MS016268 (gene) Bitter gourd (TR) v1

Overview


Name	MS016268
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	CUE domain-containing protein
Location	scaffold9_2: 1548048 .. 1550409 (-)
RNA-Seq Expression	MS016268
Synteny	MS016268

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCTGCTGTGGTGTGCGGAACCAAGAGATCCTTCTTTGAAGAACTCCCGCCTTCGCCTCCCATAGCAAAGCGGCTCCGCTGCTCCACTTCTACTTCGCCGATTAGGTTCGCCGCGCCGTCTCTTATTGATCAGCTTCACGATTTGTTTCCGCACATGGATCATCAGGTTTCATTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTTGTGGTTTTAGTTATAGTTTTGTTTGAATTGGAGAAACAAACACTCGTACTCCGTTTGAAGACGAGGATGCTGGTTGTTTTTGCTTTCCTTTTGTACGTTTAGTTCGTGCTTTTTTAGTCTAGCAGGAAGGATATGTAGTATCTAATATGATAATTCTTGTTAGGATTAGAACCTGATTCTCTATGGAATTTTCTGATATAATAGATCCTAGGGAGATAGAATTACTATATTCCTAATTGTTATATACGCCTCTGGATTGTGAATTATCAACTTATTTGACTTTGTTGCGGCATGAAATTGGAGTGGTCCTATTCGGTTTTAATGTGCAATGCATCTCTTTCGGTGTCTTGTTTGATAACTGCTGCATGTATGCTATAATTAACGTTGCAAGTTTGTTTCCCCCGGTTTTCTGGATCATGCTTTGATTTTGCCTTTCAGCTTCTTGCGAGAGCCCTTGAAGAATGTGGCAACGACTTGGATGCTGCCATCAAATGCTTGAGTGACCTCTGTTTGGGATCTGCTGCTGAGAATCCGGGTGCTACTGCTGAACCAGAGACAAATTTGGATCAAGGTATCATGAAATTCTAAATGATAAAGGAGATGCTTCATATGTATTTTCCTTGTTTCTGTTCGTTCACTCATGATCTTAGCTATATCATGATGACGAATTCATTAATGAATTCGTGGAACTAAATTCCTCCTTCAAGGAAAGGAAGGTTTAATTTTGTTGATGAGATTCGAGAAAATAGTTCTCAATTAGAATGTGATGCTTATTTCATGGAAGATATCCAATGGGTCCCCCTAAATTGCTGTCACTAGTTTGGATCACTAATCATACCCGTGCCTTGTCCATGTTAAAGAAGGTTTAAATTTTGATTAAAAAACGAGAATTAGAGTTCTCATTTGAAGTACTATGTATTCATTCGCTTAGAGTACAATGTATTATTTCTTCATGGAAGATACTCAATGGTTCCCTACCTACCTTGCTGTTTGTAGTTTGGTTTCATAAGCATTACAGAATTGGCTGGTACATTTACACAAACAGTTGGAAAGAAACTTTAAATTAAAATTGTTGGAGATTTAGTTCTGCAAGGGATTATAATCTTCTTTAATCTTCTGTTGTTGATGCTGTATTGCCCGACCATTTTGGAATATGTTTCTTTCAATCCGGCATCTATTTCTATGCCTTTCAGCATGCATTTAATGTAGAACTTTCATATGAATGTTGAACTCTGCATCATGCGATTTTCTGTGAAGGCCACTGTTCTCAACCTTTTGTTATCCTTGAACTTGCATTATTTTTTTCTTTTGTAAAGTTCAATAATTCATCATATGTTTAAAAAATTGACAGGTTCATTTGTCAACGATGGGGAGGCTGCTGCTTCTGAGAATTTGTCAGCACCAAGCAGTGTTTCTCTTGATGGTAGAGAATGGGTTGACTTGTTTGTAAGGGAAATGATGAGCGCTACCAGTGTAGATGATGCTAGGACCCGTGCAGCAAGAGCTTTAGAAGCTTTAGAAAACTCAATTAGTGCACGTGCTGGTGCTGATGCGGTACAAAATTTCCACAAGGTACTTCCATTTGAATGTTTTATGGTTCCAATAAATTTCCATGTCCTTGGTGGAAAATTTGGTATACTAGTAGGTTTTTGGAGTATTCGGGAGCAGTTAGAATTTGTGGCCTTACTCTCTTGTCAAAGATGTTGCTATAAAAATATCAAGGTAGGCATATAAACTTTTGACGTTTTCACTGTTATTCAGGAAAATATACAACTGAAGGAACAAATTGAAGTACTGCTACGAGAAAATACTATTCTTAAGCGAGCAGTTTCCATACAACATGAACGTCAGAAGGAGTTCGACGACAAGAACCTAGAGTTGCAGCACCTTAAACAGTTGGTATCTCAATATCAGGAGCAGCTGAGAACGCTAGAGGTCAGAATAAATTTTATAACTATTCTAATTATCTTCCCCAGTATTAATGGACCTGTTTGAAGTTCTTAGATACATCTTCATTTTGATATTACATTTCTCACCGGTGGAAAAAAAACTTGTCACTTTGTTACAGATAAACAACTATGCATTGACAATGCATTTGAAGCAGGCTCAGCAAAGCAGCTCGATTCCAGGGCGCTTCCATCCCGATGTGTTC

