYVVPFYRTPKASSFIARICIGLE
Sbjct: 601 LSERKLWVRISMDGMTSQFVFLDFPVFGGCDEVREFTYVVPFYRTPKASSFIARICIGLE 660

Query: 778 CCFESAEVCKRHRHGGPRRDLAYICKEKEVYLSMINKG 816
           CCFESAEV KRH HGGPRRDLAYICKEKEVYLSMINKG
Sbjct: 661 CCFESAEVSKRHGHGGPRRDLAYICKEKEVYLSMINKG 698

BLAST of MS016760 vs. NCBI nr
Match: XP_038894154.1 (protein SIEL [Benincasa hispida])

HSP 1 Score: 1309.7 bits (3388), Expect = 0.0e+00
Identity = 659/817 (80.66%), Postives = 728/817 (89.11%), Query Frame = 0

Query: 1   MAERDRELVSAIDELDDGSFLSLCFGPSVSIRTWLLNNAERFQIRPSLLFTVFLGFTKDP 60
           M ERD ELVSAI+ELDD SFLSLCFGPSVSIRTWLLNNAERFQ+RPSLLFTVFLGFTKDP
Sbjct: 1   MEERDLELVSAINELDDQSFLSLCFGPSVSIRTWLLNNAERFQLRPSLLFTVFLGFTKDP 60

Query: 61  YPYVRKAALDGLVGLGNNVFEDGGMIEGCYYRAIELLNDMEDCVRSAAVRVVITWGLMLA 120
           YPYVRKAALDGL+GLGN V EDG MIEGCY R+IELLNDMEDCVRSAAV VVITWGLMLA
Sbjct: 61  YPYVRKAALDGLLGLGNTVLEDGSMIEGCYCRSIELLNDMEDCVRSAAVSVVITWGLMLA 120

Query: 121 AHSPERKRYLSDEIFINLCSMTRDMSMKVRVNAFDAIKKLEIVSEDILLQSVSKRVLGIF 180
           AHSP RK++LSDEIF+NLCSMTRDM+MKVRVNAFDA+K+LEIVSED+LLQSVSKRVL IF
Sbjct: 121 AHSPGRKQHLSDEIFVNLCSMTRDMNMKVRVNAFDAMKRLEIVSEDLLLQSVSKRVLSIF 180

Query: 181 KGKRPLVQCSTEQLEMLALDVAGAFVHGVEDEFYQVRKSACDALYNLPILSTKFAGESLN 240
           KGK+ LVQCSTEQLEM ALDVAGAFVHGVEDEFYQVR+SACDAL+NL ILSTKFAGE+L+
Sbjct: 181 KGKKSLVQCSTEQLEMSALDVAGAFVHGVEDEFYQVRRSACDALFNLTILSTKFAGEALS 240

Query: 241 LLMDVLNDDSVSVRLQALETLHHMAMSKCLKLQEAHMHMFLGALSDSNGHVRSALRKLLK 300
           LLMD+LNDDSVSVRL+ALETLHHMAM  CLKLQEAHMHMFL AL+D++GHVRSALRKLLK
Sbjct: 241 LLMDLLNDDSVSVRLRALETLHHMAMCNCLKLQEAHMHMFLSALNDNDGHVRSALRKLLK 300

Query: 301 LAKLPDLETFQLSFNGLLKSLESYPQDESDVLSVLFHMGQNHVNMAASIIQGVFKQIDPT 360
           +AKLPDL TFQLSFNGLL+SLESYPQDESDVLSVLFHMGQNHVNM  SII+ VF+QIDPT
Sbjct: 301 VAKLPDLVTFQLSFNGLLESLESYPQDESDVLSVLFHMGQNHVNMVDSIIKDVFEQIDPT 360

Query: 361 SEGELGFDSVKVTAYIVLAISAPVLDDHTLRIPSRLFSYAVTLLGKISHALGDIMDQSTF 420
           SEG+LGFDS KV AYIVLAISAPV D+HT RIP R+FSYA T+LG+ISHALGDIMDQ+T 
Sbjct: 361 SEGKLGFDSAKVIAYIVLAISAPVSDNHTRRIPPRIFSYAATMLGRISHALGDIMDQTTV 420

Query: 421 FAYLLENCKNIGSSDLVLNPEGSPCSPTPGGSINDI--LVSLRTTAMINEQQHKHDDTIE 480
           FAYLL+N K+IG SDL  NPEG PCSPTPG S+ND+  + SL+  AMI+EQ+ K DD IE
Sbjct: 421 FAYLLQNSKHIGLSDLGFNPEGDPCSPTPGNSVNDMPAIASLKIPAMIHEQRQKDDDAIE 480

Query: 481 SIKTVLSKVQDIWPLIQSGFLHQVLRTLRFCKEALGTFTHGSDTSSGALAFTLQYLKIIK 540
           SIKT+L KVQDIWPLIQSG LH+ LRTLRFCKE LG FT+ +D  SGALAFTLQYLKI+K
Sbjct: 481 SIKTILLKVQDIWPLIQSGGLHEALRTLRFCKETLGIFTYRTDRYSGALAFTLQYLKIMK 540

Query: 541 LVAKVWNLMSAKLNCLRKIGEWELLLGKLERGLKELRSRFIGFSKEEELHILELMLVTYT 600
           L+A+VW LMS+K +C R+IGEW  LLGKLER LKELRSRFIGFSKEEE HILELMLVT T
Sbjct: 541 LIAEVWKLMSSKHSCPRRIGEWGFLLGKLERRLKELRSRFIGFSKEEERHILELMLVTCT 600

Query: 601 LRLYNGEVWCHLMTLRKLSIIASDIEHLLKEGSFEPSTFVFEAQKALSSLGTVTPKASYN 660
           LRL +GEV CHL  LRKLS IA++IEHLLKE   EPSTFV E Q++LS+LG +TPKA  +
Sbjct: 601 LRLSSGEVCCHLTILRKLSTIATNIEHLLKEECKEPSTFVCEVQRSLSNLGRITPKAHCS 660

Query: 661 LFDFRKLLQSFTLNHLEFSGKLKHIKAELVILDNDYEKPLHFVPGLPVGIPCQIILHNVL 720
             DFRKLL+SFTLNHLE S  L+H+KAELV+LDNDYEKPL+FVPGLPVGIPCQIILHNV 
Sbjct: 661 SPDFRKLLKSFTLNHLEISENLEHVKAELVVLDNDYEKPLYFVPGLPVGIPCQIILHNVR 720

Query: 721 SERKLWVRISMDGMTSQFVFLDFPVFGGCDEVREFTYVVPFYRTPKASSFIARICIGLEC 780
           S+RKLW RI+MD MTSQF+FLDF   GGCDEVREFTY+VPFYRTPKASSFIARICIGLEC
Sbjct: 721 SKRKLWFRITMDNMTSQFIFLDFLSLGGCDEVREFTYIVPFYRTPKASSFIARICIGLEC 780

Query: 781 CFESAEVCKRHRHGGPRRDLAYICKEKEVYLSMINKG 816
            FE+ EV    R GGP+RDLAYICKEKEVY SMI+KG
Sbjct: 781 WFENTEV--NERCGGPKRDLAYICKEKEVYFSMIHKG 815

BLAST of MS016760 vs. NCBI nr
Match: XP_023527352.1 (protein SIEL isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1296.6 bits (3354), Expect = 0.0e+00
Identity = 666/816 (81.62%), Postives = 720/816 (88.24%), Query Frame = 0

Query: 1   MAERDRELVSAIDELDDGSFLSLCFGPSVSIRTWLLNNAERFQIRPSLLFTVFLGFTKDP 60
           MAERD ELVSAI+ELDD SFLSLCFGPSVSIR WLL NAERFQIRPSLL TVFLGFTKDP
Sbjct: 1   MAERDSELVSAINELDDRSFLSLCFGPSVSIRIWLLINAERFQIRPSLLLTVFLGFTKDP 60

Query: 61  YPYVRKAALDGLVGLGNNVFEDGGMIEGCYYRAIELLNDMEDCVRSAAVRVVITWGLMLA 120
           YPYVRKAALDGL GLGN V EDG MIE CY+RAIELLND+EDCVRSAAVRVVITWGLMLA
Sbjct: 61  YPYVRKAALDGLAGLGNTVVEDGSMIECCYFRAIELLNDVEDCVRSAAVRVVITWGLMLA 120

Query: 121 AHSPERKRYLSDEIFINLCSMTRDMSMKVRVNAFDAIKKLEIVSEDILLQSVSKRVLGIF 180
           AHSPERK++ SDEIF NLCSMTRDMSM+VR NAF AIK+LEIVSED+LLQS+SKRVL IF
Sbjct: 121 AHSPERKQHFSDEIFANLCSMTRDMSMEVRFNAFVAIKRLEIVSEDLLLQSMSKRVLSIF 180

Query: 181 KGKRPLVQCSTEQLEMLALDVAGAFVHGVEDEFYQVRKSACDALYNLPILSTKFAGESLN 240
           KGK+ LVQC TEQLEMLALDVAGAFVHGVEDEF+QVRKSACDAL+NL ILSTKFAGE+L+
Sbjct: 181 KGKKSLVQCYTEQLEMLALDVAGAFVHGVEDEFHQVRKSACDALFNLIILSTKFAGEALS 240

Query: 241 LLMDVLNDDSVSVRLQALETLHHMAMSKCLKLQEAHMHMFLGALSDSNGHVRSALRKLLK 300
           LLMDVLNDDSVSVRLQALETLHHMA+S CL+LQEAHMHMFL ALSD+NGHVRSALRKLLK
Sbjct: 241 LLMDVLNDDSVSVRLQALETLHHMAISNCLQLQEAHMHMFLSALSDNNGHVRSALRKLLK 300

Query: 301 LAKLPDLETFQLSFNGLLKSLESYPQDESDVLSVLFHMGQNHVNMAASIIQGVFKQIDPT 360
           LAKLPDL TFQLSFNGL++SLESYPQDESDVLSVLFHMGQNHVNM ASII  VF+QIDP 
Sbjct: 301 LAKLPDLVTFQLSFNGLVESLESYPQDESDVLSVLFHMGQNHVNMVASIITDVFEQIDPA 360

