MS016213 (gene) Bitter gourd (TR) v1

BLAST of MS016213 vs. NCBI nr
Match: KAG6581611.1 (4-coumarate--CoA ligase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 985.7 bits (2547), Expect = 1.6e-283
Identity = 495/545 (90.83%), Postives = 517/545 (94.86%), Query Frame = 0

Query: 1   MATETLPNDFIFRSKLPDIYIPKHLPLHSYCLHENAAKIGHRTCLINGVTGDSYTYADVD 60
           MA E L NDFIFRSKLPDIYIP HLPLHSYCLHEN AKIGHRTCLIN VTG+S+TY DVD
Sbjct: 1   MAVEALQNDFIFRSKLPDIYIPNHLPLHSYCLHENLAKIGHRTCLINSVTGESFTYHDVD 60

Query: 61  LTARKVASGLNKLGIAQGDVLMLLLPNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ 120
           L ARKVASGLNKLGIAQGDV+MLLL NSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ
Sbjct: 61  LAARKVASGLNKLGIAQGDVIMLLLQNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ 120

Query: 121 AKGSKAKLIITQASYYDKVKEITEELPDVKIMTVDSPPDGCLSFSDLTQADEREIPAVDI 180
           AKGS AKLI+TQ+SYY+KVKEITEELPDVKIMTVDSPPDGCLSF+DL QADERE+P V+I
Sbjct: 121 AKGSNAKLIVTQSSYYEKVKEITEELPDVKIMTVDSPPDGCLSFADLIQADEREMPTVEI 180

Query: 181 GPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFRNDDVILCVLPLFHI 240
            PDDVVALPYSSGTTGLPKGVMLTH+GLVTSVAQQVDGENPNLY+RN+DVILCVLPLFHI
Sbjct: 181 NPDDVVALPYSSGTTGLPKGVMLTHRGLVTSVAQQVDGENPNLYYRNEDVILCVLPLFHI 240

Query: 241 YSLNSVLLCGLRAGATILILPKFEIASLLQLVQKYRVSIAPVVPPIVLAIAKSPDLDKYD 300
           YSLNSVLLCGLRAG TILI+PKFEI SLLQLVQK++VSIAP+VPPIVLAIAKSPDLDKYD
Sbjct: 241 YSLNSVLLCGLRAGTTILIMPKFEIGSLLQLVQKFKVSIAPIVPPIVLAIAKSPDLDKYD 300

Query: 301 LSSIRVLKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360
           LSSIRV+K GGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG
Sbjct: 301 LSSIRVIKCGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360

Query: 361 ACGTVVRNAEMKIVDTENGASLPRNTPGEICIRGDQIMKGYLNNPEATVATIDADGWLHT 420
           ACGTVVRNAEMKIVDTENG SLPRNTPGEICIRGDQIMKGYLNNPEAT ATID DGWLHT
Sbjct: 361 ACGTVVRNAEMKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420

Query: 421 GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVITDAAVVPMKDEQAGEV 480
           GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAE+EALLLTHP I+DAAVVPMKDE+AGEV
Sbjct: 421 GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAEVEALLLTHPAISDAAVVPMKDEEAGEV 480

Query: 481 PVAFVVKSKSSEVTEDEIKQFISKQQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLA 540
           PVAFVV+ K+SEVTEDEIKQFISK QVVFYKRI R F IDAIPKSPSGKILRKELRAKLA
Sbjct: 481 PVAFVVRLKNSEVTEDEIKQFISK-QVVFYKRIKRAFMIDAIPKSPSGKILRKELRAKLA 540

Query: 541 DVSPN 546
              PN
Sbjct: 541 TGFPN 544

BLAST of MS016213 vs. NCBI nr
Match: XP_022935389.1 (4-coumarate--CoA ligase 1-like [Cucurbita moschata])

HSP 1 Score: 984.6 bits (2544), Expect = 3.5e-283
Identity = 494/545 (90.64%), Postives = 517/545 (94.86%), Query Frame = 0

Query: 1   MATETLPNDFIFRSKLPDIYIPKHLPLHSYCLHENAAKIGHRTCLINGVTGDSYTYADVD 60
           MA E L NDFIFRSKLPDIYIP HLPLHSYCLHEN AKIGHRTCLIN VTG+S+TY DVD
Sbjct: 1   MAVEALQNDFIFRSKLPDIYIPNHLPLHSYCLHENLAKIGHRTCLINSVTGESFTYHDVD 60

Query: 61  LTARKVASGLNKLGIAQGDVLMLLLPNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ 120
           L ARKVASGLNKLGIAQGDV+ML+L NSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ
Sbjct: 61  LAARKVASGLNKLGIAQGDVIMLILQNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ 120

Query: 121 AKGSKAKLIITQASYYDKVKEITEELPDVKIMTVDSPPDGCLSFSDLTQADEREIPAVDI 180
           AKGS AKLI+TQ+SYY+KVKEITEELPDVKIMTVDSPPDGCLSF+DL QADERE+P V+I
Sbjct: 121 AKGSNAKLIVTQSSYYEKVKEITEELPDVKIMTVDSPPDGCLSFADLIQADEREMPTVEI 180

Query: 181 GPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFRNDDVILCVLPLFHI 240
            PDDVVALPYSSGTTGLPKGVMLTH+GLVTSVAQQVDGENPNLY+RN+DVILCVLPLFHI
Sbjct: 181 NPDDVVALPYSSGTTGLPKGVMLTHRGLVTSVAQQVDGENPNLYYRNEDVILCVLPLFHI 240

Query: 241 YSLNSVLLCGLRAGATILILPKFEIASLLQLVQKYRVSIAPVVPPIVLAIAKSPDLDKYD 300
           YSLNSVLLCGLRAG TILI+PKFEI SLLQLVQK++VSIAP+VPPIVLAIAKSPDLDKYD
Sbjct: 241 YSLNSVLLCGLRAGTTILIMPKFEIGSLLQLVQKFKVSIAPIVPPIVLAIAKSPDLDKYD 300

Query: 301 LSSIRVLKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360
           LSSIRV+K GGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG
Sbjct: 301 LSSIRVIKCGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360

Query: 361 ACGTVVRNAEMKIVDTENGASLPRNTPGEICIRGDQIMKGYLNNPEATVATIDADGWLHT 420
           ACGTVVRNAEMKIVDTENG SLPRNTPGEICIRGDQIMKGYLNNPEAT ATID DGWLHT
Sbjct: 361 ACGTVVRNAEMKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420

Query: 421 GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVITDAAVVPMKDEQAGEV 480
           GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAE+EALLLTHP I+DAAVVPMKDE+AGEV
Sbjct: 421 GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAEVEALLLTHPAISDAAVVPMKDEEAGEV 480

Query: 481 PVAFVVKSKSSEVTEDEIKQFISKQQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLA 540
           PVAFVV+ K+SEVTEDEIKQFISK QVVFYKRI R F IDAIPKSPSGKILRKELRAKLA
Sbjct: 481 PVAFVVRLKNSEVTEDEIKQFISK-QVVFYKRIKRAFMIDAIPKSPSGKILRKELRAKLA 540

Query: 541 DVSPN 546
              PN
Sbjct: 541 AGFPN 544

BLAST of MS016213 vs. NCBI nr
Match: XP_023526871.1 (4-coumarate--CoA ligase 1-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 980.3 bits (2533), Expect = 6.6e-282
Identity = 492/545 (90.28%), Postives = 515/545 (94.50%), Query Frame = 0

Query: 1   MATETLPNDFIFRSKLPDIYIPKHLPLHSYCLHENAAKIGHRTCLINGVTGDSYTYADVD 60
           MA E L NDFIFRSKLP IYIP HLPLHSYCLHEN AKIGHRTCLIN VTG+S+TY DVD
Sbjct: 1   MAVEALQNDFIFRSKLPHIYIPNHLPLHSYCLHENLAKIGHRTCLINSVTGESFTYHDVD 60

Query: 61  LTARKVASGLNKLGIAQGDVLMLLLPNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ 120
           L ARKVASGLNKLGIAQGDV+MLLL NSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ
Sbjct: 61  LAARKVASGLNKLGIAQGDVIMLLLQNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ 120

Query: 121 AKGSKAKLIITQASYYDKVKEITEELPDVKIMTVDSPPDGCLSFSDLTQADEREIPAVDI 180
           AKGS AKLI+TQ+SYY+KVKEITEE+PDVKIMTVDSPPDGCLSF+DL QADERE+P V+I
Sbjct: 121 AKGSNAKLIVTQSSYYEKVKEITEEVPDVKIMTVDSPPDGCLSFADLIQADEREMPTVEI 180

Query: 181 GPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFRNDDVILCVLPLFHI 240
            PDDVVALPYSSGTTGLPKGVMLTH+GLVTSVAQQVDGENPNLY+RN+DVILCVLPLFHI
Sbjct: 181 NPDDVVALPYSSGTTGLPKGVMLTHRGLVTSVAQQVDGENPNLYYRNEDVILCVLPLFHI 240