mRNA sequence

ATGTCTGCTGTGGTGTGCGGAACCAAGAGATCCTTCTTTGAAGAACTCCCGCCTTCGCCTCCCATAGCAAAGCGGCTCCGCTGCTCCACTTCTACTTCGCCGATTAGGTTCGCCGCGCCGTCTCTTATTGATCAGCTTCACGATTTGTTTCCGCACATGGATCATCAGCTTCTTGCGAGAGCCCTTGAAGAATGTGGCAACGACTTGGATGCTGCCATCAAATGCTTGAGTGACCTCTGTTTGGGATCTGCTGCTGAGAATCCGGGTGCTACTGCTGAACCAGAGACAAATTTGGATCAAGGTTCATTTGTCAACGATGGGGAGGCTGCTGCTTCTGAGAATTTGTCAGCACCAAGCAGTGTTTCTCTTGATGGTAGAGAATGGGTTGACTTGTTTGTAAGGGAAATGATGAGCGCTACCAGTGTAGATGATGCTAGGACCCGTGCAGCAAGAGCTTTAGAAGCTTTAGAAAACTCAATTAGTGCACGTGCTGGTGCTGATGCGGTACAAAATTTCCACAAGGAAAATATACAACTGAAGGAACAAATTGAAGTACTGCTACGAGAAAATACTATTCTTAAGCGAGCAGTTTCCATACAACATGAACGTCAGAAGGAGTTCGACGACAAGAACCTAGAGTTGCAGCACCTTAAACAGTTGGTATCTCAATATCAGGAGCAGCTGAGAACGCTAGAGATAAACAACTATGCATTGACAATGCATTTGAAGCAGGCTCAGCAAAGCAGCTCGATTCCAGGGCGCTTCCATCCCGATGTGTTC

Coding sequence (CDS)

ATGTCTGCTGTGGTGTGCGGAACCAAGAGATCCTTCTTTGAAGAACTCCCGCCTTCGCCTCCCATAGCAAAGCGGCTCCGCTGCTCCACTTCTACTTCGCCGATTAGGTTCGCCGCGCCGTCTCTTATTGATCAGCTTCACGATTTGTTTCCGCACATGGATCATCAGCTTCTTGCGAGAGCCCTTGAAGAATGTGGCAACGACTTGGATGCTGCCATCAAATGCTTGAGTGACCTCTGTTTGGGATCTGCTGCTGAGAATCCGGGTGCTACTGCTGAACCAGAGACAAATTTGGATCAAGGTTCATTTGTCAACGATGGGGAGGCTGCTGCTTCTGAGAATTTGTCAGCACCAAGCAGTGTTTCTCTTGATGGTAGAGAATGGGTTGACTTGTTTGTAAGGGAAATGATGAGCGCTACCAGTGTAGATGATGCTAGGACCCGTGCAGCAAGAGCTTTAGAAGCTTTAGAAAACTCAATTAGTGCACGTGCTGGTGCTGATGCGGTACAAAATTTCCACAAGGAAAATATACAACTGAAGGAACAAATTGAAGTACTGCTACGAGAAAATACTATTCTTAAGCGAGCAGTTTCCATACAACATGAACGTCAGAAGGAGTTCGACGACAAGAACCTAGAGTTGCAGCACCTTAAACAGTTGGTATCTCAATATCAGGAGCAGCTGAGAACGCTAGAGATAAACAACTATGCATTGACAATGCATTTGAAGCAGGCTCAGCAAAGCAGCTCGATTCCAGGGCGCTTCCATCCCGATGTGTTC

Protein sequence

MSAVVCGTKRSFFEELPPSPPIAKRLRCSTSTSPIRFAAPSLIDQLHDLFPHMDHQLLARALEECGNDLDAAIKCLSDLCLGSAAENPGATAEPETNLDQGSFVNDGEAAASENLSAPSSVSLDGREWVDLFVREMMSATSVDDARTRAARALEALENSISARAGADAVQNFHKENIQLKEQIEVLLRENTILKRAVSIQHERQKEFDDKNLELQHLKQLVSQYQEQLRTLEINNYALTMHLKQAQQSSSIPGRFHPDVF

Homology

[Close]

BLAST of MS016268 vs. NCBI nr
Match: XP_022153492.1 (uncharacterized protein LOC111020986 [Momordica charantia])

HSP 1 Score: 500.4 bits (1287), Expect = 9.6e-138
Identity = 259/260 (99.62%), Postives = 260/260 (100.00%), Query Frame = 0


Query: 1   MSAVVCGTKRSFFEELPPSPPIAKRLRCSTSTSPIRFAAPSLIDQLHDLFPHMDHQLLAR 60
           MSAVVCGTKRSFFEELPPSPPIAKRLRCSTSTSPIRFAAPSLIDQLHDLFPHMDHQLLAR
Sbjct: 1   MSAVVCGTKRSFFEELPPSPPIAKRLRCSTSTSPIRFAAPSLIDQLHDLFPHMDHQLLAR 60

Query: 61  ALEECGNDLDAAIKCLSDLCLGSAAENPGATAEPETNLDQGSFVNDGEAAASENLSAPSS 120
           ALEECGNDLDAAIKCLSDLCLGSAAENPGATAEPETNLDQGSFVNDGEAAASENLSAPSS
Sbjct: 61  ALEECGNDLDAAIKCLSDLCLGSAAENPGATAEPETNLDQGSFVNDGEAAASENLSAPSS 120

Query: 121 VSLDGREWVDLFVREMMSATSVDDARTRAARALEALENSISARAGADAVQNFHKENIQLK 180
           VSLDGREWVDLFVREMMSATSVDDARTRAARALEALENSISARAGADAVQNFHKENIQLK
Sbjct: 121 VSLDGREWVDLFVREMMSATSVDDARTRAARALEALENSISARAGADAVQNFHKENIQLK 180

Query: 181 EQIEVLLRENTILKRAVSIQHERQKEFDDKNLELQHLKQLVSQYQEQLRTLEINNYALTM 240
           EQIEVL+RENTILKRAVSIQHERQKEFDDKNLELQHLKQLVSQYQEQLRTLEINNYALTM
Sbjct: 181 EQIEVLIRENTILKRAVSIQHERQKEFDDKNLELQHLKQLVSQYQEQLRTLEINNYALTM 240

Query: 241 HLKQAQQSSSIPGRFHPDVF 261
           HLKQAQQSSSIPGRFHPDVF
Sbjct: 241 HLKQAQQSSSIPGRFHPDVF 260

[Close]

