MS016193 (gene) Bitter gourd (TR) v1

Overview
NameMS016193
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionGrowth-regulating factor
Locationscaffold9_2: 999226 .. 1000550 (+)
RNA-Seq ExpressionMS016193
SyntenyMS016193
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAATTCAAGCCCCAAGGCCTGCACCTCTCCTTTCACAGCAACTCAATGGCAAGAACTGGAACACCAAGCTCTGATCTTCAAATACATGGTTTCAGGAGTACCAATCCCTCCTGATCTAATCTTTTCTGTGAAAAGAAGCTTGGATTCCTCCATTTCTGCAAGACTCTTCCCTCACCAACCCAGTAAGTCGCCATTGCCAAGGCCTTACAAAACAGCTGCTCCCTCCGTGCCATTTTTTTACCTCAGTTTTCAGATGCAAGCATGTTATAGTGTCTGACTGTTTGATTGATTGTGTTTTTTTCTGCAGTTGGATGGGGATGTTTTGAGATGGGTTTTGGCAGAAAAGCAGACCCAGAGCCAGGAAGGTGCAGAAGAACCGATGGGAAAAAATGGAGATGCTCAAAAGAAGCTTATCCAGACTCCAAATACTGCGAGAGGCACATGCATAGAGGCAGAAACCGTTCAAGAAAGCCTGTGGAAATCTCTTCAACGCCCTCAACTCAAACTCAGCCACCGCCGCCTTTAATCCTCACTTCAAACAGAAGCCTCTCCTCCTCTTCCACTCCCACCACATCTTCTTACTCTCTCTCTACACTCTCTTCTTCTTCTTCCTCACTGACCTGTGAAACTCAATCTTATCACCAAGCTGCTGCTGCTTATCATGAATCCTCCATTCATCCCTTTCTCTATCCTCAATCTTCCTCCTCCAGACCTCCATGTTCTGATTTCTCTCCTATTAACACAAACCCTCAACTCTTTTTGTACTCGGGGACTCATGCTCAGGCTGGCAAAGATTACAGGTTGGTGCGAAATAATGGTTAAAAAGATGTTACCTTTTCTTTTAATGGGTTGTTTTCCTTGAAGTTTGATGATTGATATTATAAATAATGTTGTTGATATAAAGTATTAATTAATATGACATATTAAGATTATTGGGTCGTGTAAAGTAGGGACATGGATGGTCCAAGAGAGGGTGTGGGCGTGCAGTCGTTTTTTCCCTCAATTGCAGATTCTGCAAGAAGCTTAGCTGAGGTTTATAATCAGCCAATGGCAATGGATTCTTATAAACAATCTGTTTCCCAAAGCCACCAAATTTACAGTTCGAAACAAGCAAGGCACCAACAAGAGCAACACTGCTTTGTTTTGGGCACAGACTTCAACAAACCATCGAGATCGGCTAAAACAGAGGAGGAAGAAGAAGAAGATGCAGAGACCCAGAAGCCACTACTCCACTTCTTTGAGGATTGCCCCCCAAAAAATTCAGATTCCTGGCTTGATTTAGCCTCAAGTTCAAGTATACAAAGTGGAAATTTTATTGGGTTA

mRNA sequence

ATGAATTCAAGCCCCAAGGCCTGCACCTCTCCTTTCACAGCAACTCAATGGCAAGAACTGGAACACCAAGCTCTGATCTTCAAATACATGGTTTCAGGAGTACCAATCCCTCCTGATCTAATCTTTTCTGTGAAAAGAAGCTTGGATTCCTCCATTTCTGCAAGACTCTTCCCTCACCAACCCATTGGATGGGGATGTTTTGAGATGGGTTTTGGCAGAAAAGCAGACCCAGAGCCAGGAAGGTGCAGAAGAACCGATGGGAAAAAATGGAGATGCTCAAAAGAAGCTTATCCAGACTCCAAATACTGCGAGAGGCACATGCATAGAGGCAGAAACCGTTCAAGAAAGCCTGTGGAAATCTCTTCAACGCCCTCAACTCAAACTCAGCCACCGCCGCCTTTAATCCTCACTTCAAACAGAAGCCTCTCCTCCTCTTCCACTCCCACCACATCTTCTTACTCTCTCTCTACACTCTCTTCTTCTTCTTCCTCACTGACCTGTGAAACTCAATCTTATCACCAAGCTGCTGCTGCTTATCATGAATCCTCCATTCATCCCTTTCTCTATCCTCAATCTTCCTCCTCCAGACCTCCATGTTCTGATTTCTCTCCTATTAACACAAACCCTCAACTCTTTTTGTACTCGGGGACTCATGCTCAGGCTGGCAAAGATTACAGTAGGGACATGGATGGTCCAAGAGAGGGTGTGGGCGTGCAGTCGTTTTTTCCCTCAATTGCAGATTCTGCAAGAAGCTTAGCTGAGGTTTATAATCAGCCAATGGCAATGGATTCTTATAAACAATCTGTTTCCCAAAGCCACCAAATTTACAGTTCGAAACAAGCAAGGCACCAACAAGAGCAACACTGCTTTGTTTTGGGCACAGACTTCAACAAACCATCGAGATCGGCTAAAACAGAGGAGGAAGAAGAAGAAGATGCAGAGACCCAGAAGCCACTACTCCACTTCTTTGAGGATTGCCCCCCAAAAAATTCAGATTCCTGGCTTGATTTAGCCTCAAGTTCAAGTATACAAAGTGGAAATTTTATTGGGTTA

