MS016248 (gene) Bitter gourd (TR) v1

Overview


Name	MS016248
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	3-isopropylmalate dehydrogenase
Location	scaffold9_2: 1413576 .. 1418050 (-)
RNA-Seq Expression	MS016248
Synteny	MS016248

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

GCGGCGGCGGCTCTGCATCTCAACGCAAAACCGGTAAATCTTTTTTCCCGATCCATCACCGTTTCGAAACCATCGCCGAAGCTATTCCGGGTCAGCTGCTCCGCCGGCACTTCTCCTTCCAGGCGCTATTCCATCGCTTTGCTTCCCGGCGATGGCGTTGGTCCGGAAGTCATTGCCGTCGCGAAGGATGTTCTGAACTTCGCTGCTACGCTTGAAGGTCCCTCCTCTTAATCAGTCTCTGTGTCTCCCCACCCCCTATTCTTCTTTTACTTTTCTGTTACATGGTAGTTGTGTTTGTGTTTTGTTGATGGGGTACTGGATTGTTTGATTAGTAAGTTCAAAATTGCATTGGAATTCTGTTGCACTCAGCTTAAAGACGGTAACGCAGTTCAATTGTTTGCTGAGAAATAAAATTATGAAATTGCGATGAGCTGAAGAAAGTTGGAAGAATTTATTTACGTTCGTGCATATTGTTTTAAAATTATAGGGTGAGATTTTACATTTCGTTTGGGTCTTTTGACGACCATTGGCTTCTGTTACTTTAGGGATTGGGTTTGATTACAAAGAGATGCCTGTCGGTGGAGCTGCACTTGATTTGACTGGGGTTCCATTGCCAGACGAAACGCTTTTAGCAGCCAAGCAATCTGATGCCGTTCTATTTGGCGCAATTGGAGGGTATTTATATACGTTGTGCTATTTACATTGATTTCTTTCATAATTTTTTAAAAACTGGTATGGTTGGTTGAATCATCTCATGACTCATTCCTTTTATCAAGATACAAATGGGATGATAATCCAAAACATTTGAAGCCAGAGACTGGGTTGCTTCAACTTCGAAAAGGTCTTGAAGTATTCGCTAATTTGCGGCCTGCAACTGTCTTTCCCCAGGTAAACTACTACATTATCCTCCCTCCCCTACACCAATATAAACTATTTACAAATGAAAATACAAGGCCCAAGGTCCTATTTTCGTTTCTTTTGTTTTTTTGGCCTGCGATCATTTTTATTGCAATATAAAAGTGCATGGCATAGGTGTTTCAATAATCTGAAGACGGGAAATTATCTTGGTTTGAAGCCCTATTAGATACCCTCGTTTCATCTAAATGTTAAACTTAGCCTCATGCCAAGAAGGATAGATTCAGACGTATGAATTAATAATGTCATGACTTAATATGTTGATATATTTCTTCAGTGGTCAACTTGCGAGAATTATTTTTCAATGGTTTGCATAAATGGCATTATATTCAGAAGCGTTGGTGTTGAACTTCATTTCTTATTAAAGAGCATCCAAGTTGGCCCACCTCTGGAAAAACTATACCAGTGTTGCTCCTAAATGAAGCATGGGAATATTACTTGTATTTATTTTAATTGAGATGCATAAATTCATGTGCAGTGGTGTGAGTTCATGAGGAAGCACCTCTGGGTATAAGGTTAAAGTCCTATATGGGGTACTCTGTTACCTATTTAATTATTCCATTTCTTACCCCTTCAGTTAGTGGACGCTTCAACTTTGAAGAAGGAGGTTGCTGAAGGTGTTGATCTCATGGTCATAAGGGAGCTCACAGGAGGTAAATTTTTTCCCCTCCCGCTTCAAACGAGATTCCATGTGTTGATTTCCCTCCATATCTAAATTTTGGGTTTTGAATAAAGGTGCCATGATATCTTTCACCTGGATTAGGTATTTATTTTGGGCAACCAAGAGGTTTTAAAACCAGTGAAAATGGGGAAGAAATTGGATTTAATACTGAAGTGTATGCAACTCATGAGGTAACTTTTTCTTTTTAATTCAACATCCCTGGGAGCTTGCATATAAAGTCTTCAACTCCTTTTTATTATACGACGTGCTCTTATCTTTAAATAAATTGTTTTATGGAAAGCAGCTCAATATTAGTTGGATTAAAAATATTTCTGATAAGCATTCCTTTCAGTCATCCTTGCTTACTCGGAACAATTTCATACTAATCTATGAATCCATTCTTCAAAGCGATCGTTGATACATTCATGAAGAAGTTATTCCTTTTTTTTTCCCTTTATTTCTCTCTCTCACTCCTTTTTTTTTTAAACCATAAAAAGCATAACTGTTTTATATAAATTTTAAATTGGAAGTGCAGTTGTTCCATATTTCATTCAGTTGTTCCATATTTCATTTTTCTTGAAATTAAGATTACATGCTTAATTTGTTTTTATATCTAAAATAATCCTCTTCTTTATTTTATAACTATCTATTACGTCTATTTGAACAATTACGTTTCAGTTTGTTAATGTCTTCTGCTTCAGATCGACCGCGTTGCTCGAGTGGCATTTGAGAATGCTCGGAAGCGACAAGGAAGAGTTTGCTCTGTAGATAAAGCAAATGTGTTGGAGGTGCTTTTAAGAATTTGATTGTTATTTTTGTTGATTGATTGGTATTGTGGTCACTGGTCATTCTAATTCATAAGAACAACCTTTTCATCCTTTTGGTGCAATCTCTATGATAGTTTGTTTCATCTAATTTTCATTGAGCCACAACCCACGAAATATAGCATTTAGGCACAAAAACCTTTTTGTCTAATACAAAAGTTAAAAGGTAAAACTGCTTTCTCTCCTCTTGGGAAAGTTGAAACGATACAAGAAGATATGCTTATCACTTTCTCAAGAGCTTTGGGGACCTGGAATCAGGAACATCATAAATTTTGCACTTCTTAGCCAGATGGCTTCACCATCCATTCATAAATAAAGCATTCCTGACGATGGATGGTGGGAGCTTTCTAAGGAATCTAGTATTGGAGGAAGTTCGGGATAGTATCATGTTTCTTTCTTCTGCTTGGTCTTCTCTGTCATTTTCTAAAAAAAATTTCCCTCTCTGTAATTATTGCAATTTTTCTATCATAGCCAATTTCTGTCTTTCTGTAACCCTTAAGTTAGGGCTTACCCTTTTATGTATATTTCATTTTTTTCCCATTGAAATAGTTCTGTTTCTGATTGGAAAAGGTTATTAGGAAATTTGAGATAATAACCCCAGACCATTCCTTTATTTTGCTGGAAATAGGTTCAGATTTGAATTTTGAGAATGTGTAACTGTCTCATCTGCTTTCCTCCAAGTTTATTTATTTGAAGATTAATGATCTACGTCAAACATTCAGTTCAAGGTTTCCCTACTGTATTGACATATAACCATGCGTTTCAATAGAGCTTAATATGTTATGTACGATCTATGAATGTAAGAACTTACCACAGTTAAAACTTGAAAGTAGTGGGGTCACATGTTCCTTTATGTAGAAACCTTACAATGGAACTAACTTTTTTGCAGGCTTCAATGCTTTGGAGGAAAAGAGTGATAGCAATAGCTGCAGAATATCCTGATATTGAACTCTCGCACATGTACGTTGATAATGCTGCAATGCAGCTAATTCGCAATCCAAAGCAGGTCTGCATTGAAAAATAATCTTATTTTCCAGAAGGGGCAAGAAAGTGAACTTTCATAGCCCCTATATGATAGGAAAGATAAGTTCTATTTGGACTGATTGTGAAAATTTGCAGTTCGACACAATTTTGACAAACAACATATTCGGTGATATTTTGTCGGATGCTGCTTCCATGATTACTGGAAGTATTGGGATGCTTCCATCTGCAAGTCTTCGTGAATCGGTAGTCATATCATTCTCTCGCATTGTTATCCTCTCTAAATTTTCTTCATTTTCCTTCTATAACAAAATGAATTGTTTGCACAGGGACCTGGACTATTTGAACCTATTCATGGCTCAGCTCCTGATATTGCTGGAAAGGTTTGTGTTCTTAAAATATCAGCATATTATAAGTAGGATGTAAAGTTTTATGCCATAAGATAACATGGTTTTATATGTAGGATAAGGCAAATCCATTAGCAACCGTTCTAAGTGCTGCTATGCTTCTAAAGTATGGCCTTGGAGAAGAAAATGCTGCCAAAAGAGTTGAGGTTGCAGTTTTGGATACTCTAGACAGAGGATTTAGGACTGGTGACATATACTCGGCAGGATGTGTAAGTATACAGTGGAGGGAGAAATTTTCTCCCCCTCTCTCTCAATTTTGTCTTTGAATATGGATAAATTTAAACATCTGAATGAGAAAAAGAAAAGAAAAAAAGTAAAAACCTGACCGAAATGTCCGATAGCAAACAAATCGACCTCAACAAATTTACTTTTACAATCATGAAAATAGATCATCTAGCTAGCTTTTGGGTGTGTTTGAGAGTGATTTTGGCATGACGTATCTGCTACTCTATAGGTAAAATGGTTGTAGTTCACTTGAATTTTTTTTGGGTGGATTTCCACGGTGTATCATGTATGTTAATGCCCAGTTGCTCTCTTTTACTTTTGATAACTATTATTTAATTCCTCCTCTTGGTCATTAGTCCAATGATTTTTTCTTAACACTCGACTGCACAGAAACTTGTTGGATGCAAAGAGATGGGTGATGAGCTATTAAAATCAATAGAGTCCTATGAACCTGCTACAGTT

mRNA sequence

GCGGCGGCGGCTCTGCATCTCAACGCAAAACCGGTAAATCTTTTTTCCCGATCCATCACCGTTTCGAAACCATCGCCGAAGCTATTCCGGGTCAGCTGCTCCGCCGGCACTTCTCCTTCCAGGCGCTATTCCATCGCTTTGCTTCCCGGCGATGGCGTTGGTCCGGAAGTCATTGCCGTCGCGAAGGATGTTCTGAACTTCGCTGCTACGCTTGAAGGGATTGGGTTTGATTACAAAGAGATGCCTGTCGGTGGAGCTGCACTTGATTTGACTGGGGTTCCATTGCCAGACGAAACGCTTTTAGCAGCCAAGCAATCTGATGCCGTTCTATTTGGCGCAATTGGAGGATACAAATGGGATGATAATCCAAAACATTTGAAGCCAGAGACTGGGTTGCTTCAACTTCGAAAAGGTCTTGAAGTATTCGCTAATTTGCGGCCTGCAACTGTCTTTCCCCAGTTAGTGGACGCTTCAACTTTGAAGAAGGAGGTTGCTGAAGGTGTTGATCTCATGGTCATAAGGGAGCTCACAGGAGGTATTTATTTTGGGCAACCAAGAGGTTTTAAAACCAGTGAAAATGGGGAAGAAATTGGATTTAATACTGAAGTGTATGCAACTCATGAGATCGACCGCGTTGCTCGAGTGGCATTTGAGAATGCTCGGAAGCGACAAGGAAGAGTTTGCTCTGTAGATAAAGCAAATGTGTTGGAGGCTTCAATGCTTTGGAGGAAAAGAGTGATAGCAATAGCTGCAGAATATCCTGATATTGAACTCTCGCACATGTACGTTGATAATGCTGCAATGCAGCTAATTCGCAATCCAAAGCAGTTCGACACAATTTTGACAAACAACATATTCGGTGATATTTTGTCGGATGCTGCTTCCATGATTACTGGAAGTATTGGGATGCTTCCATCTGCAAGTCTTCGTGAATCGGGACCTGGACTATTTGAACCTATTCATGGCTCAGCTCCTGATATTGCTGGAAAGGATAAGGCAAATCCATTAGCAACCGTTCTAAGTGCTGCTATGCTTCTAAAGTATGGCCTTGGAGAAGAAAATGCTGCCAAAAGAGTTGAGGTTGCAGTTTTGGATACTCTAGACAGAGGATTTAGGACTGGTGACATATACTCGGCAGGATGTAAACTTGTTGGATGCAAAGAGATGGGTGATGAGCTATTAAAATCAATAGAGTCCTATGAACCTGCTACAGTT

