MS016234 (gene) Bitter gourd (TR) v1

Overview


Name	MS016234
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	aquaporin NIP6-1
Location	scaffold9_2: 1329964 .. 1332559 (+)
RNA-Seq Expression	MS016234
Synteny	MS016234

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGACACAGATCAGGAAGCTCCATCGACACCGGTGACGCCGGGGACACCTGGAGCTCCTCTTTTTGGAGGGATCAGGGCGGAACACCATGGAAATGGAAATGGTAAAAGATCTCTTCTCAAGAGCTGCATCTCTTTCAATGCCGATGATTGGACCAATGAAGAAAGAGGCTTATCCTCCAAGGTCTCTTGTTCATTGTCGCCTCCTCCTATCTCACTTGCAAGAAAGGTAATTCTCGAGAGATGAAAGAACAAATTTTAAGATCTATTTAGTAATATTACAGTTTCTTATTTCTTATTTTCTGTTTATCATTTTTCGTAGAATGAAAAAAAACTACATTTAGCATAACATCTTATTTATCCTTTTCACTAAAAGATGTACCTCAGTGTCAGTGATGGTTCCAGTGATGGTTCCTTATTTCAAGTTCTCTAACATTTTAAATGAAAAAAAAAAGTTACATGAAATCTATCTTTTCTCAAATAAAATATTCAAAATTATTACATTATATATATTTGTTAATATTTGCGATGCATTTAATAAATATAGAATAGTAAACTTATATTTTTATTAACATGATTGGAAAATTCAAGCAGATAATAAAAAGAAAATGTTTCTAAAAAAACAATAAATAGGAAAAAGAAATCAGACTGCGATGTATCACTCAAAACAAGAAACAAAAACATTATCAAATGGCCTCTTAACCCCACATCTCGTTATACCTAAAGGTGGGAGCAGAGTTCATCGCTACTCTGATGCTCATCTTTGCTGGAACCGCCACGGCCATTGTAAACCAGAAGACACAAGGAACCGAAACACTCATCGGCCTAGCAGCCTCGACCGGGCTGGCGGTGATGATTGTCATCTTGTCGACTGGCCACATCTCCGGAGCCCATCTGAACCCGGCCATCACCATTGCTTTTGCAGCACTAAAGCACTTTCCATGGAAGCATGTAGGCGCCATGATCTCCATGCACATCAATCTTCTGATGCTTAGAAATCTCTCTGAGTTGCTTGTTTTTGTTTTTGTTTTTGCAGGTTCCGGTGTATATTGGAGCTCAAATGGTGGCTTCTCTGTGTGCTTCTTTTGCATTGAAATGGATCTTTGATCCAATAATGGGGGGAGGAGTCACTGTTCCTTCAGTTGGATATGAGCAGGCTTTTGCTCTAGAGTTCATCATTAGCTTCAACCTCATGTTTGTCATCACCGCAGTCGCCACTGATACTCGAGCTGTGAGTTACTCCTTGTCCTTGCTTCTTTTGACTTTGAATTCCTGTTCTGAAAAGAAGAAATTTTGATATAAAAAAAATATATATATATATACTATCGGTTTAATTTCTGCCAACCTAAGTATAGCTCAACTAGTTACCTAGAAAAAATCACAAAGTGACATGGAGTTGGAAATGGCAGGTGGGAGATTTGGCGGGAATCGCGGTAGGAGCAACAGTCATGCTCAATGTCCTCATCGCCGGGTATGTCCAAAACAATCCCAATAAATTAATCACGGCCCTTTAATTTATTATTATTATTATTATTAAATTACTTCCTCAAACTTTTTAAATTATGTCTAATAGATCCTAATAAGTCTCTAAACTTTTAACGTTGATGTTGATGCTGTCTAATGTGTCCATAAACCTAAAAAAATATTTAGTAGAACTCCGAACTTTTAAAGTTAACCCGACATTTTTCATTTTTTTGTATCCCCTGAATTTTAAAAAATATCTAATAAGTCAGTTTCACCTAGTCTAAACCTACTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTTTTCTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCAAAAAATTTACTCAACCATTTTTTAGTACGTGGGAGAGTGAACCTTCAATATCAAGAAAGGTAATTGACCATTAGACCACCTCATCTAAAGTTTAAGAAATTGATTAACTTTGGGAATTTATAAACATGATGATATGCCATTAAAATACAGTTGATTAACTTTGGGAATTCTAAATAACCTTTTCACGTCATATGACTCAACCCGTAATAACTTCTCTAACTTGAAATAGATTGGCCAACTTAAGAAAAAATGTGGAACTTCTCTATAATGAAGTAAGAACCATAGTTAAAACAGATATAAACTAAGTGGCTCCTGTGTCTAAAACGAAACGGTTGGTAATCTAGAGCCTCTTATGAGCAACTTAACCTTCACTAAACAAGAAACAGACTAAAAGATTCTCATTTTGATTATGCTATTATAGGGAAACAACTGGAGCTTCAATGAATCCAGTGAGAACACTGGGGCCAGCCATAGTTACAAATAACTTTAAAGCCATATGGATTTACCTCACAGCTCCCATCCTAGGGACATTGTGTGGGGCTGGCATCTACACTGCAGTAAAGTTGCCTGACGAACATGGCGACGTGCATAGGAAGCCTTCTGCCGAGCGCAGCTTCCGAAGA

mRNA sequence

ATGGACACAGATCAGGAAGCTCCATCGACACCGGTGACGCCGGGGACACCTGGAGCTCCTCTTTTTGGAGGGATCAGGGCGGAACACCATGGAAATGGAAATGGTAAAAGATCTCTTCTCAAGAGCTGCATCTCTTTCAATGCCGATGATTGGACCAATGAAGAAAGAGGCTTATCCTCCAAGGTCTCTTGTTCATTGTCGCCTCCTCCTATCTCACTTGCAAGAAAGGTGGGAGCAGAGTTCATCGCTACTCTGATGCTCATCTTTGCTGGAACCGCCACGGCCATTGTAAACCAGAAGACACAAGGAACCGAAACACTCATCGGCCTAGCAGCCTCGACCGGGCTGGCGGTGATGATTGTCATCTTGTCGACTGGCCACATCTCCGGAGCCCATCTGAACCCGGCCATCACCATTGCTTTTGCAGCACTAAAGCACTTTCCATGGAAGCATGTTCCGGTGTATATTGGAGCTCAAATGGTGGCTTCTCTGTGTGCTTCTTTTGCATTGAAATGGATCTTTGATCCAATAATGGGGGGAGGAGTCACTGTTCCTTCAGTTGGATATGAGCAGGCTTTTGCTCTAGAGTTCATCATTAGCTTCAACCTCATGTTTGTCATCACCGCAGTCGCCACTGATACTCGAGCTGTGGGAGATTTGGCGGGAATCGCGGTAGGAGCAACAGTCATGCTCAATGTCCTCATCGCCGGGGAAACAACTGGAGCTTCAATGAATCCAGTGAGAACACTGGGGCCAGCCATAGTTACAAATAACTTTAAAGCCATATGGATTTACCTCACAGCTCCCATCCTAGGGACATTGTGTGGGGCTGGCATCTACACTGCAGTAAAGTTGCCTGACGAACATGGCGACGTGCATAGGAAGCCTTCTGCCGAGCGCAGCTTCCGAAGA

Coding sequence (CDS)

