MS016686 (gene) Bitter gourd (TR) v1

Overview


Name	MS016686
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Adenine nucleotide alpha hydrolases-like superfamily protein
Location	scaffold563: 105383 .. 106121 (-)
RNA-Seq Expression	MS016686
Synteny	MS016686

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTGAAGGATCGCAAATTTGGAGTGGCTCTCGATTTCTCCAACAGCAGCAAAAATGCTCTGCGATGGGCCATCGATAACTTGGCCGACAAAGGCGACACTCTCTTCGTCATCTACGTAAACCCCAACTCGCTCGATGAATCTGCCCATCAGCTCTGGGCTGAATCCGGCTCTCGTTCGTCATCCCTTTTCTTTTTTCTTTCTCTGATTTCACAGATTTTTGTAAATCCTGTAAATCTGATCCGATTGGGTTTCTGCAGCTCTGATTCCGTTGTCCGAATTCCGAGAGCCGGATGTATTGAGGAAATACGATGTCAAAATCGACATCGAAGCCCTAGATATTCTCGATACTGGCGCTAGACAGAAAGAGGTACTGATTTTGATCAGCCCTTGATTTTAATCACAGTGGTGTGGTGAGGCTAATTTTGAAATTAAACTGTGATTTTAGATTAATGTTGTGGCGAAGCTGTACTGGGGAGATGCGAGGGAGAAGATTATCGATGCGATCGAAGATCTGAAGCTGGAATCTCTGGTAATGGGAAGCAGAGGACTGAGTACCATCCGAAGGTTTGTCTTCTTTCCTGTTCTACAACGAATCATTTGAAGTTTTTGGGAACTGAAAAATTTTCTGTTTGGTTGATCGGAAAACAGGATTCTGCTTGGGAGTGTCAGCAATTACGTGATTACTCATGCTCCTTGCCCTGTGACAGTCGTCAAGGAAGCAAACTTTCAGAGTCAT

mRNA sequence

ATGGTGAAGGATCGCAAATTTGGAGTGGCTCTCGATTTCTCCAACAGCAGCAAAAATGCTCTGCGATGGGCCATCGATAACTTGGCCGACAAAGGCGACACTCTCTTCGTCATCTACGTAAACCCCAACTCGCTCGATGAATCTGCCCATCAGCTCTGGGCTGAATCCGGCTCTCCTCTGATTCCGTTGTCCGAATTCCGAGAGCCGGATGTATTGAGGAAATACGATGTCAAAATCGACATCGAAGCCCTAGATATTCTCGATACTGGCGCTAGACAGAAAGAGATTAATGTTGTGGCGAAGCTGTACTGGGGAGATGCGAGGGAGAAGATTATCGATGCGATCGAAGATCTGAAGCTGGAATCTCTGGTAATGGGAAGCAGAGGACTGAGTACCATCCGAAGGATTCTGCTTGGGAGTGTCAGCAATTACGTGATTACTCATGCTCCTTGCCCTGTGACAGTCGTCAAGGAAGCAAACTTTCAGAGTCAT

Coding sequence (CDS)

ATGGTGAAGGATCGCAAATTTGGAGTGGCTCTCGATTTCTCCAACAGCAGCAAAAATGCTCTGCGATGGGCCATCGATAACTTGGCCGACAAAGGCGACACTCTCTTCGTCATCTACGTAAACCCCAACTCGCTCGATGAATCTGCCCATCAGCTCTGGGCTGAATCCGGCTCTCCTCTGATTCCGTTGTCCGAATTCCGAGAGCCGGATGTATTGAGGAAATACGATGTCAAAATCGACATCGAAGCCCTAGATATTCTCGATACTGGCGCTAGACAGAAAGAGATTAATGTTGTGGCGAAGCTGTACTGGGGAGATGCGAGGGAGAAGATTATCGATGCGATCGAAGATCTGAAGCTGGAATCTCTGGTAATGGGAAGCAGAGGACTGAGTACCATCCGAAGGATTCTGCTTGGGAGTGTCAGCAATTACGTGATTACTCATGCTCCTTGCCCTGTGACAGTCGTCAAGGAAGCAAACTTTCAGAGTCAT

Protein sequence

MVKDRKFGVALDFSNSSKNALRWAIDNLADKGDTLFVIYVNPNSLDESAHQLWAESGSPLIPLSEFREPDVLRKYDVKIDIEALDILDTGARQKEINVVAKLYWGDAREKIIDAIEDLKLESLVMGSRGLSTIRRILLGSVSNYVITHAPCPVTVVKEANFQSH

Homology

[Close]

BLAST of MS016686 vs. NCBI nr
Match: XP_022145652.1 (universal stress protein PHOS32 [Momordica charantia])

HSP 1 Score: 328.9 bits (842), Expect = 2.4e-86
Identity = 164/164 (100.00%), Postives = 164/164 (100.00%), Query Frame = 0