Coding sequence (CDS)

GCGGCGGCGGCTCTGCATCTCAACGCAAAACCGGTAAATCTTTTTTCCCGATCCATCACCGTTTCGAAACCATCGCCGAAGCTATTCCGGGTCAGCTGCTCCGCCGGCACTTCTCCTTCCAGGCGCTATTCCATCGCTTTGCTTCCCGGCGATGGCGTTGGTCCGGAAGTCATTGCCGTCGCGAAGGATGTTCTGAACTTCGCTGCTACGCTTGAAGGGATTGGGTTTGATTACAAAGAGATGCCTGTCGGTGGAGCTGCACTTGATTTGACTGGGGTTCCATTGCCAGACGAAACGCTTTTAGCAGCCAAGCAATCTGATGCCGTTCTATTTGGCGCAATTGGAGGATACAAATGGGATGATAATCCAAAACATTTGAAGCCAGAGACTGGGTTGCTTCAACTTCGAAAAGGTCTTGAAGTATTCGCTAATTTGCGGCCTGCAACTGTCTTTCCCCAGTTAGTGGACGCTTCAACTTTGAAGAAGGAGGTTGCTGAAGGTGTTGATCTCATGGTCATAAGGGAGCTCACAGGAGGTATTTATTTTGGGCAACCAAGAGGTTTTAAAACCAGTGAAAATGGGGAAGAAATTGGATTTAATACTGAAGTGTATGCAACTCATGAGATCGACCGCGTTGCTCGAGTGGCATTTGAGAATGCTCGGAAGCGACAAGGAAGAGTTTGCTCTGTAGATAAAGCAAATGTGTTGGAGGCTTCAATGCTTTGGAGGAAAAGAGTGATAGCAATAGCTGCAGAATATCCTGATATTGAACTCTCGCACATGTACGTTGATAATGCTGCAATGCAGCTAATTCGCAATCCAAAGCAGTTCGACACAATTTTGACAAACAACATATTCGGTGATATTTTGTCGGATGCTGCTTCCATGATTACTGGAAGTATTGGGATGCTTCCATCTGCAAGTCTTCGTGAATCGGGACCTGGACTATTTGAACCTATTCATGGCTCAGCTCCTGATATTGCTGGAAAGGATAAGGCAAATCCATTAGCAACCGTTCTAAGTGCTGCTATGCTTCTAAAGTATGGCCTTGGAGAAGAAAATGCTGCCAAAAGAGTTGAGGTTGCAGTTTTGGATACTCTAGACAGAGGATTTAGGACTGGTGACATATACTCGGCAGGATGTAAACTTGTTGGATGCAAAGAGATGGGTGATGAGCTATTAAAATCAATAGAGTCCTATGAACCTGCTACAGTT

Protein sequence

AAAALHLNAKPVNLFSRSITVSKPSPKLFRVSCSAGTSPSRRYSIALLPGDGVGPEVIAVAKDVLNFAATLEGIGFDYKEMPVGGAALDLTGVPLPDETLLAAKQSDAVLFGAIGGYKWDDNPKHLKPETGLLQLRKGLEVFANLRPATVFPQLVDASTLKKEVAEGVDLMVIRELTGGIYFGQPRGFKTSENGEEIGFNTEVYATHEIDRVARVAFENARKRQGRVCSVDKANVLEASMLWRKRVIAIAAEYPDIELSHMYVDNAAMQLIRNPKQFDTILTNNIFGDILSDAASMITGSIGMLPSASLRESGPGLFEPIHGSAPDIAGKDKANPLATVLSAAMLLKYGLGEENAAKRVEVAVLDTLDRGFRTGDIYSAGCKLVGCKEMGDELLKSIESYEPATV

Homology

[Close]

BLAST of MS016248 vs. NCBI nr
Match: XP_022153420.1 (3-isopropylmalate dehydrogenase 2, chloroplastic-like [Momordica charantia])

HSP 1 Score: 798.9 bits (2062), Expect = 2.0e-227
Identity = 405/405 (100.00%), Postives = 405/405 (100.00%), Query Frame = 0


Query: 1   AAAALHLNAKPVNLFSRSITVSKPSPKLFRVSCSAGTSPSRRYSIALLPGDGVGPEVIAV 60
           AAAALHLNAKPVNLFSRSITVSKPSPKLFRVSCSAGTSPSRRYSIALLPGDGVGPEVIAV
Sbjct: 2   AAAALHLNAKPVNLFSRSITVSKPSPKLFRVSCSAGTSPSRRYSIALLPGDGVGPEVIAV 61

Query: 61  AKDVLNFAATLEGIGFDYKEMPVGGAALDLTGVPLPDETLLAAKQSDAVLFGAIGGYKWD 120
           AKDVLNFAATLEGIGFDYKEMPVGGAALDLTGVPLPDETLLAAKQSDAVLFGAIGGYKWD
Sbjct: 62  AKDVLNFAATLEGIGFDYKEMPVGGAALDLTGVPLPDETLLAAKQSDAVLFGAIGGYKWD 121

Query: 121 DNPKHLKPETGLLQLRKGLEVFANLRPATVFPQLVDASTLKKEVAEGVDLMVIRELTGGI 180
           DNPKHLKPETGLLQLRKGLEVFANLRPATVFPQLVDASTLKKEVAEGVDLMVIRELTGGI
Sbjct: 122 DNPKHLKPETGLLQLRKGLEVFANLRPATVFPQLVDASTLKKEVAEGVDLMVIRELTGGI 181

Query: 181 YFGQPRGFKTSENGEEIGFNTEVYATHEIDRVARVAFENARKRQGRVCSVDKANVLEASM 240
           YFGQPRGFKTSENGEEIGFNTEVYATHEIDRVARVAFENARKRQGRVCSVDKANVLEASM
Sbjct: 182 YFGQPRGFKTSENGEEIGFNTEVYATHEIDRVARVAFENARKRQGRVCSVDKANVLEASM 241

Query: 241 LWRKRVIAIAAEYPDIELSHMYVDNAAMQLIRNPKQFDTILTNNIFGDILSDAASMITGS 300
           LWRKRVIAIAAEYPDIELSHMYVDNAAMQLIRNPKQFDTILTNNIFGDILSDAASMITGS
Sbjct: 242 LWRKRVIAIAAEYPDIELSHMYVDNAAMQLIRNPKQFDTILTNNIFGDILSDAASMITGS 301

Query: 301 IGMLPSASLRESGPGLFEPIHGSAPDIAGKDKANPLATVLSAAMLLKYGLGEENAAKRVE 360
           IGMLPSASLRESGPGLFEPIHGSAPDIAGKDKANPLATVLSAAMLLKYGLGEENAAKRVE
Sbjct: 302 IGMLPSASLRESGPGLFEPIHGSAPDIAGKDKANPLATVLSAAMLLKYGLGEENAAKRVE 361

Query: 361 VAVLDTLDRGFRTGDIYSAGCKLVGCKEMGDELLKSIESYEPATV 406
           VAVLDTLDRGFRTGDIYSAGCKLVGCKEMGDELLKSIESYEPATV
Sbjct: 362 VAVLDTLDRGFRTGDIYSAGCKLVGCKEMGDELLKSIESYEPATV 406

[Close]

BLAST of MS016248 vs. NCBI nr
Match: XP_023525901.1 (3-isopropylmalate dehydrogenase, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 750.4 bits (1936), Expect = 8.3e-213
Identity = 376/405 (92.84%), Postives = 394/405 (97.28%), Query Frame = 0


Query: 1   AAAALHLNAKPVNLFSRSITVSKPSPKLFRVSCSAGTSPSRRYSIALLPGDGVGPEVIAV 60
           AAA LHLNAKP N FS SI++SK SPKLFRV+CSAGTSPSRR++IALLPGDGVGPEVIAV
Sbjct: 8   AAATLHLNAKPSNFFSPSISLSKTSPKLFRVTCSAGTSPSRRFNIALLPGDGVGPEVIAV 67

Query: 61  AKDVLNFAATLEGIGFDYKEMPVGGAALDLTGVPLPDETLLAAKQSDAVLFGAIGGYKWD 120
           AKDVLN AA+LEGIGFDYKEMPVGGAALDLTGVPLPDETL+AAKQSDAVLFGAIGGYKWD
Sbjct: 68  AKDVLNLAASLEGIGFDYKEMPVGGAALDLTGVPLPDETLIAAKQSDAVLFGAIGGYKWD 127

Query: 121 DNPKHLKPETGLLQLRKGLEVFANLRPATVFPQLVDASTLKKEVAEGVDLMVIRELTGGI 180
           DNPKHLKPETGLLQLRKGLEVFANLRPATVFPQLV+ASTLK+EVAEGVDLMV+RELTGGI
Sbjct: 128 DNPKHLKPETGLLQLRKGLEVFANLRPATVFPQLVNASTLKREVAEGVDLMVVRELTGGI 187

Query: 181 YFGQPRGFKTSENGEEIGFNTEVYATHEIDRVARVAFENARKRQGRVCSVDKANVLEASM 240
           YFGQPRGFKTSENGEEIGFNTEVYA HEIDRVARVAFENARKRQGRVCSVDKANVLEASM
Sbjct: 188 YFGQPRGFKTSENGEEIGFNTEVYAAHEIDRVARVAFENARKRQGRVCSVDKANVLEASM 247

