MS016496 (gene) Bitter gourd (TR) v1

Overview
NameMS016496
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
Descriptionphotosystem I assembly factor PSA3, chloroplastic
Locationscaffold657: 155998 .. 158515 (+)
RNA-Seq ExpressionMS016496
SyntenyMS016496
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCCCTGTCCTCTACTTCCACTTCATCTTCTTTCCGCCCAAATCTCAATTCCATTTCCATTCCCAATCCCAGAACCTGCTTCCTCTGTAATCCGATTCCGACTTCTCGGGCGATTCTCCTCCTCCGCCGCCGCCGAGCCTTGTCCGTGAGATCGTACATGGAGAATCCGAATTCTCTGTCCGGATTCGCGAACAAGGTCATCGGTTCTCTCCCGGTGGTCGGCCTGATCGCCAGGATTCTCAGCGACGAAGGCGGCGTCGGCACCGACATTATCGATTTCGCCGAGTTTCGGAGACGAGTTGGGAAGAAATGTACGGTCATGGATTCTAGGGCTTTCTACGAATTTCAAAACAGAAGAGGCAAGGTTAGTGTGTAACTGTATATATATATGTAGAGATTTTTAATTAATTACTTTCTTTAGCAAATCACTTTTTTTTTTAAATGTTATGGTGTTTAATTGAAACCTCTTCTTTCTACTGTGGAAGAATTATTAATTTATTTGGAGTTTAGAGTGTATTTATTATAGTTTTTTCTAAGTGGTTTTAAATAGTTAACACTGATTGGATAAAAATTTTCTATATTAGTTTTTTTCAATACTATTTTAATATTTGGTTTCTATCATTTTTGCGGACTATTTAAAATTATTTCTCAAACTTTAAGAATTAAAAGGTGTTCGTTTTGAAATTTCATGGACCATGTAAACATCGAATTCAAGCTCGGGTCAAAAGTCTACATTTCACTATTTCAGACCAAATAGACAGTATCTATATTTTTATGGTGAGATTGATATCTTGATTGATCCCAATTTTTAAAATTTGAGTCAAAAAAATCAGCTACACAAGCCCTCCTGTCAGAAACAAAACACTAAGGCAAACCCTTTTCTAAATAGAGTTAATGGTGGAAGTTAAAAAGATTTAAAATCAAATCTTATGAATTCTTAAATTTATTATTATTATTTATTTTAATACAGGTACTTTTTTCGTTTTGGTCCTATGCTTAAAAAGAAAATATCCTTTTGTCTCTTTATTTTATTTTTTATCTCATTTTTTAGTCACAATTTCAACGAGACACTACTTTTAATATATTTCTTAAAATGTATTGATTTCTAGCTTTTTCGTTGAATCTGTGTTAAAATTTATGATTAAAATGAAAAGTCGAATGTATAAGGTTTAAAATGGAACCAAAATAAATGTTTTCAAAATATTTTGATCAAAATGAATCAAAGCTGAATATATCAGCACCAAAATAATATGTTGTTTTAATTCAATCATAAGATTTTGAAGTGAAGTTTTGGTAATTGTAGGCAGGAGAGCCATTGTATGTTCTATTGTGCTGTTGGCTGGCAGCTGTGGGAGCTGGTTTGCTTAAAACAGAGGAGATTTTGGAGGGAGTGGCAAGGCTCAGGATTTCAGATGATATTGAATTTGAAGAGGAGACTTTCCTTTCCATGATGAATGAGGCTAGAGAGGTTGGCTAATCTTGCTCTTACATCATCTTTTTTTATATTTGTTTCGAATTCTATTTTTCGTCCTAAATTTTTACATTTATTTTTAGTTAAACCACACGTTTAGTCTCTGAATTTTTAAATCTGTATATAATATATAATTAATGTCTGAACTTTAAAAAGTATCTAATGCGTTGTGTTAATGGGTTTGTAGATTTTTTTAAAAAATGTCTGATAGGTCAGGACTATTCGACAAGAAAAAAAAAAGAAGAAAGCTTAGAGACCTATTAGACGAGATTGAAAGTGATGAAAGTTCTTAGACTAAACTTGTAATTCAACCTTTGTCTTATTTTAGTTTTTGAACTTTTAAAATATATATTTTAATCTCAAAAAATGATCCTTTTGATCGTCATCATTGTCATTGCTCTACCGTCAACTTTCTCATTGTCTTTTCCCTTATTGTTGGTCTATAATCAACCCTAATTAGTATTTTAATACATATTTGAATCCACGTTATTCGTTTGTTAAACAATCGATAGCAGTAATAATCGAAATTATTTAATTATTTATTTTTAAAAATTCACGGACTAAAGTGAATATTTCAAAAGTTCACGATCAGAATAGAATGAACATGTAATTCAACGATCAAAATGAGATTTAAACTTTTGCATTCTTCTAATGAAAGCTATAAGCGCCGTAGTCATCTTACACCAACTAACAATATACATTCTTCACAATTGTCGTTCACTTCCAGAGACGAGGAAAAGTAAAAGCTGCTACTCCAAACATCCCGATGCAGGTCCGAGTCGAGAAAGCTCTCGACGCTATCTATGTCTGTTCGTTCGGGAGAGATCCGATAGAGGAAGACGATGAGAAACTGATAGTAATTATGCTAAGTGCTGTGTTTCCATCGGTTGGGCAAACTGAGATTCAAAGGATTGTGAAAGAGAAGGCAGTTAGAATTGCTGAAGGCAAAGACAGTGCCATTGTTCCTGAGCCAAAGCCTTTGTCAAAAGAAGCCATTTTGCTGCAAATGAAGGACCTCCAATTCCTCCAAGAGAAGAGTGAGACT

