MS016749 (gene) Bitter gourd (TR) v1

BLAST of MS016749 vs. NCBI nr
Match: XP_022145676.1 (dnaJ protein ERDJ3A [Momordica charantia])

HSP 1 Score: 995.3 bits (2572), Expect = 1.9e-286
Identity = 511/527 (96.96%), Postives = 511/527 (96.96%), Query Frame = 0

Query: 1   SLQYHPDKNKQKGAQQKFEEINNAYEILSDEEKRKNFDMYGDEKGSPGFGYGHPGDQGGG 60
           SLQYHPDKNKQKGAQQKFEEINNAYEILSDEEKRKNFDMYGDEKGSPGFGYGHPGDQGGG
Sbjct: 52  SLQYHPDKNKQKGAQQKFEEINNAYEILSDEEKRKNFDMYGDEKGSPGFGYGHPGDQGGG 111

Query: 61  YTFFTNGGQRQGQWQSTGGGQGASQSFSFSFGGPSGSNPFGFGMEDIFSNFFGGGGNFGG 120
           YTFFTNGGQRQGQWQSTGGGQGASQSFSFSFGGPSGSN FGFGMEDIFSNFFGGGGNFGG
Sbjct: 112 YTFFTNGGQRQGQWQSTGGGQGASQSFSFSFGGPSGSNSFGFGMEDIFSNFFGGGGNFGG 171

Query: 121 FGGNSKAQTGSRTRSAPSTRAINKQVFRKEIVDQGMAWLLFPSTSSLKGVDHVESIIEEV 180
           FGGNSKAQTGSRTRSAPSTRAINKQVFRKEIVDQGMAWLLFPSTSSLKGVDHVESIIEEV
Sbjct: 172 FGGNSKAQTGSRTRSAPSTRAINKQVFRKEIVDQGMAWLLFPSTSSLKGVDHVESIIEEV 231

Query: 181 ASSLQGALKVGRIDCEAESSLCKDLGIYPHRTPRIFVYSYIKSTQGSLVEYSGDVAVKNL 240
           ASSLQGALKVGRIDCEAESSLCKDLGIYPHRTPRIFVYSYIKSTQGSLVEYSGDVAVKNL
Sbjct: 232 ASSLQGALKVGRIDCEAESSLCKDLGIYPHRTPRIFVYSYIKSTQGSLVEYSGDVAVKNL 291

Query: 241 KSFCQEHLPRFSQRVDLRQFDFSSSNGGGLPTLMLLSTKKDTPVIWRVLSGLYRRKFSFY 300
           KSFCQEHLPRFSQRVDLRQFDFS SNGGGLPTLMLLSTKKDTPVIWRVLSGLYRRKFSFY
Sbjct: 292 KSFCQEHLPRFSQRVDLRQFDFSPSNGGGLPTLMLLSTKKDTPVIWRVLSGLYRRKFSFY 351

Query: 301 DAEVNDVSDPTMKKLGVDALPAIVGWLSNGERHILRAGINVKDLRSAIDDLSNLFNGFGK 360
           DAEVNDVSDPTMKKLGVDALPAIVGWLSNGERHILRAGINVKDLRSAIDDLSNLFNGF K
Sbjct: 352 DAEVNDVSDPTMKKLGVDALPAIVGWLSNGERHILRAGINVKDLRSAIDDLSNLFNGFEK 411

Query: 361 KNKKTASRPTSKTPSDSAENQIPLLTGSDFDAICGEKTPVCVIGAFKSSKARNKLESILS 420
           KNKKTASRPTSKTPSDSAENQIPLLTGSDFDAICGEKTPVCVIG FKSSKARNKLESILS
Sbjct: 412 KNKKTASRPTSKTPSDSAENQIPLLTGSDFDAICGEKTPVCVIGXFKSSKARNKLESILS 471

Query: 421 MVRSLTFTEIEHVSQKTLSRRQNSSPGSKESISYALVDATKQPSFLKAFDNGELKKSDKV 480
           M           VSQKTLSRRQNSSPGSKESISYALVDATKQPSFLKAFDNGE KKSDKV
Sbjct: 472 M-----------VSQKTLSRRQNSSPGSKESISYALVDATKQPSFLKAFDNGEFKKSDKV 531

Query: 481 LVAYKRRKGKFAAHLGEMTEEEVEKFIGSVFNGDIPFTKTRQKPVFK 528
           LVAYKRRKGKFAAHLGEMTEEEVEKFIGSVFNGDIPFTKTRQKPVFK
Sbjct: 532 LVAYKRRKGKFAAHLGEMTEEEVEKFIGSVFNGDIPFTKTRQKPVFK 567

BLAST of MS016749 vs. NCBI nr
Match: XP_038905464.1 (dnaJ protein ERDJ3A isoform X1 [Benincasa hispida])

HSP 1 Score: 855.9 bits (2210), Expect = 1.8e-244
Identity = 443/538 (82.34%), Postives = 479/538 (89.03%), Query Frame = 0

Query: 1   SLQYHPDKNKQKGAQQKFEEINNAYEILSDEEKRKNFDMYGDEKGSPGFGYGHPGDQGGG 60
           SLQYHPDKNK+KGAQQKFEEINNAYEILSDEEKRKNFDMYG+EKGSPGFG G   DQGGG
Sbjct: 52  SLQYHPDKNKKKGAQQKFEEINNAYEILSDEEKRKNFDMYGNEKGSPGFGPGSSEDQGGG 111

Query: 61  YTFFTNGGQRQ-------GQWQSTGGGQGASQSFSFSFGGPSGSNPFGFGMEDIFSNFFG 120
           YTFFTNG  RQ       GQWQST GGQG SQSFSFSFGGPSGSNPFGFGMEDIFSNFFG
Sbjct: 112 YTFFTNGAGRQGQQPFGPGQWQST-GGQGGSQSFSFSFGGPSGSNPFGFGMEDIFSNFFG 171

Query: 121 GG----GNFGGFGGNSKAQTGSRTRSAPSTRAINKQVFRKEIVDQGMAWLLFPSTSSLKG 180
           GG    GNFG FG +SKAQ+GSRT S  S + INKQVF+KEIVDQGM WLLFP+TSSLKG
Sbjct: 172 GGFKDEGNFGSFGSHSKAQSGSRTSS--SIKVINKQVFKKEIVDQGMTWLLFPTTSSLKG 231

Query: 181 VDHVESIIEEVASSLQGALKVGRIDCEAESSLCKDLGIYPHRTPRIFVYSYIKSTQGSLV 240
           VDHV+SIIEEVASSLQGALKVGRIDC++ESSLCKDLGIYPHRTPRIFVYSYIKST+GSLV
Sbjct: 232 VDHVQSIIEEVASSLQGALKVGRIDCDSESSLCKDLGIYPHRTPRIFVYSYIKSTEGSLV 291

Query: 241 EYSGDVAVKNLKSFCQEHLPRFSQRVDLRQFDFSSSNGGGLPTLMLLSTKKDTPVIWRVL 300
           EYSGDVAVKNL+SFCQEH PRFSQRV+L+QFDFSSS+GG LPTLMLLSTKKDTPVIWRVL
Sbjct: 292 EYSGDVAVKNLRSFCQEHFPRFSQRVNLKQFDFSSSDGGRLPTLMLLSTKKDTPVIWRVL 351

Query: 301 SGLYRRKFSFYDAEVNDVSDPTMKKLGVDALPAIVGWLSNGERHILRAGINVKDLRSAID 360
           SGL+R++FSFYD EV DVSDP++KKLGVDALPAIVGWLSNGERH+LR GINVKDL+SAID
Sbjct: 352 SGLFRKQFSFYDTEVTDVSDPSVKKLGVDALPAIVGWLSNGERHVLRTGINVKDLKSAID 411

Query: 361 DLSNLFNGFGKKNKKTASRPTSKTPSDSAENQIPLLTGSDFDAICGEKTPVCVIGAFKSS 420
           DLSNL NGF KKN+K  SRP SKT SDSAENQIPLLTGS+FD++CGEKTPVCVIGAF+SS
Sbjct: 412 DLSNLLNGFEKKNRKAVSRPASKTQSDSAENQIPLLTGSNFDSVCGEKTPVCVIGAFRSS 471