Query: 241 LWRKRVIAIAAEYPDIELSHMYVDNAAMQLIRNPKQFDTILTNNIFGDILSDAASMITGS 300
           LWRKRV AIAAEYPDIELSHMYVDNAAMQLIRNPKQFDTILTNNIFGDILSDAASMITGS
Sbjct: 248 LWRKRVTAIAAEYPDIELSHMYVDNAAMQLIRNPKQFDTILTNNIFGDILSDAASMITGS 307

Query: 301 IGMLPSASLRESGPGLFEPIHGSAPDIAGKDKANPLATVLSAAMLLKYGLGEENAAKRVE 360
           IGMLPSASLRESGPGLFEPIHGSAPDIAG+DKANPLATVLSAAMLLKYG+GEE+AAKR+E
Sbjct: 308 IGMLPSASLRESGPGLFEPIHGSAPDIAGQDKANPLATVLSAAMLLKYGIGEESAAKRIE 367

Query: 361 VAVLDTLDRGFRTGDIYSAGCKLVGCKEMGDELLKSIESYEPATV 406
            AVLDTL+RGFRTGDIYSAGCKLVGCK MG+ELLKSIES++ ATV
Sbjct: 368 AAVLDTLERGFRTGDIYSAGCKLVGCKAMGEELLKSIESHKLATV 412

[Close]

BLAST of MS016248 vs. NCBI nr
Match: KAG6606821.1 (hypothetical protein SDJN03_00163, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 750.0 bits (1935), Expect = 1.1e-212
Identity = 375/405 (92.59%), Postives = 394/405 (97.28%), Query Frame = 0


Query: 1   AAAALHLNAKPVNLFSRSITVSKPSPKLFRVSCSAGTSPSRRYSIALLPGDGVGPEVIAV 60
           AAA LHLNAKP N FS SI++SK SPKLFRV+CSAGTSPSRR++IALLPGDGVGPEVIAV
Sbjct: 8   AAATLHLNAKPSNFFSPSISLSKTSPKLFRVTCSAGTSPSRRFNIALLPGDGVGPEVIAV 67

Query: 61  AKDVLNFAATLEGIGFDYKEMPVGGAALDLTGVPLPDETLLAAKQSDAVLFGAIGGYKWD 120
           AKDVLN AA+LEGIGFDYKEMPVGGAALDLTGVPLPDETL+AAKQSDAVLFGAIGGYKWD
Sbjct: 68  AKDVLNLAASLEGIGFDYKEMPVGGAALDLTGVPLPDETLIAAKQSDAVLFGAIGGYKWD 127

Query: 121 DNPKHLKPETGLLQLRKGLEVFANLRPATVFPQLVDASTLKKEVAEGVDLMVIRELTGGI 180
           DNPKHLKPETGLLQLRKGLEVFANLRPATVFPQLV+ASTLK+EVAEGVDLMV+RELTGGI
Sbjct: 128 DNPKHLKPETGLLQLRKGLEVFANLRPATVFPQLVNASTLKREVAEGVDLMVVRELTGGI 187

Query: 181 YFGQPRGFKTSENGEEIGFNTEVYATHEIDRVARVAFENARKRQGRVCSVDKANVLEASM 240
           YFGQPRGFKTSENGEEIGFNTEVYA HEIDRVARVAFENARKRQGRVCSVDKANVLEASM
Sbjct: 188 YFGQPRGFKTSENGEEIGFNTEVYAAHEIDRVARVAFENARKRQGRVCSVDKANVLEASM 247

Query: 241 LWRKRVIAIAAEYPDIELSHMYVDNAAMQLIRNPKQFDTILTNNIFGDILSDAASMITGS 300
           LWRKRV AIAAEYPD+ELSHMYVDNAAMQLIRNPKQFDTILTNNIFGDILSDAASMITGS
Sbjct: 248 LWRKRVTAIAAEYPDVELSHMYVDNAAMQLIRNPKQFDTILTNNIFGDILSDAASMITGS 307

Query: 301 IGMLPSASLRESGPGLFEPIHGSAPDIAGKDKANPLATVLSAAMLLKYGLGEENAAKRVE 360
           IGMLPSASLRESGPGLFEPIHGSAPDIAG+DKANPLATVLSAAMLLKYG+GEE+AAKR+E
Sbjct: 308 IGMLPSASLRESGPGLFEPIHGSAPDIAGQDKANPLATVLSAAMLLKYGIGEESAAKRIE 367

Query: 361 VAVLDTLDRGFRTGDIYSAGCKLVGCKEMGDELLKSIESYEPATV 406
            AVLDTL+RGFRTGDIYSAGCKLVGCK MG+ELLKSIES++ ATV
Sbjct: 368 AAVLDTLERGFRTGDIYSAGCKLVGCKAMGEELLKSIESHKLATV 412

[Close]

BLAST of MS016248 vs. NCBI nr
Match: KAG7036527.1 (hypothetical protein SDJN02_00145, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 750.0 bits (1935), Expect = 1.1e-212
Identity = 375/405 (92.59%), Postives = 394/405 (97.28%), Query Frame = 0


Query: 1   AAAALHLNAKPVNLFSRSITVSKPSPKLFRVSCSAGTSPSRRYSIALLPGDGVGPEVIAV 60
           AAA LHLNAKP N FS SI++SK SPKLFRV+CSAGTSPSRR++IALLPGDGVGPEVIAV
Sbjct: 39  AAATLHLNAKPSNFFSPSISLSKTSPKLFRVTCSAGTSPSRRFNIALLPGDGVGPEVIAV 98

Query: 61  AKDVLNFAATLEGIGFDYKEMPVGGAALDLTGVPLPDETLLAAKQSDAVLFGAIGGYKWD 120
           AKDVLN AA+LEGIGFDYKEMPVGGAALDLTGVPLPDETL+AAKQSDAVLFGAIGGYKWD
Sbjct: 99  AKDVLNLAASLEGIGFDYKEMPVGGAALDLTGVPLPDETLIAAKQSDAVLFGAIGGYKWD 158

Query: 121 DNPKHLKPETGLLQLRKGLEVFANLRPATVFPQLVDASTLKKEVAEGVDLMVIRELTGGI 180
           DNPKHLKPETGLLQLRKGLEVFANLRPATVFPQLV+ASTLK+EVAEGVDLMV+RELTGGI
Sbjct: 159 DNPKHLKPETGLLQLRKGLEVFANLRPATVFPQLVNASTLKREVAEGVDLMVVRELTGGI 218

Query: 181 YFGQPRGFKTSENGEEIGFNTEVYATHEIDRVARVAFENARKRQGRVCSVDKANVLEASM 240
           YFGQPRGFKTSENGEEIGFNTEVYA HEIDRVARVAFENARKRQGRVCSVDKANVLEASM
Sbjct: 219 YFGQPRGFKTSENGEEIGFNTEVYAAHEIDRVARVAFENARKRQGRVCSVDKANVLEASM 278

Query: 241 LWRKRVIAIAAEYPDIELSHMYVDNAAMQLIRNPKQFDTILTNNIFGDILSDAASMITGS 300
           LWRKRV AIAAEYPD+ELSHMYVDNAAMQLIRNPKQFDTILTNNIFGDILSDAASMITGS
Sbjct: 279 LWRKRVTAIAAEYPDVELSHMYVDNAAMQLIRNPKQFDTILTNNIFGDILSDAASMITGS 338

Query: 301 IGMLPSASLRESGPGLFEPIHGSAPDIAGKDKANPLATVLSAAMLLKYGLGEENAAKRVE 360
           IGMLPSASLRESGPGLFEPIHGSAPDIAG+DKANPLATVLSAAMLLKYG+GEE+AAKR+E
Sbjct: 339 IGMLPSASLRESGPGLFEPIHGSAPDIAGQDKANPLATVLSAAMLLKYGIGEESAAKRIE 398

Query: 361 VAVLDTLDRGFRTGDIYSAGCKLVGCKEMGDELLKSIESYEPATV 406
            AVLDTL+RGFRTGDIYSAGCKLVGCK MG+ELLKSIES++ ATV
Sbjct: 399 AAVLDTLERGFRTGDIYSAGCKLVGCKAMGEELLKSIESHKLATV 443

[Close]

BLAST of MS016248 vs. NCBI nr
Match: XP_022948488.1 (3-isopropylmalate dehydrogenase, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 748.8 bits (1932), Expect = 2.4e-212
Identity = 374/405 (92.35%), Postives = 394/405 (97.28%), Query Frame = 0


Query: 1   AAAALHLNAKPVNLFSRSITVSKPSPKLFRVSCSAGTSPSRRYSIALLPGDGVGPEVIAV 60
           AAA LHLNAKP N FS SI++SK SPKLFRV+CSAGTSPSRR++IALLPGDGVGPEVIAV
Sbjct: 3   AAATLHLNAKPSNFFSPSISLSKTSPKLFRVTCSAGTSPSRRFNIALLPGDGVGPEVIAV 62

Query: 61  AKDVLNFAATLEGIGFDYKEMPVGGAALDLTGVPLPDETLLAAKQSDAVLFGAIGGYKWD 120
           AKDVLN AA+LEGIGFDYKEMPVGGAALDLTGVPLPDETL+AAKQSDAVLFGAIGGYKWD
Sbjct: 63  AKDVLNLAASLEGIGFDYKEMPVGGAALDLTGVPLPDETLIAAKQSDAVLFGAIGGYKWD 122

Query: 121 DNPKHLKPETGLLQLRKGLEVFANLRPATVFPQLVDASTLKKEVAEGVDLMVIRELTGGI 180
           DNPKHLKPETGLLQLRKGLEVFANLRPATVFPQLV+ASTLK+EVAEGVDLMV+RELTGGI
Sbjct: 123 DNPKHLKPETGLLQLRKGLEVFANLRPATVFPQLVNASTLKREVAEGVDLMVVRELTGGI 182

Query: 181 YFGQPRGFKTSENGEEIGFNTEVYATHEIDRVARVAFENARKRQGRVCSVDKANVLEASM 240
           YFGQPRGFKT+ENGEEIGFNTEVYA HEIDRVARVAFENARKRQGRVCSVDKANVLEASM
Sbjct: 183 YFGQPRGFKTTENGEEIGFNTEVYAAHEIDRVARVAFENARKRQGRVCSVDKANVLEASM 242

Query: 241 LWRKRVIAIAAEYPDIELSHMYVDNAAMQLIRNPKQFDTILTNNIFGDILSDAASMITGS 300
           LWRKRV AIAAEYPD+ELSHMYVDNAAMQLIRNPKQFDTILTNNIFGDILSDAASMITGS
Sbjct: 243 LWRKRVTAIAAEYPDVELSHMYVDNAAMQLIRNPKQFDTILTNNIFGDILSDAASMITGS 302

Query: 301 IGMLPSASLRESGPGLFEPIHGSAPDIAGKDKANPLATVLSAAMLLKYGLGEENAAKRVE 360
           IGMLPSASLRESGPGLFEPIHGSAPDIAG+DKANPLATVLSAAMLLKYG+GEE+AAKR+E
Sbjct: 303 IGMLPSASLRESGPGLFEPIHGSAPDIAGQDKANPLATVLSAAMLLKYGIGEESAAKRIE 362