mRNA sequence

ATGTCCCTGTCCTCTACTTCCACTTCATCTTCTTTCCGCCCAAATCTCAATTCCATTTCCATTCCCAATCCCAGAACCTGCTTCCTCTGTAATCCGATTCCGACTTCTCGGGCGATTCTCCTCCTCCGCCGCCGCCGAGCCTTGTCCGTGAGATCGTACATGGAGAATCCGAATTCTCTGTCCGGATTCGCGAACAAGGTCATCGGTTCTCTCCCGGTGGTCGGCCTGATCGCCAGGATTCTCAGCGACGAAGGCGGCGTCGGCACCGACATTATCGATTTCGCCGAGTTTCGGAGACGAGTTGGGAAGAAATGTACGGTCATGGATTCTAGGGCTTTCTACGAATTTCAAAACAGAAGAGGCAAGGCAGGAGAGCCATTGTATGTTCTATTGTGCTGTTGGCTGGCAGCTGTGGGAGCTGGTTTGCTTAAAACAGAGGAGATTTTGGAGGGAGTGGCAAGGCTCAGGATTTCAGATGATATTGAATTTGAAGAGGAGACTTTCCTTTCCATGATGAATGAGGCTAGAGAGAGACGAGGAAAAGTAAAAGCTGCTACTCCAAACATCCCGATGCAGGTCCGAGTCGAGAAAGCTCTCGACGCTATCTATGTCTGTTCGTTCGGGAGAGATCCGATAGAGGAAGACGATGAGAAACTGATAGTAATTATGCTAAGTGCTGTGTTTCCATCGGTTGGGCAAACTGAGATTCAAAGGATTGTGAAAGAGAAGGCAGTTAGAATTGCTGAAGGCAAAGACAGTGCCATTGTTCCTGAGCCAAAGCCTTTGTCAAAAGAAGCCATTTTGCTGCAAATGAAGGACCTCCAATTCCTCCAAGAGAAGAGTGAGACT

Coding sequence (CDS)

ATGTCCCTGTCCTCTACTTCCACTTCATCTTCTTTCCGCCCAAATCTCAATTCCATTTCCATTCCCAATCCCAGAACCTGCTTCCTCTGTAATCCGATTCCGACTTCTCGGGCGATTCTCCTCCTCCGCCGCCGCCGAGCCTTGTCCGTGAGATCGTACATGGAGAATCCGAATTCTCTGTCCGGATTCGCGAACAAGGTCATCGGTTCTCTCCCGGTGGTCGGCCTGATCGCCAGGATTCTCAGCGACGAAGGCGGCGTCGGCACCGACATTATCGATTTCGCCGAGTTTCGGAGACGAGTTGGGAAGAAATGTACGGTCATGGATTCTAGGGCTTTCTACGAATTTCAAAACAGAAGAGGCAAGGCAGGAGAGCCATTGTATGTTCTATTGTGCTGTTGGCTGGCAGCTGTGGGAGCTGGTTTGCTTAAAACAGAGGAGATTTTGGAGGGAGTGGCAAGGCTCAGGATTTCAGATGATATTGAATTTGAAGAGGAGACTTTCCTTTCCATGATGAATGAGGCTAGAGAGAGACGAGGAAAAGTAAAAGCTGCTACTCCAAACATCCCGATGCAGGTCCGAGTCGAGAAAGCTCTCGACGCTATCTATGTCTGTTCGTTCGGGAGAGATCCGATAGAGGAAGACGATGAGAAACTGATAGTAATTATGCTAAGTGCTGTGTTTCCATCGGTTGGGCAAACTGAGATTCAAAGGATTGTGAAAGAGAAGGCAGTTAGAATTGCTGAAGGCAAAGACAGTGCCATTGTTCCTGAGCCAAAGCCTTTGTCAAAAGAAGCCATTTTGCTGCAAATGAAGGACCTCCAATTCCTCCAAGAGAAGAGTGAGACT

Protein sequence

MSLSSTSTSSSFRPNLNSISIPNPRTCFLCNPIPTSRAILLLRRRRALSVRSYMENPNSLSGFANKVIGSLPVVGLIARILSDEGGVGTDIIDFAEFRRRVGKKCTVMDSRAFYEFQNRRGKAGEPLYVLLCCWLAAVGAGLLKTEEILEGVARLRISDDIEFEEETFLSMMNEARERRGKVKAATPNIPMQVRVEKALDAIYVCSFGRDPIEEDDEKLIVIMLSAVFPSVGQTEIQRIVKEKAVRIAEGKDSAIVPEPKPLSKEAILLQMKDLQFLQEKSET
Homology
BLAST of MS016496 vs. NCBI nr
Match: XP_022158919.1 (photosystem I assembly factor PSA3, chloroplastic [Momordica charantia])

HSP 1 Score: 537.0 bits (1382), Expect = 1.0e-148
Identity = 281/283 (99.29%), Postives = 281/283 (99.29%), Query Frame = 0

Query: 1   MSLSSTSTSSSFRPNLNSISIPNPRTCFLCNPIPTSRAILLLRRRRALSVRSYMENPNSL 60
           MSLSSTSTSSSFRPNLNSISIP PRTCFLCNPIPTS AILLLRRRRALSVRSYMENPNSL
Sbjct: 1   MSLSSTSTSSSFRPNLNSISIPKPRTCFLCNPIPTSPAILLLRRRRALSVRSYMENPNSL 60

Query: 61  SGFANKVIGSLPVVGLIARILSDEGGVGTDIIDFAEFRRRVGKKCTVMDSRAFYEFQNRR 120
           SGFANKVIGSLPVVGLIARILSDEGGVGTDIIDFAEFRRRVGKKCTVMDSRAFYEFQNRR
Sbjct: 61  SGFANKVIGSLPVVGLIARILSDEGGVGTDIIDFAEFRRRVGKKCTVMDSRAFYEFQNRR 120

