MS016738 (gene) Bitter gourd (TR) v1

Overview


Name	MS016738
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	VARLMGL domain-containing protein
Location	scaffold563: 541570 .. 542713 (-)
RNA-Seq Expression	MS016738
Synteny	MS016738

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCCCAACTCCGGCCAACCCAATCCCGGCTGCTTTTCCGGCATGCTGCGGCGGCTCTTTTGCACCGGCAATCTCCCCACTCACCCATCCGACGCCCTGAACGAACCACAGTTAGAAAAGGCCGCCGAGGCTAAACCCGGGCCGGGCGTGGTGGCTCGGTTGATGGGCTTGAGTTCGCTGCCGGACGCCAATTGGGTCCCGAACCACCGAGCCCCGGGTGCGGTCCCGCGCAGCAAATCCGTGAACTTCGCGGATTATCTGCTGCACTTTGACTCGAGTCAAGCCCACCACCGCCGAGTCCGAACCTCCGCGTCGTTTCGTGAGGTCCCACCGTTGAATCCACACAACGATTTCTTGGTTCTGTACACGAAGGATTATTTCGACGGCTACGAAGCGGAAATTGAGTCCAATTTGAAGGAACCCGAAACGCACCGTTTGAGAGAAGCTAAACAGGGGAAGGAATTGCAGAGCAGTAATGTGAAGAAGAAGAAGAAGAATTCGAGAAATGAATTCAAAATTTCGAAGCTGAAAAACGAGCCAAGAAGGGAAGTTGGTAAGGATTTCTCCAAGAAGAACAGAAAATGCAGCTCCAATAAAGATTCGGTCTCTGTTTTACCGTCGTGTAAATATAAACAGAGTGTTACTAAAAAACTTGGCGCTGCGAATTTGAAATCTCCGCAGAAGAAGCCACCGAAACAGAAGGAAGTAGCCATTGGAACAGAGGTAAAAAAGAAGATAAAGAATAATTCTCTTCGACATGTCGGAACGAAACCCGACCCGGAATCCGACCCGGACAATTCCAGCCCGATTTCGGTCCTCGATGACGGCGGATTCGATTTCTCGGGTAATACTTCTAAATCCAGAGTAATTAATTATTATTATTACCTTTTTTTTTAACGCGTGAATTTCTGCAGATGAGCGGAGCTCGCCGAAGACGGAGGGGGGATTAGAGAAATGTCAGACCGGCGGCGACCGGAGCGAGGAGGAGGTGGGAAGGAGCTGCAGGTTGGCAGATGAAGATATAAGAGAGGCGAATTGGAGTGTGAAGAATGTGTGGGAATATGAAGCGTTAGAAGAATTATGCATGGAGCTTGAACGACATGTCGTCAATGTATTGTTGGTTCAGACCCTAGATGACCTTGTG

mRNA sequence

ATGCCCAACTCCGGCCAACCCAATCCCGGCTGCTTTTCCGGCATGCTGCGGCGGCTCTTTTGCACCGGCAATCTCCCCACTCACCCATCCGACGCCCTGAACGAACCACAGTTAGAAAAGGCCGCCGAGGCTAAACCCGGGCCGGGCGTGGTGGCTCGGTTGATGGGCTTGAGTTCGCTGCCGGACGCCAATTGGGTCCCGAACCACCGAGCCCCGGGTGCGGTCCCGCGCAGCAAATCCGTGAACTTCGCGGATTATCTGCTGCACTTTGACTCGAGTCAAGCCCACCACCGCCGAGTCCGAACCTCCGCGTCGTTTCGTGAGGTCCCACCGTTGAATCCACACAACGATTTCTTGGTTCTGTACACGAAGGATTATTTCGACGGCTACGAAGCGGAAATTGAGTCCAATTTGAAGGAACCCGAAACGCACCGTTTGAGAGAAGCTAAACAGGGGAAGGAATTGCAGAGCAGTAATGTGAAGAAGAAGAAGAAGAATTCGAGAAATGAATTCAAAATTTCGAAGCTGAAAAACGAGCCAAGAAGGGAAGTTGGTAAGGATTTCTCCAAGAAGAACAGAAAATGCAGCTCCAATAAAGATTCGGTCTCTGTTTTACCGTCGTGTAAATATAAACAGAGTGTTACTAAAAAACTTGGCGCTGCGAATTTGAAATCTCCGCAGAAGAAGCCACCGAAACAGAAGGAAGTAGCCATTGGAACAGAGGTAAAAAAGAAGATAAAGAATAATTCTCTTCGACATGTCGGAACGAAACCCGACCCGGAATCCGACCCGGACAATTCCAGCCCGATTTCGGTCCTCGATGACGGCGGATTCGATTTCTCGGAGAAATGTCAGACCGGCGGCGACCGGAGCGAGGAGGAGGTGGGAAGGAGCTGCAGGTTGGCAGATGAAGATATAAGAGAGGCGAATTGGAGTGTGAAGAATGTGTGGGAATATGAAGCGTTAGAAGAATTATGCATGGAGCTTGAACGACATGTCGTCAATGTATTGTTGGTTCAGACCCTAGATGACCTTGTG

Coding sequence (CDS)