Query: 241 YSLNSVLLCGLRAGATILILPKFEIASLLQLVQKYRVSIAPVVPPIVLAIAKSPDLDKYD 300
           YSLNSVLLCGLRAG TILI+PKFEI SLLQLVQK++VSIAP+VPPIVLAIAKSPDLDKYD
Sbjct: 241 YSLNSVLLCGLRAGTTILIMPKFEIGSLLQLVQKFKVSIAPIVPPIVLAIAKSPDLDKYD 300

Query: 301 LSSIRVLKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360
           LSSIRV+K GGAPLGKELEDTVR KFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG
Sbjct: 301 LSSIRVIKCGGAPLGKELEDTVRTKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360

Query: 361 ACGTVVRNAEMKIVDTENGASLPRNTPGEICIRGDQIMKGYLNNPEATVATIDADGWLHT 420
           ACGTVVRNAEMKIVDTENG SLPRNTPGEICIRGDQIMKGYLNNPEAT ATID DGWLHT
Sbjct: 361 ACGTVVRNAEMKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420

Query: 421 GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVITDAAVVPMKDEQAGEV 480
           GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAE+EALLLTHP I+DAAVVPMKDE+AGEV
Sbjct: 421 GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAEVEALLLTHPAISDAAVVPMKDEEAGEV 480

Query: 481 PVAFVVKSKSSEVTEDEIKQFISKQQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLA 540
           PVAFVV+ K+SEVTEDEIKQFISK QVVFYKRI R F IDAIPKSPSGKILRKELRAKLA
Sbjct: 481 PVAFVVRLKNSEVTEDEIKQFISK-QVVFYKRIKRAFMIDAIPKSPSGKILRKELRAKLA 540

Query: 541 DVSPN 546
              PN
Sbjct: 541 TGFPN 544

BLAST of MS016213 vs. NCBI nr
Match: XP_022983944.1 (4-coumarate--CoA ligase 1-like [Cucurbita maxima])

HSP 1 Score: 979.5 bits (2531), Expect = 1.1e-281
Identity = 492/544 (90.44%), Postives = 515/544 (94.67%), Query Frame = 0

Query: 1   MATETLPNDFIFRSKLPDIYIPKHLPLHSYCLHENAAKIGHRTCLINGVTGDSYTYADVD 60
           MA E L NDFIFRSKLPDIYIP HLPLHSYCLHEN AKIGHRTCLIN VT +S+TY DVD
Sbjct: 1   MAVEALQNDFIFRSKLPDIYIPNHLPLHSYCLHENLAKIGHRTCLINAVTAESFTYHDVD 60

Query: 61  LTARKVASGLNKLGIAQGDVLMLLLPNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ 120
           L ARKVASGLNKLGIAQGDV++LLL NSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ
Sbjct: 61  LAARKVASGLNKLGIAQGDVILLLLQNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ 120

Query: 121 AKGSKAKLIITQASYYDKVKEITEELPDVKIMTVDSPPDGCLSFSDLTQADEREIPAVDI 180
           AKGS AKLI+TQ+SYY+KVKEITEELPDVKIMTVDSPPDGCLSF+DL QADERE+P V+I
Sbjct: 121 AKGSNAKLIVTQSSYYEKVKEITEELPDVKIMTVDSPPDGCLSFADLIQADEREMPTVEI 180

Query: 181 GPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFRNDDVILCVLPLFHI 240
            PDDVVALPYSSGTTGLPKGVMLTH+GLVTSVAQQVDGENPNLY+RN+DVILCVLPLFHI
Sbjct: 181 NPDDVVALPYSSGTTGLPKGVMLTHRGLVTSVAQQVDGENPNLYYRNEDVILCVLPLFHI 240

Query: 241 YSLNSVLLCGLRAGATILILPKFEIASLLQLVQKYRVSIAPVVPPIVLAIAKSPDLDKYD 300
           YSLNSVLLCGLRAG TILI+PKFEI SLLQLVQK++VSIAP+VPPIVLAIAKSPDLDKYD
Sbjct: 241 YSLNSVLLCGLRAGTTILIMPKFEIGSLLQLVQKFKVSIAPIVPPIVLAIAKSPDLDKYD 300

Query: 301 LSSIRVLKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360
           LSSIRV+K GGAPLGKELED+VRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG
Sbjct: 301 LSSIRVIKCGGAPLGKELEDSVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360

Query: 361 ACGTVVRNAEMKIVDTENGASLPRNTPGEICIRGDQIMKGYLNNPEATVATIDADGWLHT 420
           ACGTVVRNAEMKIVDTENG SLPRNTPGEICIRGDQIMKGYLNNPEAT ATID DGWLHT
Sbjct: 361 ACGTVVRNAEMKIVDTENGCSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420

Query: 421 GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVITDAAVVPMKDEQAGEV 480
           GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHP I+DAAVVPMKDE+AGEV
Sbjct: 421 GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPAISDAAVVPMKDEEAGEV 480

Query: 481 PVAFVVKSKSSEVTEDEIKQFISKQQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLA 540
           PVAFVV+ K+SEVTEDEIKQFISK QVVFYKRI R F IDAIPKSPSGKILRKELRAKLA
Sbjct: 481 PVAFVVRLKNSEVTEDEIKQFISK-QVVFYKRIKRAFMIDAIPKSPSGKILRKELRAKLA 540

Query: 541 DVSP 545
              P
Sbjct: 541 TGFP 543

BLAST of MS016213 vs. NCBI nr
Match: XP_038904387.1 (4-coumarate--CoA ligase 1-like [Benincasa hispida])

HSP 1 Score: 971.8 bits (2511), Expect = 2.4e-279
Identity = 488/545 (89.54%), Postives = 515/545 (94.50%), Query Frame = 0

Query: 1   MATETLPNDFIFRSKLPDIYIPKHLPLHSYCLHENAAKIGHRTCLINGVTGDSYTYADVD 60
           M  ET+ ND IFRSKLPDIYIPKHLPLHSYCLHENAAKIG RTCLINGVTG+S+TY DVD
Sbjct: 1   MGIETVQNDVIFRSKLPDIYIPKHLPLHSYCLHENAAKIGDRTCLINGVTGESFTYNDVD 60

Query: 61  LTARKVASGLNKLGIAQGDVLMLLLPNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ 120
           L+ARKVA+GLNKLGI + DV+MLLLPNSPEFVF FLGASY GAIMTAANPFFTA EIAKQ
Sbjct: 61  LSARKVAAGLNKLGITKSDVIMLLLPNSPEFVFTFLGASYLGAIMTAANPFFTAQEIAKQ 120

Query: 121 AKGSKAKLIITQASYYDKVKEITEELPDVKIMTVDSPPDGCLSFSDLTQADEREIPAVDI 180
           AKGSKAKLI+T +SYY+KVKEITEELPDVKIMTVDSPPDGCL F DL +ADER+IP V+I
Sbjct: 121 AKGSKAKLILTHSSYYEKVKEITEELPDVKIMTVDSPPDGCLWFGDLIKADERDIPTVEI 180

Query: 181 GPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFRNDDVILCVLPLFHI 240
            PDDVVALPYSSGTTGLPKGVMLTHK LVTSVAQQVDGENPNLY+RN+DVILCVLPLFHI
Sbjct: 181 DPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYRNEDVILCVLPLFHI 240

Query: 241 YSLNSVLLCGLRAGATILILPKFEIASLLQLVQKYRVSIAPVVPPIVLAIAKSPDLDKYD 300
           YSLNSVLLCGLRAG TILI+PKFEI  LLQLV+KYRVSIAP+VPPIVLAIAKSP+L+KYD
Sbjct: 241 YSLNSVLLCGLRAGTTILIMPKFEIGLLLQLVEKYRVSIAPIVPPIVLAIAKSPELEKYD 300

Query: 301 LSSIRVLKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360
           LSSIRV+K GGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG
Sbjct: 301 LSSIRVIKCGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360

Query: 361 ACGTVVRNAEMKIVDTENGASLPRNTPGEICIRGDQIMKGYLNNPEATVATIDADGWLHT 420
           ACGTVVRNAEMKIVDTE G+SLPRNTPGEICIRGDQIMKGYLNNPEAT ATID DGWLHT
Sbjct: 361 ACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420

Query: 421 GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVITDAAVVPMKDEQAGEV 480
           GDIGFIDD+DELFIVDRLKELIKYKGFQVAPAELEALLLTHPVI+DAAVVPMKDEQAGEV
Sbjct: 421 GDIGFIDDEDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV 480

Query: 481 PVAFVVKSKSSEVTEDEIKQFISKQQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLA 540
           PVAFVVK K+SEVTEDEIKQF+SK QVVFYKRINRVFFIDAIPKSPSGKILRK+LRAKLA
Sbjct: 481 PVAFVVKLKNSEVTEDEIKQFVSK-QVVFYKRINRVFFIDAIPKSPSGKILRKDLRAKLA 540

Query: 541 DVSPN 546
              PN
Sbjct: 541 AAFPN 544

BLAST of MS016213 vs. ExPASy Swiss-Prot
Match: O24145 (4-coumarate--CoA ligase 1 OS=Nicotiana tabacum OX=4097 GN=4CL1 PE=2 SV=1)

HSP 1 Score: 811.2 bits (2094), Expect = 7.0e-234
Identity = 409/544 (75.18%), Postives = 458/544 (84.19%), Query Frame = 0