Query: 361 SEGELGFDSVKVTAYIVLAISAPVLDDHTLRIPSRLFSYAVTLLGKISHALGDIMDQSTF 420
           SEG+LGFDSVKV AY VLAISAPVLD H+LRIP R+FSYA TLLG+ISHALGDIMDQST 
Sbjct: 361 SEGKLGFDSVKVIAYTVLAISAPVLDTHSLRIPPRIFSYAATLLGRISHALGDIMDQSTI 420

Query: 421 FAYLLENCKNIGSSDLVLNPEGSPCSPTPGGSINDIL--VSLRTTAMINEQQHKHDDTIE 480
           FAYLL+N KN G SDL  NPEG PCS TPG  +NDIL   S +T A I+E+QHK DD IE
Sbjct: 421 FAYLLQNSKNTGLSDLGFNPEGVPCSLTPGSYVNDILAIASPKTPATIHEKQHKDDDAIE 480

Query: 481 SIKTVLSKVQDIWPLIQSGFLHQVLRTLRFCKEALGTFTHGSDTSSGALAFTLQYLKIIK 540
           SIKT+LSKVQDIWPLIQSGFLH+VLRTLR CKEAL  FT+  D  SGALAFTLQYLKI+K
Sbjct: 481 SIKTILSKVQDIWPLIQSGFLHEVLRTLRVCKEALEVFTYQIDKYSGALAFTLQYLKIMK 540

Query: 541 LVAKVWNLMSAKLNCLRKIGEWELLLGKLERGLKELRSRFIGFSKEEELHILELMLVTYT 600
           LVAKVWNLMS+K +C  +IGEWE LLGKLE+GLK LRSRFIGFSKEEE HILELMLVT  
Sbjct: 541 LVAKVWNLMSSKHSC--RIGEWESLLGKLEKGLKGLRSRFIGFSKEEERHILELMLVTCA 600

Query: 601 LRLYNGEVWCHLMTLRKLSIIASDIEHLLKEGSFEPSTFVFEAQKALSSLGTVTPKASYN 660
           L+L NGE+ CHL  +RKLS+IAS+IEHLLKE   EPSTFV E Q++LS LG +TPKAS  
Sbjct: 601 LKLSNGEICCHLTIMRKLSMIASNIEHLLKEECIEPSTFVCEVQRSLSKLGAITPKASCY 660

Query: 661 LFDFRKLLQSFTLNHLEFSGKLKHIKAELVILDNDYEKPLHFVPGLPVGIPCQIILHNVL 720
             DFRKLL++FTLNHLE S KLKH+KAELVI DNDYEKPL+FVPGLPVGI CQIILHNV 
Sbjct: 661 SLDFRKLLKTFTLNHLEISEKLKHVKAELVIPDNDYEKPLYFVPGLPVGILCQIILHNVP 720

Query: 721 SERKLWVRISMDGMTSQFVFLDF-PVFGGCDEVREFTYVVPFYRTPKASSFIARICIGLE 780
           SERKLW RI+MD  TSQF+FLDF  + GGCDEVREFTY VPFYRTPKASSFIARICIGLE
Sbjct: 721 SERKLWFRITMDNTTSQFIFLDFLSLGGGCDEVREFTYTVPFYRTPKASSFIARICIGLE 780

Query: 781 CCFESAEVCKRHRHGGPRRDLAYICKEKEVYLSMIN 814
           C FESAEV    R GGP+RDLAYICKEKEVYLSMI+
Sbjct: 781 CWFESAEV--NERRGGPKRDLAYICKEKEVYLSMIH 812

BLAST of MS016760 vs. NCBI nr
Match: KAG7017202.1 (Protein SIEL [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1293.9 bits (3347), Expect = 0.0e+00
Identity = 665/816 (81.50%), Postives = 718/816 (87.99%), Query Frame = 0

Query: 1   MAERDRELVSAIDELDDGSFLSLCFGPSVSIRTWLLNNAERFQIRPSLLFTVFLGFTKDP 60
           MAERD ELVSAI+ELDD SFLSLCFGPSVSIR WLL NAERFQIRPSLL TVFLGFTKDP
Sbjct: 1   MAERDSELVSAINELDDRSFLSLCFGPSVSIRIWLLKNAERFQIRPSLLLTVFLGFTKDP 60

Query: 61  YPYVRKAALDGLVGLGNNVFEDGGMIEGCYYRAIELLNDMEDCVRSAAVRVVITWGLMLA 120
           YPYVRKAALDGL GLGN V EDG MIE CY+RAIELLND+EDCVRSAAVRVVITWGLMLA
Sbjct: 61  YPYVRKAALDGLAGLGNTVVEDGSMIECCYFRAIELLNDVEDCVRSAAVRVVITWGLMLA 120

Query: 121 AHSPERKRYLSDEIFINLCSMTRDMSMKVRVNAFDAIKKLEIVSEDILLQSVSKRVLGIF 180
           AHSPERK++ SDEIF NLCSMTRDMSM+VR NAF AIK+LEIVSED+LLQS+SKRVL IF
Sbjct: 121 AHSPERKQHFSDEIFANLCSMTRDMSMEVRFNAFVAIKRLEIVSEDLLLQSMSKRVLSIF 180

Query: 181 KGKRPLVQCSTEQLEMLALDVAGAFVHGVEDEFYQVRKSACDALYNLPILSTKFAGESLN 240
           KGK+ LVQC TEQLEMLALDVAGAFVHGVEDEF+QVRKSACDAL+NL ILSTKFAGE+L+
Sbjct: 181 KGKKSLVQCYTEQLEMLALDVAGAFVHGVEDEFHQVRKSACDALFNLIILSTKFAGEALS 240

Query: 241 LLMDVLNDDSVSVRLQALETLHHMAMSKCLKLQEAHMHMFLGALSDSNGHVRSALRKLLK 300
           LLMDVLNDDSVSVRLQALETLHHMA+S CL+LQEAHMHMFL ALSD+NGHVRSALRKLLK
Sbjct: 241 LLMDVLNDDSVSVRLQALETLHHMAISNCLQLQEAHMHMFLSALSDNNGHVRSALRKLLK 300

Query: 301 LAKLPDLETFQLSFNGLLKSLESYPQDESDVLSVLFHMGQNHVNMAASIIQGVFKQIDPT 360
           LAKLPDL TFQLSFNGL++SLESYPQDESDVLSVLFHMGQNHVNM ASII  VF+QIDP 
Sbjct: 301 LAKLPDLVTFQLSFNGLVESLESYPQDESDVLSVLFHMGQNHVNMVASIITDVFEQIDPA 360

Query: 361 SEGELGFDSVKVTAYIVLAISAPVLDDHTLRIPSRLFSYAVTLLGKISHALGDIMDQSTF 420
           SEG+LGFDSVKV AYIVLAISAPVLD H+LRIP R+FSYA TLLG+ISHAL DIMDQST 
Sbjct: 361 SEGKLGFDSVKVIAYIVLAISAPVLDTHSLRIPPRIFSYAATLLGRISHALSDIMDQSTI 420

Query: 421 FAYLLENCKNIGSSDLVLNPEGSPCSPTPGGSINDIL--VSLRTTAMINEQQHKHDDTIE 480
           FAYLL+N KN G SDL  NPEG PCS TPG  +NDIL   S +T A I+E+QHK DD IE
Sbjct: 421 FAYLLQNSKNTGLSDLGFNPEGVPCSLTPGSYVNDILAIASPKTPATIHEKQHKDDDAIE 480

Query: 481 SIKTVLSKVQDIWPLIQSGFLHQVLRTLRFCKEALGTFTHGSDTSSGALAFTLQYLKIIK 540
           SIKT+LSKVQDIWPLIQSGFLH+VLRTLR CKEAL  FT+  D   GALAFTLQYLKI+K
Sbjct: 481 SIKTILSKVQDIWPLIQSGFLHEVLRTLRVCKEALEVFTYQIDKYGGALAFTLQYLKIMK 540

Query: 541 LVAKVWNLMSAKLNCLRKIGEWELLLGKLERGLKELRSRFIGFSKEEELHILELMLVTYT 600
           LVAKVWNLMS+K +C  +IGEWE LLGKLE+GLK LRSRFIGFSKEEE HILELMLVT  
Sbjct: 541 LVAKVWNLMSSKHSC--RIGEWESLLGKLEKGLKGLRSRFIGFSKEEERHILELMLVTSA 600

Query: 601 LRLYNGEVWCHLMTLRKLSIIASDIEHLLKEGSFEPSTFVFEAQKALSSLGTVTPKASYN 660
           LRL NGE+ CHL  +RKLS+IAS+IEHLLKE   EPSTFV E Q++LS LG +TPKAS  
Sbjct: 601 LRLSNGEICCHLTIMRKLSMIASNIEHLLKEECIEPSTFVCEVQRSLSKLGAITPKASCY 660

Query: 661 LFDFRKLLQSFTLNHLEFSGKLKHIKAELVILDNDYEKPLHFVPGLPVGIPCQIILHNVL 720
             DFRKLL++FTLNHLE S KLKH+KAELVI DNDYEK L+FVPGLPVGI CQIILHNV 
Sbjct: 661 SLDFRKLLKTFTLNHLEISEKLKHVKAELVIPDNDYEKTLYFVPGLPVGILCQIILHNVP 720

Query: 721 SERKLWVRISMDGMTSQFVFLDF-PVFGGCDEVREFTYVVPFYRTPKASSFIARICIGLE 780
           SERKLW RI+MD  TSQF+FLDF  + GGCDEVREFTY VPFYRTPKASSFIARICIGLE
Sbjct: 721 SERKLWFRITMDNTTSQFIFLDFLSLGGGCDEVREFTYTVPFYRTPKASSFIARICIGLE 780

Query: 781 CCFESAEVCKRHRHGGPRRDLAYICKEKEVYLSMIN 814
           C FESAEV    R GGP+RDLAYICKEKEVYLSMI+
Sbjct: 781 CWFESAEV--NERRGGPKRDLAYICKEKEVYLSMIH 812

