MS016715 (gene) Bitter gourd (TR) v1

Overview


Name	MS016715
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	AdoMet-dependent rRNA methyltransferase spb1
Location	scaffold563: 259026 .. 263252 (-)
RNA-Seq Expression	MS016715
Synteny	MS016715

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

CAGGATATATACTACCGTAAAGCAAAAGAAGAAGGTTGGCGAGCTCGAAGTGCATTTAAACTTCTTCAGATAGATGAGGAATTTAACATATTTGAAGGTTTGCTCTTGAATTGGGCACCTCTATCTCTCTCTTAAAACATTCTGACATTTATGCTAGAGAAGTCAACCACTGAGATGGTCCAGATGAATATTAAAGATTCCTTTTTCAAGAATACTCTTTTCCTTTGTTTTTTAGGAGTTTTGTCACCTGGAGAAGTCATTCTTTCCTCATGAACAATGTTGCAACATTTTGGATTAACCTCCATAACATAATTGTTTTCCAGAATCCTCCTAAAATCTGTGTTATTTGTCATATCATGTATAAGTCTTGTAAATACTTCTAAGTAACAACGAAATTTCAAATGCAGGAGTCAAGCGAGTGGTGGATTTATGTGCAGCCCCTGGTAGCTGGAGCCAGGTAATCTCTTTTGGGTATCTTTTTGTTGCAATCAGTGTGTCAAACTAGTAAAATACAACTATTTATAACAAGAATTCTTCTTCGAGTGATCAACATCGTAAGCATTTTTTTTTCCTTTGTTGAATAGCATGTCTCTATATATGCGGGGCAAGGCTTGCCTCCCTTTATTGCACCCTGCATCTTCGTGCGCTTTTCAAAAGTAATGATTGCATTAGTCGTTCTTGAAGAAGGGAAGAAAATAACTTGAATCCCTTAATTTTTAAGCACTTTAATAGAACATTTATTTTAAATTTTTGTTGTCTCATTTTCCATTACATTATAGTTTTGTTTTTTTATATATTAAACCCCATAGGCTATAAGCAATGTCTTATATGTTGCTCCAAGCCTGCAACTATCCCTCATAAATATGATTTTTTGGCTAACTATTTCCAAATTATCTTAACCTTTTCTCCTCTACTAATCATATGTAGGTCCTTAGTAGGAAATTATATCTCCCAGCTAAGCTTTCTTCTGTTTCTAAGTAAGTTATGGTAGATTATTATAAAAAATGTAACTGTTTCCTTCCCGTCCTTCTTTCTGTTTGCACCTTGTGATTTCCCTATTTCATTGATTCTCTAGCTTGCTTCACAGGGATGGCGATCTTCCACTTATTGTTGCTATCGATTTGCAACCGATGGCCCCAATTGAGGGTGTTATTCAAGTACAGGGTGATATAACAAATGCTAGGACCGCTGAAGTGGTAAGTAGAATCATGTACACAGTATTTTTAAACATTTGTGTAGAAAAATTTTGAAGTATTGAATATTCTGTCAACTAAACGAATCAGATTCTATTCACAGATTTGGTTTGTTTTGAAATTTCTATTTGCGATAATTTCTCCTATGCTTTTCTCCATATTGGATTTTGTTCTCTTTTGAACGAGCAAACAAGTCTGTTAGAAGTCTTTCATTTTATTCAACAATTTGATCACATATTATATTGTCATATTTTTTAATGTATGGCTTATCAAATATTTACTTTTTCCAGGTCATTAGGCATTTTGATGGGTGTAAGGCTGACCTGGTGGTTTGTGATGGTGCTCCTGATGGTAGATACTTATACTTGTATTTTCTTTTTTTAATTGTATGTTGAGATTTTACATGATGACATTTTTGTTACCAATTACGTGTTCATTTGTAGTTACTGGTCTTCATGACATGGACGAATTTGTTCAGTCCCAGCTCATACTAGCTGTAAGTACTGTGTTTTTCTACGGATACTGATTGCTTCTCGTGGTGAGGCTGATGCATAGTTTAAGATAATTGAAAATGCGTGATGTATATGTTGGCAATGATGTTGCTTCAGCTCACATTTACAGTGTGTGTGGAAAGGATACAAAGGAATAACCAACAAGAAGCATTAATGAGTCGAATTGTTTGAACCTACAAATAGAAATTAACGTGTAACAAGTGAGCAAGGTAGATCCCTTTAGGTAAAATGTGATTGCTGTATTCCGAAATTATATTGAACCAGTCAAAGGGTTTTGCTATTATATGTTGCCTGCACTTGTTGAGCCTTTTCTTCTATGGATTATGTTCCACCTAAGTGAATTTTTTAGCTTCCATCACTGTCCTGTGCTGCCTCTTCTCTCTCTCTTCCTTGAGCTAGTTTCAATATAGTGGGACTGGTTCATACATAAGGTTCACATAGCTGAGCCACGCTTTCCTCTTTCTTTTTTTTTTTTTTTTTNNNNNNNNNTTTTGCAGGGTCTAACAATAGTTACCCACGTTCTTAGAGAAGGTGGTAAGTTCATAGCAAAGATATTCCGTGGAAAGGATACAAGCCTTTTGTATTGTCAGGTATATGTTATGATTGTTACAGTACCCATTCAAAGAAACATGTAATATGCTCAAGTGACAAATTTATGGCGTTTTCACAAACTGCAAAAAATCTCTTCTCCCTTCTACTTTATTTTGTTAGTCAGTCAAGTTTGAAGGTTTGCCGTGAGCTCTTCTGTTGCTTGTGATTATTGCGTTGATGTGCGTATTCCTTTTAGATATGTTTAGCATTAGTAGAACTTGTTAAATATAATGATGAGCTACATAAGAATCTTAATATAAGAGAGGTGTTTGTTGCTTCATACATGAAGTCGTCAATGATGTCATACATTTTGGTGTTTTTTGTGGCAGCTGAAGTTATTTTTCCCTGTAGTGACTTTTGCAAAACCAAAAAGCAGCCGCAACTCCAGTATAGGTACCCTTTTTCCTACTTTTTTCTTTATTGGTAGCTCTATAGGTTCATCTTTCGTTTATAATTGAACTTCTTTCCTGAAAATGATACCCTCCTTATTGAAATTGCCTTTTTATGTCTTTGTAGTGTCTAGTTCATAGGATTCTTGCGCATTGATGTTCAGATACCCATTTTACTTAGATTCCTTCAATGAGCTTTAGATTGTTATCGTTTAATACTAGGTGGGACTGGTGGCCCGCCAACTGCAAATTTGGAGTTAGCTCATTTTTGTGGTGACATCTGTAAAATAAAAAAACATGTAAAAGAGGACAAAAACAGCTAATGGTTAAATTATTAAATTCAACTCTAAACTATGATGGATGTGTCTTTGAACATCCTTGAAGGGTTCATTTGATTCCAATAAGTCTATTTTGTTAAAATTGTTACATAAATGTATGACAAATCAAACTAAATAGATTAACCAGTGATGATTTAATATGACGTAACAAGTGATGATTTAATATGACGTACATGAAAAGGTAGGCCTATTAGATTGAATAATGGTAGATTGGCCCAAAAATTTGATGGGAGCAGGGGCGGGAAATACATCTTAAACGAATTTTTTTATTTGACTACCCTAGTAGCTTCTTTTGTGTGTCACATCCTACCAAATCATTATTCATTAGTTCAATCAACGTTTCATGTCAACATTTAAACAAACAACAGTGTTAACTATAAAAGTTTATTGAAAAGCAAAATCGAAGGTTCAAGGACATAATTGAAACTTTTAGAAATGGAGAGAGTAAAGTTCAAGAACTAAATTAGTAATAATGTTACCAATTAAATTACTGCTATTTCTAAGTTCCAGTGCTTTCATTTAGTCACGTAGGTGGAGCCACGGTGAAAACTTGTAAACAAAGTTCAGATTATGATTTAACTTTATGTTGATGTTGAAGCTCATACATGTACTGGGTGTGAATGCAGAGGCATTTGCAGTTTGTGAGAACTATTCCCCTCCTGAAGGTTTCAACCCAAAAGATCTGCATCGCCTACTAGAAAAGGTGGGAAGTCCGTCAGGAGGAGATGACTTAGGTAAACTCAATGTTCTGCCTTGATTTTGGAGTTTACATATCGGTAGACACATCCTAAGAATCAAGCATGTTCTTATTTCTTATCCTGCTTTTAAAAATTAATTTGTTTTACAGCTGGCAATGACCAAGCTGTCTTCTTGTCTTCTGCCTTTTTAAAATTAGTGTCTCTGGTTTTGATGAGTCAATTCCCTGGTATTTTTTGTTACACAAGAGGAAGTTATAGCATTGCTATTTTGCCCCATCGTGTCCCTCTCAGATTGCAGTAGTGGGTGGCTGGAAGGTCCCAATAAAGTTTACATACCGTTTCTTGCCTGTGGGGACCTCAGTGGATATGACTCCGACCGTTCATACCCACTGCCCAGAGGTGCCGAGGGCGCTTATCGGAGCTTAGATCCTGTGCAGCCCCCGATCGCCCCTCCTTACAAGAGGGCTCTAGAAATGAAGAAAGCTTCCAGTCAAGGAGGTCGAGAA