BLAST of MS016268 vs. NCBI nr
Match: XP_022949480.1 (uncharacterized protein LOC111452812 isoform X2 [Cucurbita moschata])

HSP 1 Score: 445.7 bits (1145), Expect = 2.8e-121
Identity = 230/260 (88.46%), Postives = 244/260 (93.85%), Query Frame = 0


Query: 1   MSAVVCGTKRSFFEELPPSPPIAKRLRCSTSTSPIRFAAPSLIDQLHDLFPHMDHQLLAR 60
           MSAVVCGTKRSFFEELPPSPP++KRLRCS++TSPIRFAA  LIDQL +LFP MDHQLL R
Sbjct: 1   MSAVVCGTKRSFFEELPPSPPVSKRLRCSSTTSPIRFAASCLIDQLQNLFPQMDHQLLVR 60

Query: 61  ALEECGNDLDAAIKCLSDLCLGSAAENPGATAEPETNLDQGSFVNDGEAAASENLSAPSS 120
            LEECGNDLDAAIK LS++CLGS  ENP AT   ETNLDQGSF ++GE AASENLSAPSS
Sbjct: 61  VLEECGNDLDAAIKSLSNMCLGSTVENPVATTAAETNLDQGSFADNGEPAASENLSAPSS 120

Query: 121 VSLDGREWVDLFVREMMSATSVDDARTRAARALEALENSISARAGADAVQNFHKENIQLK 180
           VSLDGREWVDLFVREMMSATSVDDARTRAARALEALE+SISARAGADA QNFHKEN+QLK
Sbjct: 121 VSLDGREWVDLFVREMMSATSVDDARTRAARALEALESSISARAGADAAQNFHKENMQLK 180

Query: 181 EQIEVLLRENTILKRAVSIQHERQKEFDDKNLELQHLKQLVSQYQEQLRTLEINNYALTM 240
           EQIEVLLRENTILKRAV+IQHERQKEF+DKNLELQHLKQLVSQYQEQLRTLE+NNYALTM
Sbjct: 181 EQIEVLLRENTILKRAVAIQHERQKEFEDKNLELQHLKQLVSQYQEQLRTLEVNNYALTM 240

Query: 241 HLKQAQQSSSIPGRFHPDVF 261
           HLKQAQQSSSIPGRFHPDVF
Sbjct: 241 HLKQAQQSSSIPGRFHPDVF 260

[Close]

BLAST of MS016268 vs. NCBI nr
Match: XP_023525880.1 (uncharacterized protein LOC111789361 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 441.4 bits (1134), Expect = 5.3e-120
Identity = 228/260 (87.69%), Postives = 243/260 (93.46%), Query Frame = 0


Query: 1   MSAVVCGTKRSFFEELPPSPPIAKRLRCSTSTSPIRFAAPSLIDQLHDLFPHMDHQLLAR 60
           MSAVVCGTKRSFFEELPPSPP++KRLRCS++TSPIRFAA  LIDQL +LFP MDHQLL R
Sbjct: 1   MSAVVCGTKRSFFEELPPSPPVSKRLRCSSTTSPIRFAASCLIDQLQNLFPQMDHQLLVR 60

Query: 61  ALEECGNDLDAAIKCLSDLCLGSAAENPGATAEPETNLDQGSFVNDGEAAASENLSAPSS 120
            LEECGNDLDAAIK LS++CLGS  ENP AT   ETNLDQGSF ++GE AASENLSAP+S
Sbjct: 61  VLEECGNDLDAAIKSLSNMCLGSTVENPVATTAAETNLDQGSFADNGEPAASENLSAPTS 120

Query: 121 VSLDGREWVDLFVREMMSATSVDDARTRAARALEALENSISARAGADAVQNFHKENIQLK 180
           VSLDGREWVDLFVREMMSATSVDDARTRAARALEALE+SISARAGADA QN HKEN+QLK
Sbjct: 121 VSLDGREWVDLFVREMMSATSVDDARTRAARALEALESSISARAGADAAQNSHKENMQLK 180

Query: 181 EQIEVLLRENTILKRAVSIQHERQKEFDDKNLELQHLKQLVSQYQEQLRTLEINNYALTM 240
           EQIEVLLRENTILKRAV+IQHERQKEF+DKNLELQHLKQLVSQYQEQLRTLE+NNYALTM
Sbjct: 181 EQIEVLLRENTILKRAVAIQHERQKEFEDKNLELQHLKQLVSQYQEQLRTLEVNNYALTM 240

Query: 241 HLKQAQQSSSIPGRFHPDVF 261
           HLKQAQQSSSIPGRFHPDVF
Sbjct: 241 HLKQAQQSSSIPGRFHPDVF 260

[Close]

BLAST of MS016268 vs. NCBI nr
Match: XP_022998357.1 (uncharacterized protein LOC111493023 [Cucurbita maxima])

HSP 1 Score: 441.4 bits (1134), Expect = 5.3e-120
Identity = 228/260 (87.69%), Postives = 242/260 (93.08%), Query Frame = 0


Query: 1   MSAVVCGTKRSFFEELPPSPPIAKRLRCSTSTSPIRFAAPSLIDQLHDLFPHMDHQLLAR 60
           MSAVVCGTKRSFFEELPPSPP++KRLRCS++TSPIRFAA  LIDQL +LFP MDHQLL R
Sbjct: 1   MSAVVCGTKRSFFEELPPSPPVSKRLRCSSTTSPIRFAASCLIDQLQNLFPQMDHQLLVR 60

Query: 61  ALEECGNDLDAAIKCLSDLCLGSAAENPGATAEPETNLDQGSFVNDGEAAASENLSAPSS 120
            LEECGNDLDAAIK LS++CLGS  ENP AT   ETNLDQGSF ++GE AASENLSAPS 
Sbjct: 61  VLEECGNDLDAAIKSLSNMCLGSTVENPVATTPAETNLDQGSFADNGEPAASENLSAPSR 120