Query: 361 VAVLDTLDRGFRTGDIYSAGCKLVGCKEMGDELLKSIESYEPATV 406
            AVLDTL+RGFRTGDIYSAGCKLVGCK MG+ELLKSIES++ ATV
Sbjct: 363 AAVLDTLERGFRTGDIYSAGCKLVGCKAMGEELLKSIESHKLATV 407

[Close]

BLAST of MS016248 vs. ExPASy Swiss-Prot
Match: P29102 (3-isopropylmalate dehydrogenase, chloroplastic OS=Brassica napus OX=3708 PE=2 SV=1)

HSP 1 Score: 628.6 bits (1620), Expect = 4.8e-179
Identity = 311/406 (76.60%), Postives = 361/406 (88.92%), Query Frame = 0


Query: 2   AAALHLNAKPVNLFS--RSITVSKPSPKLFRVSCSAGTSPSRRYSIALLPGDGVGPEVIA 61
           AAAL  N +PV   +  R++T  + SP  FRV C+A +   +RY+I LLPGDG+GPEVI+
Sbjct: 2   AAALQTNIRPVKFPATLRALT-KQSSPAPFRVRCAAASPGKKRYNITLLPGDGIGPEVIS 61

Query: 62  VAKDVLNFAATLEGIGFDYKEMPVGGAALDLTGVPLPDETLLAAKQSDAVLFGAIGGYKW 121
           +AK+VL  A +LEG+ F ++EMPVGGAALDL GVPLP+ET+ AAK+SDAVL GAIGGYKW
Sbjct: 62  IAKNVLQQAGSLEGLEFSFQEMPVGGAALDLVGVPLPEETVSAAKESDAVLLGAIGGYKW 121

Query: 122 DDNPKHLKPETGLLQLRKGLEVFANLRPATVFPQLVDASTLKKEVAEGVDLMVIRELTGG 181
           D N KHLKPETGLLQLR GL+VFANLRPATV PQLVDASTLK+EVAEGVDLMV+RELTGG
Sbjct: 122 DKNEKHLKPETGLLQLRAGLKVFANLRPATVLPQLVDASTLKREVAEGVDLMVVRELTGG 181

Query: 182 IYFGQPRGFKTSENGEEIGFNTEVYATHEIDRVARVAFENARKRQGRVCSVDKANVLEAS 241
           IYFG PRG KT+ENGEE+G+NTEVYA HEIDR+ARVAFE ARKR+G++CSVDKANVL+AS
Sbjct: 182 IYFGVPRGIKTNENGEEVGYNTEVYAAHEIDRIARVAFETARKRRGKLCSVDKANVLDAS 241

Query: 242 MLWRKRVIAIAAEYPDIELSHMYVDNAAMQLIRNPKQFDTILTNNIFGDILSDAASMITG 301
           +LWR+RV A+AAEYPD+ELSHMYVDNAAMQL+R+PKQFDTI+TNNIFGDILSD ASMITG
Sbjct: 242 ILWRRRVTALAAEYPDVELSHMYVDNAAMQLVRDPKQFDTIVTNNIFGDILSDEASMITG 301

Query: 302 SIGMLPSASLRESGPGLFEPIHGSAPDIAGKDKANPLATVLSAAMLLKYGLGEENAAKRV 361
           SIGMLPSASL +SGPGLFEPIHGSAPDIAG+DKANPLAT+LSAAMLLKYGLGEE AAKR+
Sbjct: 302 SIGMLPSASLSDSGPGLFEPIHGSAPDIAGQDKANPLATILSAAMLLKYGLGEEKAAKRI 361

Query: 362 EVAVLDTLDRGFRTGDIYSAGCKLVGCKEMGDELLKSIESYEPATV 406
           E AVL  L++GFRTGDIYSAG KLVGCKEMG+E+LKS++S+  A+V
Sbjct: 362 EDAVLGALNKGFRTGDIYSAGTKLVGCKEMGEEVLKSVDSHVQASV 406

[Close]

BLAST of MS016248 vs. ExPASy Swiss-Prot
Match: P93832 (3-isopropylmalate dehydrogenase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IMDH2 PE=1 SV=1)

HSP 1 Score: 626.7 bits (1615), Expect = 1.8e-178
Identity = 306/404 (75.74%), Postives = 357/404 (88.37%), Query Frame = 0


Query: 2   AAALHLNAKPVNLFSRSITVSKPSPKLFRVSCSAGTSPSRRYSIALLPGDGVGPEVIAVA 61
           AAAL  N + V + +    VSK S   FRV C+  +   +RY+I LLPGDG+GPEV+++A
Sbjct: 2   AAALQTNIRTVKVPATFRAVSKQSLAPFRVRCAVASPGKKRYTITLLPGDGIGPEVVSIA 61

Query: 62  KDVLNFAATLEGIGFDYKEMPVGGAALDLTGVPLPDETLLAAKQSDAVLFGAIGGYKWDD 121
           K+VL  A +LEG+ F+++EMP+GGAALDL GVPLP+ET+ AAK+SDAVL GAIGGYKWD+
Sbjct: 62  KNVLQQAGSLEGVEFNFREMPIGGAALDLVGVPLPEETISAAKESDAVLLGAIGGYKWDN 121

Query: 122 NPKHLKPETGLLQLRKGLEVFANLRPATVFPQLVDASTLKKEVAEGVDLMVIRELTGGIY 181
           N KHL+PE GLLQ+R  L+VFANLRPATV PQLVDASTLK+EVAEGVDLMV+RELTGGIY
Sbjct: 122 NEKHLRPEKGLLQIRAALKVFANLRPATVLPQLVDASTLKREVAEGVDLMVVRELTGGIY 181

Query: 182 FGQPRGFKTSENGEEIGFNTEVYATHEIDRVARVAFENARKRQGRVCSVDKANVLEASML 241
           FG+PRG KT+ENGEE+GFNTEVYA HEIDR+ARVAFE ARKR+G++CSVDKANVLEAS+L
Sbjct: 182 FGEPRGIKTNENGEEVGFNTEVYAAHEIDRIARVAFETARKRRGKLCSVDKANVLEASIL 241

Query: 242 WRKRVIAIAAEYPDIELSHMYVDNAAMQLIRNPKQFDTILTNNIFGDILSDAASMITGSI 301
           WRKRV A+A+EYPD+ELSHMYVDNAAMQL+R+PKQFDTI+TNNIFGDILSD ASMITGSI
Sbjct: 242 WRKRVTALASEYPDVELSHMYVDNAAMQLVRDPKQFDTIVTNNIFGDILSDEASMITGSI 301

Query: 302 GMLPSASLRESGPGLFEPIHGSAPDIAGKDKANPLATVLSAAMLLKYGLGEENAAKRVEV 361
           GMLPSASL +SGPGLFEPIHGSAPDIAG+DKANPLAT+LSAAMLLKYGLGEE AAKR+E 
Sbjct: 302 GMLPSASLSDSGPGLFEPIHGSAPDIAGQDKANPLATILSAAMLLKYGLGEEKAAKRIED 361

Query: 362 AVLDTLDRGFRTGDIYSAGCKLVGCKEMGDELLKSIESYEPATV 406
           AVL  L+ GFRTGDIYSAG KLVGCKEMG+E+LKS++S  PA+V
Sbjct: 362 AVLVALNNGFRTGDIYSAGTKLVGCKEMGEEVLKSVDSQVPASV 405

[Close]

BLAST of MS016248 vs. ExPASy Swiss-Prot
Match: Q9FMT1 (3-isopropylmalate dehydrogenase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IMDH1 PE=1 SV=1)

HSP 1 Score: 609.0 bits (1569), Expect = 3.9e-173
Identity = 294/377 (77.98%), Postives = 339/377 (89.92%), Query Frame = 0


Query: 29  FRVSCSAGTSPSRRYSIALLPGDGVGPEVIAVAKDVLNFAATLEGIGFDYKEMPVGGAAL 88
           +R+ C+A +   +RY+IALLPGDG+GPEVI+VAK+VL  A +LEG+ FD+KEMPVGGAAL
Sbjct: 33  YRIRCAAASPGKKRYNIALLPGDGIGPEVISVAKNVLQKAGSLEGLEFDFKEMPVGGAAL 92

Query: 89  DLTGVPLPDETLLAAKQSDAVLFGAIGGYKWDDNPKHLKPETGLLQLRKGLEVFANLRPA 148
           DL GVPLP+ET  AAK SDA+L GAIGGYKWD N KHL+PE  L  LR+ L+VFANLRPA
Sbjct: 93  DLVGVPLPEETFTAAKLSDAILLGAIGGYKWDKNEKHLRPEMALFYLRRDLKVFANLRPA 152

Query: 149 TVFPQLVDASTLKKEVAEGVDLMVIRELTGGIYFGQPRGFKTSENGEEIGFNTEVYATHE 208
           TV PQLVDASTLKKEVAEGVD+M++RELTGGIYFG+PRG   +ENGEE+G +TE+YA HE
Sbjct: 153 TVLPQLVDASTLKKEVAEGVDMMIVRELTGGIYFGEPRGITINENGEEVGVSTEIYAAHE 212

Query: 209 IDRVARVAFENARKRQGRVCSVDKANVLEASMLWRKRVIAIAAEYPDIELSHMYVDNAAM 268
           IDR+ARVAFE ARKR+G++CSVDKANVL+AS+LWRKRV A+A+EYPD+ELSHMYVDNAAM
Sbjct: 213 IDRIARVAFETARKRRGKLCSVDKANVLDASILWRKRVTALASEYPDVELSHMYVDNAAM 272

Query: 269 QLIRNPKQFDTILTNNIFGDILSDAASMITGSIGMLPSASLRESGPGLFEPIHGSAPDIA 328
           QLIR+PKQFDTI+TNNIFGDILSD ASMITGSIGMLPSASL ESGPGLFEPIHGSAPDIA
Sbjct: 273 QLIRDPKQFDTIVTNNIFGDILSDEASMITGSIGMLPSASLGESGPGLFEPIHGSAPDIA 332

Query: 329 GKDKANPLATVLSAAMLLKYGLGEENAAKRVEVAVLDTLDRGFRTGDIYSAGCKLVGCKE 388
           G+DKANPLAT+LSAAMLLKYGLGEE AAKR+E AV+D L++GFRTGDIYS G KLVGCKE
Sbjct: 333 GQDKANPLATILSAAMLLKYGLGEEKAAKRIEDAVVDALNKGFRTGDIYSPGNKLVGCKE 392

Query: 389 MGDELLKSIESYEPATV 406
           MG+E+LKS+ES  PATV
Sbjct: 393 MGEEVLKSVESKVPATV 409

[Close]

BLAST of MS016248 vs. ExPASy Swiss-Prot
Match: Q9SA14 (3-isopropylmalate dehydrogenase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IMDH3 PE=1 SV=2)

HSP 1 Score: 600.9 bits (1548), Expect = 1.1e-170
Identity = 287/374 (76.74%), Postives = 335/374 (89.57%), Query Frame = 0