Query: 421 KARNKLESILSMVRSLTFTEIEHVSQKTLSRRQNSSPGSKESISYALVDATKQPSFLKAF 480
           KARNKLE IL+M           VSQKTLSRRQNS+PGSKE+ISYALVDA+KQPSFL AF
Sbjct: 472 KARNKLELILNM-----------VSQKTLSRRQNSTPGSKETISYALVDASKQPSFLNAF 531

Query: 481 DNGELKKSDKVLVAYKRRKGKFAAHLGEMTEEEVEKFIGSVFNGDIPFTKTRQKPVFK 528
           D    K SDK+L+AYKRRKGKFAAHLGE+TEEEVEKFIGSV NGD+ FTKTRQKP+ K
Sbjct: 532 DGAGFKSSDKILIAYKRRKGKFAAHLGEITEEEVEKFIGSVLNGDVTFTKTRQKPILK 575

BLAST of MS016749 vs. NCBI nr
Match: TYK24944.1 (dnaJ protein ERDJ3A [Cucumis melo var. makuwa])

HSP 1 Score: 832.4 bits (2149), Expect = 2.2e-237
Identity = 430/535 (80.37%), Postives = 472/535 (88.22%), Query Frame = 0

Query: 1   SLQYHPDKNKQKGAQQKFEEINNAYEILSDEEKRKNFDMYGDEKGSPGFGYGHPGDQGGG 60
           SLQYHPDKNK+KGAQQKFEEINNAYEILSDEEKRKNFDMYGDEKG+PGFG G  GDQGGG
Sbjct: 52  SLQYHPDKNKKKGAQQKFEEINNAYEILSDEEKRKNFDMYGDEKGAPGFGAGPSGDQGGG 111

Query: 61  YTFFTNGGQRQ-------GQWQSTGGGQGASQSFSFSFGGPSGSNPFGFGMEDIFSNFFG 120
           YTFFTNGG RQ       GQWQST GGQG SQSFSFSFGGPSGSNPFGFGMEDIFSNFFG
Sbjct: 112 YTFFTNGGGRQGQQPFGPGQWQST-GGQGGSQSFSFSFGGPSGSNPFGFGMEDIFSNFFG 171

Query: 121 GG-GNFGGFGGNSKAQTGSRTRSAPSTRAINKQVFRKEIVDQGMAWLLFPSTSSLKGVDH 180
           GG  + G FGG+SKAQ+G   +S+ S + INKQ+ +KEIVDQGM WLLFP+TSSLKGVDH
Sbjct: 172 GGFKDKGSFGGHSKAQSG--PKSSSSIKVINKQLLKKEIVDQGMTWLLFPTTSSLKGVDH 231

Query: 181 VESIIEEVASSLQGALKVGRIDCEAESSLCKDLGIYPHRTPRIFVYSYIKSTQGSLVEYS 240
           V+SIIEEVAS+LQGALKVGRIDC++ESS CKDLGIYPHRTPRIFVYSYIKS +GSLVEYS
Sbjct: 232 VQSIIEEVASTLQGALKVGRIDCDSESSFCKDLGIYPHRTPRIFVYSYIKSNEGSLVEYS 291

Query: 241 GDVAVKNLKSFCQEHLPRFSQRVDLRQFDFSSSNGGGLPTLMLLSTKKDTPVIWRVLSGL 300
           GD+ VK+LK FCQEH PRFSQRV+L+QFDFSSSN G LPTLMLLSTKK+TPVIWRVLSGL
Sbjct: 292 GDIDVKSLKGFCQEHFPRFSQRVNLKQFDFSSSNRGRLPTLMLLSTKKETPVIWRVLSGL 351

Query: 301 YRRKFSFYDAEVNDVSDPTMKKLGVDALPAIVGWLSNGERHILRAGINVKDLRSAIDDLS 360
           +R+ F+FYDAEV D SDP++KKLGVDALPAIVGWLSNGERH+LR GINVKDL+SAIDDLS
Sbjct: 352 FRKLFNFYDAEVTDASDPSIKKLGVDALPAIVGWLSNGERHVLRTGINVKDLKSAIDDLS 411

Query: 361 NLFNGFGKKNKKTASRPTSKTPSDSAENQIPLLTGSDFDAICGEKTPVCVIGAFKSSKAR 420
           NL NGF KKN+K ASRPTSKT SDS ENQIPLLTGS+FD++CGEKTPVCVIGAF+SSKAR
Sbjct: 412 NLLNGFEKKNRKAASRPTSKTQSDSVENQIPLLTGSNFDSVCGEKTPVCVIGAFRSSKAR 471

Query: 421 NKLESILSMVRSLTFTEIEHVSQKTLSRRQNSSPGSKESISYALVDATKQPSFLKAFDNG 480
           NKLESIL M           VSQKTLSRRQNS+PGSKE+ISYALVDATKQ SFL AFD+ 
Sbjct: 472 NKLESILKM-----------VSQKTLSRRQNSTPGSKETISYALVDATKQVSFLNAFDSA 531

Query: 481 ELKKSDKVLVAYKRRKGKFAAHLGEMTEEEVEKFIGSVFNGDIPFTKTRQKPVFK 528
             K SDK+L+AYKRRKGKFAA+LGE+TEEEVEKFIGSV NGD+ FT TRQKP+ K
Sbjct: 532 GFKSSDKILIAYKRRKGKFAAYLGEITEEEVEKFIGSVLNGDVTFTNTRQKPILK 572

BLAST of MS016749 vs. NCBI nr
Match: XP_008442391.1 (PREDICTED: dnaJ protein ERDJ3A [Cucumis melo] >KAA0044188.1 dnaJ protein ERDJ3A [Cucumis melo var. makuwa])

HSP 1 Score: 827.8 bits (2137), Expect = 5.3e-236
Identity = 430/536 (80.22%), Postives = 472/536 (88.06%), Query Frame = 0

Query: 1   SLQYHPDKNKQKGAQQKFEEINNAYEILSDEEKRKNFDMYGDEKGSPGFGYGHPGDQGGG 60
           SLQYHPDKNK+KGAQQKFEEINNAYEILSDEEKRKNFDMYGDEKG+PGFG G  GDQGGG
Sbjct: 52  SLQYHPDKNKKKGAQQKFEEINNAYEILSDEEKRKNFDMYGDEKGAPGFGAGPSGDQGGG 111

Query: 61  YTFFTNGGQRQ-------GQWQSTGGGQGASQSFSFSFGGPSGSNPFGFGMEDIFSNFFG 120
           YTFFTNGG RQ       GQWQST GGQG SQSFSFSFGGPSGSNPFGFGMEDIFSNFFG
Sbjct: 112 YTFFTNGGGRQGQQPFGPGQWQST-GGQGGSQSFSFSFGGPSGSNPFGFGMEDIFSNFFG 171

Query: 121 GG-GNFGGFGGNSKAQTGSRTRSAPSTRAINKQVFRKEIVDQGMAWLLFPSTSSLKGVDH 180
           GG  + G FGG+SKAQ+G   +S+ S + INKQ+ +KEIVDQGM WLLFP+TSSLKGVDH
Sbjct: 172 GGFKDKGSFGGHSKAQSG--PKSSSSIKVINKQLLKKEIVDQGMTWLLFPTTSSLKGVDH 231

Query: 181 VESIIEEVASSLQGALKVGRIDCEAESSLCKDLGIYPHRTPRIFVYSYIKSTQGSLVEYS 240
           V+SIIEEVAS+LQGALKVGRIDC++ESS CKDLGIYPHRTPRIFVYSYIKS +GSLVEYS
Sbjct: 232 VQSIIEEVASTLQGALKVGRIDCDSESSFCKDLGIYPHRTPRIFVYSYIKSNEGSLVEYS 291

Query: 241 GDVAVKNLKSFCQEHLPRFSQRVDLRQFDFSSSNGGGLPTLMLLSTKKDTPVIWRVLSGL 300
           GD+ VK+LK FCQEH PRFSQRV+L+QFDFSSSN G LPTLMLLSTKK+TPVIWRVLSGL
Sbjct: 292 GDIDVKSLKGFCQEHFPRFSQRVNLKQFDFSSSNRGRLPTLMLLSTKKETPVIWRVLSGL 351

Query: 301 YRRKFSFYDAEVNDVSDPTMKKLGVDALPAIVGWLSNGERHILRAGINVKDLRSAIDDLS 360
           +R+ F+FYDAEV D SDP++KKLGVDALPAIVGWLSNGERH+LR GINVKDL+SAIDDLS
Sbjct: 352 FRKLFNFYDAEVTDASDPSIKKLGVDALPAIVGWLSNGERHVLRTGINVKDLKSAIDDLS 411