mRNA sequence

CAGGATATATACTACCGTAAAGCAAAAGAAGAAGGTTGGCGAGCTCGAAGTGCATTTAAACTTCTTCAGATAGATGAGGAATTTAACATATTTGAAGGAGTCAAGCGAGTGGTGGATTTATGTGCAGCCCCTGGTAGCTGGAGCCAGGTAATCTCTTTTGGGTATCTTTTTGTTGCAATCAGTGTCTTGCTTCACAGGGATGGCGATCTTCCACTTATTGTTGCTATCGATTTGCAACCGATGGCCCCAATTGAGGGTGTTATTCAAGTACAGGGTGATATAACAAATGCTAGGACCGCTGAAGTGGTCATTAGGCATTTTGATGGGTGTAAGGCTGACCTGGTGGTTTGTGATGGTGCTCCTGATGTTACTGGTCTTCATGACATGGACGAATTTGTTCAGTCCCAGCTCATACTAGCTGGTCTAACAATAGTTACCCACGTTCTTAGAGAAGGTGGTAAGTTCATAGCAAAGATATTCCGTGGAAAGGATACAAGCCTTTTGTATTGTCAGCTGAAGTTATTTTTCCCTGTAGTGACTTTTGCAAAACCAAAAAGCAGCCGCAACTCCAGTATAGAGGCATTTGCAGTTTGTGAGAACTATTCCCCTCCTGAAGGTTTCAACCCAAAAGATCTGCATCGCCTACTAGAAAAGGTGGGAAGTCCGTCAGGAGGAGATGACTTAGATTGCAGTAGTGGGTGGCTGGAAGGTCCCAATAAAGTTTACATACCGTTTCTTGCCTGTGGGGACCTCAGTGGATATGACTCCGACCGTTCATACCCACTGCCCAGAGGTGCCGAGGGCGCTTATCGGAGCTTAGATCCTGTGCAGCCCCCGATCGCCCCTCCTTACAAGAGGGCTCTAGAAATGAAGAAAGCTTCCAGTCAAGGAGGTCGAGAA

Coding sequence (CDS)

CAGGATATATACTACCGTAAAGCAAAAGAAGAAGGTTGGCGAGCTCGAAGTGCATTTAAACTTCTTCAGATAGATGAGGAATTTAACATATTTGAAGGAGTCAAGCGAGTGGTGGATTTATGTGCAGCCCCTGGTAGCTGGAGCCAGGTAATCTCTTTTGGGTATCTTTTTGTTGCAATCAGTGTCTTGCTTCACAGGGATGGCGATCTTCCACTTATTGTTGCTATCGATTTGCAACCGATGGCCCCAATTGAGGGTGTTATTCAAGTACAGGGTGATATAACAAATGCTAGGACCGCTGAAGTGGTCATTAGGCATTTTGATGGGTGTAAGGCTGACCTGGTGGTTTGTGATGGTGCTCCTGATGTTACTGGTCTTCATGACATGGACGAATTTGTTCAGTCCCAGCTCATACTAGCTGGTCTAACAATAGTTACCCACGTTCTTAGAGAAGGTGGTAAGTTCATAGCAAAGATATTCCGTGGAAAGGATACAAGCCTTTTGTATTGTCAGCTGAAGTTATTTTTCCCTGTAGTGACTTTTGCAAAACCAAAAAGCAGCCGCAACTCCAGTATAGAGGCATTTGCAGTTTGTGAGAACTATTCCCCTCCTGAAGGTTTCAACCCAAAAGATCTGCATCGCCTACTAGAAAAGGTGGGAAGTCCGTCAGGAGGAGATGACTTAGATTGCAGTAGTGGGTGGCTGGAAGGTCCCAATAAAGTTTACATACCGTTTCTTGCCTGTGGGGACCTCAGTGGATATGACTCCGACCGTTCATACCCACTGCCCAGAGGTGCCGAGGGCGCTTATCGGAGCTTAGATCCTGTGCAGCCCCCGATCGCCCCTCCTTACAAGAGGGCTCTAGAAATGAAGAAAGCTTCCAGTCAAGGAGGTCGAGAA