Query: 29  FRVSCSAGTSPSRRYSIALLPGDGVGPEVIAVAKDVLNFAATLEGIGFDYKEMPVGGAAL 88
           +R+ C+A +   +RY+I LLPGDG+GPEVI+VAK+VL  A  L+G+ FD++EMP GGAAL
Sbjct: 30  YRIRCAAASPVKKRYNITLLPGDGIGPEVISVAKNVLQKAGFLQGLEFDFQEMPFGGAAL 89

Query: 89  DLTGVPLPDETLLAAKQSDAVLFGAIGGYKWDDNPKHLKPETGLLQLRKGLEVFANLRPA 148
           DL GVPLP+ET  AAKQSDA+L GAIGGYKWD N KHL+PE GLL +R+ L VFANLRPA
Sbjct: 90  DLVGVPLPEETSTAAKQSDAILLGAIGGYKWDKNEKHLRPEMGLLNIRRDLNVFANLRPA 149

Query: 149 TVFPQLVDASTLKKEVAEGVDLMVIRELTGGIYFGQPRGFKTSENGEEIGFNTEVYATHE 208
           TV PQLVDASTLKKEVA+GVD+M++RELTGGIYFG+PRG   +ENGEE+GFNTE+YA HE
Sbjct: 150 TVLPQLVDASTLKKEVAQGVDMMIVRELTGGIYFGEPRGITINENGEEVGFNTEIYAAHE 209

Query: 209 IDRVARVAFENARKRQGRVCSVDKANVLEASMLWRKRVIAIAAEYPDIELSHMYVDNAAM 268
           IDR+ARVAFE ARKR+G++CSVDKANVL+AS+LWRKRV A+A+EYPD+ELSHMYVDNAAM
Sbjct: 210 IDRIARVAFETARKRRGKLCSVDKANVLDASILWRKRVTALASEYPDVELSHMYVDNAAM 269

Query: 269 QLIRNPKQFDTILTNNIFGDILSDAASMITGSIGMLPSASLRESGPGLFEPIHGSAPDIA 328
           QL+R+PKQFDTI+TNNIFGDILSD ASMITGSIGMLPSASL ESGPGLFEPIHGSAPDIA
Sbjct: 270 QLVRDPKQFDTIVTNNIFGDILSDEASMITGSIGMLPSASLGESGPGLFEPIHGSAPDIA 329

Query: 329 GKDKANPLATVLSAAMLLKYGLGEENAAKRVEVAVLDTLDRGFRTGDIYSAGCKLVGCKE 388
           G+DKANPLAT+LSAAMLLKYGLGEE AAK +E AV+D L++GFRTGDIYS G KLVGCKE
Sbjct: 330 GQDKANPLATILSAAMLLKYGLGEEKAAKMIEDAVVDALNKGFRTGDIYSPGNKLVGCKE 389

Query: 389 MGDELLKSIESYEP 403
           MG+E+LKS++S  P
Sbjct: 390 MGEEVLKSVDSKVP 403

[Close]

BLAST of MS016248 vs. ExPASy Swiss-Prot
Match: Q3M8T9 (3-isopropylmalate dehydrogenase OS=Trichormus variabilis (strain ATCC 29413 / PCC 7937) OX=240292 GN=leuB PE=3 SV=1)

HSP 1 Score: 494.2 bits (1271), Expect = 1.4e-138
Identity = 237/359 (66.02%), Postives = 287/359 (79.94%), Query Frame = 0


Query: 40  SRRYSIALLPGDGVGPEVIAVAKDVLNFAATLEGIGFDYKEMPVGGAALDLTGVPLPDET 99
           ++ Y I LLPGDG+GPE++AVA DVL        I FD++E  +GGAA+D TG PLP  T
Sbjct: 2   TQNYRITLLPGDGIGPEIMAVAVDVLKVVGQQFDIQFDFQEALIGGAAIDATGEPLPSAT 61

Query: 100 LLAAKQSDAVLFGAIGGYKWDDNPKHLKPETGLLQLRKGLEVFANLRPATVFPQLVDAST 159
           L   +QSDAVL  AIGGYKWD  P H +PE GLL LR GLE+FANLRPA + PQL+DAST
Sbjct: 62  LDTCRQSDAVLLAAIGGYKWDSLPPHQRPEGGLLGLRAGLELFANLRPAQILPQLIDAST 121

Query: 160 LKKEVAEGVDLMVIRELTGGIYFGQPRGFKTSENGEEIGFNTEVYATHEIDRVARVAFEN 219
           LK+EV EGVD+MV+RELTGGIYFG+PRG  T+E GE+ G NT VY   EIDR+ R+AFE 
Sbjct: 122 LKREVVEGVDIMVVRELTGGIYFGKPRGIFTTETGEKRGVNTMVYTESEIDRIGRIAFET 181

Query: 220 ARKRQGRVCSVDKANVLEASMLWRKRVIAIAAEYPDIELSHMYVDNAAMQLIRNPKQFDT 279
           ARKR+G++CSVDKANVL+ S LWR R+  ++ EYPD+ELSH+YVDNAAMQL+R PKQFDT
Sbjct: 182 ARKRRGKLCSVDKANVLDVSQLWRDRITKLSQEYPDVELSHLYVDNAAMQLVRAPKQFDT 241

Query: 280 ILTNNIFGDILSDAASMITGSIGMLPSASLRESGPGLFEPIHGSAPDIAGKDKANPLATV 339
           I+T N+FGDILSDAA+M+TGSIGMLPSASL  SGPG+FEP+HGSAPDIAG DKANPLA V
Sbjct: 242 IVTGNLFGDILSDAAAMLTGSIGMLPSASLGASGPGVFEPVHGSAPDIAGLDKANPLAQV 301

Query: 340 LSAAMLLKYGLGEENAAKRVEVAVLDTLDRGFRTGDIYSAGCKLVGCKEMGDELLKSIE 399
           LSAAM+L+Y L +  AA ++E AVL  L++G RTGDI S G  L+GC+ MGD L+K++E
Sbjct: 302 LSAAMMLRYALNQPQAADKIEQAVLQVLEQGDRTGDIMSVGMNLLGCRAMGDSLIKALE 360

[Close]

BLAST of MS016248 vs. ExPASy TrEMBL
Match: A0A6J1DGT0 (3-isopropylmalate dehydrogenase OS=Momordica charantia OX=3673 GN=LOC111020932 PE=3 SV=1)

HSP 1 Score: 798.9 bits (2062), Expect = 9.9e-228
Identity = 405/405 (100.00%), Postives = 405/405 (100.00%), Query Frame = 0


Query: 1   AAAALHLNAKPVNLFSRSITVSKPSPKLFRVSCSAGTSPSRRYSIALLPGDGVGPEVIAV 60
           AAAALHLNAKPVNLFSRSITVSKPSPKLFRVSCSAGTSPSRRYSIALLPGDGVGPEVIAV
Sbjct: 2   AAAALHLNAKPVNLFSRSITVSKPSPKLFRVSCSAGTSPSRRYSIALLPGDGVGPEVIAV 61

Query: 61  AKDVLNFAATLEGIGFDYKEMPVGGAALDLTGVPLPDETLLAAKQSDAVLFGAIGGYKWD 120
           AKDVLNFAATLEGIGFDYKEMPVGGAALDLTGVPLPDETLLAAKQSDAVLFGAIGGYKWD
Sbjct: 62  AKDVLNFAATLEGIGFDYKEMPVGGAALDLTGVPLPDETLLAAKQSDAVLFGAIGGYKWD 121

Query: 121 DNPKHLKPETGLLQLRKGLEVFANLRPATVFPQLVDASTLKKEVAEGVDLMVIRELTGGI 180
           DNPKHLKPETGLLQLRKGLEVFANLRPATVFPQLVDASTLKKEVAEGVDLMVIRELTGGI
Sbjct: 122 DNPKHLKPETGLLQLRKGLEVFANLRPATVFPQLVDASTLKKEVAEGVDLMVIRELTGGI 181

Query: 181 YFGQPRGFKTSENGEEIGFNTEVYATHEIDRVARVAFENARKRQGRVCSVDKANVLEASM 240
           YFGQPRGFKTSENGEEIGFNTEVYATHEIDRVARVAFENARKRQGRVCSVDKANVLEASM
Sbjct: 182 YFGQPRGFKTSENGEEIGFNTEVYATHEIDRVARVAFENARKRQGRVCSVDKANVLEASM 241

Query: 241 LWRKRVIAIAAEYPDIELSHMYVDNAAMQLIRNPKQFDTILTNNIFGDILSDAASMITGS 300
           LWRKRVIAIAAEYPDIELSHMYVDNAAMQLIRNPKQFDTILTNNIFGDILSDAASMITGS
Sbjct: 242 LWRKRVIAIAAEYPDIELSHMYVDNAAMQLIRNPKQFDTILTNNIFGDILSDAASMITGS 301

Query: 301 IGMLPSASLRESGPGLFEPIHGSAPDIAGKDKANPLATVLSAAMLLKYGLGEENAAKRVE 360
           IGMLPSASLRESGPGLFEPIHGSAPDIAGKDKANPLATVLSAAMLLKYGLGEENAAKRVE
Sbjct: 302 IGMLPSASLRESGPGLFEPIHGSAPDIAGKDKANPLATVLSAAMLLKYGLGEENAAKRVE 361

Query: 361 VAVLDTLDRGFRTGDIYSAGCKLVGCKEMGDELLKSIESYEPATV 406
           VAVLDTLDRGFRTGDIYSAGCKLVGCKEMGDELLKSIESYEPATV
Sbjct: 362 VAVLDTLDRGFRTGDIYSAGCKLVGCKEMGDELLKSIESYEPATV 406

[Close]

BLAST of MS016248 vs. ExPASy TrEMBL
Match: A0A6J1G9F4 (3-isopropylmalate dehydrogenase OS=Cucurbita moschata OX=3662 GN=LOC111452151 PE=3 SV=1)

HSP 1 Score: 748.8 bits (1932), Expect = 1.2e-212
Identity = 374/405 (92.35%), Postives = 394/405 (97.28%), Query Frame = 0


Query: 1   AAAALHLNAKPVNLFSRSITVSKPSPKLFRVSCSAGTSPSRRYSIALLPGDGVGPEVIAV 60
           AAA LHLNAKP N FS SI++SK SPKLFRV+CSAGTSPSRR++IALLPGDGVGPEVIAV
Sbjct: 3   AAATLHLNAKPSNFFSPSISLSKTSPKLFRVTCSAGTSPSRRFNIALLPGDGVGPEVIAV 62

Query: 61  AKDVLNFAATLEGIGFDYKEMPVGGAALDLTGVPLPDETLLAAKQSDAVLFGAIGGYKWD 120
           AKDVLN AA+LEGIGFDYKEMPVGGAALDLTGVPLPDETL+AAKQSDAVLFGAIGGYKWD
Sbjct: 63  AKDVLNLAASLEGIGFDYKEMPVGGAALDLTGVPLPDETLIAAKQSDAVLFGAIGGYKWD 122