ATGCCCAACTCCGGCCAACCCAATCCCGGCTGCTTTTCCGGCATGCTGCGGCGGCTCTTTTGCACCGGCAATCTCCCCACTCACCCATCCGACGCCCTGAACGAACCACAGTTAGAAAAGGCCGCCGAGGCTAAACCCGGGCCGGGCGTGGTGGCTCGGTTGATGGGCTTGAGTTCGCTGCCGGACGCCAATTGGGTCCCGAACCACCGAGCCCCGGGTGCGGTCCCGCGCAGCAAATCCGTGAACTTCGCGGATTATCTGCTGCACTTTGACTCGAGTCAAGCCCACCACCGCCGAGTCCGAACCTCCGCGTCGTTTCGTGAGGTCCCACCGTTGAATCCACACAACGATTTCTTGGTTCTGTACACGAAGGATTATTTCGACGGCTACGAAGCGGAAATTGAGTCCAATTTGAAGGAACCCGAAACGCACCGTTTGAGAGAAGCTAAACAGGGGAAGGAATTGCAGAGCAGTAATGTGAAGAAGAAGAAGAAGAATTCGAGAAATGAATTCAAAATTTCGAAGCTGAAAAACGAGCCAAGAAGGGAAGTTGGTAAGGATTTCTCCAAGAAGAACAGAAAATGCAGCTCCAATAAAGATTCGGTCTCTGTTTTACCGTCGTGTAAATATAAACAGAGTGTTACTAAAAAACTTGGCGCTGCGAATTTGAAATCTCCGCAGAAGAAGCCACCGAAACAGAAGGAAGTAGCCATTGGAACAGAGGTAAAAAAGAAGATAAAGAATAATTCTCTTCGACATGTCGGAACGAAACCCGACCCGGAATCCGACCCGGACAATTCCAGCCCGATTTCGGTCCTCGATGACGGCGGATTCGATTTCTCGGAGAAATGTCAGACCGGCGGCGACCGGAGCGAGGAGGAGGTGGGAAGGAGCTGCAGGTTGGCAGATGAAGATATAAGAGAGGCGAATTGGAGTGTGAAGAATGTGTGGGAATATGAAGCGTTAGAAGAATTATGCATGGAGCTTGAACGACATGTCGTCAATGTATTGTTGGTTCAGACCCTAGATGACCTTGTG

Protein sequence

MPNSGQPNPGCFSGMLRRLFCTGNLPTHPSDALNEPQLEKAAEAKPGPGVVARLMGLSSLPDANWVPNHRAPGAVPRSKSVNFADYLLHFDSSQAHHRRVRTSASFREVPPLNPHNDFLVLYTKDYFDGYEAEIESNLKEPETHRLREAKQGKELQSSNVKKKKKNSRNEFKISKLKNEPRREVGKDFSKKNRKCSSNKDSVSVLPSCKYKQSVTKKLGAANLKSPQKKPPKQKEVAIGTEVKKKIKNNSLRHVGTKPDPESDPDNSSPISVLDDGGFDFSEKCQTGGDRSEEEVGRSCRLADEDIREANWSVKNVWEYEALEELCMELERHVVNVLLVQTLDDLV

Homology

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BLAST of MS016738 vs. NCBI nr
Match: XP_022145653.1 (uncharacterized protein LOC111015050 [Momordica charantia])

HSP 1 Score: 672.2 bits (1733), Expect = 2.5e-189
Identity = 345/358 (96.37%), Postives = 345/358 (96.37%), Query Frame = 0


Query: 1   MPNSGQPNPGCFSGMLRRLFCTGNLPTHPSDALNEPQLEKAAEAKPGPGVVARLMGLSSL 60
           MPNSGQPNPGCFSGMLRRL CTGNLPTHPSDALNEPQLEKAAEAKPGPGVVARLMGLSSL
Sbjct: 1   MPNSGQPNPGCFSGMLRRLLCTGNLPTHPSDALNEPQLEKAAEAKPGPGVVARLMGLSSL 60

Query: 61  PDANWVPNHRAPGAVPRSKSVNFADYLLHFDSSQAHHRRVRTSASFREVPPLNPHNDFLV 120
           PDANWVPNHRAPGAVPRSKSVNFADYLLHFDSSQAHHRRVRTSASFREVPPLNPHNDFLV
Sbjct: 61  PDANWVPNHRAPGAVPRSKSVNFADYLLHFDSSQAHHRRVRTSASFREVPPLNPHNDFLV 120

Query: 121 LYTKDYFDGYEAEIESNLKEPETHRLREAKQGKELQSSNVKKKKKNSRNEFKISKLKNEP 180
           LYTKDYFDGYEAEIESNLKEPETHRLREAKQGKELQSSNVKKKKKNSRNEFKISKLKNEP
Sbjct: 121 LYTKDYFDGYEAEIESNLKEPETHRLREAKQGKELQSSNVKKKKKNSRNEFKISKLKNEP 180

Query: 181 RREVGKDFSKKNRKCSSNKDSVSVLPSCKYKQSVTKKLGAANLKSPQKKPPKQKEVAIGT 240
           RREVGKDFSKKNRKCSSNKDSVSVLPSCKYKQSVTKKLGAANLKSPQKKPPKQKEVAIGT
Sbjct: 181 RREVGKDFSKKNRKCSSNKDSVSVLPSCKYKQSVTKKLGAANLKSPQKKPPKQKEVAIGT 240

Query: 241 EVKKKIKNNSLRHVGTKPDPESDPDNSSPISVLDDGGFDFS------------EKCQTGG 300
           EVKKKIKNNSLRHVGTKPDPESDPDNSSPISVLDDGGFDFS            EKCQTGG
Sbjct: 241 EVKKKIKNNSLRHVGTKPDPESDPDNSSPISVLDDGGFDFSDERSSPKTEGGLEKCQTGG 300

Query: 301 DRSEEEVGRSCRLADEDIREANWSVKNVWEYEALEELCMELERHVVNVLLVQTLDDLV 347
           DRSEEEVGRSCRLADEDIREANWSVKNVWEYEALEELCMELERHVVNVLLVQTLDDLV
Sbjct: 301 DRSEEEVGRSCRLADEDIREANWSVKNVWEYEALEELCMELERHVVNVLLVQTLDDLV 358

[Close]