ATGGACACAGATCAGGAAGCTCCATCGACACCGGTGACGCCGGGGACACCTGGAGCTCCTCTTTTTGGAGGGATCAGGGCGGAACACCATGGAAATGGAAATGGTAAAAGATCTCTTCTCAAGAGCTGCATCTCTTTCAATGCCGATGATTGGACCAATGAAGAAAGAGGCTTATCCTCCAAGGTCTCTTGTTCATTGTCGCCTCCTCCTATCTCACTTGCAAGAAAGGTGGGAGCAGAGTTCATCGCTACTCTGATGCTCATCTTTGCTGGAACCGCCACGGCCATTGTAAACCAGAAGACACAAGGAACCGAAACACTCATCGGCCTAGCAGCCTCGACCGGGCTGGCGGTGATGATTGTCATCTTGTCGACTGGCCACATCTCCGGAGCCCATCTGAACCCGGCCATCACCATTGCTTTTGCAGCACTAAAGCACTTTCCATGGAAGCATGTTCCGGTGTATATTGGAGCTCAAATGGTGGCTTCTCTGTGTGCTTCTTTTGCATTGAAATGGATCTTTGATCCAATAATGGGGGGAGGAGTCACTGTTCCTTCAGTTGGATATGAGCAGGCTTTTGCTCTAGAGTTCATCATTAGCTTCAACCTCATGTTTGTCATCACCGCAGTCGCCACTGATACTCGAGCTGTGGGAGATTTGGCGGGAATCGCGGTAGGAGCAACAGTCATGCTCAATGTCCTCATCGCCGGGGAAACAACTGGAGCTTCAATGAATCCAGTGAGAACACTGGGGCCAGCCATAGTTACAAATAACTTTAAAGCCATATGGATTTACCTCACAGCTCCCATCCTAGGGACATTGTGTGGGGCTGGCATCTACACTGCAGTAAAGTTGCCTGACGAACATGGCGACGTGCATAGGAAGCCTTCTGCCGAGCGCAGCTTCCGAAGA

Protein sequence

MDTDQEAPSTPVTPGTPGAPLFGGIRAEHHGNGNGKRSLLKSCISFNADDWTNEERGLSSKVSCSLSPPPISLARKVGAEFIATLMLIFAGTATAIVNQKTQGTETLIGLAASTGLAVMIVILSTGHISGAHLNPAITIAFAALKHFPWKHVPVYIGAQMVASLCASFALKWIFDPIMGGGVTVPSVGYEQAFALEFIISFNLMFVITAVATDTRAVGDLAGIAVGATVMLNVLIAGETTGASMNPVRTLGPAIVTNNFKAIWIYLTAPILGTLCGAGIYTAVKLPDEHGDVHRKPSAERSFRR

Homology

[Close]

BLAST of MS016234 vs. NCBI nr
Match: XP_022153403.1 (aquaporin NIP6-1 [Momordica charantia])

HSP 1 Score: 576.6 bits (1485), Expect = 1.2e-160
Identity = 296/304 (97.37%), Postives = 299/304 (98.36%), Query Frame = 0


Query: 1   MDTDQEAPSTPVTPGTPGAPLFGGIRAEHHGNGNGKRSLLKSCISFNADDWTNEERGLSS 60
           MDTDQEAPS   TPGTPGAPLF GIRAEHHGNGNGKRSLLK+CISFNADDWTNEERGLSS
Sbjct: 1   MDTDQEAPS---TPGTPGAPLFEGIRAEHHGNGNGKRSLLKNCISFNADDWTNEERGLSS 60

Query: 61  KVSCSLSPPPISLARKVGAEFIATLMLIFAGTATAIVNQKTQGTETLIGLAASTGLAVMI 120
           KVSCSLSPPPISLARKVGAEFI+TLMLIFAGTATAIVNQKTQGTETLIGLAASTGLAVMI
Sbjct: 61  KVSCSLSPPPISLARKVGAEFISTLMLIFAGTATAIVNQKTQGTETLIGLAASTGLAVMI 120

Query: 121 VILSTGHISGAHLNPAITIAFAALKHFPWKHVPVYIGAQMVASLCASFALKWIFDPIMGG 180
           VI STGHISGAHLNPAITIAFAALKHFPWKHVPVYIGAQMVASLCASFALKWIFDPIMGG
Sbjct: 121 VISSTGHISGAHLNPAITIAFAALKHFPWKHVPVYIGAQMVASLCASFALKWIFDPIMGG 180

Query: 181 GVTVPSVGYEQAFALEFIISFNLMFVITAVATDTRAVGDLAGIAVGATVMLNVLIAGETT 240
           GVTVPSVGYEQAFALEFIISFNLMFVITAVATDTRAVGDLAGIAVGATVMLNVLIAGETT
Sbjct: 181 GVTVPSVGYEQAFALEFIISFNLMFVITAVATDTRAVGDLAGIAVGATVMLNVLIAGETT 240

Query: 241 GASMNPVRTLGPAIVTNNFKAIWIYLTAPILGTLCGAGIYTAVKLPDEHGDVHRKPSAER 300
           GASMNPVRTLGPAIVTNNFKAIW+YLTAPILGTLCGAGIYTAVKLPDEHGDVHRKPSAER
Sbjct: 241 GASMNPVRTLGPAIVTNNFKAIWVYLTAPILGTLCGAGIYTAVKLPDEHGDVHRKPSAER 300

Query: 301 SFRR 305
           SFRR
Sbjct: 301 SFRR 301

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BLAST of MS016234 vs. NCBI nr
Match: XP_008454166.1 (PREDICTED: aquaporin NIP6-1 [Cucumis melo] >TYK29615.1 aquaporin NIP6-1 [Cucumis melo var. makuwa])

HSP 1 Score: 488.4 bits (1256), Expect = 4.4e-134
Identity = 260/306 (84.97%), Postives = 274/306 (89.54%), Query Frame = 0


Query: 1   MDTDQEAPSTPVTPGTPGAPLFGGIRAEHHGNGNGKRSLLKSCISFNADD-WTNEERGLS 60
           MDT +EAPSTPVTPGTPGAPLFG ++   HG+ NGKRSLLKS I FN DD W  EE GLS
Sbjct: 1   MDT-EEAPSTPVTPGTPGAPLFGRVKENQHGSENGKRSLLKSWICFNVDDSWGIEEGGLS 60

Query: 61  SKV-SCSLSPPPISLARKVGAEFIATLMLIFAGTATAIVNQKTQGTETLIGLAASTGLAV 120
             V SCSL  PP+SLARKVGAEFI TL+LIFAGTATAIVNQKT+GTETLIGLAASTGLAV
Sbjct: 61  KIVSSCSLPYPPVSLARKVGAEFIGTLILIFAGTATAIVNQKTEGTETLIGLAASTGLAV 120

Query: 121 MIVILSTGHISGAHLNPAITIAFAALKHFPWKHVPVYIGAQMVASLCASFALKWIFDPIM 180
           MIVILSTGHISGAHLNPA+TIAFAALK FPWKHVP+YIGAQMVASLC+SFALKWIFDPIM
Sbjct: 121 MIVILSTGHISGAHLNPAVTIAFAALKQFPWKHVPLYIGAQMVASLCSSFALKWIFDPIM 180

Query: 181 GGGVTVPSVGYEQAFALEFIISFNLMFVITAVATDTRAVGDLAGIAVGATVMLNVLIAGE 240
           GGG TVPS GY QAFALEFIISFNLMFV+TAVATDTRAVG+LAGIAVGATVMLNVLIAG+
Sbjct: 181 GGGATVPSCGYAQAFALEFIISFNLMFVLTAVATDTRAVGELAGIAVGATVMLNVLIAGQ 240

Query: 241 TTGASMNPVRTLGPAIVTNNFKAIWIYLTAPILGTLCGAGIYTAVKLPDEHGDVHRKPSA 300
           TTGASMNPVRTLGPAI  NNFKAIWIYLTAPILGTLCGAGIYTAVKLPD+ GD  R PS 
Sbjct: 241 TTGASMNPVRTLGPAIAANNFKAIWIYLTAPILGTLCGAGIYTAVKLPDKDGD-SRLPST 300

Query: 301 ERSFRR 305
             SFRR
Sbjct: 301 AASFRR 304

[Close]

BLAST of MS016234 vs. NCBI nr
Match: XP_004152170.1 (aquaporin NIP6-1 [Cucumis sativus] >KGN52947.1 hypothetical protein Csa_015162 [Cucumis sativus])

HSP 1 Score: 488.0 bits (1255), Expect = 5.8e-134
Identity = 258/306 (84.31%), Postives = 274/306 (89.54%), Query Frame = 0


Query: 1   MDTDQEAPSTPVTPGTPGAPLFGGIRAEHHGNGNGKRSLLKSCISFNADD-WTNEERGLS 60
           MDT +EAPSTPVTPGTPGAPLFG ++   HG+ NGKRSLLKSCISFN DD W +EE GL+
Sbjct: 1   MDT-EEAPSTPVTPGTPGAPLFGRVKENQHGSENGKRSLLKSCISFNVDDNWGSEEGGLT 60