Protein sequence

QDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVISFGYLFVAISVLLHRDGDLPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIRHFDGCKADLVVCDGAPDVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQLKLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDCSSGWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGAYRSLDPVQPPIAPPYKRALEMKKASSQGGRE

Homology

[Close]

BLAST of MS016715 vs. NCBI nr
Match: XP_022145642.1 (putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase isoform X1 [Momordica charantia])

HSP 1 Score: 580.1 bits (1494), Expect = 1.1e-161
Identity = 287/303 (94.72%), Postives = 290/303 (95.71%), Query Frame = 0


Query: 1   QDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVISFGYLFVA- 60
           +DIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQV+S      A 
Sbjct: 9   RDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLSRKLYLPAK 68

Query: 61  ---ISVLLHRDGDLPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIRHFDGCKADLVV 120
              +S LLHRDGDLPLIVAIDLQPMAPIEGVIQVQGDITNARTA VVIRHFDGCKADLVV
Sbjct: 69  LSSVSNLLHRDGDLPLIVAIDLQPMAPIEGVIQVQGDITNARTAGVVIRHFDGCKADLVV 128

Query: 121 CDGAPDVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQLKLFF 180
           CDGAPDVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQLKLFF
Sbjct: 129 CDGAPDVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQLKLFF 188

Query: 181 PVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDCSSGWLE 240
           PVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPS GDDLDCSSGWLE
Sbjct: 189 PVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSXGDDLDCSSGWLE 248

Query: 241 GPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGAYRSLDPVQPPIAPPYKRALEMKKASSQ 300
           GPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGAYRSLDPVQPPIAPPYKRALEMKKASSQ
Sbjct: 249 GPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGAYRSLDPVQPPIAPPYKRALEMKKASSQ 308

[Close]

BLAST of MS016715 vs. NCBI nr
Match: XP_022145643.1 (putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase isoform X2 [Momordica charantia])

HSP 1 Score: 573.5 bits (1477), Expect = 1.0e-159
Identity = 282/299 (94.31%), Postives = 286/299 (95.65%), Query Frame = 0


Query: 1   QDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVISFGYLFVAI 60
           +DIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQV+S      A 
Sbjct: 9   RDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLSRKLYLPAK 68

Query: 61  SVLLHRDGDLPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIRHFDGCKADLVVCDGA 120
              + +DGDLPLIVAIDLQPMAPIEGVIQVQGDITNARTA VVIRHFDGCKADLVVCDGA
Sbjct: 69  LSSVSKDGDLPLIVAIDLQPMAPIEGVIQVQGDITNARTAGVVIRHFDGCKADLVVCDGA 128

Query: 121 PDVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQLKLFFPVVT 180
           PDVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQLKLFFPVVT
Sbjct: 129 PDVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQLKLFFPVVT 188

Query: 181 FAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDCSSGWLEGPNK 240
           FAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPS GDDLDCSSGWLEGPNK
Sbjct: 189 FAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSXGDDLDCSSGWLEGPNK 248

Query: 241 VYIPFLACGDLSGYDSDRSYPLPRGAEGAYRSLDPVQPPIAPPYKRALEMKKASSQGGR 300
           VYIPFLACGDLSGYDSDRSYPLPRGAEGAYRSLDPVQPPIAPPYKRALEMKKASSQGGR
Sbjct: 249 VYIPFLACGDLSGYDSDRSYPLPRGAEGAYRSLDPVQPPIAPPYKRALEMKKASSQGGR 307

[Close]

BLAST of MS016715 vs. NCBI nr
Match: KAG6580486.1 (putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 569.3 bits (1466), Expect = 1.9e-158
Identity = 281/300 (93.67%), Postives = 285/300 (95.00%), Query Frame = 0


Query: 1   QDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVISFGYLFVAI 60
           +DIYYRKAKEEGWRARSAFKLLQID+EFNIFEGVKRVVDLCAAPGSWSQV+S      A 
Sbjct: 29  RDIYYRKAKEEGWRARSAFKLLQIDDEFNIFEGVKRVVDLCAAPGSWSQVLSRKLYLPAK 88

Query: 61  SVLLHRDGDLPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIRHFDGCKADLVVCDGA 120
                +DGD PLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIRHFDGCKADLVVCDGA
Sbjct: 89  LSPDSKDGDHPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIRHFDGCKADLVVCDGA 148

Query: 121 PDVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQLKLFFPVVT 180
           PDVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQLKLFFPVVT
Sbjct: 149 PDVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQLKLFFPVVT 208

Query: 181 FAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDCSSGWLEGPNK 240
           FAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDCSSGWLEGPNK
Sbjct: 209 FAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDCSSGWLEGPNK 268

Query: 241 VYIPFLACGDLSGYDSDRSYPLPRGAEGAYRSLDPVQPPIAPPYKRALEMKKASSQGGRE 300
           VYIPFLACGDLSGYDSDRSYPLPRGAEG YRSLDPVQPPIAPPYKRALEMKKASSQG RE
Sbjct: 269 VYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKKASSQGIRE 328

[Close]

BLAST of MS016715 vs. NCBI nr
Match: XP_022934133.1 (putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase [Cucurbita moschata] >XP_022934134.1 putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase [Cucurbita moschata] >XP_023528668.1 putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase [Cucurbita pepo subsp. pepo] >XP_023528669.1 putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase [Cucurbita pepo subsp. pepo] >KAG7017237.1 putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 569.3 bits (1466), Expect = 1.9e-158
Identity = 281/300 (93.67%), Postives = 285/300 (95.00%), Query Frame = 0