Query: 121 DNPKHLKPETGLLQLRKGLEVFANLRPATVFPQLVDASTLKKEVAEGVDLMVIRELTGGI 180
           DNPKHLKPETGLLQLRKGLEVFANLRPATVFPQLV+ASTLK+EVAEGVDLMV+RELTGGI
Sbjct: 123 DNPKHLKPETGLLQLRKGLEVFANLRPATVFPQLVNASTLKREVAEGVDLMVVRELTGGI 182

Query: 181 YFGQPRGFKTSENGEEIGFNTEVYATHEIDRVARVAFENARKRQGRVCSVDKANVLEASM 240
           YFGQPRGFKT+ENGEEIGFNTEVYA HEIDRVARVAFENARKRQGRVCSVDKANVLEASM
Sbjct: 183 YFGQPRGFKTTENGEEIGFNTEVYAAHEIDRVARVAFENARKRQGRVCSVDKANVLEASM 242

Query: 241 LWRKRVIAIAAEYPDIELSHMYVDNAAMQLIRNPKQFDTILTNNIFGDILSDAASMITGS 300
           LWRKRV AIAAEYPD+ELSHMYVDNAAMQLIRNPKQFDTILTNNIFGDILSDAASMITGS
Sbjct: 243 LWRKRVTAIAAEYPDVELSHMYVDNAAMQLIRNPKQFDTILTNNIFGDILSDAASMITGS 302

Query: 301 IGMLPSASLRESGPGLFEPIHGSAPDIAGKDKANPLATVLSAAMLLKYGLGEENAAKRVE 360
           IGMLPSASLRESGPGLFEPIHGSAPDIAG+DKANPLATVLSAAMLLKYG+GEE+AAKR+E
Sbjct: 303 IGMLPSASLRESGPGLFEPIHGSAPDIAGQDKANPLATVLSAAMLLKYGIGEESAAKRIE 362

Query: 361 VAVLDTLDRGFRTGDIYSAGCKLVGCKEMGDELLKSIESYEPATV 406
            AVLDTL+RGFRTGDIYSAGCKLVGCK MG+ELLKSIES++ ATV
Sbjct: 363 AAVLDTLERGFRTGDIYSAGCKLVGCKAMGEELLKSIESHKLATV 407

[Close]

BLAST of MS016248 vs. ExPASy TrEMBL
Match: A0A6J1KE29 (3-isopropylmalate dehydrogenase OS=Cucurbita maxima OX=3661 GN=LOC111493011 PE=3 SV=1)

HSP 1 Score: 748.4 bits (1931), Expect = 1.5e-212
Identity = 375/405 (92.59%), Postives = 394/405 (97.28%), Query Frame = 0


Query: 1   AAAALHLNAKPVNLFSRSITVSKPSPKLFRVSCSAGTSPSRRYSIALLPGDGVGPEVIAV 60
           AAA LHLNAKP N FS SI++SK SPKLFRV+CSAGTSPSRR++IALLPGDGVGPEVIAV
Sbjct: 7   AAATLHLNAKPSNFFSPSISLSKTSPKLFRVTCSAGTSPSRRFNIALLPGDGVGPEVIAV 66

Query: 61  AKDVLNFAATLEGIGFDYKEMPVGGAALDLTGVPLPDETLLAAKQSDAVLFGAIGGYKWD 120
           AKDVLN AA+LEGIGFDYKEMPVGGAALDLTGVPLPDETL+AAKQSDAVLFGAIGGYKWD
Sbjct: 67  AKDVLNLAASLEGIGFDYKEMPVGGAALDLTGVPLPDETLMAAKQSDAVLFGAIGGYKWD 126

Query: 121 DNPKHLKPETGLLQLRKGLEVFANLRPATVFPQLVDASTLKKEVAEGVDLMVIRELTGGI 180
           DNPKHLKPETGLLQLRKGLEVFANLRPATVFPQLV+ASTLK+EVAEGVDLMV+RELTGGI
Sbjct: 127 DNPKHLKPETGLLQLRKGLEVFANLRPATVFPQLVNASTLKREVAEGVDLMVVRELTGGI 186

Query: 181 YFGQPRGFKTSENGEEIGFNTEVYATHEIDRVARVAFENARKRQGRVCSVDKANVLEASM 240
           YFGQPRGFKTSE+GEEIGFNTEVYA HEIDRVARVAFENARKRQGRVCSVDKANVLEASM
Sbjct: 187 YFGQPRGFKTSEDGEEIGFNTEVYAAHEIDRVARVAFENARKRQGRVCSVDKANVLEASM 246

Query: 241 LWRKRVIAIAAEYPDIELSHMYVDNAAMQLIRNPKQFDTILTNNIFGDILSDAASMITGS 300
           LWRKRV AIAAEYPDIELSHMYVDNAAMQLIRNPKQFDTILTNNIFGDILSDAASMITGS
Sbjct: 247 LWRKRVTAIAAEYPDIELSHMYVDNAAMQLIRNPKQFDTILTNNIFGDILSDAASMITGS 306

Query: 301 IGMLPSASLRESGPGLFEPIHGSAPDIAGKDKANPLATVLSAAMLLKYGLGEENAAKRVE 360
           IGMLPSASLRESGPGLFEPIHGSAPDIAG+DKANPLATVLSAAMLLKYG+GEE+AAKR+E
Sbjct: 307 IGMLPSASLRESGPGLFEPIHGSAPDIAGQDKANPLATVLSAAMLLKYGIGEESAAKRIE 366

Query: 361 VAVLDTLDRGFRTGDIYSAGCKLVGCKEMGDELLKSIESYEPATV 406
            AVLDTL+RGFRTGDIYSAGCKLVGCK MG+ELLKSIES++ ATV
Sbjct: 367 AAVLDTLERGFRTGDIYSAGCKLVGCKAMGEELLKSIESHKLATV 411

[Close]

BLAST of MS016248 vs. ExPASy TrEMBL
Match: A0A1S3BZA0 (3-isopropylmalate dehydrogenase OS=Cucumis melo OX=3656 GN=LOC103494678 PE=3 SV=1)

HSP 1 Score: 734.6 bits (1895), Expect = 2.3e-208
Identity = 369/404 (91.34%), Postives = 385/404 (95.30%), Query Frame = 0


Query: 2   AAALHLNAKPVNLFSRSITVSKPSPKLFRVSCSAGTSPSRRYSIALLPGDGVGPEVIAVA 61
           AAALHLNAKP NL S SI + K SP  FRV CSAGTSPSRRY+IALLPGDG+GPEVIAVA
Sbjct: 2   AAALHLNAKPFNLCSPSIRLPKSSPSPFRVKCSAGTSPSRRYNIALLPGDGIGPEVIAVA 61

Query: 62  KDVLNFAATLEGIGFDYKEMPVGGAALDLTGVPLPDETLLAAKQSDAVLFGAIGGYKWDD 121
           KDVLNFAA+LEGIGFDYKEMPVGGAALDLTGVPLPDETLLAAK+SDA+LFGAIGGYKWDD
Sbjct: 62  KDVLNFAASLEGIGFDYKEMPVGGAALDLTGVPLPDETLLAAKESDAILFGAIGGYKWDD 121

Query: 122 NPKHLKPETGLLQLRKGLEVFANLRPATVFPQLVDASTLKKEVAEGVDLMVIRELTGGIY 181
           NPKHLKPETGLLQLRKGL VFANLRPATVFPQLVDASTLK+EVAEGVDLMV+RELTGGIY
Sbjct: 122 NPKHLKPETGLLQLRKGLGVFANLRPATVFPQLVDASTLKREVAEGVDLMVVRELTGGIY 181

Query: 182 FGQPRGFKTSENGEEIGFNTEVYATHEIDRVARVAFENARKRQGRVCSVDKANVLEASML 241
           FGQPRGFKTSENGEEIGFNTEVYA  EIDRVARVAF NARKRQGRVCSVDKANVLEAS+L
Sbjct: 182 FGQPRGFKTSENGEEIGFNTEVYAAQEIDRVARVAFNNARKRQGRVCSVDKANVLEASIL 241

Query: 242 WRKRVIAIAAEYPDIELSHMYVDNAAMQLIRNPKQFDTILTNNIFGDILSDAASMITGSI 301
           WRKRV AIAAEYPD+ELSHMYVDNAAMQLIR PKQFDTILTNNIFGDILSDAASMITGSI
Sbjct: 242 WRKRVTAIAAEYPDVELSHMYVDNAAMQLIREPKQFDTILTNNIFGDILSDAASMITGSI 301

Query: 302 GMLPSASLRESGPGLFEPIHGSAPDIAGKDKANPLATVLSAAMLLKYGLGEENAAKRVEV 361
           GMLPSASLRESGPGLFEPIHGSAPDIAG+DKANPLATVLSAAMLLKYGLGEENAAKR+E 
Sbjct: 302 GMLPSASLRESGPGLFEPIHGSAPDIAGQDKANPLATVLSAAMLLKYGLGEENAAKRIEA 361

Query: 362 AVLDTLDRGFRTGDIYSAGCKLVGCKEMGDELLKSIESYEPATV 406
           AVLDTLD+GFRTGDIYSAGCK VGC EMG+ELLKSIES++ ATV
Sbjct: 362 AVLDTLDKGFRTGDIYSAGCKRVGCIEMGEELLKSIESHKLATV 405

[Close]

BLAST of MS016248 vs. ExPASy TrEMBL
Match: A0A0A0KTH3 (3-isopropylmalate dehydrogenase OS=Cucumis sativus OX=3659 GN=Csa_4G006270 PE=3 SV=1)

HSP 1 Score: 729.2 bits (1881), Expect = 9.6e-207
Identity = 366/404 (90.59%), Postives = 384/404 (95.05%), Query Frame = 0


Query: 2   AAALHLNAKPVNLFSRSITVSKPSPKLFRVSCSAGTSPSRRYSIALLPGDGVGPEVIAVA 61
           AAALHLNAKP +L S SI++ K SP  FRV CSAGTSPSRRY+IALLPGDG+GPEVIAVA
Sbjct: 2   AAALHLNAKPFSLCSPSISLPKISPSSFRVKCSAGTSPSRRYNIALLPGDGIGPEVIAVA 61

Query: 62  KDVLNFAATLEGIGFDYKEMPVGGAALDLTGVPLPDETLLAAKQSDAVLFGAIGGYKWDD 121
           KDVLNFAA+LEGIGFDYKEMP+GGAALDLTGVPLPDETLLAAK SDA+LFGAIGGYKWDD
Sbjct: 62  KDVLNFAASLEGIGFDYKEMPMGGAALDLTGVPLPDETLLAAKDSDAILFGAIGGYKWDD 121

Query: 122 NPKHLKPETGLLQLRKGLEVFANLRPATVFPQLVDASTLKKEVAEGVDLMVIRELTGGIY 181
           NPKHLKPETGLLQLRKGL VFANLRPA VFPQLV+ASTLK+EVAEGVDLMV+RELTGGIY
Sbjct: 122 NPKHLKPETGLLQLRKGLGVFANLRPAIVFPQLVEASTLKREVAEGVDLMVVRELTGGIY 181