Query: 361 NLFNGFGKKNKKTASRPTSKTPSDSAENQIPLLTGSDFDAICGEKTPVCVIGAFKSSKAR 420
           NL NGF KKN+K ASRPTSKT SDS ENQIPLLTGS+FD++CGEKTPVCVIGAF+SSKAR
Sbjct: 412 NLLNGFEKKNRKAASRPTSKTQSDSVENQIPLLTGSNFDSVCGEKTPVCVIGAFRSSKAR 471

Query: 421 NKLESILSMVRSLTFTEIEHVSQKTLSRRQNSSPGSKESISYALVDATKQPSFLKAF-DN 480
           NKLESIL M           VSQKTLSRRQNS+PGSKE+ISYALVDATKQ SFL AF D+
Sbjct: 472 NKLESILKM-----------VSQKTLSRRQNSTPGSKETISYALVDATKQVSFLNAFVDS 531

Query: 481 GELKKSDKVLVAYKRRKGKFAAHLGEMTEEEVEKFIGSVFNGDIPFTKTRQKPVFK 528
              K SDK+L+AYKRRKGKFAA+LGE+TEEEVEKFIGSV NGD+ FT TRQKP+ K
Sbjct: 532 AGFKSSDKILIAYKRRKGKFAAYLGEITEEEVEKFIGSVLNGDVTFTNTRQKPILK 573

BLAST of MS016749 vs. NCBI nr
Match: XP_022935557.1 (dnaJ protein ERDJ3A [Cucurbita moschata] >KAG6580462.1 DnaJ protein ERDJ3A, partial [Cucurbita argyrosperma subsp. sororia] >KAG7017212.1 DnaJ protein ERDJ3A [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 820.1 bits (2117), Expect = 1.1e-233
Identity = 429/535 (80.19%), Postives = 470/535 (87.85%), Query Frame = 0

Query: 1   SLQYHPDKNKQKGAQQKFEEINNAYEILSDEEKRKNFDMYGDEKGSPGFGYGHPGDQGGG 60
           SLQYHPDKNK+K AQ KFEEINNAYEILSDEEKRKNFD+YGDEKGSPGFG G+ GDQGGG
Sbjct: 51  SLQYHPDKNKKKSAQHKFEEINNAYEILSDEEKRKNFDLYGDEKGSPGFGPGYSGDQGGG 110

Query: 61  YTFFTNGGQRQG------QWQSTGGGQGASQSFSFSFGGPS-GSNPFGFGMEDIFSNFFG 120
           +TFFTN G+RQG      QWQ T GGQG SQSFSFSFGGPS GSNPFGFGMEDIFSNFFG
Sbjct: 111 HTFFTNEGRRQGQQNFKPQWQGT-GGQGGSQSFSFSFGGPSGGSNPFGFGMEDIFSNFFG 170

Query: 121 GG-GNFGGFGGNSKAQTGSRTRSAPSTRAINKQVFRKEIVDQGMAWLLFPSTSSLKGVDH 180
           GG  + G FG + KAQ+GS++ S    + +NKQVF++E+V QG+ WLLFP TSSLKGVDH
Sbjct: 171 GGFKDEGNFGSHFKAQSGSKSSSI--IKVVNKQVFKQEVVGQGVTWLLFPITSSLKGVDH 230

Query: 181 VESIIEEVASSLQGALKVGRIDCEAESSLCKDLGIYPHRTPRIFVYSYIKSTQGSLVEYS 240
           V+SIIE+VASSLQGALK GRIDCE+ESSLCKDLGIYPHRTPRIFVYSYIKST GSLVEYS
Sbjct: 231 VQSIIEDVASSLQGALKAGRIDCESESSLCKDLGIYPHRTPRIFVYSYIKSTDGSLVEYS 290

Query: 241 GDVAVKNLKSFCQEHLPRFSQRVDLRQFDFSSSNGGGLPTLMLLSTKKDTPVIWRVLSGL 300
           GDVAVK+LKSFCQEHLPRFS+RVDLRQFDFSS+NGG LPTLMLLSTKKDTPVIWRVLSG+
Sbjct: 291 GDVAVKSLKSFCQEHLPRFSRRVDLRQFDFSSNNGGRLPTLMLLSTKKDTPVIWRVLSGM 350

Query: 301 YRRKFSFYDAEVNDVSDPTMKKLGVDALPAIVGWLSNGERHILRAGINVKDLRSAIDDLS 360
           Y ++FSFYDAEV DVSDPT+KKLGVDALPAIVGWLSNGERH+LR GINVKDLRSAIDDLS
Sbjct: 351 YNKQFSFYDAEVTDVSDPTIKKLGVDALPAIVGWLSNGERHVLRTGINVKDLRSAIDDLS 410

Query: 361 NLFNGFGKKNKKTASRPTSKTPSDSAENQIPLLTGSDFDAICGEKTPVCVIGAFKSSKAR 420
            L NGF KKN+K AS+P SKT SDSA+NQIPLLTG +FD+ICG+ TPVCVIGAF+SSKAR
Sbjct: 411 YLVNGFEKKNRKAASKPASKTQSDSAQNQIPLLTGFNFDSICGKNTPVCVIGAFRSSKAR 470

Query: 421 NKLESILSMVRSLTFTEIEHVSQKTLSRRQNSSPGSKESISYALVDATKQPSFLKAFDNG 480
           NKLE IL+M           VSQKTLSRRQ S+PGSKESISY+LVDATKQPSFLKAFD+ 
Sbjct: 471 NKLEMILNM-----------VSQKTLSRRQISAPGSKESISYSLVDATKQPSFLKAFDSA 530

Query: 481 ELKKSDKVLVAYKRRKGKFAAHLGEMTEEEVEKFIGSVFNGDIPFTKTRQKPVFK 528
             K SD++L+AYKRRK KFAAHLGEMTEEEVEKFIGSV NGDI FTKTRQKPV K
Sbjct: 531 GFKSSDQILIAYKRRKEKFAAHLGEMTEEEVEKFIGSVLNGDITFTKTRQKPVLK 571

BLAST of MS016749 vs. ExPASy Swiss-Prot
Match: Q9SR96 (DnaJ protein ERDJ3A OS=Arabidopsis thaliana OX=3702 GN=ERDJ3A PE=1 SV=1)

HSP 1 Score: 589.3 bits (1518), Expect = 4.2e-167
Identity = 311/536 (58.02%), Postives = 395/536 (73.69%), Query Frame = 0

Query: 1   SLQYHPDKNKQKGAQQKFEEINNAYEILSDEEKRKNFDMYGDEKGSPGFGYGHPGDQGGG 60
           SL+YHPDKNK KGAQ+KF EINNAYEILSDEEKRKN+D+YGDEKG PGF  G PG   GG
Sbjct: 51  SLKYHPDKNKDKGAQEKFAEINNAYEILSDEEKRKNYDLYGDEKGQPGFDSGFPGG-NGG 110

Query: 61  YTFFTNGG----QRQGQWQSTGGGQGASQSFSFSFGGPSGSNPFGFGMEDIFSNFFGGGG 120
           Y++ ++GG       G WQ+ GGG G S+SFSFSFGGPS S+ FGFGM+DIFS F GG  
Sbjct: 111 YSYSSSGGGFNFGGPGGWQNMGGG-GGSKSFSFSFGGPSESS-FGFGMDDIFSMFSGGSS 170

Query: 121 ----NFGGFGGNSKAQTGSRTRSAPSTRAINKQVFRKEIVDQGMAWLLFPSTSSLKGVDH 180
                FGGFG +S A++ S++ +  + + IN QV++K++VDQGM WLL     S +G  +
Sbjct: 171 KGKEQFGGFGSSSNAESKSKSSTVAAIKTINSQVYKKDVVDQGMTWLLLSYLPSQRGSQY 230

Query: 181 VESIIEEVASSLQGALKVGRIDCEAESSLCKDLGIYPHRTPRIFVYSYIKSTQGSLVEYS 240
            ESIIEEVA SLQGALKVGR++CE ESSLCK LGI P R PR+FVYSY  S + +L EY+
Sbjct: 231 HESIIEEVAESLQGALKVGRLNCETESSLCKQLGIVPRRAPRMFVYSYTSSGKATLAEYT 290