Query: 61  SKV-SCSLSPPPISLARKVGAEFIATLMLIFAGTATAIVNQKTQGTETLIGLAASTGLAV 120
             V SCSL   P+SLARKVGAEFI TL+LIFAGTATAIVNQKT GTETLIGLAASTGLAV
Sbjct: 61  KIVSSCSLPYSPVSLARKVGAEFIGTLILIFAGTATAIVNQKTGGTETLIGLAASTGLAV 120

Query: 121 MIVILSTGHISGAHLNPAITIAFAALKHFPWKHVPVYIGAQMVASLCASFALKWIFDPIM 180
           MIVILSTGHISGAHLNPA+TIAFAALK FPWKHVP+YIGAQMVASLC+SFALKW FDPIM
Sbjct: 121 MIVILSTGHISGAHLNPAVTIAFAALKQFPWKHVPLYIGAQMVASLCSSFALKWTFDPIM 180

Query: 181 GGGVTVPSVGYEQAFALEFIISFNLMFVITAVATDTRAVGDLAGIAVGATVMLNVLIAGE 240
           GGG T+PS GY QAFALEFIISFNLMFV+TAVATDTRAVG+LAGIAVGATVMLNVLIAG+
Sbjct: 181 GGGATIPSCGYAQAFALEFIISFNLMFVLTAVATDTRAVGELAGIAVGATVMLNVLIAGQ 240

Query: 241 TTGASMNPVRTLGPAIVTNNFKAIWIYLTAPILGTLCGAGIYTAVKLPDEHGDVHRKPSA 300
           TTGASMNPVRTLGPAI  NNFKAIWIYLTAPILGTLCGAGIYTAVKLPD+ GD  R PS 
Sbjct: 241 TTGASMNPVRTLGPAIAVNNFKAIWIYLTAPILGTLCGAGIYTAVKLPDKDGD-SRLPST 300

Query: 301 ERSFRR 305
             SFRR
Sbjct: 301 AASFRR 304

[Close]

BLAST of MS016234 vs. NCBI nr
Match: KAA0044488.1 (aquaporin NIP6-1 [Cucumis melo var. makuwa])

HSP 1 Score: 488.0 bits (1255), Expect = 5.8e-134
Identity = 259/306 (84.64%), Postives = 274/306 (89.54%), Query Frame = 0


Query: 1   MDTDQEAPSTPVTPGTPGAPLFGGIRAEHHGNGNGKRSLLKSCISFNADD-WTNEERGLS 60
           MDT +EAPSTPVTPGTPGAPLFG ++   HG+ NGKRSLLKS I FN DD W  EE GLS
Sbjct: 1   MDT-EEAPSTPVTPGTPGAPLFGRVKENQHGSENGKRSLLKSWICFNVDDSWGIEEGGLS 60

Query: 61  SKV-SCSLSPPPISLARKVGAEFIATLMLIFAGTATAIVNQKTQGTETLIGLAASTGLAV 120
             V SCSL  PP+SLARKVGAEFI TL+LIFAGTATAIVNQKT+GTETLIGLAASTGLAV
Sbjct: 61  KIVSSCSLPYPPVSLARKVGAEFIGTLILIFAGTATAIVNQKTEGTETLIGLAASTGLAV 120

Query: 121 MIVILSTGHISGAHLNPAITIAFAALKHFPWKHVPVYIGAQMVASLCASFALKWIFDPIM 180
           MIVILSTGHISGAHLNPA+TIAFAALK FPWKHVP+YIGAQMVASLC+SFALKWIFDPIM
Sbjct: 121 MIVILSTGHISGAHLNPAVTIAFAALKQFPWKHVPLYIGAQMVASLCSSFALKWIFDPIM 180

Query: 181 GGGVTVPSVGYEQAFALEFIISFNLMFVITAVATDTRAVGDLAGIAVGATVMLNVLIAGE 240
           GGG TVPS GY QAFALEFIISFNLMFV+TAVATDTRAVG+LAGIAVGATVMLN+LIAG+
Sbjct: 181 GGGATVPSCGYAQAFALEFIISFNLMFVLTAVATDTRAVGELAGIAVGATVMLNILIAGQ 240

Query: 241 TTGASMNPVRTLGPAIVTNNFKAIWIYLTAPILGTLCGAGIYTAVKLPDEHGDVHRKPSA 300
           TTGASMNPVRTLGPAI  NNFKAIWIYLTAPILGTLCGAGIYTAVKLPD+ GD  R PS 
Sbjct: 241 TTGASMNPVRTLGPAIAANNFKAIWIYLTAPILGTLCGAGIYTAVKLPDKDGD-SRLPST 300

Query: 301 ERSFRR 305
             SFRR
Sbjct: 301 AASFRR 304

[Close]

BLAST of MS016234 vs. NCBI nr
Match: XP_038876197.1 (aquaporin NIP6-1 [Benincasa hispida])

HSP 1 Score: 485.7 bits (1249), Expect = 2.9e-133
Identity = 257/306 (83.99%), Postives = 272/306 (88.89%), Query Frame = 0


Query: 1   MDTDQEAPSTPVTPGTPGAPLFGGIRAEHHGNGNGKRSLLKSCISFNADD-WTNEERGLS 60
           MDT +E PSTPVTPGTPGAPLFG ++   HG+ NGKRSLLKSCISFN DD W  EE GLS
Sbjct: 1   MDT-EETPSTPVTPGTPGAPLFGRVKENRHGSENGKRSLLKSCISFNVDDNWGTEEGGLS 60

Query: 61  SKV-SCSLSPPPISLARKVGAEFIATLMLIFAGTATAIVNQKTQGTETLIGLAASTGLAV 120
             V SCSL  PP+SLARKVGAEFI TL+LIFAGTATAIVNQKT+GTE LIGLAASTGLAV
Sbjct: 61  KLVSSCSLPYPPVSLARKVGAEFIGTLILIFAGTATAIVNQKTEGTEMLIGLAASTGLAV 120

Query: 121 MIVILSTGHISGAHLNPAITIAFAALKHFPWKHVPVYIGAQMVASLCASFALKWIFDPIM 180
           MIVILSTGHISGAHLNPAITIAFAALK FPWK+VPVYIGAQMVASLC+SFALKW+FDPIM
Sbjct: 121 MIVILSTGHISGAHLNPAITIAFAALKQFPWKYVPVYIGAQMVASLCSSFALKWMFDPIM 180

Query: 181 GGGVTVPSVGYEQAFALEFIISFNLMFVITAVATDTRAVGDLAGIAVGATVMLNVLIAGE 240
           GGG TVPS GY QAFALEFIISFNLMFV+TAVATDTRAVG+LAGIAVGATVMLNVLIAG+
Sbjct: 181 GGGATVPSCGYAQAFALEFIISFNLMFVLTAVATDTRAVGELAGIAVGATVMLNVLIAGQ 240

Query: 241 TTGASMNPVRTLGPAIVTNNFKAIWIYLTAPILGTLCGAGIYTAVKLPDEHGDVHRKPSA 300
            TGASMNPVRTLGPAI  NNFKA+WIYLTAPILG LCGAGIYTAVKLPD+ GD  R PS 
Sbjct: 241 ATGASMNPVRTLGPAIAANNFKAVWIYLTAPILGALCGAGIYTAVKLPDKDGD-SRLPST 300

Query: 301 ERSFRR 305
             SFRR
Sbjct: 301 AGSFRR 304

[Close]

BLAST of MS016234 vs. ExPASy Swiss-Prot
Match: Q9SAI4 (Aquaporin NIP6-1 OS=Arabidopsis thaliana OX=3702 GN=NIP6-1 PE=1 SV=1)

HSP 1 Score: 409.8 bits (1052), Expect = 2.6e-113
Identity = 220/307 (71.66%), Postives = 248/307 (80.78%), Query Frame = 0


Query: 5   QEAPSTP----VTPGTPGAPLFGGIRAEHHGNGNGK---RSLLKSCISFNADDWTNEERG 64
           +E PSTP     TPGTPGAPLFGG   + +G+ NG+   +SLLKSC  F+ D+    E G
Sbjct: 4   EEIPSTPSTPATTPGTPGAPLFGGFEGKRNGH-NGRYTPKSLLKSCKCFSVDNEWALEDG 63

