MS016282 (gene) Bitter gourd (TR) v1

Overview


Name	MS016282
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Pectin lyase-like superfamily protein
Location	scaffold1407: 83824 .. 86646 (+)
RNA-Seq Expression	MS016282
Synteny	MS016282

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCTGCGCTTTTTCCTCTTCCTTTTTTTCTTCTCCGCCGCCGCCGCCGCCGCCGCCGACCTCACTACCTGCTCCGGCATAGTCCCCATGAAGTACCGCAACGACAAGATTTCCATCGAGGACTTTGGGGGAGTTGGCGATGGCCGCACATTGAACACTAGGGCTTTTCGGGAGGCCATTTTTCGGATTCAGCATTTGAAGAGAAGAGGGGGCACTCTGCTTTACATTCCTCCCGGGGTTTTCTTGACTGAGAGCTTCAATCTCACCAGCCATATGACTCTCTATCTCGCTAAAGGCGCTGTTATCAAAGCTGTTCAGGTGATTATTCTTGTTGAACTTTGTTCAGTAACATCTTTTTTGTTCAATTGTTCCCATATTCAGCTTGTTTTCTGGGTGATTATTTGTGATTTCCAAATTGAAATAGATGTGGGTCCTGGAGGGGAATGGCGAGAAGTGGTGGGTGGGTCTTTGATTACTCTGTTGCATGTGCATCCACTCTAAATTTATTGCATTATATATGATTGGACCGTTGACAGGCTGTTCTTTGTCGGTCTCTGATGCTAACTTTTGATTACAAAACAATATGATCCCGTTTTATTTGATTGGTCAATTCAGTGTTCTGAAATGCTAATTTGTGGTTATCATTTGGCTGATCTGGACATCAATTTTTAGCAACTCTGGTTGTCAACTTAGTTGAAATTTCTAGTGTGTAAAGTAAACTATGTCGATAACAATGACTATGTAATGAATAGTTTAATTACTTAGACAGTTAGACCATGTCTATTTCAGCCAGCAAAAACTAAAAACGAAGGTCTATTTTATGCCATCTGATCCTTTTCTTAATTCCTTTTGTCTTTCGTAGAGGAATTTTACCAAGAACTTCCCAAGACATGAAGTAAAAACTTGGACATTGTGTAAAGATTTTGTAGTTAGAACCCTGTATATGTTGTTAGGTTCTAAGATATGACTCCTCTATGGTGACTGGCCTACAATAAATTTTCTCTTCTGGTGAGTTTGTGTTTATGTCCATGGATGATCAATTTAGTGGGTGCAAGGTGCAATATCCTCGGTGTTAAGTTTTTCATCATTCACGCTATTGTCTCCGCTATCTTTTCTATGATGTTATTTTCTGCAGATGATGAGACTTTTTCAATCTTCATTTCAGTTAAATAGCTGAAGAATCTTTATCTTGCATTATTAAAAATTTGTATCTTAAGCTTTTTTGTTTATTTGCTTTTGATGGTCCAGGATTCTTCGGATTGGCCCGTAATAGCTCCATTGCCATCTTATGGAAGAGGGAGGGAGCGCCCTGGAGGAAGATATGTGAGCTTTATTCATGGAGATGGCGTTCATGATGTGGTGATCACTGGTATGAATCATCAAGTTCTTCTAAATATAGACGTAATTGCAAACATGGTTTACTCGTGTTCTTGGGCCTGAGAAAAATTGATGTGTCTTAGGCGAGAACGGGACTATTGATGGCCAAGGTGATGCTTGGTGGAATATGTGGAAGACAGGGACTCTCAAGTTCACCAGGCCTAATCTCGTTGAGTTTATCAATTCTTATAATATCATCATTTCCAATGTAATTTTCCAGAATTCTCCATTTTGGAACATCCATCCAGTTTACTGCAGGTAAGAATAAGACTAGCTTAAAGTTAGCTTCAATCATGTTACAGCTCCATATAATAGCATGTATCATGCCTAGAAGACCACTGAAATGTTATCATTGACTAGTTTATCTGTTTAGTCATGTAAAAATTTGAACATCCAACATTTTTAAAGCCTTCTTTCTTACCCAATAATATTTTGATCTCTTCTTTTGGGGTAGTAGAGATATTGTGCAGGGATAAGTGTCCAACATTTCCTCACAATAATCTGCTATGAATCTGCACTGGTATAGTTCTATCTACATTTTTTTTATCTTTTGTTGCAGAAACGTTGTTGTCAGATATGTCACAATATTGGCTCCTCGTGATTCCCCCAACACCGATGGAATTGATCCAGGTGCATTTTTAGATCTCTCGGTACATGTCACATAGTTATTAGCTCCCCCGCCTCCATCTGTGCCCTGGTTCTGGTCACAAGAGCTAGAAAACTCATTAAAATGAAGGGTTATTCTGTATCATAATATTAGTTTTGTATATGATTGGAAGTAACAAAACTTGCAAACTGATGCTCACAGATTCAAGCAATAACGTTTGCATAGAAGATTCCTACATTTCTACTGGAGATGATCTTGTGGCTGTGAAGAGTGGATGGGATGAATATGGTATCGCTTACGGTCGCAGTAGTTACGACATTACTATCCGAAGAGTGTCAGGGTCAAGCCCATTTGCCGGAGTTGCAGTCGGAAGCGAAACCTCCGGTGGAGTGGCAAACGTATTGGCCGAACACCTGAGTTTTTTTGACATGGGAGTTGGTATTAACATCAAGACAAACATTGGAAGGGGAGGGTTCATAAAGAACATAACTGTGTCCAATGTCTACATGGAGCGTTCGAGAAAGGGTCTAAAGATTGCAGGCGATACCGGGGATCATCCAGACGACAAGTTTGACCCAAATGCTCTTCCGATTGTCAAAGACATCACGATTAAAAATGTTTGGGGCGTGAACGTACAGCAAGCAGGTTCTATATATGGCTTGAAGAACTCTCCATTTACCGGTATATGTCTTTCGAACATCAACCTGCACGGCACAACGAGACCGAGATCGGTTCCGTGGACTTGCTCGTATGTAAGTGGTGCAGCTCTTTTGGTTAGTCCATGGCCATGCTCCGAGCTCACCAGCACCCAGCAGGATGGTCTGTGCTCTGATAACTTC

mRNA sequence

ATGCTGCGCTTTTTCCTCTTCCTTTTTTTCTTCTCCGCCGCCGCCGCCGCCGCCGCCGACCTCACTACCTGCTCCGGCATAGTCCCCATGAAGTACCGCAACGACAAGATTTCCATCGAGGACTTTGGGGGAGTTGGCGATGGCCGCACATTGAACACTAGGGCTTTTCGGGAGGCCATTTTTCGGATTCAGCATTTGAAGAGAAGAGGGGGCACTCTGCTTTACATTCCTCCCGGGGTTTTCTTGACTGAGAGCTTCAATCTCACCAGCCATATGACTCTCTATCTCGCTAAAGGCGCTGTTATCAAAGCTGTTCAGGATTCTTCGGATTGGCCCGTAATAGCTCCATTGCCATCTTATGGAAGAGGGAGGGAGCGCCCTGGAGGAAGATATGTGAGCTTTATTCATGGAGATGGCGTTCATGATGTGGTGATCACTGGCGAGAACGGGACTATTGATGGCCAAGGTGATGCTTGGTGGAATATGTGGAAGACAGGGACTCTCAAGTTCACCAGGCCTAATCTCGTTGAGTTTATCAATTCTTATAATATCATCATTTCCAATGTAATTTTCCAGAATTCTCCATTTTGGAACATCCATCCAGTTTACTGCAGAAACGTTGTTGTCAGATATGTCACAATATTGGCTCCTCGTGATTCCCCCAACACCGATGGAATTGATCCAGATTCAAGCAATAACGTTTGCATAGAAGATTCCTACATTTCTACTGGAGATGATCTTGTGGCTGTGAAGAGTGGATGGGATGAATATGGTATCGCTTACGGTCGCAGTAGTTACGACATTACTATCCGAAGAGTGTCAGGGTCAAGCCCATTTGCCGGAGTTGCAGTCGGAAGCGAAACCTCCGGTGGAGTGGCAAACGTATTGGCCGAACACCTGAGTTTTTTTGACATGGGAGTTGGTATTAACATCAAGACAAACATTGGAAGGGGAGGGTTCATAAAGAACATAACTGTGTCCAATGTCTACATGGAGCGTTCGAGAAAGGGTCTAAAGATTGCAGGCGATACCGGGGATCATCCAGACGACAAGTTTGACCCAAATGCTCTTCCGATTGTCAAAGACATCACGATTAAAAATGTTTGGGGCGTGAACGTACAGCAAGCAGGTTCTATATATGGCTTGAAGAACTCTCCATTTACCGGTATATGTCTTTCGAACATCAACCTGCACGGCACAACGAGACCGAGATCGGTTCCGTGGACTTGCTCGTATGTAAGTGGTGCAGCTCTTTTGGTTAGTCCATGGCCATGCTCCGAGCTCACCAGCACCCAGCAGGATGGTCTGTGCTCTGATAACTTC

Coding sequence (CDS)

ATGCTGCGCTTTTTCCTCTTCCTTTTTTTCTTCTCCGCCGCCGCCGCCGCCGCCGCCGACCTCACTACCTGCTCCGGCATAGTCCCCATGAAGTACCGCAACGACAAGATTTCCATCGAGGACTTTGGGGGAGTTGGCGATGGCCGCACATTGAACACTAGGGCTTTTCGGGAGGCCATTTTTCGGATTCAGCATTTGAAGAGAAGAGGGGGCACTCTGCTTTACATTCCTCCCGGGGTTTTCTTGACTGAGAGCTTCAATCTCACCAGCCATATGACTCTCTATCTCGCTAAAGGCGCTGTTATCAAAGCTGTTCAGGATTCTTCGGATTGGCCCGTAATAGCTCCATTGCCATCTTATGGAAGAGGGAGGGAGCGCCCTGGAGGAAGATATGTGAGCTTTATTCATGGAGATGGCGTTCATGATGTGGTGATCACTGGCGAGAACGGGACTATTGATGGCCAAGGTGATGCTTGGTGGAATATGTGGAAGACAGGGACTCTCAAGTTCACCAGGCCTAATCTCGTTGAGTTTATCAATTCTTATAATATCATCATTTCCAATGTAATTTTCCAGAATTCTCCATTTTGGAACATCCATCCAGTTTACTGCAGAAACGTTGTTGTCAGATATGTCACAATATTGGCTCCTCGTGATTCCCCCAACACCGATGGAATTGATCCAGATTCAAGCAATAACGTTTGCATAGAAGATTCCTACATTTCTACTGGAGATGATCTTGTGGCTGTGAAGAGTGGATGGGATGAATATGGTATCGCTTACGGTCGCAGTAGTTACGACATTACTATCCGAAGAGTGTCAGGGTCAAGCCCATTTGCCGGAGTTGCAGTCGGAAGCGAAACCTCCGGTGGAGTGGCAAACGTATTGGCCGAACACCTGAGTTTTTTTGACATGGGAGTTGGTATTAACATCAAGACAAACATTGGAAGGGGAGGGTTCATAAAGAACATAACTGTGTCCAATGTCTACATGGAGCGTTCGAGAAAGGGTCTAAAGATTGCAGGCGATACCGGGGATCATCCAGACGACAAGTTTGACCCAAATGCTCTTCCGATTGTCAAAGACATCACGATTAAAAATGTTTGGGGCGTGAACGTACAGCAAGCAGGTTCTATATATGGCTTGAAGAACTCTCCATTTACCGGTATATGTCTTTCGAACATCAACCTGCACGGCACAACGAGACCGAGATCGGTTCCGTGGACTTGCTCGTATGTAAGTGGTGCAGCTCTTTTGGTTAGTCCATGGCCATGCTCCGAGCTCACCAGCACCCAGCAGGATGGTCTGTGCTCTGATAACTTC