BLAST of MS016738 vs. NCBI nr
Match: XP_038905307.1 (uncharacterized protein LOC120091377 [Benincasa hispida])

HSP 1 Score: 422.5 bits (1085), Expect = 3.4e-114
Identity = 242/367 (65.94%), Postives = 280/367 (76.29%), Query Frame = 0


Query: 1   MPNSGQPNPGCFSGMLRRLFCTGNLPTHPSDALNEPQLE--------KAAEAKPGPGVVA 60
           MPNSG PNPGCFSGMLRRL CTGNLPTHPS+ALNE Q +         A  A+ GPGVVA
Sbjct: 1   MPNSGDPNPGCFSGMLRRLLCTGNLPTHPSEALNESQFDIPKAEAKLVARAAESGPGVVA 60

Query: 61  RLMGLSSLPDANWVPNHRA-PGAVPRSKSVNFADYLLHFDSSQAHHRRVRTSASFREVPP 120
           RLMGLSSLPDANWVPNHRA PGAV RSKSVNFADYLL FDS+QAHHRR+RTSASFREVPP
Sbjct: 61  RLMGLSSLPDANWVPNHRARPGAVSRSKSVNFADYLLDFDSNQAHHRRIRTSASFREVPP 120

Query: 121 LNPHNDFLVLYTKDYFDGYEAEIESNLKEPETHRLREAKQGKELQSSNV----KKKKKNS 180
           LNPHN F VLY KDYFD Y   I+SNLK+PET RL E KQ  E  S+++    KKKK+N+
Sbjct: 121 LNPHNHFFVLYAKDYFDSY--GIDSNLKKPETQRLEEGKQSNEQSSNDLKKTKKKKKENA 180

Query: 181 RNEFKISKLKNEPRREVGKDFSKKNRKCSSNKDSVSVLPSC--KYKQSVTKKLGAANLKS 240
           RN+ KISKLK+EPRR   K F+ +++KCS  KDSVSVLPSC  KYKQS+ K  G      
Sbjct: 181 RNDIKISKLKDEPRRVNRKKFT-ESKKCSMGKDSVSVLPSCKYKYKQSIPKNDGVVT--- 240

Query: 241 PQKKPPKQKEVAIGTEVKKKIKNNSLRHVGTKPDPESDPDNSSPISVLDDGGFDFSEKCQ 300
            +KKP K K+ AIGTE+ KK KNN +RHVG KPD E DP+NSSP+SVLD G  DFS++ Q
Sbjct: 241 -RKKPTKPKKAAIGTELDKKKKNN-VRHVGQKPDIEPDPENSSPVSVLDVGRIDFSDEMQ 300

Query: 301 TGGDRSEEEVG----RSCRLADEDIREANWS--VKNVWEYEALEELCMELERHVVNVLLV 347
            G +  + + G    R CRLA++DIR+A W+  +KNV E EALEELCME+ERHVV+VLLV
Sbjct: 301 IGVNNRDYDYGELVERICRLAEKDIRDAKWTAKIKNVDESEALEELCMEIERHVVDVLLV 359

[Close]

BLAST of MS016738 vs. NCBI nr
Match: XP_022971608.1 (uncharacterized protein LOC111470283 [Cucurbita maxima])

HSP 1 Score: 388.7 bits (997), Expect = 5.4e-104
Identity = 225/358 (62.85%), Postives = 263/358 (73.46%), Query Frame = 0


Query: 7   PNPGCFSGMLRRLFCTGNLPTHPSDALNEPQLEK---AAEAKPGPGVVARLMGLSSLPDA 66
           PN  CF  +LRRL C+GNLPTHPS+ALNEP  +       ++PGPGVVARLMGLSSLPDA
Sbjct: 2   PNSACFPALLRRLLCSGNLPTHPSEALNEPNSQPKLVPPASEPGPGVVARLMGLSSLPDA 61

Query: 67  NWVPNHRAPGAVPRSKSVNFADYLLHFDSSQAHHRRVRTSASFREVPPLNPHNDFLVLYT 126
           NWVP +RAPGAV RSKSVNFADYLLHFDS+QAHHRR+RTSASFREVPPLNP N+F VLYT
Sbjct: 62  NWVPTNRAPGAVSRSKSVNFADYLLHFDSNQAHHRRIRTSASFREVPPLNPQNEFFVLYT 121

Query: 127 KDYFDGYEAEIESNLKEPETHRLREAKQGKELQSSNVKKKKKNSRNEFKISKLKNEPRRE 186
           KDYFDGY  EIES+LK+ ET R  E KQGKE   SN  K KK   NE KISKLK+EPRRE
Sbjct: 122 KDYFDGYAVEIESSLKKLETQRFGEEKQGKEQSRSNDMKTKKKKNNESKISKLKDEPRRE 181

Query: 187 VGKDFSKKNRKCSSNKDSVSVLPSCKY--KQSVTKKLGAANLKSPQKKPPKQKEVAIGTE 246
           +GK+F+  ++KCS++KDS SVLPS KY  KQ+V K     N     KKP KQ+E AI TE
Sbjct: 182 IGKNFT-NSKKCSTSKDSFSVLPSGKYKDKQNVPKNGHTVN----SKKPVKQQESAIETE 241

Query: 247 VKKKI------KNNSLRHVGTKPDPESDPDNSSPISVLDDGGFDFSEKCQ----TGGD-- 306
           + K I      K N  RHVG K  PESD +NSSP+SVLD  G  FS++ +     GGD  
Sbjct: 242 LNKNIKKKEKKKKNVSRHVGQKSKPESDQENSSPVSVLDVQGIYFSDEMEETDVKGGDAA 301