Query: 241 GDVAVKNLKSFCQEHLPRFSQRVDLRQFDFSSSNGGGLPTLMLLSTKKDTPVIWRVLSGL 300
            ++  K +KSFCQEHLPRFS+++DL  FD S+ +    P ++LLSTKKDTPVIWRVLSGL
Sbjct: 291 EELVAKKVKSFCQEHLPRFSKKIDLNTFDVSAVSSQKTPKVLLLSTKKDTPVIWRVLSGL 350

Query: 301 YRRKFSFYDAEVNDVSDPTMKKLGVDALPAIVGWLSNGERHILRAGINVKDLRSAIDDLS 360
           Y  +F FY+ EV+D SDP ++KLGVD  PAIVGWLSNGE+ +L+ GI VK+L+SA+ +L 
Sbjct: 351 YNGRFVFYNTEVHDTSDPKIQKLGVDKFPAIVGWLSNGEKQVLKTGITVKNLKSAVQELG 410

Query: 361 NLFNGFGKKNKKTASRPTSKTPSDSAENQIPLLTGSDFDAICGEKTPVCVIGAFKSSKAR 420
            L  G  KKNKK +S+  +    + +  +IPLL+  +FD+ICGE TPVC+IGAF+SS  +
Sbjct: 411 KLLEGLEKKNKKVSSKSQAGQAPNESSEKIPLLSRPNFDSICGENTPVCIIGAFRSSNGK 470

Query: 421 NKLESILSMVRSLTFTEIEHVSQKTLSRRQNSSPGSKESISYALVDATKQPSFLKAFDNG 480
            KL+SI+S            VSQK+LSRRQ S+ GS++++SY+L+DATKQ +FL + D  
Sbjct: 471 EKLQSIMS-----------KVSQKSLSRRQASTTGSQDTVSYSLLDATKQSAFLSSLDKS 530

Query: 481 ELK-KSDKVLVAYKRRKGKFAAHLGEMTEEEVEKFIGSVFNGDIPFTKTRQKPVFK 528
           E K  SDK+L+AYK R+GKFA   G+MT EEVEKF+ +V NGDI FTKTRQKP  K
Sbjct: 531 EFKTSSDKLLIAYKPRRGKFATFKGDMTIEEVEKFVAAVLNGDIQFTKTRQKPQIK 572

BLAST of MS016749 vs. ExPASy Swiss-Prot
Match: Q10MW6 (DnaJ protein ERDJ3A OS=Oryza sativa subsp. japonica OX=39947 GN=ERDJ3A PE=1 SV=2)

HSP 1 Score: 503.8 bits (1296), Expect = 2.3e-141
Identity = 274/544 (50.37%), Postives = 367/544 (67.46%), Query Frame = 0

Query: 1   SLQYHPDKNKQKGAQQKFEEINNAYEILSDEEKRKNFDMYGDEKGSPGFGYGHPGDQGGG 60
           SL+YHPDKNK KGAQ+KF EINNAY+ILSDEEKRKN+D+YGDEKG+PGFG G+ G++  G
Sbjct: 53  SLKYHPDKNKSKGAQEKFAEINNAYDILSDEEKRKNYDLYGDEKGNPGFGGGNFGNR-EG 112

Query: 61  YTFFTNGGQRQGQWQS-----TGGGQGASQSFSFSFG----GPSGSNPFGFGMEDIFSNF 120
           YT+FT GG +   + S     T GGQG +++FSFSFG    G  G NPF F   D+FSN 
Sbjct: 113 YTYFTGGGAKTSHFSSGDGWQTMGGQGNTKTFSFSFGGGNPGAGGGNPFNFDFGDVFSNI 172

Query: 121 FGGGGNFGG--FGGNSKAQTGSRT----RSAPSTRAINKQVFRKEIVDQGMAWLLFPSTS 180
           F GG   G    G   KA+ G+++     S+ + + +  Q+F KE  DQG+ WLL   T 
Sbjct: 173 FSGGSMGGSQHTGSAGKARRGTKSSGHDSSSVNIQEVTMQIFNKETADQGITWLLLFYTP 232

Query: 181 SLKGVDHVESIIEEVASSLQGALKVGRIDCEAESSLCKDLGIYPHRTPRIFVYSYIKSTQ 240
           + KG   +ES++E+VA SL GAL+ G+++C+ E +LCK  G+   ++ R+F+YSY  + +
Sbjct: 233 NTKGQFVLESVVEDVARSLDGALRAGKVNCDHEKALCKKAGVSIGKSARLFIYSYTTTEK 292

Query: 241 GSLVEYSGDVAVKNLKSFCQEHLPRFSQRVDLRQFDFSSSNGGGLPTLMLLSTKKDTPVI 300
           GSL EYSGD   K+LK+FCQEHLPRFS+RVD+ QF F S+    LP ++LLS KKDTP +
Sbjct: 293 GSLHEYSGDYDSKSLKTFCQEHLPRFSKRVDINQFSFPSNIIPNLPQVLLLSAKKDTPAM 352

Query: 301 WRVLSGLYRRKFSFYDAEVNDVSDPTMKKLGVDALPAIVGWLSNGERHILRAGINVKDLR 360
           WR +SG++R +  FYDAEV DVS P +K LGV  +PA++G   NGE  +L+ GI+VKDLR
Sbjct: 353 WRAVSGMFRSRLIFYDAEVQDVSHPLLKSLGVKNIPALIGRSVNGEEQLLKDGISVKDLR 412

Query: 361 SAIDDLSNLFNGFGKKNKKTASRPTSKT--PSDSAENQIPLLTGSDFDAICGEKTPVCVI 420
           S I +L NL   F KKNKK AS    K        EN+IPLLT S+F+ ICGEKT VC++
Sbjct: 413 SGIKELKNLLENFEKKNKKLASNQAKKPAHTDQPKENKIPLLTASNFEEICGEKTSVCIL 472

Query: 421 GAFKSSKARNKLESILSMVRSLTFTEIEHVSQKTLSRRQNSSPGSKESISYALVDATKQP 480
           G FKSSKA+  LE++LS            +SQKTL R QN + G+  +++YAL+D  KQ 
Sbjct: 473 GIFKSSKAKENLEAVLS-----------EISQKTLIRGQNYNSGN--AVAYALLDGNKQS 532

Query: 481 SFLKAFDNGELKKSDKVLVAYKRRKGKFAAHLGEMTEEEVEKFIGSVFNGDIPFTKTRQK 528
           +FL  FD    K SDK+L+AYK R+G++A +  E+T EE E+F+ SV NGD+  +   +K
Sbjct: 533 AFLSTFDKSAFKSSDKLLLAYKPRRGRYAVYDNEVTMEEAERFVVSVLNGDVQLSAAGRK 582

BLAST of MS016749 vs. ExPASy Swiss-Prot
Match: Q8EUM4 (Chaperone protein DnaJ OS=Mycoplasma penetrans (strain HF-2) OX=272633 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 78.6 bits (192), Expect = 2.4e-13
Identity = 51/115 (44.35%), Postives = 65/115 (56.52%), Query Frame = 0

Query: 1   SLQYHPDKNKQKGAQQKFEEINNAYEILSDEEKRKNFDMYGDEKGSPGFGYGHPGDQGGG 60
           ++QYHPD+NK+  A++KF+E+N AYE+LSD +KR N+D +G E     FG G   D    
Sbjct: 30  AMQYHPDRNKEPDAEEKFKEVNQAYEVLSDPDKRANYDRFGHEGVDGQFGGGAGFDP--- 89

Query: 61  YTFFTNGGQRQGQWQSTGGGQGASQSFSFSFGGPSGSNPFGFGMEDIFSNFFGGG 116
           +  F          Q  GGG+G    F  SFGG         G EDIFSNFFGGG
Sbjct: 90  FDIFN---------QFFGGGRGGGAHFEQSFGGS--------GFEDIFSNFFGGG 124

BLAST of MS016749 vs. ExPASy Swiss-Prot
Match: P35514 (Chaperone protein DnaJ OS=Lactococcus lactis subsp. lactis (strain IL1403) OX=272623 GN=dnaJ PE=3 SV=2)

HSP 1 Score: 72.8 bits (177), Expect = 1.3e-11
Identity = 51/116 (43.97%), Postives = 65/116 (56.03%), Query Frame = 0