Protein sequence

MLRFFLFLFFFSAAAAAAADLTTCSGIVPMKYRNDKISIEDFGGVGDGRTLNTRAFREAIFRIQHLKRRGGTLLYIPPGVFLTESFNLTSHMTLYLAKGAVIKAVQDSSDWPVIAPLPSYGRGRERPGGRYVSFIHGDGVHDVVITGENGTIDGQGDAWWNMWKTGTLKFTRPNLVEFINSYNIIISNVIFQNSPFWNIHPVYCRNVVVRYVTILAPRDSPNTDGIDPDSSNNVCIEDSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIRRVSGSSPFAGVAVGSETSGGVANVLAEHLSFFDMGVGINIKTNIGRGGFIKNITVSNVYMERSRKGLKIAGDTGDHPDDKFDPNALPIVKDITIKNVWGVNVQQAGSIYGLKNSPFTGICLSNINLHGTTRPRSVPWTCSYVSGAALLVSPWPCSELTSTQQDGLCSDNF

Homology

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BLAST of MS016282 vs. NCBI nr
Match: XP_022146051.1 (probable polygalacturonase [Momordica charantia])

HSP 1 Score: 855.9 bits (2210), Expect = 1.5e-244
Identity = 412/412 (100.00%), Postives = 412/412 (100.00%), Query Frame = 0


Query: 30  MKYRNDKISIEDFGGVGDGRTLNTRAFREAIFRIQHLKRRGGTLLYIPPGVFLTESFNLT 89
           MKYRNDKISIEDFGGVGDGRTLNTRAFREAIFRIQHLKRRGGTLLYIPPGVFLTESFNLT
Sbjct: 1   MKYRNDKISIEDFGGVGDGRTLNTRAFREAIFRIQHLKRRGGTLLYIPPGVFLTESFNLT 60

Query: 90  SHMTLYLAKGAVIKAVQDSSDWPVIAPLPSYGRGRERPGGRYVSFIHGDGVHDVVITGEN 149
           SHMTLYLAKGAVIKAVQDSSDWPVIAPLPSYGRGRERPGGRYVSFIHGDGVHDVVITGEN
Sbjct: 61  SHMTLYLAKGAVIKAVQDSSDWPVIAPLPSYGRGRERPGGRYVSFIHGDGVHDVVITGEN 120

Query: 150 GTIDGQGDAWWNMWKTGTLKFTRPNLVEFINSYNIIISNVIFQNSPFWNIHPVYCRNVVV 209
           GTIDGQGDAWWNMWKTGTLKFTRPNLVEFINSYNIIISNVIFQNSPFWNIHPVYCRNVVV
Sbjct: 121 GTIDGQGDAWWNMWKTGTLKFTRPNLVEFINSYNIIISNVIFQNSPFWNIHPVYCRNVVV 180

Query: 210 RYVTILAPRDSPNTDGIDPDSSNNVCIEDSYISTGDDLVAVKSGWDEYGIAYGRSSYDIT 269
           RYVTILAPRDSPNTDGIDPDSSNNVCIEDSYISTGDDLVAVKSGWDEYGIAYGRSSYDIT
Sbjct: 181 RYVTILAPRDSPNTDGIDPDSSNNVCIEDSYISTGDDLVAVKSGWDEYGIAYGRSSYDIT 240

Query: 270 IRRVSGSSPFAGVAVGSETSGGVANVLAEHLSFFDMGVGINIKTNIGRGGFIKNITVSNV 329
           IRRVSGSSPFAGVAVGSETSGGVANVLAEHLSFFDMGVGINIKTNIGRGGFIKNITVSNV
Sbjct: 241 IRRVSGSSPFAGVAVGSETSGGVANVLAEHLSFFDMGVGINIKTNIGRGGFIKNITVSNV 300

Query: 330 YMERSRKGLKIAGDTGDHPDDKFDPNALPIVKDITIKNVWGVNVQQAGSIYGLKNSPFTG 389
           YMERSRKGLKIAGDTGDHPDDKFDPNALPIVKDITIKNVWGVNVQQAGSIYGLKNSPFTG
Sbjct: 301 YMERSRKGLKIAGDTGDHPDDKFDPNALPIVKDITIKNVWGVNVQQAGSIYGLKNSPFTG 360

Query: 390 ICLSNINLHGTTRPRSVPWTCSYVSGAALLVSPWPCSELTSTQQDGLCSDNF 442
           ICLSNINLHGTTRPRSVPWTCSYVSGAALLVSPWPCSELTSTQQDGLCSDNF
Sbjct: 361 ICLSNINLHGTTRPRSVPWTCSYVSGAALLVSPWPCSELTSTQQDGLCSDNF 412

[Close]

BLAST of MS016282 vs. NCBI nr
Match: XP_038876129.1 (probable polygalacturonase [Benincasa hispida])

HSP 1 Score: 846.7 bits (2186), Expect = 9.3e-242
Identity = 407/445 (91.46%), Postives = 425/445 (95.51%), Query Frame = 0


Query: 1   MLRFFLFLFFFSA----AAAAAADLTTCSGIVPMKYRNDKISIEDFGGVGDGRTLNTRAF 60
           M+ FFLF+FF+      +  A ADLTTCSGIVPMKYRNDKISI DFGG+GDGRTLNTRAF
Sbjct: 1   MVPFFLFIFFYFTTLFPSPLAVADLTTCSGIVPMKYRNDKISITDFGGIGDGRTLNTRAF 60

Query: 61  REAIFRIQHLKRRGGTLLYIPPGVFLTESFNLTSHMTLYLAKGAVIKAVQDSSDWPVIAP 120
           R AI+RIQHL+RRGGTLLYIPPGV+LTESFNLTSHMTLYLAKGAVI+AVQDSS+WPVIAP
Sbjct: 61  RAAIYRIQHLRRRGGTLLYIPPGVYLTESFNLTSHMTLYLAKGAVIRAVQDSSNWPVIAP 120

Query: 121 LPSYGRGRERPGGRYVSFIHGDGVHDVVITGENGTIDGQGDAWWNMWKTGTLKFTRPNLV 180
           LPSYGRGRERPGGRYVSFIHGDGVHDVVITGENGTIDGQGDAWWNMW+TGTLKFTRPNLV
Sbjct: 121 LPSYGRGRERPGGRYVSFIHGDGVHDVVITGENGTIDGQGDAWWNMWRTGTLKFTRPNLV 180

Query: 181 EFINSYNIIISNVIFQNSPFWNIHPVYCRNVVVRYVTILAPRDSPNTDGIDPDSSNNVCI 240
           EFINSYNIIISNV+F NSPFWNIHPVYC NVVVRYVTILAPRDSPNTDG+DPDSSNNVCI
Sbjct: 181 EFINSYNIIISNVMFLNSPFWNIHPVYCTNVVVRYVTILAPRDSPNTDGVDPDSSNNVCI 240

Query: 241 EDSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIRRVSGSSPFAGVAVGSETSGGVANVL 300
           EDSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIRRVSGSSPFAGVAVGSETSGGV NVL
Sbjct: 241 EDSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIRRVSGSSPFAGVAVGSETSGGVENVL 300

Query: 301 AEHLSFFDMGVGINIKTNIGRGGFIKNITVSNVYMERSRKGLKIAGDTGDHPDDKFDPNA 360
           AEHL+F+DMGVGINIKTNIGRGGFI+NITVSNVYME SRKGLKIAGDTGDHPDDK+DPNA
Sbjct: 301 AEHLNFYDMGVGINIKTNIGRGGFIRNITVSNVYMESSRKGLKIAGDTGDHPDDKYDPNA 360

Query: 361 LPIVKDITIKNVWGVNVQQAGSIYGLKNSPFTGICLSNINLHGTTRPRSVPWTCSYVSGA 420
           LPIVKDITIKNVWG NVQQAGSIYGL+NSPFTGICLSNINL G  RPRSVPWTCSYVSGA
Sbjct: 361 LPIVKDITIKNVWGENVQQAGSIYGLRNSPFTGICLSNINLRGARRPRSVPWTCSYVSGA 420

Query: 421 ALLVSPWPCSELTSTQQDGLCSDNF 442
           ALLVSPWPCSELTSTQQDGLCSDNF
Sbjct: 421 ALLVSPWPCSELTSTQQDGLCSDNF 445

[Close]

BLAST of MS016282 vs. NCBI nr
Match: XP_008437386.1 (PREDICTED: probable polygalacturonase [Cucumis melo] >KAA0042687.1 putative polygalacturonase [Cucumis melo var. makuwa] >TYK06090.1 putative polygalacturonase [Cucumis melo var. makuwa])

HSP 1 Score: 837.4 bits (2162), Expect = 5.6e-239
Identity = 402/445 (90.34%), Postives = 422/445 (94.83%), Query Frame = 0


Query: 1   MLRFFLFLFFFSA----AAAAAADLTTCSGIVPMKYRNDKISIEDFGGVGDGRTLNTRAF 60
           M+ F LF+F F      +  A ADL TCSGIVPMKYRNDKISI DFGG+GDGRTLNTRAF
Sbjct: 1   MVPFSLFVFIFFTTLFPSPLAVADLITCSGIVPMKYRNDKISIADFGGIGDGRTLNTRAF 60

Query: 61  REAIFRIQHLKRRGGTLLYIPPGVFLTESFNLTSHMTLYLAKGAVIKAVQDSSDWPVIAP 120
           R AI+RIQHL+RRGGTLLYIPPGV+LTE+FNLTSHMTLYLAKGAVIKAVQDSS+WPVIAP
Sbjct: 61  RAAIYRIQHLRRRGGTLLYIPPGVYLTETFNLTSHMTLYLAKGAVIKAVQDSSNWPVIAP 120