Query: 182 FGQPRGFKTSENGEEIGFNTEVYATHEIDRVARVAFENARKRQGRVCSVDKANVLEASML 241
           FGQPRGFKT ENGEEIGFNTEVYA  EIDRVARVAF NARKRQGRVCSVDKANVLEAS+L
Sbjct: 182 FGQPRGFKTGENGEEIGFNTEVYAAQEIDRVARVAFNNARKRQGRVCSVDKANVLEASIL 241

Query: 242 WRKRVIAIAAEYPDIELSHMYVDNAAMQLIRNPKQFDTILTNNIFGDILSDAASMITGSI 301
           WRKRV AIAAEYPDIELSHMYVDNAAMQLIR PKQFDTILTNNIFGDILSDAASMITGSI
Sbjct: 242 WRKRVTAIAAEYPDIELSHMYVDNAAMQLIREPKQFDTILTNNIFGDILSDAASMITGSI 301

Query: 302 GMLPSASLRESGPGLFEPIHGSAPDIAGKDKANPLATVLSAAMLLKYGLGEENAAKRVEV 361
           GMLPSASLRESGPGLFEPIHGSAPDIAG+DKANPLATVLSAAMLLKYGLGEENAAKR+E 
Sbjct: 302 GMLPSASLRESGPGLFEPIHGSAPDIAGQDKANPLATVLSAAMLLKYGLGEENAAKRIEA 361

Query: 362 AVLDTLDRGFRTGDIYSAGCKLVGCKEMGDELLKSIESYEPATV 406
           AVLDTLD+GFRTGDIYSAGCK VGCKEMG+ELLKSIES++ ATV
Sbjct: 362 AVLDTLDKGFRTGDIYSAGCKRVGCKEMGEELLKSIESHKLATV 405

[Close]

BLAST of MS016248 vs. TAIR 10
Match: AT1G80560.1 (isopropylmalate dehydrogenase 2 )

HSP 1 Score: 626.7 bits (1615), Expect = 1.3e-179
Identity = 306/404 (75.74%), Postives = 357/404 (88.37%), Query Frame = 0


Query: 2   AAALHLNAKPVNLFSRSITVSKPSPKLFRVSCSAGTSPSRRYSIALLPGDGVGPEVIAVA 61
           AAAL  N + V + +    VSK S   FRV C+  +   +RY+I LLPGDG+GPEV+++A
Sbjct: 2   AAALQTNIRTVKVPATFRAVSKQSLAPFRVRCAVASPGKKRYTITLLPGDGIGPEVVSIA 61

Query: 62  KDVLNFAATLEGIGFDYKEMPVGGAALDLTGVPLPDETLLAAKQSDAVLFGAIGGYKWDD 121
           K+VL  A +LEG+ F+++EMP+GGAALDL GVPLP+ET+ AAK+SDAVL GAIGGYKWD+
Sbjct: 62  KNVLQQAGSLEGVEFNFREMPIGGAALDLVGVPLPEETISAAKESDAVLLGAIGGYKWDN 121

Query: 122 NPKHLKPETGLLQLRKGLEVFANLRPATVFPQLVDASTLKKEVAEGVDLMVIRELTGGIY 181
           N KHL+PE GLLQ+R  L+VFANLRPATV PQLVDASTLK+EVAEGVDLMV+RELTGGIY
Sbjct: 122 NEKHLRPEKGLLQIRAALKVFANLRPATVLPQLVDASTLKREVAEGVDLMVVRELTGGIY 181

Query: 182 FGQPRGFKTSENGEEIGFNTEVYATHEIDRVARVAFENARKRQGRVCSVDKANVLEASML 241
           FG+PRG KT+ENGEE+GFNTEVYA HEIDR+ARVAFE ARKR+G++CSVDKANVLEAS+L
Sbjct: 182 FGEPRGIKTNENGEEVGFNTEVYAAHEIDRIARVAFETARKRRGKLCSVDKANVLEASIL 241

Query: 242 WRKRVIAIAAEYPDIELSHMYVDNAAMQLIRNPKQFDTILTNNIFGDILSDAASMITGSI 301
           WRKRV A+A+EYPD+ELSHMYVDNAAMQL+R+PKQFDTI+TNNIFGDILSD ASMITGSI
Sbjct: 242 WRKRVTALASEYPDVELSHMYVDNAAMQLVRDPKQFDTIVTNNIFGDILSDEASMITGSI 301

Query: 302 GMLPSASLRESGPGLFEPIHGSAPDIAGKDKANPLATVLSAAMLLKYGLGEENAAKRVEV 361
           GMLPSASL +SGPGLFEPIHGSAPDIAG+DKANPLAT+LSAAMLLKYGLGEE AAKR+E 
Sbjct: 302 GMLPSASLSDSGPGLFEPIHGSAPDIAGQDKANPLATILSAAMLLKYGLGEEKAAKRIED 361

Query: 362 AVLDTLDRGFRTGDIYSAGCKLVGCKEMGDELLKSIESYEPATV 406
           AVL  L+ GFRTGDIYSAG KLVGCKEMG+E+LKS++S  PA+V
Sbjct: 362 AVLVALNNGFRTGDIYSAGTKLVGCKEMGEEVLKSVDSQVPASV 405

[Close]

BLAST of MS016248 vs. TAIR 10
Match: AT5G14200.1 (isopropylmalate dehydrogenase 1 )

HSP 1 Score: 609.0 bits (1569), Expect = 2.8e-174
Identity = 294/377 (77.98%), Postives = 339/377 (89.92%), Query Frame = 0


Query: 29  FRVSCSAGTSPSRRYSIALLPGDGVGPEVIAVAKDVLNFAATLEGIGFDYKEMPVGGAAL 88
           +R+ C+A +   +RY+IALLPGDG+GPEVI+VAK+VL  A +LEG+ FD+KEMPVGGAAL
Sbjct: 33  YRIRCAAASPGKKRYNIALLPGDGIGPEVISVAKNVLQKAGSLEGLEFDFKEMPVGGAAL 92

Query: 89  DLTGVPLPDETLLAAKQSDAVLFGAIGGYKWDDNPKHLKPETGLLQLRKGLEVFANLRPA 148
           DL GVPLP+ET  AAK SDA+L GAIGGYKWD N KHL+PE  L  LR+ L+VFANLRPA
Sbjct: 93  DLVGVPLPEETFTAAKLSDAILLGAIGGYKWDKNEKHLRPEMALFYLRRDLKVFANLRPA 152

Query: 149 TVFPQLVDASTLKKEVAEGVDLMVIRELTGGIYFGQPRGFKTSENGEEIGFNTEVYATHE 208
           TV PQLVDASTLKKEVAEGVD+M++RELTGGIYFG+PRG   +ENGEE+G +TE+YA HE
Sbjct: 153 TVLPQLVDASTLKKEVAEGVDMMIVRELTGGIYFGEPRGITINENGEEVGVSTEIYAAHE 212

Query: 209 IDRVARVAFENARKRQGRVCSVDKANVLEASMLWRKRVIAIAAEYPDIELSHMYVDNAAM 268
           IDR+ARVAFE ARKR+G++CSVDKANVL+AS+LWRKRV A+A+EYPD+ELSHMYVDNAAM
Sbjct: 213 IDRIARVAFETARKRRGKLCSVDKANVLDASILWRKRVTALASEYPDVELSHMYVDNAAM 272

Query: 269 QLIRNPKQFDTILTNNIFGDILSDAASMITGSIGMLPSASLRESGPGLFEPIHGSAPDIA 328
           QLIR+PKQFDTI+TNNIFGDILSD ASMITGSIGMLPSASL ESGPGLFEPIHGSAPDIA
Sbjct: 273 QLIRDPKQFDTIVTNNIFGDILSDEASMITGSIGMLPSASLGESGPGLFEPIHGSAPDIA 332

Query: 329 GKDKANPLATVLSAAMLLKYGLGEENAAKRVEVAVLDTLDRGFRTGDIYSAGCKLVGCKE 388
           G+DKANPLAT+LSAAMLLKYGLGEE AAKR+E AV+D L++GFRTGDIYS G KLVGCKE
Sbjct: 333 GQDKANPLATILSAAMLLKYGLGEEKAAKRIEDAVVDALNKGFRTGDIYSPGNKLVGCKE 392

Query: 389 MGDELLKSIESYEPATV 406
           MG+E+LKS+ES  PATV
Sbjct: 393 MGEEVLKSVESKVPATV 409

[Close]

BLAST of MS016248 vs. TAIR 10
Match: AT1G31180.1 (isopropylmalate dehydrogenase 3 )

HSP 1 Score: 600.9 bits (1548), Expect = 7.6e-172
Identity = 287/374 (76.74%), Postives = 335/374 (89.57%), Query Frame = 0


Query: 29  FRVSCSAGTSPSRRYSIALLPGDGVGPEVIAVAKDVLNFAATLEGIGFDYKEMPVGGAAL 88
           +R+ C+A +   +RY+I LLPGDG+GPEVI+VAK+VL  A  L+G+ FD++EMP GGAAL
Sbjct: 30  YRIRCAAASPVKKRYNITLLPGDGIGPEVISVAKNVLQKAGFLQGLEFDFQEMPFGGAAL 89

Query: 89  DLTGVPLPDETLLAAKQSDAVLFGAIGGYKWDDNPKHLKPETGLLQLRKGLEVFANLRPA 148
           DL GVPLP+ET  AAKQSDA+L GAIGGYKWD N KHL+PE GLL +R+ L VFANLRPA
Sbjct: 90  DLVGVPLPEETSTAAKQSDAILLGAIGGYKWDKNEKHLRPEMGLLNIRRDLNVFANLRPA 149

Query: 149 TVFPQLVDASTLKKEVAEGVDLMVIRELTGGIYFGQPRGFKTSENGEEIGFNTEVYATHE 208
           TV PQLVDASTLKKEVA+GVD+M++RELTGGIYFG+PRG   +ENGEE+GFNTE+YA HE
Sbjct: 150 TVLPQLVDASTLKKEVAQGVDMMIVRELTGGIYFGEPRGITINENGEEVGFNTEIYAAHE 209

Query: 209 IDRVARVAFENARKRQGRVCSVDKANVLEASMLWRKRVIAIAAEYPDIELSHMYVDNAAM 268
           IDR+ARVAFE ARKR+G++CSVDKANVL+AS+LWRKRV A+A+EYPD+ELSHMYVDNAAM
Sbjct: 210 IDRIARVAFETARKRRGKLCSVDKANVLDASILWRKRVTALASEYPDVELSHMYVDNAAM 269

Query: 269 QLIRNPKQFDTILTNNIFGDILSDAASMITGSIGMLPSASLRESGPGLFEPIHGSAPDIA 328
           QL+R+PKQFDTI+TNNIFGDILSD ASMITGSIGMLPSASL ESGPGLFEPIHGSAPDIA
Sbjct: 270 QLVRDPKQFDTIVTNNIFGDILSDEASMITGSIGMLPSASLGESGPGLFEPIHGSAPDIA 329