BLAST of MS016760 vs. ExPASy Swiss-Prot
Match: Q8VZA0 (Protein SIEL OS=Arabidopsis thaliana OX=3702 GN=SIEL PE=1 SV=1)

HSP 1 Score: 590.9 bits (1522), Expect = 2.2e-167
Identity = 339/843 (40.21%), Postives = 493/843 (58.48%), Query Frame = 0

Query: 1   MAERDRELVSAIDELDDGSFLSLCFGPSVSIRTWLLNNAERFQIRPSLLFTVFLGFTKDP 60
           ++ER   + +A+ ++DD  F S+C G  +S R WLL NA+RF +  S+LFT+FLGF+KDP
Sbjct: 112 LSERTPSIAAALSKIDDEVFASICLGAPISSRLWLLRNADRFNVPSSVLFTLFLGFSKDP 171

Query: 61  YPYVRKAALDGLVGLGN-NVFEDGGMIEGCYYRAIELLNDMEDCVRSAAVRVVITWGLML 120
           YPY+RK ALDGL+ + N   F     +EGCY RA+ELL+D ED VRS+AVR V  WG ++
Sbjct: 172 YPYIRKVALDGLINICNAGDFNHTHAVEGCYTRAVELLSDAEDSVRSSAVRAVSVWGKVM 231

Query: 121 AAHSPE--RKRYLSDEIFINLCSMTRDMSMKVRVNAFDAIKKLEIVSEDILLQSVSKRVL 180
            A   E   +R  +D +F+ LCS+ RDMS+ VRV  F A   +   SE I+LQ++SK+VL
Sbjct: 232 IASKEEEMNRRDCTDAVFLQLCSVVRDMSVDVRVEVFKAFGIIGTASESIILQTLSKKVL 291

Query: 181 GIFKGKRPLVQCSTEQLEMLALDVAGAFVHGVEDEFYQVRKSACDALYNLPILSTKFAGE 240
           G  KGK+P    S    ++ +   AG ++HG EDEFY+VR++A D+ ++L + S KF  E
Sbjct: 292 GAGKGKKPQNLLSNGSADVSS--AAGVYIHGFEDEFYEVREAAVDSFHSLSVNSIKFPDE 351

Query: 241 SLNLLMDVLNDDSVSVRLQALETLHHMAMSKCLKLQEAHMHMFLGALSDSNGHVRSALRK 300
           ++ LLMD+L DD + VRL+AL+ LHH+A    LK+QE +M  FL A+ D++ ++R   R 
Sbjct: 352 AVYLLMDMLYDDYMVVRLKALKALHHIADLGNLKIQETYMPAFLDAIVDTSENIRVEARN 411

Query: 301 LLKLAKLPDLETFQLSFNGLLKSLESYPQDESDVLSVLFHMGQNHVNMAASIIQGVFKQI 360
           +LKLAKLPDL+      +G+LKSLE YPQDE D+LS LFH GQNH N   S+++   +++
Sbjct: 412 ILKLAKLPDLKLVNKCIDGVLKSLEMYPQDEPDILSALFHFGQNHTNFLVSMVKRFSEKL 471

Query: 361 DPTSEGELGFDSVKVTAYIVLAISAPVLDDHTL-RIPSRLFSYAVTLLGKISHALGDIMD 420
              S  +  F+S +++A + L ISAP+ +  ++  IP   FSY++ +LGK S  L D+MD
Sbjct: 472 GTASGSKAEFNSRQLSASLTLIISAPLSNKQSITSIPPLAFSYSLAMLGKFSSGLHDMMD 531

Query: 421 QSTFFAYLLENCKNIGSS--------------------DLVLNPEGSPCSPTPGGSINDI 480
           Q    AY L +C  + SS                    DL  NP   P    P  S    
Sbjct: 532 QDMLLAY-LTHCAILSSSSGTEFNKGDVFFHAYRDSNADLAGNPVLLPGKDIPAES---K 591

Query: 481 LVSLRTTAMINEQQHKHDDTIESIKTVLSKVQDIWPLIQSGFLHQVLRTLRFCKEALGTF 540
            ++ +    I  Q       ++ +  +L K++  W L QSG   + LR LR CK+ L T 
Sbjct: 592 YMACKAELEIGNQ------ALKFVNHILLKIKAAWLLSQSGCSKEALRALRACKQELATL 651

Query: 541 THGSDTSSGALAFTLQYLKIIKLVAKVWNLMSAKLNCLRKIG-----EWELLLGKLERGL 600
           T  S  S G L F  QY+ +I+L+ +VW       N  R I      E ELL+ ++E  L
Sbjct: 652 TADSSISKGTLDFICQYVHVIELLVQVW----PHFNYSRHISTCSSVEVELLMEEVEIKL 711

Query: 601 KELRSRFIGFSKEEELHILELMLVTYTLRLYNGEVWCHLMTLRKLSIIASDIEHLLKEGS 660
            E+R RF G S EE L +LEL++    LRLY  E+ C L  + KLS   S +E   ++  
Sbjct: 712 MEIRCRFTGLSTEESL-VLELVIFGCLLRLYKFEICCRLSCMEKLSSTISQLELHHEQQC 771

Query: 661 FEPSTFVFEAQKALSSLGTVTPKASYNLFDFRKLLQSFTLNHLEFSGKLKHIKAELVILD 720
            +PS F+ E +K+L   G+     S  L D  K+ + F+     FS  L+ + AE+ +  
Sbjct: 772 TKPSDFLTETKKSLEEFGSSDDINSCRLLDLIKIFKCFSPEQFTFSVNLQCVSAEVEVPG 831

Query: 721 NDYEKPLHFVPGLPVGIPCQIILHNVLSERKLWVRISMDGMTSQFVFLDFPVFGGCDEVR 780
           N    P+ FVPGLPV IPC+I L NV  +  LW+RIS +  T QFV+LD  ++ G    +
Sbjct: 832 NGPYSPISFVPGLPVAIPCEITLLNVPRDTCLWLRISRNDETCQFVYLDPNLYNGNGREK 891

Query: 781 EFTYVVPFYRTPKASSFIARICIGLECCFESAEVCKRHRHGGPRRDLAYICKEKEVYLSM 815
            F +    Y TP+A  F  R+ IG+EC FE  ++C R +  GP+  +AY+CKE+E++LS+
Sbjct: 892 RFMFTAVTYMTPRAVVFTLRVSIGIECLFE--DICYRKQRHGPKHPVAYLCKEREIHLSL 935

BLAST of MS016760 vs. ExPASy Swiss-Prot
Match: Q8CIM8 (Integrator complex subunit 4 OS=Mus musculus OX=10090 GN=Ints4 PE=1 SV=1)

HSP 1 Score: 131.3 bits (329), Expect = 4.8e-29
Identity = 105/373 (28.15%), Postives = 167/373 (44.77%), Query Frame = 0

Query: 59  DPYPYVRKAALDGLVGLGNNVFEDGGMIEGCYYRAIELLNDMEDCVRSAAVRVVITWGL- 118
           D  P VR AA+  ++ L     +   + +  Y +A +LL+D  + VRSAAV+++  W + 
Sbjct: 207 DQDPRVRTAAIKAMLQLHERGLK---LHQTIYNQACKLLSDDYEQVRSAAVQLI--WVVS 266

Query: 119 ------MLAAHSPERKRYLSDEIFINLCSMTRDMSMKVRVNAFDAIKKLEIVSEDILLQS 178
                 ++   S   +  L D+ F  +C M  D S  VRV A   +  +E VS   L Q+
Sbjct: 267 QLYPESIVPIPSSNEEIRLVDDAFGKICHMVSDGSWVVRVQAAKLLGSMEQVSSHFLEQT 326

Query: 179 VSKRVLGIFKGKR-------------------------PLVQCSTEQLEMLALDVAGAFV 238
           + K+++   + KR                         P  +  T  + ++     GAFV
Sbjct: 327 LDKKLMSDLRRKRTAHERAKELYSSGEFSSGRKWGDDAPKEEIDTGAVNLIESGACGAFV 386

Query: 239 HGVEDEFYQVRKSACDALYNLPILSTKFAGESLNLLMDVLNDDSVSVRLQALETLHHMAM 298
           HG+EDE Y+VR +A +AL  L   S  FA + L+ L+D+ ND+   VRLQ++ T+    +
Sbjct: 387 HGLEDEMYEVRIAAVEALCMLAQSSPSFAEKCLDFLVDMFNDEIEEVRLQSIHTMR--KI 446

Query: 299 SKCLKLQEAHMHMFLGALSDSNGHVRSALRKLLKLAKLPDLETFQLSFNGLLKSLESYPQ 358
           S  + L+E  +   L  L DS+  +R AL +LL    +   E   L+   LLK+L  YP 
Sbjct: 447 SNNITLREDQLDTVLAVLEDSSRDIREALHELLCCTNVSTKEGIHLALVELLKNLTKYPT 506

Query: 359 DESDVLSVLFHMGQNHVNMAASIIQGVFKQIDPTSEGELGFDSVKVTAYIVLAISA---- 392
           D   +   L  +G  H  +   ++  +          E   D     A +VL  +A    
Sbjct: 507 DRDSIWKCLKFLGSRHPTLVLPLVPELLSTHPFFDTAEPDMDDPAYIAVLVLIFNAAKTC 566

BLAST of MS016760 vs. ExPASy Swiss-Prot
Match: Q96HW7 (Integrator complex subunit 4 OS=Homo sapiens OX=9606 GN=INTS4 PE=1 SV=2)

HSP 1 Score: 128.6 bits (322), Expect = 3.1e-28
Identity = 161/711 (22.64%), Postives = 275/711 (38.68%), Query Frame = 0

Query: 56  FTKDPYPYVRKAALDGLVGLGNNVFEDGGMIEGCYYRAIELLNDMEDCVRSAAVRVVITW 115
           +  D  P VR AA+  ++ L     +   + +  Y +A +LL+D  + VRSAAV+++  W
Sbjct: 203 YFSDQDPRVRTAAIKAMLQLHERGLK---LHQTIYNQACKLLSDDYEQVRSAAVQLI--W 262