Query: 307 -RSEEEVGRSCRLADEDIREANWSVKNVWEYEALEELCMELERHVVNVLLVQTLDDLV 347
            RSE+ V R CRLA+EDI+EA W +K V     +EELCME+ERHVVNVLL ++L++LV
Sbjct: 302 GRSEKLVERICRLAEEDIKEATWRIKKV----DVEELCMEVERHVVNVLLDESLEELV 350

[Close]

BLAST of MS016738 vs. NCBI nr
Match: XP_023540166.1 (uncharacterized protein LOC111800619 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 380.6 bits (976), Expect = 1.5e-101
Identity = 221/357 (61.90%), Postives = 263/357 (73.67%), Query Frame = 0


Query: 7   PNPGCFSGMLRRLFCTGNLPTHPSDALNEPQLEK---AAEAKPGPGVVARLMGLSSLPDA 66
           PN  CF  +LRRL C+GNLPTHPS+ALNEP  +       ++P PGVVARLMGLSSLPDA
Sbjct: 2   PNSACFPALLRRLLCSGNLPTHPSEALNEPNSQPKLVPPASEPSPGVVARLMGLSSLPDA 61

Query: 67  NWVPNHRAPGAVPRSKSVNFADYLLHFDSSQAHHRRVRTSASFREVPPLNPHNDFLVLYT 126
           NWVP +RAPGAV RSKSVNFADYLLHFD++QAHHRR+RTSASFREVP L+P N+F VLYT
Sbjct: 62  NWVPTNRAPGAVSRSKSVNFADYLLHFDTNQAHHRRIRTSASFREVPALSPQNEFFVLYT 121

Query: 127 KDYFDGYEAEIESNLKEPETHRLREAKQGKELQSSN-VKKKKKNSRNEFKISKLKNEPRR 186
           KDYFDGY  EIESNLK+ ET R  E KQGKE   SN +K KKK  +NE KISKLK+EPRR
Sbjct: 122 KDYFDGYAVEIESNLKKLETQRFGEEKQGKEQSRSNDMKTKKKKKKNESKISKLKDEPRR 181

Query: 187 EVGKDFSKKNRKCSSNKDSVSVLPSCKY--KQSVTKKLGAANLKSPQKKPPKQKEVAIGT 246
           E+GK+F+  ++KCS++KDS SVLPS KY  KQ+V K     N     KKP +Q+E AI T
Sbjct: 182 EIGKNFT-NSKKCSTSKDSFSVLPSGKYRDKQNVPKNGHTVN----SKKPVRQQESAIET 241

Query: 247 E----VKKKIKNNSLRHVGTKPDPESDPDNSSPISVLDDGGFDFSEKCQ----TGGD--- 306
           E    + KK K N  RHVG    PESD +NSSP+SVLD  G  FS++ +     GGD   
Sbjct: 242 ELNKNINKKKKKNVSRHVGQTSKPESDQENSSPVSVLDVQGIYFSDEMEETDVKGGDAAG 301

Query: 307 RSEEEVGRSCRLADEDIREANWSVKNVWEYEALEELCMELERHVVNVLLVQTLDDLV 347
           RSE+ V R CRLA+EDI+EA W +K V     +EELCME+ERHVVN LLV++L++LV
Sbjct: 302 RSEKLVERICRLAEEDIKEATWRIKKV----DVEELCMEVERHVVNALLVESLEELV 349

[Close]

BLAST of MS016738 vs. NCBI nr
Match: KAG6596341.1 (hypothetical protein SDJN03_09521, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 380.6 bits (976), Expect = 1.5e-101
Identity = 222/358 (62.01%), Postives = 260/358 (72.63%), Query Frame = 0


Query: 7   PNPGCFSGMLRRLFCTGNLPTHPSDALNEPQLEK---AAEAKPGPGVVARLMGLSSLPDA 66
           PN  CF  +LRRL C+GNLPTHPS+ALNEP  E       ++P PGVVARLMGLSSLPDA
Sbjct: 2   PNSACFPALLRRLLCSGNLPTHPSEALNEPNSEPKLVPPASEPSPGVVARLMGLSSLPDA 61

Query: 67  NWVPNHRAPGAVPRSKSVNFADYLLHFDSSQAHHRRVRTSASFREVPPLNPHNDFLVLYT 126
           NWVP +RAPGAV RSKSVNFADYLLHFD++QAHHRR+RTSASFREVP L+P N+F VLYT
Sbjct: 62  NWVPANRAPGAVSRSKSVNFADYLLHFDTNQAHHRRIRTSASFREVPALSPQNEFFVLYT 121

Query: 127 KDYFDGYEAEIESNLKEPETHRLREAKQGKELQSSNVKKKKKNSRNEFKISKLKNEPRRE 186
           KDYFDGY  EIESNLK+ ET R  E KQGKE   SN  K KK   NE KISKLK+EPRRE
Sbjct: 122 KDYFDGYAVEIESNLKKLETQRFGEEKQGKEQSRSNDMKTKKKKMNESKISKLKDEPRRE 181

Query: 187 VGKDFSKKNRKCSSNKDSVSVLPSCKY--KQSVTKKLGAANLKSPQKKPPKQKEVAIGTE 246
           +GK+F+  ++KCS++KDS SVLP  KY  KQ+V K     N     KKP KQ+E AI TE
Sbjct: 182 IGKNFT-NSKKCSTSKDSFSVLPPGKYRDKQNVPKNGHTVN----SKKPVKQQESAIETE 241

Query: 247 V------KKKIKNNSLRHVGTKPDPESDPDNSSPISVLDDGGFDFSEKCQ----TGGD-- 306
           +      KKK K N  RHVG    PESD +NSSP+SVLD  G  FS++ +     GGD  
Sbjct: 242 LNKNIKKKKKKKKNVSRHVGQTSQPESDQENSSPVSVLDVQGIYFSDEMEETDVKGGDAA 301