Query: 65  LSSKVSCSLSPPPISLARKVGAEFIATLMLIFAGTATAIVNQKTQGTETLIGLAASTGLA 124
               V+CSL PP +SL RK+GAEF+ TL+LIFAGTATAIVNQKT G ETLIG AAS GLA
Sbjct: 64  RLPPVTCSLPPPNVSLYRKLGAEFVGTLILIFAGTATAIVNQKTDGAETLIGCAASAGLA 123

Query: 125 VMIVILSTGHISGAHLNPAITIAFAALKHFPWKHVPVYIGAQMVASLCASFALKWIFDPI 184
           VMIVILSTGHISGAHLNPA+TIAFAALKHFPWKHVPVYIGAQ++AS+ A+FALK +F+P 
Sbjct: 124 VMIVILSTGHISGAHLNPAVTIAFAALKHFPWKHVPVYIGAQVMASVSAAFALKAVFEPT 183

Query: 185 MGGGVTVPSVGYEQAFALEFIISFNLMFVITAVATDTRAVGDLAGIAVGATVMLNVLIAG 244
           M GGVTVP+VG  QAFALEFIISFNLMFV+TAVATDTRAVG+LAGIAVGATVMLN+LIAG
Sbjct: 184 MSGGVTVPTVGLSQAFALEFIISFNLMFVVTAVATDTRAVGELAGIAVGATVMLNILIAG 243

Query: 245 ETTGASMNPVRTLGPAIVTNNFKAIWIYLTAPILGTLCGAGIYTAVKLPDEHGDVHRKPS 304
             T ASMNPVRTLGPAI  NN++AIW+YLTAPILG L GAG YT VKLP+E       P 
Sbjct: 244 PATSASMNPVRTLGPAIAANNYRAIWVYLTAPILGALIGAGTYTIVKLPEE----DEAPK 303

[Close]

BLAST of MS016234 vs. ExPASy Swiss-Prot
Match: Q9SV84 (Probable aquaporin NIP5-1 OS=Arabidopsis thaliana OX=3702 GN=NIP5-1 PE=2 SV=1)

HSP 1 Score: 338.2 bits (866), Expect = 9.7e-92
Identity = 179/298 (60.07%), Postives = 212/298 (71.14%), Query Frame = 0


Query: 7   APSTPVTPGTPGAPLFGGIRAEHHGNGNGKRSLLKSCISFNADDWTNEERGLSSKVSCSL 66
           AP TP TPGTPG PL  G+R +     + K +    C+      W     G         
Sbjct: 15  APPTPGTPGTPGGPLITGMRVDSMSFDHRKPTPRCKCLPVMGSTW-----GQHDTCFTDF 74

Query: 67  SPPPISLARKVGAEFIATLMLIFAGTATAIVNQKTQGTETLIGLAASTGLAVMIVILSTG 126
             P +SL RK+GAEF+ T +LIF  TA  IVNQK  G ETLIG AA  GLAVMI+ILSTG
Sbjct: 75  PSPDVSLTRKLGAEFVGTFILIFTATAGPIVNQKYDGAETLIGNAACAGLAVMIIILSTG 134

Query: 127 HISGAHLNPAITIAFAALKHFPWKHVPVYIGAQMVASLCASFALKWIFDPIMGGGVTVPS 186
           HISGAHLNP++TIAFAAL+HFPW HVP YI AQ+ AS+CASFALK +F P M GGVT+PS
Sbjct: 135 HISGAHLNPSLTIAFAALRHFPWAHVPAYIAAQVSASICASFALKGVFHPFMSGGVTIPS 194

Query: 187 VGYEQAFALEFIISFNLMFVITAVATDTRAVGDLAGIAVGATVMLNVLIAGETTGASMNP 246
           V   QAFALEFII+F L+FV+TAVATDTRAVG+LAGIAVGATVMLN+L+AG +TG SMNP
Sbjct: 195 VSLGQAFALEFIITFILLFVVTAVATDTRAVGELAGIAVGATVMLNILVAGPSTGGSMNP 254

Query: 247 VRTLGPAIVTNNFKAIWIYLTAPILGTLCGAGIYTAVKLPDEHGDVHRKPSAERSFRR 305
           VRTLGPA+ + N++++W+YL AP LG + GA +YT VKL D   D    P   RSFRR
Sbjct: 255 VRTLGPAVASGNYRSLWVYLVAPTLGAISGAAVYTGVKLNDSVTD---PPRPVRSFRR 304

[Close]

BLAST of MS016234 vs. ExPASy Swiss-Prot
Match: Q0IWF3 (Aquaporin NIP3-1 OS=Oryza sativa subsp. japonica OX=39947 GN=NIP3-1 PE=2 SV=2)

HSP 1 Score: 310.1 bits (793), Expect = 2.8e-83
Identity = 169/306 (55.23%), Postives = 206/306 (67.32%), Query Frame = 0


Query: 1   MDTDQEAPSTPVTPGTPGAPLFGGIRAEHHGNGNGKRSLLKSCISFNADDWTNEERGLSS 60
           M +     S P TPGTP APLF G R +            K   +  A+ W     G   
Sbjct: 14  MSSPVNGASAPATPGTP-APLFAGPRVDSLSYERKSMPRCKCLPAAVAEAWAPSAHGCVV 73

Query: 61  KVSCSLSPPPISLARKVGAEFIATLMLIFAGTATAIVNQKTQGTETLIGLAASTGLAVMI 120
           ++      P +SL RK+GAEF+ T +LIF  TA  IVNQK  G  +  G AA  GLAV  
Sbjct: 74  EIPA----PDVSLTRKLGAEFVGTFILIFFATAAPIVNQKYGGAISPFGNAACAGLAVTT 133

Query: 121 VILSTGHISGAHLNPAITIAFAALKHFPWKHVPVYIGAQMVASLCASFALKWIFDPIMGG 180
           +ILSTGHISGAHLNP++TIAFAAL+HFPW  VP Y+  Q++ S+CA FALK +F P + G
Sbjct: 134 IILSTGHISGAHLNPSLTIAFAALRHFPWLQVPAYVAVQVLGSICAGFALKGVFHPFLSG 193

Query: 181 GVTV--PSVGYEQAFALEFIISFNLMFVITAVATDTRAVGDLAGIAVGATVMLNVLIAGE 240
           GVTV  P++   QAF  EFII+FNL+FV+TAVATDTRAVG+LAGIAVGA V LN+LIAG 
Sbjct: 194 GVTVPDPTISTAQAFFTEFIITFNLLFVVTAVATDTRAVGELAGIAVGAAVTLNILIAGP 253

Query: 241 TTGASMNPVRTLGPAIVTNNFKAIWIYLTAPILGTLCGAGIYTAVKLPDEHGDVHRKPSA 300
           TTG SMNPVRTLGPA+   N++ +WIYL AP LG + GAG+YTAVKL DE+G+    P  
Sbjct: 254 TTGGSMNPVRTLGPAVAAGNYRQLWIYLIAPTLGAVAGAGVYTAVKLRDENGET---PRP 311

Query: 301 ERSFRR 305
           +RSFRR
Sbjct: 314 QRSFRR 311

[Close]

BLAST of MS016234 vs. ExPASy Swiss-Prot
Match: Q9ATN1 (Aquaporin NIP3-1 OS=Zea mays OX=4577 GN=NIP3-1 PE=2 SV=1)

HSP 1 Score: 300.4 bits (768), Expect = 2.2e-80
Identity = 172/302 (56.95%), Postives = 207/302 (68.54%), Query Frame = 0


Query: 9   STPVTPGTPGAPLF--GGIRAEHHGNGNGKRSLLKSCISFNA-DDWTNEERGLSSKVS-C 68
           S P TPGTP APLF  GG R +            K C+   A + W     G+++     
Sbjct: 11  SAPATPGTP-APLFSSGGPRVDSLSYERKSMPRCK-CLPLPAVEGW-----GVATHTCVV 70

Query: 69  SLSPPPISLARKVGAEFIATLMLIFAGTATAIVNQKTQGTETLIGLAASTGLAVMIVILS 128
            +  P +SL RK+GAEF+ T +LIF  TA  IVNQK  G  +  G AA  GLAV  VILS
Sbjct: 71  EIPAPDVSLTRKLGAEFVGTFILIFFATAAPIVNQKYGGAISPFGNAACAGLAVATVILS 130

