MS016726 (gene) Bitter gourd (TR) v1

Overview


Name	MS016726
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	RING-type domain-containing protein
Location	scaffold563: 357147 .. 358193 (-)
RNA-Seq Expression	MS016726
Synteny	MS016726

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

GCTTCTGATTATCCCAGGGAATATGATGGTGCATGCCTTCAGATGAGATTATCCTACAGCCCAGCTGCACAGTTCTTTCTCTTTTTCGTGCAATGGACTGATTGTCATCTTGCTGGTGCATTGGGTTTGCTCAGAATTCTAATATATAAGGTACTGCAGTCTGCATTACAAATGTTGCACTAGTGCCTTCTCCAGTCTGAGTGTGGTGATTGAAATAACTTCAAATTGACCACTAAGGATAAAAACCTGATGAAGTAGCTGATACTGCTTGGATTTACATTTATCTTGTCAAGCCTGTGAATGTACTGAATCTGTTCCAGGCATATGAAGACGGGAAGACTACCATGTCTATTCAAGAAAGAAAGGCTAGTCTCAAAGAGTTCTATGGTAAGTGGACTTGAAGACAAAAGCTGCCATTTCTTATACTTGAGGCGTATAAAAATTTGGAGTCTTGCGAAACCTATTTTTTAGTTTTGCAAATACAGGGGTGATCTTTCCATCTTTATTGCAACTTCAAGGAGGAATTAATGACATAGAAGACAGAAAACAGAGAGAAGTTTATGCTGCCAAATACAAAAGAAAAGATCAATTGGATAGAGGAAAGATCACTGAAATCGACTTGGAAAGAGAGGAGGAATGTGGAATTTGCATGGAGCTAAACTGCATGGTTGTATTGCCTAGTTGCAATCATTCAATGTGCATGAAGTGTTATAGAAGTTGGTAAGCTTTCTGCTGTTTTCATGTTGCCATCAAATACCAAATGACGGAGACTTATTTCTTTGCAAAAAGTGACTGAATTCTTTGGAGCTGATGTTTGCAACTTGTTATCTTGGTTTCAGGCGCACTCGGTCTCAATCATGTCCCTTCTGCCGTGACAGTCTCAAGAGAGTCAACTCTGGAGATCTTTGGATCTATACCGGTAGCAGTGAAATCGTCGACTTGTCCTTCATCTCTAGGGAAAATTTAAAGAGGCTTTTCATGTTCATTGATAAGTTGCCCCTCATTGTTCCAGATCCAAAACTCATCTCTTATTATCTGAACCACTAT

mRNA sequence

GCTTCTGATTATCCCAGGGAATATGATGGTGCATGCCTTCAGATGAGATTATCCTACAGCCCAGCTGCACAGTTCTTTCTCTTTTTCGTGCAATGGACTGATTGTCATCTTGCTGGTGCATTGGGTTTGCTCAGAATTCTAATATATAAGGCATATGAAGACGGGAAGACTACCATGTCTATTCAAGAAAGAAAGGCTAGTCTCAAAGAGTTCTATGGGGTGATCTTTCCATCTTTATTGCAACTTCAAGGAGGAATTAATGACATAGAAGACAGAAAACAGAGAGAAGTTTATGCTGCCAAATACAAAAGAAAAGATCAATTGGATAGAGGAAAGATCACTGAAATCGACTTGGAAAGAGAGGAGGAATGTGGAATTTGCATGGAGCTAAACTGCATGGTTGTATTGCCTAGTTGCAATCATTCAATGTGCATGAAGTGTTATAGAAGTTGGCGCACTCGGTCTCAATCATGTCCCTTCTGCCGTGACAGTCTCAAGAGAGTCAACTCTGGAGATCTTTGGATCTATACCGGTAGCAGTGAAATCGTCGACTTGTCCTTCATCTCTAGGGAAAATTTAAAGAGGCTTTTCATGTTCATTGATAAGTTGCCCCTCATTGTTCCAGATCCAAAACTCATCTCTTATTATCTGAACCACTAT

Coding sequence (CDS)

GCTTCTGATTATCCCAGGGAATATGATGGTGCATGCCTTCAGATGAGATTATCCTACAGCCCAGCTGCACAGTTCTTTCTCTTTTTCGTGCAATGGACTGATTGTCATCTTGCTGGTGCATTGGGTTTGCTCAGAATTCTAATATATAAGGCATATGAAGACGGGAAGACTACCATGTCTATTCAAGAAAGAAAGGCTAGTCTCAAAGAGTTCTATGGGGTGATCTTTCCATCTTTATTGCAACTTCAAGGAGGAATTAATGACATAGAAGACAGAAAACAGAGAGAAGTTTATGCTGCCAAATACAAAAGAAAAGATCAATTGGATAGAGGAAAGATCACTGAAATCGACTTGGAAAGAGAGGAGGAATGTGGAATTTGCATGGAGCTAAACTGCATGGTTGTATTGCCTAGTTGCAATCATTCAATGTGCATGAAGTGTTATAGAAGTTGGCGCACTCGGTCTCAATCATGTCCCTTCTGCCGTGACAGTCTCAAGAGAGTCAACTCTGGAGATCTTTGGATCTATACCGGTAGCAGTGAAATCGTCGACTTGTCCTTCATCTCTAGGGAAAATTTAAAGAGGCTTTTCATGTTCATTGATAAGTTGCCCCTCATTGTTCCAGATCCAAAACTCATCTCTTATTATCTGAACCACTAT