Query: 121 VSLDGREWVDLFVREMMSATSVDDARTRAARALEALENSISARAGADAVQNFHKENIQLK 180
           VSLDGREWVDLFVREMMSATSV+DARTRAARALEALE+SISARAGADA QNFHKEN QLK
Sbjct: 121 VSLDGREWVDLFVREMMSATSVEDARTRAARALEALESSISARAGADAAQNFHKENTQLK 180

Query: 181 EQIEVLLRENTILKRAVSIQHERQKEFDDKNLELQHLKQLVSQYQEQLRTLEINNYALTM 240
           EQIEVLLRENTILKRAV+IQHERQKEF+DKNLELQHLKQLVSQYQEQLRTLE+NNYALTM
Sbjct: 181 EQIEVLLRENTILKRAVAIQHERQKEFEDKNLELQHLKQLVSQYQEQLRTLEVNNYALTM 240

Query: 241 HLKQAQQSSSIPGRFHPDVF 261
           HLKQAQQSSSIPGRFHPDVF
Sbjct: 241 HLKQAQQSSSIPGRFHPDVF 260

[Close]

BLAST of MS016268 vs. NCBI nr
Match: XP_022949479.1 (uncharacterized protein LOC111452812 isoform X1 [Cucurbita moschata])

HSP 1 Score: 439.1 bits (1128), Expect = 2.6e-119
Identity = 229/261 (87.74%), Postives = 243/261 (93.10%), Query Frame = 0


Query: 1   MSAVVCGTKRSFFEELPPSPPIAKRLRCSTSTSPIRFAAPSLIDQLHDLFPHMDHQLLAR 60
           MSAVVCGTKRSFFEELPPSPP++KRLRCS++TSPIRFAA  LIDQL +LFP MDHQLL R
Sbjct: 1   MSAVVCGTKRSFFEELPPSPPVSKRLRCSSTTSPIRFAASCLIDQLQNLFPQMDHQLLVR 60

Query: 61  ALEECGNDLDAAIKCLSDLCLGSAAENPGATAEPETNLDQ-GSFVNDGEAAASENLSAPS 120
            LEECGNDLDAAIK LS++CLGS  ENP AT   ETNLD  GSF ++GE AASENLSAPS
Sbjct: 61  VLEECGNDLDAAIKSLSNMCLGSTVENPVATTAAETNLDHGGSFADNGEPAASENLSAPS 120

Query: 121 SVSLDGREWVDLFVREMMSATSVDDARTRAARALEALENSISARAGADAVQNFHKENIQL 180
           SVSLDGREWVDLFVREMMSATSVDDARTRAARALEALE+SISARAGADA QNFHKEN+QL
Sbjct: 121 SVSLDGREWVDLFVREMMSATSVDDARTRAARALEALESSISARAGADAAQNFHKENMQL 180

Query: 181 KEQIEVLLRENTILKRAVSIQHERQKEFDDKNLELQHLKQLVSQYQEQLRTLEINNYALT 240
           KEQIEVLLRENTILKRAV+IQHERQKEF+DKNLELQHLKQLVSQYQEQLRTLE+NNYALT
Sbjct: 181 KEQIEVLLRENTILKRAVAIQHERQKEFEDKNLELQHLKQLVSQYQEQLRTLEVNNYALT 240

Query: 241 MHLKQAQQSSSIPGRFHPDVF 261
           MHLKQAQQSSSIPGRFHPDVF
Sbjct: 241 MHLKQAQQSSSIPGRFHPDVF 261

[Close]

BLAST of MS016268 vs. ExPASy TrEMBL
Match: A0A6J1DJ22 (uncharacterized protein LOC111020986 OS=Momordica charantia OX=3673 GN=LOC111020986 PE=4 SV=1)

HSP 1 Score: 500.4 bits (1287), Expect = 4.7e-138
Identity = 259/260 (99.62%), Postives = 260/260 (100.00%), Query Frame = 0


Query: 1   MSAVVCGTKRSFFEELPPSPPIAKRLRCSTSTSPIRFAAPSLIDQLHDLFPHMDHQLLAR 60
           MSAVVCGTKRSFFEELPPSPPIAKRLRCSTSTSPIRFAAPSLIDQLHDLFPHMDHQLLAR
Sbjct: 1   MSAVVCGTKRSFFEELPPSPPIAKRLRCSTSTSPIRFAAPSLIDQLHDLFPHMDHQLLAR 60

Query: 61  ALEECGNDLDAAIKCLSDLCLGSAAENPGATAEPETNLDQGSFVNDGEAAASENLSAPSS 120
           ALEECGNDLDAAIKCLSDLCLGSAAENPGATAEPETNLDQGSFVNDGEAAASENLSAPSS
Sbjct: 61  ALEECGNDLDAAIKCLSDLCLGSAAENPGATAEPETNLDQGSFVNDGEAAASENLSAPSS 120

Query: 121 VSLDGREWVDLFVREMMSATSVDDARTRAARALEALENSISARAGADAVQNFHKENIQLK 180
           VSLDGREWVDLFVREMMSATSVDDARTRAARALEALENSISARAGADAVQNFHKENIQLK
Sbjct: 121 VSLDGREWVDLFVREMMSATSVDDARTRAARALEALENSISARAGADAVQNFHKENIQLK 180

Query: 181 EQIEVLLRENTILKRAVSIQHERQKEFDDKNLELQHLKQLVSQYQEQLRTLEINNYALTM 240
           EQIEVL+RENTILKRAVSIQHERQKEFDDKNLELQHLKQLVSQYQEQLRTLEINNYALTM
Sbjct: 181 EQIEVLIRENTILKRAVSIQHERQKEFDDKNLELQHLKQLVSQYQEQLRTLEINNYALTM 240

Query: 241 HLKQAQQSSSIPGRFHPDVF 261
           HLKQAQQSSSIPGRFHPDVF
Sbjct: 241 HLKQAQQSSSIPGRFHPDVF 260

[Close]

BLAST of MS016268 vs. ExPASy TrEMBL
Match: A0A6J1GCW0 (uncharacterized protein LOC111452812 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111452812 PE=4 SV=1)