Query: 3   TETLPN-DFIFRSKLPDIYIPKHLPLHSYCLHENAAKIGHRTCLINGVTGDSYTYADVDL 62
           TET  + D IFRSKLPDIYIPKHLPLHSYC  EN ++   R CLING     YTYA+V+L
Sbjct: 7   TETKQSGDLIFRSKLPDIYIPKHLPLHSYCF-ENISEFSSRPCLINGANDQIYTYAEVEL 66

Query: 63  TARKVASGLNKLGIAQGDVLMLLLPNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQA 122
           T RKVA GLNKLGI Q D +M+LLPNSPEFVFAF+GASY GAI T ANP FT AE+ KQA
Sbjct: 67  TCRKVAVGLNKLGIQQKDTIMILLPNSPEFVFAFMGASYLGAISTMANPLFTPAEVVKQA 126

Query: 123 KGSKAKLIITQASYYDKVKEITEELPDVKIMTVDSPPDGCLSFSDLTQADEREIPAVDIG 182
           K S AK+IITQ+ +  KVK+   E  DVK++ +DS P+GCL FS+LTQ+DE EIP V I 
Sbjct: 127 KASSAKIIITQSCFVGKVKDYASE-NDVKVICIDSAPEGCLHFSELTQSDEHEIPEVKIQ 186

Query: 183 PDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFRNDDVILCVLPLFHIY 242
           PDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGEN NLY  ++DV++CVLPLFHIY
Sbjct: 187 PDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSEDVLMCVLPLFHIY 246

Query: 243 SLNSVLLCGLRAGATILILPKFEIASLLQLVQKYRVSIAPVVPPIVLAIAKSPDLDKYDL 302
           SLNS+LLCGLR GA ILI+ KF+IA  L+L+QKY+VSI P VPPIVLAIAKSP +D YDL
Sbjct: 247 SLNSILLCGLRVGAAILIMQKFDIAPFLELIQKYKVSIGPFVPPIVLAIAKSPIVDSYDL 306

Query: 303 SSIRVLKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPGA 362
           SS+R + SG APLGKELED VR KFP A LGQGYGMTEAGPVL M LAFAKEPF +K GA
Sbjct: 307 SSVRTVMSGAAPLGKELEDAVRTKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSGA 366

Query: 363 CGTVVRNAEMKIVDTENGASLPRNTPGEICIRGDQIMKGYLNNPEATVATIDADGWLHTG 422
           CGTVVRNAEMKIVD + G SLPRN PGEICIRGDQIMKGYLN+PEAT  TID +GWLHTG
Sbjct: 367 CGTVVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATTRTIDKEGWLHTG 426

Query: 423 DIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVITDAAVVPMKDEQAGEVP 482
           DIGFID+DDELFIVDRLKELIKYKGFQVAPAE+EALLL HP I+DAAVVPMKDEQAGEVP
Sbjct: 427 DIGFIDEDDELFIVDRLKELIKYKGFQVAPAEIEALLLNHPNISDAAVVPMKDEQAGEVP 486

Query: 483 VAFVVKSKSSEVTEDEIKQFISKQQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLAD 542
           VAFVV+S  S +TEDE+K FISK QV+FYKR+ RVFF++ +PKSPSGKILRK+LRA+LA 
Sbjct: 487 VAFVVRSNGSAITEDEVKDFISK-QVIFYKRVKRVFFVETVPKSPSGKILRKDLRARLAA 546

Query: 543 VSPN 546
             PN
Sbjct: 547 GVPN 547

BLAST of MS016213 vs. ExPASy Swiss-Prot
Match: O24146 (4-coumarate--CoA ligase 2 OS=Nicotiana tabacum OX=4097 GN=4CL2 PE=1 SV=1)

HSP 1 Score: 807.4 bits (2084), Expect = 1.0e-232
Identity = 411/545 (75.41%), Postives = 459/545 (84.22%), Query Frame = 0

Query: 1   MATETLPNDFIFRSKLPDIYIPKHLPLHSYCLHENAAKIGHRTCLINGVTGDSYTYADVD 60
           M  +T   D IFRSKLPDIYIP HLPLHSYC  EN ++   R CLING     YTYADV+
Sbjct: 1   MEKDTKQVDIIFRSKLPDIYIPNHLPLHSYCF-ENISEFSSRPCLINGANKQIYTYADVE 60

Query: 61  LTARKVASGLNKLGIAQGDVLMLLLPNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ 120
           L +RKVA+GL+K GI   D +M+LLPNSPEFVFAF+GASY GAI T ANP FT AE+ KQ
Sbjct: 61  LNSRKVAAGLHKQGIQPKDTIMILLPNSPEFVFAFIGASYLGAISTMANPLFTPAEVVKQ 120

Query: 121 AKGSKAKLIITQASYYDKVKEITEELPDVKIMTVDSPPDGCLSFSDLTQADEREIPAVDI 180
           AK S AK+I+TQA + +KVK+   E  DVKI+ +DS P+GCL FS LTQA+E +IP V+I
Sbjct: 121 AKASSAKIIVTQACHVNKVKDYAFE-NDVKIICIDSAPEGCLHFSVLTQANEHDIPEVEI 180

Query: 181 GPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFRNDDVILCVLPLFHI 240
            PDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLY  ++DV+LCVLPLFHI
Sbjct: 181 QPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYIHSEDVMLCVLPLFHI 240

Query: 241 YSLNSVLLCGLRAGATILILPKFEIASLLQLVQKYRVSIAPVVPPIVLAIAKSPDLDKYD 300
           YSLNSVLLCGLR GA ILI+ KF+I S L+L+Q+Y+V+I P VPPIVLAIAKSP +D YD
Sbjct: 241 YSLNSVLLCGLRVGAAILIMQKFDIVSFLELIQRYKVTIGPFVPPIVLAIAKSPMVDDYD 300

Query: 301 LSSIRVLKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360
           LSS+R + SG APLGKELEDTVRAKFP A LGQGYGMTEAGPVL M LAFAKEPF +K G
Sbjct: 301 LSSVRTVMSGAAPLGKELEDTVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFEIKSG 360

Query: 361 ACGTVVRNAEMKIVDTENGASLPRNTPGEICIRGDQIMKGYLNNPEATVATIDADGWLHT 420
           ACGTVVRNAEMKIVD + G SLPRN  GEICIRGDQIMKGYLN+PEAT  TID +GWL+T
Sbjct: 361 ACGTVVRNAEMKIVDPKTGNSLPRNQSGEICIRGDQIMKGYLNDPEATARTIDKEGWLYT 420

Query: 421 GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVITDAAVVPMKDEQAGEV 480
           GDIG+IDDDDELFIVDRLKELIKYKGFQVAPAELEALLL HP I+DAAVVPMKDEQAGEV
Sbjct: 421 GDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLNHPNISDAAVVPMKDEQAGEV 480

Query: 481 PVAFVVKSKSSEVTEDEIKQFISKQQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLA 540
           PVAFVV+S  S +TEDE+K FISK QV+FYKRI RVFF+DAIPKSPSGKILRK+LRAKLA
Sbjct: 481 PVAFVVRSNGSTITEDEVKDFISK-QVIFYKRIKRVFFVDAIPKSPSGKILRKDLRAKLA 540

Query: 541 DVSPN 546
              PN
Sbjct: 541 AGLPN 542

BLAST of MS016213 vs. ExPASy Swiss-Prot
Match: I3PB37 (4-coumarate:CoA ligase 1 OS=Petunia hybrida OX=4102 GN=4CL1 PE=1 SV=1)

HSP 1 Score: 803.9 bits (2075), Expect = 1.1e-231
Identity = 403/545 (73.94%), Postives = 457/545 (83.85%), Query Frame = 0

Query: 1   MATETLPNDFIFRSKLPDIYIPKHLPLHSYCLHENAAKIGHRTCLINGVTGDSYTYADVD 60
           M TET   D IFRSKLPDIYIPKHLPLHSYC  EN ++   R CLING     YTYADV+
Sbjct: 3   METETNQGDLIFRSKLPDIYIPKHLPLHSYCF-ENISEFSSRPCLINGANNHIYTYADVE 62

Query: 61  LTARKVASGLNKLGIAQGDVLMLLLPNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ 120
           LT+RKVA+GLNKLGI Q D +M+LLPNSPEFVFAF+GASY GAI T ANP FT AE+ KQ
Sbjct: 63  LTSRKVAAGLNKLGIQQKDTIMILLPNSPEFVFAFMGASYLGAISTMANPLFTPAEVVKQ 122

Query: 121 AKGSKAKLIITQASYYDKVKEITEELPDVKIMTVDSPPDGCLSFSDLTQADEREIPAVDI 180
           AK S AKLIITQA + +KVK+   +  ++ ++ +DS P+GC+ FS+LTQADE +IP V I
Sbjct: 123 AKASNAKLIITQACFVNKVKDYAFD-NNLNVICIDSAPEGCIHFSELTQADEHDIPDVKI 182

Query: 181 GPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFRNDDVILCVLPLFHI 240
             DDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGEN NLY  ++DV++CVLPLFHI
Sbjct: 183 QSDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSEDVLMCVLPLFHI 242

Query: 241 YSLNSVLLCGLRAGATILILPKFEIASLLQLVQKYRVSIAPVVPPIVLAIAKSPDLDKYD 300
           YSLNSVLLCGLR GA ILI+ KF+I    +L++KY+V+I P VPPIVLAIAKSP +D YD
Sbjct: 243 YSLNSVLLCGLRVGAAILIMQKFDIVQFCELIEKYKVTIGPFVPPIVLAIAKSPVVDNYD 302

Query: 301 LSSIRVLKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360
           LSS+R + SG APLGKELED VR KFP A LGQGYGMTEAGPVL M LAFAKEPF +K G
Sbjct: 303 LSSVRTVMSGAAPLGKELEDAVRIKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSG 362

Query: 361 ACGTVVRNAEMKIVDTENGASLPRNTPGEICIRGDQIMKGYLNNPEATVATIDADGWLHT 420
           ACGTVVRNAEMKIVD + G SLPRN PGEICIRGDQIMKGYLN+P AT  TID +GWLHT
Sbjct: 363 ACGTVVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPAATTRTIDKEGWLHT 422

Query: 421 GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVITDAAVVPMKDEQAGEV 480
           GDIG+ID+DDELFIVDRLKELIKYKGFQVAPAELEALLL HP I+DAAVVPMKDEQAGEV
Sbjct: 423 GDIGYIDNDDELFIVDRLKELIKYKGFQVAPAELEALLLNHPNISDAAVVPMKDEQAGEV 482

Query: 481 PVAFVVKSKSSEVTEDEIKQFISKQQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLA 540
           PVAFVV+S  S++TEDE+K F+SK QV+FYKRI RVFF++ +PKSPSGKILRK+LRA+LA
Sbjct: 483 PVAFVVRSNGSDITEDEVKDFVSK-QVIFYKRIKRVFFVETVPKSPSGKILRKDLRARLA 542

Query: 541 DVSPN 546
              PN
Sbjct: 543 AGVPN 544

BLAST of MS016213 vs. ExPASy Swiss-Prot
Match: M4ISH0 (4-coumarate--CoA ligase CCL1 OS=Humulus lupulus OX=3486 GN=CCL1 PE=1 SV=1)

HSP 1 Score: 803.9 bits (2075), Expect = 1.1e-231
Identity = 407/533 (76.36%), Postives = 459/533 (86.12%), Query Frame = 0

Query: 9   DFIFRSKLPDIYIPKHLPLHSYCLHENAAKIGHRTCLINGVTGDSYTYADVDLTARKVAS 68
           +FIFRSKLPDIYIP HLPLHSYC  EN ++   R CLING TG+  TYADVDLT+RKVA+
Sbjct: 12  EFIFRSKLPDIYIPNHLPLHSYCF-ENISQFKDRPCLINGATGEIITYADVDLTSRKVAA 71

Query: 69  GLNKLGIAQGDVLMLLLPNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQAKGSKAKL 128
           GL+KLGI QGDV+MLLL NSPEFV+AFL ASY GAI+T ANPF+T AE+AKQA  SK KL
Sbjct: 72  GLDKLGIKQGDVIMLLLQNSPEFVYAFLAASYIGAIITTANPFYTPAEVAKQAAASKTKL 131

Query: 129 IITQASYYDKVKEITEELPDVKIMTVDSPP--DGCLSFSDLTQADEREIPAVDIGPDDVV 188
           +IT A Y DKVKE T     VK+M VD+PP    CL FS+LTQADE EIPAV I PDDVV
Sbjct: 132 VITLAGYIDKVKEFTGGESGVKVMCVDAPPPESECLHFSELTQADETEIPAVKIHPDDVV 191

Query: 189 ALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFRNDDVILCVLPLFHIYSLNSV 248
           ALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDG+NPNLYF  +DVILCVLPLFHIYSLNS+
Sbjct: 192 ALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGDNPNLYFHQNDVILCVLPLFHIYSLNSI 251

Query: 249 LLCGLRAGATILILPKFEIASLLQLVQKYRVSIAPVVPPIVLAIAKSPDLDKYDLSSIRV 308
           LLCGLR GA ILI+ KFEI+ LL+L++K++V+IAP VPPIVL++AK PDL +YDLSSIR 
Sbjct: 252 LLCGLRVGAAILIMQKFEISKLLELIEKFKVTIAPFVPPIVLSVAKCPDLHRYDLSSIRT 311

Query: 309 LKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPGACGTVV 368
           + SGGAP+GKELED V+ K P A LGQGYGMTEAGPVL+M LAFAKEPFP+K GACGTVV
Sbjct: 312 VMSGGAPMGKELEDAVKEKLPHAKLGQGYGMTEAGPVLSMCLAFAKEPFPIKSGACGTVV 371

Query: 369 RNAEMKIVDTENGASLPRNTPGEICIRGDQIMKGYLNNPEATVATIDADGWLHTGDIGFI 428
           RNAEMKIVD + GASLPRN  GEICIRG QIMKGY+N+ EAT  TID  GWLHTGDIGFI
Sbjct: 372 RNAEMKIVDPDTGASLPRNQSGEICIRGKQIMKGYINDAEATKGTIDEGGWLHTGDIGFI 431

Query: 429 DDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVITDAAVVPMKDEQAGEVPVAFVV 488
           D+DDELFIVDRLKELIKYKGFQVAPAELE++L++HP ITDAAVVPMKDE AGEVPVAFVV
Sbjct: 432 DNDDELFIVDRLKELIKYKGFQVAPAELESMLISHPNITDAAVVPMKDEAAGEVPVAFVV 491

Query: 489 KSKSSEVTEDEIKQFISKQQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKL 540
           +S  S++TE++IKQ+ISK QVVFYKRIN+ FFI+ IPK+PSGKILRK LRAKL
Sbjct: 492 RSNGSKITEEDIKQYISK-QVVFYKRINKAFFIEEIPKNPSGKILRKILRAKL 542

BLAST of MS016213 vs. ExPASy Swiss-Prot
Match: P14912 (4-coumarate--CoA ligase 1 OS=Petroselinum crispum OX=4043 GN=4CL1 PE=2 SV=1)

HSP 1 Score: 802.7 bits (2072), Expect = 2.5e-231
Identity = 402/532 (75.56%), Postives = 458/532 (86.09%), Query Frame = 0

Query: 9   DFIFRSKLPDIYIPKHLPLHSYCLHENAAKIGHRTCLINGVTGDSYTYADVDLTARKVAS 68
           D IFRSKLPDIYIPKHLPLH+YC  EN +K+G ++CLING TG+++TY+ V+L +RKVAS
Sbjct: 10  DLIFRSKLPDIYIPKHLPLHTYCF-ENISKVGDKSCLINGATGETFTYSQVELLSRKVAS 69

Query: 69  GLNKLGIAQGDVLMLLLPNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQAKGSKAKL 128
           GLNKLGI QGD +MLLLPNSPE+ FAFLGASYRGAI T ANPFFT+AE+ KQ K S+AKL
Sbjct: 70  GLNKLGIQQGDTIMLLLPNSPEYFFAFLGASYRGAISTMANPFFTSAEVIKQLKASQAKL 129

Query: 129 IITQASYYDKVKEITEELPDVKIMTVDSPPDGCLSFSDLTQADEREIPAVDIGPDDVVAL 188
           IITQA Y DKVK+   E  +++I+ +D  P  CL FS L +ADE E+P V I  DDVVAL
Sbjct: 130 IITQACYVDKVKDYAAE-KNIQIICIDDAPQDCLHFSKLMEADESEMPEVVINSDDVVAL 189

Query: 189 PYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFRNDDVILCVLPLFHIYSLNSVLL 248
           PYSSGTTGLPKGVMLTHKGLVTSVAQQVDG+NPNLY  ++DV++C+LPLFHIYSLN+VL 
Sbjct: 190 PYSSGTTGLPKGVMLTHKGLVTSVAQQVDGDNPNLYMHSEDVMICILPLFHIYSLNAVLC 249

Query: 249 CGLRAGATILILPKFEIASLLQLVQKYRVSIAPVVPPIVLAIAKSPDLDKYDLSSIRVLK 308
           CGLRAG TILI+ KF+I   L+L+QKY+V+I P VPPIVLAIAKSP +DKYDLSS+R + 
Sbjct: 250 CGLRAGVTILIMQKFDIVPFLELIQKYKVTIGPFVPPIVLAIAKSPVVDKYDLSSVRTVM 309

Query: 309 SGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPGACGTVVRN 368
           SG APLGKELED VRAKFP A LGQGYGMTEAGPVL M LAFAKEP+ +K GACGTVVRN
Sbjct: 310 SGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPYEIKSGACGTVVRN 369

Query: 369 AEMKIVDTENGASLPRNTPGEICIRGDQIMKGYLNNPEATVATIDADGWLHTGDIGFIDD 428
           AEMKIVD E  ASLPRN  GEICIRGDQIMKGYLN+PE+T  TID +GWLHTGDIGFIDD
Sbjct: 370 AEMKIVDPETNASLPRNQRGEICIRGDQIMKGYLNDPESTRTTIDEEGWLHTGDIGFIDD 429