Query: 1   QDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVISFGYLFVAI 60
           +DIYYRKAKEEGWRARSAFKLLQID+EFNIFEGVKRVVDLCAAPGSWSQV+S      A 
Sbjct: 9   RDIYYRKAKEEGWRARSAFKLLQIDDEFNIFEGVKRVVDLCAAPGSWSQVLSRKLYLPAK 68

Query: 61  SVLLHRDGDLPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIRHFDGCKADLVVCDGA 120
                +DGD PLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIRHFDGCKADLVVCDGA
Sbjct: 69  LSPDSKDGDHPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIRHFDGCKADLVVCDGA 128

Query: 121 PDVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQLKLFFPVVT 180
           PDVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQLKLFFPVVT
Sbjct: 129 PDVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQLKLFFPVVT 188

Query: 181 FAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDCSSGWLEGPNK 240
           FAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDCSSGWLEGPNK
Sbjct: 189 FAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDCSSGWLEGPNK 248

Query: 241 VYIPFLACGDLSGYDSDRSYPLPRGAEGAYRSLDPVQPPIAPPYKRALEMKKASSQGGRE 300
           VYIPFLACGDLSGYDSDRSYPLPRGAEG YRSLDPVQPPIAPPYKRALEMKKASSQG RE
Sbjct: 249 VYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKKASSQGIRE 308

[Close]

BLAST of MS016715 vs. NCBI nr
Match: XP_038903231.1 (putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase [Benincasa hispida])

HSP 1 Score: 568.9 bits (1465), Expect = 2.5e-158
Identity = 281/300 (93.67%), Postives = 285/300 (95.00%), Query Frame = 0


Query: 1   QDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVISFGYLFVAI 60
           +DIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGV+RVVDLCAAPGSWSQV+S      A 
Sbjct: 9   RDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVERVVDLCAAPGSWSQVLSRKLHLPAK 68

Query: 61  SVLLHRDGDLPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIRHFDGCKADLVVCDGA 120
                +DGDLPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIRHFDGCKADLVVCDGA
Sbjct: 69  LSPDSKDGDLPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIRHFDGCKADLVVCDGA 128

Query: 121 PDVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQLKLFFPVVT 180
           PDVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQLKLFFPVVT
Sbjct: 129 PDVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQLKLFFPVVT 188

Query: 181 FAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDCSSGWLEGPNK 240
           FAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDCSSGWLEGPNK
Sbjct: 189 FAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDCSSGWLEGPNK 248

Query: 241 VYIPFLACGDLSGYDSDRSYPLPRGAEGAYRSLDPVQPPIAPPYKRALEMKKASSQGGRE 300
           VYIPFLACGDLSGYDSDRSYPLPRGAEG YR LDPVQPPIAPPYKRALEMKKASSQG RE
Sbjct: 249 VYIPFLACGDLSGYDSDRSYPLPRGAEGTYRCLDPVQPPIAPPYKRALEMKKASSQGIRE 308

[Close]

BLAST of MS016715 vs. ExPASy Swiss-Prot
Match: Q9UET6 (Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Homo sapiens OX=9606 GN=FTSJ1 PE=1 SV=2)

HSP 1 Score: 341.3 bits (874), Expect = 1.1e-92
Identity = 168/292 (57.53%), Postives = 208/292 (71.23%), Query Frame = 0


Query: 1   QDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVISFGYLFVAI 60
           +D+YYR AKE GWRARSAFKLLQ+D+EF +F+GV R VDLCAAPGSWSQV         +
Sbjct: 9   RDVYYRLAKENGWRARSAFKLLQLDKEFQLFQGVTRAVDLCAAPGSWSQV---------L 68

Query: 61  SVLLHRDGDLPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIRHFDGCKADLVVCDGA 120
           S  +   G    +VA+DLQ MAP+ GV+Q+QGDIT   TA+ +I+HF GC ADLVVCDGA
Sbjct: 69  SQKIGGQGS-GHVVAVDLQAMAPLPGVVQIQGDITQLSTAKEIIQHFKGCPADLVVCDGA 128

Query: 121 PDVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQLKLFFPVVT 180
           PDVTGLHD+DE++Q+QL+LA L I THVL+ GG F+AKIFRG+D +LLY QL++FF  V 
Sbjct: 129 PDVTGLHDVDEYMQAQLLLAALNIATHVLKPGGCFVAKIFRGRDVTLLYSQLQVFFSSVL 188

Query: 181 FAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDCSSGWLEGPNK 240
            AKP+SSRNSSIEAFAVC+ Y PPEGF P     LL+    P            L+GP +
Sbjct: 189 CAKPRSSRNSSIEAFAVCQGYDPPEGFIPDLSKPLLDHSYDPDFNQ--------LDGPTR 248

Query: 241 VYIPFLACGDLSGYDSDRSYPLPRGAEGAYRSLDPVQPPIAPPYKRALEMKK 293
           + +PF+ CGDLS YDSDRSYPL       Y+   P QPPI+PPY+ A  +K+
Sbjct: 249 IIVPFVTCGDLSSYDSDRSYPLDLEGGSEYKYTPPTQPPISPPYQEACTLKR 282

[Close]

BLAST of MS016715 vs. ExPASy Swiss-Prot
Match: O36015 (Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC4F10.03c PE=3 SV=1)

HSP 1 Score: 310.5 bits (794), Expect = 2.1e-83
Identity = 161/291 (55.33%), Postives = 208/291 (71.48%), Query Frame = 0


Query: 1   QDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVISFGYLFVAI 60
           +D YYR AKE+GWRARSAFKLLQ++E+FN+FEG KRVVDLCAAPGSWSQV+S   L   I
Sbjct: 9   RDAYYRLAKEQGWRARSAFKLLQLNEQFNLFEGAKRVVDLCAAPGSWSQVLS-RELLKNI 68

Query: 61  SVLLHRDGDLPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIRHFDGCKADLVVCDGA 120
              +  D + P+IVA+DLQPMAPI+GV  +Q DIT+  T  +++ HF    ADLVV DGA
Sbjct: 69  DTSIAAD-EKPMIVAVDLQPMAPIDGVCTLQLDITHPNTLSIILSHFGNEPADLVVSDGA 128