Query: 121 GKAGEPLYVLLCCWLAAVGAGLLKTEEILEGVARLRISDDIEFEEETFLSMMNEARERRG 180
           GKAGEPLYVLLCCWLAAVGAGLLKTEEILEGVARLRISDDIEFEEETFLSMMNEARERRG
Sbjct: 121 GKAGEPLYVLLCCWLAAVGAGLLKTEEILEGVARLRISDDIEFEEETFLSMMNEARERRG 180

Query: 181 KVKAATPNIPMQVRVEKALDAIYVCSFGRDPIEEDDEKLIVIMLSAVFPSVGQTEIQRIV 240
           KVKAATPNIPMQVRVEKALDAIYVCSFGRDPIEEDDEKLIVIMLSAVFPSVGQTEIQRIV
Sbjct: 181 KVKAATPNIPMQVRVEKALDAIYVCSFGRDPIEEDDEKLIVIMLSAVFPSVGQTEIQRIV 240

Query: 241 KEKAVRIAEGKDSAIVPEPKPLSKEAILLQMKDLQFLQEKSET 284
           KEKAVRIAEGKDSAIVPEPKPLSKEAILLQMKDLQFLQEKSET
Sbjct: 241 KEKAVRIAEGKDSAIVPEPKPLSKEAILLQMKDLQFLQEKSET 283

BLAST of MS016496 vs. NCBI nr
Match: XP_008443515.1 (PREDICTED: uncharacterized protein LOC103487086 [Cucumis melo] >KAA0053674.1 putative ubiquinone biosynthesis UbiB [Cucumis melo var. makuwa] >TYK14001.1 putative ubiquinone biosynthesis UbiB [Cucumis melo var. makuwa])

HSP 1 Score: 432.6 bits (1111), Expect = 2.7e-117
Identity = 231/291 (79.38%), Postives = 261/291 (89.69%), Query Frame = 0

Query: 1   MSLSSTSTSSSFRPNLNSISIPNPRTCFLCNPIPTSRAIL--LLRRRR------ALSVRS 60
           MS++STST+SS   +L+S    NP++ FL NPI  SR  L  L  RRR      A S+++
Sbjct: 1   MSVTSTSTTSS---SLHS----NPKSFFLSNPISPSRLFLPQLYGRRRSSNSAGAFSIKA 60

Query: 61  YMENPNSLSGFANKVIGSLPVVGLIARILSDEGGVGTDIIDFAEFRRRVGKKCTVMDSRA 120
           YMENPNSLSGFA+KVIGSLPVVGLIARI+SDEGGVG+D+IDFAEFRRRVGKKCT+M+S+A
Sbjct: 61  YMENPNSLSGFASKVIGSLPVVGLIARIMSDEGGVGSDLIDFAEFRRRVGKKCTIMESKA 120

Query: 121 FYEFQNRRGKAGEPLYVLLCCWLAAVGAGLLKTEEILEGVARLRISDDIEFEEETFLSMM 180
           FYEFQ R+GKAGEPLYVLLCCWLAAVGAGLLK+EEILEGVARLRIS+DIEFEEETF++MM
Sbjct: 121 FYEFQERKGKAGEPLYVLLCCWLAAVGAGLLKSEEILEGVARLRISNDIEFEEETFIAMM 180

Query: 181 NEARERRGKVKAATPNIPMQVRVEKALDAIYVCSFGRDPIEEDDEKLIVIMLSAVFPSVG 240
           NEARE+R K+KAA PNIPM+VRVEKALDAIYVCSFGRDPIEEDDEKL+VIMLSAVFPSVG
Sbjct: 181 NEAREKREKLKAAVPNIPMEVRVEKALDAIYVCSFGRDPIEEDDEKLLVIMLSAVFPSVG 240

Query: 241 QTEIQRIVKEKAVRIAEGKDSAIVPEPKPLSKEAILLQMKDLQFLQEKSET 284
           + EIQRIVKEKA+RIAEGKD AIVPEP+PLSKEAIL QMKDLQFLQEK+ET
Sbjct: 241 EIEIQRIVKEKAIRIAEGKDIAIVPEPQPLSKEAILAQMKDLQFLQEKNET 284

BLAST of MS016496 vs. NCBI nr
Match: XP_004141087.1 (photosystem I assembly factor PSA3, chloroplastic [Cucumis sativus] >KGN59665.1 hypothetical protein Csa_000816 [Cucumis sativus])

HSP 1 Score: 428.3 bits (1100), Expect = 5.1e-116
Identity = 230/291 (79.04%), Postives = 259/291 (89.00%), Query Frame = 0

Query: 1   MSLSSTSTSSSFRPNLNSISIPNPRTCFLCNPIPTSRAIL--LLRRRR------ALSVRS 60
           MS++STST+SS   +L+S    NP+  FL NPI  S   L  L  RRR      A S+++
Sbjct: 1   MSVTSTSTTSS---SLHS----NPKFFFLSNPISPSPLFLRKLYGRRRNPNSAGAFSIKA 60

Query: 61  YMENPNSLSGFANKVIGSLPVVGLIARILSDEGGVGTDIIDFAEFRRRVGKKCTVMDSRA 120
           YME+PNSLSGFA+KVIGSLPVVGLIARILSDEGGVGTDIIDFAEFRRRVGKKCT+M+S+A
Sbjct: 61  YMESPNSLSGFASKVIGSLPVVGLIARILSDEGGVGTDIIDFAEFRRRVGKKCTIMESKA 120