Query: 129 TGHISGAHLNPAITIAFAALKHFPWKHVPVYIGAQMVASLCASFALKWIFDPIMGGGVTV 188
           TGHISGAHLNP++TIAFAAL+HFPW  VP Y+  Q +AS+CA+FALK +F P + GGVTV
Sbjct: 131 TGHISGAHLNPSLTIAFAALRHFPWLQVPAYVAVQALASVCAAFALKGVFHPFLSGGVTV 190

Query: 189 P--SVGYEQAFALEFIISFNLMFVITAVATDTRAVGDLAGIAVGATVMLNVLIAGETTGA 248
           P  +V   QAF  EFIISFNL+FV+TAVATDTRAVG+LAGIAVGA V LN+L+AG TTG 
Sbjct: 191 PDATVSTAQAFFTEFIISFNLLFVVTAVATDTRAVGELAGIAVGAAVTLNILVAGPTTGG 250

Query: 249 SMNPVRTLGPAIVTNNFKAIWIYLTAPILGTLCGAGIYTAVKLPDEHGDVHRKPSAERSF 305
           SMNPVRTLGPA+   N++ +WIYL AP LG L GA +Y AVKL DE+G+    P  +RSF
Sbjct: 251 SMNPVRTLGPAVAAGNYRQLWIYLLAPTLGALAGASVYKAVKLRDENGET---PRTQRSF 302

[Close]

BLAST of MS016234 vs. ExPASy Swiss-Prot
Match: Q7EYH7 (Aquaporin NIP3-2 OS=Oryza sativa subsp. japonica OX=39947 GN=NIP3-2 PE=2 SV=1)

HSP 1 Score: 225.7 bits (574), Expect = 7.0e-58
Identity = 114/217 (52.53%), Postives = 154/217 (70.97%), Query Frame = 0


Query: 69  PPISLARKVGAEFIATLMLIFAGTATAIVNQKTQGTETLIGLAASTGLAVMIVILSTGHI 128
           PP+ L +KVGAEF  T  LIF   +T I++++ +G E+L+G+A S GLAV +++LS  HI
Sbjct: 89  PPVPLVKKVGAEFFGTFTLIFTVLSTIIMDEQHKGVESLLGIATSAGLAVTVLVLSLIHI 148

Query: 129 SGAHLNPAITIAFAALKHFPWKHVPVYIGAQMVASLCASFALKWIFDPIMGGGVTVPSVG 188
           SG HLNPA++IA     H P  H+  YI AQ++ S+ ASFA+K ++ P+  G VTVP VG
Sbjct: 149 SGCHLNPAVSIAMTVFGHLPPAHLLPYIAAQILGSITASFAVKGMYHPVNPGIVTVPKVG 208

Query: 189 YEQAFALEFIISFNLMFVITAVATDTRAVGDLAGIAVGATVMLNVLIAGETTGASMNPVR 248
             +AF LEF+ +F L+F+ITA+ATD  AV +L  +AVGAT+M+N L+AG +TGASMNP R
Sbjct: 209 TVEAFFLEFVTTFVLLFIITALATDPNAVKELIAVAVGATIMMNALVAGPSTGASMNPAR 268

Query: 249 TLGPAIVTNNFKAIWIYLTAPILGTLCGAGIYTAVKL 286
           TLGPAI T  +  IW+YL A  LG + G G Y A+KL
Sbjct: 269 TLGPAIATGRYTQIWVYLVATPLGAVAGEGFYFAIKL 305

[Close]

BLAST of MS016234 vs. ExPASy TrEMBL
Match: A0A6J1DHD3 (aquaporin NIP6-1 OS=Momordica charantia OX=3673 GN=LOC111020915 PE=3 SV=1)

HSP 1 Score: 576.6 bits (1485), Expect = 6.0e-161
Identity = 296/304 (97.37%), Postives = 299/304 (98.36%), Query Frame = 0


Query: 1   MDTDQEAPSTPVTPGTPGAPLFGGIRAEHHGNGNGKRSLLKSCISFNADDWTNEERGLSS 60
           MDTDQEAPS   TPGTPGAPLF GIRAEHHGNGNGKRSLLK+CISFNADDWTNEERGLSS
Sbjct: 1   MDTDQEAPS---TPGTPGAPLFEGIRAEHHGNGNGKRSLLKNCISFNADDWTNEERGLSS 60

Query: 61  KVSCSLSPPPISLARKVGAEFIATLMLIFAGTATAIVNQKTQGTETLIGLAASTGLAVMI 120
           KVSCSLSPPPISLARKVGAEFI+TLMLIFAGTATAIVNQKTQGTETLIGLAASTGLAVMI
Sbjct: 61  KVSCSLSPPPISLARKVGAEFISTLMLIFAGTATAIVNQKTQGTETLIGLAASTGLAVMI 120

Query: 121 VILSTGHISGAHLNPAITIAFAALKHFPWKHVPVYIGAQMVASLCASFALKWIFDPIMGG 180
           VI STGHISGAHLNPAITIAFAALKHFPWKHVPVYIGAQMVASLCASFALKWIFDPIMGG
Sbjct: 121 VISSTGHISGAHLNPAITIAFAALKHFPWKHVPVYIGAQMVASLCASFALKWIFDPIMGG 180

Query: 181 GVTVPSVGYEQAFALEFIISFNLMFVITAVATDTRAVGDLAGIAVGATVMLNVLIAGETT 240
           GVTVPSVGYEQAFALEFIISFNLMFVITAVATDTRAVGDLAGIAVGATVMLNVLIAGETT
Sbjct: 181 GVTVPSVGYEQAFALEFIISFNLMFVITAVATDTRAVGDLAGIAVGATVMLNVLIAGETT 240

Query: 241 GASMNPVRTLGPAIVTNNFKAIWIYLTAPILGTLCGAGIYTAVKLPDEHGDVHRKPSAER 300
           GASMNPVRTLGPAIVTNNFKAIW+YLTAPILGTLCGAGIYTAVKLPDEHGDVHRKPSAER
Sbjct: 241 GASMNPVRTLGPAIVTNNFKAIWVYLTAPILGTLCGAGIYTAVKLPDEHGDVHRKPSAER 300

Query: 301 SFRR 305
           SFRR
Sbjct: 301 SFRR 301

[Close]

BLAST of MS016234 vs. ExPASy TrEMBL
Match: A0A5D3E2S7 (Aquaporin NIP6-1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G002260 PE=3 SV=1)

HSP 1 Score: 488.4 bits (1256), Expect = 2.1e-134
Identity = 260/306 (84.97%), Postives = 274/306 (89.54%), Query Frame = 0


Query: 1   MDTDQEAPSTPVTPGTPGAPLFGGIRAEHHGNGNGKRSLLKSCISFNADD-WTNEERGLS 60
           MDT +EAPSTPVTPGTPGAPLFG ++   HG+ NGKRSLLKS I FN DD W  EE GLS
Sbjct: 1   MDT-EEAPSTPVTPGTPGAPLFGRVKENQHGSENGKRSLLKSWICFNVDDSWGIEEGGLS 60

Query: 61  SKV-SCSLSPPPISLARKVGAEFIATLMLIFAGTATAIVNQKTQGTETLIGLAASTGLAV 120
             V SCSL  PP+SLARKVGAEFI TL+LIFAGTATAIVNQKT+GTETLIGLAASTGLAV
Sbjct: 61  KIVSSCSLPYPPVSLARKVGAEFIGTLILIFAGTATAIVNQKTEGTETLIGLAASTGLAV 120

Query: 121 MIVILSTGHISGAHLNPAITIAFAALKHFPWKHVPVYIGAQMVASLCASFALKWIFDPIM 180
           MIVILSTGHISGAHLNPA+TIAFAALK FPWKHVP+YIGAQMVASLC+SFALKWIFDPIM
Sbjct: 121 MIVILSTGHISGAHLNPAVTIAFAALKQFPWKHVPLYIGAQMVASLCSSFALKWIFDPIM 180

Query: 181 GGGVTVPSVGYEQAFALEFIISFNLMFVITAVATDTRAVGDLAGIAVGATVMLNVLIAGE 240
           GGG TVPS GY QAFALEFIISFNLMFV+TAVATDTRAVG+LAGIAVGATVMLNVLIAG+
Sbjct: 181 GGGATVPSCGYAQAFALEFIISFNLMFVLTAVATDTRAVGELAGIAVGATVMLNVLIAGQ 240

Query: 241 TTGASMNPVRTLGPAIVTNNFKAIWIYLTAPILGTLCGAGIYTAVKLPDEHGDVHRKPSA 300
           TTGASMNPVRTLGPAI  NNFKAIWIYLTAPILGTLCGAGIYTAVKLPD+ GD  R PS 
Sbjct: 241 TTGASMNPVRTLGPAIAANNFKAIWIYLTAPILGTLCGAGIYTAVKLPDKDGD-SRLPST 300

Query: 301 ERSFRR 305
             SFRR
Sbjct: 301 AASFRR 304

[Close]

BLAST of MS016234 vs. ExPASy TrEMBL
Match: A0A1S3BY36 (aquaporin NIP6-1 OS=Cucumis melo OX=3656 GN=LOC103494651 PE=3 SV=1)

HSP 1 Score: 488.4 bits (1256), Expect = 2.1e-134
Identity = 260/306 (84.97%), Postives = 274/306 (89.54%), Query Frame = 0


Query: 1   MDTDQEAPSTPVTPGTPGAPLFGGIRAEHHGNGNGKRSLLKSCISFNADD-WTNEERGLS 60
           MDT +EAPSTPVTPGTPGAPLFG ++   HG+ NGKRSLLKS I FN DD W  EE GLS
Sbjct: 1   MDT-EEAPSTPVTPGTPGAPLFGRVKENQHGSENGKRSLLKSWICFNVDDSWGIEEGGLS 60

Query: 61  SKV-SCSLSPPPISLARKVGAEFIATLMLIFAGTATAIVNQKTQGTETLIGLAASTGLAV 120
             V SCSL  PP+SLARKVGAEFI TL+LIFAGTATAIVNQKT+GTETLIGLAASTGLAV
Sbjct: 61  KIVSSCSLPYPPVSLARKVGAEFIGTLILIFAGTATAIVNQKTEGTETLIGLAASTGLAV 120

Query: 121 MIVILSTGHISGAHLNPAITIAFAALKHFPWKHVPVYIGAQMVASLCASFALKWIFDPIM 180
           MIVILSTGHISGAHLNPA+TIAFAALK FPWKHVP+YIGAQMVASLC+SFALKWIFDPIM
Sbjct: 121 MIVILSTGHISGAHLNPAVTIAFAALKQFPWKHVPLYIGAQMVASLCSSFALKWIFDPIM 180

Query: 181 GGGVTVPSVGYEQAFALEFIISFNLMFVITAVATDTRAVGDLAGIAVGATVMLNVLIAGE 240
           GGG TVPS GY QAFALEFIISFNLMFV+TAVATDTRAVG+LAGIAVGATVMLNVLIAG+
Sbjct: 181 GGGATVPSCGYAQAFALEFIISFNLMFVLTAVATDTRAVGELAGIAVGATVMLNVLIAGQ 240

Query: 241 TTGASMNPVRTLGPAIVTNNFKAIWIYLTAPILGTLCGAGIYTAVKLPDEHGDVHRKPSA 300
           TTGASMNPVRTLGPAI  NNFKAIWIYLTAPILGTLCGAGIYTAVKLPD+ GD  R PS 
Sbjct: 241 TTGASMNPVRTLGPAIAANNFKAIWIYLTAPILGTLCGAGIYTAVKLPDKDGD-SRLPST 300

Query: 301 ERSFRR 305
             SFRR
Sbjct: 301 AASFRR 304

[Close]

BLAST of MS016234 vs. ExPASy TrEMBL
Match: A0A5A7TNN6 (Aquaporin NIP6-1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G002250 PE=3 SV=1)

HSP 1 Score: 488.0 bits (1255), Expect = 2.8e-134
Identity = 259/306 (84.64%), Postives = 274/306 (89.54%), Query Frame = 0


Query: 1   MDTDQEAPSTPVTPGTPGAPLFGGIRAEHHGNGNGKRSLLKSCISFNADD-WTNEERGLS 60
           MDT +EAPSTPVTPGTPGAPLFG ++   HG+ NGKRSLLKS I FN DD W  EE GLS
Sbjct: 1   MDT-EEAPSTPVTPGTPGAPLFGRVKENQHGSENGKRSLLKSWICFNVDDSWGIEEGGLS 60

Query: 61  SKV-SCSLSPPPISLARKVGAEFIATLMLIFAGTATAIVNQKTQGTETLIGLAASTGLAV 120
             V SCSL  PP+SLARKVGAEFI TL+LIFAGTATAIVNQKT+GTETLIGLAASTGLAV
Sbjct: 61  KIVSSCSLPYPPVSLARKVGAEFIGTLILIFAGTATAIVNQKTEGTETLIGLAASTGLAV 120

Query: 121 MIVILSTGHISGAHLNPAITIAFAALKHFPWKHVPVYIGAQMVASLCASFALKWIFDPIM 180
           MIVILSTGHISGAHLNPA+TIAFAALK FPWKHVP+YIGAQMVASLC+SFALKWIFDPIM
Sbjct: 121 MIVILSTGHISGAHLNPAVTIAFAALKQFPWKHVPLYIGAQMVASLCSSFALKWIFDPIM 180

Query: 181 GGGVTVPSVGYEQAFALEFIISFNLMFVITAVATDTRAVGDLAGIAVGATVMLNVLIAGE 240
           GGG TVPS GY QAFALEFIISFNLMFV+TAVATDTRAVG+LAGIAVGATVMLN+LIAG+
Sbjct: 181 GGGATVPSCGYAQAFALEFIISFNLMFVLTAVATDTRAVGELAGIAVGATVMLNILIAGQ 240

Query: 241 TTGASMNPVRTLGPAIVTNNFKAIWIYLTAPILGTLCGAGIYTAVKLPDEHGDVHRKPSA 300
           TTGASMNPVRTLGPAI  NNFKAIWIYLTAPILGTLCGAGIYTAVKLPD+ GD  R PS 
Sbjct: 241 TTGASMNPVRTLGPAIAANNFKAIWIYLTAPILGTLCGAGIYTAVKLPDKDGD-SRLPST 300

Query: 301 ERSFRR 305
             SFRR
Sbjct: 301 AASFRR 304

[Close]

BLAST of MS016234 vs. ExPASy TrEMBL
Match: A0A0A0KX08 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G007030 PE=3 SV=1)

HSP 1 Score: 488.0 bits (1255), Expect = 2.8e-134
Identity = 258/306 (84.31%), Postives = 274/306 (89.54%), Query Frame = 0


Query: 1   MDTDQEAPSTPVTPGTPGAPLFGGIRAEHHGNGNGKRSLLKSCISFNADD-WTNEERGLS 60
           MDT +EAPSTPVTPGTPGAPLFG ++   HG+ NGKRSLLKSCISFN DD W +EE GL+
Sbjct: 1   MDT-EEAPSTPVTPGTPGAPLFGRVKENQHGSENGKRSLLKSCISFNVDDNWGSEEGGLT 60

Query: 61  SKV-SCSLSPPPISLARKVGAEFIATLMLIFAGTATAIVNQKTQGTETLIGLAASTGLAV 120
             V SCSL   P+SLARKVGAEFI TL+LIFAGTATAIVNQKT GTETLIGLAASTGLAV
Sbjct: 61  KIVSSCSLPYSPVSLARKVGAEFIGTLILIFAGTATAIVNQKTGGTETLIGLAASTGLAV 120

Query: 121 MIVILSTGHISGAHLNPAITIAFAALKHFPWKHVPVYIGAQMVASLCASFALKWIFDPIM 180
           MIVILSTGHISGAHLNPA+TIAFAALK FPWKHVP+YIGAQMVASLC+SFALKW FDPIM
Sbjct: 121 MIVILSTGHISGAHLNPAVTIAFAALKQFPWKHVPLYIGAQMVASLCSSFALKWTFDPIM 180

Query: 181 GGGVTVPSVGYEQAFALEFIISFNLMFVITAVATDTRAVGDLAGIAVGATVMLNVLIAGE 240
           GGG T+PS GY QAFALEFIISFNLMFV+TAVATDTRAVG+LAGIAVGATVMLNVLIAG+
Sbjct: 181 GGGATIPSCGYAQAFALEFIISFNLMFVLTAVATDTRAVGELAGIAVGATVMLNVLIAGQ 240