Query: 307 -RSEEEVGRSCRLADEDIREANWSVKNVWEYEALEELCMELERHVVNVLLVQTLDDLV 347
            RSE+ V R CRLA+EDI+EA W +K V     +EELCME+ERHVVN LLV++L++LV
Sbjct: 302 GRSEKLVERICRLAEEDIKEATWRIKKV----DVEELCMEVERHVVNALLVESLEELV 350

[Close]

BLAST of MS016738 vs. ExPASy TrEMBL
Match: A0A6J1CWJ6 (uncharacterized protein LOC111015050 OS=Momordica charantia OX=3673 GN=LOC111015050 PE=4 SV=1)

HSP 1 Score: 672.2 bits (1733), Expect = 1.2e-189
Identity = 345/358 (96.37%), Postives = 345/358 (96.37%), Query Frame = 0


Query: 1   MPNSGQPNPGCFSGMLRRLFCTGNLPTHPSDALNEPQLEKAAEAKPGPGVVARLMGLSSL 60
           MPNSGQPNPGCFSGMLRRL CTGNLPTHPSDALNEPQLEKAAEAKPGPGVVARLMGLSSL
Sbjct: 1   MPNSGQPNPGCFSGMLRRLLCTGNLPTHPSDALNEPQLEKAAEAKPGPGVVARLMGLSSL 60

Query: 61  PDANWVPNHRAPGAVPRSKSVNFADYLLHFDSSQAHHRRVRTSASFREVPPLNPHNDFLV 120
           PDANWVPNHRAPGAVPRSKSVNFADYLLHFDSSQAHHRRVRTSASFREVPPLNPHNDFLV
Sbjct: 61  PDANWVPNHRAPGAVPRSKSVNFADYLLHFDSSQAHHRRVRTSASFREVPPLNPHNDFLV 120

Query: 121 LYTKDYFDGYEAEIESNLKEPETHRLREAKQGKELQSSNVKKKKKNSRNEFKISKLKNEP 180
           LYTKDYFDGYEAEIESNLKEPETHRLREAKQGKELQSSNVKKKKKNSRNEFKISKLKNEP
Sbjct: 121 LYTKDYFDGYEAEIESNLKEPETHRLREAKQGKELQSSNVKKKKKNSRNEFKISKLKNEP 180

Query: 181 RREVGKDFSKKNRKCSSNKDSVSVLPSCKYKQSVTKKLGAANLKSPQKKPPKQKEVAIGT 240
           RREVGKDFSKKNRKCSSNKDSVSVLPSCKYKQSVTKKLGAANLKSPQKKPPKQKEVAIGT
Sbjct: 181 RREVGKDFSKKNRKCSSNKDSVSVLPSCKYKQSVTKKLGAANLKSPQKKPPKQKEVAIGT 240

Query: 241 EVKKKIKNNSLRHVGTKPDPESDPDNSSPISVLDDGGFDFS------------EKCQTGG 300
           EVKKKIKNNSLRHVGTKPDPESDPDNSSPISVLDDGGFDFS            EKCQTGG
Sbjct: 241 EVKKKIKNNSLRHVGTKPDPESDPDNSSPISVLDDGGFDFSDERSSPKTEGGLEKCQTGG 300

Query: 301 DRSEEEVGRSCRLADEDIREANWSVKNVWEYEALEELCMELERHVVNVLLVQTLDDLV 347
           DRSEEEVGRSCRLADEDIREANWSVKNVWEYEALEELCMELERHVVNVLLVQTLDDLV
Sbjct: 301 DRSEEEVGRSCRLADEDIREANWSVKNVWEYEALEELCMELERHVVNVLLVQTLDDLV 358

[Close]

BLAST of MS016738 vs. ExPASy TrEMBL
Match: A0A6J1I7D0 (uncharacterized protein LOC111470283 OS=Cucurbita maxima OX=3661 GN=LOC111470283 PE=4 SV=1)

HSP 1 Score: 388.7 bits (997), Expect = 2.6e-104
Identity = 225/358 (62.85%), Postives = 263/358 (73.46%), Query Frame = 0


Query: 7   PNPGCFSGMLRRLFCTGNLPTHPSDALNEPQLEK---AAEAKPGPGVVARLMGLSSLPDA 66
           PN  CF  +LRRL C+GNLPTHPS+ALNEP  +       ++PGPGVVARLMGLSSLPDA
Sbjct: 2   PNSACFPALLRRLLCSGNLPTHPSEALNEPNSQPKLVPPASEPGPGVVARLMGLSSLPDA 61

Query: 67  NWVPNHRAPGAVPRSKSVNFADYLLHFDSSQAHHRRVRTSASFREVPPLNPHNDFLVLYT 126
           NWVP +RAPGAV RSKSVNFADYLLHFDS+QAHHRR+RTSASFREVPPLNP N+F VLYT
Sbjct: 62  NWVPTNRAPGAVSRSKSVNFADYLLHFDSNQAHHRRIRTSASFREVPPLNPQNEFFVLYT 121

Query: 127 KDYFDGYEAEIESNLKEPETHRLREAKQGKELQSSNVKKKKKNSRNEFKISKLKNEPRRE 186
           KDYFDGY  EIES+LK+ ET R  E KQGKE   SN  K KK   NE KISKLK+EPRRE
Sbjct: 122 KDYFDGYAVEIESSLKKLETQRFGEEKQGKEQSRSNDMKTKKKKNNESKISKLKDEPRRE 181

Query: 187 VGKDFSKKNRKCSSNKDSVSVLPSCKY--KQSVTKKLGAANLKSPQKKPPKQKEVAIGTE 246
           +GK+F+  ++KCS++KDS SVLPS KY  KQ+V K     N     KKP KQ+E AI TE
Sbjct: 182 IGKNFT-NSKKCSTSKDSFSVLPSGKYKDKQNVPKNGHTVN----SKKPVKQQESAIETE 241

Query: 247 VKKKI------KNNSLRHVGTKPDPESDPDNSSPISVLDDGGFDFSEKCQ----TGGD-- 306
           + K I      K N  RHVG K  PESD +NSSP+SVLD  G  FS++ +     GGD  
Sbjct: 242 LNKNIKKKEKKKKNVSRHVGQKSKPESDQENSSPVSVLDVQGIYFSDEMEETDVKGGDAA 301

Query: 307 -RSEEEVGRSCRLADEDIREANWSVKNVWEYEALEELCMELERHVVNVLLVQTLDDLV 347
            RSE+ V R CRLA+EDI+EA W +K V     +EELCME+ERHVVNVLL ++L++LV
Sbjct: 302 GRSEKLVERICRLAEEDIKEATWRIKKV----DVEELCMEVERHVVNVLLDESLEELV 350

[Close]

BLAST of MS016738 vs. ExPASy TrEMBL
Match: A0A6J1FV14 (uncharacterized protein LOC111447527 OS=Cucurbita moschata OX=3662 GN=LOC111447527 PE=4 SV=1)

HSP 1 Score: 379.4 bits (973), Expect = 1.6e-101
Identity = 221/358 (61.73%), Postives = 259/358 (72.35%), Query Frame = 0


Query: 7   PNPGCFSGMLRRLFCTGNLPTHPSDALNEPQLEK---AAEAKPGPGVVARLMGLSSLPDA 66
           PN  CF  + RRL C+GNLPTHPS+ALNEP  E       ++P PGVVARLMGLSSLPDA
Sbjct: 2   PNSACFPALFRRLLCSGNLPTHPSEALNEPNSEPKLVPPASEPSPGVVARLMGLSSLPDA 61

Query: 67  NWVPNHRAPGAVPRSKSVNFADYLLHFDSSQAHHRRVRTSASFREVPPLNPHNDFLVLYT 126
           NWVP +RAPGAV RSKSVNFADYLLHFD++QAHHRR+RTSASFREVP L+P N+F VLYT
Sbjct: 62  NWVPANRAPGAVSRSKSVNFADYLLHFDTNQAHHRRIRTSASFREVPALSPQNEFFVLYT 121

Query: 127 KDYFDGYEAEIESNLKEPETHRLREAKQGKELQSSNVKKKKKNSRNEFKISKLKNEPRRE 186
           KDYFDGY  EIESNLK+ ET R  E KQGKE   SN  K KK   NE KISKLK+EPRRE
Sbjct: 122 KDYFDGYAVEIESNLKKLETQRFGEEKQGKEQSRSNNMKTKKKKMNESKISKLKDEPRRE 181

Query: 187 VGKDFSKKNRKCSSNKDSVSVLPSCKY--KQSVTKKLGAANLKSPQKKPPKQKEVAIGTE 246
           +GK+F+  ++KCS++KDS SVLP  KY  KQ+V K     N     KKP KQ+E AI TE
Sbjct: 182 IGKNFT-NSKKCSTSKDSFSVLPPGKYRDKQNVPKNGHTVN----SKKPVKQQESAIETE 241

Query: 247 V------KKKIKNNSLRHVGTKPDPESDPDNSSPISVLDDGGFDFSEKCQ----TGGD-- 306
           +      KKK K N  RHVG    PESD +NSSP+SVLD  G  FS++ +     GGD  
Sbjct: 242 LNKNIKKKKKKKKNVSRHVGQTSQPESDQENSSPVSVLDVQGIYFSDEMEETDVKGGDAA 301

Query: 307 -RSEEEVGRSCRLADEDIREANWSVKNVWEYEALEELCMELERHVVNVLLVQTLDDLV 347
            RSE+ + R CRLA+EDI+EA W +K V     +EELCME+ERHVVN LLV++LD+LV
Sbjct: 302 GRSEKLLERICRLAEEDIKEATWRIKKV----DVEELCMEVERHVVNALLVESLDELV 350

[Close]

BLAST of MS016738 vs. ExPASy TrEMBL
Match: A0A0A0L9V4 (VARLMGL domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G731120 PE=4 SV=1)

HSP 1 Score: 377.1 bits (967), Expect = 7.9e-101
Identity = 224/361 (62.05%), Postives = 261/361 (72.30%), Query Frame = 0


Query: 1   MPNSGQPNPGCFSGMLRRLFCTGNLPTHPSDALNEPQLE--------KAAEAKPGPGVVA 60
           MPNS  PN  CFSG+LRRL CTGNLPTHPS+ALN+ Q +         A  A+  PGVVA
Sbjct: 1   MPNSAHPNSCCFSGILRRLLCTGNLPTHPSEALNDSQFDIPKTEAKLVAQSAESTPGVVA 60

Query: 61  RLMGLSSLPDANWVPNHRA-PGAVPRSKSVNFADYLLHFDSSQAHHRRVRTSASFREVPP 120
           RLMGLSSLPDANWVPNHRA PGAV RSKSVNFADYLL FDS+Q+HHRR+RTSASFREV  
Sbjct: 61  RLMGLSSLPDANWVPNHRARPGAVSRSKSVNFADYLLDFDSNQSHHRRIRTSASFREV-- 120

Query: 121 LNPHNDFLVLYTKDYFDGYEAEIESNLKEPETHRLREAKQGKELQSSNVKKKKKNSRNEF 180
             PHND+ VLYTKDYFDGY   IESN K+PET R  E KQ     +   KKKK+N RNE 
Sbjct: 121 --PHNDYFVLYTKDYFDGY--GIESNSKKPETQRFDEGKQSSNDLNKKKKKKKENGRNEM 180