Query: 329 GKDKANPLATVLSAAMLLKYGLGEENAAKRVEVAVLDTLDRGFRTGDIYSAGCKLVGCKE 388
           G+DKANPLAT+LSAAMLLKYGLGEE AAK +E AV+D L++GFRTGDIYS G KLVGCKE
Sbjct: 330 GQDKANPLATILSAAMLLKYGLGEEKAAKMIEDAVVDALNKGFRTGDIYSPGNKLVGCKE 389

Query: 389 MGDELLKSIESYEP 403
           MG+E+LKS++S  P
Sbjct: 390 MGEEVLKSVDSKVP 403

[Close]

BLAST of MS016248 vs. TAIR 10
Match: AT5G14200.3 (isopropylmalate dehydrogenase 1 )

HSP 1 Score: 570.9 bits (1470), Expect = 8.4e-163
Identity = 275/352 (78.12%), Postives = 317/352 (90.06%), Query Frame = 0


Query: 29  FRVSCSAGTSPSRRYSIALLPGDGVGPEVIAVAKDVLNFAATLEGIGFDYKEMPVGGAAL 88
           +R+ C+A +   +RY+IALLPGDG+GPEVI+VAK+VL  A +LEG+ FD+KEMPVGGAAL
Sbjct: 33  YRIRCAAASPGKKRYNIALLPGDGIGPEVISVAKNVLQKAGSLEGLEFDFKEMPVGGAAL 92

Query: 89  DLTGVPLPDETLLAAKQSDAVLFGAIGGYKWDDNPKHLKPETGLLQLRKGLEVFANLRPA 148
           DL GVPLP+ET  AAK SDA+L GAIGGYKWD N KHL+PE  L  LR+ L+VFANLRPA
Sbjct: 93  DLVGVPLPEETFTAAKLSDAILLGAIGGYKWDKNEKHLRPEMALFYLRRDLKVFANLRPA 152

Query: 149 TVFPQLVDASTLKKEVAEGVDLMVIRELTGGIYFGQPRGFKTSENGEEIGFNTEVYATHE 208
           TV PQLVDASTLKKEVAEGVD+M++RELTGGIYFG+PRG   +ENGEE+G +TE+YA HE
Sbjct: 153 TVLPQLVDASTLKKEVAEGVDMMIVRELTGGIYFGEPRGITINENGEEVGVSTEIYAAHE 212

Query: 209 IDRVARVAFENARKRQGRVCSVDKANVLEASMLWRKRVIAIAAEYPDIELSHMYVDNAAM 268
           IDR+ARVAFE ARKR+G++CSVDKANVL+AS+LWRKRV A+A+EYPD+ELSHMYVDNAAM
Sbjct: 213 IDRIARVAFETARKRRGKLCSVDKANVLDASILWRKRVTALASEYPDVELSHMYVDNAAM 272

Query: 269 QLIRNPKQFDTILTNNIFGDILSDAASMITGSIGMLPSASLRESGPGLFEPIHGSAPDIA 328
           QLIR+PKQFDTI+TNNIFGDILSD ASMITGSIGMLPSASL ESGPGLFEPIHGSAPDIA
Sbjct: 273 QLIRDPKQFDTIVTNNIFGDILSDEASMITGSIGMLPSASLGESGPGLFEPIHGSAPDIA 332

Query: 329 GKDKANPLATVLSAAMLLKYGLGEENAAKRVEVAVLDTLDRGFRTGDIYSAG 381
           G+DKANPLAT+LSAAMLLKYGLGEE AAKR+E AV+D L++GFRTGDIYS G
Sbjct: 333 GQDKANPLATILSAAMLLKYGLGEEKAAKRIEDAVVDALNKGFRTGDIYSPG 384

[Close]

BLAST of MS016248 vs. TAIR 10
Match: AT5G14200.2 (isopropylmalate dehydrogenase 1 )

HSP 1 Score: 392.9 bits (1008), Expect = 3.1e-109
Identity = 186/257 (72.37%), Postives = 224/257 (87.16%), Query Frame = 0


Query: 29  FRVSCSAGTSPSRRYSIALLPGDGVGPEVIAVAKDVLNFAATLEGIGFDYKEMPVGGAAL 88
           +R+ C+A +   +RY+IALLPGDG+GPEVI+VAK+VL  A +LEG+ FD+KEMPVGGAAL
Sbjct: 33  YRIRCAAASPGKKRYNIALLPGDGIGPEVISVAKNVLQKAGSLEGLEFDFKEMPVGGAAL 92

Query: 89  DLTGVPLPDETLLAAKQSDAVLFGAIGGYKWDDNPKHLKPETGLLQLRKGLEVFANLRPA 148
           DL GVPLP+ET  AAK SDA+L GAIGGYKWD N KHL+PE  L  LR+ L+VFANLRPA
Sbjct: 93  DLVGVPLPEETFTAAKLSDAILLGAIGGYKWDKNEKHLRPEMALFYLRRDLKVFANLRPA 152

Query: 149 TVFPQLVDASTLKKEVAEGVDLMVIRELTGGIYFGQPRGFKTSENGEEIGFNTEVYATHE 208
           TV PQLVDASTLKKEVAEGVD+M++RELTGGIYFG+PRG   +ENGEE+G +TE+YA HE
Sbjct: 153 TVLPQLVDASTLKKEVAEGVDMMIVRELTGGIYFGEPRGITINENGEEVGVSTEIYAAHE 212

Query: 209 IDRVARVAFENARKRQGRVCSVDKANVLEASMLWRKRVIAIAAEYPDIELSHMYVDNAAM 268
           IDR+ARVAFE ARKR+G++CSVDKANVL+AS+LWRKRV A+A+EYPD+ELSHMYVDNAAM
Sbjct: 213 IDRIARVAFETARKRRGKLCSVDKANVLDASILWRKRVTALASEYPDVELSHMYVDNAAM 272

Query: 269 QLIRNPKQFDTILTNNI 286
           QLIR+PKQ   +LT ++
Sbjct: 273 QLIRDPKQ---VLTQSL 286

The following BLAST results are available for this feature:

BLAST of MS016248 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022153420.1	2.0e-227	100.00	3-isopropylmalate dehydrogenase 2, chloroplastic-like [Momordica charantia]	[more]
XP_023525901.1	8.3e-213	92.84	3-isopropylmalate dehydrogenase, chloroplastic-like [Cucurbita pepo subsp. pepo]	[more]
KAG6606821.1	1.1e-212	92.59	hypothetical protein SDJN03_00163, partial [Cucurbita argyrosperma subsp. sorori...	[more]
KAG7036527.1	1.1e-212	92.59	hypothetical protein SDJN02_00145, partial [Cucurbita argyrosperma subsp. argyro...	[more]
XP_022948488.1	2.4e-212	92.35	3-isopropylmalate dehydrogenase, chloroplastic-like [Cucurbita moschata]	[more]

BLAST of MS016248 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P29102	4.8e-179	76.60	3-isopropylmalate dehydrogenase, chloroplastic OS=Brassica napus OX=3708 PE=2 SV...	[more]
P93832	1.8e-178	75.74	3-isopropylmalate dehydrogenase 2, chloroplastic OS=Arabidopsis thaliana OX=3702...	[more]
Q9FMT1	3.9e-173	77.98	3-isopropylmalate dehydrogenase 1, chloroplastic OS=Arabidopsis thaliana OX=3702...	[more]
Q9SA14	1.1e-170	76.74	3-isopropylmalate dehydrogenase 3, chloroplastic OS=Arabidopsis thaliana OX=3702...	[more]
Q3M8T9	1.4e-138	66.02	3-isopropylmalate dehydrogenase OS=Trichormus variabilis (strain ATCC 29413 / PC...	[more]

BLAST of MS016248 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DGT0	9.9e-228	100.00	3-isopropylmalate dehydrogenase OS=Momordica charantia OX=3673 GN=LOC111020932 P...	[more]
A0A6J1G9F4	1.2e-212	92.35	3-isopropylmalate dehydrogenase OS=Cucurbita moschata OX=3662 GN=LOC111452151 PE...	[more]
A0A6J1KE29	1.5e-212	92.59	3-isopropylmalate dehydrogenase OS=Cucurbita maxima OX=3661 GN=LOC111493011 PE=3...	[more]
A0A1S3BZA0	2.3e-208	91.34	3-isopropylmalate dehydrogenase OS=Cucumis melo OX=3656 GN=LOC103494678 PE=3 SV=...	[more]
A0A0A0KTH3	9.6e-207	90.59	3-isopropylmalate dehydrogenase OS=Cucumis sativus OX=3659 GN=Csa_4G006270 PE=3 ...	[more]

BLAST of MS016248 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G80560.1	1.3e-179	75.74	isopropylmalate dehydrogenase 2	[more]
AT5G14200.1	2.8e-174	77.98	isopropylmalate dehydrogenase 1	[more]
AT1G31180.1	7.6e-172	76.74	isopropylmalate dehydrogenase 3	[more]
AT5G14200.3	8.4e-163	78.13	isopropylmalate dehydrogenase 1	[more]
AT5G14200.2	3.1e-109	72.37	isopropylmalate dehydrogenase 1	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR024084	Isopropylmalate dehydrogenase-like domain	SMART	SM01329	Iso_dh_2	coord: 44..393 e-value: 1.2E-192 score: 656.0
IPR024084	Isopropylmalate dehydrogenase-like domain	PFAM	PF00180	Iso_dh	coord: 45..392 e-value: 1.2E-135 score: 452.2
None	No IPR available	GENE3D	3.40.718.10	Isopropylmalate Dehydrogenase	coord: 32..400 e-value: 4.5E-133 score: 445.7
None	No IPR available	PANTHER	PTHR42979:SF7	3-ISOPROPYLMALATE DEHYDROGENASE	coord: 2..404
None	No IPR available	SUPERFAMILY	53659	Isocitrate/Isopropylmalate dehydrogenase-like	coord: 40..398
IPR004429	Isopropylmalate dehydrogenase	TIGRFAM	TIGR00169	TIGR00169	coord: 45..393 e-value: 2.9E-155 score: 514.5
IPR004429	Isopropylmalate dehydrogenase	PANTHER	PTHR42979	3-ISOPROPYLMALATE DEHYDROGENASE	coord: 2..404
IPR004429	Isopropylmalate dehydrogenase	HAMAP	MF_01033	LeuB_type1	coord: 43..397 score: 50.351288
IPR019818	Isocitrate/isopropylmalate dehydrogenase, conserved site	PROSITE	PS00470	IDH_IMDH	coord: 284..303

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS016248.1	MS016248.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009098	leucine biosynthetic process
molecular_function	GO:0003862	3-isopropylmalate dehydrogenase activity
molecular_function	GO:0000287	magnesium ion binding
molecular_function	GO:0051287	NAD binding
molecular_function	GO:0016616	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor