MS016742 (gene) Bitter gourd (TR) v1

BLAST of MS016742 vs. NCBI nr
Match: XP_022145601.1 (acyl-coenzyme A oxidase 4, peroxisomal [Momordica charantia] >XP_022145602.1 acyl-coenzyme A oxidase 4, peroxisomal [Momordica charantia])

HSP 1 Score: 862.4 bits (2227), Expect = 1.6e-246
Identity = 432/435 (99.31%), Postives = 432/435 (99.31%), Query Frame = 0

Query: 1   NIADDNDKNSRSSYFGLPALDVSVAFPQATSASAFPPSVSDYYQFNDLLTPEEQALRKKV 60
           N  DDNDKNSRSSYFGLPALDVSVAFPQATSASAFPPSVSDYYQFN LLTPEEQALRKKV
Sbjct: 2   NRQDDNDKNSRSSYFGLPALDVSVAFPQATSASAFPPSVSDYYQFNGLLTPEEQALRKKV 61

Query: 61  RQCVEKEIAPIMTQYWEKAEFPFHVVPKLGNLCIAGGTIKGYGCPGLSITGSAVATAEVA 120
           RQCVEKEIAPIMTQYWEKAEFPFHVVPKLGNLCIAGGTIKGYGCPGLSITGSAVATAEVA
Sbjct: 62  RQCVEKEIAPIMTQYWEKAEFPFHVVPKLGNLCIAGGTIKGYGCPGLSITGSAVATAEVA 121

Query: 121 RVDASCSTFILVHSSLAMLTIALCGSEEQKHKYLPSLAKLDTIACWALTEPENGSDASGL 180
           RVDASCSTFILVHSSLAMLTIALCGSEEQKHKYLPSLAKLDTIACWALTEPENGSDASGL
Sbjct: 122 RVDASCSTFILVHSSLAMLTIALCGSEEQKHKYLPSLAKLDTIACWALTEPENGSDASGL 181

Query: 181 RTTATKVEGGWLIEGKKRWIGNSTFADLLIIFARNTITNEINGFIMKKNAPGLVVTKIEN 240
           RTTATKVEGGWLIEGKKRWIGNSTFADLLIIFARNTITNEINGFIMKKNAPGLVVTKIEN
Sbjct: 182 RTTATKVEGGWLIEGKKRWIGNSTFADLLIIFARNTITNEINGFIMKKNAPGLVVTKIEN 241

Query: 241 KIGLRIVQNGDIVMNKVFVPDEDRLPGVNSFKDTNKVLAVSRVMVAWQPIGIAMGVYDMC 300
           KIGLRIVQNGDIVMNKVFVPDEDRLPGVNSFKDTNKVLAVSRVMVAWQPIGIAMGVYDMC
Sbjct: 242 KIGLRIVQNGDIVMNKVFVPDEDRLPGVNSFKDTNKVLAVSRVMVAWQPIGIAMGVYDMC 301

Query: 301 HRYLKEREQFGAPLAAFQVNQQKLVLMLGNVQAMFLVGWRLCKLYEKGTMTPGQASLGKA 360
           HRYLKEREQFGAPLAAFQVNQQKLVLMLGNVQAMFLVGWRLCKLYEKGTMTPGQASLGKA
Sbjct: 302 HRYLKEREQFGAPLAAFQVNQQKLVLMLGNVQAMFLVGWRLCKLYEKGTMTPGQASLGKA 361

Query: 361 WITLRARETVALGRELLGGNGILSDFLVAKAFCDLEPIYTYEGTYDINSLVTGREITGLA 420
           WITLRARETVALGRELLGGNGILSDFLVAKAFCDLEPIYTYEGTYDINSLVTGREITGLA
Sbjct: 362 WITLRARETVALGRELLGGNGILSDFLVAKAFCDLEPIYTYEGTYDINSLVTGREITGLA 421

Query: 421 SFKPAAAALTKRSRL 436
           SFKPAAAALTKRSRL
Sbjct: 422 SFKPAAAALTKRSRL 436

BLAST of MS016742 vs. NCBI nr
Match: XP_022934517.1 (acyl-coenzyme A oxidase 4, peroxisomal [Cucurbita moschata] >KAG7017216.1 Acyl-coenzyme A oxidase 4, peroxisomal [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 827.8 bits (2137), Expect = 4.4e-236
Identity = 409/435 (94.02%), Postives = 426/435 (97.93%), Query Frame = 0

Query: 1   NIADDNDKNSRSSYFGLPALDVSVAFPQATSASAFPPSVSDYYQFNDLLTPEEQALRKKV 60
           N  DD DKNSRS+YFG PALDVSVAFPQAT+AS FPPSVSDYYQFNDLLTPEEQALRKKV
Sbjct: 8   NREDDKDKNSRSAYFGSPALDVSVAFPQATAASVFPPSVSDYYQFNDLLTPEEQALRKKV 67

Query: 61  RQCVEKEIAPIMTQYWEKAEFPFHVVPKLGNLCIAGGTIKGYGCPGLSITGSAVATAEVA 120
           RQC+EKEIAPIMT+YWEKAEFPFHV+PKLG+LCIAGGTIKGYGCPG++ITGSA+ATAEVA
Sbjct: 68  RQCMEKEIAPIMTKYWEKAEFPFHVIPKLGDLCIAGGTIKGYGCPGMTITGSAIATAEVA 127

Query: 121 RVDASCSTFILVHSSLAMLTIALCGSEEQKHKYLPSLAKLDTIACWALTEPENGSDASGL 180
           RVDASCSTFILVHSSLAMLTIALCGSEEQK KYLPSLAKLDTIACWALTEPENGSDASGL
Sbjct: 128 RVDASCSTFILVHSSLAMLTIALCGSEEQKQKYLPSLAKLDTIACWALTEPENGSDASGL 187

Query: 181 RTTATKVEGGWLIEGKKRWIGNSTFADLLIIFARNTITNEINGFIMKKNAPGLVVTKIEN 240
           RTTATKVEGGWLIEGKKRWIGNSTFADLL+IFARNTITNEINGFI+KKN+PGL+VTKIEN
Sbjct: 188 RTTATKVEGGWLIEGKKRWIGNSTFADLLVIFARNTITNEINGFIIKKNSPGLMVTKIEN 247

Query: 241 KIGLRIVQNGDIVMNKVFVPDEDRLPGVNSFKDTNKVLAVSRVMVAWQPIGIAMGVYDMC 300
           KIGLRIVQNGDIVMNKVFVPDEDRLPGVNSFKDTNKVLAVSRVMVAWQPIGIAMGVYDMC
Sbjct: 248 KIGLRIVQNGDIVMNKVFVPDEDRLPGVNSFKDTNKVLAVSRVMVAWQPIGIAMGVYDMC 307

Query: 301 HRYLKEREQFGAPLAAFQVNQQKLVLMLGNVQAMFLVGWRLCKLYEKGTMTPGQASLGKA 360
           HRYLKEREQFGAPLAAFQVNQQKLVLMLGNVQAMFL+GWRLCKLYEKGTMTPGQASLGKA
Sbjct: 308 HRYLKEREQFGAPLAAFQVNQQKLVLMLGNVQAMFLMGWRLCKLYEKGTMTPGQASLGKA 367

Query: 361 WITLRARETVALGRELLGGNGILSDFLVAKAFCDLEPIYTYEGTYDINSLVTGREITGLA 420
           WITLRARETVALGRELLGGNGILSDFLVAKAFCDLEPIYTYEGTYDIN+LVTGREITG+A
Sbjct: 368 WITLRARETVALGRELLGGNGILSDFLVAKAFCDLEPIYTYEGTYDINTLVTGREITGVA 427

Query: 421 SFKPAAAALTKRSRL 436
           SFKP  A+L KRSRL
Sbjct: 428 SFKP--ASLAKRSRL 440

BLAST of MS016742 vs. NCBI nr
Match: KAG6580466.1 (Acyl-coenzyme A oxidase 4, peroxisomal, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 827.8 bits (2137), Expect = 4.4e-236
Identity = 409/435 (94.02%), Postives = 426/435 (97.93%), Query Frame = 0

Query: 1   NIADDNDKNSRSSYFGLPALDVSVAFPQATSASAFPPSVSDYYQFNDLLTPEEQALRKKV 60
           N  DD DKNSRS+YFG PALDVSVAFPQAT+AS FPPSVSDYYQFNDLLTPEEQALRKKV
Sbjct: 75  NREDDKDKNSRSAYFGSPALDVSVAFPQATAASVFPPSVSDYYQFNDLLTPEEQALRKKV 134