Query: 121 FYEFQNRRGKAGEPLYVLLCCWLAAVGAGLLKTEEILEGVARLRISDDIEFEEETFLSMM 180
           FYEFQ R+GKAGEPLYVLLCCWLAAVGAGLLK+EEILEGVARLRIS+DIEFEEETFL+MM
Sbjct: 121 FYEFQERKGKAGEPLYVLLCCWLAAVGAGLLKSEEILEGVARLRISNDIEFEEETFLAMM 180

Query: 181 NEARERRGKVKAATPNIPMQVRVEKALDAIYVCSFGRDPIEEDDEKLIVIMLSAVFPSVG 240
           NEARE+R K+KAA PNIPM+VR+EKALDAIYVCSFGRDPIEEDDEKL+VIMLSAVFPSVG
Sbjct: 181 NEAREKREKLKAAVPNIPMEVRIEKALDAIYVCSFGRDPIEEDDEKLLVIMLSAVFPSVG 240

Query: 241 QTEIQRIVKEKAVRIAEGKDSAIVPEPKPLSKEAILLQMKDLQFLQEKSET 284
           + EIQRI+KEKA+RIAEGKD+AIVPEP+PLSKEAIL QMKDLQFLQE +ET
Sbjct: 241 EIEIQRILKEKAIRIAEGKDTAIVPEPQPLSKEAILAQMKDLQFLQENNET 284

BLAST of MS016496 vs. NCBI nr
Match: XP_038905081.1 (photosystem I assembly factor PSA3, chloroplastic [Benincasa hispida])

HSP 1 Score: 425.6 bits (1093), Expect = 3.3e-115
Identity = 232/292 (79.45%), Postives = 253/292 (86.64%), Query Frame = 0

Query: 1   MSLSST-STSSSFRPNLNSISIPNPRTCFLCNPIPTSRAIL--LLRRRR------ALSVR 60
           MS++ST STSSS   NL S         FL  PI  SR  L  +  RRR       LSV+
Sbjct: 1   MSVTSTSSTSSSLHSNLKSF--------FLSKPISPSRLFLRQIYGRRRQSNSAGVLSVK 60

Query: 61  SYMENPNSLSGFANKVIGSLPVVGLIARILSDEGGVGTDIIDFAEFRRRVGKKCTVMDSR 120
           +YMENPNSLSGFANKVIGSLPVVGLIARILSDEGGVG+DIIDFAEFRRRVGKKCT+M+S+
Sbjct: 61  AYMENPNSLSGFANKVIGSLPVVGLIARILSDEGGVGSDIIDFAEFRRRVGKKCTIMESK 120

Query: 121 AFYEFQNRRGKAGEPLYVLLCCWLAAVGAGLLKTEEILEGVARLRISDDIEFEEETFLSM 180
           AFYEFQ R+GKAGEPLYVLLCCWLAAVGAGLLK+EEILEGVARLRIS+DIEFEEETFL+M
Sbjct: 121 AFYEFQERKGKAGEPLYVLLCCWLAAVGAGLLKSEEILEGVARLRISNDIEFEEETFLAM 180

Query: 181 MNEARERRGKVKAATPNIPMQVRVEKALDAIYVCSFGRDPIEEDDEKLIVIMLSAVFPSV 240
           MNEARE+R K+K A PNIPM+VRVEKALDAIYVCSFGRDPIEEDDEKL+VIMLSAVF SV
Sbjct: 181 MNEAREKREKLKVAVPNIPMEVRVEKALDAIYVCSFGRDPIEEDDEKLLVIMLSAVFASV 240

Query: 241 GQTEIQRIVKEKAVRIAEGKDSAIVPEPKPLSKEAILLQMKDLQFLQEKSET 284
            +TEIQRIVKEKA+RIAEGKD AIVPEP+PLSKEAIL QMKDLQFLQEK+ET
Sbjct: 241 RETEIQRIVKEKAIRIAEGKDIAIVPEPQPLSKEAILAQMKDLQFLQEKTET 284

BLAST of MS016496 vs. NCBI nr
Match: KAG6588962.1 (Photosystem I assembly factor PSA3, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 421.8 bits (1083), Expect = 4.7e-114
Identity = 227/294 (77.21%), Postives = 253/294 (86.05%), Query Frame = 0

Query: 1   MSLSSTSTSSSFRPNLNSISIPNPRTCFLCNPIPTSRAI------LLLRRRR-----ALS 60
           M+++STST+SSF          NP++ FL + I   + +       + RRR       LS
Sbjct: 1   MAVTSTSTTSSFH--------SNPKSFFLSSLISPPKFLHRQFHGSVGRRRHPTSTGVLS 60

Query: 61  VRSYMENPNSLSGFANKVIGSLPVVGLIARILSDEGGVGTDIIDFAEFRRRVGKKCTVMD 120
           VR+YMENPNSLSGFANKVIGSLPVVGLIARILSDEGGVG+DIIDFAEFRRRVGKKCT+MD
Sbjct: 61  VRAYMENPNSLSGFANKVIGSLPVVGLIARILSDEGGVGSDIIDFAEFRRRVGKKCTIMD 120

Query: 121 SRAFYEFQNRRGKAGEPLYVLLCCWLAAVGAGLLKTEEILEGVARLRISDDIEFEEETFL 180
           S+AFY+FQ RRGK GEPL+VLLCCWLAAVGAGLLK+EEILEGVARLRISDDIEFEEETFL
Sbjct: 121 SKAFYDFQERRGKPGEPLFVLLCCWLAAVGAGLLKSEEILEGVARLRISDDIEFEEETFL 180

Query: 181 SMMNEARERRGKVKAATPNIPMQVRVEKALDAIYVCSFGRDPIEEDDEKLIVIMLSAVFP 240
           +MMNEARE+R K+K A P IPM+VRVEKALDAIYVCSFGRDPIEEDDEKL+VIMLS+VFP
Sbjct: 181 AMMNEAREKREKLKVAVPKIPMEVRVEKALDAIYVCSFGRDPIEEDDEKLLVIMLSSVFP 240