Query: 116 GL-------MLAAHSPERKRYLSDEIFINLCSMTRDMSMKVRVNAFDAIKKLEIVSEDIL 175
            +       ++   S   +  L D+ F  +C M  D S  VRV A   +  +E VS   L
Sbjct: 263 VVSQLYPESIVPIPSSNEEIRLVDDAFGKICHMVSDGSWVVRVQAAKLLGSMEQVSSHFL 322

Query: 176 LQSVSKRVLGIFKGKR-------------------------PLVQCSTEQLEMLALDVAG 235
            Q++ K+++   + KR                         P  +  T  + ++     G
Sbjct: 323 EQTLDKKLMSDLRRKRTAHERAKELYSSGEFSSGRKWGDDAPKEEVDTGAVNLIESGACG 382

Query: 236 AFVHGVEDEFYQVRKSACDALYNLPILSTKFAGESLNLLMDVLNDDSVSVRLQALETLHH 295
           AFVHG+EDE Y+VR +A +AL  L   S  FA + L+ L+D+ ND+   VRLQ++ T+  
Sbjct: 383 AFVHGLEDEMYEVRIAAVEALCMLAQSSPSFAEKCLDFLVDMFNDEIEEVRLQSIHTMR- 442

Query: 296 MAMSKCLKLQEAHMHMFLGALSDSNGHVRSALRKLLKLAKLPDLETFQLSFNGLLKSLES 355
             +S  + L+E  +   L  L DS+  +R AL +LL    +   E   L+   LLK+L  
Sbjct: 443 -KISNNITLREDQLDTVLAVLEDSSRDIREALHELLCCTNVSTKEGIHLALVELLKNLTK 502

Query: 356 YPQDESDVLSVLFHMGQNHVNMAASIIQGVFKQIDPTSEGELGFDSVKVTAYIVLAISA- 415
           YP D   +   L  +G  H  +   ++  +          E   D     A +VL  +A 
Sbjct: 503 YPTDRDSIWKCLKFLGSRHPTLVLPLVPELLSTHPFFDTAEPDMDDPAYIAVLVLIFNAA 562

Query: 416 -------PVLDDHTLRIPSRLFSYAVTLLGKISHALGDIMDQSTFFAYLLENCKNIGSSD 475
                   +  DHT R                             +AYL ++  ++    
Sbjct: 563 KTCPTMPALFSDHTFR----------------------------HYAYLRDSLSHL---- 622

Query: 476 LVLNPEGSPCSPTPGGSINDILVSLRTTAMINEQQHKHDDTIESIKTVLSKVQDIWPLIQ 535
                   P    PG  +    VS    ++I ++        +S++ V S +Q + P   
Sbjct: 623 -------VPALRLPGRKLVSSAVS---PSIIPQEDPSQQFLQQSLERVYS-LQHLDPQGA 682

Query: 536 SGFLHQVLRTLRFCKEALGTFTHGSDTSSGALAFTLQYLKIIKLVAKVWNLMSAKLNCLR 595
              L   +R L+   E        +D S  A     Q L I  L  K+WN+ +     L+
Sbjct: 683 QELLEFTIRDLQRLGELQSELAGVADFS--ATYLRCQLLLIKALQEKLWNVAAPLY--LK 742

Query: 596 KIGEWELLLGKLERGLKELRSRFIGFSKEEELHILELMLVTYTLRLYNGEVWCHLMTLRK 655
           +         ++     ++   + G   ++ + I  + L    L+L          T R 
Sbjct: 743 QSDLASAAAKQIMEETYKMEFMYSGVENKQVVIIHHMRLQAKALQLI-----VTARTTRG 802

Query: 656 LSIIASDIEHLLKEGSFEPSTFVFEAQKALSSL---------GTVTPKASYNLFDFRKLL 715
           L  +    E  L+E  F    F+ +      S            +T K +  +   + +L
Sbjct: 803 LDPLFGMCEKFLQEVDFFQRYFIADLPHLQDSFVDKLLDLMPRLMTSKPAEVVKILQTML 854

Query: 716 QSFTLNHLEFSGKLKHIKAELVILDNDYEKPLHFVPGLPVGIPCQIILHNV 718
           +     HL    ++    A ++    + + PL F  GL V +     L +V
Sbjct: 863 RQSAFLHLPLPEQIHKASATIIEPAGESDNPLRFTSGLVVALDVDATLEHV 854

BLAST of MS016760 vs. ExPASy Swiss-Prot
Match: Q68F70 (Integrator complex subunit 4 OS=Xenopus laevis OX=8355 GN=ints4 PE=2 SV=1)

HSP 1 Score: 127.1 bits (318), Expect = 9.1e-28
Identity = 104/373 (27.88%), Postives = 164/373 (43.97%), Query Frame = 0

Query: 59  DPYPYVRKAALDGLVGLGNNVFEDGGMIEGCYYRAIELLNDMEDCVRSAAVRVVITWGL- 118
           D  P VR AA+  ++ L     +   + +  Y +A +LL D  + VRSAAV   ++W L 
Sbjct: 208 DQDPRVRTAAIKAMLQLHERGLK---LQQAMYNQACKLLTDDYEQVRSAAVE--LSWVLS 267

Query: 119 ------MLAAHSPERKRYLSDEIFINLCSMTRDMSMKVRVNAFDAIKKLEIVSEDILLQS 178
                 ++   S   +  L D+ F  +C M  D S  VRV A   +  +  VS   L Q+
Sbjct: 268 QLYSESIVPIPSSNEEIRLVDDAFGKVCHMVSDGSWVVRVQACKLLGSMLQVSPHFLEQT 327

Query: 179 VSKRVLGIFKGKR-------------------------PLVQCSTEQLEMLALDVAGAFV 238
           + K+++   + KR                         P  +  T  + ++     GAFV
Sbjct: 328 LDKKLMSDLRRKRTAHERAKELYSSGEFSSGRKWGDDAPKEELDTGAVNLIDSGACGAFV 387

Query: 239 HGVEDEFYQVRKSACDALYNLPILSTKFAGESLNLLMDVLNDDSVSVRLQALETLHHMAM 298
           HG+EDE Y+VR +A ++L  L   S  FA + L+ L+D+ ND+   VRLQ++ T+    +
Sbjct: 388 HGLEDEMYEVRIAAVESLCLLARSSAPFAEKCLDFLVDMFNDEIEEVRLQSIHTMR--KI 447

Query: 299 SKCLKLQEAHMHMFLGALSDSNGHVRSALRKLLKLAKLPDLETFQLSFNGLLKSLESYPQ 358
           S  + L+E  +   L  L D +  +R AL +LL    +   E  QL+   LLK+L  YP 
Sbjct: 448 SDNITLREDQLDTVLAVLEDKSRDIREALHELLCCTNVSTKECIQLALVELLKNLSKYPT 507

Query: 359 DESDVLSVLFHMGQNHVNMAASIIQGVFKQIDPTSEGELGFDSVKVTAYIVLAISA---- 392
           D   +   L  +G  H  +  S++  +          E   D     A +VL  +A    
Sbjct: 508 DRESIWKCLKFLGSRHPTLVLSLVPELLSTHPFFDTPEPDMDDPAYIAVLVLIFNAAKCC 567

BLAST of MS016760 vs. ExPASy Swiss-Prot
Match: Q9W3E1 (Integrator complex subunit 4 OS=Drosophila melanogaster OX=7227 GN=IntS4 PE=1 SV=1)

HSP 1 Score: 125.6 bits (314), Expect = 2.6e-27
Identity = 132/569 (23.20%), Postives = 243/569 (42.71%), Query Frame = 0

Query: 64  VRKAALDGLVGLGNNVFEDGGMIEGCYY-RAIELLNDMEDCVRSAAVRVVITWG-----L 123
           VR  AL  L+ LG    E G  +    Y RA+E + D  +CVR  A+++V   G      
Sbjct: 268 VRAQALHALLTLG----ERGSQLPAVLYKRAVEAMKDDYECVRKEALQLVFMLGNRHPDY 327

Query: 124 MLAAHSPERKRYLSDEIFINLCSMTRDMSMKVRVNAFDAIKKLEIVSEDILLQSVSKRVL 183
           +L +   + +  + D  F  +C    D+S+++RV A + +  +  VS + L Q++ K+++
Sbjct: 328 ILPSDRQQEELRMIDAAFSKVCEALCDLSLQIRVLAAELLGGMTAVSREFLHQTLDKKLM 387

Query: 184 GIFKGKR-------------------------PLVQCSTEQLEMLALDVAGAFVHGVEDE 243
              + KR                         P      + + ++A    GA +HG+EDE
Sbjct: 388 SNLRRKRTAHERGARLVASGEWSSGKRWADDAPQEHLDAQSISIIASGACGALIHGLEDE 447

Query: 244 FYQVRKSACDALYNLPILSTKFAGESLNLLMDVLNDDSVSVRLQALETLHHMAMSKCLKL 303
           F +VR +A  ++  L +    FA  SL+ L+D+ ND+   VRL+A+ +L   A++K + L
Sbjct: 448 FLEVRTAAVASMCKLALSRPDFAVTSLDFLVDMFNDEIEDVRLKAIYSL--TAIAKHIVL 507

Query: 304 QEAHMHMFLGALSDSNGHVRSALRKLLKLAKLPDLETFQLSFNGLLKSLESYPQDESDVL 363
           +E  + + LG+L D +  VR  L  +L   ++       +    LL  L  YPQD +   
Sbjct: 508 REDQLEIMLGSLEDYSVDVREGLHLMLGACRVSTQTCLLMVVQKLLDVLAKYPQDRNSTY 567

Query: 364 SVLFHMGQNHVNMAASIIQGVFKQIDPTSEGELGFDSVKVTAYIVLAISAPVLDDHTLRI 423
           + +  +GQ H ++  ++   +   + P    E     V+  AY+ + I      +H + I
Sbjct: 568 ACMRKIGQKHPHLVMAVAVHLL-YVHPFF--ETPERDVEDPAYLCVLILVFNAAEHLVPI 627

