MS016215 (gene) Bitter gourd (TR) v1

Overview


Name	MS016215
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	phosphatidylinositol-glycan biosynthesis class F protein
Location	scaffold9_2: 1118952 .. 1121887 (+)
RNA-Seq Expression	MS016215
Synteny	MS016215

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GTCATGGCGACGGAGAAGGAGATGGCCAGTAAAGCCCCGACTGCCGTATCGATTGCGGAAGCCTTCCTCGTCCACCTGATCTGCGGCTTGGGGCTAGCCCTAGCTCTCTGGATTGCTCGATACATCTACTCCACCGATCTCATCTCCAATCCTTCTCATACTCTCTTCTTGATTTGGGTATTTTTTCTCTCAATTCTCTTCTTGATTCCGTTTCCTAATTTACTCCCTGCATTTTGAAAGATTTTTCTTCTTTCTCTCTGTTCAGGCTCTTGAGTGTCCGATTGTCATCCTTCTTTACAGCCGCTACCGTCTGGACCGAGAACAATGCTCGGTAAGGTTGACTCTCTCTTGTTTCACGTTAAAGTGTATCGGTTGTTCTGAAATCAGATAAGAAATTTTGAAAAACTTGGACCATCTTCTGTGCGAATTTGCAGTACCTGAAAGCTGTTACGAGAGGCATACTTGGACTCCCTGCTGGTTAGTTGAAGAAATCTCATTACCATTTGGTACTTCTTTCGATTTTCTTTTGTTTAATTAGCTATGAGTGCTTTTGAAGTTGCGCTTCATCTGTCGTTCCAATTGAGCATTCTGAGGATCGAAATATTGTATGCGAAAATCGAAGACGTGTTAAATTCAATAACCATTTCTCGAAATGGATATTGCTCAAATTTTAGGTGAAATGAGATTTCAAATTTGGTGATGTGAGAGTATAGGTATTTTTGTTCCCATGTGCGTCCACGATCACTCTCTGGAGACTACCACGCATTTGTTTTTCTTATTCATAATGATTTGTATTTGTTTGGTTCTTCTCGCTCAACTTGTCATAATGGGATCAATGTGGCCTCTATTATGCAGGTGCTGTTATTAATGCTTTTGGAGCAATAGTTCTAGGCGCTCCCGTTGGTGCTCAGTACGTGAATAAGTGACTGAAAAGCTATATTTTCATCTCCCATGGATTCCAAATTTTGAATCAATTTTTATATTTGATTGGAAAACTTATACCAGAATTTGTACAACAAATAAGGCAAGATCTGCTCCCAGCATCAGCTGGCATTTTATTTTATCACCATTATGGGAACATTACTTTGGTTTTGGTCTTCCTTTTCCAAAATAAAGCTTAATCCAAATTGTGAGTATTTCAAGTGGAATTTTACTTGGGAAGATTAATCGACAGTATCATCAGTCATGATTTGCTGTTTTATAATTATGGATATTGATGTTCCACAGATTTAAGCTTTCAGGCTAAATCACGATAATATTAATACATTTGATACTGAAGTGTTTTTGTGTACATGGTCTGAATACTTCTTCTTGGCACAGATACTTCTCGAAGACCCTTAACTGGTCACTTGTGATGTCATTGTTCAATGTGAGTAAGCTATTCTTAAGATATTTCCTTTGATGACTATATCTCTAACTATATTGTTTCAATTTCTTCCACCTTTTTGAATCCTGTTATATAGTCATTGGCTGTTGTTTTACATCTTTGTGAGATGGATAATGGGACCTATATTTTCTTTTTTGGTTTTCTCAGGTTATGGTTTTAACCCTTTAAATTAGTTAATGACTATTATGAGATTAATGGAAGTGGAGCTTTCTCATATCTGGATATTTTTTTCAGTTTGTTTTGAGGATTATTATCTTCGTATTTACTTTCATGGTAAATGTTGTGCTGTGTTAGAAACCCTTCCCCTTCTCGTATGGATATGTTAGTCCTTTATTGATGGTGGTTTTTGCTCGTCTTGTAGATCGTGCCTTCAGCATGTGTCTTTGGTTCATCATGGACAGATTGGCAGCGCCTATTTGCTTACACAAAGTAAGGCTCCTGATGTGTTATTTATCTCTCTTTACTTGACGTGGATGCATTCTTCTACTAGCAAAATATTTAGTTTTTAACAAAGGTAAATACAAACTCAGAATAAAATGTTTATGTAAAGGTAGTCGATGCTGTGCAATTGCAGGCCCAATGGAACTATCGATCATATGATATGCATTCCAGCACATGGGGCCATTATTGGAGCCTGGTTTGGCGCATGGCCCATGCCACTTGATTGGGAAAGGCCTTGGCAGGTACTATTTTTCCATACTGCACTTGTGTTTTTGCCTATAAAGCTCGGCCAACAAACCTCATTGACATTAAATAGTGTCATTTGATAATCTCTTGTTTCAAAGTTGATGAACCATAAAATGGTACGTCTGGGCATCCCAAAAAAAATAACGAGTCAAGTTTACTTACCTCTAAAATGTGATCTAAGCCGATAAAAAGTTATATGATAGAGCTTAGTTTTTACTGCATCTATTCGTGACTTCTAGGCATTCAAAGACAAAATAATGAGTAGAGTCCACTTATCTATAACTTGTGAACTTAGCTGATACATACGATAGTACTCAATTCTTTTCGGAACTTATTCATAAATGACAACTCACTACATTTTTTCATGTGACCATGCTGTTATATTGATTTGAATCAGATTTTGGGATATGGTATCAATTTATGTCTATTTGCTACCCATATAGTTCCTTTGACAATTCCATACTTAATGGTTGTCGCCTCTTGCTCTAGTGCTCCTAATCTCGATGGTCTTGATGTGGCATTAGATTAGGTAAATTATGTTTTGAATGAATCCTAAGACCAACTAGAATGCTCTCCTATTACAAACAATAAAGAAACTGACAGATTTAGATAGGAGTGACATCAAATAAACCAAAGTCCCTTGTGTGTGTGAAAATGTTAAACCAATCATGTAAATTCAGGTTATTTTTCTTAGTTCTCTTGCATTGCATCTATGCGAACTCTATTAAATAAAGATCACTTTAATATGCAGGAGTGGCCAATATGTGTGAGTTACGGGGCAGTAATTGGGTACTCGACCTCAATGGTAGCATCCCTGGCTTTTTCACGTGTTCGAAGTGGCTTGCAGCACGTTAAAAGAGAC