Query: 241 TTGASMNPVRTLGPAIVTNNFKAIWIYLTAPILGTLCGAGIYTAVKLPDEHGDVHRKPSA 300
           TTGASMNPVRTLGPAI  NNFKAIWIYLTAPILGTLCGAGIYTAVKLPD+ GD  R PS 
Sbjct: 241 TTGASMNPVRTLGPAIAVNNFKAIWIYLTAPILGTLCGAGIYTAVKLPDKDGD-SRLPST 300

Query: 301 ERSFRR 305
             SFRR
Sbjct: 301 AASFRR 304

[Close]

BLAST of MS016234 vs. TAIR 10
Match: AT1G80760.1 (NOD26-like intrinsic protein 6;1 )

HSP 1 Score: 409.8 bits (1052), Expect = 1.9e-114
Identity = 220/307 (71.66%), Postives = 248/307 (80.78%), Query Frame = 0


Query: 5   QEAPSTP----VTPGTPGAPLFGGIRAEHHGNGNGK---RSLLKSCISFNADDWTNEERG 64
           +E PSTP     TPGTPGAPLFGG   + +G+ NG+   +SLLKSC  F+ D+    E G
Sbjct: 4   EEIPSTPSTPATTPGTPGAPLFGGFEGKRNGH-NGRYTPKSLLKSCKCFSVDNEWALEDG 63

Query: 65  LSSKVSCSLSPPPISLARKVGAEFIATLMLIFAGTATAIVNQKTQGTETLIGLAASTGLA 124
               V+CSL PP +SL RK+GAEF+ TL+LIFAGTATAIVNQKT G ETLIG AAS GLA
Sbjct: 64  RLPPVTCSLPPPNVSLYRKLGAEFVGTLILIFAGTATAIVNQKTDGAETLIGCAASAGLA 123

Query: 125 VMIVILSTGHISGAHLNPAITIAFAALKHFPWKHVPVYIGAQMVASLCASFALKWIFDPI 184
           VMIVILSTGHISGAHLNPA+TIAFAALKHFPWKHVPVYIGAQ++AS+ A+FALK +F+P 
Sbjct: 124 VMIVILSTGHISGAHLNPAVTIAFAALKHFPWKHVPVYIGAQVMASVSAAFALKAVFEPT 183

Query: 185 MGGGVTVPSVGYEQAFALEFIISFNLMFVITAVATDTRAVGDLAGIAVGATVMLNVLIAG 244
           M GGVTVP+VG  QAFALEFIISFNLMFV+TAVATDTRAVG+LAGIAVGATVMLN+LIAG
Sbjct: 184 MSGGVTVPTVGLSQAFALEFIISFNLMFVVTAVATDTRAVGELAGIAVGATVMLNILIAG 243

Query: 245 ETTGASMNPVRTLGPAIVTNNFKAIWIYLTAPILGTLCGAGIYTAVKLPDEHGDVHRKPS 304
             T ASMNPVRTLGPAI  NN++AIW+YLTAPILG L GAG YT VKLP+E       P 
Sbjct: 244 PATSASMNPVRTLGPAIAANNYRAIWVYLTAPILGALIGAGTYTIVKLPEE----DEAPK 303

[Close]

BLAST of MS016234 vs. TAIR 10
Match: AT4G10380.1 (NOD26-like intrinsic protein 5;1 )

HSP 1 Score: 338.2 bits (866), Expect = 6.9e-93
Identity = 179/298 (60.07%), Postives = 212/298 (71.14%), Query Frame = 0


Query: 7   APSTPVTPGTPGAPLFGGIRAEHHGNGNGKRSLLKSCISFNADDWTNEERGLSSKVSCSL 66
           AP TP TPGTPG PL  G+R +     + K +    C+      W     G         
Sbjct: 15  APPTPGTPGTPGGPLITGMRVDSMSFDHRKPTPRCKCLPVMGSTW-----GQHDTCFTDF 74

Query: 67  SPPPISLARKVGAEFIATLMLIFAGTATAIVNQKTQGTETLIGLAASTGLAVMIVILSTG 126
             P +SL RK+GAEF+ T +LIF  TA  IVNQK  G ETLIG AA  GLAVMI+ILSTG
Sbjct: 75  PSPDVSLTRKLGAEFVGTFILIFTATAGPIVNQKYDGAETLIGNAACAGLAVMIIILSTG 134

Query: 127 HISGAHLNPAITIAFAALKHFPWKHVPVYIGAQMVASLCASFALKWIFDPIMGGGVTVPS 186
           HISGAHLNP++TIAFAAL+HFPW HVP YI AQ+ AS+CASFALK +F P M GGVT+PS
Sbjct: 135 HISGAHLNPSLTIAFAALRHFPWAHVPAYIAAQVSASICASFALKGVFHPFMSGGVTIPS 194

Query: 187 VGYEQAFALEFIISFNLMFVITAVATDTRAVGDLAGIAVGATVMLNVLIAGETTGASMNP 246
           V   QAFALEFII+F L+FV+TAVATDTRAVG+LAGIAVGATVMLN+L+AG +TG SMNP
Sbjct: 195 VSLGQAFALEFIITFILLFVVTAVATDTRAVGELAGIAVGATVMLNILVAGPSTGGSMNP 254

Query: 247 VRTLGPAIVTNNFKAIWIYLTAPILGTLCGAGIYTAVKLPDEHGDVHRKPSAERSFRR 305
           VRTLGPA+ + N++++W+YL AP LG + GA +YT VKL D   D    P   RSFRR
Sbjct: 255 VRTLGPAVASGNYRSLWVYLVAPTLGAISGAAVYTGVKLNDSVTD---PPRPVRSFRR 304

[Close]

BLAST of MS016234 vs. TAIR 10
Match: AT5G37820.1 (NOD26-like intrinsic protein 4;2 )

HSP 1 Score: 208.8 bits (530), Expect = 6.3e-54
Identity = 114/237 (48.10%), Postives = 151/237 (63.71%), Query Frame = 0


Query: 54  EERGLSSKVSCSLSPPPISLARKVGAEFIATLMLIFAGTATAIVNQKTQGTETLIGLAAS 113
           + +G      CS SP  + L +K+ AE I T  +IF+G    +VN    GT T  G+  +
Sbjct: 23  DSQGGMETAICS-SPSIVCLTQKLIAEMIGTYFIIFSGCGVVVVNVLYGGTITFPGICVT 82

Query: 114 TGLAVMIVILSTGHISGAHLNPAITIAFAALKHFPWKHVPVYIGAQMVASLCASFALKWI 173
            GL VM++I STGHISGAH NPA+T+ FA  + FPW  VP+YIGAQ+  SL AS  L+ +
Sbjct: 83  WGLIVMVMIYSTGHISGAHFNPAVTVTFAVFRRFPWYQVPLYIGAQLTGSLLASLTLRLM 142

Query: 174 FD--PIMGGGVTVPSVGYEQAFALEFIISFNLMFVITAVATDTRAVGDLAGIAVGATVML 233
           F+  P    G T P+    QA   E IISF LMFVI+ VATD+RA G+LAGIAVG T++L
Sbjct: 143 FNVTPKAFFG-TTPTDSSGQALVAEIIISFLLMFVISGVATDSRATGELAGIAVGMTIIL 202

Query: 234 NVLIAGETTGASMNPVRTLGPAIVTNNFKAIWIYLTAPILGTLCGAGIYTAVKLPDE 289
           NV +AG  +GASMNP R+LGPAIV   +K IW+Y+  P +G   G  +Y  ++  D+
Sbjct: 203 NVFVAGPISGASMNPARSLGPAIVMGRYKGIWVYIVGPFVGIFAGGFVYNFMRFTDK 257

[Close]

BLAST of MS016234 vs. TAIR 10
Match: AT5G37810.1 (NOD26-like intrinsic protein 4;1 )

HSP 1 Score: 208.0 bits (528), Expect = 1.1e-53
Identity = 109/226 (48.23%), Postives = 146/226 (64.60%), Query Frame = 0


Query: 67  SPPPISLARKVGAEFIATLMLIFAGTATAIVNQKTQGTETLIGLAASTGLAVMIVILSTG 126
           SP  + L +K+ AE I T  ++F+G    +VN    GT T  G+  + GL VM++I STG
Sbjct: 35  SPSIVCLTQKLIAEMIGTYFIVFSGCGVVVVNVLYGGTITFPGICVTWGLIVMVMIYSTG 94

Query: 127 HISGAHLNPAITIAFAALKHFPWKHVPVYIGAQMVASLCASFALKWIF----DPIMGGGV 186
           HISGAH NPA+T+ FA  + FPW  VP+YIGAQ   SL AS  L+ +F    +   G   
Sbjct: 95  HISGAHFNPAVTVTFAIFRRFPWHQVPLYIGAQFAGSLLASLTLRLMFKVTPEAFFG--- 154

Query: 187 TVPSVGYEQAFALEFIISFNLMFVITAVATDTRAVGDLAGIAVGATVMLNVLIAGETTGA 246
           T P+    +A   E IISF LMFVI+ VATD RAVG+LAGIAVG T+M+NV +AG  +GA
Sbjct: 155 TTPADSPARALVAEIIISFLLMFVISGVATDNRAVGELAGIAVGMTIMVNVFVAGPISGA 214

Query: 247 SMNPVRTLGPAIVTNNFKAIWIYLTAPILGTLCGAGIYTAVKLPDE 289
           SMNP R+LGPA+V   +K IW+Y+  P+LG + G  +Y  ++  D+
Sbjct: 215 SMNPARSLGPALVMGVYKHIWVYIVGPVLGVISGGFVYNLIRFTDK 257

[Close]

BLAST of MS016234 vs. TAIR 10
Match: AT4G19030.1 (NOD26-like major intrinsic protein 1 )

HSP 1 Score: 201.4 bits (511), Expect = 1.0e-51
Identity = 114/275 (41.45%), Postives = 163/275 (59.27%), Query Frame = 0


Query: 32  NGNGKRSLLKSCISFNADDWTNEERGLSS-------KVSCSLSPPPISLARKVGAEFIAT 91
           +GNG  +  +  +  N  D    ++ +         K   SL    +   +K+ AEF+ T
Sbjct: 5   SGNGYGNAREEVVMVNLKDEVEHQQEMEDIHNPRPLKKQDSLLSVSVPFLQKLIAEFLGT 64

Query: 92  LMLIFAGTATAIVNQKTQGTETLIGLAASTGLAVMIVILSTGHISGAHLNPAITIAFAAL 151
             L+F G A+ +VN +     TL G+A   GL +M++I S GHISGAH+NPA+TIAFA+ 
Sbjct: 65  YFLVFTGCASVVVNMQNDNVVTLPGIAIVWGLTIMVLIYSLGHISGAHINPAVTIAFASC 124

Query: 152 KHFPWKHVPVYIGAQMVASLCASFALKWIF-----------DPIMGGGVTVPSVGYEQAF 211
             FP K VP Y+ +Q++ S  A+  L+ +F           D  +G     P     QAF
Sbjct: 125 GRFPLKQVPAYVISQVIGSTLAAATLRLLFGLDHDVCSGKHDVFIGSS---PVGSDLQAF 184

Query: 212 ALEFIISFNLMFVITAVATDTRAVGDLAGIAVGATVMLNVLIAGETTGASMNPVRTLGPA 271
            +EFI++F LMF+I+ VATD RA+G+LAG+A+G+TV+LNVLIA   + ASMNP R+LGPA
Sbjct: 185 TMEFIVTFYLMFIISGVATDNRAIGELAGLAIGSTVLLNVLIAAPVSSASMNPGRSLGPA 244

Query: 272 IVTNNFKAIWIYLTAPILGTLCGAGIYTAVKLPDE 289
           +V   +K IWIYL AP LG + GA +Y  V+  D+
Sbjct: 245 LVYGCYKGIWIYLVAPTLGAIAGAWVYNTVRYTDK 276

The following BLAST results are available for this feature:

BLAST of MS016234 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022153403.1	1.2e-160	97.37	aquaporin NIP6-1 [Momordica charantia]	[more]
XP_008454166.1	4.4e-134	84.97	PREDICTED: aquaporin NIP6-1 [Cucumis melo] >TYK29615.1 aquaporin NIP6-1 [Cucumis...	[more]
XP_004152170.1	5.8e-134	84.31	aquaporin NIP6-1 [Cucumis sativus] >KGN52947.1 hypothetical protein Csa_015162 [...	[more]
KAA0044488.1	5.8e-134	84.64	aquaporin NIP6-1 [Cucumis melo var. makuwa]	[more]
XP_038876197.1	2.9e-133	83.99	aquaporin NIP6-1 [Benincasa hispida]	[more]

BLAST of MS016234 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SAI4	2.6e-113	71.66	Aquaporin NIP6-1 OS=Arabidopsis thaliana OX=3702 GN=NIP6-1 PE=1 SV=1	[more]
Q9SV84	9.7e-92	60.07	Probable aquaporin NIP5-1 OS=Arabidopsis thaliana OX=3702 GN=NIP5-1 PE=2 SV=1	[more]
Q0IWF3	2.8e-83	55.23	Aquaporin NIP3-1 OS=Oryza sativa subsp. japonica OX=39947 GN=NIP3-1 PE=2 SV=2	[more]
Q9ATN1	2.2e-80	56.95	Aquaporin NIP3-1 OS=Zea mays OX=4577 GN=NIP3-1 PE=2 SV=1	[more]
Q7EYH7	7.0e-58	52.53	Aquaporin NIP3-2 OS=Oryza sativa subsp. japonica OX=39947 GN=NIP3-2 PE=2 SV=1	[more]

BLAST of MS016234 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DHD3	6.0e-161	97.37	aquaporin NIP6-1 OS=Momordica charantia OX=3673 GN=LOC111020915 PE=3 SV=1	[more]
A0A5D3E2S7	2.1e-134	84.97	Aquaporin NIP6-1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G002...	[more]
A0A1S3BY36	2.1e-134	84.97	aquaporin NIP6-1 OS=Cucumis melo OX=3656 GN=LOC103494651 PE=3 SV=1	[more]
A0A5A7TNN6	2.8e-134	84.64	Aquaporin NIP6-1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G0022...	[more]
A0A0A0KX08	2.8e-134	84.31	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G007030 PE=3 SV=1	[more]

BLAST of MS016234 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G80760.1	1.9e-114	71.66	NOD26-like intrinsic protein 6;1	[more]
AT4G10380.1	6.9e-93	60.07	NOD26-like intrinsic protein 5;1	[more]
AT5G37820.1	6.3e-54	48.10	NOD26-like intrinsic protein 4;2	[more]
AT5G37810.1	1.1e-53	48.23	NOD26-like intrinsic protein 4;1	[more]
AT4G19030.1	1.0e-51	41.45	NOD26-like major intrinsic protein 1	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000425	Major intrinsic protein	PRINTS	PR00783	MINTRINSICP	coord: 263..283 score: 31.1 coord: 75..94 score: 39.01 coord: 195..213 score: 27.17 coord: 114..138 score: 46.81 coord: 226..248 score: 40.23 coord: 151..170 score: 25.95
IPR000425	Major intrinsic protein	PFAM	PF00230	MIP	coord: 72..280 e-value: 4.4E-50 score: 170.5
IPR000425	Major intrinsic protein	CDD	cd00333	MIP	coord: 75..280 e-value: 1.2228E-53 score: 172.823
IPR023271	Aquaporin-like	GENE3D	1.20.1080.10	Glycerol uptake facilitator protein.	coord: 42..292 e-value: 1.5E-66 score: 225.9
IPR023271	Aquaporin-like	SUPERFAMILY	81338	Aquaporin-like	coord: 72..289
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..28
None	No IPR available	PANTHER	PTHR45724:SF41	BNAA02G36290D PROTEIN	coord: 7..292
IPR034294	Aquaporin transporter	PANTHER	PTHR45724	AQUAPORIN NIP2-1	coord: 7..292
IPR022357	Major intrinsic protein, conserved site	PROSITE	PS00221	MIP	coord: 132..140

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS016234.1	MS016234.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055085	transmembrane transport
cellular_component	GO:0016020	membrane
molecular_function	GO:0015267	channel activity