Query: 1   SLQYHPDKNKQKGAQQKFEEINNAYEILSDEEKRKNFDMYGDEKGSPGFGYGHPGDQGGG 60
           S +YHPD NK++GA++K++E+  AYE LSDE+KR  +D YG+   + GFG          
Sbjct: 29  SKKYHPDLNKEEGAEEKYKEVQEAYETLSDEQKRAAYDQYGEAGANGGFG---------- 88

Query: 61  YTFFTNGGQRQGQWQSTGGGQGASQSFSFSFGGPSGSNPFGFGMEDIFSNFFGGGG 117
                            GGG G +  FS  FGG  GS  FG G EDIFS+FFGGGG
Sbjct: 89  -----------------GGGFGGASGFS-GFGG--GSGGFG-GFEDIFSSFFGGGG 113

BLAST of MS016749 vs. ExPASy Swiss-Prot
Match: A2RP20 (Chaperone protein DnaJ OS=Lactococcus lactis subsp. cremoris (strain MG1363) OX=416870 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 72.4 bits (176), Expect = 1.7e-11
Identity = 51/116 (43.97%), Postives = 64/116 (55.17%), Query Frame = 0

Query: 1   SLQYHPDKNKQKGAQQKFEEINNAYEILSDEEKRKNFDMYGDEKGSPGFGYGHPGDQGGG 60
           S +YHPD NK++GA+ K++E+  AYE LSDE+KR  +D YG+   + GFG          
Sbjct: 29  SKKYHPDLNKEEGAEDKYKEVQEAYETLSDEQKRAAYDQYGEAGANGGFG---------- 88

Query: 61  YTFFTNGGQRQGQWQSTGGGQGASQSFSFSFGGPSGSNPFGFGMEDIFSNFFGGGG 117
                            GGG G +  FS  FGG SG   FG G EDIFS+FFGGGG
Sbjct: 89  -----------------GGGFGGASGFS-GFGGASGG--FG-GFEDIFSSFFGGGG 113

BLAST of MS016749 vs. ExPASy TrEMBL
Match: A0A6J1CX10 (dnaJ protein ERDJ3A OS=Momordica charantia OX=3673 GN=LOC111015069 PE=4 SV=1)

HSP 1 Score: 995.3 bits (2572), Expect = 9.3e-287
Identity = 511/527 (96.96%), Postives = 511/527 (96.96%), Query Frame = 0

Query: 1   SLQYHPDKNKQKGAQQKFEEINNAYEILSDEEKRKNFDMYGDEKGSPGFGYGHPGDQGGG 60
           SLQYHPDKNKQKGAQQKFEEINNAYEILSDEEKRKNFDMYGDEKGSPGFGYGHPGDQGGG
Sbjct: 52  SLQYHPDKNKQKGAQQKFEEINNAYEILSDEEKRKNFDMYGDEKGSPGFGYGHPGDQGGG 111

Query: 61  YTFFTNGGQRQGQWQSTGGGQGASQSFSFSFGGPSGSNPFGFGMEDIFSNFFGGGGNFGG 120
           YTFFTNGGQRQGQWQSTGGGQGASQSFSFSFGGPSGSN FGFGMEDIFSNFFGGGGNFGG
Sbjct: 112 YTFFTNGGQRQGQWQSTGGGQGASQSFSFSFGGPSGSNSFGFGMEDIFSNFFGGGGNFGG 171

Query: 121 FGGNSKAQTGSRTRSAPSTRAINKQVFRKEIVDQGMAWLLFPSTSSLKGVDHVESIIEEV 180
           FGGNSKAQTGSRTRSAPSTRAINKQVFRKEIVDQGMAWLLFPSTSSLKGVDHVESIIEEV
Sbjct: 172 FGGNSKAQTGSRTRSAPSTRAINKQVFRKEIVDQGMAWLLFPSTSSLKGVDHVESIIEEV 231

Query: 181 ASSLQGALKVGRIDCEAESSLCKDLGIYPHRTPRIFVYSYIKSTQGSLVEYSGDVAVKNL 240
           ASSLQGALKVGRIDCEAESSLCKDLGIYPHRTPRIFVYSYIKSTQGSLVEYSGDVAVKNL
Sbjct: 232 ASSLQGALKVGRIDCEAESSLCKDLGIYPHRTPRIFVYSYIKSTQGSLVEYSGDVAVKNL 291

Query: 241 KSFCQEHLPRFSQRVDLRQFDFSSSNGGGLPTLMLLSTKKDTPVIWRVLSGLYRRKFSFY 300
           KSFCQEHLPRFSQRVDLRQFDFS SNGGGLPTLMLLSTKKDTPVIWRVLSGLYRRKFSFY
Sbjct: 292 KSFCQEHLPRFSQRVDLRQFDFSPSNGGGLPTLMLLSTKKDTPVIWRVLSGLYRRKFSFY 351

Query: 301 DAEVNDVSDPTMKKLGVDALPAIVGWLSNGERHILRAGINVKDLRSAIDDLSNLFNGFGK 360
           DAEVNDVSDPTMKKLGVDALPAIVGWLSNGERHILRAGINVKDLRSAIDDLSNLFNGF K
Sbjct: 352 DAEVNDVSDPTMKKLGVDALPAIVGWLSNGERHILRAGINVKDLRSAIDDLSNLFNGFEK 411

Query: 361 KNKKTASRPTSKTPSDSAENQIPLLTGSDFDAICGEKTPVCVIGAFKSSKARNKLESILS 420
           KNKKTASRPTSKTPSDSAENQIPLLTGSDFDAICGEKTPVCVIG FKSSKARNKLESILS
Sbjct: 412 KNKKTASRPTSKTPSDSAENQIPLLTGSDFDAICGEKTPVCVIGXFKSSKARNKLESILS 471

Query: 421 MVRSLTFTEIEHVSQKTLSRRQNSSPGSKESISYALVDATKQPSFLKAFDNGELKKSDKV 480
           M           VSQKTLSRRQNSSPGSKESISYALVDATKQPSFLKAFDNGE KKSDKV
Sbjct: 472 M-----------VSQKTLSRRQNSSPGSKESISYALVDATKQPSFLKAFDNGEFKKSDKV 531

Query: 481 LVAYKRRKGKFAAHLGEMTEEEVEKFIGSVFNGDIPFTKTRQKPVFK 528
           LVAYKRRKGKFAAHLGEMTEEEVEKFIGSVFNGDIPFTKTRQKPVFK
Sbjct: 532 LVAYKRRKGKFAAHLGEMTEEEVEKFIGSVFNGDIPFTKTRQKPVFK 567

BLAST of MS016749 vs. ExPASy TrEMBL
Match: A0A5D3DMX5 (DnaJ protein ERDJ3A OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G00600 PE=4 SV=1)

HSP 1 Score: 832.4 bits (2149), Expect = 1.0e-237
Identity = 430/535 (80.37%), Postives = 472/535 (88.22%), Query Frame = 0

Query: 1   SLQYHPDKNKQKGAQQKFEEINNAYEILSDEEKRKNFDMYGDEKGSPGFGYGHPGDQGGG 60
           SLQYHPDKNK+KGAQQKFEEINNAYEILSDEEKRKNFDMYGDEKG+PGFG G  GDQGGG
Sbjct: 52  SLQYHPDKNKKKGAQQKFEEINNAYEILSDEEKRKNFDMYGDEKGAPGFGAGPSGDQGGG 111

Query: 61  YTFFTNGGQRQ-------GQWQSTGGGQGASQSFSFSFGGPSGSNPFGFGMEDIFSNFFG 120
           YTFFTNGG RQ       GQWQST GGQG SQSFSFSFGGPSGSNPFGFGMEDIFSNFFG
Sbjct: 112 YTFFTNGGGRQGQQPFGPGQWQST-GGQGGSQSFSFSFGGPSGSNPFGFGMEDIFSNFFG 171

Query: 121 GG-GNFGGFGGNSKAQTGSRTRSAPSTRAINKQVFRKEIVDQGMAWLLFPSTSSLKGVDH 180
           GG  + G FGG+SKAQ+G   +S+ S + INKQ+ +KEIVDQGM WLLFP+TSSLKGVDH
Sbjct: 172 GGFKDKGSFGGHSKAQSG--PKSSSSIKVINKQLLKKEIVDQGMTWLLFPTTSSLKGVDH 231