Query: 61  RQCVEKEIAPIMTQYWEKAEFPFHVVPKLGNLCIAGGTIKGYGCPGLSITGSAVATAEVA 120
           RQC+EKEIAPIMT+YWEKAEFPFHV+PKLG+LCIAGGTIKGYGCPG++ITGSA+ATAEVA
Sbjct: 135 RQCMEKEIAPIMTKYWEKAEFPFHVIPKLGDLCIAGGTIKGYGCPGMTITGSAIATAEVA 194

Query: 121 RVDASCSTFILVHSSLAMLTIALCGSEEQKHKYLPSLAKLDTIACWALTEPENGSDASGL 180
           RVDASCSTFILVHSSLAMLTIALCGSEEQK KYLPSLAKLDTIACWALTEPENGSDASGL
Sbjct: 195 RVDASCSTFILVHSSLAMLTIALCGSEEQKQKYLPSLAKLDTIACWALTEPENGSDASGL 254

Query: 181 RTTATKVEGGWLIEGKKRWIGNSTFADLLIIFARNTITNEINGFIMKKNAPGLVVTKIEN 240
           RTTATKVEGGWLIEGKKRWIGNSTFADLL+IFARNTITNEINGFI+KKN+PGL+VTKIEN
Sbjct: 255 RTTATKVEGGWLIEGKKRWIGNSTFADLLVIFARNTITNEINGFIIKKNSPGLMVTKIEN 314

Query: 241 KIGLRIVQNGDIVMNKVFVPDEDRLPGVNSFKDTNKVLAVSRVMVAWQPIGIAMGVYDMC 300
           KIGLRIVQNGDIVMNKVFVPDEDRLPGVNSFKDTNKVLAVSRVMVAWQPIGIAMGVYDMC
Sbjct: 315 KIGLRIVQNGDIVMNKVFVPDEDRLPGVNSFKDTNKVLAVSRVMVAWQPIGIAMGVYDMC 374

Query: 301 HRYLKEREQFGAPLAAFQVNQQKLVLMLGNVQAMFLVGWRLCKLYEKGTMTPGQASLGKA 360
           HRYLKEREQFGAPLAAFQVNQQKLVLMLGNVQAMFL+GWRLCKLYEKGTMTPGQASLGKA
Sbjct: 375 HRYLKEREQFGAPLAAFQVNQQKLVLMLGNVQAMFLMGWRLCKLYEKGTMTPGQASLGKA 434

Query: 361 WITLRARETVALGRELLGGNGILSDFLVAKAFCDLEPIYTYEGTYDINSLVTGREITGLA 420
           WITLRARETVALGRELLGGNGILSDFLVAKAFCDLEPIYTYEGTYDIN+LVTGREITG+A
Sbjct: 435 WITLRARETVALGRELLGGNGILSDFLVAKAFCDLEPIYTYEGTYDINTLVTGREITGVA 494

Query: 421 SFKPAAAALTKRSRL 436
           SFKP  A+L KRSRL
Sbjct: 495 SFKP--ASLAKRSRL 507

BLAST of MS016742 vs. NCBI nr
Match: XP_008442401.1 (PREDICTED: acyl-coenzyme A oxidase 4, peroxisomal [Cucumis melo] >KAA0044182.1 acyl-coenzyme A oxidase 4, peroxisomal [Cucumis melo var. makuwa] >TYK24950.1 acyl-coenzyme A oxidase 4, peroxisomal [Cucumis melo var. makuwa])

HSP 1 Score: 826.6 bits (2134), Expect = 9.8e-236
Identity = 408/435 (93.79%), Postives = 425/435 (97.70%), Query Frame = 0

Query: 1   NIADDNDKNSRSSYFGLPALDVSVAFPQATSASAFPPSVSDYYQFNDLLTPEEQALRKKV 60
           N  DD DKN+RS+YFGLPALDVSVAFPQATSAS+FPPSVSD+YQFNDLLTPEEQALRKKV
Sbjct: 8   NRQDDKDKNARSAYFGLPALDVSVAFPQATSASSFPPSVSDFYQFNDLLTPEEQALRKKV 67

Query: 61  RQCVEKEIAPIMTQYWEKAEFPFHVVPKLGNLCIAGGTIKGYGCPGLSITGSAVATAEVA 120
           RQCVEKE+APIMT+YWEKAEFPF VVPKLG+LCIAGGTIKGYGCPGLSITGSAVA AEVA
Sbjct: 68  RQCVEKEVAPIMTKYWEKAEFPFEVVPKLGSLCIAGGTIKGYGCPGLSITGSAVAVAEVA 127

Query: 121 RVDASCSTFILVHSSLAMLTIALCGSEEQKHKYLPSLAKLDTIACWALTEPENGSDASGL 180
           RVDASCSTFILVHSSLAMLTIALCGSEEQKHKYLPSLAK DT+ACWALTEPENGSDASGL
Sbjct: 128 RVDASCSTFILVHSSLAMLTIALCGSEEQKHKYLPSLAKFDTVACWALTEPENGSDASGL 187

Query: 181 RTTATKVEGGWLIEGKKRWIGNSTFADLLIIFARNTITNEINGFIMKKNAPGLVVTKIEN 240
           RTTATKVEGGW+IEGKKRWIGNSTFAD+L+IFARNTITNEINGFI+KKNAPGL VTKIEN
Sbjct: 188 RTTATKVEGGWVIEGKKRWIGNSTFADVLVIFARNTITNEINGFIIKKNAPGLTVTKIEN 247

Query: 241 KIGLRIVQNGDIVMNKVFVPDEDRLPGVNSFKDTNKVLAVSRVMVAWQPIGIAMGVYDMC 300
           KIGLRIVQNGDIVMNKVFVPDEDRLPGVNSFKDTNKVLAVSRVMVAWQPIGIAMGVYDMC
Sbjct: 248 KIGLRIVQNGDIVMNKVFVPDEDRLPGVNSFKDTNKVLAVSRVMVAWQPIGIAMGVYDMC 307

Query: 301 HRYLKEREQFGAPLAAFQVNQQKLVLMLGNVQAMFLVGWRLCKLYEKGTMTPGQASLGKA 360
           HRYLKEREQFGAPLAAFQ+NQQKLVLMLGNVQAMFL+GWRLCKLYEKGTMTPGQASLGKA
Sbjct: 308 HRYLKEREQFGAPLAAFQLNQQKLVLMLGNVQAMFLIGWRLCKLYEKGTMTPGQASLGKA 367

Query: 361 WITLRARETVALGRELLGGNGILSDFLVAKAFCDLEPIYTYEGTYDINSLVTGREITGLA 420
           WITLRARETV+LGRELLGGNGILSDFLVAKAFCDLEPIYTYEGTYDIN+LVTGREITG+A
Sbjct: 368 WITLRARETVSLGRELLGGNGILSDFLVAKAFCDLEPIYTYEGTYDINTLVTGREITGVA 427

Query: 421 SFKPAAAALTKRSRL 436
           SFKP  AAL KRSRL
Sbjct: 428 SFKP--AALAKRSRL 440

BLAST of MS016742 vs. NCBI nr
Match: XP_022983342.1 (acyl-coenzyme A oxidase 4, peroxisomal [Cucurbita maxima])

HSP 1 Score: 826.6 bits (2134), Expect = 9.8e-236
Identity = 408/435 (93.79%), Postives = 424/435 (97.47%), Query Frame = 0

Query: 1   NIADDNDKNSRSSYFGLPALDVSVAFPQATSASAFPPSVSDYYQFNDLLTPEEQALRKKV 60
           N  DD DKNSRS+YFG PALDVSVAFPQAT+AS FPPSVSDYYQFNDLL PEEQALRKKV
Sbjct: 8   NREDDKDKNSRSAYFGSPALDVSVAFPQATAASVFPPSVSDYYQFNDLLIPEEQALRKKV 67

Query: 61  RQCVEKEIAPIMTQYWEKAEFPFHVVPKLGNLCIAGGTIKGYGCPGLSITGSAVATAEVA 120
           RQC+EKEIAPIMT+YWEKAEFPFH++PKLGNLCIAGGTIKGYGCPG++ITG A+ATAEVA
Sbjct: 68  RQCMEKEIAPIMTKYWEKAEFPFHIIPKLGNLCIAGGTIKGYGCPGMTITGCAIATAEVA 127

Query: 121 RVDASCSTFILVHSSLAMLTIALCGSEEQKHKYLPSLAKLDTIACWALTEPENGSDASGL 180
           RVDASCSTFILVHSSLAMLT+ALCGSEEQK KYLPSLAKLDTIACWALTEPENGSDASGL
Sbjct: 128 RVDASCSTFILVHSSLAMLTMALCGSEEQKQKYLPSLAKLDTIACWALTEPENGSDASGL 187