Query: 429 DDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVITDAAVVPMKDEQAGEVPVAFVVKS 488
           DDELFIVDRLKE+IKYKGFQVAPAELEALLLTHP I+DAAVVPM DE+AGEVPVAFVV++
Sbjct: 430 DDELFIVDRLKEIIKYKGFQVAPAELEALLLTHPTISDAAVVPMIDEKAGEVPVAFVVRT 489

Query: 489 KSSEVTEDEIKQFISKQQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLA 541
                TE+EIKQF+SK QVVFYKRI RVFF+DAIPKSPSGKILRK+LRA++A
Sbjct: 490 NGFTTTEEEIKQFVSK-QVVFYKRIFRVFFVDAIPKSPSGKILRKDLRARIA 538

BLAST of MS016213 vs. ExPASy TrEMBL
Match: A0A6J1F599 (4-coumarate--CoA ligase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111442288 PE=4 SV=1)

HSP 1 Score: 984.6 bits (2544), Expect = 1.7e-283
Identity = 494/545 (90.64%), Postives = 517/545 (94.86%), Query Frame = 0

Query: 1   MATETLPNDFIFRSKLPDIYIPKHLPLHSYCLHENAAKIGHRTCLINGVTGDSYTYADVD 60
           MA E L NDFIFRSKLPDIYIP HLPLHSYCLHEN AKIGHRTCLIN VTG+S+TY DVD
Sbjct: 1   MAVEALQNDFIFRSKLPDIYIPNHLPLHSYCLHENLAKIGHRTCLINSVTGESFTYHDVD 60

Query: 61  LTARKVASGLNKLGIAQGDVLMLLLPNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ 120
           L ARKVASGLNKLGIAQGDV+ML+L NSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ
Sbjct: 61  LAARKVASGLNKLGIAQGDVIMLILQNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ 120

Query: 121 AKGSKAKLIITQASYYDKVKEITEELPDVKIMTVDSPPDGCLSFSDLTQADEREIPAVDI 180
           AKGS AKLI+TQ+SYY+KVKEITEELPDVKIMTVDSPPDGCLSF+DL QADERE+P V+I
Sbjct: 121 AKGSNAKLIVTQSSYYEKVKEITEELPDVKIMTVDSPPDGCLSFADLIQADEREMPTVEI 180

Query: 181 GPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFRNDDVILCVLPLFHI 240
            PDDVVALPYSSGTTGLPKGVMLTH+GLVTSVAQQVDGENPNLY+RN+DVILCVLPLFHI
Sbjct: 181 NPDDVVALPYSSGTTGLPKGVMLTHRGLVTSVAQQVDGENPNLYYRNEDVILCVLPLFHI 240

Query: 241 YSLNSVLLCGLRAGATILILPKFEIASLLQLVQKYRVSIAPVVPPIVLAIAKSPDLDKYD 300
           YSLNSVLLCGLRAG TILI+PKFEI SLLQLVQK++VSIAP+VPPIVLAIAKSPDLDKYD
Sbjct: 241 YSLNSVLLCGLRAGTTILIMPKFEIGSLLQLVQKFKVSIAPIVPPIVLAIAKSPDLDKYD 300

Query: 301 LSSIRVLKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360
           LSSIRV+K GGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG
Sbjct: 301 LSSIRVIKCGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360

Query: 361 ACGTVVRNAEMKIVDTENGASLPRNTPGEICIRGDQIMKGYLNNPEATVATIDADGWLHT 420
           ACGTVVRNAEMKIVDTENG SLPRNTPGEICIRGDQIMKGYLNNPEAT ATID DGWLHT
Sbjct: 361 ACGTVVRNAEMKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420

Query: 421 GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVITDAAVVPMKDEQAGEV 480
           GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAE+EALLLTHP I+DAAVVPMKDE+AGEV
Sbjct: 421 GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAEVEALLLTHPAISDAAVVPMKDEEAGEV 480

Query: 481 PVAFVVKSKSSEVTEDEIKQFISKQQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLA 540
           PVAFVV+ K+SEVTEDEIKQFISK QVVFYKRI R F IDAIPKSPSGKILRKELRAKLA
Sbjct: 481 PVAFVVRLKNSEVTEDEIKQFISK-QVVFYKRIKRAFMIDAIPKSPSGKILRKELRAKLA 540

Query: 541 DVSPN 546
              PN
Sbjct: 541 AGFPN 544

BLAST of MS016213 vs. ExPASy TrEMBL
Match: A0A6J1J912 (4-coumarate--CoA ligase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111482411 PE=4 SV=1)

HSP 1 Score: 979.5 bits (2531), Expect = 5.5e-282
Identity = 492/544 (90.44%), Postives = 515/544 (94.67%), Query Frame = 0

Query: 1   MATETLPNDFIFRSKLPDIYIPKHLPLHSYCLHENAAKIGHRTCLINGVTGDSYTYADVD 60
           MA E L NDFIFRSKLPDIYIP HLPLHSYCLHEN AKIGHRTCLIN VT +S+TY DVD
Sbjct: 1   MAVEALQNDFIFRSKLPDIYIPNHLPLHSYCLHENLAKIGHRTCLINAVTAESFTYHDVD 60

Query: 61  LTARKVASGLNKLGIAQGDVLMLLLPNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ 120
           L ARKVASGLNKLGIAQGDV++LLL NSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ
Sbjct: 61  LAARKVASGLNKLGIAQGDVILLLLQNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ 120

Query: 121 AKGSKAKLIITQASYYDKVKEITEELPDVKIMTVDSPPDGCLSFSDLTQADEREIPAVDI 180
           AKGS AKLI+TQ+SYY+KVKEITEELPDVKIMTVDSPPDGCLSF+DL QADERE+P V+I
Sbjct: 121 AKGSNAKLIVTQSSYYEKVKEITEELPDVKIMTVDSPPDGCLSFADLIQADEREMPTVEI 180

Query: 181 GPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFRNDDVILCVLPLFHI 240
            PDDVVALPYSSGTTGLPKGVMLTH+GLVTSVAQQVDGENPNLY+RN+DVILCVLPLFHI
Sbjct: 181 NPDDVVALPYSSGTTGLPKGVMLTHRGLVTSVAQQVDGENPNLYYRNEDVILCVLPLFHI 240

Query: 241 YSLNSVLLCGLRAGATILILPKFEIASLLQLVQKYRVSIAPVVPPIVLAIAKSPDLDKYD 300
           YSLNSVLLCGLRAG TILI+PKFEI SLLQLVQK++VSIAP+VPPIVLAIAKSPDLDKYD
Sbjct: 241 YSLNSVLLCGLRAGTTILIMPKFEIGSLLQLVQKFKVSIAPIVPPIVLAIAKSPDLDKYD 300

Query: 301 LSSIRVLKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360
           LSSIRV+K GGAPLGKELED+VRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG
Sbjct: 301 LSSIRVIKCGGAPLGKELEDSVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360

Query: 361 ACGTVVRNAEMKIVDTENGASLPRNTPGEICIRGDQIMKGYLNNPEATVATIDADGWLHT 420
           ACGTVVRNAEMKIVDTENG SLPRNTPGEICIRGDQIMKGYLNNPEAT ATID DGWLHT
Sbjct: 361 ACGTVVRNAEMKIVDTENGCSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420

Query: 421 GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVITDAAVVPMKDEQAGEV 480
           GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHP I+DAAVVPMKDE+AGEV
Sbjct: 421 GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPAISDAAVVPMKDEEAGEV 480

Query: 481 PVAFVVKSKSSEVTEDEIKQFISKQQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLA 540
           PVAFVV+ K+SEVTEDEIKQFISK QVVFYKRI R F IDAIPKSPSGKILRKELRAKLA
Sbjct: 481 PVAFVVRLKNSEVTEDEIKQFISK-QVVFYKRIKRAFMIDAIPKSPSGKILRKELRAKLA 540

Query: 541 DVSP 545
              P
Sbjct: 541 TGFP 543

BLAST of MS016213 vs. ExPASy TrEMBL
Match: A0A6J1G9T7 (4-coumarate--CoA ligase 1-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111452267 PE=4 SV=1)

HSP 1 Score: 964.9 bits (2493), Expect = 1.4e-277
Identity = 489/540 (90.56%), Postives = 516/540 (95.56%), Query Frame = 0

Query: 1   MATETLPNDFIFRSKLPDIYIPKHLPLHSYCLHENAAKIGHRTCLINGVTGDSYTYADVD 60
           MATET+ +DFIFRSKLPDIYIPKHLPLHSYCL E  A+IGHRTCLIN VTG+S+TY+DVD
Sbjct: 1   MATETV-HDFIFRSKLPDIYIPKHLPLHSYCLQEKMAEIGHRTCLINAVTGESFTYSDVD 60

Query: 61  LTARKVASGLNKLGIAQGDVLMLLLPNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ 120
           L ARK ASGL KLGIA+GDV+MLLLPNSPEFVFAFLGASY GAIMTAANPFFTAAEIAKQ
Sbjct: 61  LAARKAASGLYKLGIAKGDVIMLLLPNSPEFVFAFLGASYLGAIMTAANPFFTAAEIAKQ 120

Query: 121 AKGSKAKLIITQASYYDKVKEITEELPDVKIMTVDSPPDGCLSFSDLTQADEREIPAVDI 180
           AKGSKAKLIITQ+SYY+KVKEITE+L D KIMTVDSP  GCLSF+DL Q  + +IPAV+I
Sbjct: 121 AKGSKAKLIITQSSYYEKVKEITEQLSDAKIMTVDSPSLGCLSFADLIQGGDHKIPAVEI 180