mRNA sequence

GTCATGGCGACGGAGAAGGAGATGGCCAGTAAAGCCCCGACTGCCGTATCGATTGCGGAAGCCTTCCTCGTCCACCTGATCTGCGGCTTGGGGCTAGCCCTAGCTCTCTGGATTGCTCGATACATCTACTCCACCGATCTCATCTCCAATCCTTCTCATACTCTCTTCTTGATTTGGGCTCTTGAGTGTCCGATTGTCATCCTTCTTTACAGCCGCTACCGTCTGGACCGAGAACAATGCTCGTACCTGAAAGCTGTTACGAGAGGCATACTTGGACTCCCTGCTGGTGCTGTTATTAATGCTTTTGGAGCAATAGTTCTAGGCGCTCCCGTTGGTGCTCAATACTTCTCGAAGACCCTTAACTGGTCACTTGTGATGTCATTGTTCAATATCGTGCCTTCAGCATGTGTCTTTGGTTCATCATGGACAGATTGGCAGCGCCTATTTGCTTACACAAAGCCCAATGGAACTATCGATCATATGATATGCATTCCAGCACATGGGGCCATTATTGGAGCCTGGTTTGGCGCATGGCCCATGCCACTTGATTGGGAAAGGCCTTGGCAGGAGTGGCCAATATGTGTGAGTTACGGGGCAGTAATTGGGTACTCGACCTCAATGGTAGCATCCCTGGCTTTTTCACGTGTTCGAAGTGGCTTGCAGCACGTTAAAAGAGAC

Coding sequence (CDS)

GTCATGGCGACGGAGAAGGAGATGGCCAGTAAAGCCCCGACTGCCGTATCGATTGCGGAAGCCTTCCTCGTCCACCTGATCTGCGGCTTGGGGCTAGCCCTAGCTCTCTGGATTGCTCGATACATCTACTCCACCGATCTCATCTCCAATCCTTCTCATACTCTCTTCTTGATTTGGGCTCTTGAGTGTCCGATTGTCATCCTTCTTTACAGCCGCTACCGTCTGGACCGAGAACAATGCTCGTACCTGAAAGCTGTTACGAGAGGCATACTTGGACTCCCTGCTGGTGCTGTTATTAATGCTTTTGGAGCAATAGTTCTAGGCGCTCCCGTTGGTGCTCAATACTTCTCGAAGACCCTTAACTGGTCACTTGTGATGTCATTGTTCAATATCGTGCCTTCAGCATGTGTCTTTGGTTCATCATGGACAGATTGGCAGCGCCTATTTGCTTACACAAAGCCCAATGGAACTATCGATCATATGATATGCATTCCAGCACATGGGGCCATTATTGGAGCCTGGTTTGGCGCATGGCCCATGCCACTTGATTGGGAAAGGCCTTGGCAGGAGTGGCCAATATGTGTGAGTTACGGGGCAGTAATTGGGTACTCGACCTCAATGGTAGCATCCCTGGCTTTTTCACGTGTTCGAAGTGGCTTGCAGCACGTTAAAAGAGAC

Protein sequence

VMATEKEMASKAPTAVSIAEAFLVHLICGLGLALALWIARYIYSTDLISNPSHTLFLIWALECPIVILLYSRYRLDREQCSYLKAVTRGILGLPAGAVINAFGAIVLGAPVGAQYFSKTLNWSLVMSLFNIVPSACVFGSSWTDWQRLFAYTKPNGTIDHMICIPAHGAIIGAWFGAWPMPLDWERPWQEWPICVSYGAVIGYSTSMVASLAFSRVRSGLQHVKRD

Homology

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BLAST of MS016215 vs. NCBI nr
Match: XP_022153416.1 (phosphatidylinositol-glycan biosynthesis class F protein [Momordica charantia])

HSP 1 Score: 460.3 bits (1183), Expect = 9.6e-126
Identity = 223/225 (99.11%), Postives = 223/225 (99.11%), Query Frame = 0


Query: 2   MATEKEMASKAPTAVSIAEAFLVHLICGLGLALALWIARYIYSTDLISNPSHTLFLIWAL 61
           MATEKEMASKAPTAVSIAEAFLVHLICGLGLALALWIARYIYSTDLISNPSHTLFLIWAL
Sbjct: 1   MATEKEMASKAPTAVSIAEAFLVHLICGLGLALALWIARYIYSTDLISNPSHTLFLIWAL 60

Query: 62  ECPIVILLYSRYRLDREQCSYLKAVTRGILGLPAGAVINAFGAIVLGAPVGAQYFSKTLN 121
           ECPIVILLYSRYRLDREQCSYLKAVTRGILGLPAGAVINAFGAIVLGAPVGAQYFSKTLN
Sbjct: 61  ECPIVILLYSRYRLDREQCSYLKAVTRGILGLPAGAVINAFGAIVLGAPVGAQYFSKTLN 120