Query: 181 RTTATKVEGGWLIEGKKRWIGNSTFADLLIIFARNTITNEINGFIMKKNAPGLVVTKIEN 240
           RTTATKVEGGWLIEGKKRWIGNSTFADLL+IFARNTITNEINGFIMKKN+PGL+VTKIEN
Sbjct: 188 RTTATKVEGGWLIEGKKRWIGNSTFADLLVIFARNTITNEINGFIMKKNSPGLMVTKIEN 247

Query: 241 KIGLRIVQNGDIVMNKVFVPDEDRLPGVNSFKDTNKVLAVSRVMVAWQPIGIAMGVYDMC 300
           KIGLRIVQNGDIVMNKVFVPDEDRLPGVNSFKDTNKVLAVSRVMVAWQPIGIAMGVYDMC
Sbjct: 248 KIGLRIVQNGDIVMNKVFVPDEDRLPGVNSFKDTNKVLAVSRVMVAWQPIGIAMGVYDMC 307

Query: 301 HRYLKEREQFGAPLAAFQVNQQKLVLMLGNVQAMFLVGWRLCKLYEKGTMTPGQASLGKA 360
           HRYLKEREQFGAPLAAFQVNQQKLVLMLGNVQAMFLVGWRLCKLYEKGTMTPGQASLGKA
Sbjct: 308 HRYLKEREQFGAPLAAFQVNQQKLVLMLGNVQAMFLVGWRLCKLYEKGTMTPGQASLGKA 367

Query: 361 WITLRARETVALGRELLGGNGILSDFLVAKAFCDLEPIYTYEGTYDINSLVTGREITGLA 420
           WITLRARETVALGRELLGGNGILSDFLVAKAFCDLEPIYTYEGTYDIN+LVTGREITG+A
Sbjct: 368 WITLRARETVALGRELLGGNGILSDFLVAKAFCDLEPIYTYEGTYDINTLVTGREITGVA 427

Query: 421 SFKPAAAALTKRSRL 436
           SFKP  A+L KRSRL
Sbjct: 428 SFKP--ASLAKRSRL 440

BLAST of MS016742 vs. ExPASy Swiss-Prot
Match: Q96329 (Acyl-coenzyme A oxidase 4, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=ACX4 PE=1 SV=1)

HSP 1 Score: 731.1 bits (1886), Expect = 7.4e-210
Identity = 352/430 (81.86%), Postives = 394/430 (91.63%), Query Frame = 0

Query: 6   NDKNSRSSYFGLPALDVSVAFPQATSASAFPPSVSDYYQFNDLLTPEEQALRKKVRQCVE 65
           N+K  +SSYF LP +++SVAFPQAT AS FPP  SDYY FNDLLTPEEQA+RKKVR+C+E
Sbjct: 12  NEKKVKSSYFDLPPMEMSVAFPQATPASTFPPCTSDYYHFNDLLTPEEQAIRKKVRECME 71

Query: 66  KEIAPIMTQYWEKAEFPFHVVPKLGNLCIAGGTIKGYGCPGLSITGSAVATAEVARVDAS 125
           KE+APIMT+YWEKAEFPFH+ PKLG + +AGG+IKGYGCPGLSIT +A+ATAE+ARVDAS
Sbjct: 72  KEVAPIMTEYWEKAEFPFHITPKLGAMGVAGGSIKGYGCPGLSITANAIATAEIARVDAS 131

Query: 126 CSTFILVHSSLAMLTIALCGSEEQKHKYLPSLAKLDTIACWALTEPENGSDASGLRTTAT 185
           CSTFILVHSSL MLTIALCGSE QK KYLPSLA+L+T+ACWALTEP+NGSDASGL TTAT
Sbjct: 132 CSTFILVHSSLGMLTIALCGSEAQKEKYLPSLAQLNTVACWALTEPDNGSDASGLGTTAT 191

Query: 186 KVEGGWLIEGKKRWIGNSTFADLLIIFARNTITNEINGFIMKKNAPGLVVTKIENKIGLR 245
           KVEGGW I G+KRWIGNSTFADLLIIFARNT TN+INGFI+KK+APGL  TKI NKIGLR
Sbjct: 192 KVEGGWKINGQKRWIGNSTFADLLIIFARNTTTNQINGFIVKKDAPGLKATKIPNKIGLR 251

Query: 246 IVQNGDIVMNKVFVPDEDRLPGVNSFKDTNKVLAVSRVMVAWQPIGIAMGVYDMCHRYLK 305
           +VQNGDI++  VFVPDEDRLPGVNSF+DT+KVLAVSRVMVAWQPIGI+MG+YDMCHRYLK
Sbjct: 252 MVQNGDILLQNVFVPDEDRLPGVNSFQDTSKVLAVSRVMVAWQPIGISMGIYDMCHRYLK 311

Query: 306 EREQFGAPLAAFQVNQQKLVLMLGNVQAMFLVGWRLCKLYEKGTMTPGQASLGKAWITLR 365
           ER+QFGAPLAAFQ+NQQKLV MLGNVQAMFL+GWRLCKLYE G MTPGQASLGKAWI+ +
Sbjct: 312 ERKQFGAPLAAFQLNQQKLVQMLGNVQAMFLMGWRLCKLYETGQMTPGQASLGKAWISSK 371

Query: 366 ARETVALGRELLGGNGILSDFLVAKAFCDLEPIYTYEGTYDINSLVTGREITGLASFKPA 425
           ARET +LGRELLGGNGIL+DFLVAKAFCDLEPIYTYEGTYDIN+LVTGRE+TG+ASFKPA
Sbjct: 372 ARETASLGRELLGGNGILADFLVAKAFCDLEPIYTYEGTYDINTLVTGREVTGIASFKPA 431

Query: 426 AAALTKRSRL 436
                 RSRL
Sbjct: 432 T-----RSRL 436

BLAST of MS016742 vs. ExPASy Swiss-Prot
Match: Q20772 (Probable glutaryl-CoA dehydrogenase, mitochondrial OS=Caenorhabditis elegans OX=6239 GN=F54D5.7 PE=3 SV=1)

HSP 1 Score: 245.0 bits (624), Expect = 1.6e-63
Identity = 133/385 (34.55%), Postives = 218/385 (56.62%), Query Frame = 0

Query: 41  DYYQFNDLLTPEEQALRKKVRQCVEKEIAPIMTQYWEKAEFPFHVVPKLGNLCIAGGTIK 100
           D +Q +D LT +E++L    R+  ++ + P +T+ +   +F   ++P++G++ + G   +
Sbjct: 24  DAFQLSDQLTEDERSLMLSAREYCQERLLPRVTEAYRTEKFDPSLIPEMGSMGLLGAPYQ 83

Query: 101 GYGCPGLSITGSAVATAEVARVDASCSTFILVHSSLAMLTIALCGSEEQKHKYLPSLAKL 160
           GYGC G S  G  +   EV RVD+   + + V +SL +  I   GSE+QK KY+P LA  
Sbjct: 84  GYGCAGTSTVGYGLIAREVERVDSGYRSTMSVQTSLVIGPIYNYGSEDQKQKYIPDLASG 143

Query: 161 DTIACWALTEPENGSDASGLRTTAT--KVEGGWLIEGKKRWIGNSTFADLLIIFARNT-I 220
             I C+ LTEP +GS+  G+ T AT  +    + + G K WI NS  +D+++++AR+   
Sbjct: 144 KKIGCFGLTEPNHGSNPGGMETKATWDETTKTYKLNGSKTWISNSPVSDVMVVWARSARH 203

Query: 221 TNEINGFIMKKNAPGLVVTKIENKIGLRIVQNGDIVMNKVFVPDEDRLPGVNSFKDTNKV 280
            N+I GFI+++   GL   KIE K+ LR    G I M+ V VP+E+ LP     +     
Sbjct: 204 NNKIKGFILERGMKGLTTPKIEGKLSLRASITGQIAMDDVPVPEENLLPNAEGLQGPFGC 263

Query: 281 LAVSRVMVAWQPIGIAMGVYDMCHRYLKEREQFGAPLAAFQVNQQKLVLMLGNVQAMFLV 340
           L  +R+ +AW  +G A   + +  +Y  +R+QFG PLA  Q+ Q K+  ML  +      
Sbjct: 264 LNNARLGIAWGALGAAEECFHLARQYTLDRQQFGRPLAQNQLMQLKMADMLTEISLGLQG 323