Query: 121 LPSYGRGRERPGGRYVSFIHGDGVHDVVITGENGTIDGQGDAWWNMWKTGTLKFTRPNLV 180
           LPSYGRGRERPGGRYVSFIHG GVHDVVITGENGTIDGQGDAWWNMW+TGTLK+TRPNLV
Sbjct: 121 LPSYGRGRERPGGRYVSFIHGHGVHDVVITGENGTIDGQGDAWWNMWRTGTLKYTRPNLV 180

Query: 181 EFINSYNIIISNVIFQNSPFWNIHPVYCRNVVVRYVTILAPRDSPNTDGIDPDSSNNVCI 240
           EFINSYNIIISNV+F NSPFWNIHPVYC NVVVRYVTILAPRDSPNTDG+DPDSSNNVCI
Sbjct: 181 EFINSYNIIISNVMFLNSPFWNIHPVYCSNVVVRYVTILAPRDSPNTDGVDPDSSNNVCI 240

Query: 241 EDSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIRRVSGSSPFAGVAVGSETSGGVANVL 300
           EDSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIRR+SGSSPFAGVAVGSETSGGVANVL
Sbjct: 241 EDSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIRRISGSSPFAGVAVGSETSGGVANVL 300

Query: 301 AEHLSFFDMGVGINIKTNIGRGGFIKNITVSNVYMERSRKGLKIAGDTGDHPDDKFDPNA 360
           AEHL+F+DMGVGINIKTNIGRGGFIKNITVSNVYME SRKGLKIAGDTGDHPDDKFDPNA
Sbjct: 301 AEHLNFYDMGVGINIKTNIGRGGFIKNITVSNVYMENSRKGLKIAGDTGDHPDDKFDPNA 360

Query: 361 LPIVKDITIKNVWGVNVQQAGSIYGLKNSPFTGICLSNINLHGTTRPRSVPWTCSYVSGA 420
           LPIVKDITIKN WGV VQQAGSIYGL++SPFTGICLSNIN  GTTRPRSVPWTCSYVSGA
Sbjct: 361 LPIVKDITIKNAWGVKVQQAGSIYGLRDSPFTGICLSNINFRGTTRPRSVPWTCSYVSGA 420

Query: 421 ALLVSPWPCSELTSTQQDGLCSDNF 442
           AL+VSPWPCSELTST+QDGLCSDNF
Sbjct: 421 ALMVSPWPCSELTSTEQDGLCSDNF 445

[Close]

BLAST of MS016282 vs. NCBI nr
Match: XP_004143871.1 (probable polygalacturonase [Cucumis sativus] >KGN50025.1 hypothetical protein Csa_000391 [Cucumis sativus])

HSP 1 Score: 835.5 bits (2157), Expect = 2.1e-238
Identity = 400/445 (89.89%), Postives = 421/445 (94.61%), Query Frame = 0


Query: 1   MLRFFLFLFFFSA----AAAAAADLTTCSGIVPMKYRNDKISIEDFGGVGDGRTLNTRAF 60
           ML F LF+F F      +  A ADLTTCSGIVPMKYRNDKISI DFGG+GDGRTLNTRAF
Sbjct: 1   MLPFPLFIFIFFTTLFPSPLAVADLTTCSGIVPMKYRNDKISIADFGGIGDGRTLNTRAF 60

Query: 61  REAIFRIQHLKRRGGTLLYIPPGVFLTESFNLTSHMTLYLAKGAVIKAVQDSSDWPVIAP 120
           R AI+RIQHL+RRGGTLLYIPPGV+LTE+FNLTSHMTLYLAKGAVIKAVQDSS+WPVIAP
Sbjct: 61  RAAIYRIQHLRRRGGTLLYIPPGVYLTETFNLTSHMTLYLAKGAVIKAVQDSSNWPVIAP 120

Query: 121 LPSYGRGRERPGGRYVSFIHGDGVHDVVITGENGTIDGQGDAWWNMWKTGTLKFTRPNLV 180
           LPSYGRGRERPGGRY+S IHGDGVHDVVITGENGTIDGQGDAWWNMW+TGTLK+TRP+LV
Sbjct: 121 LPSYGRGRERPGGRYISLIHGDGVHDVVITGENGTIDGQGDAWWNMWRTGTLKYTRPSLV 180

Query: 181 EFINSYNIIISNVIFQNSPFWNIHPVYCRNVVVRYVTILAPRDSPNTDGIDPDSSNNVCI 240
           EF+NSYNIIISNV+F NSPFWNIHPVYCRNVVVRYVTILAPRDSPNTDG+DPDSSNNVCI
Sbjct: 181 EFVNSYNIIISNVMFLNSPFWNIHPVYCRNVVVRYVTILAPRDSPNTDGVDPDSSNNVCI 240

Query: 241 EDSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIRRVSGSSPFAGVAVGSETSGGVANVL 300
           EDSYISTGDDLVAVKSGWDEYGIAYGR SYDITIRR+SGSSPFAGVAVGSE SGGVANVL
Sbjct: 241 EDSYISTGDDLVAVKSGWDEYGIAYGRCSYDITIRRISGSSPFAGVAVGSEASGGVANVL 300

Query: 301 AEHLSFFDMGVGINIKTNIGRGGFIKNITVSNVYMERSRKGLKIAGDTGDHPDDKFDPNA 360
           AEHL+F+DMGVGINIKTNIGRGGFIKNITVSNVYME SRKGLKIAGD GDHPDDKFDPNA
Sbjct: 301 AEHLNFYDMGVGINIKTNIGRGGFIKNITVSNVYMENSRKGLKIAGDAGDHPDDKFDPNA 360

Query: 361 LPIVKDITIKNVWGVNVQQAGSIYGLKNSPFTGICLSNINLHGTTRPRSVPWTCSYVSGA 420
           LPIVKDITIKN+WGVNVQQAGSIYGL++SPFTGICLSNINL G TRPRSVPWTCSYVSGA
Sbjct: 361 LPIVKDITIKNIWGVNVQQAGSIYGLRDSPFTGICLSNINLRGATRPRSVPWTCSYVSGA 420

Query: 421 ALLVSPWPCSELTSTQQDGLCSDNF 442
           A LVSPWPCSELTST QDGLCSDNF
Sbjct: 421 ASLVSPWPCSELTSTDQDGLCSDNF 445

[Close]

BLAST of MS016282 vs. NCBI nr
Match: XP_022970040.1 (probable polygalacturonase [Cucurbita maxima] >XP_022970041.1 probable polygalacturonase [Cucurbita maxima])

HSP 1 Score: 830.5 bits (2144), Expect = 6.9e-237
Identity = 403/444 (90.77%), Postives = 420/444 (94.59%), Query Frame = 0


Query: 1   MLRFFLFLFFFSA----AAAAAADLTTCSGIVPMKYRNDKISIEDFGGVGDGRTLNTRAF 60
           ML F LF+F + A    +  A A LTTCSGIVPM YRNDKISI DFGG+GDGRTLNT+AF
Sbjct: 1   MLPFSLFIFIYFATLLPSPFAIAQLTTCSGIVPMNYRNDKISITDFGGIGDGRTLNTKAF 60

Query: 61  REAIFRIQHLKRRGGTLLYIPPGVFLTESFNLTSHMTLYLAKGAVIKAVQDSSDWPVIAP 120
           R AI+RIQ+LKRRGGTLLYIPPGV+LTESFNLTSHMTLYLAK AVIKAVQ SS+WPV+AP
Sbjct: 61  RAAIYRIQNLKRRGGTLLYIPPGVYLTESFNLTSHMTLYLAKDAVIKAVQGSSNWPVVAP 120

Query: 121 LPSYGRGRERPGGRYVSFIHGDGVHDVVITGENGTIDGQGDAWWNMWKTGTLKFTRPNLV 180
           LPSYGRGRERPGGRYVSFIHGDGVHDVVITGENGTIDGQGDAWWNMW+TGTLKFTRPNLV
Sbjct: 121 LPSYGRGRERPGGRYVSFIHGDGVHDVVITGENGTIDGQGDAWWNMWRTGTLKFTRPNLV 180

Query: 181 EFINSYNIIISNVIFQNSPFWNIHPVYCRNVVVRYVTILAPRDSPNTDGIDPDSSNNVCI 240
           EFINSYNIIISNV+F NSPFWNIHPVYC NVVVRYVTILAPRDSPNTDGIDPDSSNNVCI
Sbjct: 181 EFINSYNIIISNVMFLNSPFWNIHPVYCSNVVVRYVTILAPRDSPNTDGIDPDSSNNVCI 240

Query: 241 EDSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIRRVSGSSPFAGVAVGSETSGGVANVL 300
           EDSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIRRVSGSSPFAGVAVGSETSGGVANVL
Sbjct: 241 EDSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIRRVSGSSPFAGVAVGSETSGGVANVL 300

Query: 301 AEHLSFFDMGVGINIKTNIGRGGFIKNITVSNVYMERSRKGLKIAGDTGDHPDDKFDPNA 360
           AEHL+F+DMG+GINIKTNIGRGGFIKNITVSNVYMERSRKGLKIAGDTGDHPDDKFDPNA
Sbjct: 301 AEHLNFYDMGIGINIKTNIGRGGFIKNITVSNVYMERSRKGLKIAGDTGDHPDDKFDPNA 360

Query: 361 LPIVKDITIKNVWGVNVQQAGSIYGLKNSPFTGICLSNINLHGTTRPRSVPWTCSYVSGA 420
           LPIVKDITIKNVWGVNVQQAGSIYGL+NSPFTGICLSNI+L G  RPRSVPWTCSYVSGA
Sbjct: 361 LPIVKDITIKNVWGVNVQQAGSIYGLRNSPFTGICLSNIHLRGAMRPRSVPWTCSYVSGA 420

Query: 421 ALLVSPWPCSELTSTQQDGLCSDN 441
           ALLVSPWPCSELTSTQQDG CS N
Sbjct: 421 ALLVSPWPCSELTSTQQDGSCSAN 444

[Close]

BLAST of MS016282 vs. ExPASy Swiss-Prot
Match: A7PZL3 (Probable polygalacturonase OS=Vitis vinifera OX=29760 GN=GSVIVT00026920001 PE=1 SV=1)

HSP 1 Score: 421.8 bits (1083), Expect = 9.7e-117
Identity = 204/409 (49.88%), Postives = 276/409 (67.48%), Query Frame = 0


Query: 33  RNDKISIEDFGGVGDGRTLNTRAFREAIFRIQHLKRRGGTLLYIPPGVFLTESFNLTSHM 92
           R    S+ DFGGVGDG+TLNT+AF++A+  +      GG  LY+P G +LT SF+LTSH 
Sbjct: 59  RAHSASLVDFGGVGDGQTLNTKAFQDAVSELSKYGSEGGAQLYVPAGKWLTGSFSLTSHF 118