Query: 122 WSLVMSLFNIVPSACVFGSSWTDWQRLFAYTKPNGTIDHMICIPAHGAIIGAWFGAWPMP 181
           WSLVMSLFNIVPSACVFGSSW DWQRLFAYTKPNGTIDHMICIPAHGAIIGAWFGAWPMP
Sbjct: 121 WSLVMSLFNIVPSACVFGSSWMDWQRLFAYTKPNGTIDHMICIPAHGAIIGAWFGAWPMP 180

Query: 182 LDWERPWQEWPICVSYGAVIGYSTSMVASLAFSRVRSGLQHVKRD 227
           LDWERPWQEWPICVSYGAVIGYSTSMVASLAFSRVRSGLQHVK D
Sbjct: 181 LDWERPWQEWPICVSYGAVIGYSTSMVASLAFSRVRSGLQHVKGD 225

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BLAST of MS016215 vs. NCBI nr
Match: XP_038904140.1 (phosphatidylinositol-glycan biosynthesis class F protein [Benincasa hispida])

HSP 1 Score: 401.0 bits (1029), Expect = 6.9e-108
Identity = 191/222 (86.04%), Postives = 204/222 (91.89%), Query Frame = 0


Query: 5   EKEMASKAPTAVSIAEAFLVHLICGLGLALALWIARYIYSTDLISNPSHTLFLIWALECP 64
           +KEMA +A   +SI+EAF +HLIC LGLALA WIARYIYSTDLIS+PS TLFLI A+ECP
Sbjct: 5   KKEMACEASIPISISEAFFLHLICALGLALAFWIARYIYSTDLISDPSRTLFLILAIECP 64

Query: 65  IVILLYSRYRLDREQCSYLKAVTRGILGLPAGAVINAFGAIVLGAPVGAQYFSKTLNWSL 124
           IVILLYSRYR DR+QCSYLKAV RG+LGLPAGAVINAFGAIVLGAPVGAQYF KTLNWSL
Sbjct: 65  IVILLYSRYRTDRQQCSYLKAVARGLLGLPAGAVINAFGAIVLGAPVGAQYFVKTLNWSL 124

Query: 125 VMSLFNIVPSACVFGSSWTDWQRLFAYTKPNGTIDHMICIPAHGAIIGAWFGAWPMPLDW 184
           VMSLFNIVPSACVFGSSW DWQRLFAYTKPNGTIDHMICIPAHGAIIGAWFGAWPMPLDW
Sbjct: 125 VMSLFNIVPSACVFGSSWRDWQRLFAYTKPNGTIDHMICIPAHGAIIGAWFGAWPMPLDW 184

Query: 185 ERPWQEWPICVSYGAVIGYSTSMVASLAFSRVRSGLQHVKRD 227
           ERPWQEWPICVSYGA++GYS +MVASL FS +R G QHVKRD
Sbjct: 185 ERPWQEWPICVSYGAILGYSIAMVASLVFSHLR-GSQHVKRD 225

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BLAST of MS016215 vs. NCBI nr
Match: XP_022949007.1 (phosphatidylinositol-glycan biosynthesis class F protein [Cucurbita moschata])

HSP 1 Score: 399.8 bits (1026), Expect = 1.5e-107
Identity = 195/226 (86.28%), Postives = 205/226 (90.71%), Query Frame = 0


Query: 2   MATE-KEMASKAPTAVSIAEAFLVHLICGLGLALALWIARYIYSTDLISNPSHTLFLIWA 61
           M TE KEMASKA  +VSIAEAF VHLICGLGLALA  IAR++YS DLIS+PS TLFLI  
Sbjct: 1   METEKKEMASKASPSVSIAEAFFVHLICGLGLALAFLIARHVYSADLISDPSRTLFLILI 60

Query: 62  LECPIVILLYSRYRLDREQCSYLKAVTRGILGLPAGAVINAFGAIVLGAPVGAQYFSKTL 121
           +ECPIVILLYSRYR DREQCSYLKAV RGILGL AGA+INAFGAIVLGAP+GAQY  KTL
Sbjct: 61  VECPIVILLYSRYRTDREQCSYLKAVARGILGLLAGAIINAFGAIVLGAPIGAQYLLKTL 120

Query: 122 NWSLVMSLFNIVPSACVFGSSWTDWQRLFAYTKPNGTIDHMICIPAHGAIIGAWFGAWPM 181
           NWSLVMS F++VPSACVFGSSW DWQRLFAYTKPNGTIDHMICIPAHGAIIGAWFGAWPM
Sbjct: 121 NWSLVMSSFSVVPSACVFGSSWMDWQRLFAYTKPNGTIDHMICIPAHGAIIGAWFGAWPM 180

Query: 182 PLDWERPWQEWPICVSYGAVIGYSTSMVASLAFSRVRSGLQHVKRD 227
           PLDWERPWQEWPICVSYGAVIGYS +MVASLAFS  RSGLQHVKRD
Sbjct: 181 PLDWERPWQEWPICVSYGAVIGYSIAMVASLAFSHARSGLQHVKRD 226

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BLAST of MS016215 vs. NCBI nr
Match: KAG6606849.1 (Phosphatidylinositol-glycan biosynthesis class F protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 396.4 bits (1017), Expect = 1.7e-106
Identity = 194/227 (85.46%), Postives = 205/227 (90.31%), Query Frame = 0