Query: 341 GWRLCKLYEKGTMTPGQASLGKAWITLRARETVALGRELLGGNGILSDFLVAKAFCDLEP 400
             R+ +L ++G +   Q S+ K     +A E     R++LGGNGI+ ++ + +   +LE 
Sbjct: 324 CLRVSRLKDEGKVQSEQISIIKRNSCGKALEVARKARDMLGGNGIVDEYHIMRHMVNLET 383

Query: 401 IYTYEGTYDINSLVTGREITGLASF 423
           + TYEGT+D+++L+ GR ITGL  F
Sbjct: 384 VNTYEGTHDVHALILGRAITGLNGF 408

BLAST of MS016742 vs. ExPASy Swiss-Prot
Match: Q60759 (Glutaryl-CoA dehydrogenase, mitochondrial OS=Mus musculus OX=10090 GN=Gcdh PE=1 SV=2)

HSP 1 Score: 229.2 bits (583), Expect = 9.1e-59
Identity = 142/377 (37.67%), Postives = 206/377 (54.64%), Query Frame = 0

Query: 49  LTPEEQALRKKVRQ-CVEKEIAPIMTQYWEKAEFPFHVVPKLGNLCIAGGTIKGYGCPGL 108
           LT +E+ +R   R  C E+ ++ I+     +  F   +V ++G L + G TIKGYGC G+
Sbjct: 60  LTADEKLIRDTFRNYCQERLMSRILLANRNEV-FHRDIVYEMGELGVLGPTIKGYGCAGV 119

Query: 109 SITGSAVATAEVARVDASCSTFILVHSSLAMLTIALCGSEEQKHKYLPSLAKLDTIACWA 168
           S     + T E+ RVD+   + + V SSL M  I   GSEEQ+ KYLP LAK + + C+ 
Sbjct: 120 SSVAYGLLTRELERVDSGYRSMMSVQSSLVMHPIYTYGSEEQRQKYLPGLAKGELLGCFG 179

Query: 169 LTEPENGSDASGLRTTA--TKVEGGWLIEGKKRWIGNSTFADLLIIFARNTITNEINGFI 228
           LTEP +GSD  G+ T A        + + G K WI NS  ADL I++AR    N I GFI
Sbjct: 180 LTEPNHGSDPGGMETRARHNPSNQSYTLSGTKTWITNSPVADLFIVWAR-CEDNCIRGFI 239

Query: 229 MKKNAPGLVVTKIENKIGLRIVQNGDIVMNKVFVPDEDRLPGVNSFKDTNKVLAVSRVMV 288
           ++K   GL   +IE K  LR    G I+M+ V VP+E+ LP V+S       L  +R  +
Sbjct: 240 LEKGMRGLSAPRIEGKFSLRASATGMIIMDSVEVPEENVLPNVSSLAGPFGCLNTARYGI 299

Query: 289 AWQPIGIAMGVYDMCHRYLKEREQFGAPLAAFQVNQQKLVLMLGNVQAMFLVGWRLCKLY 348
            W  +G A        +Y  +R QFG PLA  Q+ Q+KL  ML  +        +L +L 
Sbjct: 300 TWGVLGAAEFCLHTARQYALDRIQFGVPLARNQLVQKKLADMLTEITLGLHACLQLGRLK 359

Query: 349 EKGTMTPGQASLGKAWITLRARETVALGRELLGGNGILSDFLVAKAFCDLEPIYTYEGTY 408
           ++   TP   S+ K     +A +     R++LGGNGI  ++ V +   +LE + TYEGT+
Sbjct: 360 DQDKATPEMVSMLKRNNCGKALDIARQARDILGGNGISDEYHVIRHAMNLEAVNTYEGTH 419

Query: 409 DINSLVTGREITGLASF 423
           DI++L+ GR ITG+ +F
Sbjct: 420 DIHALILGRAITGIQAF 434

BLAST of MS016742 vs. ExPASy Swiss-Prot
Match: Q2KHZ9 (Glutaryl-CoA dehydrogenase, mitochondrial OS=Bos taurus OX=9913 GN=GCDH PE=2 SV=1)

HSP 1 Score: 225.7 bits (574), Expect = 1.0e-57
Identity = 142/399 (35.59%), Postives = 209/399 (52.38%), Query Frame = 0

Query: 28  QATSASAFPPSVS--DYYQFNDLLTPEEQALRKKVRQCVEKEIAPIMTQYWEKAEFPFHV 87
           Q+ SA    P     D     + LT +E  +R   R   ++ + P +        F   +
Sbjct: 37  QSRSAKPSRPEFDWRDPLLLEEQLTADEILIRDTFRTYCQERLMPRILLANRNEVFHREI 96

Query: 88  VPKLGNLCIAGGTIKGYGCPGLSITGSAVATAEVARVDASCSTFILVHSSLAMLTIALCG 147
           + ++G L + G TI+GY C G+S     +   E+ RVD+   + + V SSL M  I   G
Sbjct: 97  ISEMGELGMLGPTIQGYSCAGVSSVAYGLLARELERVDSGYRSAMSVQSSLVMYPIYAYG 156

Query: 148 SEEQKHKYLPSLAKLDTIACWALTEPENGSDASGLRTTA--TKVEGGWLIEGKKRWIGNS 207
           SEEQK KYLP LAK + + C+ LTEP +GSD SG+ T A        +++ G K WI NS
Sbjct: 157 SEEQKQKYLPRLAKGELLGCFGLTEPNHGSDPSGMETRARHNPSSRSYILSGSKTWITNS 216

Query: 208 TFADLLIIFARNTITNEINGFIMKKNAPGLVVTKIENKIGLRIVQNGDIVMNKVFVPDED 267
             ADLLI++AR    + I GF+++K   GL   +IE K  LR    G I+M+ V VP+E+
Sbjct: 217 PVADLLIVWAR-CEDSCIRGFLLEKGMRGLSTPRIEGKFSLRASSTGMIIMDDVEVPEEN 276

Query: 268 RLPGVNSFKDTNKVLAVSRVMVAWQPIGIAMGVYDMCHRYLKEREQFGAPLAAFQVNQQK 327
            LPGV+        L  +R  + W  +G A        +Y  +R QFG PLA  Q+ Q+K
Sbjct: 277 VLPGVSGLAGPFGCLNNARYGITWGVLGAAEFCLHTARQYTLDRIQFGVPLAKNQLIQKK 336

Query: 328 LVLMLGNVQAMFLVGWRLCKLYEKGTMTPGQASLGKAWITLRARETVALGRELLGGNGIL 387
           L  ML  +        +L +L ++    P   SL K     +A +     R++LGGNGI 
Sbjct: 337 LADMLTEITLGLHACLQLGRLKDQDKAAPEMVSLLKRNNCGKALDIARQARDMLGGNGIS 396

Query: 388 SDFLVAKAFCDLEPIYTYEGTYDINSLVTGREITGLASF 423
            ++ V +   +LE + TYEGT+DI++L+ GR ITG+ +F
Sbjct: 397 DEYHVIRHVMNLESVNTYEGTHDIHALILGRAITGIQAF 434

BLAST of MS016742 vs. ExPASy Swiss-Prot
Match: Q54R47 (Glutaryl-CoA dehydrogenase, mitochondrial OS=Dictyostelium discoideum OX=44689 GN=gcdh PE=3 SV=1)

HSP 1 Score: 222.2 bits (565), Expect = 1.1e-56
Identity = 134/389 (34.45%), Postives = 207/389 (53.21%), Query Frame = 0

Query: 41  DYYQFND------LLTPEEQALRKKVRQCVEKEIAPIMTQYWEKAEFPFHVVPKLGNLCI 100
           D +++ND      LLT EE  +R +V +  + E+ P +   +   +F   ++ + G + +
Sbjct: 35  DKFEWNDPLSLESLLTEEEVMIRDQVNKFCQDELMPRIQMAYRDEKFDREIMREYGKMGM 94

Query: 101 AGGTIKGYGCPGLSITGSAVATAEVARVDASCSTFILVHSSLAMLTIALCGSEEQKHKYL 160
            G TI  YG  G+S     +    V +VD+   + + V SSL M  I   G++ QK KYL
Sbjct: 95  LGATIPAYG--GVSHVAYGLMANAVEKVDSGYRSAMSVQSSLVMHPINTFGTDAQKSKYL 154

Query: 161 PSLAKLDTIACWALTEPENGSDASGLRTTATK-VEGGWLIEGKKRWIGNSTFADLLIIFA 220
             LA  D + C+ LTEP  GSD +G++T A K   G +++ G K WI NS  AD+ +++A
Sbjct: 155 DGLASGDLVGCFGLTEPNAGSDPAGMQTRAVKNSAGNYVLNGTKTWITNSPIADVFVVWA 214