Query: 1   MVKDRKFGVALDFSNSSKNALRWAIDNLADKGDTLFVIYVNPNSLDESAHQLWAESGSPL 60
           MVKDRKFGVALDFSNSSKNALRWAIDNLADKGDTLFVIYVNPNSLDESAHQLWAESGSPL
Sbjct: 1   MVKDRKFGVALDFSNSSKNALRWAIDNLADKGDTLFVIYVNPNSLDESAHQLWAESGSPL 60

Query: 61  IPLSEFREPDVLRKYDVKIDIEALDILDTGARQKEINVVAKLYWGDAREKIIDAIEDLKL 120
           IPLSEFREPDVLRKYDVKIDIEALDILDTGARQKEINVVAKLYWGDAREKIIDAIEDLKL
Sbjct: 61  IPLSEFREPDVLRKYDVKIDIEALDILDTGARQKEINVVAKLYWGDAREKIIDAIEDLKL 120

Query: 121 ESLVMGSRGLSTIRRILLGSVSNYVITHAPCPVTVVKEANFQSH 165
           ESLVMGSRGLSTIRRILLGSVSNYVITHAPCPVTVVKEANFQSH
Sbjct: 121 ESLVMGSRGLSTIRRILLGSVSNYVITHAPCPVTVVKEANFQSH 164

[Close]

BLAST of MS016686 vs. NCBI nr
Match: XP_038903504.1 (universal stress protein PHOS32 [Benincasa hispida])

HSP 1 Score: 313.5 bits (802), Expect = 1.1e-81
Identity = 153/164 (93.29%), Postives = 160/164 (97.56%), Query Frame = 0


Query: 1   MVKDRKFGVALDFSNSSKNALRWAIDNLADKGDTLFVIYVNPNSLDESAHQLWAESGSPL 60
           MVKDRK GVALDFSNSSKNALRWAIDNLADKGDTLF+IYVNPNSLDESAH+LWAESGSPL
Sbjct: 1   MVKDRKIGVALDFSNSSKNALRWAIDNLADKGDTLFIIYVNPNSLDESAHRLWAESGSPL 60

Query: 61  IPLSEFREPDVLRKYDVKIDIEALDILDTGARQKEINVVAKLYWGDAREKIIDAIEDLKL 120
           IPLSEFREPDVL+KYDVKIDIEALD LDTGARQKEI VV+KLYWGDAREKI+DAIEDLKL
Sbjct: 61  IPLSEFREPDVLKKYDVKIDIEALDTLDTGARQKEITVVSKLYWGDAREKIVDAIEDLKL 120

Query: 121 ESLVMGSRGLSTIRRILLGSVSNYVITHAPCPVTVVKEANFQSH 165
           +SLVMGSRGLSTIRRILLGSVSNYVITHAPCPVTVVKE+NFQ H
Sbjct: 121 DSLVMGSRGLSTIRRILLGSVSNYVITHAPCPVTVVKESNFQHH 164

[Close]

BLAST of MS016686 vs. NCBI nr
Match: XP_011651876.1 (universal stress protein PHOS32 [Cucumis sativus] >KGN58822.1 hypothetical protein Csa_001140 [Cucumis sativus])

HSP 1 Score: 308.9 bits (790), Expect = 2.6e-80
Identity = 150/164 (91.46%), Postives = 160/164 (97.56%), Query Frame = 0


Query: 1   MVKDRKFGVALDFSNSSKNALRWAIDNLADKGDTLFVIYVNPNSLDESAHQLWAESGSPL 60
           MVKDRK GVALDFSNSSKNALRWAIDNLADKGDTLF+IYVNPNSL+ESAH+LWAESGSPL
Sbjct: 1   MVKDRKIGVALDFSNSSKNALRWAIDNLADKGDTLFIIYVNPNSLEESAHRLWAESGSPL 60

Query: 61  IPLSEFREPDVLRKYDVKIDIEALDILDTGARQKEINVVAKLYWGDAREKIIDAIEDLKL 120
           IPLSEFREP+VL+KYDVKIDIEALDILDTGARQKEI VV+KLYWGDAREKI+DAIEDLKL
Sbjct: 61  IPLSEFREPEVLKKYDVKIDIEALDILDTGARQKEITVVSKLYWGDAREKIVDAIEDLKL 120

Query: 121 ESLVMGSRGLSTIRRILLGSVSNYVITHAPCPVTVVKEANFQSH 165
           +SLVMGSRGLSTIRRILLGSVSNYVITHAPCPVTVVK++NF  H
Sbjct: 121 DSLVMGSRGLSTIRRILLGSVSNYVITHAPCPVTVVKDSNFPHH 164

[Close]

BLAST of MS016686 vs. NCBI nr
Match: XP_008442491.1 (PREDICTED: uncharacterized protein C167.05 [Cucumis melo] >KAA0044129.1 universal stress protein PHOS32-like [Cucumis melo var. makuwa] >TYK25009.1 universal stress protein PHOS32-like [Cucumis melo var. makuwa])

HSP 1 Score: 303.1 bits (775), Expect = 1.4e-78
Identity = 146/164 (89.02%), Postives = 160/164 (97.56%), Query Frame = 0