HSP 1 Score: 445.7 bits (1145), Expect = 1.4e-121
Identity = 230/260 (88.46%), Postives = 244/260 (93.85%), Query Frame = 0


Query: 1   MSAVVCGTKRSFFEELPPSPPIAKRLRCSTSTSPIRFAAPSLIDQLHDLFPHMDHQLLAR 60
           MSAVVCGTKRSFFEELPPSPP++KRLRCS++TSPIRFAA  LIDQL +LFP MDHQLL R
Sbjct: 1   MSAVVCGTKRSFFEELPPSPPVSKRLRCSSTTSPIRFAASCLIDQLQNLFPQMDHQLLVR 60

Query: 61  ALEECGNDLDAAIKCLSDLCLGSAAENPGATAEPETNLDQGSFVNDGEAAASENLSAPSS 120
            LEECGNDLDAAIK LS++CLGS  ENP AT   ETNLDQGSF ++GE AASENLSAPSS
Sbjct: 61  VLEECGNDLDAAIKSLSNMCLGSTVENPVATTAAETNLDQGSFADNGEPAASENLSAPSS 120

Query: 121 VSLDGREWVDLFVREMMSATSVDDARTRAARALEALENSISARAGADAVQNFHKENIQLK 180
           VSLDGREWVDLFVREMMSATSVDDARTRAARALEALE+SISARAGADA QNFHKEN+QLK
Sbjct: 121 VSLDGREWVDLFVREMMSATSVDDARTRAARALEALESSISARAGADAAQNFHKENMQLK 180

Query: 181 EQIEVLLRENTILKRAVSIQHERQKEFDDKNLELQHLKQLVSQYQEQLRTLEINNYALTM 240
           EQIEVLLRENTILKRAV+IQHERQKEF+DKNLELQHLKQLVSQYQEQLRTLE+NNYALTM
Sbjct: 181 EQIEVLLRENTILKRAVAIQHERQKEFEDKNLELQHLKQLVSQYQEQLRTLEVNNYALTM 240

Query: 241 HLKQAQQSSSIPGRFHPDVF 261
           HLKQAQQSSSIPGRFHPDVF
Sbjct: 241 HLKQAQQSSSIPGRFHPDVF 260

[Close]

BLAST of MS016268 vs. ExPASy TrEMBL
Match: A0A6J1K7Q5 (uncharacterized protein LOC111493023 OS=Cucurbita maxima OX=3661 GN=LOC111493023 PE=4 SV=1)

HSP 1 Score: 441.4 bits (1134), Expect = 2.6e-120
Identity = 228/260 (87.69%), Postives = 242/260 (93.08%), Query Frame = 0


Query: 1   MSAVVCGTKRSFFEELPPSPPIAKRLRCSTSTSPIRFAAPSLIDQLHDLFPHMDHQLLAR 60
           MSAVVCGTKRSFFEELPPSPP++KRLRCS++TSPIRFAA  LIDQL +LFP MDHQLL R
Sbjct: 1   MSAVVCGTKRSFFEELPPSPPVSKRLRCSSTTSPIRFAASCLIDQLQNLFPQMDHQLLVR 60

Query: 61  ALEECGNDLDAAIKCLSDLCLGSAAENPGATAEPETNLDQGSFVNDGEAAASENLSAPSS 120
            LEECGNDLDAAIK LS++CLGS  ENP AT   ETNLDQGSF ++GE AASENLSAPS 
Sbjct: 61  VLEECGNDLDAAIKSLSNMCLGSTVENPVATTPAETNLDQGSFADNGEPAASENLSAPSR 120

Query: 121 VSLDGREWVDLFVREMMSATSVDDARTRAARALEALENSISARAGADAVQNFHKENIQLK 180
           VSLDGREWVDLFVREMMSATSV+DARTRAARALEALE+SISARAGADA QNFHKEN QLK
Sbjct: 121 VSLDGREWVDLFVREMMSATSVEDARTRAARALEALESSISARAGADAAQNFHKENTQLK 180

Query: 181 EQIEVLLRENTILKRAVSIQHERQKEFDDKNLELQHLKQLVSQYQEQLRTLEINNYALTM 240
           EQIEVLLRENTILKRAV+IQHERQKEF+DKNLELQHLKQLVSQYQEQLRTLE+NNYALTM
Sbjct: 181 EQIEVLLRENTILKRAVAIQHERQKEFEDKNLELQHLKQLVSQYQEQLRTLEVNNYALTM 240

Query: 241 HLKQAQQSSSIPGRFHPDVF 261
           HLKQAQQSSSIPGRFHPDVF
Sbjct: 241 HLKQAQQSSSIPGRFHPDVF 260

[Close]

BLAST of MS016268 vs. ExPASy TrEMBL
Match: A0A6J1GCX8 (uncharacterized protein LOC111452812 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111452812 PE=4 SV=1)

HSP 1 Score: 439.1 bits (1128), Expect = 1.3e-119
Identity = 229/261 (87.74%), Postives = 243/261 (93.10%), Query Frame = 0


Query: 1   MSAVVCGTKRSFFEELPPSPPIAKRLRCSTSTSPIRFAAPSLIDQLHDLFPHMDHQLLAR 60
           MSAVVCGTKRSFFEELPPSPP++KRLRCS++TSPIRFAA  LIDQL +LFP MDHQLL R
Sbjct: 1   MSAVVCGTKRSFFEELPPSPPVSKRLRCSSTTSPIRFAASCLIDQLQNLFPQMDHQLLVR 60

Query: 61  ALEECGNDLDAAIKCLSDLCLGSAAENPGATAEPETNLDQ-GSFVNDGEAAASENLSAPS 120
            LEECGNDLDAAIK LS++CLGS  ENP AT   ETNLD  GSF ++GE AASENLSAPS
Sbjct: 61  VLEECGNDLDAAIKSLSNMCLGSTVENPVATTAAETNLDHGGSFADNGEPAASENLSAPS 120