Query: 181 GPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFRNDDVILCVLPLFHI 240
            PDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLY+RNDDVILCVLPLFHI
Sbjct: 181 SPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYYRNDDVILCVLPLFHI 240

Query: 241 YSLNSVLLCGLRAGATILILPKFEIASLLQLVQKYRVSIAPVVPPIVLAIAKSPDLDKYD 300
           YSLNSVLLCGLRAGATILI+PKFEI SLLQLV+KYRVSIAP+VPPIVLAIAKSPDL+KYD
Sbjct: 241 YSLNSVLLCGLRAGATILIMPKFEIGSLLQLVEKYRVSIAPIVPPIVLAIAKSPDLEKYD 300

Query: 301 LSSIRVLKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360
           LSSIR++KSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG
Sbjct: 301 LSSIRMIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360

Query: 361 ACGTVVRNAEMKIVDTENGASLPRNTPGEICIRGDQIMKGYLNNPEATVATIDADGWLHT 420
           ACGTVVRNAE+KIVDTENG SLPRNTPGEICIRGDQIMKGYLNNPEAT ATID++GWLHT
Sbjct: 361 ACGTVVRNAEIKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDSEGWLHT 420

Query: 421 GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVITDAAVVPMKDEQAGEV 480
           GDIG IDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVI+DAAVVPMKDEQAGEV
Sbjct: 421 GDIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV 480

Query: 481 PVAFVVKSKSSEVTEDEIKQFISKQQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLA 540
           PVAFVVK K+SEVTEDEIKQFISK QVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLA
Sbjct: 481 PVAFVVKLKNSEVTEDEIKQFISK-QVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLA 538

BLAST of MS016213 vs. ExPASy TrEMBL
Match: A0A5A7SWF8 (4-coumarate--CoA ligase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1199G00110 PE=4 SV=1)

HSP 1 Score: 963.8 bits (2490), Expect = 3.1e-277
Identity = 486/545 (89.17%), Postives = 515/545 (94.50%), Query Frame = 0

Query: 1   MATETLPNDFIFRSKLPDIYIPKHLPLHSYCLHENAAKIGHRTCLINGVTGDSYTYADVD 60
           M  ET+ ND IFRSKLPDIYIP HLPLHSYCL ENAAKIGHRTCLINGVTG+S+T+ DVD
Sbjct: 1   MGIETVENDVIFRSKLPDIYIPNHLPLHSYCLQENAAKIGHRTCLINGVTGESFTFNDVD 60

Query: 61  LTARKVASGLNKLGIAQGDVLMLLLPNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ 120
           LTARKVASGLNKLGI + DV+MLLLPNSPEFVFAFLGAS+ GAIMTAANPFFTAAEIAKQ
Sbjct: 61  LTARKVASGLNKLGITKRDVIMLLLPNSPEFVFAFLGASHLGAIMTAANPFFTAAEIAKQ 120

Query: 121 AKGSKAKLIITQASYYDKVKEITEELPDVKIMTVDSPPDGCLSFSDLTQADEREIPAVDI 180
           AKGSKAKLIITQ+SYY+K+KEITEELP+VKIMTVDSP DGCL F DL +ADEREIP V+I
Sbjct: 121 AKGSKAKLIITQSSYYEKLKEITEELPEVKIMTVDSPHDGCLWFGDLIKADEREIPRVEI 180

Query: 181 GPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFRNDDVILCVLPLFHI 240
            PDDVVALPYSSGTTGLPKGVMLTHK LVTSVAQQVDGENPNLY+ N+DVILCVLPLFHI
Sbjct: 181 DPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGNEDVILCVLPLFHI 240

Query: 241 YSLNSVLLCGLRAGATILILPKFEIASLLQLVQKYRVSIAPVVPPIVLAIAKSPDLDKYD 300
           YSLNSVLLCGLRAG+TILI+PKFEI  LLQLV+KY V++AP+VPPIVLAIAKSP+L+KYD
Sbjct: 241 YSLNSVLLCGLRAGSTILIMPKFEIGLLLQLVEKYGVTVAPIVPPIVLAIAKSPELEKYD 300

Query: 301 LSSIRVLKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360
           LSSIRV+KSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG
Sbjct: 301 LSSIRVIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360

Query: 361 ACGTVVRNAEMKIVDTENGASLPRNTPGEICIRGDQIMKGYLNNPEATVATIDADGWLHT 420
           ACGTVVRNAEMKIVDTE G+SLPRNTPGEICIRGDQIMKGYLNNPEAT ATID DGWLHT
Sbjct: 361 ACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420

Query: 421 GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVITDAAVVPMKDEQAGEV 480
           GDIGFID+DDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVI+DAAVVPMKDEQAGEV
Sbjct: 421 GDIGFIDEDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV 480

Query: 481 PVAFVVKSKSSEVTEDEIKQFISKQQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLA 540
           PVAFVVK K+SE TEDEIKQFISK QVVFYK+INRVFFIDAIPKSPSGKILRKELRAKLA
Sbjct: 481 PVAFVVKLKNSEATEDEIKQFISK-QVVFYKKINRVFFIDAIPKSPSGKILRKELRAKLA 540

Query: 541 DVSPN 546
              PN
Sbjct: 541 AAFPN 544

BLAST of MS016213 vs. ExPASy TrEMBL
Match: A0A6J1KCG9 (4-coumarate--CoA ligase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111493060 PE=4 SV=1)

HSP 1 Score: 959.9 bits (2480), Expect = 4.5e-276
Identity = 486/540 (90.00%), Postives = 513/540 (95.00%), Query Frame = 0

Query: 1   MATETLPNDFIFRSKLPDIYIPKHLPLHSYCLHENAAKIGHRTCLINGVTGDSYTYADVD 60
           MA ET+ +D IFRSKLPDIYIPKHLPLHSYCL E  A+IGHRTCLIN VTG+S+TY+DVD
Sbjct: 1   MANETV-HDIIFRSKLPDIYIPKHLPLHSYCLQEKMAEIGHRTCLINAVTGESFTYSDVD 60

Query: 61  LTARKVASGLNKLGIAQGDVLMLLLPNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ 120
           L ARK ASGL KLGIA+GDV+MLLLPNSPEFVFAFLGASY GAIMTAANPFFTAAEIAKQ
Sbjct: 61  LAARKAASGLYKLGIAKGDVIMLLLPNSPEFVFAFLGASYLGAIMTAANPFFTAAEIAKQ 120

Query: 121 AKGSKAKLIITQASYYDKVKEITEELPDVKIMTVDSPPDGCLSFSDLTQADEREIPAVDI 180
           AKGSKAKL ITQ+SYY+KVKEITE+LPD KIMTVDSPP GCLSF+DL Q  + EIPAV+I
Sbjct: 121 AKGSKAKLTITQSSYYEKVKEITEQLPDAKIMTVDSPPLGCLSFADLIQGGDHEIPAVEI 180

Query: 181 GPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFRNDDVILCVLPLFHI 240
            PDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLY+RNDDVILCVLPLFHI
Sbjct: 181 SPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYYRNDDVILCVLPLFHI 240

Query: 241 YSLNSVLLCGLRAGATILILPKFEIASLLQLVQKYRVSIAPVVPPIVLAIAKSPDLDKYD 300
           YSLNSVLLCGLRAGATILI+PKFEI SLLQLV+KYRVSIAP+VPPIVLAIAKSPDL+KYD
Sbjct: 241 YSLNSVLLCGLRAGATILIMPKFEIGSLLQLVEKYRVSIAPIVPPIVLAIAKSPDLEKYD 300

Query: 301 LSSIRVLKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360
           LSSIR++KSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG
Sbjct: 301 LSSIRMIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360

Query: 361 ACGTVVRNAEMKIVDTENGASLPRNTPGEICIRGDQIMKGYLNNPEATVATIDADGWLHT 420
           ACGTVVRNAE+KIVDTENG SLPRNTPGEICI+GDQIMKGYLNNPEAT ATID +GWLHT
Sbjct: 361 ACGTVVRNAEIKIVDTENGGSLPRNTPGEICIKGDQIMKGYLNNPEATAATIDNEGWLHT 420

Query: 421 GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVITDAAVVPMKDEQAGEV 480
           GDIG IDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVI+DAAVVPMKDEQAGEV
Sbjct: 421 GDIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV 480

Query: 481 PVAFVVKSKSSEVTEDEIKQFISKQQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLA 540
           PVAFVVK K+S+VTEDEIKQFISK QVVFYKRINRVFFI AIPKSPSGKILRKELRAKLA
Sbjct: 481 PVAFVVKLKNSKVTEDEIKQFISK-QVVFYKRINRVFFIGAIPKSPSGKILRKELRAKLA 538

BLAST of MS016213 vs. TAIR 10
Match: AT3G21240.1 (4-coumarate:CoA ligase 2 )

HSP 1 Score: 770.4 bits (1988), Expect = 9.7e-223
Identity = 386/538 (71.75%), Postives = 455/538 (84.57%), Query Frame = 0