Query: 1   MVKDRKFGVALDFSNSSKNALRWAIDNLADKGDTLFVIYVNPNSLDESAHQLWAESGSPL 60
           MVKDRK GVALDFS+SSKNAL+WAIDNLADKGDTL++IYVNPNSL+ESAH+LWAESGSPL
Sbjct: 1   MVKDRKIGVALDFSDSSKNALKWAIDNLADKGDTLYIIYVNPNSLNESAHRLWAESGSPL 60

Query: 61  IPLSEFREPDVLRKYDVKIDIEALDILDTGARQKEINVVAKLYWGDAREKIIDAIEDLKL 120
           IPLSEFREP+VL+KYDVKIDI+ALDILDTGARQKEI VV+KLYWGDAREKI+DAIEDLKL
Sbjct: 61  IPLSEFREPEVLKKYDVKIDIDALDILDTGARQKEITVVSKLYWGDAREKIVDAIEDLKL 120

Query: 121 ESLVMGSRGLSTIRRILLGSVSNYVITHAPCPVTVVKEANFQSH 165
           +SLVMGSRGLSTIRRILLGSVSNYVITHAPCPVTVVK++NF  H
Sbjct: 121 DSLVMGSRGLSTIRRILLGSVSNYVITHAPCPVTVVKDSNFPHH 164

[Close]

BLAST of MS016686 vs. NCBI nr
Match: XP_022935543.1 (universal stress protein PHOS32-like [Cucurbita moschata])

HSP 1 Score: 295.4 bits (755), Expect = 3.0e-76
Identity = 143/162 (88.27%), Postives = 155/162 (95.68%), Query Frame = 0


Query: 1   MVKDRKFGVALDFSNSSKNALRWAIDNLADKGDTLFVIYVNPNSLDESAHQLWAESGSPL 60
           MVKDRK GVALDFS+SSKNALRWAIDNLADKGDTLF+IYVNPNS++ES H+LWAESGSPL
Sbjct: 50  MVKDRKIGVALDFSSSSKNALRWAIDNLADKGDTLFIIYVNPNSVNESPHKLWAESGSPL 109

Query: 61  IPLSEFREPDVLRKYDVKIDIEALDILDTGARQKEINVVAKLYWGDAREKIIDAIEDLKL 120
           IPLSEFREPDVL+ YDV++DIEALDILDTG+RQKEI VVAKLYWGDAREKI+DAIEDLKL
Sbjct: 110 IPLSEFREPDVLKSYDVEVDIEALDILDTGSRQKEIQVVAKLYWGDAREKIVDAIEDLKL 169

Query: 121 ESLVMGSRGLSTIRRILLGSVSNYVITHAPCPVTVVKEANFQ 163
           E LVMGSRG STIRRILLGSVSNYVITHAPCPVTVVK++ FQ
Sbjct: 170 ECLVMGSRGQSTIRRILLGSVSNYVITHAPCPVTVVKDSKFQ 211

[Close]

BLAST of MS016686 vs. ExPASy Swiss-Prot
Match: Q8VYN9 (Universal stress protein PHOS32 OS=Arabidopsis thaliana OX=3702 GN=PHOS32 PE=1 SV=1)

HSP 1 Score: 77.0 bits (188), Expect = 2.2e-13
Identity = 52/163 (31.90%), Postives = 84/163 (51.53%), Query Frame = 0


Query: 5   RKFGVALDFSNSSKNALRWAIDNLADKGDTLFVIYVNPNSLDESAHQLWAESGSPLIPLS 64
           RK GVA+D S  S  A+RWA+D+    GD + +++V+P S+      L+     PL   +
Sbjct: 48  RKIGVAVDLSEESSFAVRWAVDHYIRPGDAVVLLHVSPTSV------LFGADWGPLPLKT 107

Query: 65  EFREPDVLRKYDVKIDIEAL---DILDTGARQKEINVVAKLYW---GDAREKIIDAIEDL 124
           +  +P+  +    + D +A     + D     KE+    K++     D RE++   IE L
Sbjct: 108 QIEDPNA-QPQPSQEDFDAFTSTKVADLAKPLKELGFPYKIHIVKDHDMRERLCLEIERL 167

Query: 125 KLESLVMGSRGLSTIRRI----LLGSVSNYVITHAPCPVTVVK 158
            L +++MGSRG    ++      LGSVS+Y + H  CPV VV+
Sbjct: 168 GLSAVIMGSRGFGAEKKRGSDGKLGSVSDYCVHHCVCPVVVVR 203

[Close]

BLAST of MS016686 vs. ExPASy Swiss-Prot
Match: Q8L4N1 (Universal stress protein PHOS34 OS=Arabidopsis thaliana OX=3702 GN=PHOS34 PE=1 SV=1)

HSP 1 Score: 73.2 bits (178), Expect = 3.1e-12
Identity = 55/167 (32.93%), Postives = 81/167 (48.50%), Query Frame = 0