Query: 121 PDVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQLKLFFPVVT 180
           PDVTGLHD+DE++Q+Q++LA   +   VL+ GGKF+AKIFRG+D SLLY QL+L F  V+
Sbjct: 129 PDVTGLHDLDEYIQAQILLAAFNLAVCVLKPGGKFVAKIFRGRDVSLLYSQLRLMFRKVS 188

Query: 181 FAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDCSSGWLEGPNK 240
            AKP+SSR SSIE+F VCE+++PP  F P DL + L  V  P+   ++            
Sbjct: 189 CAKPRSSRASSIESFVVCEDFNPPSNFQP-DLTKPL-CVIDPTNAHEI------------ 248

Query: 241 VYIPFLACGDLSGYDSDRSYPLPRGAEGAYRSLDPVQPPIAPPYKRALEMK 292
              PF+ACGDL GYD+D +YP+    + A  +LD +QPP APPYKRA+E+K
Sbjct: 249 --APFIACGDLDGYDADATYPVEINMKKA--TLDVIQPPTAPPYKRAIELK 279

[Close]

BLAST of MS016715 vs. ExPASy Swiss-Prot
Match: Q22031 (Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Caenorhabditis elegans OX=6239 GN=R74.7 PE=3 SV=3)

HSP 1 Score: 306.2 bits (783), Expect = 4.0e-82
Identity = 163/304 (53.62%), Postives = 204/304 (67.11%), Query Frame = 0


Query: 1   QDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVISFGYLFVAI 60
           +DIYYR AKE  WRARSAFKL+QID+EF I +GV+R VDLCAAPGSWSQV         +
Sbjct: 9   RDIYYRLAKENKWRARSAFKLMQIDDEFQILKGVRRAVDLCAAPGSWSQV---------L 68

Query: 61  SVLLHRDGDLPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIRHFDGCKADLVVCDGA 120
           S  L+ +     IVAIDLQPMAPI GVIQ+QGDIT+  TA  VI+HF G K+D+V+CDGA
Sbjct: 69  SKRLYEEDQEAKIVAIDLQPMAPIPGVIQLQGDITSVDTANQVIKHFSGEKSDIVICDGA 128

Query: 121 PDVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQLKLFFPVVT 180
           PDVTG+H +DEF+Q++LILA   I +HVL+EGG F+AKIFR +++SLLY Q+K +F  V 
Sbjct: 129 PDVTGIHSLDEFMQAELILAAFNITSHVLKEGGNFLAKIFRSRNSSLLYAQMKKYFKKVY 188

Query: 181 FAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDCSSGWLEGPNK 240
            AKP+SSR SS EAF +C +YSPPEGF P              G   LD +      P+ 
Sbjct: 189 LAKPRSSRQSSCEAFVLCLDYSPPEGFVP------------TMGKTSLDATDASAISPD- 248

Query: 241 VYIPFLACGDLSGYDSDRSYPL------PRG-----AEGAYRSLDPVQPPIAPPYKRALE 294
           +   F+ CGDLSG+DS++SYPL      P+G      +  Y   D VQPP  P YK AL+
Sbjct: 249 IIDGFVTCGDLSGWDSEKSYPLDIDACFPKGEIDEEQKKRYEFKDVVQPPTDPAYKAALD 290

[Close]

BLAST of MS016715 vs. ExPASy Swiss-Prot
Match: Q54VA8 (Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Dictyostelium discoideum OX=44689 GN=fsjA PE=3 SV=1)

HSP 1 Score: 283.9 bits (725), Expect = 2.1e-75
Identity = 146/268 (54.48%), Postives = 190/268 (70.90%), Query Frame = 0


Query: 1   QDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVISFGYLFVAI 60
           +DIYYRKAKEEGWRARSAFKL+QIDEE+ IFEGVKRVVDLCAAPGSWSQV         +
Sbjct: 9   RDIYYRKAKEEGWRARSAFKLIQIDEEYQIFEGVKRVVDLCAAPGSWSQV---------L 68

Query: 61  SVLLHRDGDLP--LIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIRHFDGCKADLVVCD 120
           S  ++ DG  P   IVA+DLQ MAP++GV+Q++GDIT   T++ +I HFDG  ADL++ D
Sbjct: 69  SRRIYGDGKDPDVKIVAVDLQEMAPLKGVVQIKGDITKYETSKQIISHFDGSLADLIISD 128

Query: 121 GAPDVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQLKLFFPV 180
           GAPDVTGLHD+D + QSQLIL+ L I TH L+ GG F+AK+F+G D SL+Y Q+KLFF  
Sbjct: 129 GAPDVTGLHDIDFYGQSQLILSALNITTHTLKIGGTFVAKMFKGDDMSLMYSQMKLFFEH 188

Query: 181 VTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLE---KVGSPSGGDDLDCSSGWL 240
           V+F KP SSR SS+E F +C NY PP  +NPK +   LE   K+ + +  +  + ++  +
Sbjct: 189 VSFVKPSSSRESSLENFILCRNYQPPLNYNPKIIDPALENHNKILNNNNNNSNNNNNCNI 248

Query: 241 EGPN--------KVYIPFLACGDLS-GY 255
           +  N         + +PF+ACGDL+ GY
Sbjct: 249 DNENDNEFLKTDSLIVPFIACGDLNLGY 267

[Close]

BLAST of MS016715 vs. ExPASy Swiss-Prot
Match: P38238 (tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TRM7 PE=1 SV=1)

HSP 1 Score: 268.1 bits (684), Expect = 1.2e-70
Identity = 144/300 (48.00%), Postives = 198/300 (66.00%), Query Frame = 0


Query: 1   QDIYYRKAKEEGWRARSAFKLLQIDEEFNIFE--GVKRVVDLCAAPGSWSQVISFGYLFV 60
           +D+YYRKAKE+G+RARSAFKLLQ++++F+  +   +KRVVDLCAAPGSWSQV+S      
Sbjct: 9   RDLYYRKAKEQGYRARSAFKLLQLNDQFHFLDDPNLKRVVDLCAAPGSWSQVLSRKLFDE 68