Protein sequence

ASDYPREYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDGKTTMSIQERKASLKEFYGVIFPSLLQLQGGINDIEDRKQREVYAAKYKRKDQLDRGKITEIDLEREEECGICMELNCMVVLPSCNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWIYTGSSEIVDLSFISRENLKRLFMFIDKLPLIVPDPKLISYYLNHY

Homology

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BLAST of MS016726 vs. NCBI nr
Match: XP_022145744.1 (uncharacterized protein LOC111015127 isoform X1 [Momordica charantia] >XP_022145745.1 uncharacterized protein LOC111015127 isoform X1 [Momordica charantia] >XP_022145746.1 uncharacterized protein LOC111015127 isoform X1 [Momordica charantia])

HSP 1 Score: 455.7 bits (1171), Expect = 2.3e-124
Identity = 220/220 (100.00%), Postives = 220/220 (100.00%), Query Frame = 0


Query: 1   ASDYPREYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDGKTTMS 60
           ASDYPREYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDGKTTMS
Sbjct: 23  ASDYPREYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDGKTTMS 82

Query: 61  IQERKASLKEFYGVIFPSLLQLQGGINDIEDRKQREVYAAKYKRKDQLDRGKITEIDLER 120
           IQERKASLKEFYGVIFPSLLQLQGGINDIEDRKQREVYAAKYKRKDQLDRGKITEIDLER
Sbjct: 83  IQERKASLKEFYGVIFPSLLQLQGGINDIEDRKQREVYAAKYKRKDQLDRGKITEIDLER 142

Query: 121 EEECGICMELNCMVVLPSCNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWIYTGSS 180
           EEECGICMELNCMVVLPSCNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWIYTGSS
Sbjct: 143 EEECGICMELNCMVVLPSCNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWIYTGSS 202

Query: 181 EIVDLSFISRENLKRLFMFIDKLPLIVPDPKLISYYLNHY 221
           EIVDLSFISRENLKRLFMFIDKLPLIVPDPKLISYYLNHY
Sbjct: 203 EIVDLSFISRENLKRLFMFIDKLPLIVPDPKLISYYLNHY 242

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BLAST of MS016726 vs. NCBI nr
Match: XP_022145747.1 (probable E3 ubiquitin-protein ligase makorin-3 isoform X2 [Momordica charantia])

HSP 1 Score: 424.5 bits (1090), Expect = 5.7e-115
Identity = 206/206 (100.00%), Postives = 206/206 (100.00%), Query Frame = 0


Query: 15  MRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDGKTTMSIQERKASLKEFYGV 74
           MRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDGKTTMSIQERKASLKEFYGV
Sbjct: 1   MRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDGKTTMSIQERKASLKEFYGV 60

Query: 75  IFPSLLQLQGGINDIEDRKQREVYAAKYKRKDQLDRGKITEIDLEREEECGICMELNCMV 134
           IFPSLLQLQGGINDIEDRKQREVYAAKYKRKDQLDRGKITEIDLEREEECGICMELNCMV
Sbjct: 61  IFPSLLQLQGGINDIEDRKQREVYAAKYKRKDQLDRGKITEIDLEREEECGICMELNCMV 120

Query: 135 VLPSCNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWIYTGSSEIVDLSFISRENLK 194
           VLPSCNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWIYTGSSEIVDLSFISRENLK
Sbjct: 121 VLPSCNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWIYTGSSEIVDLSFISRENLK 180

Query: 195 RLFMFIDKLPLIVPDPKLISYYLNHY 221
           RLFMFIDKLPLIVPDPKLISYYLNHY
Sbjct: 181 RLFMFIDKLPLIVPDPKLISYYLNHY 206

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BLAST of MS016726 vs. NCBI nr
Match: XP_038904042.1 (E3 ubiquitin-protein ligase AIRP2 isoform X1 [Benincasa hispida])

HSP 1 Score: 422.5 bits (1085), Expect = 2.2e-114
Identity = 204/220 (92.73%), Postives = 211/220 (95.91%), Query Frame = 0


Query: 1   ASDYPREYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDGKTTMS 60
           ASDYP+EY GACLQMRLSYSPAAQFFLFFVQWTDCH AGALGLLRILIYKAYEDGKTTMS
Sbjct: 23  ASDYPKEYGGACLQMRLSYSPAAQFFLFFVQWTDCHFAGALGLLRILIYKAYEDGKTTMS 82

Query: 61  IQERKASLKEFYGVIFPSLLQLQGGINDIEDRKQREVYAAKYKRKDQLDRGKITEIDLER 120
           IQERKASLKEFYGVIFPSL+QLQ GI DIE+RKQREVYAAK+KRKD+LD+GKI+EIDLER
Sbjct: 83  IQERKASLKEFYGVIFPSLVQLQRGITDIEERKQREVYAAKFKRKDRLDKGKISEIDLER 142