Query: 1   VMATE-KEMASKAPTAVSIAEAFLVHLICGLGLALALWIARYIYSTDLISNPSHTLFLIW 60
           VM TE KEMA KA  +VSIAEAF VHLICGLGLALA  IAR++YS DLIS+PS TLFLI 
Sbjct: 55  VMETEKKEMARKASPSVSIAEAFFVHLICGLGLALAFLIARHVYSADLISDPSRTLFLIL 114

Query: 61  ALECPIVILLYSRYRLDREQCSYLKAVTRGILGLPAGAVINAFGAIVLGAPVGAQYFSKT 120
            +ECPIVILLYSRYR DREQCSYLKAV RGILGL AGA+INAFGAIVLGAP+GAQY SKT
Sbjct: 115 IVECPIVILLYSRYRTDREQCSYLKAVARGILGLLAGAIINAFGAIVLGAPIGAQYLSKT 174

Query: 121 LNWSLVMSLFNIVPSACVFGSSWTDWQRLFAYTKPNGTIDHMICIPAHGAIIGAWFGAWP 180
           LNWSLVMS F++VPSACVFGSSW DWQRLFA +KPNGTIDHMICIPAHGAIIGAWFGAWP
Sbjct: 175 LNWSLVMSSFSVVPSACVFGSSWMDWQRLFACSKPNGTIDHMICIPAHGAIIGAWFGAWP 234

Query: 181 MPLDWERPWQEWPICVSYGAVIGYSTSMVASLAFSRVRSGLQHVKRD 227
           MPLDWERPWQEWPICVSYGAVIGYS +MVASLAFS  RSGLQHVKRD
Sbjct: 235 MPLDWERPWQEWPICVSYGAVIGYSIAMVASLAFSHARSGLQHVKRD 281

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BLAST of MS016215 vs. NCBI nr
Match: XP_022998495.1 (phosphatidylinositol-glycan biosynthesis class F protein [Cucurbita maxima])

HSP 1 Score: 396.0 bits (1016), Expect = 2.2e-106
Identity = 193/226 (85.40%), Postives = 202/226 (89.38%), Query Frame = 0


Query: 2   MATE-KEMASKAPTAVSIAEAFLVHLICGLGLALALWIARYIYSTDLISNPSHTLFLIWA 61
           M TE KEM SKA   VSIAEAF VHLICGLGLALA  IAR++YS DLIS+PS TLFLI  
Sbjct: 1   METEKKEMTSKASPPVSIAEAFFVHLICGLGLALAFLIARHVYSADLISDPSRTLFLILI 60

Query: 62  LECPIVILLYSRYRLDREQCSYLKAVTRGILGLPAGAVINAFGAIVLGAPVGAQYFSKTL 121
           +ECPIVILLYSRYR DREQCSYLKA+ RGILGL AGA+INAFGAIVLGAP+GAQY  KTL
Sbjct: 61  VECPIVILLYSRYRTDREQCSYLKAMARGILGLLAGAIINAFGAIVLGAPIGAQYLLKTL 120

Query: 122 NWSLVMSLFNIVPSACVFGSSWTDWQRLFAYTKPNGTIDHMICIPAHGAIIGAWFGAWPM 181
           NWSLVMS F +VPSACVFGSSW DWQRLFAYTKPNGTIDHMICIPAHGAIIGAWFGAWPM
Sbjct: 121 NWSLVMSSFGVVPSACVFGSSWMDWQRLFAYTKPNGTIDHMICIPAHGAIIGAWFGAWPM 180

Query: 182 PLDWERPWQEWPICVSYGAVIGYSTSMVASLAFSRVRSGLQHVKRD 227
           PLDWERPWQEWPICVSYGAVIGYS +MVASLAFS  RSGLQHVKRD
Sbjct: 181 PLDWERPWQEWPICVSYGAVIGYSIAMVASLAFSHARSGLQHVKRD 226

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BLAST of MS016215 vs. ExPASy Swiss-Prot
Match: Q9Y7P2 (Uncharacterized protein C1450.15 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPCC1450.15 PE=3 SV=2)

HSP 1 Score: 88.6 bits (218), Expect = 9.9e-17
Identity = 58/171 (33.92%), Postives = 86/171 (50.29%), Query Frame = 0


Query: 41  YIYSTDLISNPSHTL---FLIWALECPIVILLYSRYRLDREQCSYLKAVTRGILGLPAGA 100
           Y+    LI NP   L   F IW +   + I + S     R   +  K +  G   +  G+
Sbjct: 326 YLSLNCLIENPYRMLRNTFPIWFIMQTLQIYIQS----PRPPLT-PKRLLAGAASMLIGS 385

Query: 101 VINAFGAIVLGAPVGAQYFSKTLNWSLVMSLFNIVPSACVFGSSWTDWQRLFAYTKPNGT 160
           ++ +F  +  GAP+    F  T   +L +S+F + P A     +   WQR F   K    
Sbjct: 386 LLISFILVAFGAPL-LHDFHLTYFCALTLSVFTVYPLASTLAFNTEQWQR-FLTLKSFNV 445

Query: 161 IDHMICIPAHGAIIGAWFGAWPMPLDWERPWQEWPICVSYGAVIGYSTSMV 209
           I  M  + + G IIGAWFGA+P+PLDW+RPWQ WPI +  GA +GY+ + +
Sbjct: 446 IGSM-QLRSWGPIIGAWFGAFPIPLDWDRPWQAWPITIVIGAFLGYAFAAI 488

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BLAST of MS016215 vs. ExPASy Swiss-Prot
Match: O09101 (Phosphatidylinositol-glycan biosynthesis class F protein OS=Mus musculus OX=10090 GN=Pigf PE=1 SV=1)