Query: 61  AISVLLHRDGDLPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIRHFDGCKADLVVCD 120
           + S     D +   IV++DLQPM+PI  V  +Q DIT+ +T   +++ F   KAD V  D
Sbjct: 69  SPS----SDKEDRKIVSVDLQPMSPIPHVTTLQADITHPKTLARILKLFGNEKADFVCSD 128

Query: 121 GAPDVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQLKLFFPV 180
           GAPDVTGLHD+DE+VQ QLI++ L +   +L++GG F+AKIFRG+D  +LY QL   F  
Sbjct: 129 GAPDVTGLHDLDEYVQQQLIMSALQLTACILKKGGTFVAKIFRGRDIDMLYSQLGYLFDK 188

Query: 181 VTFAKPKSSRNSSIEAFAVCENYSPPEGFNPK-DLHRLLEKVGSPSGGDDL---DCSSGW 240
           +  AKP+SSR +S+EAF VC  Y+PP  + PK D++  +++       + L   D  S W
Sbjct: 189 IVCAKPRSSRGTSLEAFIVCLGYNPPSNWTPKLDVNTSVDEFFQGCFLNKLCISDKLSHW 248

Query: 241 LEGPNKVYIPFLACGDLSGYDSDRSY-PLPRGAEGAYRSLDPVQPPIAPPYKRALEMKKA 294
            E    +   F+ACG L  +DSD +Y  LP    G   SLDPVQ P  PPYK+ALE+K++
Sbjct: 249 NEEERNI-AEFMACGSLQSFDSDATYHDLPSSVAGTSSSLDPVQSPTNPPYKKALELKRS 303

[Close]

BLAST of MS016715 vs. ExPASy TrEMBL
Match: A0A6J1CXA3 (Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Momordica charantia OX=3673 GN=LOC111015042 PE=3 SV=1)

HSP 1 Score: 580.1 bits (1494), Expect = 5.3e-162
Identity = 287/303 (94.72%), Postives = 290/303 (95.71%), Query Frame = 0


Query: 1   QDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVISFGYLFVA- 60
           +DIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQV+S      A 
Sbjct: 9   RDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLSRKLYLPAK 68

Query: 61  ---ISVLLHRDGDLPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIRHFDGCKADLVV 120
              +S LLHRDGDLPLIVAIDLQPMAPIEGVIQVQGDITNARTA VVIRHFDGCKADLVV
Sbjct: 69  LSSVSNLLHRDGDLPLIVAIDLQPMAPIEGVIQVQGDITNARTAGVVIRHFDGCKADLVV 128

Query: 121 CDGAPDVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQLKLFF 180
           CDGAPDVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQLKLFF
Sbjct: 129 CDGAPDVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQLKLFF 188

Query: 181 PVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDCSSGWLE 240
           PVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPS GDDLDCSSGWLE
Sbjct: 189 PVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSXGDDLDCSSGWLE 248

Query: 241 GPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGAYRSLDPVQPPIAPPYKRALEMKKASSQ 300
           GPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGAYRSLDPVQPPIAPPYKRALEMKKASSQ
Sbjct: 249 GPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGAYRSLDPVQPPIAPPYKRALEMKKASSQ 308

[Close]

BLAST of MS016715 vs. ExPASy TrEMBL
Match: A0A6J1CWI5 (Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Momordica charantia OX=3673 GN=LOC111015042 PE=3 SV=1)

HSP 1 Score: 573.5 bits (1477), Expect = 5.0e-160
Identity = 282/299 (94.31%), Postives = 286/299 (95.65%), Query Frame = 0


Query: 1   QDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVISFGYLFVAI 60
           +DIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQV+S      A 
Sbjct: 9   RDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLSRKLYLPAK 68

Query: 61  SVLLHRDGDLPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIRHFDGCKADLVVCDGA 120
              + +DGDLPLIVAIDLQPMAPIEGVIQVQGDITNARTA VVIRHFDGCKADLVVCDGA
Sbjct: 69  LSSVSKDGDLPLIVAIDLQPMAPIEGVIQVQGDITNARTAGVVIRHFDGCKADLVVCDGA 128

Query: 121 PDVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQLKLFFPVVT 180
           PDVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQLKLFFPVVT
Sbjct: 129 PDVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQLKLFFPVVT 188

Query: 181 FAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDCSSGWLEGPNK 240
           FAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPS GDDLDCSSGWLEGPNK
Sbjct: 189 FAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSXGDDLDCSSGWLEGPNK 248

Query: 241 VYIPFLACGDLSGYDSDRSYPLPRGAEGAYRSLDPVQPPIAPPYKRALEMKKASSQGGR 300
           VYIPFLACGDLSGYDSDRSYPLPRGAEGAYRSLDPVQPPIAPPYKRALEMKKASSQGGR
Sbjct: 249 VYIPFLACGDLSGYDSDRSYPLPRGAEGAYRSLDPVQPPIAPPYKRALEMKKASSQGGR 307

[Close]

BLAST of MS016715 vs. ExPASy TrEMBL
Match: A0A6J1F1P7 (Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111441398 PE=3 SV=1)

HSP 1 Score: 569.3 bits (1466), Expect = 9.4e-159
Identity = 281/300 (93.67%), Postives = 285/300 (95.00%), Query Frame = 0


Query: 1   QDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVISFGYLFVAI 60
           +DIYYRKAKEEGWRARSAFKLLQID+EFNIFEGVKRVVDLCAAPGSWSQV+S      A 
Sbjct: 9   RDIYYRKAKEEGWRARSAFKLLQIDDEFNIFEGVKRVVDLCAAPGSWSQVLSRKLYLPAK 68

Query: 61  SVLLHRDGDLPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIRHFDGCKADLVVCDGA 120
                +DGD PLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIRHFDGCKADLVVCDGA
Sbjct: 69  LSPDSKDGDHPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIRHFDGCKADLVVCDGA 128

Query: 121 PDVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQLKLFFPVVT 180
           PDVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQLKLFFPVVT
Sbjct: 129 PDVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQLKLFFPVVT 188

Query: 181 FAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDCSSGWLEGPNK 240
           FAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDCSSGWLEGPNK
Sbjct: 189 FAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDCSSGWLEGPNK 248

Query: 241 VYIPFLACGDLSGYDSDRSYPLPRGAEGAYRSLDPVQPPIAPPYKRALEMKKASSQGGRE 300
           VYIPFLACGDLSGYDSDRSYPLPRGAEG YRSLDPVQPPIAPPYKRALEMKKASSQG RE
Sbjct: 249 VYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKKASSQGIRE 308