Query: 5   RKFGVALDFSNSSKNALRWAIDNLADKGDTLFVIYVNPNSLDESAHQLWAESGSPL---- 64
           RK GVA+D S  S  A+RWA+D+    GD + +++V+P S+      L+     PL    
Sbjct: 45  RKIGVAVDLSEESAFAVRWAVDHYIRPGDAVVILHVSPTSV------LFGADWGPLPLQT 104

Query: 65  -IPLSEFREPDVLRKYDVKIDIEAL---DILDTGARQKEINVVAKLYW---GDAREKIID 124
             P S   +P    K   + D +A     + D     KE     K++     D RE++  
Sbjct: 105 PPPPSAATDPGAQPKPSQE-DFDAFTSSKVADLAKPLKEAGFPHKIHIVKDHDMRERLCL 164

Query: 125 AIEDLKLESLVMGSRGLSTIRR---ILLGSVSNYVITHAPCPVTVVK 158
             E L L +++MGSRG    +R     LGSVS+Y + H  CPV VV+
Sbjct: 165 ETERLNLSAVIMGSRGFGAEKRGSDGKLGSVSDYCVHHCVCPVVVVR 204

[Close]

BLAST of MS016686 vs. ExPASy Swiss-Prot
Match: P87132 (Uncharacterized protein C167.05 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC167.05 PE=1 SV=2)

HSP 1 Score: 61.6 bits (148), Expect = 9.4e-09
Identity = 45/150 (30.00%), Postives = 73/150 (48.67%), Query Frame = 0


Query: 9   VALDFSNSSKNALRWAIDNLADKGDTLFVIYVNPNSLDESAHQLWAESGSPLIPLSEFRE 68
           + LD S+ S +A  WA+  L   GDTL ++ V     D SA  +     S  +   E   
Sbjct: 435 LTLDLSSESLHAAEWAVGILLRNGDTLIIVDVIECD-DPSARAVKDRMESEQLETLE--- 494

Query: 69  PDVLRKYDVKIDIEALDILDTGARQKEINVVAKLYWGDAREKIIDAIEDLKLESLVMGSR 128
                    KI    L +L     + E+N+   ++   A+  II+ I+ ++   +VMGSR
Sbjct: 495 ---------KITKYILKLLSKTVLEVEVNIEV-IHHEKAKHLIIEMIDYIEPSLVVMGSR 554

Query: 129 GLSTIRRILLGSVSNYVITHAPCPVTVVKE 159
           G S ++ +LLGS SNY++  +  PV V ++
Sbjct: 555 GRSHLKGVLLGSFSNYLVNKSSVPVMVARK 570

[Close]

BLAST of MS016686 vs. ExPASy Swiss-Prot
Match: Q8LGG8 (Universal stress protein A-like protein OS=Arabidopsis thaliana OX=3702 GN=At3g01520 PE=1 SV=2)

HSP 1 Score: 53.5 bits (127), Expect = 2.6e-06
Identity = 33/145 (22.76%), Postives = 70/145 (48.28%), Query Frame = 0


Query: 14  SNSSKNALRWAIDNLADKGDTLF-VIYVNPNSLDESAHQLWAESGSPLIPLSEFREPDVL 73
           S S K A  W ++ +     + F ++ ++   +DE     + +  S      +FR+   +
Sbjct: 24  SISCKRAFEWTLEKIVRSNTSDFKILLLHVQVVDEDG---FDDVDSIYASPEDFRD---M 83

Query: 74  RKYDVKIDIEALDILDTGARQKEINVVAKLYWGDAREKIIDAIEDLKLESLVMGSRGLST 133
           R+ +    +  L+       +  +   A +  GD ++ I   ++ ++ + LV+GSRGL  
Sbjct: 84  RQSNKAKGLHLLEFFVNKCHEIGVGCEAWIKTGDPKDVICQEVKRVRPDFLVVGSRGLGR 143

Query: 134 IRRILLGSVSNYVITHAPCPVTVVK 158
            +++ +G+VS + + HA CPV  +K
Sbjct: 144 FQKVFVGTVSAFCVKHAECPVMTIK 162

[Close]

BLAST of MS016686 vs. ExPASy Swiss-Prot
Match: Q57951 (Universal stress protein MJ0531 OS=Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) OX=243232 GN=MJ0531 PE=3 SV=1)

HSP 1 Score: 51.6 bits (122), Expect = 9.8e-06
Identity = 32/113 (28.32%), Postives = 58/113 (51.33%), Query Frame = 0


Query: 46  DESAHQLWAESGSPLIPLSEFREPDVLRKYDVKIDIEALDILDTGARQKEINVVAKLYWG 105
           D   + ++    SP + L      +++ +   +   EAL  +   A +  + +  ++  G
Sbjct: 54  DAEVYAIYVVDVSPFVGLPAEGSWELISELLKEEGQEALKKVKKMAEEWGVKIHTEMLEG 113