HSP 1 Score: 87.8 bits (216), Expect = 1.7e-16
Identity = 39/106 (36.79%), Postives = 60/106 (56.60%), Query Frame = 0


Query: 105 IVLGAPVGAQYFSKTLNWSLVMSLFNIVPSACVFGSSWTDWQRLFAYTKPNGTIDHMICI 164
           ++ GAP+  +   +T  +++V+S F  VP  C+ G +   W R+F+        ++ + I
Sbjct: 99  VLYGAPL-IELVLETFLFAVVLSTFTTVPCLCLLGPNLKAWLRVFSRNGVTSIWENSLQI 158

Query: 165 PAHGAIIGAWFGAWPMPLDWERPWQEWPICVSYGAVIGYSTSMVAS 211
               +  GAW GA+P+PLDWERPWQ WPI  + GA  GY   +V S
Sbjct: 159 TTISSFTGAWLGAFPIPLDWERPWQVWPISCTLGATFGYVAGLVIS 203

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BLAST of MS016215 vs. ExPASy Swiss-Prot
Match: Q07326 (Phosphatidylinositol-glycan biosynthesis class F protein OS=Homo sapiens OX=9606 GN=PIGF PE=1 SV=1)

HSP 1 Score: 84.7 bits (208), Expect = 1.4e-15
Identity = 53/203 (26.11%), Postives = 95/203 (46.80%), Query Frame = 0


Query: 18  IAEAFLVHLICGLGLALALWI-ARYIYSTDLISNPSHTLFLIWALECPIVILLY------ 77
           I      HL+C   + L+++I + ++ +  ++      L +       + ++LY      
Sbjct: 6   IKRLLYTHLLCIFSIILSVFIPSLFLENFSILETHLTWLCICSGFVTAVNLVLYLVVKPN 65

Query: 78  ---SRYRLDREQCSYLKAVTRGILGLPAGAVINAFGAIVLGAPVGAQYFSKTLNWSLVMS 137
               R  L  +   +LK     ++   +  VI     ++ GAP+  +   +T  +++++S
Sbjct: 66  TSSKRSSLSHKVTGFLKCCIYFLMSCFSFHVI----FVLYGAPL-IELALETFLFAVILS 125

Query: 138 LFNIVPSACVFGSSWTDWQRLFAYTKPNGTIDHMICIPAHGAIIGAWFGAWPMPLDWERP 197
            F  VP  C+ G +   W R+F+        ++ + I    + +GAW GA P+PLDWERP
Sbjct: 126 TFTTVPCLCLLGPNLKAWLRVFSRNGVTSIWENSLQITTISSFVGAWLGALPIPLDWERP 185

Query: 198 WQEWPICVSYGAVIGYSTSMVAS 211
           WQ WPI  + GA  GY   +V S
Sbjct: 186 WQVWPISCTLGATFGYVAGLVIS 203

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BLAST of MS016215 vs. ExPASy Swiss-Prot
Match: Q6C741 (Glycosylphosphatidylinositol anchor biosynthesis protein 11 OS=Yarrowia lipolytica (strain CLIB 122 / E 150) OX=284591 GN=GPI11 PE=3 SV=1)

HSP 1 Score: 72.4 bits (176), Expect = 7.4e-12
Identity = 57/233 (24.46%), Postives = 99/233 (42.49%), Query Frame = 0


Query: 2   MATEKEMASKA--PTAVSIAEAFLVHLICGLGLALALWIARYIYSTDLISNPSHT----- 61
           MAT K   +KA  P  V    + ++ ++ G    L L   R+I+S  +  +P+       
Sbjct: 1   MATRKAAKTKANGPAHVPSGPSQVLAVVYG---TLLLVYLRFIFSAGITHDPAKVMTQAL 60

Query: 62  --LFLIWALECPIVILLYSRYRLDREQCSYLKAVTRGILGLPAGAVINAFGAIVLGAPVG 121
             L L+    C +V+      ++  +  + + A    +        +  FG +VL     
Sbjct: 61  PGLLLLHMGYCVVVLGNKPGRKIGTDVSTAMIAAALSVF-----FSVIIFGLLVLFGAPA 120

Query: 122 AQYFSKTLNWSLVMSLFNIVPSACVFGSSWTDWQRLFAYTKPNGTIDHMICIPAHGAIIG 181
                 T   ++ MS+  ++P    +      W  + A  +P   +DH+        +IG
Sbjct: 121 ISLVHNTFVCAMHMSILAVLPLFFTYHLDSKVWADIIAMRRP---LDHVYAASV-CTLIG 180

Query: 182 AWFGAWPMPLDWERPWQEWPICVSYGAVIGYSTSMVASLAFSRVRSGLQHVKR 226
           AW GA P+P DW+RPWQ+WPI +  GA +GY    +  +A    +S     K+
Sbjct: 181 AWLGAIPIPYDWDRPWQQWPITILAGAYLGYFVGTLGGIALELTKSLCSKTKK 221

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BLAST of MS016215 vs. ExPASy Swiss-Prot
Match: Q6BHK4 (Glycosylphosphatidylinositol anchor biosynthesis protein 11 OS=Debaryomyces hansenii (strain ATCC 36239 / CBS 767 / BCRC 21394 / JCM 1990 / NBRC 0083 / IGC 2968) OX=284592 GN=GPI11 PE=3 SV=2)

HSP 1 Score: 72.0 bits (175), Expect = 9.6e-12
Identity = 40/113 (35.40%), Postives = 61/113 (53.98%), Query Frame = 0