Query: 241 SVGQTEIQRIVKEKAVRIAEGKDSAIVPEPKPLSKEAILLQMKDLQFLQEKSET 284
           +VGQ+EIQRIVKEKAVRIAEGKD AIVPEP+PLSKEAIL QMKDLQFLQE SET
Sbjct: 241 TVGQSEIQRIVKEKAVRIAEGKDIAIVPEPQPLSKEAILAQMKDLQFLQENSET 286

BLAST of MS016496 vs. ExPASy Swiss-Prot
Match: Q9M3C6 (Photosystem I assembly factor PSA3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PSA3 PE=1 SV=1)

HSP 1 Score: 321.6 bits (823), Expect = 8.7e-87
Identity = 165/253 (65.22%), Postives = 203/253 (80.24%), Query Frame = 0

Query: 29  LCNPIPTSRAILLLRRRRALSVRSYMENPNSLSGFANKVIGSLPVVGLIARILSDEGGVG 88
           L NP  TS +   L    ALS+R+Y+E PNS S FANKVIGSLPV+GL+ARI+SDEGGVG
Sbjct: 23  LRNPSTTSSSRPKLDGGFALSIRAYIEKPNSFSTFANKVIGSLPVIGLLARIISDEGGVG 82

Query: 89  TDIIDFAEFRRRVGKKCTVMDSRAFYEFQNRRGKAGEPLYVLLCCWLAAVGAGLLKTEEI 148
            D++DFAEFR+RVG KCT  DSRAFYEFQ RRGKAGEPLYVLLCCW+AAVGAGLLK+EEI
Sbjct: 83  RDLVDFAEFRKRVGNKCTPDDSRAFYEFQQRRGKAGEPLYVLLCCWVAAVGAGLLKSEEI 142

Query: 149 LEGVARLRISDDIEFEEETFLSMMNEARERRGKVKAATPNIPMQVRVEKALDAIYVCSFG 208
           LEGV R+ IS+D+EFEE+ F+++M EAR+RR K+    P IPM++RVEKAL+ IY C F 
Sbjct: 143 LEGVTRVSISNDLEFEEQNFIALMTEARQRRAKLNIDAPTIPMELRVEKALEGIYACCFR 202

Query: 209 RDPIEEDDEKLIVIMLSAVFPSVGQTEIQRIVKEKAVRIAEGKDSAIVPEPKPLSKEAIL 268
           R  IEE+DEKL+ +ML AVFPSV ++EI+RI+KEKA R+ EG +   V   K L KEAI 
Sbjct: 203 RGLIEEEDEKLLKVMLIAVFPSVEKSEIERIIKEKATRVEEGGEEENV-MAKRLPKEAIQ 262

Query: 269 LQMKDLQFLQEKS 282
           +QMKDL+FLQ+++
Sbjct: 263 MQMKDLEFLQQQN 274

BLAST of MS016496 vs. ExPASy Swiss-Prot
Match: B4FSG1 (Photosystem I assembly factor PSA3, chloroplastic OS=Zea mays OX=4577 GN=PSA3 PE=2 SV=1)

HSP 1 Score: 301.6 bits (771), Expect = 9.4e-81
Identity = 150/238 (63.03%), Postives = 192/238 (80.67%), Query Frame = 0

Query: 43  RRRRALSVRSYME-NPNSLSGFANKVIGSLPVVGLIARILSDEGGVGTDIIDFAEFRRRV 102
           RRRR  +V +YME NPNS +  A +++G+LP+VGL+ARIL+DEGGVG DIIDFAEFRRRV
Sbjct: 29  RRRRYRAVVAYMEPNPNSPAAIAGRLVGALPIVGLVARILNDEGGVGGDIIDFAEFRRRV 88

Query: 103 GKKCTVMDSRAFYEFQNRRGKAGEPLYVLLCCWLAAVGAGLLKTEEILEGVARLRISDDI 162
            KKCTVMDS+AFY+F  RRGK G+P YVLLCCWLAA+GAGLLKTEEILEGVARLRIS+DI
Sbjct: 89  SKKCTVMDSKAFYDFNQRRGKPGDPFYVLLCCWLAAIGAGLLKTEEILEGVARLRISNDI 148

Query: 163 EFEEETFLSMMNEARERRGKVKAATPNIPMQVRVEKALDAIYVCSFGRDPIEEDDEKLIV 222
           EFEEETF+ MM  A+E+R K+KA  P IPM+ R EKAL+AIYVC FG+D +E++DEKL+ 
Sbjct: 149 EFEEETFIDMMRAAKEKRAKLKAPAPQIPMETRAEKALEAIYVCCFGQDMVEDEDEKLLR 208

Query: 223 IMLSAVFPSVGQTEIQRIVKEKAVRIAEGKDSAIVPEPKPLSKEAILLQMKDLQFLQE 280
            +L+AVFPSVG+  ++R+V   A ++A G+      + + +SKE    Q+KDL+FL++
Sbjct: 209 AILNAVFPSVGRAAVERMVASMAKQVASGERKR---DGRTVSKEVQQRQLKDLEFLKQ 263

BLAST of MS016496 vs. ExPASy TrEMBL
Match: A0A6J1E0U9 (photosystem I assembly factor PSA3, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111025378 PE=4 SV=1)

HSP 1 Score: 537.0 bits (1382), Expect = 4.9e-149
Identity = 281/283 (99.29%), Postives = 281/283 (99.29%), Query Frame = 0