[Close]

BLAST of MS016715 vs. ExPASy TrEMBL
Match: A0A1S3B6C8 (Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Cucumis melo OX=3656 GN=LOC103486316 PE=3 SV=1)

HSP 1 Score: 565.8 bits (1457), Expect = 1.0e-157
Identity = 282/301 (93.69%), Postives = 288/301 (95.68%), Query Frame = 0


Query: 1   QDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVISFG-YLFVA 60
           +DIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQV+S   YL   
Sbjct: 9   RDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLSRKLYLPAK 68

Query: 61  ISVLLHRDGDLPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIRHFDGCKADLVVCDG 120
           +S  L +DGDLPLIVAIDLQPMAPIEGVI+VQGDITNARTAEVVIRHFDGCKADLVVCDG
Sbjct: 69  LSPDL-KDGDLPLIVAIDLQPMAPIEGVIEVQGDITNARTAEVVIRHFDGCKADLVVCDG 128

Query: 121 APDVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQLKLFFPVV 180
           APDVTGLHDMDEFVQ QLILAGLTIVTH+LREGGKFIAKIFRGKDTSLLY QLKLFFPVV
Sbjct: 129 APDVTGLHDMDEFVQFQLILAGLTIVTHILREGGKFIAKIFRGKDTSLLYSQLKLFFPVV 188

Query: 181 TFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDCSSGWLEGPN 240
           TFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDCSSGWLEGPN
Sbjct: 189 TFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDCSSGWLEGPN 248

Query: 241 KVYIPFLACGDLSGYDSDRSYPLPRGAEGAYRSLDPVQPPIAPPYKRALEMKKASSQGGR 300
           KVYIPFLACGDLSGYDSDRSYPLPRGAEG YRSLDPVQPPIAPPYKRALEMKKASSQG R
Sbjct: 249 KVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKKASSQGIR 308

[Close]

BLAST of MS016715 vs. ExPASy TrEMBL
Match: A0A5A7TQN9 (Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G00950 PE=3 SV=1)

HSP 1 Score: 565.5 bits (1456), Expect = 1.4e-157
Identity = 282/300 (94.00%), Postives = 287/300 (95.67%), Query Frame = 0


Query: 2   DIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVISFG-YLFVAI 61
           DIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQV+S   YL   +
Sbjct: 70  DIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLSRKLYLPAKL 129

Query: 62  SVLLHRDGDLPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIRHFDGCKADLVVCDGA 121
           S  L +DGDLPLIVAIDLQPMAPIEGVI+VQGDITNARTAEVVIRHFDGCKADLVVCDGA
Sbjct: 130 SPDL-KDGDLPLIVAIDLQPMAPIEGVIEVQGDITNARTAEVVIRHFDGCKADLVVCDGA 189

Query: 122 PDVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQLKLFFPVVT 181
           PDVTGLHDMDEFVQ QLILAGLTIVTH+LREGGKFIAKIFRGKDTSLLY QLKLFFPVVT
Sbjct: 190 PDVTGLHDMDEFVQFQLILAGLTIVTHILREGGKFIAKIFRGKDTSLLYSQLKLFFPVVT 249

Query: 182 FAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDCSSGWLEGPNK 241
           FAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDCSSGWLEGPNK
Sbjct: 250 FAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDCSSGWLEGPNK 309

Query: 242 VYIPFLACGDLSGYDSDRSYPLPRGAEGAYRSLDPVQPPIAPPYKRALEMKKASSQGGRE 301
           VYIPFLACGDLSGYDSDRSYPLPRGAEG YRSLDPVQPPIAPPYKRALEMKKASSQG RE
Sbjct: 310 VYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKKASSQGIRE 368

[Close]

BLAST of MS016715 vs. TAIR 10
Match: AT5G01230.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 552.0 bits (1421), Expect = 3.0e-157
Identity = 269/297 (90.57%), Postives = 282/297 (94.95%), Query Frame = 0


Query: 1   QDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVISFGYLFVAI 60
           +DIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQV+S      A 
Sbjct: 9   RDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLSRQLYLPAK 68

Query: 61  SVLLHRDGDLPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIRHFDGCKADLVVCDGA 120
           S    +DGDLPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIRHFDGCKADLVVCDGA
Sbjct: 69  SSAESKDGDLPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIRHFDGCKADLVVCDGA 128

Query: 121 PDVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQLKLFFPVVT 180
           PDVTGLHDMDEFVQSQLILAGLTIVTH+L+EGGKFIAKIFRGKDTSLLYCQLKLFFP VT
Sbjct: 129 PDVTGLHDMDEFVQSQLILAGLTIVTHILKEGGKFIAKIFRGKDTSLLYCQLKLFFPTVT 188

Query: 181 FAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDCSSGWLEGPNK 240
           FAKPKSSRNSSIEAFAVCENYSPPEGFNP+DLHRLLEKVGSPSGG DLDCSSGWLEGPNK
Sbjct: 189 FAKPKSSRNSSIEAFAVCENYSPPEGFNPRDLHRLLEKVGSPSGGSDLDCSSGWLEGPNK 248

Query: 241 VYIPFLACGDLSGYDSDRSYPLPRGAEG-AYRSLDPVQPPIAPPYKRALEMKKASSQ 297
           VYIPFLACGDL+GYDSDRSYPLPR A+G +Y+SLDP+QPPIAPPYKRALE+KKAS+Q
Sbjct: 249 VYIPFLACGDLTGYDSDRSYPLPREADGSSYQSLDPIQPPIAPPYKRALELKKASAQ 305

[Close]

BLAST of MS016715 vs. TAIR 10
Match: AT4G25730.1 (FtsJ-like methyltransferase family protein )

HSP 1 Score: 127.9 bits (320), Expect = 1.4e-29
Identity = 77/230 (33.48%), Postives = 125/230 (54.35%), Query Frame = 0


Query: 2   DIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVISFGYLFVAIS 61
           D YYR AKE G+R+R+++KLLQ+D ++++      V+DLCAAPG W Q            
Sbjct: 11  DKYYRLAKERGFRSRASYKLLQLDAKYSLLHSAHAVLDLCAAPGGWMQ------------ 70