Query: 181 VESIIEEVASSLQGALKVGRIDCEAESSLCKDLGIYPHRTPRIFVYSYIKSTQGSLVEYS 240
           V+SIIEEVAS+LQGALKVGRIDC++ESS CKDLGIYPHRTPRIFVYSYIKS +GSLVEYS
Sbjct: 232 VQSIIEEVASTLQGALKVGRIDCDSESSFCKDLGIYPHRTPRIFVYSYIKSNEGSLVEYS 291

Query: 241 GDVAVKNLKSFCQEHLPRFSQRVDLRQFDFSSSNGGGLPTLMLLSTKKDTPVIWRVLSGL 300
           GD+ VK+LK FCQEH PRFSQRV+L+QFDFSSSN G LPTLMLLSTKK+TPVIWRVLSGL
Sbjct: 292 GDIDVKSLKGFCQEHFPRFSQRVNLKQFDFSSSNRGRLPTLMLLSTKKETPVIWRVLSGL 351

Query: 301 YRRKFSFYDAEVNDVSDPTMKKLGVDALPAIVGWLSNGERHILRAGINVKDLRSAIDDLS 360
           +R+ F+FYDAEV D SDP++KKLGVDALPAIVGWLSNGERH+LR GINVKDL+SAIDDLS
Sbjct: 352 FRKLFNFYDAEVTDASDPSIKKLGVDALPAIVGWLSNGERHVLRTGINVKDLKSAIDDLS 411

Query: 361 NLFNGFGKKNKKTASRPTSKTPSDSAENQIPLLTGSDFDAICGEKTPVCVIGAFKSSKAR 420
           NL NGF KKN+K ASRPTSKT SDS ENQIPLLTGS+FD++CGEKTPVCVIGAF+SSKAR
Sbjct: 412 NLLNGFEKKNRKAASRPTSKTQSDSVENQIPLLTGSNFDSVCGEKTPVCVIGAFRSSKAR 471

Query: 421 NKLESILSMVRSLTFTEIEHVSQKTLSRRQNSSPGSKESISYALVDATKQPSFLKAFDNG 480
           NKLESIL M           VSQKTLSRRQNS+PGSKE+ISYALVDATKQ SFL AFD+ 
Sbjct: 472 NKLESILKM-----------VSQKTLSRRQNSTPGSKETISYALVDATKQVSFLNAFDSA 531

Query: 481 ELKKSDKVLVAYKRRKGKFAAHLGEMTEEEVEKFIGSVFNGDIPFTKTRQKPVFK 528
             K SDK+L+AYKRRKGKFAA+LGE+TEEEVEKFIGSV NGD+ FT TRQKP+ K
Sbjct: 532 GFKSSDKILIAYKRRKGKFAAYLGEITEEEVEKFIGSVLNGDVTFTNTRQKPILK 572

BLAST of MS016749 vs. ExPASy TrEMBL
Match: A0A5A7TMT4 (DnaJ protein ERDJ3A OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold236G005460 PE=4 SV=1)

HSP 1 Score: 827.8 bits (2137), Expect = 2.6e-236
Identity = 430/536 (80.22%), Postives = 472/536 (88.06%), Query Frame = 0

Query: 1   SLQYHPDKNKQKGAQQKFEEINNAYEILSDEEKRKNFDMYGDEKGSPGFGYGHPGDQGGG 60
           SLQYHPDKNK+KGAQQKFEEINNAYEILSDEEKRKNFDMYGDEKG+PGFG G  GDQGGG
Sbjct: 52  SLQYHPDKNKKKGAQQKFEEINNAYEILSDEEKRKNFDMYGDEKGAPGFGAGPSGDQGGG 111

Query: 61  YTFFTNGGQRQ-------GQWQSTGGGQGASQSFSFSFGGPSGSNPFGFGMEDIFSNFFG 120
           YTFFTNGG RQ       GQWQST GGQG SQSFSFSFGGPSGSNPFGFGMEDIFSNFFG
Sbjct: 112 YTFFTNGGGRQGQQPFGPGQWQST-GGQGGSQSFSFSFGGPSGSNPFGFGMEDIFSNFFG 171

Query: 121 GG-GNFGGFGGNSKAQTGSRTRSAPSTRAINKQVFRKEIVDQGMAWLLFPSTSSLKGVDH 180
           GG  + G FGG+SKAQ+G   +S+ S + INKQ+ +KEIVDQGM WLLFP+TSSLKGVDH
Sbjct: 172 GGFKDKGSFGGHSKAQSG--PKSSSSIKVINKQLLKKEIVDQGMTWLLFPTTSSLKGVDH 231

Query: 181 VESIIEEVASSLQGALKVGRIDCEAESSLCKDLGIYPHRTPRIFVYSYIKSTQGSLVEYS 240
           V+SIIEEVAS+LQGALKVGRIDC++ESS CKDLGIYPHRTPRIFVYSYIKS +GSLVEYS
Sbjct: 232 VQSIIEEVASTLQGALKVGRIDCDSESSFCKDLGIYPHRTPRIFVYSYIKSNEGSLVEYS 291

Query: 241 GDVAVKNLKSFCQEHLPRFSQRVDLRQFDFSSSNGGGLPTLMLLSTKKDTPVIWRVLSGL 300
           GD+ VK+LK FCQEH PRFSQRV+L+QFDFSSSN G LPTLMLLSTKK+TPVIWRVLSGL
Sbjct: 292 GDIDVKSLKGFCQEHFPRFSQRVNLKQFDFSSSNRGRLPTLMLLSTKKETPVIWRVLSGL 351

Query: 301 YRRKFSFYDAEVNDVSDPTMKKLGVDALPAIVGWLSNGERHILRAGINVKDLRSAIDDLS 360
           +R+ F+FYDAEV D SDP++KKLGVDALPAIVGWLSNGERH+LR GINVKDL+SAIDDLS
Sbjct: 352 FRKLFNFYDAEVTDASDPSIKKLGVDALPAIVGWLSNGERHVLRTGINVKDLKSAIDDLS 411

Query: 361 NLFNGFGKKNKKTASRPTSKTPSDSAENQIPLLTGSDFDAICGEKTPVCVIGAFKSSKAR 420
           NL NGF KKN+K ASRPTSKT SDS ENQIPLLTGS+FD++CGEKTPVCVIGAF+SSKAR
Sbjct: 412 NLLNGFEKKNRKAASRPTSKTQSDSVENQIPLLTGSNFDSVCGEKTPVCVIGAFRSSKAR 471

Query: 421 NKLESILSMVRSLTFTEIEHVSQKTLSRRQNSSPGSKESISYALVDATKQPSFLKAF-DN 480
           NKLESIL M           VSQKTLSRRQNS+PGSKE+ISYALVDATKQ SFL AF D+
Sbjct: 472 NKLESILKM-----------VSQKTLSRRQNSTPGSKETISYALVDATKQVSFLNAFVDS 531

Query: 481 GELKKSDKVLVAYKRRKGKFAAHLGEMTEEEVEKFIGSVFNGDIPFTKTRQKPVFK 528
              K SDK+L+AYKRRKGKFAA+LGE+TEEEVEKFIGSV NGD+ FT TRQKP+ K
Sbjct: 532 AGFKSSDKILIAYKRRKGKFAAYLGEITEEEVEKFIGSVLNGDVTFTNTRQKPILK 573

BLAST of MS016749 vs. ExPASy TrEMBL
Match: A0A1S3B542 (dnaJ protein ERDJ3A OS=Cucumis melo OX=3656 GN=LOC103486271 PE=4 SV=1)

HSP 1 Score: 827.8 bits (2137), Expect = 2.6e-236
Identity = 430/536 (80.22%), Postives = 472/536 (88.06%), Query Frame = 0

Query: 1   SLQYHPDKNKQKGAQQKFEEINNAYEILSDEEKRKNFDMYGDEKGSPGFGYGHPGDQGGG 60
           SLQYHPDKNK+KGAQQKFEEINNAYEILSDEEKRKNFDMYGDEKG+PGFG G  GDQGGG
Sbjct: 52  SLQYHPDKNKKKGAQQKFEEINNAYEILSDEEKRKNFDMYGDEKGAPGFGAGPSGDQGGG 111