Query: 105 IVLGAPVGAQYFSKTLNWSLVMSLFNIVPSACVFGSSWTDWQRLFAYTKPNGTID----H 164
           I+LGAP+ A +  +T   S+ +SL    PS  ++     D++ L  +   +G  +    +
Sbjct: 127 ILLGAPL-ASHLKETYLLSIHLSLLIFYPSLVLYK---YDYKSLVKFLDADGVYNAIAKN 186

Query: 165 MICIPAHGAIIGAWFGAWPMPLDWERPWQEWPICVSYGAVIGYSTSMVASLAF 214
            I + A  A+IG WFG  P+PLDW+R WQ+WPI +  GA IG     +A   F
Sbjct: 187 QILLSAVLAVIGTWFGVIPIPLDWDRDWQQWPITLLTGAYIGSFVGGIACFLF 235

[Close]

BLAST of MS016215 vs. ExPASy TrEMBL
Match: A0A6J1DKK8 (phosphatidylinositol-glycan biosynthesis class F protein OS=Momordica charantia OX=3673 GN=LOC111020928 PE=3 SV=1)

HSP 1 Score: 460.3 bits (1183), Expect = 4.6e-126
Identity = 223/225 (99.11%), Postives = 223/225 (99.11%), Query Frame = 0


Query: 2   MATEKEMASKAPTAVSIAEAFLVHLICGLGLALALWIARYIYSTDLISNPSHTLFLIWAL 61
           MATEKEMASKAPTAVSIAEAFLVHLICGLGLALALWIARYIYSTDLISNPSHTLFLIWAL
Sbjct: 1   MATEKEMASKAPTAVSIAEAFLVHLICGLGLALALWIARYIYSTDLISNPSHTLFLIWAL 60

Query: 62  ECPIVILLYSRYRLDREQCSYLKAVTRGILGLPAGAVINAFGAIVLGAPVGAQYFSKTLN 121
           ECPIVILLYSRYRLDREQCSYLKAVTRGILGLPAGAVINAFGAIVLGAPVGAQYFSKTLN
Sbjct: 61  ECPIVILLYSRYRLDREQCSYLKAVTRGILGLPAGAVINAFGAIVLGAPVGAQYFSKTLN 120

Query: 122 WSLVMSLFNIVPSACVFGSSWTDWQRLFAYTKPNGTIDHMICIPAHGAIIGAWFGAWPMP 181
           WSLVMSLFNIVPSACVFGSSW DWQRLFAYTKPNGTIDHMICIPAHGAIIGAWFGAWPMP
Sbjct: 121 WSLVMSLFNIVPSACVFGSSWMDWQRLFAYTKPNGTIDHMICIPAHGAIIGAWFGAWPMP 180

Query: 182 LDWERPWQEWPICVSYGAVIGYSTSMVASLAFSRVRSGLQHVKRD 227
           LDWERPWQEWPICVSYGAVIGYSTSMVASLAFSRVRSGLQHVK D
Sbjct: 181 LDWERPWQEWPICVSYGAVIGYSTSMVASLAFSRVRSGLQHVKGD 225

[Close]

BLAST of MS016215 vs. ExPASy TrEMBL
Match: A0A6J1GAT8 (phosphatidylinositol-glycan biosynthesis class F protein OS=Cucurbita moschata OX=3662 GN=LOC111452477 PE=3 SV=1)

HSP 1 Score: 399.8 bits (1026), Expect = 7.4e-108
Identity = 195/226 (86.28%), Postives = 205/226 (90.71%), Query Frame = 0


Query: 2   MATE-KEMASKAPTAVSIAEAFLVHLICGLGLALALWIARYIYSTDLISNPSHTLFLIWA 61
           M TE KEMASKA  +VSIAEAF VHLICGLGLALA  IAR++YS DLIS+PS TLFLI  
Sbjct: 1   METEKKEMASKASPSVSIAEAFFVHLICGLGLALAFLIARHVYSADLISDPSRTLFLILI 60

Query: 62  LECPIVILLYSRYRLDREQCSYLKAVTRGILGLPAGAVINAFGAIVLGAPVGAQYFSKTL 121
           +ECPIVILLYSRYR DREQCSYLKAV RGILGL AGA+INAFGAIVLGAP+GAQY  KTL
Sbjct: 61  VECPIVILLYSRYRTDREQCSYLKAVARGILGLLAGAIINAFGAIVLGAPIGAQYLLKTL 120

Query: 122 NWSLVMSLFNIVPSACVFGSSWTDWQRLFAYTKPNGTIDHMICIPAHGAIIGAWFGAWPM 181
           NWSLVMS F++VPSACVFGSSW DWQRLFAYTKPNGTIDHMICIPAHGAIIGAWFGAWPM
Sbjct: 121 NWSLVMSSFSVVPSACVFGSSWMDWQRLFAYTKPNGTIDHMICIPAHGAIIGAWFGAWPM 180

Query: 182 PLDWERPWQEWPICVSYGAVIGYSTSMVASLAFSRVRSGLQHVKRD 227
           PLDWERPWQEWPICVSYGAVIGYS +MVASLAFS  RSGLQHVKRD
Sbjct: 181 PLDWERPWQEWPICVSYGAVIGYSIAMVASLAFSHARSGLQHVKRD 226

[Close]

BLAST of MS016215 vs. ExPASy TrEMBL
Match: A0A6J1KCP2 (phosphatidylinositol-glycan biosynthesis class F protein OS=Cucurbita maxima OX=3661 GN=LOC111493110 PE=3 SV=1)

HSP 1 Score: 396.0 bits (1016), Expect = 1.1e-106
Identity = 193/226 (85.40%), Postives = 202/226 (89.38%), Query Frame = 0