Query: 62  VLLHRDGDLPLIVAIDLQPMAPIEGVIQVQGDIT----NARTAEVVIRHFDGCKA-DLVV 121
           V + +     L++ IDL P+ P+ G + +  DIT     ++  +V+ +H  G  A +LV+
Sbjct: 71  VAVEKVPVGSLVLGIDLVPILPVRGCVTMTQDITRTECKSKIKQVMEQH--GVSAFNLVL 130

Query: 122 CDGAPDVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKD-TSLLYCQLKLF 181
            DG+P+V G    +   Q+ L++  + + T  L   G  + K+FR +D  S+LYC  +LF
Sbjct: 131 HDGSPNVGGAWAQEAMSQNALVIDSVRLATEFLARNGNLVTKVFRSRDYNSVLYCLGRLF 190

Query: 182 FPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNPK--DLHRLLEKVGSPS 224
             V  F KP +SR++S E + V   Y  P   +P+  D   L ++   P+
Sbjct: 191 EKVEVF-KPPASRSASAETYLVGLKYLAPAKIDPRLLDYRHLFKESAEPT 225

[Close]

BLAST of MS016715 vs. TAIR 10
Match: AT5G01230.2 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 105.1 bits (261), Expect = 9.7e-23
Identity = 48/49 (97.96%), Postives = 49/49 (100.00%), Query Frame = 0


Query: 1  QDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQ 50
          +DIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQ
Sbjct: 9  RDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQ 57

[Close]

BLAST of MS016715 vs. TAIR 10
Match: AT5G13830.1 (FtsJ-like methyltransferase family protein )

HSP 1 Score: 72.8 bits (177), Expect = 5.3e-13
Identity = 57/227 (25.11%), Postives = 99/227 (43.61%), Query Frame = 0


Query: 2   DIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVI--SFGYLFVA 61
           D +YR+A+  G+ ARSAFKLLQI +++ + +    V+DL  APG+W QV   S G L   
Sbjct: 8   DFFYREAQRLGYVARSAFKLLQIQKQYKLIKPGSSVLDLGCAPGAWLQVACQSLGPL--- 67

Query: 62  ISVLLHRDGDLPLIVAIDLQ----PMAPIEGVIQVQGDITNARTAEVVIRHFDGCKADLV 121
                 R G   ++V +D++    P      V  +  D+ N    ++           ++
Sbjct: 68  ------RSGG--IVVGMDIKKVKVPPQCDSRVQTIAADVLNFPRQKIRELSPQQLGFSVI 127

Query: 122 VCDGAPDVTGLHDMDEFVQSQLILAGLTIVT---------------------HVLREGGK 181
           + D    V+G+   D  + ++L +  L +                        VLR GG 
Sbjct: 128 LSDMCHSVSGITTRDAALSAELGMRALDLAVGQAAISQSPNDDDGGPNESRPGVLRHGGH 187

Query: 182 FIAKIFRGKDTSLLYCQLKLFFPVVTFAKPKSSRNSSIEAFAVCENY 202
            + K+   +D        K  F   ++ +PK++R SS E + +C+ +
Sbjct: 188 LVIKLLESEDAQDFARICKPIFNKASWLRPKATRPSSREIYLICQGF 223

The following BLAST results are available for this feature:

BLAST of MS016715 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022145642.1	1.1e-161	94.72	putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase isoform X1 [Mo...	[more]
XP_022145643.1	1.0e-159	94.31	putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase isoform X2 [Mo...	[more]
KAG6580486.1	1.9e-158	93.67	putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase, partial [Cucu...	[more]
XP_022934133.1	1.9e-158	93.67	putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase [Cucurbita mos...	[more]
XP_038903231.1	2.5e-158	93.67	putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase [Benincasa his...	[more]

BLAST of MS016715 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9UET6	1.1e-92	57.53	Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Homo sapien...	[more]
O36015	2.1e-83	55.33	Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Schizosacch...	[more]
Q22031	4.0e-82	53.62	Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Caenorhabdi...	[more]
Q54VA8	2.1e-75	54.48	Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Dictyosteli...	[more]
P38238	1.2e-70	48.00	tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Saccharomyces cerevi...	[more]

BLAST of MS016715 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CXA3	5.3e-162	94.72	Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Momordica c...	[more]
A0A6J1CWI5	5.0e-160	94.31	Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Momordica c...	[more]
A0A6J1F1P7	9.4e-159	93.67	Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Cucurbita m...	[more]
A0A1S3B6C8	1.0e-157	93.69	Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Cucumis mel...	[more]
A0A5A7TQN9	1.4e-157	94.00	Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Cucumis mel...	[more]

BLAST of MS016715 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G01230.1	3.0e-157	90.57	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein	[more]
AT4G25730.1	1.4e-29	33.48	FtsJ-like methyltransferase family protein	[more]
AT5G01230.2	9.7e-23	97.96	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein	[more]
AT5G13830.1	5.3e-13	25.11	FtsJ-like methyltransferase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 12..207 e-value: 3.6E-70 score: 237.7
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 265..300
None	No IPR available	PANTHER	PTHR10920:SF25	TRNA (CYTIDINE(32)/GUANOSINE(34)-2'-O)-METHYLTRANSFERASE-RELATED	coord: 1..299
None	No IPR available	PANTHER	PTHR10920	RIBOSOMAL RNA METHYLTRANSFERASE	coord: 1..299
IPR002877	Ribosomal RNA methyltransferase FtsJ domain	PFAM	PF01728	FtsJ	coord: 13..201 e-value: 1.4E-62 score: 210.9
IPR015507	Ribosomal RNA large subunit methyltransferase E	HAMAP	MF_01547	RNA_methyltr_E	coord: 1..202 score: 29.040726
IPR028590	tRNA (cytidine(32)/guanosine(34)-2-O)-methyltransferase, Trm7	HAMAP	MF_03162	RNA_methyltr_E_TRM7	coord: 1..260 score: 30.41828
IPR029063	S-adenosyl-L-methionine-dependent methyltransferase	SUPERFAMILY	53335	S-adenosyl-L-methionine-dependent methyltransferases	coord: 12..201

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS016715.1	MS016715.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0032259	methylation
biological_process	GO:0001510	RNA methylation
biological_process	GO:0008033	tRNA processing
molecular_function	GO:0008168	methyltransferase activity
molecular_function	GO:0008175	tRNA methyltransferase activity