Query: 1   MSLSSTSTSSSFRPNLNSISIPNPRTCFLCNPIPTSRAILLLRRRRALSVRSYMENPNSL 60
           MSLSSTSTSSSFRPNLNSISIP PRTCFLCNPIPTS AILLLRRRRALSVRSYMENPNSL
Sbjct: 1   MSLSSTSTSSSFRPNLNSISIPKPRTCFLCNPIPTSPAILLLRRRRALSVRSYMENPNSL 60

Query: 61  SGFANKVIGSLPVVGLIARILSDEGGVGTDIIDFAEFRRRVGKKCTVMDSRAFYEFQNRR 120
           SGFANKVIGSLPVVGLIARILSDEGGVGTDIIDFAEFRRRVGKKCTVMDSRAFYEFQNRR
Sbjct: 61  SGFANKVIGSLPVVGLIARILSDEGGVGTDIIDFAEFRRRVGKKCTVMDSRAFYEFQNRR 120

Query: 121 GKAGEPLYVLLCCWLAAVGAGLLKTEEILEGVARLRISDDIEFEEETFLSMMNEARERRG 180
           GKAGEPLYVLLCCWLAAVGAGLLKTEEILEGVARLRISDDIEFEEETFLSMMNEARERRG
Sbjct: 121 GKAGEPLYVLLCCWLAAVGAGLLKTEEILEGVARLRISDDIEFEEETFLSMMNEARERRG 180

Query: 181 KVKAATPNIPMQVRVEKALDAIYVCSFGRDPIEEDDEKLIVIMLSAVFPSVGQTEIQRIV 240
           KVKAATPNIPMQVRVEKALDAIYVCSFGRDPIEEDDEKLIVIMLSAVFPSVGQTEIQRIV
Sbjct: 181 KVKAATPNIPMQVRVEKALDAIYVCSFGRDPIEEDDEKLIVIMLSAVFPSVGQTEIQRIV 240

Query: 241 KEKAVRIAEGKDSAIVPEPKPLSKEAILLQMKDLQFLQEKSET 284
           KEKAVRIAEGKDSAIVPEPKPLSKEAILLQMKDLQFLQEKSET
Sbjct: 241 KEKAVRIAEGKDSAIVPEPKPLSKEAILLQMKDLQFLQEKSET 283

BLAST of MS016496 vs. ExPASy TrEMBL
Match: A0A5D3CQC9 (Putative ubiquinone biosynthesis UbiB OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1119G00270 PE=4 SV=1)

HSP 1 Score: 432.6 bits (1111), Expect = 1.3e-117
Identity = 231/291 (79.38%), Postives = 261/291 (89.69%), Query Frame = 0

Query: 1   MSLSSTSTSSSFRPNLNSISIPNPRTCFLCNPIPTSRAIL--LLRRRR------ALSVRS 60
           MS++STST+SS   +L+S    NP++ FL NPI  SR  L  L  RRR      A S+++
Sbjct: 1   MSVTSTSTTSS---SLHS----NPKSFFLSNPISPSRLFLPQLYGRRRSSNSAGAFSIKA 60

Query: 61  YMENPNSLSGFANKVIGSLPVVGLIARILSDEGGVGTDIIDFAEFRRRVGKKCTVMDSRA 120
           YMENPNSLSGFA+KVIGSLPVVGLIARI+SDEGGVG+D+IDFAEFRRRVGKKCT+M+S+A
Sbjct: 61  YMENPNSLSGFASKVIGSLPVVGLIARIMSDEGGVGSDLIDFAEFRRRVGKKCTIMESKA 120

Query: 121 FYEFQNRRGKAGEPLYVLLCCWLAAVGAGLLKTEEILEGVARLRISDDIEFEEETFLSMM 180
           FYEFQ R+GKAGEPLYVLLCCWLAAVGAGLLK+EEILEGVARLRIS+DIEFEEETF++MM
Sbjct: 121 FYEFQERKGKAGEPLYVLLCCWLAAVGAGLLKSEEILEGVARLRISNDIEFEEETFIAMM 180

Query: 181 NEARERRGKVKAATPNIPMQVRVEKALDAIYVCSFGRDPIEEDDEKLIVIMLSAVFPSVG 240
           NEARE+R K+KAA PNIPM+VRVEKALDAIYVCSFGRDPIEEDDEKL+VIMLSAVFPSVG
Sbjct: 181 NEAREKREKLKAAVPNIPMEVRVEKALDAIYVCSFGRDPIEEDDEKLLVIMLSAVFPSVG 240

Query: 241 QTEIQRIVKEKAVRIAEGKDSAIVPEPKPLSKEAILLQMKDLQFLQEKSET 284
           + EIQRIVKEKA+RIAEGKD AIVPEP+PLSKEAIL QMKDLQFLQEK+ET
Sbjct: 241 EIEIQRIVKEKAIRIAEGKDIAIVPEPQPLSKEAILAQMKDLQFLQEKNET 284

BLAST of MS016496 vs. ExPASy TrEMBL
Match: A0A1S3B876 (uncharacterized protein LOC103487086 OS=Cucumis melo OX=3656 GN=LOC103487086 PE=4 SV=1)

HSP 1 Score: 432.6 bits (1111), Expect = 1.3e-117
Identity = 231/291 (79.38%), Postives = 261/291 (89.69%), Query Frame = 0

Query: 1   MSLSSTSTSSSFRPNLNSISIPNPRTCFLCNPIPTSRAIL--LLRRRR------ALSVRS 60
           MS++STST+SS   +L+S    NP++ FL NPI  SR  L  L  RRR      A S+++
Sbjct: 1   MSVTSTSTTSS---SLHS----NPKSFFLSNPISPSRLFLPQLYGRRRSSNSAGAFSIKA 60