Query: 424 PSRLFSYAVTLLGKISHALGDIMDQSTFFAYLLENCKNIGSSDLVLNPEGSPCSPTPGGS 483
                   ++LL   +H           +AYL ++  N+            P  P  G S
Sbjct: 628 --------ISLLPTATH---------RHYAYLRDSMPNL-----------VPQLPIEGAS 687

Query: 484 INDILVSLRTTAMINEQQHKHDDTIESIKTVLSKVQDIWPLIQSGFLHQVLRTLRFCKEA 543
                 S   T  I+   H+   + E ++ +LS +++I+ +       ++L+T +   + 
Sbjct: 688 ------SASATHRIDSAMHQAGSSAEYLQMILSHIEEIFTMTDERL--ELLQTAQSNLQR 747

Query: 544 LGTFTHGSDTSSGALAFTLQYLKIIKLVAKVWNLMSAKLNCLRKIGEWELLLGKLERGLK 602
           LG+   G   +S  L   L     I+ + +  +   +++            L  L R   
Sbjct: 748 LGSIDAGMYGTSNFLETFLAAQIQIEQMQRCASTQRSRVPLKES-------LAALIRNCL 784

BLAST of MS016760 vs. ExPASy TrEMBL
Match: A0A6J1DSR4 (protein SIEL isoform X1 OS=Momordica charantia OX=3673 GN=LOC111024055 PE=4 SV=1)

HSP 1 Score: 1602.8 bits (4149), Expect = 0.0e+00
Identity = 805/815 (98.77%), Postives = 808/815 (99.14%), Query Frame = 0

Query: 1   MAERDRELVSAIDELDDGSFLSLCFGPSVSIRTWLLNNAERFQIRPSLLFTVFLGFTKDP 60
           MAERDRELVSAIDELDDGSFLSLCFGPSVSIRTWLLNNAERFQIRPSLLFTVFLGFTKDP
Sbjct: 1   MAERDRELVSAIDELDDGSFLSLCFGPSVSIRTWLLNNAERFQIRPSLLFTVFLGFTKDP 60

Query: 61  YPYVRKAALDGLVGLGNNVFEDGGMIEGCYYRAIELLNDMEDCVRSAAVRVVITWGLMLA 120
           YPYVR AALDGLVGLGNNVFEDGGMIEGCYYRAIELLNDMEDCVRSAAVRVVITWGLMLA
Sbjct: 61  YPYVRIAALDGLVGLGNNVFEDGGMIEGCYYRAIELLNDMEDCVRSAAVRVVITWGLMLA 120

Query: 121 AHSPERKRYLSDEIFINLCSMTRDMSMKVRVNAFDAIKKLEIVSEDILLQSVSKRVLGIF 180
           AHSPERKRYLSDEIFINLCSMTRDMSMKVRVNAFDAIKKLEIVSEDILLQSVSK+VLGIF
Sbjct: 121 AHSPERKRYLSDEIFINLCSMTRDMSMKVRVNAFDAIKKLEIVSEDILLQSVSKKVLGIF 180

Query: 181 KGKRPLVQCSTEQLEMLALDVAGAFVHGVEDEFYQVRKSACDALYNLPILSTKFAGESLN 240
           KGK+PLVQCSTEQLEMLALDVAGAFVHGVEDEFYQVRKSACDALYNLPILSTKFAGE+LN
Sbjct: 181 KGKKPLVQCSTEQLEMLALDVAGAFVHGVEDEFYQVRKSACDALYNLPILSTKFAGEALN 240

Query: 241 LLMDVLNDDSVSVRLQALETLHHMAMSKCLKLQEAHMHMFLGALSDSNGHVRSALRKLLK 300
           LLMDVLNDDSVSVRLQALETLHHMAMSKCLKLQEAHMHMFLGALSDSNGHVRSALRKLLK
Sbjct: 241 LLMDVLNDDSVSVRLQALETLHHMAMSKCLKLQEAHMHMFLGALSDSNGHVRSALRKLLK 300

Query: 301 LAKLPDLETFQLSFNGLLKSLESYPQDESDVLSVLFHMGQNHVNMAASIIQGVFKQIDPT 360
           L KLPDLETFQLSFNGLLKSLESY QDES VLSVLFHMGQNHVNMAASIIQGVFKQIDPT
Sbjct: 301 LVKLPDLETFQLSFNGLLKSLESYAQDESGVLSVLFHMGQNHVNMAASIIQGVFKQIDPT 360

Query: 361 SEGELGFDSVKVTAYIVLAISAPVLDDHTLRIPSRLFSYAVTLLGKISHALGDIMDQSTF 420
           SEGELGFDSVKVTAYIVLAISAPVLDDHTLRIPSRLFSYAVTLLGKISHALGDIMDQSTF
Sbjct: 361 SEGELGFDSVKVTAYIVLAISAPVLDDHTLRIPSRLFSYAVTLLGKISHALGDIMDQSTF 420

Query: 421 FAYLLENCKNIGSSDLVLNPEGSPCSPTPGGSINDILVSLRTTAMINEQQHKHDDTIESI 480
           FAYLLENCKNIGSSDLVLNPEGSPCSPTPGGSINDILVSLRTTAMINEQQHKHDDTIESI
Sbjct: 421 FAYLLENCKNIGSSDLVLNPEGSPCSPTPGGSINDILVSLRTTAMINEQQHKHDDTIESI 480

Query: 481 KTVLSKVQDIWPLIQSGFLHQVLRTLRFCKEALGTFTHGSDTSSGALAFTLQYLKIIKLV 540
           KTVLSKVQDIWPLIQSGFLHQVLRTLRFCKEALGTFTHGSDTSSGALAFTLQYLKIIKLV
Sbjct: 481 KTVLSKVQDIWPLIQSGFLHQVLRTLRFCKEALGTFTHGSDTSSGALAFTLQYLKIIKLV 540

Query: 541 AKVWNLMSAKLNCLRKIGEWELLLGKLERGLKELRSRFIGFSKEEELHILELMLVTYTLR 600
           AKVWNLMSAKLNCLRKIGEWELLLGKLERGLKELRSRFIGFSKEEELHILELMLVTYTLR
Sbjct: 541 AKVWNLMSAKLNCLRKIGEWELLLGKLERGLKELRSRFIGFSKEEELHILELMLVTYTLR 600

Query: 601 LYNGEVWCHLMTLRKLSIIASDIEHLLKEGSFEPSTFVFEAQKALSSLGTVTPKASYNLF 660
           LYNGEVWCHLMTLRKLSIIASDIEHLLKEGSFEPSTFVFEAQKALSSLGTVTPKASYNLF
Sbjct: 601 LYNGEVWCHLMTLRKLSIIASDIEHLLKEGSFEPSTFVFEAQKALSSLGTVTPKASYNLF 660

Query: 661 DFRKLLQSFTLNHLEFSGKLKHIKAELVILDNDYEKPLHFVPGLPVGIPCQIILHNVLSE 720
           DFRKLLQSFTLNHLEFSGKLKHIKAEL ILDNDYEKPLHFVPGLPVGIPCQIILHNVLSE
Sbjct: 661 DFRKLLQSFTLNHLEFSGKLKHIKAELFILDNDYEKPLHFVPGLPVGIPCQIILHNVLSE 720

Query: 721 RKLWVRISMDGMTSQFVFLDFPVFGGCDEVREFTYVVPFYRTPKASSFIARICIGLECCF 780
           RKLWVRISMDGMTSQFVFLDFPVFGGCDEVREFTYVVPFYRTPKASSFIARICIGLECCF
Sbjct: 721 RKLWVRISMDGMTSQFVFLDFPVFGGCDEVREFTYVVPFYRTPKASSFIARICIGLECCF 780

Query: 781 ESAEVCKRHRHGGPRRDLAYICKEKEVYLSMINKG 816
           ESAEV KRH HGGPRRDLAYICKEKEVYLSMINKG
Sbjct: 781 ESAEVSKRHGHGGPRRDLAYICKEKEVYLSMINKG 815

BLAST of MS016760 vs. ExPASy TrEMBL
Match: A0A6J1DXR0 (protein SIEL isoform X2 OS=Momordica charantia OX=3673 GN=LOC111024055 PE=4 SV=1)

HSP 1 Score: 1366.3 bits (3535), Expect = 0.0e+00
Identity = 689/698 (98.71%), Postives = 692/698 (99.14%), Query Frame = 0

Query: 118 MLAAHSPERKRYLSDEIFINLCSMTRDMSMKVRVNAFDAIKKLEIVSEDILLQSVSKRVL 177
           MLAAHSPERKRYLSDEIFINLCSMTRDMSMKVRVNAFDAIKKLEIVSEDILLQSVSK+VL
Sbjct: 1   MLAAHSPERKRYLSDEIFINLCSMTRDMSMKVRVNAFDAIKKLEIVSEDILLQSVSKKVL 60

Query: 178 GIFKGKRPLVQCSTEQLEMLALDVAGAFVHGVEDEFYQVRKSACDALYNLPILSTKFAGE 237
           GIFKGK+PLVQCSTEQLEMLALDVAGAFVHGVEDEFYQVRKSACDALYNLPILSTKFAGE
Sbjct: 61  GIFKGKKPLVQCSTEQLEMLALDVAGAFVHGVEDEFYQVRKSACDALYNLPILSTKFAGE 120

Query: 238 SLNLLMDVLNDDSVSVRLQALETLHHMAMSKCLKLQEAHMHMFLGALSDSNGHVRSALRK 297
           +LNLLMDVLNDDSVSVRLQALETLHHMAMSKCLKLQEAHMHMFLGALSDSNGHVRSALRK
Sbjct: 121 ALNLLMDVLNDDSVSVRLQALETLHHMAMSKCLKLQEAHMHMFLGALSDSNGHVRSALRK 180