Query: 106 DAREKIIDAIEDLKLESLVMGSRGLSTIRRILLGSVSNYVITHAPCPVTVVKE 159
               +I++  E  K + +VMG+ G + + RILLGSV+  VI +A CPV VVK+
Sbjct: 114 VPANEIVEFAEKKKADLIVMGTTGKTGLERILLGSVAERVIKNAHCPVLVVKK 166

[Close]

BLAST of MS016686 vs. ExPASy TrEMBL
Match: A0A6J1CXB3 (universal stress protein PHOS32 OS=Momordica charantia OX=3673 GN=LOC111015049 PE=4 SV=1)

HSP 1 Score: 328.9 bits (842), Expect = 1.2e-86
Identity = 164/164 (100.00%), Postives = 164/164 (100.00%), Query Frame = 0


Query: 1   MVKDRKFGVALDFSNSSKNALRWAIDNLADKGDTLFVIYVNPNSLDESAHQLWAESGSPL 60
           MVKDRKFGVALDFSNSSKNALRWAIDNLADKGDTLFVIYVNPNSLDESAHQLWAESGSPL
Sbjct: 1   MVKDRKFGVALDFSNSSKNALRWAIDNLADKGDTLFVIYVNPNSLDESAHQLWAESGSPL 60

Query: 61  IPLSEFREPDVLRKYDVKIDIEALDILDTGARQKEINVVAKLYWGDAREKIIDAIEDLKL 120
           IPLSEFREPDVLRKYDVKIDIEALDILDTGARQKEINVVAKLYWGDAREKIIDAIEDLKL
Sbjct: 61  IPLSEFREPDVLRKYDVKIDIEALDILDTGARQKEINVVAKLYWGDAREKIIDAIEDLKL 120

Query: 121 ESLVMGSRGLSTIRRILLGSVSNYVITHAPCPVTVVKEANFQSH 165
           ESLVMGSRGLSTIRRILLGSVSNYVITHAPCPVTVVKEANFQSH
Sbjct: 121 ESLVMGSRGLSTIRRILLGSVSNYVITHAPCPVTVVKEANFQSH 164

[Close]

BLAST of MS016686 vs. ExPASy TrEMBL
Match: A0A0A0LDK3 (Usp domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G733270 PE=4 SV=1)

HSP 1 Score: 308.9 bits (790), Expect = 1.3e-80
Identity = 150/164 (91.46%), Postives = 160/164 (97.56%), Query Frame = 0


Query: 1   MVKDRKFGVALDFSNSSKNALRWAIDNLADKGDTLFVIYVNPNSLDESAHQLWAESGSPL 60
           MVKDRK GVALDFSNSSKNALRWAIDNLADKGDTLF+IYVNPNSL+ESAH+LWAESGSPL
Sbjct: 1   MVKDRKIGVALDFSNSSKNALRWAIDNLADKGDTLFIIYVNPNSLEESAHRLWAESGSPL 60

Query: 61  IPLSEFREPDVLRKYDVKIDIEALDILDTGARQKEINVVAKLYWGDAREKIIDAIEDLKL 120
           IPLSEFREP+VL+KYDVKIDIEALDILDTGARQKEI VV+KLYWGDAREKI+DAIEDLKL
Sbjct: 61  IPLSEFREPEVLKKYDVKIDIEALDILDTGARQKEITVVSKLYWGDAREKIVDAIEDLKL 120

Query: 121 ESLVMGSRGLSTIRRILLGSVSNYVITHAPCPVTVVKEANFQSH 165
           +SLVMGSRGLSTIRRILLGSVSNYVITHAPCPVTVVK++NF  H
Sbjct: 121 DSLVMGSRGLSTIRRILLGSVSNYVITHAPCPVTVVKDSNFPHH 164

[Close]

BLAST of MS016686 vs. ExPASy TrEMBL
Match: A0A5A7TSD0 (Universal stress protein PHOS32-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G001270 PE=4 SV=1)

HSP 1 Score: 303.1 bits (775), Expect = 6.9e-79
Identity = 146/164 (89.02%), Postives = 160/164 (97.56%), Query Frame = 0


Query: 1   MVKDRKFGVALDFSNSSKNALRWAIDNLADKGDTLFVIYVNPNSLDESAHQLWAESGSPL 60
           MVKDRK GVALDFS+SSKNAL+WAIDNLADKGDTL++IYVNPNSL+ESAH+LWAESGSPL
Sbjct: 1   MVKDRKIGVALDFSDSSKNALKWAIDNLADKGDTLYIIYVNPNSLNESAHRLWAESGSPL 60

Query: 61  IPLSEFREPDVLRKYDVKIDIEALDILDTGARQKEINVVAKLYWGDAREKIIDAIEDLKL 120
           IPLSEFREP+VL+KYDVKIDI+ALDILDTGARQKEI VV+KLYWGDAREKI+DAIEDLKL
Sbjct: 61  IPLSEFREPEVLKKYDVKIDIDALDILDTGARQKEITVVSKLYWGDAREKIVDAIEDLKL 120

Query: 121 ESLVMGSRGLSTIRRILLGSVSNYVITHAPCPVTVVKEANFQSH 165
           +SLVMGSRGLSTIRRILLGSVSNYVITHAPCPVTVVK++NF  H
Sbjct: 121 DSLVMGSRGLSTIRRILLGSVSNYVITHAPCPVTVVKDSNFPHH 164

[Close]

BLAST of MS016686 vs. ExPASy TrEMBL
Match: A0A1S3B5T0 (uncharacterized protein C167.05 OS=Cucumis melo OX=3656 GN=LOC103486344 PE=4 SV=1)

HSP 1 Score: 303.1 bits (775), Expect = 6.9e-79
Identity = 146/164 (89.02%), Postives = 160/164 (97.56%), Query Frame = 0


Query: 1   MVKDRKFGVALDFSNSSKNALRWAIDNLADKGDTLFVIYVNPNSLDESAHQLWAESGSPL 60
           MVKDRK GVALDFS+SSKNAL+WAIDNLADKGDTL++IYVNPNSL+ESAH+LWAESGSPL
Sbjct: 1   MVKDRKIGVALDFSDSSKNALKWAIDNLADKGDTLYIIYVNPNSLNESAHRLWAESGSPL 60

Query: 61  IPLSEFREPDVLRKYDVKIDIEALDILDTGARQKEINVVAKLYWGDAREKIIDAIEDLKL 120
           IPLSEFREP+VL+KYDVKIDI+ALDILDTGARQKEI VV+KLYWGDAREKI+DAIEDLKL
Sbjct: 61  IPLSEFREPEVLKKYDVKIDIDALDILDTGARQKEITVVSKLYWGDAREKIVDAIEDLKL 120

Query: 121 ESLVMGSRGLSTIRRILLGSVSNYVITHAPCPVTVVKEANFQSH 165
           +SLVMGSRGLSTIRRILLGSVSNYVITHAPCPVTVVK++NF  H
Sbjct: 121 DSLVMGSRGLSTIRRILLGSVSNYVITHAPCPVTVVKDSNFPHH 164

[Close]

BLAST of MS016686 vs. ExPASy TrEMBL
Match: A0A6J1FAU1 (universal stress protein PHOS32-like OS=Cucurbita moschata OX=3662 GN=LOC111442383 PE=4 SV=1)

HSP 1 Score: 295.4 bits (755), Expect = 1.4e-76
Identity = 143/162 (88.27%), Postives = 155/162 (95.68%), Query Frame = 0


Query: 1   MVKDRKFGVALDFSNSSKNALRWAIDNLADKGDTLFVIYVNPNSLDESAHQLWAESGSPL 60
           MVKDRK GVALDFS+SSKNALRWAIDNLADKGDTLF+IYVNPNS++ES H+LWAESGSPL
Sbjct: 50  MVKDRKIGVALDFSSSSKNALRWAIDNLADKGDTLFIIYVNPNSVNESPHKLWAESGSPL 109

Query: 61  IPLSEFREPDVLRKYDVKIDIEALDILDTGARQKEINVVAKLYWGDAREKIIDAIEDLKL 120
           IPLSEFREPDVL+ YDV++DIEALDILDTG+RQKEI VVAKLYWGDAREKI+DAIEDLKL
Sbjct: 110 IPLSEFREPDVLKSYDVEVDIEALDILDTGSRQKEIQVVAKLYWGDAREKIVDAIEDLKL 169

Query: 121 ESLVMGSRGLSTIRRILLGSVSNYVITHAPCPVTVVKEANFQ 163
           E LVMGSRG STIRRILLGSVSNYVITHAPCPVTVVK++ FQ
Sbjct: 170 ECLVMGSRGQSTIRRILLGSVSNYVITHAPCPVTVVKDSKFQ 211

[Close]

BLAST of MS016686 vs. TAIR 10
Match: AT3G53990.1 (Adenine nucleotide alpha hydrolases-like superfamily protein )

HSP 1 Score: 238.8 bits (608), Expect = 3.1e-63
Identity = 108/158 (68.35%), Postives = 139/158 (87.97%), Query Frame = 0


Query: 1   MVKDRKFGVALDFSNSSKNALRWAIDNLADKGDTLFVIYVNPNSLDESAHQLWAESGSPL 60
           M KDR  G+A+DFS SSKNAL+WAI+NLADKGDT+++I+  P S DES + LW +SGSPL
Sbjct: 1   MPKDRNIGIAMDFSESSKNALKWAIENLADKGDTIYIIHTLPLSGDESRNSLWFKSGSPL 60

Query: 61  IPLSEFREPDVLRKYDVKIDIEALDILDTGARQKEINVVAKLYWGDAREKIIDAIEDLKL 120
           IPL+EFREP+++ KY VK DI  LD+LDTG+RQKE++VV KLYWGDAREK++DA++DLKL
Sbjct: 61  IPLAEFREPEIMEKYGVKTDIACLDMLDTGSRQKEVHVVTKLYWGDAREKLVDAVKDLKL 120