Coding sequence (CDS)

ATGAATTCAAGCCCCAAGGCCTGCACCTCTCCTTTCACAGCAACTCAATGGCAAGAACTGGAACACCAAGCTCTGATCTTCAAATACATGGTTTCAGGAGTACCAATCCCTCCTGATCTAATCTTTTCTGTGAAAAGAAGCTTGGATTCCTCCATTTCTGCAAGACTCTTCCCTCACCAACCCATTGGATGGGGATGTTTTGAGATGGGTTTTGGCAGAAAAGCAGACCCAGAGCCAGGAAGGTGCAGAAGAACCGATGGGAAAAAATGGAGATGCTCAAAAGAAGCTTATCCAGACTCCAAATACTGCGAGAGGCACATGCATAGAGGCAGAAACCGTTCAAGAAAGCCTGTGGAAATCTCTTCAACGCCCTCAACTCAAACTCAGCCACCGCCGCCTTTAATCCTCACTTCAAACAGAAGCCTCTCCTCCTCTTCCACTCCCACCACATCTTCTTACTCTCTCTCTACACTCTCTTCTTCTTCTTCCTCACTGACCTGTGAAACTCAATCTTATCACCAAGCTGCTGCTGCTTATCATGAATCCTCCATTCATCCCTTTCTCTATCCTCAATCTTCCTCCTCCAGACCTCCATGTTCTGATTTCTCTCCTATTAACACAAACCCTCAACTCTTTTTGTACTCGGGGACTCATGCTCAGGCTGGCAAAGATTACAGTAGGGACATGGATGGTCCAAGAGAGGGTGTGGGCGTGCAGTCGTTTTTTCCCTCAATTGCAGATTCTGCAAGAAGCTTAGCTGAGGTTTATAATCAGCCAATGGCAATGGATTCTTATAAACAATCTGTTTCCCAAAGCCACCAAATTTACAGTTCGAAACAAGCAAGGCACCAACAAGAGCAACACTGCTTTGTTTTGGGCACAGACTTCAACAAACCATCGAGATCGGCTAAAACAGAGGAGGAAGAAGAAGAAGATGCAGAGACCCAGAAGCCACTACTCCACTTCTTTGAGGATTGCCCCCCAAAAAATTCAGATTCCTGGCTTGATTTAGCCTCAAGTTCAAGTATACAAAGTGGAAATTTTATTGGGTTA

Protein sequence

MNSSPKACTSPFTATQWQELEHQALIFKYMVSGVPIPPDLIFSVKRSLDSSISARLFPHQPIGWGCFEMGFGRKADPEPGRCRRTDGKKWRCSKEAYPDSKYCERHMHRGRNRSRKPVEISSTPSTQTQPPPPLILTSNRSLSSSSTPTTSSYSLSTLSSSSSSLTCETQSYHQAAAAYHESSIHPFLYPQSSSSRPPCSDFSPINTNPQLFLYSGTHAQAGKDYSRDMDGPREGVGVQSFFPSIADSARSLAEVYNQPMAMDSYKQSVSQSHQIYSSKQARHQQEQHCFVLGTDFNKPSRSAKTEEEEEEDAETQKPLLHFFEDCPPKNSDSWLDLASSSSIQSGNFIGL
Homology
BLAST of MS016193 vs. NCBI nr
Match: XP_022152689.1 (growth-regulating factor 5-like isoform X1 [Momordica charantia])