Query: 298 LLKLAKLPDLETFQLSFNGLLKSLESYPQDESDVLSVLFHMGQNHVNMAASIIQGVFKQI 357
           LLKL KLPDLETFQLSFNGLLKSLESY QDES VLSVLFHMGQNHVNMAASIIQGVFKQI
Sbjct: 181 LLKLVKLPDLETFQLSFNGLLKSLESYAQDESGVLSVLFHMGQNHVNMAASIIQGVFKQI 240

Query: 358 DPTSEGELGFDSVKVTAYIVLAISAPVLDDHTLRIPSRLFSYAVTLLGKISHALGDIMDQ 417
           DPTSEGELGFDSVKVTAYIVLAISAPVLDDHTLRIPSRLFSYAVTLLGKISHALGDIMDQ
Sbjct: 241 DPTSEGELGFDSVKVTAYIVLAISAPVLDDHTLRIPSRLFSYAVTLLGKISHALGDIMDQ 300

Query: 418 STFFAYLLENCKNIGSSDLVLNPEGSPCSPTPGGSINDILVSLRTTAMINEQQHKHDDTI 477
           STFFAYLLENCKNIGSSDLVLNPEGSPCSPTPGGSINDILVSLRTTAMINEQQHKHDDTI
Sbjct: 301 STFFAYLLENCKNIGSSDLVLNPEGSPCSPTPGGSINDILVSLRTTAMINEQQHKHDDTI 360

Query: 478 ESIKTVLSKVQDIWPLIQSGFLHQVLRTLRFCKEALGTFTHGSDTSSGALAFTLQYLKII 537
           ESIKTVLSKVQDIWPLIQSGFLHQVLRTLRFCKEALGTFTHGSDTSSGALAFTLQYLKII
Sbjct: 361 ESIKTVLSKVQDIWPLIQSGFLHQVLRTLRFCKEALGTFTHGSDTSSGALAFTLQYLKII 420

Query: 538 KLVAKVWNLMSAKLNCLRKIGEWELLLGKLERGLKELRSRFIGFSKEEELHILELMLVTY 597
           KLVAKVWNLMSAKLNCLRKIGEWELLLGKLERGLKELRSRFIGFSKEEELHILELMLVTY
Sbjct: 421 KLVAKVWNLMSAKLNCLRKIGEWELLLGKLERGLKELRSRFIGFSKEEELHILELMLVTY 480

Query: 598 TLRLYNGEVWCHLMTLRKLSIIASDIEHLLKEGSFEPSTFVFEAQKALSSLGTVTPKASY 657
           TLRLYNGEVWCHLMTLRKLSIIASDIEHLLKEGSFEPSTFVFEAQKALSSLGTVTPKASY
Sbjct: 481 TLRLYNGEVWCHLMTLRKLSIIASDIEHLLKEGSFEPSTFVFEAQKALSSLGTVTPKASY 540

Query: 658 NLFDFRKLLQSFTLNHLEFSGKLKHIKAELVILDNDYEKPLHFVPGLPVGIPCQIILHNV 717
           NLFDFRKLLQSFTLNHLEFSGKLKHIKAEL ILDNDYEKPLHFVPGLPVGIPCQIILHNV
Sbjct: 541 NLFDFRKLLQSFTLNHLEFSGKLKHIKAELFILDNDYEKPLHFVPGLPVGIPCQIILHNV 600

Query: 718 LSERKLWVRISMDGMTSQFVFLDFPVFGGCDEVREFTYVVPFYRTPKASSFIARICIGLE 777
           LSERKLWVRISMDGMTSQFVFLDFPVFGGCDEVREFTYVVPFYRTPKASSFIARICIGLE
Sbjct: 601 LSERKLWVRISMDGMTSQFVFLDFPVFGGCDEVREFTYVVPFYRTPKASSFIARICIGLE 660

Query: 778 CCFESAEVCKRHRHGGPRRDLAYICKEKEVYLSMINKG 816
           CCFESAEV KRH HGGPRRDLAYICKEKEVYLSMINKG
Sbjct: 661 CCFESAEVSKRHGHGGPRRDLAYICKEKEVYLSMINKG 698

BLAST of MS016760 vs. ExPASy TrEMBL
Match: A0A6J1F7A9 (protein SIEL OS=Cucurbita moschata OX=3662 GN=LOC111441512 PE=4 SV=1)

HSP 1 Score: 1290.8 bits (3339), Expect = 0.0e+00
Identity = 663/816 (81.25%), Postives = 717/816 (87.87%), Query Frame = 0

Query: 1   MAERDRELVSAIDELDDGSFLSLCFGPSVSIRTWLLNNAERFQIRPSLLFTVFLGFTKDP 60
           MAERD ELVSAI+ELDD SFLSLCFGPSVSIR WLL NAERFQIRPSLL TVFLGFTKDP
Sbjct: 1   MAERDSELVSAINELDDRSFLSLCFGPSVSIRIWLLKNAERFQIRPSLLLTVFLGFTKDP 60

Query: 61  YPYVRKAALDGLVGLGNNVFEDGGMIEGCYYRAIELLNDMEDCVRSAAVRVVITWGLMLA 120
           YPYVRKAALDGL GLGN V EDG MIE CY+RAIELLND+EDCVRSAAVRVVITWGLMLA
Sbjct: 61  YPYVRKAALDGLAGLGNTVVEDGSMIECCYFRAIELLNDVEDCVRSAAVRVVITWGLMLA 120

Query: 121 AHSPERKRYLSDEIFINLCSMTRDMSMKVRVNAFDAIKKLEIVSEDILLQSVSKRVLGIF 180
           AH PERK++ SDEIF NLCSMTRDMSM+VR NAF AIK+LEIVSED+LLQS+SKRVL IF
Sbjct: 121 AHCPERKQHFSDEIFANLCSMTRDMSMEVRFNAFVAIKRLEIVSEDLLLQSMSKRVLSIF 180

Query: 181 KGKRPLVQCSTEQLEMLALDVAGAFVHGVEDEFYQVRKSACDALYNLPILSTKFAGESLN 240
           KGK+ LVQC TEQLEMLALDVAGAFVHGVEDEF+QVRKSACDAL+NL ILSTKFAGE+L+
Sbjct: 181 KGKKSLVQCYTEQLEMLALDVAGAFVHGVEDEFHQVRKSACDALFNLIILSTKFAGEALS 240

Query: 241 LLMDVLNDDSVSVRLQALETLHHMAMSKCLKLQEAHMHMFLGALSDSNGHVRSALRKLLK 300
           LLMDVLNDDSVSVRLQALETLHHMA+S CL+LQEAHMHMFL ALSD+NGHVRSALRKLLK
Sbjct: 241 LLMDVLNDDSVSVRLQALETLHHMAISNCLQLQEAHMHMFLSALSDNNGHVRSALRKLLK 300

Query: 301 LAKLPDLETFQLSFNGLLKSLESYPQDESDVLSVLFHMGQNHVNMAASIIQGVFKQIDPT 360
           LAKLPDL TFQLSFNGL++SLESYPQDESDVLSVLFHMGQNHVNM ASII  VF+QIDP 
Sbjct: 301 LAKLPDLVTFQLSFNGLVESLESYPQDESDVLSVLFHMGQNHVNMVASIITDVFEQIDPA 360

Query: 361 SEGELGFDSVKVTAYIVLAISAPVLDDHTLRIPSRLFSYAVTLLGKISHALGDIMDQSTF 420
           SEG+LGFDSVKV AYIVLAISAPVLD H+LRIP R+FSYA TLLG+ISHAL DIMDQST 
Sbjct: 361 SEGKLGFDSVKVIAYIVLAISAPVLDTHSLRIPPRIFSYAATLLGRISHALSDIMDQSTI 420

Query: 421 FAYLLENCKNIGSSDLVLNPEGSPCSPTPGGSINDIL--VSLRTTAMINEQQHKHDDTIE 480
           FAYLL+N KN G SDL  NPEG PCS TPG  +NDIL   S +T A I+++QHK DD IE
Sbjct: 421 FAYLLQNSKNTGLSDLGFNPEGVPCSLTPGSYVNDILAIASPKTPATIHDKQHKDDDAIE 480

Query: 481 SIKTVLSKVQDIWPLIQSGFLHQVLRTLRFCKEALGTFTHGSDTSSGALAFTLQYLKIIK 540
           SIKT+LSKVQDIWPLIQSGFLH+VLRTLR CKEAL  FT+  D   GALAFTLQYLKI+K
Sbjct: 481 SIKTILSKVQDIWPLIQSGFLHEVLRTLRVCKEALEVFTYQIDKYGGALAFTLQYLKIMK 540

Query: 541 LVAKVWNLMSAKLNCLRKIGEWELLLGKLERGLKELRSRFIGFSKEEELHILELMLVTYT 600
           LVAKVWNLMS+K +C  +IGEWE LLGKLE+GLK LRSRFIGFSKEEE HILELMLVT  
Sbjct: 541 LVAKVWNLMSSKHSC--RIGEWESLLGKLEKGLKGLRSRFIGFSKEEERHILELMLVTSA 600

Query: 601 LRLYNGEVWCHLMTLRKLSIIASDIEHLLKEGSFEPSTFVFEAQKALSSLGTVTPKASYN 660
           LRL NGE+ CHL  +RKLS+IAS+IEHLLKE   EPSTFV E Q++LS LG +TPKAS  
Sbjct: 601 LRLSNGEICCHLTIMRKLSMIASNIEHLLKEECIEPSTFVCEVQRSLSKLGAITPKASCY 660

Query: 661 LFDFRKLLQSFTLNHLEFSGKLKHIKAELVILDNDYEKPLHFVPGLPVGIPCQIILHNVL 720
             DFRKLL++FTLNHLE S KLKH+KAELVI DNDYEK L+FVPGLPVGI CQIILHNV 
Sbjct: 661 SLDFRKLLKTFTLNHLEISEKLKHVKAELVIPDNDYEKTLYFVPGLPVGILCQIILHNVP 720

Query: 721 SERKLWVRISMDGMTSQFVFLDF-PVFGGCDEVREFTYVVPFYRTPKASSFIARICIGLE 780
           SERKLW RI+MD  TSQF+FLDF  + GGCDEVREFTY VPFYRTPKASSFIARICIGLE
Sbjct: 721 SERKLWFRITMDNTTSQFIFLDFLSLGGGCDEVREFTYTVPFYRTPKASSFIARICIGLE 780