Query: 121 ESLVMGSRGLSTIRRILLGSVSNYVITHAPCPVTVVKE 159
           +S+VMGSRGLS ++RI++GSVS++VI HAPCPVTVVK+
Sbjct: 121 DSIVMGSRGLSALQRIIMGSVSSFVIQHAPCPVTVVKD 158

[Close]

BLAST of MS016686 vs. TAIR 10
Match: AT3G03270.2 (Adenine nucleotide alpha hydrolases-like superfamily protein )

HSP 1 Score: 188.0 bits (476), Expect = 6.2e-48
Identity = 89/157 (56.69%), Postives = 123/157 (78.34%), Query Frame = 0


Query: 1   MVKDRKFGVALDFSNSSKNALRWAIDNLADKGDTLFVIYVNPNSLDESAHQLWAESGSPL 60
           M K R  GV +D+S +SK ALRWA +NL + GDT+ +I+V P + D +   L+ E+GSPL
Sbjct: 1   MGKARTVGVGMDYSPTSKLALRWAAENLLEDGDTVILIHVQPQNADHTRKILFEETGSPL 60

Query: 61  IPLSEFREPDVLRKYDVKIDIEALDILDTGARQKEINVVAKLYWGDAREKIIDAIEDLKL 120
           IPL EFRE ++ ++Y +  D E LD+LDT +R K++ VVAK+YWGD REK+ DA+E+LKL
Sbjct: 61  IPLEEFREVNLSKQYGLAYDPEVLDVLDTLSRAKKVKVVAKVYWGDPREKLCDAVENLKL 120

Query: 121 ESLVMGSRGLSTIRRILLGSVSNYVITHAPCPVTVVK 158
           +S+V+GSRGL +++RILLGSVSN+V+T+A CPVTVVK
Sbjct: 121 DSIVLGSRGLGSLKRILLGSVSNHVVTNATCPVTVVK 157

[Close]

BLAST of MS016686 vs. TAIR 10
Match: AT3G17020.1 (Adenine nucleotide alpha hydrolases-like superfamily protein )

HSP 1 Score: 167.2 bits (422), Expect = 1.1e-41
Identity = 82/154 (53.25%), Postives = 110/154 (71.43%), Query Frame = 0


Query: 5   RKFGVALDFSNSSKNALRWAIDNLADKGDTLFVIYV-NPNSLDESAHQLWAESGSPLIPL 64
           R+ GVA+DFS+ SK AL WAIDN+   GD L +I + +  + +E   QLW   GSP IP+
Sbjct: 7   RRIGVAVDFSDCSKKALSWAIDNVVRDGDHLILITIAHDMNYEEGEMQLWETVGSPFIPM 66

Query: 65  SEFREPDVLRKYDVKIDIEALDILDTGARQKEINVVAKLYWGDAREKIIDAIEDLKLESL 124
           SEF +  V++KY +K D E LDI++T AR+K I VV K+YWGD REKI  A E + L SL
Sbjct: 67  SEFSDAAVMKKYALKPDAETLDIVNTAARKKTITVVMKIYWGDPREKICAAAEQIPLSSL 126

Query: 125 VMGSRGLSTIRRILLGSVSNYVITHAPCPVTVVK 158
           VMG+RGL  ++R+++GSVSN+V+ +  CPVTVVK
Sbjct: 127 VMGNRGLGGLKRMIMGSVSNHVVNNVACPVTVVK 160

[Close]

BLAST of MS016686 vs. TAIR 10
Match: AT3G03270.1 (Adenine nucleotide alpha hydrolases-like superfamily protein )

HSP 1 Score: 153.7 bits (387), Expect = 1.3e-37
Identity = 71/135 (52.59%), Postives = 102/135 (75.56%), Query Frame = 0


Query: 1   MVKDRKFGVALDFSNSSKNALRWAIDNLADKGDTLFVIYVNPNSLDESAHQLWAESGSPL 60
           M K R  GV +D+S +SK ALRWA +NL + GDT+ +I+V P + D +   L+ E+GSPL
Sbjct: 1   MGKARTVGVGMDYSPTSKLALRWAAENLLEDGDTVILIHVQPQNADHTRKILFEETGSPL 60

Query: 61  IPLSEFREPDVLRKYDVKIDIEALDILDTGARQKEINVVAKLYWGDAREKIIDAIEDLKL 120
           IPL EFRE ++ ++Y +  D E LD+LDT +R K++ VVAK+YWGD REK+ DA+E+LKL
Sbjct: 61  IPLEEFREVNLSKQYGLAYDPEVLDVLDTLSRAKKVKVVAKVYWGDPREKLCDAVENLKL 120

Query: 121 ESLVMGSRGLSTIRR 136
           +S+V+GSRGL +++R
Sbjct: 121 DSIVLGSRGLGSLKR 135

[Close]

BLAST of MS016686 vs. TAIR 10
Match: AT3G53990.2 (Adenine nucleotide alpha hydrolases-like superfamily protein )