Query: 121 EEECGICMELNCMVVLPSCNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWIYTGSS 180
           EEECGICMELN  VVLPSCNHSMCMKCYRSWR RSQSCPFCRDSLKRVNSGDLWI T SS
Sbjct: 143 EEECGICMELNSKVVLPSCNHSMCMKCYRSWRARSQSCPFCRDSLKRVNSGDLWICTSSS 202

Query: 181 EIVDLSFISRENLKRLFMFIDKLPLIVPDPKLISYYLNHY 221
           EIVDLSFISRENLKRLFMFIDKLPLIVPDPKLISYYLNHY
Sbjct: 203 EIVDLSFISRENLKRLFMFIDKLPLIVPDPKLISYYLNHY 242

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BLAST of MS016726 vs. NCBI nr
Match: XP_008442427.1 (PREDICTED: uncharacterized protein LOC103486301 [Cucumis melo] >KAA0044165.1 E3 ubiquitin-protein ligase RNF8-B [Cucumis melo var. makuwa] >TYK24968.1 E3 ubiquitin-protein ligase RNF8-B [Cucumis melo var. makuwa])

HSP 1 Score: 420.2 bits (1079), Expect = 1.1e-113
Identity = 202/220 (91.82%), Postives = 212/220 (96.36%), Query Frame = 0


Query: 1   ASDYPREYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDGKTTMS 60
           ASDYP+EYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDGKTTMS
Sbjct: 23  ASDYPKEYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDGKTTMS 82

Query: 61  IQERKASLKEFYGVIFPSLLQLQGGINDIEDRKQREVYAAKYKRKDQLDRGKITEIDLER 120
           IQERKASLKEFYGVIFPSL+QLQ GI DIE+RKQREVYAAKY+R D L++GKI+EID+ER
Sbjct: 83  IQERKASLKEFYGVIFPSLVQLQRGITDIEERKQREVYAAKYRRTDPLNKGKISEIDMER 142

Query: 121 EEECGICMELNCMVVLPSCNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWIYTGSS 180
           EEECGICMELN  VVLP+CNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWI T SS
Sbjct: 143 EEECGICMELNTKVVLPNCNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWICTSSS 202

Query: 181 EIVDLSFISRENLKRLFMFIDKLPLIVPDPKLISYYLNHY 221
           EIVDLSFISRENLKRLFMFI+KLPLIVPDPKLISYYLNHY
Sbjct: 203 EIVDLSFISRENLKRLFMFINKLPLIVPDPKLISYYLNHY 242

[Close]

BLAST of MS016726 vs. NCBI nr
Match: XP_011651863.1 (E3 ubiquitin-protein ligase AIRP2 [Cucumis sativus] >XP_031738614.1 E3 ubiquitin-protein ligase AIRP2 [Cucumis sativus] >KGN58763.1 hypothetical protein Csa_000946 [Cucumis sativus])

HSP 1 Score: 419.5 bits (1077), Expect = 1.8e-113
Identity = 202/220 (91.82%), Postives = 213/220 (96.82%), Query Frame = 0


Query: 1   ASDYPREYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDGKTTMS 60
           ASDYP+EYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDGKTTMS
Sbjct: 23  ASDYPKEYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDGKTTMS 82

Query: 61  IQERKASLKEFYGVIFPSLLQLQGGINDIEDRKQREVYAAKYKRKDQLDRGKITEIDLER 120
           IQERKASLKEFYGVIFPSL+QLQ GI DIE+RKQREVYAAK+KR D+L++GKI+EIDLER
Sbjct: 83  IQERKASLKEFYGVIFPSLVQLQKGITDIEERKQREVYAAKFKRTDRLNKGKISEIDLER 142

Query: 121 EEECGICMELNCMVVLPSCNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWIYTGSS 180
           EEECGICMELN  VVLP+CNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWI T SS
Sbjct: 143 EEECGICMELNNKVVLPNCNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWICTSSS 202

Query: 181 EIVDLSFISRENLKRLFMFIDKLPLIVPDPKLISYYLNHY 221
           EIVDLSFISR+NLKRLFMFI+KLPLIVPDPKLISYYLNHY
Sbjct: 203 EIVDLSFISRDNLKRLFMFINKLPLIVPDPKLISYYLNHY 242

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BLAST of MS016726 vs. ExPASy Swiss-Prot
Match: Q9M022 (E3 ubiquitin-protein ligase AIRP2 OS=Arabidopsis thaliana OX=3702 GN=AIRP2 PE=1 SV=1)

HSP 1 Score: 335.1 bits (858), Expect = 5.9e-91
Identity = 153/215 (71.16%), Postives = 182/215 (84.65%), Query Frame = 0


Query: 1   ASDYPREYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDGKTTMS 60
           AS+YP EYDG  +QMRLSYSPAA  FLF +QWTDCH AGALGLLRILIYKAY DGKTTMS
Sbjct: 23  ASEYPEEYDGGYVQMRLSYSPAAHLFLFLLQWTDCHFAGALGLLRILIYKAYVDGKTTMS 82

Query: 61  IQERKASLKEFYGVIFPSLLQLQGGINDIEDRKQREVYAAKYKRKDQLDRGKITEIDLER 120
           + ERK S++EFY V+FPSLLQL GGI D+E+RKQ+E+   +Y++KD+ D+GK++EIDLER
Sbjct: 83  LHERKTSIREFYDVLFPSLLQLHGGITDVEERKQKEICDKRYRKKDRTDKGKMSEIDLER 142

Query: 121 EEECGICMELNCMVVLPSCNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWIYTGSS 180
           EEECGIC+E+   VVLP+CNHSMC+ CYR+WR RSQSCPFCR SLKRVNSGDLWIYT S+
Sbjct: 143 EEECGICLEIRNKVVLPTCNHSMCINCYRNWRARSQSCPFCRGSLKRVNSGDLWIYTCSA 202

Query: 181 EIVDLSFISRENLKRLFMFIDKLPLIVPDPKLISY 216
           EI DL  I +ENLKRL ++IDKLPL+  DP L+ Y
Sbjct: 203 EIADLPAIYKENLKRLLIYIDKLPLVTSDPNLVPY 237

[Close]

BLAST of MS016726 vs. ExPASy Swiss-Prot
Match: Q803C1 (E3 ubiquitin-protein ligase rnf8 OS=Danio rerio OX=7955 GN=rnf8 PE=2 SV=1)

HSP 1 Score: 50.8 bits (120), Expect = 2.2e-05
Identity = 35/105 (33.33%), Postives = 49/105 (46.67%), Query Frame = 0


Query: 62  QERKASLKEFYGVIFPSLLQLQGGINDIEDRKQREVYAA---KYKRKDQLDR--GKITEI 121
           ++ + +LKE   VI   L     G  ++   K +E+      K K K Q +    ++TE+
Sbjct: 327 KQLEEALKEHRKVI-EELKHAWQGFKEVLQAKDKELEVTKEEKEKAKAQKEEVVTQMTEV 386

Query: 122 DLEREEECGICMELNCMVVLPSCNHSMCMKCYRSWRTRSQSCPFC 162
            LE E +C IC EL    V  +C HS C  C   WR R   CP C
Sbjct: 387 -LESELQCSICSELFIEAVTLNCAHSFCQHCISEWRNRKDKCPMC 429

[Close]

BLAST of MS016726 vs. ExPASy Swiss-Prot
Match: Q13064 (Probable E3 ubiquitin-protein ligase makorin-3 OS=Homo sapiens OX=9606 GN=MKRN3 PE=1 SV=1)

HSP 1 Score: 48.5 bits (114), Expect = 1.1e-04
Identity = 37/129 (28.68%), Postives = 54/129 (41.86%), Query Frame = 0


Query: 73  GVIF--PSLLQLQGGINDI--------EDRKQREVYAAKYKRKDQLDRGKITEIDLEREE 132
           GV F   S + L G I D+         D  QRE +        + D      +    ++
Sbjct: 250 GVCFRGESCMYLHGDICDMCGLQTLHPMDAAQREEHMRACIEAHEKDMELSFAVQRGMDK 309

Query: 133 ECGICMEL---------NCMVVLPSCNHSMCMKCYRSWRTRSQ-------SCPFCRDSLK 176
            CGICME+             +L +CNHS C++C R WR+  Q       SCP CR + +
Sbjct: 310 VCGICMEVVYEKANPNDRRFGILSNCNHSFCIRCIRRWRSARQFENRIVKSCPQCRVTSE 369

[Close]

BLAST of MS016726 vs. ExPASy Swiss-Prot
Match: Q13434 (Putative E3 ubiquitin-protein ligase makorin-4 OS=Homo sapiens OX=9606 GN=MKRN4P PE=5 SV=1)

HSP 1 Score: 48.1 bits (113), Expect = 1.5e-04
Identity = 23/76 (30.26%), Postives = 38/76 (50.00%), Query Frame = 0


Query: 116 IDLEREEECGICMEL---------NCMVVLPSCNHSMCMKCYRSWRTRSQ-------SCP 175
           +   +++ CGICME+         +   +L +CNH+ C+KC R WR+  +       SCP
Sbjct: 308 VQRSKDKVCGICMEVVYEKANPNEHRFGILSNCNHTFCLKCIRKWRSAKEFESRIVKSCP 367

[Close]

BLAST of MS016726 vs. ExPASy Swiss-Prot
Match: Q60764 (Probable E3 ubiquitin-protein ligase makorin-3 OS=Mus musculus OX=10090 GN=Mkrn3 PE=2 SV=2)

HSP 1 Score: 47.0 bits (110), Expect = 3.2e-04
Identity = 29/101 (28.71%), Postives = 44/101 (43.56%), Query Frame = 0


Query: 91  DRKQREVYAAKYKRKDQLDRGKITEIDLEREEECGICMEL---------NCMVVLPSCNH 150
           D  Q+E +        + D      +    ++ CGICME+             +L SCNH
Sbjct: 314 DAAQQEAHRRACVEAHERDMELSFAVQRSMDKVCGICMEVVYEKADPSDRRFGILFSCNH 373

Query: 151 SMCMKCYRSWRTRSQ-------SCPFCRDSLKRVNSGDLWI 176
           + C++C R WR+ +Q       SCP CR S   V   + W+
Sbjct: 374 TYCLRCIRRWRSATQFENRISKSCPQCRVSSGFVIPSEFWV 414

[Close]

BLAST of MS016726 vs. ExPASy TrEMBL
Match: A0A6J1CVC0 (uncharacterized protein LOC111015127 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111015127 PE=4 SV=1)

HSP 1 Score: 455.7 bits (1171), Expect = 1.1e-124
Identity = 220/220 (100.00%), Postives = 220/220 (100.00%), Query Frame = 0


Query: 1   ASDYPREYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDGKTTMS 60
           ASDYPREYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDGKTTMS
Sbjct: 23  ASDYPREYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDGKTTMS 82

Query: 61  IQERKASLKEFYGVIFPSLLQLQGGINDIEDRKQREVYAAKYKRKDQLDRGKITEIDLER 120
           IQERKASLKEFYGVIFPSLLQLQGGINDIEDRKQREVYAAKYKRKDQLDRGKITEIDLER
Sbjct: 83  IQERKASLKEFYGVIFPSLLQLQGGINDIEDRKQREVYAAKYKRKDQLDRGKITEIDLER 142

Query: 121 EEECGICMELNCMVVLPSCNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWIYTGSS 180
           EEECGICMELNCMVVLPSCNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWIYTGSS
Sbjct: 143 EEECGICMELNCMVVLPSCNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWIYTGSS 202

Query: 181 EIVDLSFISRENLKRLFMFIDKLPLIVPDPKLISYYLNHY 221
           EIVDLSFISRENLKRLFMFIDKLPLIVPDPKLISYYLNHY
Sbjct: 203 EIVDLSFISRENLKRLFMFIDKLPLIVPDPKLISYYLNHY 242

[Close]

BLAST of MS016726 vs. ExPASy TrEMBL
Match: A0A6J1CXK7 (probable E3 ubiquitin-protein ligase makorin-3 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111015127 PE=4 SV=1)

HSP 1 Score: 424.5 bits (1090), Expect = 2.7e-115
Identity = 206/206 (100.00%), Postives = 206/206 (100.00%), Query Frame = 0


Query: 15  MRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDGKTTMSIQERKASLKEFYGV 74
           MRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDGKTTMSIQERKASLKEFYGV
Sbjct: 1   MRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDGKTTMSIQERKASLKEFYGV 60

Query: 75  IFPSLLQLQGGINDIEDRKQREVYAAKYKRKDQLDRGKITEIDLEREEECGICMELNCMV 134
           IFPSLLQLQGGINDIEDRKQREVYAAKYKRKDQLDRGKITEIDLEREEECGICMELNCMV
Sbjct: 61  IFPSLLQLQGGINDIEDRKQREVYAAKYKRKDQLDRGKITEIDLEREEECGICMELNCMV 120

Query: 135 VLPSCNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWIYTGSSEIVDLSFISRENLK 194
           VLPSCNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWIYTGSSEIVDLSFISRENLK
Sbjct: 121 VLPSCNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWIYTGSSEIVDLSFISRENLK 180

Query: 195 RLFMFIDKLPLIVPDPKLISYYLNHY 221
           RLFMFIDKLPLIVPDPKLISYYLNHY
Sbjct: 181 RLFMFIDKLPLIVPDPKLISYYLNHY 206

[Close]

BLAST of MS016726 vs. ExPASy TrEMBL
Match: A0A5D3DMZ7 (E3 ubiquitin-protein ligase RNF8-B OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G00850 PE=4 SV=1)

HSP 1 Score: 420.2 bits (1079), Expect = 5.2e-114
Identity = 202/220 (91.82%), Postives = 212/220 (96.36%), Query Frame = 0


Query: 1   ASDYPREYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDGKTTMS 60
           ASDYP+EYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDGKTTMS
Sbjct: 23  ASDYPKEYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDGKTTMS 82

Query: 61  IQERKASLKEFYGVIFPSLLQLQGGINDIEDRKQREVYAAKYKRKDQLDRGKITEIDLER 120
           IQERKASLKEFYGVIFPSL+QLQ GI DIE+RKQREVYAAKY+R D L++GKI+EID+ER
Sbjct: 83  IQERKASLKEFYGVIFPSLVQLQRGITDIEERKQREVYAAKYRRTDPLNKGKISEIDMER 142

Query: 121 EEECGICMELNCMVVLPSCNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWIYTGSS 180
           EEECGICMELN  VVLP+CNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWI T SS
Sbjct: 143 EEECGICMELNTKVVLPNCNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWICTSSS 202

Query: 181 EIVDLSFISRENLKRLFMFIDKLPLIVPDPKLISYYLNHY 221
           EIVDLSFISRENLKRLFMFI+KLPLIVPDPKLISYYLNHY
Sbjct: 203 EIVDLSFISRENLKRLFMFINKLPLIVPDPKLISYYLNHY 242

[Close]

BLAST of MS016726 vs. ExPASy TrEMBL
Match: A0A1S3B5N9 (uncharacterized protein LOC103486301 OS=Cucumis melo OX=3656 GN=LOC103486301 PE=4 SV=1)

HSP 1 Score: 420.2 bits (1079), Expect = 5.2e-114
Identity = 202/220 (91.82%), Postives = 212/220 (96.36%), Query Frame = 0


Query: 1   ASDYPREYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDGKTTMS 60
           ASDYP+EYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDGKTTMS
Sbjct: 23  ASDYPKEYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDGKTTMS 82

Query: 61  IQERKASLKEFYGVIFPSLLQLQGGINDIEDRKQREVYAAKYKRKDQLDRGKITEIDLER 120
           IQERKASLKEFYGVIFPSL+QLQ GI DIE+RKQREVYAAKY+R D L++GKI+EID+ER
Sbjct: 83  IQERKASLKEFYGVIFPSLVQLQRGITDIEERKQREVYAAKYRRTDPLNKGKISEIDMER 142

Query: 121 EEECGICMELNCMVVLPSCNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWIYTGSS 180
           EEECGICMELN  VVLP+CNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWI T SS
Sbjct: 143 EEECGICMELNTKVVLPNCNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWICTSSS 202

Query: 181 EIVDLSFISRENLKRLFMFIDKLPLIVPDPKLISYYLNHY 221
           EIVDLSFISRENLKRLFMFI+KLPLIVPDPKLISYYLNHY
Sbjct: 203 EIVDLSFISRENLKRLFMFINKLPLIVPDPKLISYYLNHY 242

[Close]

BLAST of MS016726 vs. ExPASy TrEMBL
Match: A0A0A0LA78 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G731740 PE=4 SV=1)

HSP 1 Score: 419.5 bits (1077), Expect = 8.8e-114
Identity = 202/220 (91.82%), Postives = 213/220 (96.82%), Query Frame = 0


Query: 1   ASDYPREYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDGKTTMS 60
           ASDYP+EYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDGKTTMS
Sbjct: 23  ASDYPKEYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDGKTTMS 82

Query: 61  IQERKASLKEFYGVIFPSLLQLQGGINDIEDRKQREVYAAKYKRKDQLDRGKITEIDLER 120
           IQERKASLKEFYGVIFPSL+QLQ GI DIE+RKQREVYAAK+KR D+L++GKI+EIDLER
Sbjct: 83  IQERKASLKEFYGVIFPSLVQLQKGITDIEERKQREVYAAKFKRTDRLNKGKISEIDLER 142

Query: 121 EEECGICMELNCMVVLPSCNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWIYTGSS 180
           EEECGICMELN  VVLP+CNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWI T SS
Sbjct: 143 EEECGICMELNNKVVLPNCNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWICTSSS 202

Query: 181 EIVDLSFISRENLKRLFMFIDKLPLIVPDPKLISYYLNHY 221
           EIVDLSFISR+NLKRLFMFI+KLPLIVPDPKLISYYLNHY
Sbjct: 203 EIVDLSFISRDNLKRLFMFINKLPLIVPDPKLISYYLNHY 242

[Close]

BLAST of MS016726 vs. TAIR 10
Match: AT5G01520.1 (RING/U-box superfamily protein )

HSP 1 Score: 335.1 bits (858), Expect = 4.2e-92
Identity = 153/215 (71.16%), Postives = 182/215 (84.65%), Query Frame = 0


Query: 1   ASDYPREYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDGKTTMS 60
           AS+YP EYDG  +QMRLSYSPAA  FLF +QWTDCH AGALGLLRILIYKAY DGKTTMS
Sbjct: 23  ASEYPEEYDGGYVQMRLSYSPAAHLFLFLLQWTDCHFAGALGLLRILIYKAYVDGKTTMS 82

Query: 61  IQERKASLKEFYGVIFPSLLQLQGGINDIEDRKQREVYAAKYKRKDQLDRGKITEIDLER 120
           + ERK S++EFY V+FPSLLQL GGI D+E+RKQ+E+   +Y++KD+ D+GK++EIDLER
Sbjct: 83  LHERKTSIREFYDVLFPSLLQLHGGITDVEERKQKEICDKRYRKKDRTDKGKMSEIDLER 142

Query: 121 EEECGICMELNCMVVLPSCNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWIYTGSS 180
           EEECGIC+E+   VVLP+CNHSMC+ CYR+WR RSQSCPFCR SLKRVNSGDLWIYT S+
Sbjct: 143 EEECGICLEIRNKVVLPTCNHSMCINCYRNWRARSQSCPFCRGSLKRVNSGDLWIYTCSA 202

Query: 181 EIVDLSFISRENLKRLFMFIDKLPLIVPDPKLISY 216
           EI DL  I +ENLKRL ++IDKLPL+  DP L+ Y
Sbjct: 203 EIADLPAIYKENLKRLLIYIDKLPLVTSDPNLVPY 237

[Close]

BLAST of MS016726 vs. TAIR 10
Match: AT3G47160.1 (RING/U-box superfamily protein )

HSP 1 Score: 314.7 bits (805), Expect = 5.9e-86
Identity = 144/209 (68.90%), Postives = 175/209 (83.73%), Query Frame = 0


Query: 1   ASDYPREYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDGKTTMS 60
           A DYPRE DGA +QMRLSY+PAAQF LF VQWTDCHLAG LGLLR+LIY  Y DGKTTMS
Sbjct: 24  ALDYPREKDGARVQMRLSYTPAAQFLLFLVQWTDCHLAGTLGLLRVLIYMTYADGKTTMS 83

Query: 61  IQERKASLKEFYGVIFPSLLQLQGGINDIEDRKQREVYAAKYKRKDQLDRGKITEIDLER 120
           + ERK S+K+FY VIFPSLLQL+ GI D++DRKQ+EV   +Y+ KD+ ++ K++EID+ER
Sbjct: 84  VYERKTSIKDFYAVIFPSLLQLERGITDLDDRKQKEVCKIRYRNKDETEKVKLSEIDIER 143

Query: 121 EEECGICMELNCMVVLPSCNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWIYTGSS 180
           EEECGICME+N MVVLP+C HS+C+KCYR W  RS+SCPFCRDSLKRVNSGDLW+    S
Sbjct: 144 EEECGICMEMNNMVVLPNCTHSLCIKCYRDWHGRSESCPFCRDSLKRVNSGDLWMLMEKS 203

Query: 181 EIVDLSFISRENLKRLFMFIDKLPLIVPD 210
           + V++  I REN KRLF++I+KLPL+VPD
Sbjct: 204 DTVNMYTIERENKKRLFVYIEKLPLVVPD 232

[Close]

BLAST of MS016726 vs. TAIR 10
Match: AT3G47160.2 (RING/U-box superfamily protein )

HSP 1 Score: 314.7 bits (805), Expect = 5.9e-86
Identity = 144/209 (68.90%), Postives = 175/209 (83.73%), Query Frame = 0


Query: 1   ASDYPREYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDGKTTMS 60
           A DYPRE DGA +QMRLSY+PAAQF LF VQWTDCHLAG LGLLR+LIY  Y DGKTTMS
Sbjct: 36  ALDYPREKDGARVQMRLSYTPAAQFLLFLVQWTDCHLAGTLGLLRVLIYMTYADGKTTMS 95

Query: 61  IQERKASLKEFYGVIFPSLLQLQGGINDIEDRKQREVYAAKYKRKDQLDRGKITEIDLER 120
           + ERK S+K+FY VIFPSLLQL+ GI D++DRKQ+EV   +Y+ KD+ ++ K++EID+ER
Sbjct: 96  VYERKTSIKDFYAVIFPSLLQLERGITDLDDRKQKEVCKIRYRNKDETEKVKLSEIDIER 155

Query: 121 EEECGICMELNCMVVLPSCNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWIYTGSS 180
           EEECGICME+N MVVLP+C HS+C+KCYR W  RS+SCPFCRDSLKRVNSGDLW+    S
Sbjct: 156 EEECGICMEMNNMVVLPNCTHSLCIKCYRDWHGRSESCPFCRDSLKRVNSGDLWMLMEKS 215

Query: 181 EIVDLSFISRENLKRLFMFIDKLPLIVPD 210
           + V++  I REN KRLF++I+KLPL+VPD
Sbjct: 216 DTVNMYTIERENKKRLFVYIEKLPLVVPD 244

[Close]

BLAST of MS016726 vs. TAIR 10
Match: AT5G58787.1 (RING/U-box superfamily protein )

HSP 1 Score: 302.0 bits (772), Expect = 4.0e-82
Identity = 141/209 (67.46%), Postives = 173/209 (82.78%), Query Frame = 0


Query: 1   ASDYPREYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDGKTTMS 60
           A DYPRE DGA +QMRLSYSP AQFFLF VQWTDC LAG LGLLR+LIY  Y DGKTTMS
Sbjct: 24  ALDYPREKDGARVQMRLSYSPTAQFFLFLVQWTDCKLAGFLGLLRVLIYMTYADGKTTMS 83

Query: 61  IQERKASLKEFYGVIFPSLLQLQGGINDIEDRKQREVYAAKYKRKDQLDRGKITEIDLER 120
           + ERKAS++EF  VI PSL QLQ G+ DI+D KQ+EV   +Y++KD+    +++EI++ER
Sbjct: 84  VYERKASIREFQAVILPSLSQLQRGVTDIDDSKQKEVCKMRYRKKDE---SEMSEIEIER 143

Query: 121 EEECGICMELNCMVVLPSCNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWIYTGSS 180
           EEECGICME+N  VVLP+C HS+C+KCYR WR RSQSCPFCRDSLKRV+SGDLW++   +
Sbjct: 144 EEECGICMEMNSKVVLPNCTHSLCIKCYRDWRGRSQSCPFCRDSLKRVDSGDLWMFLDQN 203

Query: 181 EIVDLSFISRENLKRLFMFIDKLPLIVPD 210
           + V+L+ I+REN KRLFM+I+KLPL+VPD
Sbjct: 204 DTVNLTAIARENQKRLFMYIEKLPLVVPD 229

[Close]

BLAST of MS016726 vs. TAIR 10
Match: AT5G58787.2 (RING/U-box superfamily protein )

HSP 1 Score: 275.0 bits (702), Expect = 5.2e-74
Identity = 132/209 (63.16%), Postives = 163/209 (77.99%), Query Frame = 0