Query: 781 CCFESAEVCKRHRHGGPRRDLAYICKEKEVYLSMIN 814
           C FESAEV    R GGP+RDLAYICKEKEVYLSMI+
Sbjct: 781 CWFESAEV--NERRGGPKRDLAYICKEKEVYLSMIH 812

BLAST of MS016760 vs. ExPASy TrEMBL
Match: A0A6J1J0A1 (protein SIEL OS=Cucurbita maxima OX=3661 GN=LOC111481532 PE=4 SV=1)

HSP 1 Score: 1290.4 bits (3338), Expect = 0.0e+00
Identity = 663/816 (81.25%), Postives = 718/816 (87.99%), Query Frame = 0

Query: 1   MAERDRELVSAIDELDDGSFLSLCFGPSVSIRTWLLNNAERFQIRPSLLFTVFLGFTKDP 60
           MAERD ELVSAI+ELDD SFLSLCFGPSVSIR WLL NAE FQIRPSLL TVFLGFTKDP
Sbjct: 1   MAERDSELVSAINELDDRSFLSLCFGPSVSIRIWLLKNAESFQIRPSLLLTVFLGFTKDP 60

Query: 61  YPYVRKAALDGLVGLGNNVFEDGGMIEGCYYRAIELLNDMEDCVRSAAVRVVITWGLMLA 120
           YPYVRKAALDGL GLG  V EDG MIE CY+RAIELLND+EDCVRSAAVRVVITWGLMLA
Sbjct: 61  YPYVRKAALDGLAGLGITVVEDGSMIECCYFRAIELLNDVEDCVRSAAVRVVITWGLMLA 120

Query: 121 AHSPERKRYLSDEIFINLCSMTRDMSMKVRVNAFDAIKKLEIVSEDILLQSVSKRVLGIF 180
           AHSPERK++ SDEIF NLCSMTRDMSM+VR NAF AIK+LEIVSED+LLQS+SKRVL IF
Sbjct: 121 AHSPERKQHFSDEIFANLCSMTRDMSMEVRSNAFVAIKRLEIVSEDLLLQSMSKRVLSIF 180

Query: 181 KGKRPLVQCSTEQLEMLALDVAGAFVHGVEDEFYQVRKSACDALYNLPILSTKFAGESLN 240
           KGK+ LVQC TEQLEMLALDVAGAFVHGVEDEF+QVRKSACDAL+NL ILSTKF+GE+L+
Sbjct: 181 KGKKSLVQCYTEQLEMLALDVAGAFVHGVEDEFHQVRKSACDALFNLIILSTKFSGEALS 240

Query: 241 LLMDVLNDDSVSVRLQALETLHHMAMSKCLKLQEAHMHMFLGALSDSNGHVRSALRKLLK 300
           LLMDVLNDDSVSVRLQALETLHHMA+S CL+LQEAHMHMFL ALSD+NGHVRSALRKLLK
Sbjct: 241 LLMDVLNDDSVSVRLQALETLHHMAISNCLQLQEAHMHMFLSALSDNNGHVRSALRKLLK 300

Query: 301 LAKLPDLETFQLSFNGLLKSLESYPQDESDVLSVLFHMGQNHVNMAASIIQGVFKQIDPT 360
           LAKLPDL TFQLSFNGL++SLESYPQDESDVLSVLFHMGQNHVNM ASII  VF+QIDP 
Sbjct: 301 LAKLPDLGTFQLSFNGLVESLESYPQDESDVLSVLFHMGQNHVNMVASIITDVFEQIDPA 360

Query: 361 SEGELGFDSVKVTAYIVLAISAPVLDDHTLRIPSRLFSYAVTLLGKISHALGDIMDQSTF 420
           SEG+LGFDSVKV AYIVLAISAPVLD H+LRIP R+FSYA TLLG+ISHALGDIMDQST 
Sbjct: 361 SEGKLGFDSVKVMAYIVLAISAPVLDTHSLRIPPRIFSYAATLLGRISHALGDIMDQSTI 420

Query: 421 FAYLLENCKNIGSSDLVLNPEGSPCSPTPGGSINDIL--VSLRTTAMINEQQHKHDDTIE 480
           FAYLL+N KN G SDL  NPEG PCS TPG  +NDIL   S +T AMI+E+QHK DD IE
Sbjct: 421 FAYLLQNSKNTGLSDLGFNPEGVPCSLTPGSYVNDILAIASPKTPAMIHEKQHKDDDAIE 480

Query: 481 SIKTVLSKVQDIWPLIQSGFLHQVLRTLRFCKEALGTFTHGSDTSSGALAFTLQYLKIIK 540
           SIKT+LSKVQDIWPLIQSGFLH++LR LR  KEAL  FT+  D  SGALAFTLQYLKI+K
Sbjct: 481 SIKTILSKVQDIWPLIQSGFLHEILRNLRVFKEALEVFTYQIDKYSGALAFTLQYLKIMK 540

Query: 541 LVAKVWNLMSAKLNCLRKIGEWELLLGKLERGLKELRSRFIGFSKEEELHILELMLVTYT 600
           LVAKVWNLMS+K +C  +IGEWE LLGKLE+GLK LRSRFIGFSKEEE HILELMLVT  
Sbjct: 541 LVAKVWNLMSSKHSC--RIGEWESLLGKLEKGLKGLRSRFIGFSKEEERHILELMLVTSA 600

Query: 601 LRLYNGEVWCHLMTLRKLSIIASDIEHLLKEGSFEPSTFVFEAQKALSSLGTVTPKASYN 660
           LRL NGE+ CHL  +RKLS+IAS+IEHLLKE   EPSTFV E Q++LS LG +TPKAS  
Sbjct: 601 LRLANGEICCHLTIMRKLSMIASNIEHLLKEECIEPSTFVCEVQRSLSKLGAITPKASCY 660

Query: 661 LFDFRKLLQSFTLNHLEFSGKLKHIKAELVILDNDYEKPLHFVPGLPVGIPCQIILHNVL 720
             DFRKLL++FTLNHLE S KLKH+KAELVI DNDYEKPL+FVPGLPVGI CQIILHNV 
Sbjct: 661 SLDFRKLLKTFTLNHLEISKKLKHVKAELVIPDNDYEKPLYFVPGLPVGILCQIILHNVS 720

Query: 721 SERKLWVRISMDGMTSQFVFLDF-PVFGGCDEVREFTYVVPFYRTPKASSFIARICIGLE 780
           SERKLW RI+MD  TSQF+FLDF P+ GGCDEVREFTY VPFYRTPKASSFIARICIGLE
Sbjct: 721 SERKLWFRITMDNTTSQFIFLDFLPLGGGCDEVREFTYTVPFYRTPKASSFIARICIGLE 780

Query: 781 CCFESAEVCKRHRHGGPRRDLAYICKEKEVYLSMIN 814
           C FES EV    R GGP+RDLAYICKEKEVYLSMI+
Sbjct: 781 CWFESDEV--NERRGGPKRDLAYICKEKEVYLSMIH 812

BLAST of MS016760 vs. ExPASy TrEMBL
Match: A0A1S3CKJ8 (protein SIEL isoform X1 OS=Cucumis melo OX=3656 GN=LOC103501508 PE=4 SV=1)

HSP 1 Score: 1261.5 bits (3263), Expect = 0.0e+00
Identity = 644/817 (78.82%), Postives = 712/817 (87.15%), Query Frame = 0

Query: 1   MAERDRELVSAIDELDDGSFLSLCFGPSVSIRTWLLNNAERFQIRPSLLFTVFLGFTKDP 60
           MAE+D EL+S ++E+D+ SFLSLCFGPSVSIRTWLLNNAERFQ+RPSLLFTVFLGFTKDP
Sbjct: 1   MAEQDLELISTLNEIDEQSFLSLCFGPSVSIRTWLLNNAERFQLRPSLLFTVFLGFTKDP 60

Query: 61  YPYVRKAALDGLVGLGNNVFEDGGMIEGCYYRAIELLNDMEDCVRSAAVRVVITWGLMLA 120
           YPYVRKAALDGL  LGN VFEDGGMIEGCY RAIELLNDMED VRSAA+RVVITWGLMLA
Sbjct: 61  YPYVRKAALDGLSSLGNTVFEDGGMIEGCYCRAIELLNDMEDYVRSAAIRVVITWGLMLA 120

Query: 121 AHSPERKRYLSDEIFINLCSMTRDMSMKVRVNAFDAIKKLEIVSEDILLQSVSKRVLGIF 180
           AH+PERK+ L DEIF+NLCSMTRDM+MKVRVNAFDAI++LEIVSED+LLQSVSKRVL IF
Sbjct: 121 AHNPERKQQLFDEIFVNLCSMTRDMNMKVRVNAFDAIRRLEIVSEDLLLQSVSKRVLSIF 180

Query: 181 KGKRPLVQCSTEQLEMLALDVAGAFVHGVEDEFYQVRKSACDALYNLPILSTKFAGESLN 240
           KGK+ LVQCSTEQLE+LAL+VAGAFVHG+EDEFYQVR+SACDA++NL ILSTKFAGE+L+
Sbjct: 181 KGKKSLVQCSTEQLELLALNVAGAFVHGIEDEFYQVRRSACDAMFNLIILSTKFAGEALS 240

Query: 241 LLMDVLNDDSVSVRLQALETLHHMAMSKCLKLQEAHMHMFLGALSDSNGHVRSALRKLLK 300
           LLMD+LNDDSVSVRLQALETLHHMA S CLKLQEAHMHMFL AL D++GHVRSALRKLLK
Sbjct: 241 LLMDMLNDDSVSVRLQALETLHHMAKSNCLKLQEAHMHMFLNALKDNDGHVRSALRKLLK 300