Query: 121 SVSLDGREWVDLFVREMMSATSVDDARTRAARALEALENSISARAGADAVQNFHKENIQL 180
           SVSLDGREWVDLFVREMMSATSVDDARTRAARALEALE+SISARAGADA QNFHKEN+QL
Sbjct: 121 SVSLDGREWVDLFVREMMSATSVDDARTRAARALEALESSISARAGADAAQNFHKENMQL 180

Query: 181 KEQIEVLLRENTILKRAVSIQHERQKEFDDKNLELQHLKQLVSQYQEQLRTLEINNYALT 240
           KEQIEVLLRENTILKRAV+IQHERQKEF+DKNLELQHLKQLVSQYQEQLRTLE+NNYALT
Sbjct: 181 KEQIEVLLRENTILKRAVAIQHERQKEFEDKNLELQHLKQLVSQYQEQLRTLEVNNYALT 240

Query: 241 MHLKQAQQSSSIPGRFHPDVF 261
           MHLKQAQQSSSIPGRFHPDVF
Sbjct: 241 MHLKQAQQSSSIPGRFHPDVF 261

[Close]

BLAST of MS016268 vs. ExPASy TrEMBL
Match: A0A6J1GWH1 (uncharacterized protein LOC111457514 OS=Cucurbita moschata OX=3662 GN=LOC111457514 PE=4 SV=1)

HSP 1 Score: 434.1 bits (1115), Expect = 4.1e-118
Identity = 226/261 (86.59%), Postives = 238/261 (91.19%), Query Frame = 0


Query: 1   MSAVVCGTKRSFFEELPPSPPIAKRLRCSTSTSPIRFAAP-SLIDQLHDLFPHMDHQLLA 60
           MSAVVCGTKRSFFEE+PPSPPI+KRLRCS+STSPI F AP SLID LH LFPHMDHQLL 
Sbjct: 1   MSAVVCGTKRSFFEEIPPSPPISKRLRCSSSTSPITFPAPSSLIDHLHHLFPHMDHQLLV 60

Query: 61  RALEECGNDLDAAIKCLSDLCLGSAAENPGATAEPETNLDQGSFVNDGEAAASENLSAPS 120
           RALEECGNDLDAAIK LSDLCLGSA ENP ATAEPE NLDQGSF NDGEAAASEN S PS
Sbjct: 61  RALEECGNDLDAAIKSLSDLCLGSAVENPVATAEPEPNLDQGSFANDGEAAASENCSVPS 120

Query: 121 SVSLDGREWVDLFVREMMSATSVDDARTRAARALEALENSISARAGADAVQNFHKENIQL 180
           SVSLDGR+W+DLFV EM+SA  V DAR+RAARALEALENSISA AG DA QNFHKEN+QL
Sbjct: 121 SVSLDGRKWIDLFVVEMVSAIDVADARSRAARALEALENSISASAGVDAAQNFHKENMQL 180

Query: 181 KEQIEVLLRENTILKRAVSIQHERQKEFDDKNLELQHLKQLVSQYQEQLRTLEINNYALT 240
           KEQIEVLLRENTILKRAV+IQHERQKEF+ KN+ELQHLKQLV QYQEQLRTLEINNYALT
Sbjct: 181 KEQIEVLLRENTILKRAVAIQHERQKEFEGKNIELQHLKQLVCQYQEQLRTLEINNYALT 240

Query: 241 MHLKQAQQSSSIPGRFHPDVF 261
           MHLKQ +QSSSIPGRFHPDVF
Sbjct: 241 MHLKQTEQSSSIPGRFHPDVF 261

[Close]

BLAST of MS016268 vs. TAIR 10
Match: AT5G32440.1 (Ubiquitin system component Cue protein )

HSP 1 Score: 276.2 bits (705), Expect = 2.7e-74
Identity = 151/268 (56.34%), Postives = 204/268 (76.12%), Query Frame = 0


Query: 1   MSAVVCGTKRSFFEEL-PPSPPIAKRLRCSTSTSPIRFAAP------SLIDQLHDLFPHM 60
           MSA+VCG KRS FE+L   SPP++K+LRC +S+S  RF+ P       L+D L  +FP M
Sbjct: 1   MSAIVCG-KRSLFEDLAAASPPVSKKLRCFSSSSSSRFSPPIPPSSSLLLDHLAAIFPDM 60

Query: 61  DHQLLARALEECGNDLDAAIKCLSDLCLGSAAEN-PGATAEPETNLDQGSFVNDGEAAAS 120
           D Q+L RA+EECG+DLD+AI+CL+ L L SA +N   AT +    + + +     + +A 
Sbjct: 61  DKQILERAIEECGDDLDSAIRCLNQLRLESANKNSDSATNQSPVVIQEPNVEPQQQGSAK 120

Query: 121 ENLSAPSSVSLDGREWVDLFVREMMSATSVDDARTRAARALEALENSISARAGADAVQNF 180
           E    P+ ++LDG EWV+LFVREMM+A+ + DA+ RAARALEALE SI+AR G DA+QN 
Sbjct: 121 EE---PNVLNLDGTEWVELFVREMMNASDMKDAKARAARALEALEKSINARTGTDAMQNL 180

Query: 181 HKENIQLKEQIEVLLRENTILKRAVSIQHERQKEFDDKNLELQHLKQLVSQYQEQLRTLE 240
            +EN+ LK+Q+E +++EN++LKRAV  Q +RQ+E +D++ ELQHL+QLV+QYQEQLRTLE
Sbjct: 181 QQENMMLKQQLEAIVQENSLLKRAVVTQQKRQRESEDQSQELQHLRQLVTQYQEQLRTLE 240

Query: 241 INNYALTMHLKQAQQSSSIPGRFHPDVF 261
           +NNYALT+HLKQAQQ+SSIPGR+HPDVF
Sbjct: 241 VNNYALTLHLKQAQQNSSIPGRYHPDVF 264

[Close]