Query: 1   ASDYPREYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDGKTTMS 60
           A DYPRE DGA +QMRLSYSP AQFFLF VQWTDC LAG LGLLR+LIY  Y DGKTTMS
Sbjct: 24  ALDYPREKDGARVQMRLSYSPTAQFFLFLVQWTDCKLAGFLGLLRVLIYMTYADGKTTMS 83

Query: 61  IQERKASLKEFYGVIFPSLLQLQGGINDIEDRKQREVYAAKYKRKDQLDRGKITEIDLER 120
           + ERKAS++EF                DI+D KQ+EV   +Y++KD+    +++EI++ER
Sbjct: 84  VYERKASIREF---------------QDIDDSKQKEVCKMRYRKKDE---SEMSEIEIER 143

Query: 121 EEECGICMELNCMVVLPSCNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWIYTGSS 180
           EEECGICME+N  VVLP+C HS+C+KCYR WR RSQSCPFCRDSLKRV+SGDLW++   +
Sbjct: 144 EEECGICMEMNSKVVLPNCTHSLCIKCYRDWRGRSQSCPFCRDSLKRVDSGDLWMFLDQN 203

Query: 181 EIVDLSFISRENLKRLFMFIDKLPLIVPD 210
           + V+L+ I+REN KRLFM+I+KLPL+VPD
Sbjct: 204 DTVNLTAIARENQKRLFMYIEKLPLVVPD 214

The following BLAST results are available for this feature:

BLAST of MS016726 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022145744.1	2.3e-124	100.00	uncharacterized protein LOC111015127 isoform X1 [Momordica charantia] >XP_022145...	[more]
XP_022145747.1	5.7e-115	100.00	probable E3 ubiquitin-protein ligase makorin-3 isoform X2 [Momordica charantia]	[more]
XP_038904042.1	2.2e-114	92.73	E3 ubiquitin-protein ligase AIRP2 isoform X1 [Benincasa hispida]	[more]
XP_008442427.1	1.1e-113	91.82	PREDICTED: uncharacterized protein LOC103486301 [Cucumis melo] >KAA0044165.1 E3 ...	[more]
XP_011651863.1	1.8e-113	91.82	E3 ubiquitin-protein ligase AIRP2 [Cucumis sativus] >XP_031738614.1 E3 ubiquitin...	[more]

BLAST of MS016726 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9M022	5.9e-91	71.16	E3 ubiquitin-protein ligase AIRP2 OS=Arabidopsis thaliana OX=3702 GN=AIRP2 PE=1 ...	[more]
Q803C1	2.2e-05	33.33	E3 ubiquitin-protein ligase rnf8 OS=Danio rerio OX=7955 GN=rnf8 PE=2 SV=1	[more]
Q13064	1.1e-04	28.68	Probable E3 ubiquitin-protein ligase makorin-3 OS=Homo sapiens OX=9606 GN=MKRN3 ...	[more]
Q13434	1.5e-04	30.26	Putative E3 ubiquitin-protein ligase makorin-4 OS=Homo sapiens OX=9606 GN=MKRN4P...	[more]
Q60764	3.2e-04	28.71	Probable E3 ubiquitin-protein ligase makorin-3 OS=Mus musculus OX=10090 GN=Mkrn3...	[more]

BLAST of MS016726 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CVC0	1.1e-124	100.00	uncharacterized protein LOC111015127 isoform X1 OS=Momordica charantia OX=3673 G...	[more]
A0A6J1CXK7	2.7e-115	100.00	probable E3 ubiquitin-protein ligase makorin-3 isoform X2 OS=Momordica charantia...	[more]
A0A5D3DMZ7	5.2e-114	91.82	E3 ubiquitin-protein ligase RNF8-B OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]
A0A1S3B5N9	5.2e-114	91.82	uncharacterized protein LOC103486301 OS=Cucumis melo OX=3656 GN=LOC103486301 PE=...	[more]
A0A0A0LA78	8.8e-114	91.82	RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G731740 P...	[more]

BLAST of MS016726 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G01520.1	4.2e-92	71.16	RING/U-box superfamily protein	[more]
AT3G47160.1	5.9e-86	68.90	RING/U-box superfamily protein	[more]
AT3G47160.2	5.9e-86	68.90	RING/U-box superfamily protein	[more]
AT5G58787.1	4.0e-82	67.46	RING/U-box superfamily protein	[more]
AT5G58787.2	5.2e-74	63.16	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 124..161 e-value: 1.1E-5 score: 34.9
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 124..162 score: 11.751628
None	No IPR available	PFAM	PF13920	zf-C3HC4_3	coord: 121..167 e-value: 4.2E-8 score: 32.9
None	No IPR available	PANTHER	PTHR15315	RING FINGER PROTEIN 41, 151	coord: 1..217
None	No IPR available	PANTHER	PTHR15315:SF31	E3 UBIQUITIN-PROTEIN LIGASE AIRP2	coord: 1..217
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 107..167
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 97..170 e-value: 8.6E-14 score: 52.6
IPR017907	Zinc finger, RING-type, conserved site	PROSITE	PS00518	ZF_RING_1	coord: 139..148

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS016726.1	MS016726.1	mRNA