Query: 181 KISKLKNEPRREVGKDFSKKNRKCSSNKDSVSVLPSCKY--KQSVTKKLGAANLKSPQKK 240
           KISKLK+EPRR   K+F+ +++KCS  KDS SVLPSCK+  KQ+V +   +      QKK
Sbjct: 181 KISKLKDEPRRVNRKNFT-ESKKCSMGKDSFSVLPSCKHKCKQNVPRNESSV----IQKK 240

Query: 241 PPKQKEVAIGTEVKKKIKNNSLRHVGTKPDPESDPDNSSPISVLDDGGFDFSEKCQTGGD 300
           P KQKE AI TE+ KK K N +RHV  KPD E D +NSSP+SVLD G  DFS++ Q GG 
Sbjct: 241 PTKQKEAAIRTELNKKKKKN-VRHVERKPDVEPDSENSSPVSVLDVGRIDFSDERQIGGK 300

Query: 301 RSEEEVG----RSCRLADEDIREANWS--VKNVWEYEALEELCMELERHVVNVLLVQTLD 345
               + G    R CRLA+EDIREA W+  +KNV E EALEE+CME+ERHVV+ LLV TL+
Sbjct: 301 NRVYDYGELVERICRLAEEDIREAKWTAEIKNVDESEALEEICMEIERHVVDALLVHTLN 349

[Close]

BLAST of MS016738 vs. ExPASy TrEMBL
Match: E5GBE7 (VARLMGL domain-containing protein OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1)

HSP 1 Score: 370.9 bits (951), Expect = 5.7e-99
Identity = 220/361 (60.94%), Postives = 261/361 (72.30%), Query Frame = 0


Query: 1   MPNSGQPNPGCFSGMLRRLFCTGNLPTHPSDALNEPQLE--------KAAEAKPGPGVVA 60
           MPNS  PN GCFSG+LRRL CTGNLPTHPS+AL+E Q +         A  A+  PGVVA
Sbjct: 1   MPNSAHPNSGCFSGILRRLLCTGNLPTHPSEALHESQFDIPKTEAKLAAQSAESTPGVVA 60

Query: 61  RLMGLSSLPDANWVPNHRA-PGAVPRSKSVNFADYLLHFDSSQAHHRRVRTSASFREVPP 120
           RLMGLSSLPDANWVPNH+  PGAV RSKSVNFADYLL FDS+Q+HHRR+RTSASFREVPP
Sbjct: 61  RLMGLSSLPDANWVPNHQVRPGAVSRSKSVNFADYLLDFDSNQSHHRRIRTSASFREVPP 120

Query: 121 LNPHNDFLVLYTKDYFDGYEAEIESNLKEPETHRLREAKQGKELQSSNVKKKKKNSRNEF 180
           LNPHNDF VLYTKD F+GY   IESNLK+PET R  E KQ         KKKK+N+RNE 
Sbjct: 121 LNPHNDFFVLYTKDCFNGY--GIESNLKKPETQRFDEGKQSSN-DLKKKKKKKENARNEM 180

Query: 181 KISKLKNEPRREVGKDFSKKNRKCSSNKDSVSVLPSCKY--KQSVTKKLGAANLKSPQKK 240
           KISKLK+EPRR   K+F+ +++KCS  KDS SVLPSCK+  K+S+ +   A      QKK
Sbjct: 181 KISKLKDEPRRVSRKNFT-ESKKCSMGKDSFSVLPSCKHKCKRSIPRNESAV----IQKK 240

Query: 241 PPKQKEVAIGTEVKKKIKNNSLRHVGTKPDPESDPDNSSPISVLDDGGFDFSEKCQTGGD 300
           P  QKE  I TE+ KK K N +RHV  KPD + D +NSSP+SVLD G  +FS++ Q GG 
Sbjct: 241 PTMQKEATIRTELNKKKKKN-VRHVERKPDLQLDSENSSPVSVLDVGRIEFSDERQIGGK 300

Query: 301 RSEEEVG----RSCRLADEDIREANWS--VKNVWEYEALEELCMELERHVVNVLLVQTLD 345
               + G    R CRLA+EDI EA W+  +KN  + EALEE+CME+ERHVV+ LLV TL+
Sbjct: 301 NRVYDYGELVERICRLAEEDI-EAKWTAEIKNADKSEALEEICMEIERHVVDALLVHTLN 351

[Close]

BLAST of MS016738 vs. TAIR 10
Match: AT5G01370.1 (ALC-interacting protein 1 )

HSP 1 Score: 71.2 bits (173), Expect = 1.8e-12
Identity = 114/462 (24.68%), Postives = 180/462 (38.96%), Query Frame = 0


Query: 1   MPNSGQPNPGCFSGMLRRLFCTGNLPTHPSDAL----------------NEPQLEKAAEA 60
           M     P  GC + ++RRL C+G+  THPSD +                 EP+ E   E 
Sbjct: 1   MTQRANPKTGCLTAVVRRLLCSGSQQTHPSDNILDSDETLQLLISYEENEEPKKEIKTET 60

Query: 61  K--------------PGPGVVARLMGLSSLPDANWVPNHRAPG------------AVPRS 120
           +              P P VVA+LMGL          +H APG            AV RS
Sbjct: 61  ETQNDDVSLHPPPPPPPPNVVAKLMGL----------DHPAPGSKRFRYFDDSGSAVTRS 120

Query: 121 KSVNFADYLL--HFD--------SSQAHHRRVRTSASFREVPPLNPH---------NDFL 180
           KSVNF DY+L  H +          Q   RRV+ S SFRE+ P +           +DFL
Sbjct: 121 KSVNFMDYILKGHEEEEENEDEKDGQRQCRRVKASVSFREIVPTSARWSSNNQQKKHDFL 180