Query: 93  TLYLAKGAVIKAVQDSSDWPVIAPLPSYGRGRERPGGRYVSFIHGDGVHDVVITGENGTI 152
           TL+L + AV+ A QD S WPVI PLPSYGRGR+   GRY S I G  + DV+ITG+NGTI
Sbjct: 119 TLFLHRDAVLLASQDISQWPVIKPLPSYGRGRDAAAGRYTSLIFGTNLTDVIITGDNGTI 178

Query: 153 DGQGDAWWNMWKTGTLKFTRPNLVEFINSYNIIISNVIFQNSPFWNIHPVYCRNVVVRYV 212
           DGQG  WW  +  G LK+TRP L+E + S +I ISN+   NSP WN+HPVY RN++++ +
Sbjct: 179 DGQGGLWWQRFHGGKLKYTRPYLIELMYSADIQISNLTLLNSPSWNVHPVYSRNILIQGI 238

Query: 213 TILAPRDSPNTDGIDPDSSNNVCIEDSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIRR 272
           TILAP  SPNTDGI+PDS  N  IED YI +GDD VAVKSGWDEYGIAYG  +  + IRR
Sbjct: 239 TILAPVRSPNTDGINPDSCTNTRIEDCYIVSGDDCVAVKSGWDEYGIAYGMPTKQLVIRR 298

Query: 273 VSGSSPFAGV-AVGSETSGGVANVLAEHLSFFDMGVGINIKTNIGRGGFIKNITVSNVYM 332
           ++  SP++ V A+GSE SGG+ +V AE +   +   GI IKT IGRGG++K+I V  + M
Sbjct: 299 LTCISPYSAVIALGSEMSGGIQDVRAEDIVAINSESGIRIKTGIGRGGYVKDIYVRGMTM 358

Query: 333 ERSRKGLKIAGDTGDHPDDKFDPNALPIVKDITIKNVWGVNVQQAGSIYGLKNSPFTGIC 392
           +  +    + G+ G H D+ +DP A P+++ I  +++   NV  A  + G+ + PFTGIC
Sbjct: 359 KTMKWAFWMTGNYGSHADNHYDPKAFPVIQGINYRDMVAENVSMAARLEGIPSDPFTGIC 418

Query: 393 LSNINLHGTTRPRSVPWTCSYVSGAALLVSPWPCSEL--TSTQQDGLCS 439
           +SN+ +H   + + VPWTC+ V G +  V+P PCS L     ++  LC+
Sbjct: 419 ISNVTIHLAAKAKKVPWTCTDVEGISSGVTPTPCSTLPDQGPEKTSLCN 467

[Close]

BLAST of MS016282 vs. ExPASy Swiss-Prot
Match: Q8RY29 (Polygalacturonase ADPG2 OS=Arabidopsis thaliana OX=3702 GN=ADPG2 PE=2 SV=2)

HSP 1 Score: 134.0 bits (336), Expect = 4.0e-30
Identity = 105/375 (28.00%), Postives = 174/375 (46.40%), Query Frame = 0


Query: 37  ISIEDFGGVGDGRTLNTRAFREAIFRIQHLKRRGGTLLYIPPG-VFLTESFNLTS--HMT 96
           +S+ DFG  GDG+T +T+AF  A  +       G   L +P G  +L +S  LT   +  
Sbjct: 68  VSVSDFGAKGDGKTDDTQAFVNAWKKA--CSSNGAVNLLVPKGNTYLLKSIQLTGPCNSI 127

Query: 97  LYLAKGAVIKAVQDSSDWPVIAPLPSYGRGRERPGGRYVSFIHGDGVHDVVIT-GENGTI 156
           L +     + A Q  SD+  I+              +++ F   DGV+++ +  G+ G +
Sbjct: 128 LTVQIFGTLSASQKRSDYKDIS--------------KWIMF---DGVNNLSVDGGDTGVV 187

Query: 157 DGQGDAWW-NMWKTGTLK--FTRPNLVEFINSYNIIISNVIFQNSPFWNIHPVYCRNVVV 216
           DG G+ WW N  K    K     P  + F NS ++I+ N+  +N+    I    C NV V
Sbjct: 188 DGNGETWWQNSCKRNKAKPCTKAPTALTFYNSKSLIVKNLKVRNAQQIQISIEKCSNVQV 247

Query: 217 RYVTILAPRDSPNTDGIDPDSSNNVCIEDSYISTGDDLVAVKSGWDEYGIAYGRSSYDIT 276
             V + AP DSPNTDGI   ++ N+ + +S I TGDD ++++SG           S ++ 
Sbjct: 248 SNVVVTAPADSPNTDGIHITNTQNIRVSESIIGTGDDCISIESG-----------SQNVQ 307

Query: 277 IRRVSGSSPFAGVAVGS----ETSGGVANVLAEHLSFFDMGVGINIKTNIGRGGFIKNIT 336
           I  ++   P  G+++GS     +   V+ V  +         G+ IKT  G  G   NI 
Sbjct: 308 INDIT-CGPGHGISIGSLGDDNSKAFVSGVTVDGAKLSGTDNGVRIKTYQGGSGTASNII 367

Query: 337 VSNVYMERSRKGLKIAGDTGDHPDDKFDPNALPIVKDITIKNVWGVNVQQAGSIYGL-KN 396
             N+ M+  +  + I  D  D      + +A+  VK++  +++ G +  +    +   KN
Sbjct: 368 FQNIQMDNVKNPIIIDQDYCDKSKCTTEKSAVQ-VKNVVYRDISGTSASENAITFNCSKN 410

Query: 397 SPFTGICLSNINLHG 400
            P  GI L  +N+ G
Sbjct: 428 YPCQGIVLDRVNIKG 410

[Close]

BLAST of MS016282 vs. ExPASy Swiss-Prot
Match: O23147 (Polygalacturonase ADPG1 OS=Arabidopsis thaliana OX=3702 GN=ADPG1 PE=2 SV=1)

HSP 1 Score: 111.3 bits (277), Expect = 2.8e-23
Identity = 99/375 (26.40%), Postives = 164/375 (43.73%), Query Frame = 0


Query: 37  ISIEDFGGVGDGRTLNTRAFREAIFRIQHLKRRGGTLLYIPPGVFLTESFNLTSHMTLYL 96
           +S+ +FG  GDG+T +T+AF++A  +       G T   +P G               YL
Sbjct: 68  VSVSNFGAKGDGKTDDTQAFKKAWKKA--CSTNGVTTFLVPKG-------------KTYL 127

Query: 97  AKGAVIKA-VQDSSDWPVIAPLPSYGRGRERPGGRYVSFIHGDGVHDVVIT-GENGTIDG 156
            K    +   +   ++ ++  L +  +  +     +   +  + V+++ I  G  G I+G
Sbjct: 128 LKSTRFRGPCKSLRNFQILGTLSASTKRSDYKDKNHWLIL--EDVNNLSIDGGSTGIING 187

Query: 157 QGDAWW-NMWKTGTLK--FTRPNLVEFINSYNIIISNVIFQNSPFWNIHPVYCRNVVVRY 216
            G  WW N  K    K     P  +   N  N+ + N+  +N+    I    C  V V  
Sbjct: 188 NGKTWWQNSCKIDKSKPCTKAPTALTLYNLKNLNVKNLRVKNAQQIQISIEKCNKVEVSN 247

Query: 217 VTILAPRDSPNTDGIDPDSSNNVCIEDSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIR 276
           V I AP DSPNTDGI   ++ N+ + +S I TGDD ++++ G     I      +D+T  
Sbjct: 248 VEITAPGDSPNTDGIHITNTQNIRVSNSDIGTGDDCISIEDGTQNLQI------FDLT-- 307

Query: 277 RVSGSSPFAGVAVGS----ETSGGVANVLAEHLSFFDMGVGINIKTNIGRGGFIKNITVS 336
                 P  G+++GS     +   V+ +  +   F +   G+ IKT  G  G  KNI   
Sbjct: 308 ----CGPGHGISIGSLGDDNSKAYVSGINVDGAKFSESDNGVRIKTYQGGSGTAKNIKFQ 367

Query: 337 NVYMERSRKGLKIAGDTGDHPDDKFDPNALPIVKDITIKNVWGVNVQQ-AGSIYGLKNSP 396
           N+ ME  +  + I  D  D  D   D  +   VK++  KN+ G +    A ++   +  P
Sbjct: 368 NIRMENVKNPIIIDQDYCD-KDKCEDQESAVQVKNVVYKNISGTSATDVAITLNCSEKYP 412

Query: 397 FTGICLSNINLHGTT 402
             GI L N+ + G T
Sbjct: 428 CQGIVLENVKIKGGT 412

[Close]

BLAST of MS016282 vs. ExPASy Swiss-Prot
Match: Q02096 (Polygalacturonase OS=Persea americana OX=3435 PE=2 SV=1)

HSP 1 Score: 111.3 bits (277), Expect = 2.8e-23
Identity = 99/374 (26.47%), Postives = 166/374 (44.39%), Query Frame = 0


Query: 37  ISIEDFGGVGDGRTLNTRAFREAIFRIQHLKRRGGTLLYIPPG---VFLTESFNLTSHMT 96
           IS++DFG  GDG T +T+AF +A           G++L +P     +    +F+      
Sbjct: 85  ISVDDFGARGDG-TDDTKAFEKA----WKDACSSGSVLIVPENKNYLLKQITFSGPCKSD 144

Query: 97  LYLAKGAVIKAVQDSSDWPVIAPLPSYGRGRERPGGRYVSFIHGDGVHDVVITGENGTID 156
           L +     I+A  D SDW         G  R+R       +I  + + ++ + G  GT +
Sbjct: 145 LRVKIRGTIEASSDQSDW--------VGHNRKR-------WIEFEDISNLTLEG-GGTSN 204

Query: 157 GQGDAWWN---MWKTGTLKFTRPNLVEFINSYNIIISNVIFQNSPFWNIHPVYCRNVVVR 216
           G G+ WW+     K      + P  + F +  N+I+S++  ++S   ++    C++V+  
Sbjct: 205 GNGETWWDSSCKRKKSLPCKSAPTALTFRSCKNLIVSDLSIKDSQKMHLSFDKCQDVIAS 264

Query: 217 YVTILAPRDSPNTDGIDPDSSNNVCIEDSYISTGDDLVAVKSGWDEYGIAYGRSSYDITI 276
            + + AP  SPNTDGI    +  + + +S I TGDD ++++SG          S   I  
Sbjct: 265 NLMVTAPEHSPNTDGIHITGTQRIHVMNSVIGTGDDCISIESG----------SKMVIAT 324

Query: 277 RRVSGSSPFAGVAVGS----ETSGGVANVLAEHLSFFDMGVGINIKTNIGRGGFIKNITV 336
               G  P  G+++GS     +   V+ VL +  + FD   G+ IKT  G  G  KNI  
Sbjct: 325 NITCG--PGHGISIGSLGDRNSEAHVSGVLVDGGNLFDTTNGLRIKTWQGGSGSAKNIKF 384

Query: 337 SNVYMERSRKGLKIAGDTGDHPDDKFDPNALPIVKDITIKNVWGVNVQQAGSIYGL-KNS 396
            N+ M      + I     D  D   +  +   V ++   N+ G +  +    +   K+S
Sbjct: 385 QNIVMHNVTNPIIIDQYYCDSKDPCPEQESAVKVSNVAYMNIRGTSASEVAVKFDCSKSS 425

Query: 397 PFTGICLSNINLHG 400
           P  G  + NINL G
Sbjct: 445 PCQGYIVGNINLVG 425

[Close]

BLAST of MS016282 vs. ExPASy Swiss-Prot
Match: Q7M1E7 (Polygalacturonase OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1)

HSP 1 Score: 102.4 bits (254), Expect = 1.3e-20
Identity = 101/376 (26.86%), Postives = 160/376 (42.55%), Query Frame = 0


Query: 38  SIEDFGGVGDGRTLNTRAFREAIFRIQHLKRRGGTLLYIPPGVFLTESFNLTSHMTLYLA 97
           ++E +G VGDG+  +T AF           ++   +L +P         N    +   + 
Sbjct: 60  NVEQYGAVGDGKHDSTEAFATT---WNAACKKASAVLLVPA--------NKKFFVNNLVF 119

Query: 98  KGAVIKAVQDSSDWPVIA-PLPSYGRGRERPGGRYVSFIHGDGVHDVVITGENGTIDGQG 157
           +G     +    D  ++A P P+    R +    ++ F     + D  + G  G IDGQG
Sbjct: 120 RGPCQPHLSFKVDGTIVAQPDPA----RWKNSKIWLQFAQ---LTDFNLMG-TGVIDGQG 179

Query: 158 DAWWN------MWKTGTLKFTRPNLVEFINSYNIIISNVIFQNSPFWNIHPVYCRNVVVR 217
             WW         +T      RP  ++   S ++ +  +   NSP +++    C  V ++
Sbjct: 180 QQWWAGQCKVVNGRTVCNDRNRPTAIKIDYSKSVTVKELTLMNSPEFHLVFGECEGVKIQ 239

Query: 218 YVTILAPRDSPNTDGIDPDSSNNVCIEDSYISTGDDLVAVKSGWDEYGIAYGRSSYDITI 277
            + I APRDSPNTDGID  +S    IE   I TGDD +A+           G  S +ITI
Sbjct: 240 GLKIKAPRDSPNTDGIDIFASKRFHIEKCVIGTGDDCIAI-----------GTGSSNITI 299

Query: 278 RRVSGSSPFAGVAVGS----ETSGGVANVLAEHLSFFDMGVGINIKTNIGRGGFIKNITV 337
           + +    P  G+++GS     +   V++V      F D   G+ IKT  G  G    IT 
Sbjct: 300 KDLI-CGPGHGISIGSLGRDNSRAEVSHVHVNRAKFIDTQNGLRIKTWQGGSGLASYITY 359

Query: 338 SNVYMERSRKGLKIAGDTGDHPDDKFDPNALPIVKDITIKNVWGVNVQQAGSIYGLKNS- 397
            NV M  S   + I            +  +   ++ +T KN+ G +   A       +S 
Sbjct: 360 ENVEMINSENPILINQFYCTSASACQNQRSAVQIQGVTYKNIHGTSATAAAIQLMCSDSV 404

Query: 398 PFTGICLSNINLHGTT 402
           P TGI LSN++L  T+
Sbjct: 420 PCTGIQLSNVSLKLTS 404

[Close]

BLAST of MS016282 vs. ExPASy TrEMBL
Match: A0A6J1CY77 (probable polygalacturonase OS=Momordica charantia OX=3673 GN=LOC111015353 PE=3 SV=1)

HSP 1 Score: 855.9 bits (2210), Expect = 7.4e-245
Identity = 412/412 (100.00%), Postives = 412/412 (100.00%), Query Frame = 0


Query: 30  MKYRNDKISIEDFGGVGDGRTLNTRAFREAIFRIQHLKRRGGTLLYIPPGVFLTESFNLT 89
           MKYRNDKISIEDFGGVGDGRTLNTRAFREAIFRIQHLKRRGGTLLYIPPGVFLTESFNLT
Sbjct: 1   MKYRNDKISIEDFGGVGDGRTLNTRAFREAIFRIQHLKRRGGTLLYIPPGVFLTESFNLT 60

Query: 90  SHMTLYLAKGAVIKAVQDSSDWPVIAPLPSYGRGRERPGGRYVSFIHGDGVHDVVITGEN 149
           SHMTLYLAKGAVIKAVQDSSDWPVIAPLPSYGRGRERPGGRYVSFIHGDGVHDVVITGEN
Sbjct: 61  SHMTLYLAKGAVIKAVQDSSDWPVIAPLPSYGRGRERPGGRYVSFIHGDGVHDVVITGEN 120

Query: 150 GTIDGQGDAWWNMWKTGTLKFTRPNLVEFINSYNIIISNVIFQNSPFWNIHPVYCRNVVV 209
           GTIDGQGDAWWNMWKTGTLKFTRPNLVEFINSYNIIISNVIFQNSPFWNIHPVYCRNVVV
Sbjct: 121 GTIDGQGDAWWNMWKTGTLKFTRPNLVEFINSYNIIISNVIFQNSPFWNIHPVYCRNVVV 180

Query: 210 RYVTILAPRDSPNTDGIDPDSSNNVCIEDSYISTGDDLVAVKSGWDEYGIAYGRSSYDIT 269
           RYVTILAPRDSPNTDGIDPDSSNNVCIEDSYISTGDDLVAVKSGWDEYGIAYGRSSYDIT
Sbjct: 181 RYVTILAPRDSPNTDGIDPDSSNNVCIEDSYISTGDDLVAVKSGWDEYGIAYGRSSYDIT 240

Query: 270 IRRVSGSSPFAGVAVGSETSGGVANVLAEHLSFFDMGVGINIKTNIGRGGFIKNITVSNV 329
           IRRVSGSSPFAGVAVGSETSGGVANVLAEHLSFFDMGVGINIKTNIGRGGFIKNITVSNV
Sbjct: 241 IRRVSGSSPFAGVAVGSETSGGVANVLAEHLSFFDMGVGINIKTNIGRGGFIKNITVSNV 300

Query: 330 YMERSRKGLKIAGDTGDHPDDKFDPNALPIVKDITIKNVWGVNVQQAGSIYGLKNSPFTG 389
           YMERSRKGLKIAGDTGDHPDDKFDPNALPIVKDITIKNVWGVNVQQAGSIYGLKNSPFTG
Sbjct: 301 YMERSRKGLKIAGDTGDHPDDKFDPNALPIVKDITIKNVWGVNVQQAGSIYGLKNSPFTG 360

Query: 390 ICLSNINLHGTTRPRSVPWTCSYVSGAALLVSPWPCSELTSTQQDGLCSDNF 442
           ICLSNINLHGTTRPRSVPWTCSYVSGAALLVSPWPCSELTSTQQDGLCSDNF
Sbjct: 361 ICLSNINLHGTTRPRSVPWTCSYVSGAALLVSPWPCSELTSTQQDGLCSDNF 412

[Close]

BLAST of MS016282 vs. ExPASy TrEMBL
Match: A0A5A7TLS6 (Putative polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold376G001910 PE=3 SV=1)

HSP 1 Score: 837.4 bits (2162), Expect = 2.7e-239
Identity = 402/445 (90.34%), Postives = 422/445 (94.83%), Query Frame = 0


Query: 1   MLRFFLFLFFFSA----AAAAAADLTTCSGIVPMKYRNDKISIEDFGGVGDGRTLNTRAF 60
           M+ F LF+F F      +  A ADL TCSGIVPMKYRNDKISI DFGG+GDGRTLNTRAF
Sbjct: 1   MVPFSLFVFIFFTTLFPSPLAVADLITCSGIVPMKYRNDKISIADFGGIGDGRTLNTRAF 60

Query: 61  REAIFRIQHLKRRGGTLLYIPPGVFLTESFNLTSHMTLYLAKGAVIKAVQDSSDWPVIAP 120
           R AI+RIQHL+RRGGTLLYIPPGV+LTE+FNLTSHMTLYLAKGAVIKAVQDSS+WPVIAP
Sbjct: 61  RAAIYRIQHLRRRGGTLLYIPPGVYLTETFNLTSHMTLYLAKGAVIKAVQDSSNWPVIAP 120

Query: 121 LPSYGRGRERPGGRYVSFIHGDGVHDVVITGENGTIDGQGDAWWNMWKTGTLKFTRPNLV 180
           LPSYGRGRERPGGRYVSFIHG GVHDVVITGENGTIDGQGDAWWNMW+TGTLK+TRPNLV
Sbjct: 121 LPSYGRGRERPGGRYVSFIHGHGVHDVVITGENGTIDGQGDAWWNMWRTGTLKYTRPNLV 180

Query: 181 EFINSYNIIISNVIFQNSPFWNIHPVYCRNVVVRYVTILAPRDSPNTDGIDPDSSNNVCI 240
           EFINSYNIIISNV+F NSPFWNIHPVYC NVVVRYVTILAPRDSPNTDG+DPDSSNNVCI
Sbjct: 181 EFINSYNIIISNVMFLNSPFWNIHPVYCSNVVVRYVTILAPRDSPNTDGVDPDSSNNVCI 240

Query: 241 EDSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIRRVSGSSPFAGVAVGSETSGGVANVL 300
           EDSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIRR+SGSSPFAGVAVGSETSGGVANVL
Sbjct: 241 EDSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIRRISGSSPFAGVAVGSETSGGVANVL 300

Query: 301 AEHLSFFDMGVGINIKTNIGRGGFIKNITVSNVYMERSRKGLKIAGDTGDHPDDKFDPNA 360
           AEHL+F+DMGVGINIKTNIGRGGFIKNITVSNVYME SRKGLKIAGDTGDHPDDKFDPNA
Sbjct: 301 AEHLNFYDMGVGINIKTNIGRGGFIKNITVSNVYMENSRKGLKIAGDTGDHPDDKFDPNA 360

Query: 361 LPIVKDITIKNVWGVNVQQAGSIYGLKNSPFTGICLSNINLHGTTRPRSVPWTCSYVSGA 420
           LPIVKDITIKN WGV VQQAGSIYGL++SPFTGICLSNIN  GTTRPRSVPWTCSYVSGA
Sbjct: 361 LPIVKDITIKNAWGVKVQQAGSIYGLRDSPFTGICLSNINFRGTTRPRSVPWTCSYVSGA 420