Query: 221 RNTITNEINGFIMKKNAPGLVVTKIENKIGLRIVQNGDIVMNKVFVPDEDRLPGVNSFKD 280
           +     +I GF+++K   GL   KIE K+ LR    G IVM  V VP     P V   + 
Sbjct: 215 K-VENGDIRGFVLEKGMKGLSAPKIEGKLSLRASITGMIVMEDVEVPPTAMFPEVKGLRG 274

Query: 281 TNKVLAVSRVMVAWQPIGIAMGVYDMCHRYLKEREQFGAPLAAFQVNQQKLVLMLGNVQA 340
               L  +R  + W  +G A   Y    +Y  +R+QFG PLAA Q+ Q+KL  M   +  
Sbjct: 275 PFSCLNKARYGIGWGSLGAAEFCYSTARQYGLDRKQFGKPLAANQLYQKKLADMATEISL 334

Query: 341 MFLVGWRLCKLYEKGTMTPGQASLGKAWITLRARETVALGRELLGGNGILSDFLVAKAFC 400
                +++ +L + G  TP + SL K     ++ +     R++LGGNGI  ++ V +   
Sbjct: 335 GLQACYQVGRLIDAGKATPERISLIKRNSCGKSLDIARQSRDMLGGNGIADEYHVIRHAA 394

Query: 401 DLEPIYTYEGTYDINSLVTGREITGLASF 423
           +LE + TYEGT+DI++L+ GR ITG+ SF
Sbjct: 395 NLETVNTYEGTHDIHALILGRAITGIPSF 420

BLAST of MS016742 vs. ExPASy TrEMBL
Match: A0A6J1CX30 (acyl-coenzyme A oxidase 4, peroxisomal OS=Momordica charantia OX=3673 GN=LOC111015009 PE=3 SV=1)

HSP 1 Score: 862.4 bits (2227), Expect = 7.8e-247
Identity = 432/435 (99.31%), Postives = 432/435 (99.31%), Query Frame = 0

Query: 1   NIADDNDKNSRSSYFGLPALDVSVAFPQATSASAFPPSVSDYYQFNDLLTPEEQALRKKV 60
           N  DDNDKNSRSSYFGLPALDVSVAFPQATSASAFPPSVSDYYQFN LLTPEEQALRKKV
Sbjct: 2   NRQDDNDKNSRSSYFGLPALDVSVAFPQATSASAFPPSVSDYYQFNGLLTPEEQALRKKV 61

Query: 61  RQCVEKEIAPIMTQYWEKAEFPFHVVPKLGNLCIAGGTIKGYGCPGLSITGSAVATAEVA 120
           RQCVEKEIAPIMTQYWEKAEFPFHVVPKLGNLCIAGGTIKGYGCPGLSITGSAVATAEVA
Sbjct: 62  RQCVEKEIAPIMTQYWEKAEFPFHVVPKLGNLCIAGGTIKGYGCPGLSITGSAVATAEVA 121

Query: 121 RVDASCSTFILVHSSLAMLTIALCGSEEQKHKYLPSLAKLDTIACWALTEPENGSDASGL 180
           RVDASCSTFILVHSSLAMLTIALCGSEEQKHKYLPSLAKLDTIACWALTEPENGSDASGL
Sbjct: 122 RVDASCSTFILVHSSLAMLTIALCGSEEQKHKYLPSLAKLDTIACWALTEPENGSDASGL 181

Query: 181 RTTATKVEGGWLIEGKKRWIGNSTFADLLIIFARNTITNEINGFIMKKNAPGLVVTKIEN 240
           RTTATKVEGGWLIEGKKRWIGNSTFADLLIIFARNTITNEINGFIMKKNAPGLVVTKIEN
Sbjct: 182 RTTATKVEGGWLIEGKKRWIGNSTFADLLIIFARNTITNEINGFIMKKNAPGLVVTKIEN 241

Query: 241 KIGLRIVQNGDIVMNKVFVPDEDRLPGVNSFKDTNKVLAVSRVMVAWQPIGIAMGVYDMC 300
           KIGLRIVQNGDIVMNKVFVPDEDRLPGVNSFKDTNKVLAVSRVMVAWQPIGIAMGVYDMC
Sbjct: 242 KIGLRIVQNGDIVMNKVFVPDEDRLPGVNSFKDTNKVLAVSRVMVAWQPIGIAMGVYDMC 301

Query: 301 HRYLKEREQFGAPLAAFQVNQQKLVLMLGNVQAMFLVGWRLCKLYEKGTMTPGQASLGKA 360
           HRYLKEREQFGAPLAAFQVNQQKLVLMLGNVQAMFLVGWRLCKLYEKGTMTPGQASLGKA
Sbjct: 302 HRYLKEREQFGAPLAAFQVNQQKLVLMLGNVQAMFLVGWRLCKLYEKGTMTPGQASLGKA 361

Query: 361 WITLRARETVALGRELLGGNGILSDFLVAKAFCDLEPIYTYEGTYDINSLVTGREITGLA 420
           WITLRARETVALGRELLGGNGILSDFLVAKAFCDLEPIYTYEGTYDINSLVTGREITGLA
Sbjct: 362 WITLRARETVALGRELLGGNGILSDFLVAKAFCDLEPIYTYEGTYDINSLVTGREITGLA 421

Query: 421 SFKPAAAALTKRSRL 436
           SFKPAAAALTKRSRL
Sbjct: 422 SFKPAAAALTKRSRL 436

BLAST of MS016742 vs. ExPASy TrEMBL
Match: A0A6J1F2Z7 (acyl-coenzyme A oxidase 4, peroxisomal OS=Cucurbita moschata OX=3662 GN=LOC111441670 PE=3 SV=1)

HSP 1 Score: 827.8 bits (2137), Expect = 2.1e-236
Identity = 409/435 (94.02%), Postives = 426/435 (97.93%), Query Frame = 0

Query: 1   NIADDNDKNSRSSYFGLPALDVSVAFPQATSASAFPPSVSDYYQFNDLLTPEEQALRKKV 60
           N  DD DKNSRS+YFG PALDVSVAFPQAT+AS FPPSVSDYYQFNDLLTPEEQALRKKV
Sbjct: 8   NREDDKDKNSRSAYFGSPALDVSVAFPQATAASVFPPSVSDYYQFNDLLTPEEQALRKKV 67

Query: 61  RQCVEKEIAPIMTQYWEKAEFPFHVVPKLGNLCIAGGTIKGYGCPGLSITGSAVATAEVA 120
           RQC+EKEIAPIMT+YWEKAEFPFHV+PKLG+LCIAGGTIKGYGCPG++ITGSA+ATAEVA
Sbjct: 68  RQCMEKEIAPIMTKYWEKAEFPFHVIPKLGDLCIAGGTIKGYGCPGMTITGSAIATAEVA 127

Query: 121 RVDASCSTFILVHSSLAMLTIALCGSEEQKHKYLPSLAKLDTIACWALTEPENGSDASGL 180
           RVDASCSTFILVHSSLAMLTIALCGSEEQK KYLPSLAKLDTIACWALTEPENGSDASGL
Sbjct: 128 RVDASCSTFILVHSSLAMLTIALCGSEEQKQKYLPSLAKLDTIACWALTEPENGSDASGL 187

Query: 181 RTTATKVEGGWLIEGKKRWIGNSTFADLLIIFARNTITNEINGFIMKKNAPGLVVTKIEN 240
           RTTATKVEGGWLIEGKKRWIGNSTFADLL+IFARNTITNEINGFI+KKN+PGL+VTKIEN
Sbjct: 188 RTTATKVEGGWLIEGKKRWIGNSTFADLLVIFARNTITNEINGFIIKKNSPGLMVTKIEN 247

Query: 241 KIGLRIVQNGDIVMNKVFVPDEDRLPGVNSFKDTNKVLAVSRVMVAWQPIGIAMGVYDMC 300
           KIGLRIVQNGDIVMNKVFVPDEDRLPGVNSFKDTNKVLAVSRVMVAWQPIGIAMGVYDMC
Sbjct: 248 KIGLRIVQNGDIVMNKVFVPDEDRLPGVNSFKDTNKVLAVSRVMVAWQPIGIAMGVYDMC 307