Query: 8   NDFIFRSKLPDIYIPKHLPLHSYCLHENAAKIGHRTCLINGVTGDSYTYADVDLTARKVA 67
           ND IFRS+LPDIYIP HLPLH Y + EN ++   + CLING TG+ YTYADV +T+RK+A
Sbjct: 19  NDVIFRSRLPDIYIPNHLPLHDY-IFENISEFAAKPCLINGPTGEVYTYADVHVTSRKLA 78

Query: 68  SGLNKLGIAQGDVLMLLLPNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQAKGSKAK 127
           +GL+ LG+ Q DV+M+LLPNSPE V  FL AS+ GAI T+ANPFFT AEI+KQAK S AK
Sbjct: 79  AGLHNLGVKQHDVVMILLPNSPEVVLTFLAASFIGAITTSANPFFTPAEISKQAKASAAK 138

Query: 128 LIITQASYYDKVKEITEELPDVKIMTVDSP--PDGCLSFSDLTQADEREIPAV--DIGPD 187
           LI+TQ+ Y DK+K +  +   V I+T DS   P+ CL FS+LTQ++E  + ++   I P+
Sbjct: 139 LIVTQSRYVDKIKNLQND--GVLIVTTDSDAIPENCLRFSELTQSEEPRVDSIPEKISPE 198

Query: 188 DVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFRNDDVILCVLPLFHIYSL 247
           DVVALP+SSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYF  DDVILCVLP+FHIY+L
Sbjct: 199 DVVALPFSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFNRDDVILCVLPMFHIYAL 258

Query: 248 NSVLLCGLRAGATILILPKFEIASLLQLVQKYRVSIAPVVPPIVLAIAKSPDLDKYDLSS 307
           NS++LC LR GATILI+PKFEI  LL+ +Q+ +V++A VVPPIVLAIAKSP+ +KYDLSS
Sbjct: 259 NSIMLCSLRVGATILIMPKFEITLLLEQIQRCKVTVAMVVPPIVLAIAKSPETEKYDLSS 318

Query: 308 IRVLKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPGACG 367
           +R++KSG APLGKELED + AKFP A LGQGYGMTEAGPVL M L FAKEPFP+K GACG
Sbjct: 319 VRMVKSGAAPLGKELEDAISAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPVKSGACG 378

Query: 368 TVVRNAEMKIVDTENGASLPRNTPGEICIRGDQIMKGYLNNPEATVATIDADGWLHTGDI 427
           TVVRNAEMKI+D + G SLPRN PGEICIRG+QIMKGYLN+P AT +TID DGWLHTGD+
Sbjct: 379 TVVRNAEMKILDPDTGDSLPRNKPGEICIRGNQIMKGYLNDPLATASTIDKDGWLHTGDV 438

Query: 428 GFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVITDAAVVPMKDEQAGEVPVA 487
           GFIDDDDELFIVDRLKELIKYKGFQVAPAELE+LL+ HP I D AVV MK+E AGEVPVA
Sbjct: 439 GFIDDDDELFIVDRLKELIKYKGFQVAPAELESLLIGHPEINDVAVVAMKEEDAGEVPVA 498

Query: 488 FVVKSKSSEVTEDEIKQFISKQQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLAD 542
           FVV+SK S ++EDEIKQF+SK QVVFYKRIN+VFF D+IPK+PSGKILRK+LRA+LA+
Sbjct: 499 FVVRSKDSNISEDEIKQFVSK-QVVFYKRINKVFFTDSIPKAPSGKILRKDLRARLAN 552

BLAST of MS016213 vs. TAIR 10
Match: AT1G51680.1 (4-coumarate:CoA ligase 1 )

HSP 1 Score: 765.0 bits (1974), Expect = 4.1e-221
Identity = 390/542 (71.96%), Postives = 450/542 (83.03%), Query Frame = 0

Query: 8   NDFIFRSKLPDIYIPKHLPLHSYCLHENAAKIGHRTCLINGVTGDSYTYADVDLTARKVA 67
           +D IFRSKLPDIYIP HL LH Y + +N ++   + CLING TG  YTY+DV + +R++A
Sbjct: 21  SDVIFRSKLPDIYIPNHLSLHDY-IFQNISEFATKPCLINGPTGHVYTYSDVHVISRQIA 80

Query: 68  SGLNKLGIAQGDVLMLLLPNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQAKGSKAK 127
           +  +KLG+ Q DV+MLLLPN PEFV +FL AS+RGA  TAANPFFT AEIAKQAK S  K
Sbjct: 81  ANFHKLGVNQNDVVMLLLPNCPEFVLSFLAASFRGATATAANPFFTPAEIAKQAKASNTK 140

Query: 128 LIITQASYYDKVKEITEELPDVKIMTVDSP-----PDGCLSFSDLTQAD---EREIPAVD 187
           LIIT+A Y DK+K +  +   V I+ +D       P+GCL F++LTQ+       I +V+
Sbjct: 141 LIITEARYVDKIKPLQND-DGVVIVCIDDNESVPIPEGCLRFTELTQSTTEASEVIDSVE 200

Query: 188 IGPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFRNDDVILCVLPLFH 247
           I PDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYF +DDVILCVLP+FH
Sbjct: 201 ISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHSDDVILCVLPMFH 260

Query: 248 IYSLNSVLLCGLRAGATILILPKFEIASLLQLVQKYRVSIAPVVPPIVLAIAKSPDLDKY 307
           IY+LNS++LCGLR GA ILI+PKFEI  LL+L+Q+ +V++AP+VPPIVLAIAKS + +KY
Sbjct: 261 IYALNSIMLCGLRVGAAILIMPKFEINLLLELIQRCKVTVAPMVPPIVLAIAKSSETEKY 320

Query: 308 DLSSIRVLKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKP 367
           DLSSIRV+KSG APLGKELED V AKFP A LGQGYGMTEAGPVL M L FAKEPFP+K 
Sbjct: 321 DLSSIRVVKSGAAPLGKELEDAVNAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPVKS 380

Query: 368 GACGTVVRNAEMKIVDTENGASLPRNTPGEICIRGDQIMKGYLNNPEATVATIDADGWLH 427
           GACGTVVRNAEMKIVD + G SL RN PGEICIRG QIMKGYLNNP AT  TID DGWLH
Sbjct: 381 GACGTVVRNAEMKIVDPDTGDSLSRNQPGEICIRGHQIMKGYLNNPAATAETIDKDGWLH 440

Query: 428 TGDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVITDAAVVPMKDEQAGE 487
           TGDIG IDDDDELFIVDRLKELIKYKGFQVAPAELEALL+ HP ITD AVV MK+E AGE
Sbjct: 441 TGDIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLIGHPDITDVAVVAMKEEAAGE 500

Query: 488 VPVAFVVKSKSSEVTEDEIKQFISKQQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKL 542
           VPVAFVVKSK SE++ED++KQF+SK QVVFYKRIN+VFF ++IPK+PSGKILRK+LRAKL
Sbjct: 501 VPVAFVVKSKDSELSEDDVKQFVSK-QVVFYKRINKVFFTESIPKAPSGKILRKDLRAKL 559

BLAST of MS016213 vs. TAIR 10
Match: AT1G51680.3 (4-coumarate:CoA ligase 1 )

HSP 1 Score: 712.6 bits (1838), Expect = 2.4e-205
Identity = 362/506 (71.54%), Postives = 416/506 (82.21%), Query Frame = 0

Query: 8   NDFIFRSKLPDIYIPKHLPLHSYCLHENAAKIGHRTCLINGVTGDSYTYADVDLTARKVA 67
           +D IFRSKLPDIYIP HL LH Y + +N ++   + CLING TG  YTY+DV + +R++A
Sbjct: 21  SDVIFRSKLPDIYIPNHLSLHDY-IFQNISEFATKPCLINGPTGHVYTYSDVHVISRQIA 80

Query: 68  SGLNKLGIAQGDVLMLLLPNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQAKGSKAK 127
           +  +KLG+ Q DV+MLLLPN PEFV +FL AS+RGA  TAANPFFT AEIAKQAK S  K
Sbjct: 81  ANFHKLGVNQNDVVMLLLPNCPEFVLSFLAASFRGATATAANPFFTPAEIAKQAKASNTK 140

Query: 128 LIITQASYYDKVKEITEELPDVKIMTVDSP-----PDGCLSFSDLTQAD---EREIPAVD 187
           LIIT+A Y DK+K +  +   V I+ +D       P+GCL F++LTQ+       I +V+
Sbjct: 141 LIITEARYVDKIKPLQND-DGVVIVCIDDNESVPIPEGCLRFTELTQSTTEASEVIDSVE 200

Query: 188 IGPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFRNDDVILCVLPLFH 247
           I PDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYF +DDVILCVLP+FH
Sbjct: 201 ISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHSDDVILCVLPMFH 260

Query: 248 IYSLNSVLLCGLRAGATILILPKFEIASLLQLVQKYRVSIAPVVPPIVLAIAKSPDLDKY 307
           IY+LNS++LCGLR GA ILI+PKFEI  LL+L+Q+ +V++AP+VPPIVLAIAKS + +KY
Sbjct: 261 IYALNSIMLCGLRVGAAILIMPKFEINLLLELIQRCKVTVAPMVPPIVLAIAKSSETEKY 320