Query: 61  YMENPNSLSGFANKVIGSLPVVGLIARILSDEGGVGTDIIDFAEFRRRVGKKCTVMDSRA 120
           YMENPNSLSGFA+KVIGSLPVVGLIARI+SDEGGVG+D+IDFAEFRRRVGKKCT+M+S+A
Sbjct: 61  YMENPNSLSGFASKVIGSLPVVGLIARIMSDEGGVGSDLIDFAEFRRRVGKKCTIMESKA 120

Query: 121 FYEFQNRRGKAGEPLYVLLCCWLAAVGAGLLKTEEILEGVARLRISDDIEFEEETFLSMM 180
           FYEFQ R+GKAGEPLYVLLCCWLAAVGAGLLK+EEILEGVARLRIS+DIEFEEETF++MM
Sbjct: 121 FYEFQERKGKAGEPLYVLLCCWLAAVGAGLLKSEEILEGVARLRISNDIEFEEETFIAMM 180

Query: 181 NEARERRGKVKAATPNIPMQVRVEKALDAIYVCSFGRDPIEEDDEKLIVIMLSAVFPSVG 240
           NEARE+R K+KAA PNIPM+VRVEKALDAIYVCSFGRDPIEEDDEKL+VIMLSAVFPSVG
Sbjct: 181 NEAREKREKLKAAVPNIPMEVRVEKALDAIYVCSFGRDPIEEDDEKLLVIMLSAVFPSVG 240

Query: 241 QTEIQRIVKEKAVRIAEGKDSAIVPEPKPLSKEAILLQMKDLQFLQEKSET 284
           + EIQRIVKEKA+RIAEGKD AIVPEP+PLSKEAIL QMKDLQFLQEK+ET
Sbjct: 241 EIEIQRIVKEKAIRIAEGKDIAIVPEPQPLSKEAILAQMKDLQFLQEKNET 284

BLAST of MS016496 vs. ExPASy TrEMBL
Match: A0A0A0LCC2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G836490 PE=4 SV=1)

HSP 1 Score: 428.3 bits (1100), Expect = 2.4e-116
Identity = 230/291 (79.04%), Postives = 259/291 (89.00%), Query Frame = 0

Query: 1   MSLSSTSTSSSFRPNLNSISIPNPRTCFLCNPIPTSRAIL--LLRRRR------ALSVRS 60
           MS++STST+SS   +L+S    NP+  FL NPI  S   L  L  RRR      A S+++
Sbjct: 1   MSVTSTSTTSS---SLHS----NPKFFFLSNPISPSPLFLRKLYGRRRNPNSAGAFSIKA 60

Query: 61  YMENPNSLSGFANKVIGSLPVVGLIARILSDEGGVGTDIIDFAEFRRRVGKKCTVMDSRA 120
           YME+PNSLSGFA+KVIGSLPVVGLIARILSDEGGVGTDIIDFAEFRRRVGKKCT+M+S+A
Sbjct: 61  YMESPNSLSGFASKVIGSLPVVGLIARILSDEGGVGTDIIDFAEFRRRVGKKCTIMESKA 120

Query: 121 FYEFQNRRGKAGEPLYVLLCCWLAAVGAGLLKTEEILEGVARLRISDDIEFEEETFLSMM 180
           FYEFQ R+GKAGEPLYVLLCCWLAAVGAGLLK+EEILEGVARLRIS+DIEFEEETFL+MM
Sbjct: 121 FYEFQERKGKAGEPLYVLLCCWLAAVGAGLLKSEEILEGVARLRISNDIEFEEETFLAMM 180

Query: 181 NEARERRGKVKAATPNIPMQVRVEKALDAIYVCSFGRDPIEEDDEKLIVIMLSAVFPSVG 240
           NEARE+R K+KAA PNIPM+VR+EKALDAIYVCSFGRDPIEEDDEKL+VIMLSAVFPSVG
Sbjct: 181 NEAREKREKLKAAVPNIPMEVRIEKALDAIYVCSFGRDPIEEDDEKLLVIMLSAVFPSVG 240

Query: 241 QTEIQRIVKEKAVRIAEGKDSAIVPEPKPLSKEAILLQMKDLQFLQEKSET 284
           + EIQRI+KEKA+RIAEGKD+AIVPEP+PLSKEAIL QMKDLQFLQE +ET
Sbjct: 241 EIEIQRILKEKAIRIAEGKDTAIVPEPQPLSKEAILAQMKDLQFLQENNET 284

BLAST of MS016496 vs. ExPASy TrEMBL
Match: A0A6J1EQR9 (photosystem I assembly factor PSA3, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111435039 PE=4 SV=1)

HSP 1 Score: 420.2 bits (1079), Expect = 6.7e-114
Identity = 226/294 (76.87%), Postives = 253/294 (86.05%), Query Frame = 0

Query: 1   MSLSSTSTSSSFRPNLNSISIPNPRTCFLCNPIPTSRAI------LLLRRRR-----ALS 60
           M+++STST+SSF          NP++ FL + I   + +       + RRR       LS
Sbjct: 1   MAVTSTSTTSSFH--------SNPKSFFLYSLISPPKLLHRQFHGSVGRRRHPTSTGVLS 60

Query: 61  VRSYMENPNSLSGFANKVIGSLPVVGLIARILSDEGGVGTDIIDFAEFRRRVGKKCTVMD 120
           VR+YMENPNSLSGFANKVIGSLPVVGLIARILSDEGGVG+DIIDFAEFRRRVGKKCT+MD
Sbjct: 61  VRAYMENPNSLSGFANKVIGSLPVVGLIARILSDEGGVGSDIIDFAEFRRRVGKKCTIMD 120