Query: 301 HRYLKEREQFGAPLAAFQVNQQKLVLMLGNVQAMFLVGWRLCKLYEKGTMTPGQASLGKA 360
           HRYLKEREQFGAPLAAFQVNQQKLVLMLGNVQAMFL+GWRLCKLYEKGTMTPGQASLGKA
Sbjct: 308 HRYLKEREQFGAPLAAFQVNQQKLVLMLGNVQAMFLMGWRLCKLYEKGTMTPGQASLGKA 367

Query: 361 WITLRARETVALGRELLGGNGILSDFLVAKAFCDLEPIYTYEGTYDINSLVTGREITGLA 420
           WITLRARETVALGRELLGGNGILSDFLVAKAFCDLEPIYTYEGTYDIN+LVTGREITG+A
Sbjct: 368 WITLRARETVALGRELLGGNGILSDFLVAKAFCDLEPIYTYEGTYDINTLVTGREITGVA 427

Query: 421 SFKPAAAALTKRSRL 436
           SFKP  A+L KRSRL
Sbjct: 428 SFKP--ASLAKRSRL 440

BLAST of MS016742 vs. ExPASy TrEMBL
Match: A0A5D3DNU3 (Acyl-coenzyme A oxidase 4, peroxisomal OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G00660 PE=3 SV=1)

HSP 1 Score: 826.6 bits (2134), Expect = 4.7e-236
Identity = 408/435 (93.79%), Postives = 425/435 (97.70%), Query Frame = 0

Query: 1   NIADDNDKNSRSSYFGLPALDVSVAFPQATSASAFPPSVSDYYQFNDLLTPEEQALRKKV 60
           N  DD DKN+RS+YFGLPALDVSVAFPQATSAS+FPPSVSD+YQFNDLLTPEEQALRKKV
Sbjct: 8   NRQDDKDKNARSAYFGLPALDVSVAFPQATSASSFPPSVSDFYQFNDLLTPEEQALRKKV 67

Query: 61  RQCVEKEIAPIMTQYWEKAEFPFHVVPKLGNLCIAGGTIKGYGCPGLSITGSAVATAEVA 120
           RQCVEKE+APIMT+YWEKAEFPF VVPKLG+LCIAGGTIKGYGCPGLSITGSAVA AEVA
Sbjct: 68  RQCVEKEVAPIMTKYWEKAEFPFEVVPKLGSLCIAGGTIKGYGCPGLSITGSAVAVAEVA 127

Query: 121 RVDASCSTFILVHSSLAMLTIALCGSEEQKHKYLPSLAKLDTIACWALTEPENGSDASGL 180
           RVDASCSTFILVHSSLAMLTIALCGSEEQKHKYLPSLAK DT+ACWALTEPENGSDASGL
Sbjct: 128 RVDASCSTFILVHSSLAMLTIALCGSEEQKHKYLPSLAKFDTVACWALTEPENGSDASGL 187

Query: 181 RTTATKVEGGWLIEGKKRWIGNSTFADLLIIFARNTITNEINGFIMKKNAPGLVVTKIEN 240
           RTTATKVEGGW+IEGKKRWIGNSTFAD+L+IFARNTITNEINGFI+KKNAPGL VTKIEN
Sbjct: 188 RTTATKVEGGWVIEGKKRWIGNSTFADVLVIFARNTITNEINGFIIKKNAPGLTVTKIEN 247

Query: 241 KIGLRIVQNGDIVMNKVFVPDEDRLPGVNSFKDTNKVLAVSRVMVAWQPIGIAMGVYDMC 300
           KIGLRIVQNGDIVMNKVFVPDEDRLPGVNSFKDTNKVLAVSRVMVAWQPIGIAMGVYDMC
Sbjct: 248 KIGLRIVQNGDIVMNKVFVPDEDRLPGVNSFKDTNKVLAVSRVMVAWQPIGIAMGVYDMC 307

Query: 301 HRYLKEREQFGAPLAAFQVNQQKLVLMLGNVQAMFLVGWRLCKLYEKGTMTPGQASLGKA 360
           HRYLKEREQFGAPLAAFQ+NQQKLVLMLGNVQAMFL+GWRLCKLYEKGTMTPGQASLGKA
Sbjct: 308 HRYLKEREQFGAPLAAFQLNQQKLVLMLGNVQAMFLIGWRLCKLYEKGTMTPGQASLGKA 367

Query: 361 WITLRARETVALGRELLGGNGILSDFLVAKAFCDLEPIYTYEGTYDINSLVTGREITGLA 420
           WITLRARETV+LGRELLGGNGILSDFLVAKAFCDLEPIYTYEGTYDIN+LVTGREITG+A
Sbjct: 368 WITLRARETVSLGRELLGGNGILSDFLVAKAFCDLEPIYTYEGTYDINTLVTGREITGVA 427

Query: 421 SFKPAAAALTKRSRL 436
           SFKP  AAL KRSRL
Sbjct: 428 SFKP--AALAKRSRL 440

BLAST of MS016742 vs. ExPASy TrEMBL
Match: A0A6J1IZ14 (acyl-coenzyme A oxidase 4, peroxisomal OS=Cucurbita maxima OX=3661 GN=LOC111481955 PE=3 SV=1)

HSP 1 Score: 826.6 bits (2134), Expect = 4.7e-236
Identity = 408/435 (93.79%), Postives = 424/435 (97.47%), Query Frame = 0

Query: 1   NIADDNDKNSRSSYFGLPALDVSVAFPQATSASAFPPSVSDYYQFNDLLTPEEQALRKKV 60
           N  DD DKNSRS+YFG PALDVSVAFPQAT+AS FPPSVSDYYQFNDLL PEEQALRKKV
Sbjct: 8   NREDDKDKNSRSAYFGSPALDVSVAFPQATAASVFPPSVSDYYQFNDLLIPEEQALRKKV 67

Query: 61  RQCVEKEIAPIMTQYWEKAEFPFHVVPKLGNLCIAGGTIKGYGCPGLSITGSAVATAEVA 120
           RQC+EKEIAPIMT+YWEKAEFPFH++PKLGNLCIAGGTIKGYGCPG++ITG A+ATAEVA
Sbjct: 68  RQCMEKEIAPIMTKYWEKAEFPFHIIPKLGNLCIAGGTIKGYGCPGMTITGCAIATAEVA 127

Query: 121 RVDASCSTFILVHSSLAMLTIALCGSEEQKHKYLPSLAKLDTIACWALTEPENGSDASGL 180
           RVDASCSTFILVHSSLAMLT+ALCGSEEQK KYLPSLAKLDTIACWALTEPENGSDASGL
Sbjct: 128 RVDASCSTFILVHSSLAMLTMALCGSEEQKQKYLPSLAKLDTIACWALTEPENGSDASGL 187

Query: 181 RTTATKVEGGWLIEGKKRWIGNSTFADLLIIFARNTITNEINGFIMKKNAPGLVVTKIEN 240
           RTTATKVEGGWLIEGKKRWIGNSTFADLL+IFARNTITNEINGFIMKKN+PGL+VTKIEN
Sbjct: 188 RTTATKVEGGWLIEGKKRWIGNSTFADLLVIFARNTITNEINGFIMKKNSPGLMVTKIEN 247

Query: 241 KIGLRIVQNGDIVMNKVFVPDEDRLPGVNSFKDTNKVLAVSRVMVAWQPIGIAMGVYDMC 300
           KIGLRIVQNGDIVMNKVFVPDEDRLPGVNSFKDTNKVLAVSRVMVAWQPIGIAMGVYDMC
Sbjct: 248 KIGLRIVQNGDIVMNKVFVPDEDRLPGVNSFKDTNKVLAVSRVMVAWQPIGIAMGVYDMC 307

Query: 301 HRYLKEREQFGAPLAAFQVNQQKLVLMLGNVQAMFLVGWRLCKLYEKGTMTPGQASLGKA 360
           HRYLKEREQFGAPLAAFQVNQQKLVLMLGNVQAMFLVGWRLCKLYEKGTMTPGQASLGKA
Sbjct: 308 HRYLKEREQFGAPLAAFQVNQQKLVLMLGNVQAMFLVGWRLCKLYEKGTMTPGQASLGKA 367

Query: 361 WITLRARETVALGRELLGGNGILSDFLVAKAFCDLEPIYTYEGTYDINSLVTGREITGLA 420
           WITLRARETVALGRELLGGNGILSDFLVAKAFCDLEPIYTYEGTYDIN+LVTGREITG+A
Sbjct: 368 WITLRARETVALGRELLGGNGILSDFLVAKAFCDLEPIYTYEGTYDINTLVTGREITGVA 427