BLAST of MS016268 vs. TAIR 10
Match: AT5G32440.3 (Ubiquitin system component Cue protein )

HSP 1 Score: 275.0 bits (702), Expect = 6.1e-74
Identity = 150/267 (56.18%), Postives = 200/267 (74.91%), Query Frame = 0


Query: 1   MSAVVCGTKRSFFEEL-PPSPPIAKRLRCSTSTSPIRFAAP------SLIDQLHDLFPHM 60
           MSA+VCG KRS FE+L   SPP++K+LRC +S+S  RF+ P       L+D L  +FP M
Sbjct: 1   MSAIVCG-KRSLFEDLAAASPPVSKKLRCFSSSSSSRFSPPIPPSSSLLLDHLAAIFPDM 60

Query: 61  DHQLLARALEECGNDLDAAIKCLSDLCLGSAAENPGATAEPETNLDQGSFVNDGEAAASE 120
           D Q+L RA+EECG+DLD+AI+CL+ L L SA +N  +       + Q   V   +   S 
Sbjct: 61  DKQILERAIEECGDDLDSAIRCLNQLRLESANKNSDSATNQSPVVIQEPNVEPQQQGRSA 120

Query: 121 NLSAPSSVSLDGREWVDLFVREMMSATSVDDARTRAARALEALENSISARAGADAVQNFH 180
               P+ ++LDG EWV+LFVREMM+A+ + DA+ RAARALEALE SI+AR G DA+QN  
Sbjct: 121 K-EEPNVLNLDGTEWVELFVREMMNASDMKDAKARAARALEALEKSINARTGTDAMQNLQ 180

Query: 181 KENIQLKEQIEVLLRENTILKRAVSIQHERQKEFDDKNLELQHLKQLVSQYQEQLRTLEI 240
           +EN+ LK+Q+E +++EN++LKRAV  Q +RQ+E +D++ ELQHL+QLV+QYQEQLRTLE+
Sbjct: 181 QENMMLKQQLEAIVQENSLLKRAVVTQQKRQRESEDQSQELQHLRQLVTQYQEQLRTLEV 240

Query: 241 NNYALTMHLKQAQQSSSIPGRFHPDVF 261
           NNYALT+HLKQAQQ+SSIPGR+HPDVF
Sbjct: 241 NNYALTLHLKQAQQNSSIPGRYHPDVF 265

[Close]

BLAST of MS016268 vs. TAIR 10
Match: AT1G80040.1 (FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: chloroplast; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Ubiquitin system component Cue (InterPro:IPR003892); BEST Arabidopsis thaliana protein match is: Ubiquitin system component Cue protein (TAIR:AT5G32440.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 209.1 bits (531), Expect = 4.1e-54
Identity = 125/262 (47.71%), Postives = 174/262 (66.41%), Query Frame = 0


Query: 1   MSAVVCGTKRSFFEELPPSPPIAKRLRC-STSTSPIRFAAP-SLIDQLHDLFPHMDHQLL 60
           MSAV CGTKRS+F++   SPP +KR RC S S SPI  + P S +DQLH  FPH++  +L
Sbjct: 1   MSAVYCGTKRSYFDD-NSSPPSSKRFRCFSPSNSPIWSSPPSSSLDQLHSAFPHIELTVL 60

Query: 61  ARALEECGNDLDAAIKCLSDLCLGSAAENPGATAEPETNLDQGSFVNDGEAAASENLSAP 120
            +ALE+ G+D +AA+K L      S+ E      + E     G+   + +A    N   P
Sbjct: 61  VKALEDNGSDFNAAMKSL--YSFASSEEK-----KAEELAAGGAATQETDAVCGGN--PP 120

Query: 121 SSVSLDGREWVDLFVREMMSATSVDDARTRAARALEALENSISARAGADAVQNFHKENIQ 180
           +S    G +WV+L VRE++ ++  DDA+ RAAR LEALE  +SARA  +A   F +E + 
Sbjct: 121 TS----GDDWVELLVREVLQSSGTDDAKVRAARVLEALEKMLSARAREEAGNKFQEEKVA 180

Query: 181 LKEQIEVLLRENTILKRAVSIQHERQKEFDDKNLELQHLKQLVSQYQEQLRTLEINNYAL 240
           +++Q+E L+++NT+LKRAV+IQHERQK  +D N +L  LKQLV QYQE+LR LE+NNYAL
Sbjct: 181 VQQQVETLVKDNTVLKRAVAIQHERQKALEDANHQLGLLKQLVPQYQEKLRNLEVNNYAL 240

Query: 241 TMHLKQAQQSSSIPGRFHPDVF 261
            M L+Q +  +S+P RF+PDVF
Sbjct: 241 RMQLQQVEHGNSMPARFNPDVF 248

[Close]

BLAST of MS016268 vs. TAIR 10
Match: AT1G80040.3 (FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: chloroplast; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 15 growth stages; BEST Arabidopsis thaliana protein match is: Ubiquitin system component Cue protein (TAIR:AT5G32440.1). )

HSP 1 Score: 203.4 bits (516), Expect = 2.3e-52
Identity = 126/273 (46.15%), Postives = 175/273 (64.10%), Query Frame = 0


Query: 1   MSAVVCGTKRSFFEELPPSPPIAKRLRC-STSTSPIRFAAP-SLIDQLHDLFPHMD---- 60
           MSAV CGTKRS+F++   SPP +KR RC S S SPI  + P S +DQLH  FPH++    
Sbjct: 1   MSAVYCGTKRSYFDD-NSSPPSSKRFRCFSPSNSPIWSSPPSSSLDQLHSAFPHIELTVA 60

Query: 61  -------HQLLARALEECGNDLDAAIKCLSDLCLGSAAENPGATAEPETNLDQGSFVNDG 120
                   Q+L +ALE+ G+D +AA+K L      S+ E      + E     G+   + 
Sbjct: 61  SKIHVSVAQVLVKALEDNGSDFNAAMKSL--YSFASSEEK-----KAEELAAGGAATQET 120