Query: 308 DLSSIRVLKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKP 367
           DLSSIRV+KSG APLGKELED V AKFP A LGQGYGMTEAGPVL M L FAKEPFP+K 
Sbjct: 321 DLSSIRVVKSGAAPLGKELEDAVNAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPVKS 380

Query: 368 GACGTVVRNAEMKIVDTENGASLPRNTPGEICIRGDQIMKGYLNNPEATVATIDADGWLH 427
           GACGTVVRNAEMKIVD + G SL RN PGEICIRG QIMKGYLNNP AT  TID DGWLH
Sbjct: 381 GACGTVVRNAEMKIVDPDTGDSLSRNQPGEICIRGHQIMKGYLNNPAATAETIDKDGWLH 440

Query: 428 TGDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVITDAAVVPMKDEQAGE 487
           TGDIG IDDDDELFIVDRLKELIKYKGFQVAPAELEALL+ HP ITD AVV MK+E AGE
Sbjct: 441 TGDIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLIGHPDITDVAVVAMKEEAAGE 500

Query: 488 VPVAFVVKSKSSEVTEDEIKQFISKQ 506
           VPVAFVVKSK SE++ED++KQF+SKQ
Sbjct: 501 VPVAFVVKSKDSELSEDDVKQFVSKQ 524

BLAST of MS016213 vs. TAIR 10
Match: AT1G65060.1 (4-coumarate:CoA ligase 3 )

HSP 1 Score: 688.7 bits (1776), Expect = 3.7e-198
Identity = 345/536 (64.37%), Postives = 429/536 (80.04%), Query Frame = 0

Query: 7   PNDFIFRSKLPDIYIPKHLPLHSYCLHENAAKIGHRTCLINGVTGDSYTYADVDLTARKV 66
           P   IFRSKLPDI IP HLPLH+YC  E  + +  + CLI G TG SYTY +  L  R+V
Sbjct: 30  PTPRIFRSKLPDIDIPNHLPLHTYCF-EKLSSVSDKPCLIVGSTGKSYTYGETHLICRRV 89

Query: 67  ASGLNKLGIAQGDVLMLLLPNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQAKGSKA 126
           ASGL KLGI +GDV+M+LL NS EFVF+F+GAS  GA+ T ANPF+T+ E+ KQ K S A
Sbjct: 90  ASGLYKLGIRKGDVIMILLQNSAEFVFSFMGASMIGAVSTTANPFYTSQELYKQLKSSGA 149

Query: 127 KLIITQASYYDKVKEITEELPDVKIMTVDSP-PDGCLSFSDLTQADERE--IPAVDIGPD 186
           KLIIT + Y DK+K + E L    ++T D P P+ CL FS L   DE       VDIG D
Sbjct: 150 KLIITHSQYVDKLKNLGENL---TLITTDEPTPENCLPFSTLITDDETNPFQETVDIGGD 209

Query: 187 DVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFRNDDVILCVLPLFHIYSL 246
           D  ALP+SSGTTGLPKGV+LTHK L+TSVAQQVDG+NPNLY +++DVILCVLPLFHIYSL
Sbjct: 210 DAAALPFSSGTTGLPKGVVLTHKSLITSVAQQVDGDNPNLYLKSNDVILCVLPLFHIYSL 269

Query: 247 NSVLLCGLRAGATILILPKFEIASLLQLVQKYRVSIAPVVPPIVLAIAKSPDLDKYDLSS 306
           NSVLL  LR+GAT+L++ KFEI +LL L+Q++RV+IA +VPP+V+A+AK+P ++ YDLSS
Sbjct: 270 NSVLLNSLRSGATVLLMHKFEIGALLDLIQRHRVTIAALVPPLVIALAKNPTVNSYDLSS 329

Query: 307 IRVLKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPGACG 366
           +R + SG APLGKEL+D++R + P+A+LGQGYGMTEAGPVL+M L FAKEP P K G+CG
Sbjct: 330 VRFVLSGAAPLGKELQDSLRRRLPQAILGQGYGMTEAGPVLSMSLGFAKEPIPTKSGSCG 389

Query: 367 TVVRNAEMKIVDTENGASLPRNTPGEICIRGDQIMKGYLNNPEATVATIDADGWLHTGDI 426
           TVVRNAE+K+V  E   SL  N PGEICIRG QIMK YLN+PEAT ATID +GWLHTGDI
Sbjct: 390 TVVRNAELKVVHLETRLSLGYNQPGEICIRGQQIMKEYLNDPEATSATIDEEGWLHTGDI 449

Query: 427 GFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVITDAAVVPMKDEQAGEVPVA 486
           G++D+DDE+FIVDRLKE+IK+KGFQV PAELE+LL+ H  I DAAVVP  DE AGEVPVA
Sbjct: 450 GYVDEDDEIFIVDRLKEVIKFKGFQVPPAELESLLINHHSIADAAVVPQNDEVAGEVPVA 509

Query: 487 FVVKSKSSEVTEDEIKQFISKQQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKL 540
           FVV+S  +++TE+++K++++K QVVFYKR+++VFF+ +IPKSPSGKILRK+L+AKL
Sbjct: 510 FVVRSNGNDITEEDVKEYVAK-QVVFYKRLHKVFFVASIPKSPSGKILRKDLKAKL 560

BLAST of MS016213 vs. TAIR 10
Match: AT3G21230.1 (4-coumarate:CoA ligase 5 )

HSP 1 Score: 674.5 bits (1739), Expect = 7.3e-194
Identity = 337/544 (61.95%), Postives = 421/544 (77.39%), Query Frame = 0

Query: 8   NDFIFRSKLPDIYIPKHLPLHSYCLHE---NAAKIGHRTCLINGVTGDSYTYADVDLTAR 67
           +DFIFRSKLPDI+IP HLPL  Y       +       TC+I+G TG   TYADV    R
Sbjct: 25  HDFIFRSKLPDIFIPNHLPLTDYVFQRFSGDGDGDSSTTCIIDGATGRILTYADVQTNMR 84

Query: 68  KVASGLNKLGIAQGDVLMLLLPNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQAKGS 127
           ++A+G+++LGI  GDV+MLLLPNSPEF  +FL  +Y GA+ T ANPF+T  EIAKQAK S
Sbjct: 85  RIAAGIHRLGIRHGDVVMLLLPNSPEFALSFLAVAYLGAVSTTANPFYTQPEIAKQAKAS 144

Query: 128 KAKLIITQASYYDKVKEITEELPDVKIMTVD---------SPPDGCLSFSDLTQADEREI 187
            AK+IIT+    DK+  +  +   V I+ +D         S  DGC+SF++LTQADE E+
Sbjct: 145 AAKMIITKKCLVDKLTNLKND--GVLIVCLDDDGDNGVVSSSDDGCVSFTELTQADETEL 204

Query: 188 PAVDIGPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFRNDDVILCVL 247
               I P+D VA+PYSSGTTGLPKGVM+THKGLVTS+AQ+VDGENPNL F  +DVILC L
Sbjct: 205 LKPKISPEDTVAMPYSSGTTGLPKGVMITHKGLVTSIAQKVDGENPNLNFTANDVILCFL 264

Query: 248 PLFHIYSLNSVLLCGLRAGATILILPKFEIASLLQLVQKYRVSIAPVVPPIVLAIAKSPD 307
           P+FHIY+L++++L  +R GA +LI+P+FE+  +++L+Q+Y+V++ PV PP+VLA  KSP+
Sbjct: 265 PMFHIYALDALMLSAMRTGAALLIVPRFELNLVMELIQRYKVTVVPVAPPVVLAFIKSPE 324

Query: 308 LDKYDLSSIRVLKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPF 367
            ++YDLSS+R++ SG A L KELED VR KFP A+ GQGYGMTE+G V    LAFAK PF
Sbjct: 325 TERYDLSSVRIMLSGAATLKKELEDAVRLKFPNAIFGQGYGMTESGTV-AKSLAFAKNPF 384

Query: 368 PMKPGACGTVVRNAEMKIVDTENGASLPRNTPGEICIRGDQIMKGYLNNPEATVATIDAD 427
             K GACGTV+RNAEMK+VDTE G SLPRN  GEIC+RG Q+MKGYLN+PEAT  TID D
Sbjct: 385 KTKSGACGTVIRNAEMKVVDTETGISLPRNKSGEICVRGHQLMKGYLNDPEATARTIDKD 444

Query: 428 GWLHTGDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVITDAAVVPMKDE 487
           GWLHTGDIGF+DDDDE+FIVDRLKELIK+KG+QVAPAELEALL++HP I DAAVV MKDE
Sbjct: 445 GWLHTGDIGFVDDDDEIFIVDRLKELIKFKGYQVAPAELEALLISHPSIDDAAVVAMKDE 504

Query: 488 QAGEVPVAFVVKSKSSEVTEDEIKQFISKQQVVFYKRINRVFFIDAIPKSPSGKILRKEL 540
            A EVPVAFV +S+ S++TED++K +++K QVV YKRI  VFFI+ IPK+ SGKILRK+L
Sbjct: 505 VADEVPVAFVARSQGSQLTEDDVKSYVNK-QVVHYKRIKMVFFIEVIPKAVSGKILRKDL 564