Query: 421 SFKPAAAALTKRSRL 436
           SFKP  A+L KRSRL
Sbjct: 428 SFKP--ASLAKRSRL 440

BLAST of MS016742 vs. ExPASy TrEMBL
Match: A0A1S3B696 (acyl-coenzyme A oxidase 4, peroxisomal OS=Cucumis melo OX=3656 GN=LOC103486279 PE=3 SV=1)

HSP 1 Score: 826.6 bits (2134), Expect = 4.7e-236
Identity = 408/435 (93.79%), Postives = 425/435 (97.70%), Query Frame = 0

Query: 1   NIADDNDKNSRSSYFGLPALDVSVAFPQATSASAFPPSVSDYYQFNDLLTPEEQALRKKV 60
           N  DD DKN+RS+YFGLPALDVSVAFPQATSAS+FPPSVSD+YQFNDLLTPEEQALRKKV
Sbjct: 8   NRQDDKDKNARSAYFGLPALDVSVAFPQATSASSFPPSVSDFYQFNDLLTPEEQALRKKV 67

Query: 61  RQCVEKEIAPIMTQYWEKAEFPFHVVPKLGNLCIAGGTIKGYGCPGLSITGSAVATAEVA 120
           RQCVEKE+APIMT+YWEKAEFPF VVPKLG+LCIAGGTIKGYGCPGLSITGSAVA AEVA
Sbjct: 68  RQCVEKEVAPIMTKYWEKAEFPFEVVPKLGSLCIAGGTIKGYGCPGLSITGSAVAVAEVA 127

Query: 121 RVDASCSTFILVHSSLAMLTIALCGSEEQKHKYLPSLAKLDTIACWALTEPENGSDASGL 180
           RVDASCSTFILVHSSLAMLTIALCGSEEQKHKYLPSLAK DT+ACWALTEPENGSDASGL
Sbjct: 128 RVDASCSTFILVHSSLAMLTIALCGSEEQKHKYLPSLAKFDTVACWALTEPENGSDASGL 187

Query: 181 RTTATKVEGGWLIEGKKRWIGNSTFADLLIIFARNTITNEINGFIMKKNAPGLVVTKIEN 240
           RTTATKVEGGW+IEGKKRWIGNSTFAD+L+IFARNTITNEINGFI+KKNAPGL VTKIEN
Sbjct: 188 RTTATKVEGGWVIEGKKRWIGNSTFADVLVIFARNTITNEINGFIIKKNAPGLTVTKIEN 247

Query: 241 KIGLRIVQNGDIVMNKVFVPDEDRLPGVNSFKDTNKVLAVSRVMVAWQPIGIAMGVYDMC 300
           KIGLRIVQNGDIVMNKVFVPDEDRLPGVNSFKDTNKVLAVSRVMVAWQPIGIAMGVYDMC
Sbjct: 248 KIGLRIVQNGDIVMNKVFVPDEDRLPGVNSFKDTNKVLAVSRVMVAWQPIGIAMGVYDMC 307

Query: 301 HRYLKEREQFGAPLAAFQVNQQKLVLMLGNVQAMFLVGWRLCKLYEKGTMTPGQASLGKA 360
           HRYLKEREQFGAPLAAFQ+NQQKLVLMLGNVQAMFL+GWRLCKLYEKGTMTPGQASLGKA
Sbjct: 308 HRYLKEREQFGAPLAAFQLNQQKLVLMLGNVQAMFLIGWRLCKLYEKGTMTPGQASLGKA 367

Query: 361 WITLRARETVALGRELLGGNGILSDFLVAKAFCDLEPIYTYEGTYDINSLVTGREITGLA 420
           WITLRARETV+LGRELLGGNGILSDFLVAKAFCDLEPIYTYEGTYDIN+LVTGREITG+A
Sbjct: 368 WITLRARETVSLGRELLGGNGILSDFLVAKAFCDLEPIYTYEGTYDINTLVTGREITGVA 427

Query: 421 SFKPAAAALTKRSRL 436
           SFKP  AAL KRSRL
Sbjct: 428 SFKP--AALAKRSRL 440

BLAST of MS016742 vs. TAIR 10
Match: AT3G51840.1 (acyl-CoA oxidase 4 )

HSP 1 Score: 731.1 bits (1886), Expect = 5.2e-211
Identity = 352/430 (81.86%), Postives = 394/430 (91.63%), Query Frame = 0

Query: 6   NDKNSRSSYFGLPALDVSVAFPQATSASAFPPSVSDYYQFNDLLTPEEQALRKKVRQCVE 65
           N+K  +SSYF LP +++SVAFPQAT AS FPP  SDYY FNDLLTPEEQA+RKKVR+C+E
Sbjct: 12  NEKKVKSSYFDLPPMEMSVAFPQATPASTFPPCTSDYYHFNDLLTPEEQAIRKKVRECME 71

Query: 66  KEIAPIMTQYWEKAEFPFHVVPKLGNLCIAGGTIKGYGCPGLSITGSAVATAEVARVDAS 125
           KE+APIMT+YWEKAEFPFH+ PKLG + +AGG+IKGYGCPGLSIT +A+ATAE+ARVDAS
Sbjct: 72  KEVAPIMTEYWEKAEFPFHITPKLGAMGVAGGSIKGYGCPGLSITANAIATAEIARVDAS 131

Query: 126 CSTFILVHSSLAMLTIALCGSEEQKHKYLPSLAKLDTIACWALTEPENGSDASGLRTTAT 185
           CSTFILVHSSL MLTIALCGSE QK KYLPSLA+L+T+ACWALTEP+NGSDASGL TTAT
Sbjct: 132 CSTFILVHSSLGMLTIALCGSEAQKEKYLPSLAQLNTVACWALTEPDNGSDASGLGTTAT 191

Query: 186 KVEGGWLIEGKKRWIGNSTFADLLIIFARNTITNEINGFIMKKNAPGLVVTKIENKIGLR 245
           KVEGGW I G+KRWIGNSTFADLLIIFARNT TN+INGFI+KK+APGL  TKI NKIGLR
Sbjct: 192 KVEGGWKINGQKRWIGNSTFADLLIIFARNTTTNQINGFIVKKDAPGLKATKIPNKIGLR 251

Query: 246 IVQNGDIVMNKVFVPDEDRLPGVNSFKDTNKVLAVSRVMVAWQPIGIAMGVYDMCHRYLK 305
           +VQNGDI++  VFVPDEDRLPGVNSF+DT+KVLAVSRVMVAWQPIGI+MG+YDMCHRYLK
Sbjct: 252 MVQNGDILLQNVFVPDEDRLPGVNSFQDTSKVLAVSRVMVAWQPIGISMGIYDMCHRYLK 311

Query: 306 EREQFGAPLAAFQVNQQKLVLMLGNVQAMFLVGWRLCKLYEKGTMTPGQASLGKAWITLR 365
           ER+QFGAPLAAFQ+NQQKLV MLGNVQAMFL+GWRLCKLYE G MTPGQASLGKAWI+ +
Sbjct: 312 ERKQFGAPLAAFQLNQQKLVQMLGNVQAMFLMGWRLCKLYETGQMTPGQASLGKAWISSK 371

Query: 366 ARETVALGRELLGGNGILSDFLVAKAFCDLEPIYTYEGTYDINSLVTGREITGLASFKPA 425
           ARET +LGRELLGGNGIL+DFLVAKAFCDLEPIYTYEGTYDIN+LVTGRE+TG+ASFKPA
Sbjct: 372 ARETASLGRELLGGNGILADFLVAKAFCDLEPIYTYEGTYDINTLVTGREVTGIASFKPA 431

Query: 426 AAALTKRSRL 436
                 RSRL
Sbjct: 432 T-----RSRL 436

BLAST of MS016742 vs. TAIR 10
Match: AT3G45300.1 (isovaleryl-CoA-dehydrogenase )

HSP 1 Score: 144.1 bits (362), Expect = 2.7e-34
Identity = 100/370 (27.03%), Postives = 174/370 (47.03%), Query Frame = 0

Query: 57  RKKVRQCVEKEIAPIMTQYWEKAEFP--FHVVPKLGNLCIAGGTI-KGYGCPGLSITGSA 116
           ++ V +  +  IAP   +  +   FP   ++   +G   + G T  + YG  GL      
Sbjct: 39  KESVSKFAQDNIAPHAERIDKTNSFPKDVNLWKLMGEFNLHGITAPEEYGGLGLGYLYHC 98

Query: 117 VATAEVARVDASCSTFILVHSSLAMLTIALCGSEEQKHKYLPSLAKLDTIACWALTEPEN 176
           +A  E++R   S +     HS+L +  +   G+  QK KYLP L   + +   A++EP  
Sbjct: 99  IAMEEISRASGSVALSYGAHSNLCINQLVRNGTAAQKEKYLPKLISGEHVGALAMSEPNA 158