Query: 61  YTFFTNGGQRQ-------GQWQSTGGGQGASQSFSFSFGGPSGSNPFGFGMEDIFSNFFG 120
           YTFFTNGG RQ       GQWQST GGQG SQSFSFSFGGPSGSNPFGFGMEDIFSNFFG
Sbjct: 112 YTFFTNGGGRQGQQPFGPGQWQST-GGQGGSQSFSFSFGGPSGSNPFGFGMEDIFSNFFG 171

Query: 121 GG-GNFGGFGGNSKAQTGSRTRSAPSTRAINKQVFRKEIVDQGMAWLLFPSTSSLKGVDH 180
           GG  + G FGG+SKAQ+G   +S+ S + INKQ+ +KEIVDQGM WLLFP+TSSLKGVDH
Sbjct: 172 GGFKDKGSFGGHSKAQSG--PKSSSSIKVINKQLLKKEIVDQGMTWLLFPTTSSLKGVDH 231

Query: 181 VESIIEEVASSLQGALKVGRIDCEAESSLCKDLGIYPHRTPRIFVYSYIKSTQGSLVEYS 240
           V+SIIEEVAS+LQGALKVGRIDC++ESS CKDLGIYPHRTPRIFVYSYIKS +GSLVEYS
Sbjct: 232 VQSIIEEVASTLQGALKVGRIDCDSESSFCKDLGIYPHRTPRIFVYSYIKSNEGSLVEYS 291

Query: 241 GDVAVKNLKSFCQEHLPRFSQRVDLRQFDFSSSNGGGLPTLMLLSTKKDTPVIWRVLSGL 300
           GD+ VK+LK FCQEH PRFSQRV+L+QFDFSSSN G LPTLMLLSTKK+TPVIWRVLSGL
Sbjct: 292 GDIDVKSLKGFCQEHFPRFSQRVNLKQFDFSSSNRGRLPTLMLLSTKKETPVIWRVLSGL 351

Query: 301 YRRKFSFYDAEVNDVSDPTMKKLGVDALPAIVGWLSNGERHILRAGINVKDLRSAIDDLS 360
           +R+ F+FYDAEV D SDP++KKLGVDALPAIVGWLSNGERH+LR GINVKDL+SAIDDLS
Sbjct: 352 FRKLFNFYDAEVTDASDPSIKKLGVDALPAIVGWLSNGERHVLRTGINVKDLKSAIDDLS 411

Query: 361 NLFNGFGKKNKKTASRPTSKTPSDSAENQIPLLTGSDFDAICGEKTPVCVIGAFKSSKAR 420
           NL NGF KKN+K ASRPTSKT SDS ENQIPLLTGS+FD++CGEKTPVCVIGAF+SSKAR
Sbjct: 412 NLLNGFEKKNRKAASRPTSKTQSDSVENQIPLLTGSNFDSVCGEKTPVCVIGAFRSSKAR 471

Query: 421 NKLESILSMVRSLTFTEIEHVSQKTLSRRQNSSPGSKESISYALVDATKQPSFLKAF-DN 480
           NKLESIL M           VSQKTLSRRQNS+PGSKE+ISYALVDATKQ SFL AF D+
Sbjct: 472 NKLESILKM-----------VSQKTLSRRQNSTPGSKETISYALVDATKQVSFLNAFVDS 531

Query: 481 GELKKSDKVLVAYKRRKGKFAAHLGEMTEEEVEKFIGSVFNGDIPFTKTRQKPVFK 528
              K SDK+L+AYKRRKGKFAA+LGE+TEEEVEKFIGSV NGD+ FT TRQKP+ K
Sbjct: 532 AGFKSSDKILIAYKRRKGKFAAYLGEITEEEVEKFIGSVLNGDVTFTNTRQKPILK 573

BLAST of MS016749 vs. ExPASy TrEMBL
Match: A0A6J1F5W9 (dnaJ protein ERDJ3A OS=Cucurbita moschata OX=3662 GN=LOC111442396 PE=4 SV=1)

HSP 1 Score: 820.1 bits (2117), Expect = 5.4e-234
Identity = 429/535 (80.19%), Postives = 470/535 (87.85%), Query Frame = 0

Query: 1   SLQYHPDKNKQKGAQQKFEEINNAYEILSDEEKRKNFDMYGDEKGSPGFGYGHPGDQGGG 60
           SLQYHPDKNK+K AQ KFEEINNAYEILSDEEKRKNFD+YGDEKGSPGFG G+ GDQGGG
Sbjct: 51  SLQYHPDKNKKKSAQHKFEEINNAYEILSDEEKRKNFDLYGDEKGSPGFGPGYSGDQGGG 110

Query: 61  YTFFTNGGQRQG------QWQSTGGGQGASQSFSFSFGGPS-GSNPFGFGMEDIFSNFFG 120
           +TFFTN G+RQG      QWQ T GGQG SQSFSFSFGGPS GSNPFGFGMEDIFSNFFG
Sbjct: 111 HTFFTNEGRRQGQQNFKPQWQGT-GGQGGSQSFSFSFGGPSGGSNPFGFGMEDIFSNFFG 170

Query: 121 GG-GNFGGFGGNSKAQTGSRTRSAPSTRAINKQVFRKEIVDQGMAWLLFPSTSSLKGVDH 180
           GG  + G FG + KAQ+GS++ S    + +NKQVF++E+V QG+ WLLFP TSSLKGVDH
Sbjct: 171 GGFKDEGNFGSHFKAQSGSKSSSI--IKVVNKQVFKQEVVGQGVTWLLFPITSSLKGVDH 230

Query: 181 VESIIEEVASSLQGALKVGRIDCEAESSLCKDLGIYPHRTPRIFVYSYIKSTQGSLVEYS 240
           V+SIIE+VASSLQGALK GRIDCE+ESSLCKDLGIYPHRTPRIFVYSYIKST GSLVEYS
Sbjct: 231 VQSIIEDVASSLQGALKAGRIDCESESSLCKDLGIYPHRTPRIFVYSYIKSTDGSLVEYS 290

Query: 241 GDVAVKNLKSFCQEHLPRFSQRVDLRQFDFSSSNGGGLPTLMLLSTKKDTPVIWRVLSGL 300
           GDVAVK+LKSFCQEHLPRFS+RVDLRQFDFSS+NGG LPTLMLLSTKKDTPVIWRVLSG+
Sbjct: 291 GDVAVKSLKSFCQEHLPRFSRRVDLRQFDFSSNNGGRLPTLMLLSTKKDTPVIWRVLSGM 350

Query: 301 YRRKFSFYDAEVNDVSDPTMKKLGVDALPAIVGWLSNGERHILRAGINVKDLRSAIDDLS 360
           Y ++FSFYDAEV DVSDPT+KKLGVDALPAIVGWLSNGERH+LR GINVKDLRSAIDDLS
Sbjct: 351 YNKQFSFYDAEVTDVSDPTIKKLGVDALPAIVGWLSNGERHVLRTGINVKDLRSAIDDLS 410

Query: 361 NLFNGFGKKNKKTASRPTSKTPSDSAENQIPLLTGSDFDAICGEKTPVCVIGAFKSSKAR 420
            L NGF KKN+K AS+P SKT SDSA+NQIPLLTG +FD+ICG+ TPVCVIGAF+SSKAR
Sbjct: 411 YLVNGFEKKNRKAASKPASKTQSDSAQNQIPLLTGFNFDSICGKNTPVCVIGAFRSSKAR 470

Query: 421 NKLESILSMVRSLTFTEIEHVSQKTLSRRQNSSPGSKESISYALVDATKQPSFLKAFDNG 480
           NKLE IL+M           VSQKTLSRRQ S+PGSKESISY+LVDATKQPSFLKAFD+ 
Sbjct: 471 NKLEMILNM-----------VSQKTLSRRQISAPGSKESISYSLVDATKQPSFLKAFDSA 530

Query: 481 ELKKSDKVLVAYKRRKGKFAAHLGEMTEEEVEKFIGSVFNGDIPFTKTRQKPVFK 528
             K SD++L+AYKRRK KFAAHLGEMTEEEVEKFIGSV NGDI FTKTRQKPV K
Sbjct: 531 GFKSSDQILIAYKRRKEKFAAHLGEMTEEEVEKFIGSVLNGDITFTKTRQKPVLK 571

BLAST of MS016749 vs. TAIR 10
Match: AT3G08970.1 (DNAJ heat shock N-terminal domain-containing protein )

HSP 1 Score: 589.3 bits (1518), Expect = 3.0e-168
Identity = 311/536 (58.02%), Postives = 395/536 (73.69%), Query Frame = 0