Query: 181 VLYTKDYFDGYEAEIESNL-KEPETHRLREAKQGKELQSSNVKKKKKNSRNEFKISKLKN 240
           +LY     +  E    S+  +     ++ E  +   L  S  KKK+    NE    K K+
Sbjct: 181 LLYLDKLDEKRELVGSSSFSRSKRFEKVVEDSKKPPLPPSAPKKKE----NEKVAKKFKD 240

Query: 241 EPRREVGKDFSKKNRKCSSNKDSVSVLPSCKYKQSVTKKLGAANLKSPQKKPPKQKEVAI 300
           EPR+   K       K S N++ V+     ++              SP K     ++ A+
Sbjct: 241 EPRKVAKK-------KKSENRNDVNGAKKVRW------------FLSPSKSKSSSEKTAL 300

Query: 301 GTEVKKKIKNNSLRHVGTKPDPESDPDNSSPISVLDDGGFDF----SEKCQTGGD----- 346
             E  K I+ +    +G + +   +  N+SP+SVLD   +D+     +   +GGD     
Sbjct: 301 LGE-SKNIRADEF--IGKESESPENKSNASPVSVLDRDLYDYLILDDDYYFSGGDSESAS 360

[Close]

BLAST of MS016738 vs. TAIR 10
Match: AT5G58630.1 (unknown protein; Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 52.0 bits (123), Expect = 1.1e-06
Identity = 76/277 (27.44%), Postives = 116/277 (41.88%), Query Frame = 0


Query: 10  GCFSGMLRRLFCTGNLPTHPSDALNEPQL-------EKAAEAKPGPGVVARLMGLSSLPD 69
           GC S +L+R  C+G   T+PSD + EP         E       GPG VARLMGL S+P 
Sbjct: 23  GCISIILQRFLCSGTSSTYPSDLITEPSFKSRDFIPEPLRGRGDGPGAVARLMGLDSIPV 82

Query: 70  ANWVPNHRAPGAVPRSKSVNFADYLLHFDSSQAHHRRVRTSASFREVPPLNPHNDFLVLY 129
            +          + RS SVN        D  Q  HRRV+++  + E+      +DF +L 
Sbjct: 83  ID------KTRFLSRSHSVNHLK--RDDDEIQGKHRRVKSTLEYVEL----EDDDFFIL- 142

Query: 130 TKDYFDGYEAEIESNLKEPETHRLREAKQGKELQSSNVKKK----KKNSRNEFKISKLKN 189
                     E + N KE  T   ++ +  +E QS    +     K+N+ N   +S    
Sbjct: 143 --------SFEKDKNDKELGTRNCKKRRDTRENQSKTEDQTHLEGKENNINAMNVS--PG 202

Query: 190 EPRREVGKDFSKKNRKCSSNKDSVSVLPSCKYKQSVTKKLGAANLKSPQKKPPKQKEVAI 249
             R E+G+   ++  K  S +         K +Q++ K+    +     K     +E  +
Sbjct: 203 RERLEIGELMQRRKEKRKSRRKR----KETKLRQNIEKENWVQSNGCWSK-----EEDLV 256

Query: 250 GTEVKKKIKNNSLRHVGTKPDPE-SDPDNSSPISVLD 275
             EV+            TK + E    D+SSP+SVLD
Sbjct: 263 NCEVR-----------STKEEEECGSCDDSSPVSVLD 256

The following BLAST results are available for this feature:

BLAST of MS016738 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022145653.1	2.5e-189	96.37	uncharacterized protein LOC111015050 [Momordica charantia]	[more]
XP_038905307.1	3.4e-114	65.94	uncharacterized protein LOC120091377 [Benincasa hispida]	[more]
XP_022971608.1	5.4e-104	62.85	uncharacterized protein LOC111470283 [Cucurbita maxima]	[more]
XP_023540166.1	1.5e-101	61.90	uncharacterized protein LOC111800619 [Cucurbita pepo subsp. pepo]	[more]
KAG6596341.1	1.5e-101	62.01	hypothetical protein SDJN03_09521, partial [Cucurbita argyrosperma subsp. sorori...	[more]

BLAST of MS016738 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of MS016738 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CWJ6	1.2e-189	96.37	uncharacterized protein LOC111015050 OS=Momordica charantia OX=3673 GN=LOC111015...	[more]
A0A6J1I7D0	2.6e-104	62.85	uncharacterized protein LOC111470283 OS=Cucurbita maxima OX=3661 GN=LOC111470283...	[more]
A0A6J1FV14	1.6e-101	61.73	uncharacterized protein LOC111447527 OS=Cucurbita moschata OX=3662 GN=LOC1114475...	[more]
A0A0A0L9V4	7.9e-101	62.05	VARLMGL domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G731120 PE=...	[more]
E5GBE7	5.7e-99	60.94	VARLMGL domain-containing protein OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=...	[more]

BLAST of MS016738 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G01370.1	1.8e-12	24.68	ALC-interacting protein 1	[more]
AT5G58630.1	1.1e-06	27.44	unknown protein; Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0...	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR032795	DUF3741-associated sequence motif	PFAM	PF14383	VARLMGL	coord: 48..64 e-value: 5.7E-8 score: 32.1
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 219..277
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 171..197
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 228..260
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 138..202
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 138..164
None	No IPR available	PANTHER	PTHR35499	OS05G0128300 PROTEIN	coord: 1..292 coord: 293..346
None	No IPR available	PANTHER	PTHR35499:SF4	ALC-INTERACTING PROTEIN 1	coord: 1..292
None	No IPR available	PANTHER	PTHR35499:SF4	ALC-INTERACTING PROTEIN 1	coord: 293..346

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS016738.1	MS016738.1	mRNA