Query: 177 GSDASGLRTTATKVEGGWLIEGKKRWIGNSTFADLLIIFARNTI---TNEINGFIMKKNA 236
           GSD  G++  A KV+GG+++ G K W  N   A+ L+++A+      +  I  FI++K  
Sbjct: 159 GSDVVGMKCKAEKVDGGYILNGNKMWCTNGPSAETLVVYAKTDTKAGSKGITAFIIEKGM 218

Query: 237 PGLVVTKIENKIGLRIVQNGDIVMNKVFVPDEDRL----PGVNSFKDTNKVLAVSRVMVA 296
            G    +  +K+G+R     ++V    FVP+E+ L     GV         L + R+++A
Sbjct: 219 TGFSTAQKLDKLGMRGSDTCELVFENCFVPEENILDKEGKGVYVLMSG---LDLERLVLA 278

Query: 297 WQPIGIAMGVYDMCHRYLKEREQFGAPLAAFQVNQQKLVLMLGNVQAMFLVGWRLCKLYE 356
             P+GI     D    Y+++REQFG P+  FQ  Q K+  M   +Q+     + + +  +
Sbjct: 279 AGPLGIMQACLDNVLPYIRQREQFGRPVGEFQFIQGKVADMYTALQSSRSYVYSVARDCD 338

Query: 357 KGTMTPGQASLGKAWITLRARETVALGRELLGGNGILSDFLVAKAFCDLEPIYTYEGTYD 416
            G + P   +        RA +      + LGGNG ++++   +   D +      GT +
Sbjct: 339 NGKVDPKDCAGTILCAAERATQVALQAIQCLGGNGYINEYATGRLLRDAKLYEIGAGTSE 398

BLAST of MS016742 vs. TAIR 10
Match: AT2G35690.1 (acyl-CoA oxidase 5 )

HSP 1 Score: 64.3 bits (155), Expect = 2.7e-10
Identity = 81/339 (23.89%), Postives = 134/339 (39.53%), Query Frame = 0

Query: 129 FILVHSSLAMLTIALCGSEEQKHKYLPSLAKLDTIACWALTEPENGSDASGLRTTAT--K 188
           F+ +H  + +  I   G+E+Q+ K+L    K+  I C+A TE  +GS+  GL TTAT   
Sbjct: 98  FLDLHWGMFVPAIKGQGTEQQQQKWLSLATKMQIIGCYAQTELGHGSNVQGLETTATFDP 157

Query: 189 VEGGWLIE-----GKKRWIGN----STFADLLIIFARNTITNEINGFIMKKNA------- 248
               ++I        K W G     ST A +      N   + ++GFI++  +       
Sbjct: 158 KTDQFIIHSPTQTSSKWWPGGLGKVSTHAVIYARLITNGKDHGVHGFIVQLRSLDDHSPL 217

Query: 249 PGLVVTKIENKIG---LRIVQNGDIVMNKVFVPDEDRLPGVNSFKDTNKVLA--VSRVMV 308
           PG+ V  I  K G      + NG ++ +   +P +  L  ++      K +A  V R +V
Sbjct: 218 PGITVGDIGMKFGNGAYNSMDNGFLMFDHFRIPRDQMLMRLSKVTREGKYVASDVPRQLV 277

Query: 309 AWQPIGIAMGVYD-----------MCHRYLKEREQFGA-------PLAAFQVNQQKLVLM 368
               + +   +             +  RY   R QFG+        +  ++  Q +L  +
Sbjct: 278 YGTMVYVRQSIVSNASTALARAVCIATRYSAVRRQFGSHDGGIETQVINYKTQQNRLFPL 337

Query: 369 LGNVQAMFLVGWRLCKLY----------EKGTMTPGQASLG--KAWITLRARETVALGRE 415
           L +  A   VG  L  LY          +  T+    A     K+  T    + +   R+
Sbjct: 338 LASAYAFRFVGEWLKWLYTDVTKRLEASDFATLPEAHACTAGLKSMTTSATSDGIEECRK 397

BLAST of MS016742 vs. TAIR 10
Match: AT4G16760.1 (acyl-CoA oxidase 1 )

HSP 1 Score: 61.6 bits (148), Expect = 1.8e-09
Identity = 87/343 (25.36%), Postives = 137/343 (39.94%), Query Frame = 0

Query: 129 FILVHSSLAMLTIALCGSEEQKHKYLPSLAKLDTIACWALTEPENGSDASGLRTTAT--K 188
           ++ +H  + +  I   G+EEQ+ K+L    K+  I C+A TE  +GS+  GL TTAT   
Sbjct: 98  YVDLHWGMFVPAIKGQGTEEQQKKWLSLANKMQIIGCYAQTELGHGSNVQGLETTATFDP 157

Query: 189 VEGGWLIE-----GKKRWIGN----STFADLLIIFARNTITN----EINGFIMKKNA--- 248
               ++I        K W G     ST A   +++AR  ITN     I+GFI++  +   
Sbjct: 158 KTDEFVIHTPTQTASKWWPGGLGKVSTHA---VVYAR-LITNGKDYGIHGFIVQLRSLED 217

Query: 249 ----PGLVVTKIENKIG---LRIVQNGDIVMNKVFVPDEDRLPGVNSF--------KDTN 308
               P + V  I  K+G      + NG ++ + V +P +  L  ++           D  
Sbjct: 218 HSPLPNITVGDIGTKMGNGAYNSMDNGFLMFDHVRIPRDQMLMRLSKVTREGEYVPSDVP 277

Query: 309 KVLAVSRVMVAWQPI-----GIAMGVYDMCHRYLKEREQFGA-------PLAAFQVNQQK 368
           K L    ++   Q I             +  RY   R QFGA        +  ++  Q +
Sbjct: 278 KQLVYGTMVYVRQTIVADASNALSRAVCIATRYSAVRRQFGAHNGGIETQVIDYKTQQNR 337

Query: 369 LVLMLGNVQAMFLVGWRLCKLY----------EKGTMTPGQASLG--KAWITLRARETVA 415
           L  +L +  A   VG  L  LY          +  T+    A     K+  T    + + 
Sbjct: 338 LFPLLASAYAFRFVGEWLKWLYTDVTERLAASDFATLPEAHACTAGLKSLTTTATADGIE 397

BLAST of MS016742 vs. TAIR 10
Match: AT4G16760.2 (acyl-CoA oxidase 1 )

HSP 1 Score: 61.6 bits (148), Expect = 1.8e-09
Identity = 87/343 (25.36%), Postives = 137/343 (39.94%), Query Frame = 0

Query: 129 FILVHSSLAMLTIALCGSEEQKHKYLPSLAKLDTIACWALTEPENGSDASGLRTTAT--K 188
           ++ +H  + +  I   G+EEQ+ K+L    K+  I C+A TE  +GS+  GL TTAT   
Sbjct: 85  YVDLHWGMFVPAIKGQGTEEQQKKWLSLANKMQIIGCYAQTELGHGSNVQGLETTATFDP 144

Query: 189 VEGGWLIE-----GKKRWIGN----STFADLLIIFARNTITN----EINGFIMKKNA--- 248
               ++I        K W G     ST A   +++AR  ITN     I+GFI++  +   
Sbjct: 145 KTDEFVIHTPTQTASKWWPGGLGKVSTHA---VVYAR-LITNGKDYGIHGFIVQLRSLED 204

Query: 249 ----PGLVVTKIENKIG---LRIVQNGDIVMNKVFVPDEDRLPGVNSF--------KDTN 308
               P + V  I  K+G      + NG ++ + V +P +  L  ++           D  
Sbjct: 205 HSPLPNITVGDIGTKMGNGAYNSMDNGFLMFDHVRIPRDQMLMRLSKVTREGEYVPSDVP 264

Query: 309 KVLAVSRVMVAWQPI-----GIAMGVYDMCHRYLKEREQFGA-------PLAAFQVNQQK 368
           K L    ++   Q I             +  RY   R QFGA        +  ++  Q +
Sbjct: 265 KQLVYGTMVYVRQTIVADASNALSRAVCIATRYSAVRRQFGAHNGGIETQVIDYKTQQNR 324

Query: 369 LVLMLGNVQAMFLVGWRLCKLY----------EKGTMTPGQASLG--KAWITLRARETVA 415
           L  +L +  A   VG  L  LY          +  T+    A     K+  T    + + 
Sbjct: 325 LFPLLASAYAFRFVGEWLKWLYTDVTERLAASDFATLPEAHACTAGLKSLTTTATADGIE 384