Query: 2   MATE-KEMASKAPTAVSIAEAFLVHLICGLGLALALWIARYIYSTDLISNPSHTLFLIWA 61
           M TE KEM SKA   VSIAEAF VHLICGLGLALA  IAR++YS DLIS+PS TLFLI  
Sbjct: 1   METEKKEMTSKASPPVSIAEAFFVHLICGLGLALAFLIARHVYSADLISDPSRTLFLILI 60

Query: 62  LECPIVILLYSRYRLDREQCSYLKAVTRGILGLPAGAVINAFGAIVLGAPVGAQYFSKTL 121
           +ECPIVILLYSRYR DREQCSYLKA+ RGILGL AGA+INAFGAIVLGAP+GAQY  KTL
Sbjct: 61  VECPIVILLYSRYRTDREQCSYLKAMARGILGLLAGAIINAFGAIVLGAPIGAQYLLKTL 120

Query: 122 NWSLVMSLFNIVPSACVFGSSWTDWQRLFAYTKPNGTIDHMICIPAHGAIIGAWFGAWPM 181
           NWSLVMS F +VPSACVFGSSW DWQRLFAYTKPNGTIDHMICIPAHGAIIGAWFGAWPM
Sbjct: 121 NWSLVMSSFGVVPSACVFGSSWMDWQRLFAYTKPNGTIDHMICIPAHGAIIGAWFGAWPM 180

Query: 182 PLDWERPWQEWPICVSYGAVIGYSTSMVASLAFSRVRSGLQHVKRD 227
           PLDWERPWQEWPICVSYGAVIGYS +MVASLAFS  RSGLQHVKRD
Sbjct: 181 PLDWERPWQEWPICVSYGAVIGYSIAMVASLAFSHARSGLQHVKRD 226

[Close]

BLAST of MS016215 vs. ExPASy TrEMBL
Match: A0A5A7T0F5 (Glycosylphosphatidylinositol anchor biosynthesis protein 11 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1199G00080 PE=3 SV=1)

HSP 1 Score: 377.9 bits (969), Expect = 3.0e-101
Identity = 180/222 (81.08%), Postives = 195/222 (87.84%), Query Frame = 0


Query: 5   EKEMASKAPTAVSIAEAFLVHLICGLGLALALWIARYIYSTDLISNPSHTLFLIWALECP 64
           +KEMASK+ T +SI EAF +HLI  LGLALA WIA YI+STDLIS+PS TLF I  ++ P
Sbjct: 5   KKEMASKSSTPISIPEAFFLHLISTLGLALAFWIAHYIFSTDLISDPSRTLFFILVVQSP 64

Query: 65  IVILLYSRYRLDREQCSYLKAVTRGILGLPAGAVINAFGAIVLGAPVGAQYFSKTLNWSL 124
           IVILLYSRYR DR QCSYLKAV RG+LGLP GA+INAFGAIVLGAP+GAQYF KTLNWSL
Sbjct: 65  IVILLYSRYRTDRHQCSYLKAVARGLLGLPTGAIINAFGAIVLGAPIGAQYFLKTLNWSL 124

Query: 125 VMSLFNIVPSACVFGSSWTDWQRLFAYTKPNGTIDHMICIPAHGAIIGAWFGAWPMPLDW 184
           VMSLFNIVPSACVFGSSW DWQRLFAYTKP GTIDHMICIPAHGAIIGAWFGAWPMPLDW
Sbjct: 125 VMSLFNIVPSACVFGSSWIDWQRLFAYTKPIGTIDHMICIPAHGAIIGAWFGAWPMPLDW 184

Query: 185 ERPWQEWPICVSYGAVIGYSTSMVASLAFSRVRSGLQHVKRD 227
           ERPWQEWPICV+YGA++GYS +M ASL  S  R GLQHVKRD
Sbjct: 185 ERPWQEWPICVTYGAILGYSIAMAASLVLSHQR-GLQHVKRD 225

[Close]

BLAST of MS016215 vs. ExPASy TrEMBL
Match: A0A0A0L9Y3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G710220 PE=3 SV=1)

HSP 1 Score: 371.7 bits (953), Expect = 2.2e-99
Identity = 178/222 (80.18%), Postives = 193/222 (86.94%), Query Frame = 0


Query: 5   EKEMASKAPTAVSIAEAFLVHLICGLGLALALWIARYIYSTDLISNPSHTLFLIWALECP 64
           +KEMAS + T +SI EAF +HLI  L LALA WIA YI+ST LIS+PS TLFLI  ++ P
Sbjct: 5   KKEMASTSSTPISIPEAFFLHLISALALALAFWIAHYIFSTHLISDPSLTLFLILVVQSP 64

Query: 65  IVILLYSRYRLDREQCSYLKAVTRGILGLPAGAVINAFGAIVLGAPVGAQYFSKTLNWSL 124
           IVILLYSRYR DR QCSY KAV RG+LGLPAGA+INAFGAIVLGAP+GAQYF KTLNWSL
Sbjct: 65  IVILLYSRYRTDRHQCSYFKAVARGLLGLPAGAIINAFGAIVLGAPIGAQYFLKTLNWSL 124

Query: 125 VMSLFNIVPSACVFGSSWTDWQRLFAYTKPNGTIDHMICIPAHGAIIGAWFGAWPMPLDW 184
           VMSLFNIVPSACVFGSSW DWQRLFAYTKP GTIDHMICIPAHGAIIGAWFGAWPMPLDW
Sbjct: 125 VMSLFNIVPSACVFGSSWIDWQRLFAYTKPIGTIDHMICIPAHGAIIGAWFGAWPMPLDW 184

Query: 185 ERPWQEWPICVSYGAVIGYSTSMVASLAFSRVRSGLQHVKRD 227
           ERPWQEWPICV+YGA++GYS +M ASL  S  R GLQHVKRD
Sbjct: 185 ERPWQEWPICVTYGAILGYSIAMAASLVLSHQR-GLQHVKRD 225