Query: 121 SRAFYEFQNRRGKAGEPLYVLLCCWLAAVGAGLLKTEEILEGVARLRISDDIEFEEETFL 180
           S+AFY+FQ RRGK GEPL+VLLCCWLAAVGAGLLK+EEILEGVARLRISDDIEFEEETFL
Sbjct: 121 SKAFYDFQERRGKPGEPLFVLLCCWLAAVGAGLLKSEEILEGVARLRISDDIEFEEETFL 180

Query: 181 SMMNEARERRGKVKAATPNIPMQVRVEKALDAIYVCSFGRDPIEEDDEKLIVIMLSAVFP 240
           +MMNEARE+R K+K A P IPM+VRVEKALDAIYVCSFGRDPIEEDDEKL+VIMLS+VFP
Sbjct: 181 AMMNEAREKREKLKVAVPKIPMEVRVEKALDAIYVCSFGRDPIEEDDEKLLVIMLSSVFP 240

Query: 241 SVGQTEIQRIVKEKAVRIAEGKDSAIVPEPKPLSKEAILLQMKDLQFLQEKSET 284
           +VGQ+EIQRIVKEKAVRIAEGKD AIVPEP+PLSKEAIL QMKDLQFL+E SET
Sbjct: 241 TVGQSEIQRIVKEKAVRIAEGKDIAIVPEPQPLSKEAILAQMKDLQFLRENSET 286

BLAST of MS016496 vs. TAIR 10
Match: AT3G55250.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: mitochondrion, chloroplast, nucleus; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; Has 46 Blast hits to 46 proteins in 17 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 46; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 321.6 bits (823), Expect = 6.2e-88
Identity = 165/253 (65.22%), Postives = 203/253 (80.24%), Query Frame = 0

Query: 29  LCNPIPTSRAILLLRRRRALSVRSYMENPNSLSGFANKVIGSLPVVGLIARILSDEGGVG 88
           L NP  TS +   L    ALS+R+Y+E PNS S FANKVIGSLPV+GL+ARI+SDEGGVG
Sbjct: 23  LRNPSTTSSSRPKLDGGFALSIRAYIEKPNSFSTFANKVIGSLPVIGLLARIISDEGGVG 82

Query: 89  TDIIDFAEFRRRVGKKCTVMDSRAFYEFQNRRGKAGEPLYVLLCCWLAAVGAGLLKTEEI 148
            D++DFAEFR+RVG KCT  DSRAFYEFQ RRGKAGEPLYVLLCCW+AAVGAGLLK+EEI
Sbjct: 83  RDLVDFAEFRKRVGNKCTPDDSRAFYEFQQRRGKAGEPLYVLLCCWVAAVGAGLLKSEEI 142

Query: 149 LEGVARLRISDDIEFEEETFLSMMNEARERRGKVKAATPNIPMQVRVEKALDAIYVCSFG 208
           LEGV R+ IS+D+EFEE+ F+++M EAR+RR K+    P IPM++RVEKAL+ IY C F 
Sbjct: 143 LEGVTRVSISNDLEFEEQNFIALMTEARQRRAKLNIDAPTIPMELRVEKALEGIYACCFR 202

Query: 209 RDPIEEDDEKLIVIMLSAVFPSVGQTEIQRIVKEKAVRIAEGKDSAIVPEPKPLSKEAIL 268
           R  IEE+DEKL+ +ML AVFPSV ++EI+RI+KEKA R+ EG +   V   K L KEAI 
Sbjct: 203 RGLIEEEDEKLLKVMLIAVFPSVEKSEIERIIKEKATRVEEGGEEENV-MAKRLPKEAIQ 262

Query: 269 LQMKDLQFLQEKS 282
           +QMKDL+FLQ+++
Sbjct: 263 MQMKDLEFLQQQN 274

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022158919.11.0e-14899.29photosystem I assembly factor PSA3, chloroplastic [Momordica charantia][more]
XP_008443515.12.7e-11779.38PREDICTED: uncharacterized protein LOC103487086 [Cucumis melo] >KAA0053674.1 put... [more]
XP_004141087.15.1e-11679.04photosystem I assembly factor PSA3, chloroplastic [Cucumis sativus] >KGN59665.1 ... [more]
XP_038905081.13.3e-11579.45photosystem I assembly factor PSA3, chloroplastic [Benincasa hispida][more]
KAG6588962.14.7e-11477.21Photosystem I assembly factor PSA3, chloroplastic, partial [Cucurbita argyrosper... [more]
Match NameE-valueIdentityDescription
Q9M3C68.7e-8765.22Photosystem I assembly factor PSA3, chloroplastic OS=Arabidopsis thaliana OX=370... [more]
B4FSG19.4e-8163.03Photosystem I assembly factor PSA3, chloroplastic OS=Zea mays OX=4577 GN=PSA3 PE... [more]
Match NameE-valueIdentityDescription
A0A6J1E0U94.9e-14999.29photosystem I assembly factor PSA3, chloroplastic OS=Momordica charantia OX=3673... [more]
A0A5D3CQC91.3e-11779.38Putative ubiquinone biosynthesis UbiB OS=Cucumis melo var. makuwa OX=1194695 GN=... [more]
A0A1S3B8761.3e-11779.38uncharacterized protein LOC103487086 OS=Cucumis melo OX=3656 GN=LOC103487086 PE=... [more]
A0A0A0LCC22.4e-11679.04Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G836490 PE=4 SV=1[more]
A0A6J1EQR96.7e-11476.87photosystem I assembly factor PSA3, chloroplastic OS=Cucurbita moschata OX=3662 ... [more]
Match NameE-valueIdentityDescription
AT3G55250.16.2e-8865.22unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic... [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR037736Photosystem I assembly factor PSA3PANTHERPTHR36770PHOTOSYSTEM I ASSEMBLY FACTOR PSA3, CHLOROPLASTICcoord: 43..283

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS016496.1MS016496.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0048564 photosystem I assembly