HSP 1 Score: 140.2 bits (352), Expect = 1.5e-33
Identity = 63/96 (65.62%), Postives = 80/96 (83.33%), Query Frame = 0


Query: 1  MVKDRKFGVALDFSNSSKNALRWAIDNLADKGDTLFVIYVNPNSLDESAHQLWAESGSPL 60
          M KDR  G+A+DFS SSKNAL+WAI+NLADKGDT+++I+  P S DES + LW +SGSPL
Sbjct: 1  MPKDRNIGIAMDFSESSKNALKWAIENLADKGDTIYIIHTLPLSGDESRNSLWFKSGSPL 60

Query: 61 IPLSEFREPDVLRKYDVKIDIEALDILDTGARQKEI 97
          IPL+EFREP+++ KY VK DI  LD+LDTG+RQKE+
Sbjct: 61 IPLAEFREPEIMEKYGVKTDIACLDMLDTGSRQKEV 96

The following BLAST results are available for this feature:

BLAST of MS016686 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022145652.1	2.4e-86	100.00	universal stress protein PHOS32 [Momordica charantia]	[more]
XP_038903504.1	1.1e-81	93.29	universal stress protein PHOS32 [Benincasa hispida]	[more]
XP_011651876.1	2.6e-80	91.46	universal stress protein PHOS32 [Cucumis sativus] >KGN58822.1 hypothetical prote...	[more]
XP_008442491.1	1.4e-78	89.02	PREDICTED: uncharacterized protein C167.05 [Cucumis melo] >KAA0044129.1 universa...	[more]
XP_022935543.1	3.0e-76	88.27	universal stress protein PHOS32-like [Cucurbita moschata]	[more]

BLAST of MS016686 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8VYN9	2.2e-13	31.90	Universal stress protein PHOS32 OS=Arabidopsis thaliana OX=3702 GN=PHOS32 PE=1 S...	[more]
Q8L4N1	3.1e-12	32.93	Universal stress protein PHOS34 OS=Arabidopsis thaliana OX=3702 GN=PHOS34 PE=1 S...	[more]
P87132	9.4e-09	30.00	Uncharacterized protein C167.05 OS=Schizosaccharomyces pombe (strain 972 / ATCC ...	[more]
Q8LGG8	2.6e-06	22.76	Universal stress protein A-like protein OS=Arabidopsis thaliana OX=3702 GN=At3g0...	[more]
Q57951	9.8e-06	28.32	Universal stress protein MJ0531 OS=Methanocaldococcus jannaschii (strain ATCC 43...	[more]

BLAST of MS016686 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CXB3	1.2e-86	100.00	universal stress protein PHOS32 OS=Momordica charantia OX=3673 GN=LOC111015049 P...	[more]
A0A0A0LDK3	1.3e-80	91.46	Usp domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G733270 PE=4 SV...	[more]
A0A5A7TSD0	6.9e-79	89.02	Universal stress protein PHOS32-like OS=Cucumis melo var. makuwa OX=1194695 GN=E...	[more]
A0A1S3B5T0	6.9e-79	89.02	uncharacterized protein C167.05 OS=Cucumis melo OX=3656 GN=LOC103486344 PE=4 SV=...	[more]
A0A6J1FAU1	1.4e-76	88.27	universal stress protein PHOS32-like OS=Cucurbita moschata OX=3662 GN=LOC1114423...	[more]

BLAST of MS016686 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G53990.1	3.1e-63	68.35	Adenine nucleotide alpha hydrolases-like superfamily protein	[more]
AT3G03270.2	6.2e-48	56.69	Adenine nucleotide alpha hydrolases-like superfamily protein	[more]
AT3G17020.1	1.1e-41	53.25	Adenine nucleotide alpha hydrolases-like superfamily protein	[more]
AT3G03270.1	1.3e-37	52.59	Adenine nucleotide alpha hydrolases-like superfamily protein	[more]
AT3G53990.2	1.5e-33	65.63	Adenine nucleotide alpha hydrolases-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006015	Universal stress protein A family	PRINTS	PR01438	UNVRSLSTRESS	coord: 117..129 score: 33.23 coord: 4..22 score: 28.63 coord: 135..157 score: 46.78
IPR006016	UspA	PFAM	PF00582	Usp	coord: 5..157 e-value: 8.7E-34 score: 117.1
IPR014729	Rossmann-like alpha/beta/alpha sandwich fold	GENE3D	3.40.50.620	HUPs	coord: 2..163 e-value: 3.2E-36 score: 127.2
None	No IPR available	PANTHER	PTHR46100	IMP2'P	coord: 4..163
None	No IPR available	PANTHER	PTHR46100:SF2	OS05G0453700 PROTEIN	coord: 4..163
None	No IPR available	CDD	cd00293	USP_Like	coord: 9..156 e-value: 4.70478E-29 score: 102.063
None	No IPR available	SUPERFAMILY	52402	Adenine nucleotide alpha hydrolases-like	coord: 5..157

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS016686.1	MS016686.1	mRNA