[Close]

BLAST of MS016215 vs. TAIR 10
Match: AT1G16040.1 (FUNCTIONS IN: molecular_function unknown; INVOLVED IN: GPI anchor biosynthetic process; LOCATED IN: integral to membrane, endoplasmic reticulum membrane; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: GPI biosynthesis protein Pig-F (InterPro:IPR009580); Has 280 Blast hits to 280 proteins in 133 species: Archae - 0; Bacteria - 0; Metazoa - 113; Fungi - 111; Plants - 44; Viruses - 0; Other Eukaryotes - 12 (source: NCBI BLink). )

HSP 1 Score: 245.7 bits (626), Expect = 3.5e-65
Identity = 111/196 (56.63%), Postives = 145/196 (73.98%), Query Frame = 0


Query: 16  VSIAEAFLVHLICGLGLALALWIARYIYSTDLISNPSHTLFLIWALECPIVILLYSRYRL 75
           +S   AF V++I GL L     + R  YS DLIS+P+ TL L+W +E PIV+++YS +R 
Sbjct: 18  ISTWGAFAVYVITGLFLIFGFHVVRNKYSVDLISDPTLTLRLLWIIEFPIVVIIYSLFRR 77

Query: 76  DREQCSYLKAVTRGILGLPAGAVINAFGAIVLGAPVGAQYFSKTLNWSLVMSLFNIVPSA 135
           + E+CSY +AV R ++GL AGA+INA GA+ LGAP+G Q  SKT++WS +MS+F +VP+ 
Sbjct: 78  NPEKCSYFRAVGRSLVGLIAGALINALGAVSLGAPIGMQSLSKTIHWSFLMSVFTVVPAT 137

Query: 136 CVFGSSWTDWQRLFAYTKPNGTIDHMICIPAHGAIIGAWFGAWPMPLDWERPWQEWPICV 195
            V G+SW DW R+FA  KP G I+HM+ +PA+GAIIG WFGAWPMPLDWERPWQEWPICV
Sbjct: 138 AVLGASWIDWHRIFASLKPIGIIEHMLLVPAYGAIIGGWFGAWPMPLDWERPWQEWPICV 197

Query: 196 SYGAVIGYSTSMVASL 212
            YGA+ GY    + SL
Sbjct: 198 CYGAIGGYIGGQMVSL 213

The following BLAST results are available for this feature:

BLAST of MS016215 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022153416.1	9.6e-126	99.11	phosphatidylinositol-glycan biosynthesis class F protein [Momordica charantia]	[more]
XP_038904140.1	6.9e-108	86.04	phosphatidylinositol-glycan biosynthesis class F protein [Benincasa hispida]	[more]
XP_022949007.1	1.5e-107	86.28	phosphatidylinositol-glycan biosynthesis class F protein [Cucurbita moschata]	[more]
KAG6606849.1	1.7e-106	85.46	Phosphatidylinositol-glycan biosynthesis class F protein, partial [Cucurbita arg...	[more]
XP_022998495.1	2.2e-106	85.40	phosphatidylinositol-glycan biosynthesis class F protein [Cucurbita maxima]	[more]

BLAST of MS016215 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9Y7P2	9.9e-17	33.92	Uncharacterized protein C1450.15 OS=Schizosaccharomyces pombe (strain 972 / ATCC...	[more]
O09101	1.7e-16	36.79	Phosphatidylinositol-glycan biosynthesis class F protein OS=Mus musculus OX=1009...	[more]
Q07326	1.4e-15	26.11	Phosphatidylinositol-glycan biosynthesis class F protein OS=Homo sapiens OX=9606...	[more]
Q6C741	7.4e-12	24.46	Glycosylphosphatidylinositol anchor biosynthesis protein 11 OS=Yarrowia lipolyti...	[more]
Q6BHK4	9.6e-12	35.40	Glycosylphosphatidylinositol anchor biosynthesis protein 11 OS=Debaryomyces hans...	[more]

BLAST of MS016215 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DKK8	4.6e-126	99.11	phosphatidylinositol-glycan biosynthesis class F protein OS=Momordica charantia ...	[more]
A0A6J1GAT8	7.4e-108	86.28	phosphatidylinositol-glycan biosynthesis class F protein OS=Cucurbita moschata O...	[more]
A0A6J1KCP2	1.1e-106	85.40	phosphatidylinositol-glycan biosynthesis class F protein OS=Cucurbita maxima OX=...	[more]
A0A5A7T0F5	3.0e-101	81.08	Glycosylphosphatidylinositol anchor biosynthesis protein 11 OS=Cucumis melo var....	[more]
A0A0A0L9Y3	2.2e-99	80.18	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G710220 PE=3 SV=1	[more]

BLAST of MS016215 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G16040.1	3.5e-65	56.63	FUNCTIONS IN: molecular_function unknown; INVOLVED IN: GPI anchor biosynthetic p...	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR009580	GPI biosynthesis protein Pig-F	PFAM	PF06699	PIG-F	coord: 40..210 e-value: 1.7E-41 score: 142.3
None	No IPR available	PANTHER	PTHR43157	PHOSPHATIDYLINOSITOL-GLYCAN BIOSYNTHESIS CLASS F PROTEIN-RELATED	coord: 11..216
None	No IPR available	PANTHER	PTHR43157:SF41	BNAA09G56460D PROTEIN	coord: 11..216

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS016215.1	MS016215.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006506	GPI anchor biosynthetic process
cellular_component	GO:0005789	endoplasmic reticulum membrane