Query: 121 EAAASENLSAPSSVSLDGREWVDLFVREMMSATSVDDARTRAARALEALENSISARAGAD 180
           +A    N   P+S    G +WV+L VRE++ ++  DDA+ RAAR LEALE  +SARA  +
Sbjct: 121 DAVCGGN--PPTS----GDDWVELLVREVLQSSGTDDAKVRAARVLEALEKMLSARAREE 180

Query: 181 AVQNFHKENIQLKEQIEVLLRENTILKRAVSIQHERQKEFDDKNLELQHLKQLVSQYQEQ 240
           A   F +E + +++Q+E L+++NT+LKRAV+IQHERQK  +D N +L  LKQLV QYQE+
Sbjct: 181 AGNKFQEEKVAVQQQVETLVKDNTVLKRAVAIQHERQKALEDANHQLGLLKQLVPQYQEK 240

Query: 241 LRTLEINNYALTMHLKQAQQSSSIPGRFHPDVF 261
           LR LE+NNYAL M L+Q +  +S+P RF+PDVF
Sbjct: 241 LRNLEVNNYALRMQLQQVEHGNSMPARFNPDVF 259

[Close]

BLAST of MS016268 vs. TAIR 10
Match: AT5G32440.2 (Ubiquitin system component Cue protein )

HSP 1 Score: 154.8 bits (390), Expect = 9.2e-38
Identity = 92/188 (48.94%), Postives = 128/188 (68.09%), Query Frame = 0


Query: 1   MSAVVCGTKRSFFEEL-PPSPPIAKRLRCSTSTSPIRFAAP------SLIDQLHDLFPHM 60
           MSA+VCG KRS FE+L   SPP++K+LRC +S+S  RF+ P       L+D L  +FP M
Sbjct: 1   MSAIVCG-KRSLFEDLAAASPPVSKKLRCFSSSSSSRFSPPIPPSSSLLLDHLAAIFPDM 60

Query: 61  DHQLLARALEECGNDLDAAIKCLSDLCLGSAAEN-PGATAEPETNLDQGSFVNDGEAAAS 120
           D Q+L RA+EECG+DLD+AI+CL+ L L SA +N   AT +    + + +     + +A 
Sbjct: 61  DKQILERAIEECGDDLDSAIRCLNQLRLESANKNSDSATNQSPVVIQEPNVEPQQQGSAK 120

Query: 121 ENLSAPSSVSLDGREWVDLFVREMMSATSVDDARTRAARALEALENSISARAGADAVQNF 180
           E    P+ ++LDG EWV+LFVREMM+A+ + DA+ RAARALEALE SI+AR G DA+QN 
Sbjct: 121 EE---PNVLNLDGTEWVELFVREMMNASDMKDAKARAARALEALEKSINARTGTDAMQNL 180

The following BLAST results are available for this feature:

BLAST of MS016268 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022153492.1	9.6e-138	99.62	uncharacterized protein LOC111020986 [Momordica charantia]	[more]
XP_022949480.1	2.8e-121	88.46	uncharacterized protein LOC111452812 isoform X2 [Cucurbita moschata]	[more]
XP_023525880.1	5.3e-120	87.69	uncharacterized protein LOC111789361 [Cucurbita pepo subsp. pepo]	[more]
XP_022998357.1	5.3e-120	87.69	uncharacterized protein LOC111493023 [Cucurbita maxima]	[more]
XP_022949479.1	2.6e-119	87.74	uncharacterized protein LOC111452812 isoform X1 [Cucurbita moschata]	[more]

BLAST of MS016268 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of MS016268 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DJ22	4.7e-138	99.62	uncharacterized protein LOC111020986 OS=Momordica charantia OX=3673 GN=LOC111020...	[more]
A0A6J1GCW0	1.4e-121	88.46	uncharacterized protein LOC111452812 isoform X2 OS=Cucurbita moschata OX=3662 GN...	[more]
A0A6J1K7Q5	2.6e-120	87.69	uncharacterized protein LOC111493023 OS=Cucurbita maxima OX=3661 GN=LOC111493023...	[more]
A0A6J1GCX8	1.3e-119	87.74	uncharacterized protein LOC111452812 isoform X1 OS=Cucurbita moschata OX=3662 GN...	[more]
A0A6J1GWH1	4.1e-118	86.59	uncharacterized protein LOC111457514 OS=Cucurbita moschata OX=3662 GN=LOC1114575...	[more]

BLAST of MS016268 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G32440.1	2.7e-74	56.34	Ubiquitin system component Cue protein	[more]
AT5G32440.3	6.1e-74	56.18	Ubiquitin system component Cue protein	[more]
AT1G80040.1	4.1e-54	47.71	FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknow...	[more]
AT1G80040.3	2.3e-52	46.15	FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknow...	[more]
AT5G32440.2	9.2e-38	48.94	Ubiquitin system component Cue protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 207..234
None	No IPR available	COILS	Coil	Coil	coord: 169..196
None	No IPR available	GENE3D	1.10.8.10		coord: 39..81 e-value: 1.7E-6 score: 29.5
None	No IPR available	PANTHER	PTHR31245	UBIQUITIN SYSTEM COMPONENT CUE PROTEIN	coord: 1..260
None	No IPR available	PANTHER	PTHR31245:SF20	F18B13.13 PROTEIN	coord: 1..260
None	No IPR available	CDD	cd14279	CUE	coord: 41..76 e-value: 9.72503E-10 score: 50.9297
IPR003892	Ubiquitin system component CUE	PFAM	PF02845	CUE	coord: 41..76 e-value: 6.8E-8 score: 32.1
IPR003892	Ubiquitin system component CUE	PROSITE	PS51140	CUE	coord: 38..84 score: 11.624031
IPR009060	UBA-like superfamily	SUPERFAMILY	46934	UBA-like	coord: 41..82

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS016268.1	MS016268.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005515	protein binding
molecular_function	GO:0043130	ubiquitin binding