Query: 421 ALLVSPWPCSELTSTQQDGLCSDNF 442
           AL+VSPWPCSELTST+QDGLCSDNF
Sbjct: 421 ALMVSPWPCSELTSTEQDGLCSDNF 445

[Close]

BLAST of MS016282 vs. ExPASy TrEMBL
Match: A0A1S3ATJ9 (probable polygalacturonase OS=Cucumis melo OX=3656 GN=LOC103482817 PE=3 SV=1)

HSP 1 Score: 837.4 bits (2162), Expect = 2.7e-239
Identity = 402/445 (90.34%), Postives = 422/445 (94.83%), Query Frame = 0


Query: 1   MLRFFLFLFFFSA----AAAAAADLTTCSGIVPMKYRNDKISIEDFGGVGDGRTLNTRAF 60
           M+ F LF+F F      +  A ADL TCSGIVPMKYRNDKISI DFGG+GDGRTLNTRAF
Sbjct: 1   MVPFSLFVFIFFTTLFPSPLAVADLITCSGIVPMKYRNDKISIADFGGIGDGRTLNTRAF 60

Query: 61  REAIFRIQHLKRRGGTLLYIPPGVFLTESFNLTSHMTLYLAKGAVIKAVQDSSDWPVIAP 120
           R AI+RIQHL+RRGGTLLYIPPGV+LTE+FNLTSHMTLYLAKGAVIKAVQDSS+WPVIAP
Sbjct: 61  RAAIYRIQHLRRRGGTLLYIPPGVYLTETFNLTSHMTLYLAKGAVIKAVQDSSNWPVIAP 120

Query: 121 LPSYGRGRERPGGRYVSFIHGDGVHDVVITGENGTIDGQGDAWWNMWKTGTLKFTRPNLV 180
           LPSYGRGRERPGGRYVSFIHG GVHDVVITGENGTIDGQGDAWWNMW+TGTLK+TRPNLV
Sbjct: 121 LPSYGRGRERPGGRYVSFIHGHGVHDVVITGENGTIDGQGDAWWNMWRTGTLKYTRPNLV 180

Query: 181 EFINSYNIIISNVIFQNSPFWNIHPVYCRNVVVRYVTILAPRDSPNTDGIDPDSSNNVCI 240
           EFINSYNIIISNV+F NSPFWNIHPVYC NVVVRYVTILAPRDSPNTDG+DPDSSNNVCI
Sbjct: 181 EFINSYNIIISNVMFLNSPFWNIHPVYCSNVVVRYVTILAPRDSPNTDGVDPDSSNNVCI 240

Query: 241 EDSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIRRVSGSSPFAGVAVGSETSGGVANVL 300
           EDSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIRR+SGSSPFAGVAVGSETSGGVANVL
Sbjct: 241 EDSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIRRISGSSPFAGVAVGSETSGGVANVL 300

Query: 301 AEHLSFFDMGVGINIKTNIGRGGFIKNITVSNVYMERSRKGLKIAGDTGDHPDDKFDPNA 360
           AEHL+F+DMGVGINIKTNIGRGGFIKNITVSNVYME SRKGLKIAGDTGDHPDDKFDPNA
Sbjct: 301 AEHLNFYDMGVGINIKTNIGRGGFIKNITVSNVYMENSRKGLKIAGDTGDHPDDKFDPNA 360

Query: 361 LPIVKDITIKNVWGVNVQQAGSIYGLKNSPFTGICLSNINLHGTTRPRSVPWTCSYVSGA 420
           LPIVKDITIKN WGV VQQAGSIYGL++SPFTGICLSNIN  GTTRPRSVPWTCSYVSGA
Sbjct: 361 LPIVKDITIKNAWGVKVQQAGSIYGLRDSPFTGICLSNINFRGTTRPRSVPWTCSYVSGA 420

Query: 421 ALLVSPWPCSELTSTQQDGLCSDNF 442
           AL+VSPWPCSELTST+QDGLCSDNF
Sbjct: 421 ALMVSPWPCSELTSTEQDGLCSDNF 445

[Close]

BLAST of MS016282 vs. ExPASy TrEMBL
Match: A0A0A0KK36 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G150430 PE=3 SV=1)

HSP 1 Score: 835.5 bits (2157), Expect = 1.0e-238
Identity = 400/445 (89.89%), Postives = 421/445 (94.61%), Query Frame = 0


Query: 1   MLRFFLFLFFFSA----AAAAAADLTTCSGIVPMKYRNDKISIEDFGGVGDGRTLNTRAF 60
           ML F LF+F F      +  A ADLTTCSGIVPMKYRNDKISI DFGG+GDGRTLNTRAF
Sbjct: 1   MLPFPLFIFIFFTTLFPSPLAVADLTTCSGIVPMKYRNDKISIADFGGIGDGRTLNTRAF 60

Query: 61  REAIFRIQHLKRRGGTLLYIPPGVFLTESFNLTSHMTLYLAKGAVIKAVQDSSDWPVIAP 120
           R AI+RIQHL+RRGGTLLYIPPGV+LTE+FNLTSHMTLYLAKGAVIKAVQDSS+WPVIAP
Sbjct: 61  RAAIYRIQHLRRRGGTLLYIPPGVYLTETFNLTSHMTLYLAKGAVIKAVQDSSNWPVIAP 120

Query: 121 LPSYGRGRERPGGRYVSFIHGDGVHDVVITGENGTIDGQGDAWWNMWKTGTLKFTRPNLV 180
           LPSYGRGRERPGGRY+S IHGDGVHDVVITGENGTIDGQGDAWWNMW+TGTLK+TRP+LV
Sbjct: 121 LPSYGRGRERPGGRYISLIHGDGVHDVVITGENGTIDGQGDAWWNMWRTGTLKYTRPSLV 180

Query: 181 EFINSYNIIISNVIFQNSPFWNIHPVYCRNVVVRYVTILAPRDSPNTDGIDPDSSNNVCI 240
           EF+NSYNIIISNV+F NSPFWNIHPVYCRNVVVRYVTILAPRDSPNTDG+DPDSSNNVCI
Sbjct: 181 EFVNSYNIIISNVMFLNSPFWNIHPVYCRNVVVRYVTILAPRDSPNTDGVDPDSSNNVCI 240

Query: 241 EDSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIRRVSGSSPFAGVAVGSETSGGVANVL 300
           EDSYISTGDDLVAVKSGWDEYGIAYGR SYDITIRR+SGSSPFAGVAVGSE SGGVANVL
Sbjct: 241 EDSYISTGDDLVAVKSGWDEYGIAYGRCSYDITIRRISGSSPFAGVAVGSEASGGVANVL 300

Query: 301 AEHLSFFDMGVGINIKTNIGRGGFIKNITVSNVYMERSRKGLKIAGDTGDHPDDKFDPNA 360
           AEHL+F+DMGVGINIKTNIGRGGFIKNITVSNVYME SRKGLKIAGD GDHPDDKFDPNA
Sbjct: 301 AEHLNFYDMGVGINIKTNIGRGGFIKNITVSNVYMENSRKGLKIAGDAGDHPDDKFDPNA 360

Query: 361 LPIVKDITIKNVWGVNVQQAGSIYGLKNSPFTGICLSNINLHGTTRPRSVPWTCSYVSGA 420
           LPIVKDITIKN+WGVNVQQAGSIYGL++SPFTGICLSNINL G TRPRSVPWTCSYVSGA
Sbjct: 361 LPIVKDITIKNIWGVNVQQAGSIYGLRDSPFTGICLSNINLRGATRPRSVPWTCSYVSGA 420

Query: 421 ALLVSPWPCSELTSTQQDGLCSDNF 442
           A LVSPWPCSELTST QDGLCSDNF
Sbjct: 421 ASLVSPWPCSELTSTDQDGLCSDNF 445

[Close]

BLAST of MS016282 vs. ExPASy TrEMBL
Match: A0A6J1I1Q1 (probable polygalacturonase OS=Cucurbita maxima OX=3661 GN=LOC111469049 PE=3 SV=1)

HSP 1 Score: 830.5 bits (2144), Expect = 3.3e-237
Identity = 403/444 (90.77%), Postives = 420/444 (94.59%), Query Frame = 0


Query: 1   MLRFFLFLFFFSA----AAAAAADLTTCSGIVPMKYRNDKISIEDFGGVGDGRTLNTRAF 60
           ML F LF+F + A    +  A A LTTCSGIVPM YRNDKISI DFGG+GDGRTLNT+AF
Sbjct: 1   MLPFSLFIFIYFATLLPSPFAIAQLTTCSGIVPMNYRNDKISITDFGGIGDGRTLNTKAF 60

Query: 61  REAIFRIQHLKRRGGTLLYIPPGVFLTESFNLTSHMTLYLAKGAVIKAVQDSSDWPVIAP 120
           R AI+RIQ+LKRRGGTLLYIPPGV+LTESFNLTSHMTLYLAK AVIKAVQ SS+WPV+AP
Sbjct: 61  RAAIYRIQNLKRRGGTLLYIPPGVYLTESFNLTSHMTLYLAKDAVIKAVQGSSNWPVVAP 120

Query: 121 LPSYGRGRERPGGRYVSFIHGDGVHDVVITGENGTIDGQGDAWWNMWKTGTLKFTRPNLV 180
           LPSYGRGRERPGGRYVSFIHGDGVHDVVITGENGTIDGQGDAWWNMW+TGTLKFTRPNLV
Sbjct: 121 LPSYGRGRERPGGRYVSFIHGDGVHDVVITGENGTIDGQGDAWWNMWRTGTLKFTRPNLV 180

Query: 181 EFINSYNIIISNVIFQNSPFWNIHPVYCRNVVVRYVTILAPRDSPNTDGIDPDSSNNVCI 240
           EFINSYNIIISNV+F NSPFWNIHPVYC NVVVRYVTILAPRDSPNTDGIDPDSSNNVCI
Sbjct: 181 EFINSYNIIISNVMFLNSPFWNIHPVYCSNVVVRYVTILAPRDSPNTDGIDPDSSNNVCI 240

Query: 241 EDSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIRRVSGSSPFAGVAVGSETSGGVANVL 300
           EDSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIRRVSGSSPFAGVAVGSETSGGVANVL
Sbjct: 241 EDSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIRRVSGSSPFAGVAVGSETSGGVANVL 300

Query: 301 AEHLSFFDMGVGINIKTNIGRGGFIKNITVSNVYMERSRKGLKIAGDTGDHPDDKFDPNA 360
           AEHL+F+DMG+GINIKTNIGRGGFIKNITVSNVYMERSRKGLKIAGDTGDHPDDKFDPNA
Sbjct: 301 AEHLNFYDMGIGINIKTNIGRGGFIKNITVSNVYMERSRKGLKIAGDTGDHPDDKFDPNA 360

Query: 361 LPIVKDITIKNVWGVNVQQAGSIYGLKNSPFTGICLSNINLHGTTRPRSVPWTCSYVSGA 420
           LPIVKDITIKNVWGVNVQQAGSIYGL+NSPFTGICLSNI+L G  RPRSVPWTCSYVSGA
Sbjct: 361 LPIVKDITIKNVWGVNVQQAGSIYGLRNSPFTGICLSNIHLRGAMRPRSVPWTCSYVSGA 420

Query: 421 ALLVSPWPCSELTSTQQDGLCSDN 441
           ALLVSPWPCSELTSTQQDG CS N
Sbjct: 421 ALLVSPWPCSELTSTQQDGSCSAN 444

[Close]

BLAST of MS016282 vs. TAIR 10
Match: AT4G23820.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 691.0 bits (1782), Expect = 6.1e-199
Identity = 323/437 (73.91%), Postives = 379/437 (86.73%), Query Frame = 0


Query: 5   FLFLFFFSAAAAAAADLTTCSGIVPMKYRNDKISIEDFGGVGDGRTLNTRAFREAIFRIQ 64
           FLF   F ++++      TCSGIVP++YR DKISI DFGGVGDGRT+NT+AFR AI+RIQ
Sbjct: 9   FLFSCLFVSSSSLGDSEATCSGIVPLRYRYDKISITDFGGVGDGRTVNTKAFRAAIYRIQ 68

Query: 65  HLKRRGGTLLYIPPGVFLTESFNLTSHMTLYLAKGAVIKAVQDSSDWPVIAPLPSYGRGR 124
           HLKRRGGTLLYIPPGV+LTESFNLTSHMTLYLAKGAVI+AVQD+ +WP+I PLPSYGRGR
Sbjct: 69  HLKRRGGTLLYIPPGVYLTESFNLTSHMTLYLAKGAVIRAVQDTWNWPLIDPLPSYGRGR 128

Query: 125 ERPGGRYVSFIHGDGVHDVVITGENGTIDGQGDAWWNMWKTGTLKFTRPNLVEFINSYNI 184
           E PGGRY+SFIHGDG+ DVVITG+NGTIDGQG+ WWNMW++ TLK+TRPNL+EF +S  I
Sbjct: 129 ELPGGRYMSFIHGDGLRDVVITGQNGTIDGQGEVWWNMWRSRTLKYTRPNLIEFKDSKEI 188

Query: 185 IISNVIFQNSPFWNIHPVYCRNVVVRYVTILAPRDSPNTDGIDPDSSNNVCIEDSYISTG 244
           IISNVIFQNSPFWNIHPVYC NVV+ +VTILAP+DSPNTDGIDPDSS NVCIEDSYISTG
Sbjct: 189 IISNVIFQNSPFWNIHPVYCSNVVIHHVTILAPQDSPNTDGIDPDSSYNVCIEDSYISTG 248

Query: 245 DDLVAVKSGWDEYGIAYGRSSYDITIRRVSGSSPFAGVAVGSETSGGVANVLAEHLSFFD 304
           DDLVA+KSGWD+YGIAYGR S +ITIRR++GSSPFAG+A+GSETSGG+ N++AEH++  +
Sbjct: 249 DDLVAIKSGWDQYGIAYGRPSSNITIRRITGSSPFAGIAIGSETSGGIKNIIAEHITLSN 308

Query: 305 MGVGINIKTNIGRGGFIKNITVSNVYMERSRKGLKIAGDTGDHPDDKFDPNALPIVKDIT 364
           MGVG+NIKTNIGRGG+IKNI +S+VY++ ++ G+KIAGDTGDHPD+ ++PNALP+VK I 
Sbjct: 309 MGVGVNIKTNIGRGGYIKNIKISDVYVDTAKYGIKIAGDTGDHPDENYNPNALPVVKGIH 368

Query: 365 IKNVWGVNVQQAGSIYGLKNSPFTGICLSNINLHGTTRPRSVPWTCSYVSGAALLVSPWP 424
           IKNVWGVNV+ AGSI GLK SPFTGICLS INLHG+       W CS VSG +L VSPWP
Sbjct: 369 IKNVWGVNVRNAGSIQGLKGSPFTGICLSEINLHGSLNSYKT-WKCSDVSGTSLKVSPWP 428

Query: 425 CSELTSTQQDGLCSDNF 442
           CSEL +T    LCS  F
Sbjct: 429 CSELRTTGGSNLCSSTF 444

[Close]

BLAST of MS016282 vs. TAIR 10
Match: AT5G41870.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 617.5 bits (1591), Expect = 8.5e-177
Identity = 291/419 (69.45%), Postives = 348/419 (83.05%), Query Frame = 0


Query: 23  TCSGIVPMKYRNDKISIEDFGGVGDGRTLNTRAFREAIFRIQHLKRRG-GTLLYIPPGVF 82
           TCSGIVPMK+RN+ +SI DFG VGDG+TLNT+AF  AI RI++      GTLLY+P GV+
Sbjct: 31  TCSGIVPMKHRNEMLSISDFGAVGDGKTLNTKAFNSAIDRIRNSNNSNEGTLLYVPRGVY 90

Query: 83  LTESFNLTSHMTLYLAKGAVIKAVQDSSDWPVIAPLPSYGRGRERPGGRYVSFIHGDGVH 142
           LT+SFNLTSHMTLYLA GAVIKAVQD+  WP+  PLPSYGRGRE PG RY+SFIHGDG++
Sbjct: 91  LTQSFNLTSHMTLYLADGAVIKAVQDTEKWPLTDPLPSYGRGREHPGRRYISFIHGDGLN 150

Query: 143 DVVITGENGTIDGQGDAWWNMWKTGTLKFTRPNLVEFINSYNIIISNVIFQNSPFWNIHP 202
           DVVITG NGTIDGQG+ WWNMW+ GTLKFTRP L+EF NS NI++S+V+ QNSPFW +HP
Sbjct: 151 DVVITGRNGTIDGQGEPWWNMWRHGTLKFTRPGLIEFNNSTNILVSHVVLQNSPFWTLHP 210

Query: 203 VYCRNVVVRYVTILAPRDSPNTDGIDPDSSNNVCIEDSYISTGDDLVAVKSGWDEYGIAY 262
           VYC NVVV +VTILAP DS NTDGIDPDSS+NVCIEDSYISTGDDLVAVKSGWDEYGIAY
Sbjct: 211 VYCSNVVVHHVTILAPTDSYNTDGIDPDSSSNVCIEDSYISTGDDLVAVKSGWDEYGIAY 270

Query: 263 GRSSYDITIRRVSGSSPFAGVAVGSETSGGVANVLAEHLSFFDMGVGINIKTNIGRGGFI 322
            R S DITIRR++GSSPFAG+A+GSETSGG+ NV  E+++ ++ G+GI+IKTNIGRGG I
Sbjct: 271 NRPSRDITIRRITGSSPFAGIAIGSETSGGIQNVTVENITLYNSGIGIHIKTNIGRGGSI 330

Query: 323 KNITVSNVYMERSRKGLKIAGDTGDHPDDKFDPNALPIVKDITIKNVWGVNVQQAGSIYG 382
           + IT+S VY+E+ R G+KI+GDTGDHPDDKF+ +ALPIV+ ITIKNVWG+ V++AG + G
Sbjct: 331 QGITISGVYLEKVRTGIKISGDTGDHPDDKFNTSALPIVRGITIKNVWGIKVERAGMVQG 390

Query: 383 LKNSPFTGICLSNINLHGTTRPRSVPWTCSYVSGAALLVSPWPCSELTSTQQDGLCSDN 441
           LK+SPFT +C SN+ L GT   RS  W CS V GAA  V+P PC EL++T Q G   +N
Sbjct: 391 LKDSPFTNLCFSNVTLTGT--KRSPIWKCSDVVGAADKVNPTPCPELSATTQQGGSCEN 447

[Close]

BLAST of MS016282 vs. TAIR 10
Match: AT4G33440.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 447.6 bits (1150), Expect = 1.2e-125
Identity = 219/412 (53.16%), Postives = 286/412 (69.42%), Query Frame = 0


Query: 20  DLTTCSGIVPMKYRNDKI--SIEDFGGVGDGRTLNTRAFREAIFRIQHLKRRGGTLLYIP 79
           D  TCSG        ++I  SI DFGGVGDG+T NT AFR A+  ++     GG  L +P
Sbjct: 53  DPVTCSGFFNHDPFPNRIVMSITDFGGVGDGKTSNTAAFRRAVRHLEGFAAEGGAQLNVP 112

Query: 80  PGVFLTESFNLTSHMTLYLAKGAVIKAVQDSSDWPVIAPLPSYGRGRERPGGRYVSFIHG 139
            G +L+ SFNLTS+ TL+L +GA+I   +D  +WP+I PLPSYGRGRERPGGR++S IHG
Sbjct: 113 EGTWLSGSFNLTSNFTLFLERGALILGSKDLDEWPIIEPLPSYGRGRERPGGRHISLIHG 172

Query: 140 DGVHDVVITGENGTIDGQGDAWWNMWKTGTLKFTRPNLVEFINSYNIIISNVIFQNSPFW 199
           D + +VVITGENGTIDGQG  WW +W   TL  TR +L+E  NS+NI+ISN+   NSPFW
Sbjct: 173 DNLTNVVITGENGTIDGQGKMWWELWWNRTLVHTRGHLIELKNSHNILISNLTLLNSPFW 232

Query: 200 NIHPVYCRNVVVRYVTILAPRDSPNTDGIDPDSSNNVCIEDSYISTGDDLVAVKSGWDEY 259
            IHPVYC NVV+R +TILAP ++PNTDGIDPDSS NVCIED YI +GDDLVAVKSGWD+Y
Sbjct: 233 TIHPVYCSNVVIRNMTILAPMNAPNTDGIDPDSSTNVCIEDCYIESGDDLVAVKSGWDQY 292

Query: 260 GIAYGRSSYDITIRRVSGSS-PFAGVAVGSETSGGVANVLAEHLSFFDMGVGINIKTNIG 319
           G+A  R S +I IRR+SG++   +GV +GSE SGG+ N+  E +  +D   G+ IKT+ G
Sbjct: 293 GMAVARPSSNIVIRRISGTTRTCSGVGIGSEMSGGIFNITVEDIHVWDSAAGLRIKTDKG 352

Query: 320 RGGFIKNITVSNVYMERSRKGLKIAGDTGDHPDDKFDPNALPIVKDITIKNVWGVNVQQA 379
           RGG+I NIT +NV +E+ +  ++ +  + DH DDK+DP ALP VK I I NV  +N ++A
Sbjct: 353 RGGYISNITFNNVLLEKVKVPIRFSSGSNDHSDDKWDPKALPRVKGIYISNVVSLNSRKA 412

Query: 380 GSIYGLKNSPFTGICLSNINLHGTTRPRSVPWTCSYVSGAALLVSPWPCSEL 429
             + G++ + F  +CL N+ L G   P++  W C  VSG A  V P  C +L
Sbjct: 413 PMLLGVEGTSFQDVCLRNVTLLGL--PKTEKWKCKDVSGYASDVFPLSCPQL 462

[Close]

BLAST of MS016282 vs. TAIR 10
Match: AT3G62110.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 442.2 bits (1136), Expect = 4.9e-124
Identity = 215/429 (50.12%), Postives = 289/429 (67.37%), Query Frame = 0


Query: 1   MLRFFLFLF------FFSAAAAAAADLTTCSGIVPMKYRNDKISIEDFGGVGDGRTLNTR 60
           M R FL L+      F+ A  +    L      +   +R   +SI +FG VGDG TLNT+
Sbjct: 1   MKRSFLLLYVLLVQAFYGAWCSVGESLHCEYSNLASLHRPHSVSITEFGAVGDGVTLNTK 60

Query: 61  AFREAIFRIQHLKRRGGTLLYIPPGVFLTESFNLTSHMTLYLAKGAVIKAVQDSSDWPVI 120
           AF+ A+F +     +GG  L++P G +LT SF+L SH+TL+L KGA I     S +WPV+
Sbjct: 61  AFQNALFYLNSFSDKGGAKLFVPAGQWLTGSFDLISHLTLWLDKGATILGSTSSENWPVV 120

Query: 121 APLPSYGRGRERPGGRYVSFIHGDGVHDVVITGENGTIDGQGDAWWNMWKTGTLKFTRPN 180
            PLPSYGRGRE PG R+ S I+G  + DVVITGENGTIDGQG  WW+ ++ G L +TRP+
Sbjct: 121 DPLPSYGRGRELPGRRHRSLIYGQNLTDVVITGENGTIDGQGTVWWDWFRNGELNYTRPH 180

Query: 181 LVEFINSYNIIISNVIFQNSPFWNIHPVYCRNVVVRYVTILAPRDSPNTDGIDPDSSNNV 240
           LVE +NS  +IISN+ F NSPFWNIHPVYCR+VVV+ +TILAP +SPNTDG+DPDSS NV
Sbjct: 181 LVELMNSTGLIISNLTFLNSPFWNIHPVYCRDVVVKNLTILAPLESPNTDGVDPDSSTNV 240

Query: 241 CIEDSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIRRVSG-SSPFAGVAVGSETSGGVA 300
           CIED YI TGDDLV++KSGWDEYGI+Y R S  I I R++G ++  +G+A+GSE SGGV+
Sbjct: 241 CIEDCYIVTGDDLVSIKSGWDEYGISYARPSSKIKINRLTGQTTSSSGIAIGSEMSGGVS 300

Query: 301 NVLAEHLSFFDMGVGINIKTNIGRGGFIKNITVSNVYMERSRKGLKIAGDTGDHPDDKFD 360
            +  + L  F+   GI IKT+ GRGG+++N+ + NV ++  +K ++  G  G+HPD+K+D
Sbjct: 301 EIYIKDLHLFNSNTGIRIKTSAGRGGYVRNVHILNVKLDNVKKAIRFTGKYGEHPDEKYD 360

Query: 361 PNALPIVKDITIKNVWGVNVQQAGSIYGLKNSPFTGICLSNINLHGTTRPRSVPWTCSYV 420
           P ALP ++ IT +NV G  +  AG + G++   F  IC  N+ L      +  PW CS V
Sbjct: 361 PKALPAIEKITFENVNGDGIGVAGLLEGIEGDVFKNICFLNVTLRVKKNSKKSPWECSNV 420

Query: 421 SGAALLVSP 423
            G +  VSP
Sbjct: 421 RGYSQWVSP 429

[Close]

BLAST of MS016282 vs. TAIR 10
Match: AT3G16850.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 412.5 bits (1059), Expect = 4.2e-115
Identity = 207/400 (51.75%), Postives = 277/400 (69.25%), Query Frame = 0


Query: 33  RNDKISIEDFGGVGDGRTLNTRAFREAIFRIQHLKRRGGTLLYIPPGVFLTESFNLTSHM 92
           R   +SI +FG VGDG+TLNT AF+ A+F ++    +GG  LY+PPG +LT SFNLTSH+
Sbjct: 36  RPHSVSILEFGAVGDGKTLNTIAFQNAVFYLKSFADKGGAQLYVPPGKWLTGSFNLTSHL 95

Query: 93  TLYLAKGAVIKAVQDSSDWPVIAPLPSYGRGRERPGGRYVSFIHGDGVHDVVITGENGTI 152
           TL+L KGA I A  D S W V++PLPSYGRG E PG RY S I+GD + DVVITGENGT 
Sbjct: 96  TLFLEKGATILASPDPSHWDVVSPLPSYGRGIELPGKRYRSLINGDNLIDVVITGENGTF 155

Query: 153 DGQGDAWWNMWKTGTLKFTRPNLVEFINSYNIIISNVIFQNSPFWNIHPVYCRNVVVRYV 212
           DGQG AWW   ++G+L ++RP+++EF++S +I+ISN+ F N+P  NIHPVYC  + +R V
Sbjct: 156 DGQGAAWWEWLESGSLNYSRPHIIEFVSSKHILISNLTFLNAPAINIHPVYCSQIHIRKV 215

Query: 213 TILAPRDSPNTDGIDPDSSNNVCIEDSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIRR 272
            I    DSP+  G+ PDSS+NVCIEDS I+ G D V++KSGWD+YGI YGR +  + IR 
Sbjct: 216 LIETSVDSPHVLGVAPDSSDNVCIEDSTINVGHDAVSLKSGWDQYGIHYGRPTTAVHIRN 275

Query: 273 VSGSSPF-AGVAVGSETSGGVANVLAEHLSFFDMGVGINIKTNIGRGGFIKNITVSNVYM 332
           +   SP  AG++ GSE SGGV++V  E L+     +G+  +T  GRGG+I+NIT+S+V +
Sbjct: 276 LRLKSPTGAGISFGSEMSGGVSDVTVERLNIHSSLIGVAFRTTRGRGGYIRNITISDVDL 335

Query: 333 ERSRKGLKIAGDTGDHPDDKFDPNALPIVKDITIKNVWGVNVQQAGSIYGLKNSPFTGIC 392
                 +   G TG HPDDKFD +ALP+V  I ++N  GV++  AG++ G+  SPFT IC
Sbjct: 336 TSVDTAIVANGHTGSHPDDKFDRDALPVVTHIVMRNFTGVDIGVAGNLTGIGESPFTSIC 395

Query: 393 LSNINLHGTTRPRSVPWTCSYVSGAALLVSPWPCSELTST 432
           L++I+L   TR     W CS VSG +  VSP PC EL S+
Sbjct: 396 LADIHLQ--TRSEE-SWICSNVSGFSDDVSPEPCQELMSS 432

The following BLAST results are available for this feature:

BLAST of MS016282 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022146051.1	1.5e-244	100.00	probable polygalacturonase [Momordica charantia]	[more]
XP_038876129.1	9.3e-242	91.46	probable polygalacturonase [Benincasa hispida]	[more]
XP_008437386.1	5.6e-239	90.34	PREDICTED: probable polygalacturonase [Cucumis melo] >KAA0042687.1 putative poly...	[more]
XP_004143871.1	2.1e-238	89.89	probable polygalacturonase [Cucumis sativus] >KGN50025.1 hypothetical protein Cs...	[more]
XP_022970040.1	6.9e-237	90.77	probable polygalacturonase [Cucurbita maxima] >XP_022970041.1 probable polygalac...	[more]

BLAST of MS016282 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A7PZL3	9.7e-117	49.88	Probable polygalacturonase OS=Vitis vinifera OX=29760 GN=GSVIVT00026920001 PE=1 ...	[more]
Q8RY29	4.0e-30	28.00	Polygalacturonase ADPG2 OS=Arabidopsis thaliana OX=3702 GN=ADPG2 PE=2 SV=2	[more]
O23147	2.8e-23	26.40	Polygalacturonase ADPG1 OS=Arabidopsis thaliana OX=3702 GN=ADPG1 PE=2 SV=1	[more]
Q02096	2.8e-23	26.47	Polygalacturonase OS=Persea americana OX=3435 PE=2 SV=1	[more]
Q7M1E7	1.3e-20	26.86	Polygalacturonase OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1	[more]

BLAST of MS016282 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CY77	7.4e-245	100.00	probable polygalacturonase OS=Momordica charantia OX=3673 GN=LOC111015353 PE=3 S...	[more]
A0A5A7TLS6	2.7e-239	90.34	Putative polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff...	[more]
A0A1S3ATJ9	2.7e-239	90.34	probable polygalacturonase OS=Cucumis melo OX=3656 GN=LOC103482817 PE=3 SV=1	[more]
A0A0A0KK36	1.0e-238	89.89	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G150430 PE=3 SV=1	[more]
A0A6J1I1Q1	3.3e-237	90.77	probable polygalacturonase OS=Cucurbita maxima OX=3661 GN=LOC111469049 PE=3 SV=1	[more]

BLAST of MS016282 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G23820.1	6.1e-199	73.91	Pectin lyase-like superfamily protein	[more]
AT5G41870.1	8.5e-177	69.45	Pectin lyase-like superfamily protein	[more]
AT4G33440.1	1.2e-125	53.16	Pectin lyase-like superfamily protein	[more]
AT3G62110.1	4.9e-124	50.12	Pectin lyase-like superfamily protein	[more]
AT3G16850.1	4.2e-115	51.75	Pectin lyase-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006626	Parallel beta-helix repeat	SMART	SM00710	pbh1	coord: 231..263 e-value: 990.0 score: 7.3 coord: 321..342 e-value: 620.0 score: 8.7 coord: 360..394 e-value: 7600.0 score: 0.9 coord: 265..286 e-value: 3200.0 score: 3.6 coord: 204..230 e-value: 180.0 score: 10.9 coord: 292..313 e-value: 2500.0 score: 4.4
IPR000743	Glycoside hydrolase, family 28	PFAM	PF00295	Glyco_hydro_28	coord: 133..414 e-value: 3.5E-40 score: 138.1
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 20..413 e-value: 1.6E-111 score: 375.1
None	No IPR available	PANTHER	PTHR31339	PECTIN LYASE-RELATED	coord: 9..438
None	No IPR available	PANTHER	PTHR31339:SF3	PECTIN LYASE-LIKE SUPERFAMILY PROTEIN	coord: 9..438
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 29..429

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS016282.1	MS016282.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
molecular_function	GO:0004650	polygalacturonase activity