Query: 1   SLQYHPDKNKQKGAQQKFEEINNAYEILSDEEKRKNFDMYGDEKGSPGFGYGHPGDQGGG 60
           SL+YHPDKNK KGAQ+KF EINNAYEILSDEEKRKN+D+YGDEKG PGF  G PG   GG
Sbjct: 51  SLKYHPDKNKDKGAQEKFAEINNAYEILSDEEKRKNYDLYGDEKGQPGFDSGFPGG-NGG 110

Query: 61  YTFFTNGG----QRQGQWQSTGGGQGASQSFSFSFGGPSGSNPFGFGMEDIFSNFFGGGG 120
           Y++ ++GG       G WQ+ GGG G S+SFSFSFGGPS S+ FGFGM+DIFS F GG  
Sbjct: 111 YSYSSSGGGFNFGGPGGWQNMGGG-GGSKSFSFSFGGPSESS-FGFGMDDIFSMFSGGSS 170

Query: 121 ----NFGGFGGNSKAQTGSRTRSAPSTRAINKQVFRKEIVDQGMAWLLFPSTSSLKGVDH 180
                FGGFG +S A++ S++ +  + + IN QV++K++VDQGM WLL     S +G  +
Sbjct: 171 KGKEQFGGFGSSSNAESKSKSSTVAAIKTINSQVYKKDVVDQGMTWLLLSYLPSQRGSQY 230

Query: 181 VESIIEEVASSLQGALKVGRIDCEAESSLCKDLGIYPHRTPRIFVYSYIKSTQGSLVEYS 240
            ESIIEEVA SLQGALKVGR++CE ESSLCK LGI P R PR+FVYSY  S + +L EY+
Sbjct: 231 HESIIEEVAESLQGALKVGRLNCETESSLCKQLGIVPRRAPRMFVYSYTSSGKATLAEYT 290

Query: 241 GDVAVKNLKSFCQEHLPRFSQRVDLRQFDFSSSNGGGLPTLMLLSTKKDTPVIWRVLSGL 300
            ++  K +KSFCQEHLPRFS+++DL  FD S+ +    P ++LLSTKKDTPVIWRVLSGL
Sbjct: 291 EELVAKKVKSFCQEHLPRFSKKIDLNTFDVSAVSSQKTPKVLLLSTKKDTPVIWRVLSGL 350

Query: 301 YRRKFSFYDAEVNDVSDPTMKKLGVDALPAIVGWLSNGERHILRAGINVKDLRSAIDDLS 360
           Y  +F FY+ EV+D SDP ++KLGVD  PAIVGWLSNGE+ +L+ GI VK+L+SA+ +L 
Sbjct: 351 YNGRFVFYNTEVHDTSDPKIQKLGVDKFPAIVGWLSNGEKQVLKTGITVKNLKSAVQELG 410

Query: 361 NLFNGFGKKNKKTASRPTSKTPSDSAENQIPLLTGSDFDAICGEKTPVCVIGAFKSSKAR 420
            L  G  KKNKK +S+  +    + +  +IPLL+  +FD+ICGE TPVC+IGAF+SS  +
Sbjct: 411 KLLEGLEKKNKKVSSKSQAGQAPNESSEKIPLLSRPNFDSICGENTPVCIIGAFRSSNGK 470

Query: 421 NKLESILSMVRSLTFTEIEHVSQKTLSRRQNSSPGSKESISYALVDATKQPSFLKAFDNG 480
            KL+SI+S            VSQK+LSRRQ S+ GS++++SY+L+DATKQ +FL + D  
Sbjct: 471 EKLQSIMS-----------KVSQKSLSRRQASTTGSQDTVSYSLLDATKQSAFLSSLDKS 530

Query: 481 ELK-KSDKVLVAYKRRKGKFAAHLGEMTEEEVEKFIGSVFNGDIPFTKTRQKPVFK 528
           E K  SDK+L+AYK R+GKFA   G+MT EEVEKF+ +V NGDI FTKTRQKP  K
Sbjct: 531 EFKTSSDKLLIAYKPRRGKFATFKGDMTIEEVEKFVAAVLNGDIQFTKTRQKPQIK 572

BLAST of MS016749 vs. TAIR 10
Match: AT4G39960.1 (Molecular chaperone Hsp40/DnaJ family protein )

HSP 1 Score: 62.4 bits (150), Expect = 1.3e-09
Identity = 42/90 (46.67%), Postives = 52/90 (57.78%), Query Frame = 0

Query: 4   YHPDKNKQKGAQQKFEEINNAYEILSDEEKRKNFDMYGDEKGSPGFGYGHPGDQGGGYTF 63
           YHPD NK  GA+ KF+EI+NAYEILSD+EKR  +D YG E G  G G G  GD    +  
Sbjct: 112 YHPDVNKDAGAEDKFKEISNAYEILSDDEKRSLYDRYG-EAGVKGAGMGGMGDYSNPFDL 171

Query: 64  FTNGGQRQGQWQSTGGGQGASQSFSFSFGG 94
           F +  +  G     GGG G+  S S +  G
Sbjct: 172 FESLFEGMGGMGGMGGGMGSRGSRSRAIDG 200

BLAST of MS016749 vs. TAIR 10
Match: AT3G62600.1 (DNAJ heat shock family protein )

HSP 1 Score: 58.9 bits (141), Expect = 1.4e-08
Identity = 47/116 (40.52%), Postives = 60/116 (51.72%), Query Frame = 0

Query: 1   SLQYHPDKNK-QKGAQQKFEEINNAYEILSDEEKRKNFDMYGDEKGSPGFGYGHPGDQGG 60
           +L+YHPDKN+  + A +KF EINNAYE+LSDEEKR+ ++ YG+E               G
Sbjct: 50  ALKYHPDKNQGNEEATRKFAEINNAYEVLSDEEKREIYNKYGEE---------------G 109

Query: 61  GYTFFTNGGQRQGQWQSTGGGQGASQSFSFSFGGPSGSNPFGFGMEDIFSNFFGGG 116
              F  NGG+        GGG G                  G  M+DIFS+FFGGG
Sbjct: 110 LKQFSANGGR--------GGGGG------------------GMNMQDIFSSFFGGG 124

BLAST of MS016749 vs. TAIR 10
Match: AT2G22360.1 (DNAJ heat shock family protein )

HSP 1 Score: 56.6 bits (135), Expect = 6.9e-08
Identity = 36/81 (44.44%), Postives = 49/81 (60.49%), Query Frame = 0

Query: 4   YHPDKNKQKGAQQKFEEINNAYEILSDEEKRKNFDMYGDE--KGSPGFGYGHPGDQGGGY 63
           YHPD NK  GA++KF+EI+NAYE+LSD+EK+  +D YG+   KG+ GFG    GD    +
Sbjct: 113 YHPDVNKDPGAEEKFKEISNAYEVLSDDEKKSLYDRYGEAGLKGAAGFG---NGDFSNPF 172

Query: 64  TFFTNGGQRQGQWQSTGGGQG 83
             F         ++  GGG G
Sbjct: 173 DLF------DSLFEGFGGGMG 184

BLAST of MS016749 vs. TAIR 10
Match: AT1G80030.1 (Molecular chaperone Hsp40/DnaJ family protein )

HSP 1 Score: 53.1 bits (126), Expect = 7.7e-07
Identity = 48/133 (36.09%), Postives = 60/133 (45.11%), Query Frame = 0

Query: 3   QYHPDKNKQKGAQQKFEEINNAYEILSDEEKRKNFDMYGDEKGSPGFGYGHPGDQGGGYT 62
           QYHPD NK+ GA +KF+EI+ AYE+LSDE+KR  +D YG                     
Sbjct: 101 QYHPDVNKEPGATEKFKEISAAYEVLSDEQKRALYDQYG--------------------- 160

Query: 63  FFTNGGQRQGQWQSTGGGQGASQSFSFSFGGPSGSNPFGFGMEDIFSNFFGGGGNFGGFG 122
                   +   +ST GG            GP  SNPF     D+F  FF  G + GGF 
Sbjct: 161 --------EAGVKSTVGGA----------SGPYTSNPF-----DLFETFF--GASMGGFP 187

Query: 123 GNSKAQTGSRTRS 136
           G  +A  G   RS
Sbjct: 221 GMDQADFGRTRRS 187