Query: 301 LAKLPDLETFQLSFNGLLKSLESYPQDESDVLSVLFHMGQNHVNMAASIIQGVFKQIDPT 360
           L KLPDL TFQLSFNGLL+SLESYPQDESDVLSVLFHMGQNHVNM  SII+ VF+QIDPT
Sbjct: 301 LVKLPDLVTFQLSFNGLLESLESYPQDESDVLSVLFHMGQNHVNMVDSIIKDVFEQIDPT 360

Query: 361 SEGELGFDSVKVTAYIVLAISAPVLDDHTLRIPSRLFSYAVTLLGKISHALGDIMDQSTF 420
           SEG+L FDSVKV AYIVLAISA  LD+HTLRIP R+FSYA TLLG+ISHALGDIMDQST 
Sbjct: 361 SEGKLEFDSVKVLAYIVLAISALALDNHTLRIPPRVFSYAATLLGRISHALGDIMDQSTI 420

Query: 421 FAYLLENCKNIGSSDLVLNPEGSPCSPTPGGSINDI--LVSLRTTAMINEQQHKHDDTIE 480
           FAYLL N K+IG SDL  N E + CS T G S+NDI  + SL+  AMI+EQ  K DD IE
Sbjct: 421 FAYLLHNSKHIGLSDLGFNSEVASCSATCGSSVNDIPAIASLKIPAMIHEQGQKDDDAIE 480

Query: 481 SIKTVLSKVQDIWPLIQSGFLHQVLRTLRFCKEALGTFTHGSDTSSGALAFTLQYLKIIK 540
           SIKT+L KVQDIWPLIQSG LH+VLRTLRFCKEALG  T+G++  +GALAFT QYLKI+K
Sbjct: 481 SIKTILLKVQDIWPLIQSGVLHEVLRTLRFCKEALGVLTYGTNKYNGALAFTSQYLKILK 540

Query: 541 LVAKVWNLMSAKLNCLRKIGEWELLLGKLERGLKELRSRFIGFSKEEELHILELMLVTYT 600
           LVAKVWNLMS K +     GEW LLLGKLERGLKELRSRFIG +KEEE HILELMLVT  
Sbjct: 541 LVAKVWNLMSLKHSYPHGTGEWGLLLGKLERGLKELRSRFIGLTKEEEQHILELMLVTCI 600

Query: 601 LRLYNGEVWCHLMTLRKLSIIASDIEHLLKEGSFEPSTFVFEAQKALSSLGTVTPKASYN 660
           L L +GEV CHL +LRKLS IAS+IE+LLKE   EPSTFV E Q++LS+LGT+TPKA   
Sbjct: 601 LGLSSGEVCCHLTSLRKLSTIASNIENLLKEEFKEPSTFVCEVQRSLSNLGTITPKALCT 660

Query: 661 LFDFRKLLQSFTLNHLEFSGKLKHIKAELVILDNDYEKPLHFVPGLPVGIPCQIILHNVL 720
             D R++L+ FTL HLE S +LKHIKAELVI DN+YEKPL+FVPGLPVGIPCQIILHNV 
Sbjct: 661 SLDLRQMLKYFTLGHLEISEELKHIKAELVISDNNYEKPLYFVPGLPVGIPCQIILHNVP 720

Query: 721 SERKLWVRISMDGMTSQFVFLDFPVFGGCDEVREFTYVVPFYRTPKASSFIARICIGLEC 780
           SERKLW RI+MD MTSQF+FLDF   GGCDEVREF Y VPFYRTPKASSFIA+ICIGLEC
Sbjct: 721 SERKLWFRITMDNMTSQFIFLDFLSLGGCDEVREFMYTVPFYRTPKASSFIAKICIGLEC 780

Query: 781 CFESAEVCKRHRHGGPRRDLAYICKEKEVYLSMINKG 816
            FE+AEV    R GGP+ DLAYICKEKEVYLSMI KG
Sbjct: 781 WFENAEV-NDERRGGPKCDLAYICKEKEVYLSMIQKG 816

BLAST of MS016760 vs. TAIR 10
Match: AT3G08800.1 (ARM repeat superfamily protein )

HSP 1 Score: 590.9 bits (1522), Expect = 1.6e-168
Identity = 339/843 (40.21%), Postives = 493/843 (58.48%), Query Frame = 0

Query: 1   MAERDRELVSAIDELDDGSFLSLCFGPSVSIRTWLLNNAERFQIRPSLLFTVFLGFTKDP 60
           ++ER   + +A+ ++DD  F S+C G  +S R WLL NA+RF +  S+LFT+FLGF+KDP
Sbjct: 112 LSERTPSIAAALSKIDDEVFASICLGAPISSRLWLLRNADRFNVPSSVLFTLFLGFSKDP 171

Query: 61  YPYVRKAALDGLVGLGN-NVFEDGGMIEGCYYRAIELLNDMEDCVRSAAVRVVITWGLML 120
           YPY+RK ALDGL+ + N   F     +EGCY RA+ELL+D ED VRS+AVR V  WG ++
Sbjct: 172 YPYIRKVALDGLINICNAGDFNHTHAVEGCYTRAVELLSDAEDSVRSSAVRAVSVWGKVM 231

Query: 121 AAHSPE--RKRYLSDEIFINLCSMTRDMSMKVRVNAFDAIKKLEIVSEDILLQSVSKRVL 180
            A   E   +R  +D +F+ LCS+ RDMS+ VRV  F A   +   SE I+LQ++SK+VL
Sbjct: 232 IASKEEEMNRRDCTDAVFLQLCSVVRDMSVDVRVEVFKAFGIIGTASESIILQTLSKKVL 291

Query: 181 GIFKGKRPLVQCSTEQLEMLALDVAGAFVHGVEDEFYQVRKSACDALYNLPILSTKFAGE 240
           G  KGK+P    S    ++ +   AG ++HG EDEFY+VR++A D+ ++L + S KF  E
Sbjct: 292 GAGKGKKPQNLLSNGSADVSS--AAGVYIHGFEDEFYEVREAAVDSFHSLSVNSIKFPDE 351

Query: 241 SLNLLMDVLNDDSVSVRLQALETLHHMAMSKCLKLQEAHMHMFLGALSDSNGHVRSALRK 300
           ++ LLMD+L DD + VRL+AL+ LHH+A    LK+QE +M  FL A+ D++ ++R   R 
Sbjct: 352 AVYLLMDMLYDDYMVVRLKALKALHHIADLGNLKIQETYMPAFLDAIVDTSENIRVEARN 411

Query: 301 LLKLAKLPDLETFQLSFNGLLKSLESYPQDESDVLSVLFHMGQNHVNMAASIIQGVFKQI 360
           +LKLAKLPDL+      +G+LKSLE YPQDE D+LS LFH GQNH N   S+++   +++
Sbjct: 412 ILKLAKLPDLKLVNKCIDGVLKSLEMYPQDEPDILSALFHFGQNHTNFLVSMVKRFSEKL 471

Query: 361 DPTSEGELGFDSVKVTAYIVLAISAPVLDDHTL-RIPSRLFSYAVTLLGKISHALGDIMD 420
              S  +  F+S +++A + L ISAP+ +  ++  IP   FSY++ +LGK S  L D+MD
Sbjct: 472 GTASGSKAEFNSRQLSASLTLIISAPLSNKQSITSIPPLAFSYSLAMLGKFSSGLHDMMD 531

Query: 421 QSTFFAYLLENCKNIGSS--------------------DLVLNPEGSPCSPTPGGSINDI 480
           Q    AY L +C  + SS                    DL  NP   P    P  S    
Sbjct: 532 QDMLLAY-LTHCAILSSSSGTEFNKGDVFFHAYRDSNADLAGNPVLLPGKDIPAES---K 591

Query: 481 LVSLRTTAMINEQQHKHDDTIESIKTVLSKVQDIWPLIQSGFLHQVLRTLRFCKEALGTF 540
            ++ +    I  Q       ++ +  +L K++  W L QSG   + LR LR CK+ L T 
Sbjct: 592 YMACKAELEIGNQ------ALKFVNHILLKIKAAWLLSQSGCSKEALRALRACKQELATL 651

Query: 541 THGSDTSSGALAFTLQYLKIIKLVAKVWNLMSAKLNCLRKIG-----EWELLLGKLERGL 600
           T  S  S G L F  QY+ +I+L+ +VW       N  R I      E ELL+ ++E  L
Sbjct: 652 TADSSISKGTLDFICQYVHVIELLVQVW----PHFNYSRHISTCSSVEVELLMEEVEIKL 711

Query: 601 KELRSRFIGFSKEEELHILELMLVTYTLRLYNGEVWCHLMTLRKLSIIASDIEHLLKEGS 660
            E+R RF G S EE L +LEL++    LRLY  E+ C L  + KLS   S +E   ++  
Sbjct: 712 MEIRCRFTGLSTEESL-VLELVIFGCLLRLYKFEICCRLSCMEKLSSTISQLELHHEQQC 771

Query: 661 FEPSTFVFEAQKALSSLGTVTPKASYNLFDFRKLLQSFTLNHLEFSGKLKHIKAELVILD 720
            +PS F+ E +K+L   G+     S  L D  K+ + F+     FS  L+ + AE+ +  
Sbjct: 772 TKPSDFLTETKKSLEEFGSSDDINSCRLLDLIKIFKCFSPEQFTFSVNLQCVSAEVEVPG 831

Query: 721 NDYEKPLHFVPGLPVGIPCQIILHNVLSERKLWVRISMDGMTSQFVFLDFPVFGGCDEVR 780
           N    P+ FVPGLPV IPC+I L NV  +  LW+RIS +  T QFV+LD  ++ G    +
Sbjct: 832 NGPYSPISFVPGLPVAIPCEITLLNVPRDTCLWLRISRNDETCQFVYLDPNLYNGNGREK 891

Query: 781 EFTYVVPFYRTPKASSFIARICIGLECCFESAEVCKRHRHGGPRRDLAYICKEKEVYLSM 815
            F +    Y TP+A  F  R+ IG+EC FE  ++C R +  GP+  +AY+CKE+E++LS+
Sbjct: 892 RFMFTAVTYMTPRAVVFTLRVSIGIECLFE--DICYRKQRHGPKHPVAYLCKEREIHLSL 935