HSP 1 Score: 689.9 bits (1779), Expect = 1.2e-194
Identity = 350/351 (99.72%), Postives = 350/351 (99.72%), Query Frame = 0

Query: 1   MNSSPKACTSPFTATQWQELEHQALIFKYMVSGVPIPPDLIFSVKRSLDSSISARLFPHQ 60
           MNSSPKACTSPFTATQWQELEHQALIFKYMVSGVPIPPDLIFSVKRSLDSSISARLFPHQ
Sbjct: 1   MNSSPKACTSPFTATQWQELEHQALIFKYMVSGVPIPPDLIFSVKRSLDSSISARLFPHQ 60

Query: 61  PIGWGCFEMGFGRKADPEPGRCRRTDGKKWRCSKEAYPDSKYCERHMHRGRNRSRKPVEI 120
           PIGWGCFEMGFGRKADPEPGRCRRTDGKKWRCSKEAYPDSKYCERHMHRGRNRSRKPVEI
Sbjct: 61  PIGWGCFEMGFGRKADPEPGRCRRTDGKKWRCSKEAYPDSKYCERHMHRGRNRSRKPVEI 120

Query: 121 SSTPSTQTQPPPPLILTSNRSLSSSSTPTTSSYSLSTLSSSSSSLTCETQSYHQAAAAYH 180
           SSTPSTQTQPPPPLILTSNRSLSSSSTPTTSSYSLSTLSSSSSSLTCETQSYHQAAAAYH
Sbjct: 121 SSTPSTQTQPPPPLILTSNRSLSSSSTPTTSSYSLSTLSSSSSSLTCETQSYHQAAAAYH 180

Query: 181 ESSIHPFLYPQSSSSRPPCSDFSPINTNPQLFLYSGTHAQAGKDYSRDMDGPREGVGVQS 240
           ESSIHPFLYPQSSSSRPPCSDFSPINTNPQLFLYSGT AQAGKDYSRDMDGPREGVGVQS
Sbjct: 181 ESSIHPFLYPQSSSSRPPCSDFSPINTNPQLFLYSGT-AQAGKDYSRDMDGPREGVGVQS 240

Query: 241 FFPSIADSARSLAEVYNQPMAMDSYKQSVSQSHQIYSSKQARHQQEQHCFVLGTDFNKPS 300
           FFPSIADSARSLAEVYNQPMAMDSYKQSVSQSHQIYSSKQARHQQEQHCFVLGTDFNKPS
Sbjct: 241 FFPSIADSARSLAEVYNQPMAMDSYKQSVSQSHQIYSSKQARHQQEQHCFVLGTDFNKPS 300

Query: 301 RSAKTEEEEEEDAETQKPLLHFFEDCPPKNSDSWLDLASSSSIQSGNFIGL 352
           RSAKTEEEEEEDAETQKPLLHFFEDCPPKNSDSWLDLASSSSIQSGNFIGL
Sbjct: 301 RSAKTEEEEEEDAETQKPLLHFFEDCPPKNSDSWLDLASSSSIQSGNFIGL 350

BLAST of MS016193 vs. NCBI nr
Match: XP_022152690.1 (growth-regulating factor 5-like isoform X2 [Momordica charantia])

HSP 1 Score: 683.7 bits (1763), Expect = 8.3e-193
Identity = 349/351 (99.43%), Postives = 349/351 (99.43%), Query Frame = 0

Query: 1   MNSSPKACTSPFTATQWQELEHQALIFKYMVSGVPIPPDLIFSVKRSLDSSISARLFPHQ 60
           MNSSPKACTSPFTATQWQELEHQALIFKYMVSGVPIPPDLIFSVKRSLDSSISARLFPHQ
Sbjct: 1   MNSSPKACTSPFTATQWQELEHQALIFKYMVSGVPIPPDLIFSVKRSLDSSISARLFPHQ 60

Query: 61  PIGWGCFEMGFGRKADPEPGRCRRTDGKKWRCSKEAYPDSKYCERHMHRGRNRSRKPVEI 120
           PIGWGCFEMGFGRKADPEPGRCRRTDGKKWRCSKEAYPDSKYCERHMHRGRNRSRKPVEI
Sbjct: 61  PIGWGCFEMGFGRKADPEPGRCRRTDGKKWRCSKEAYPDSKYCERHMHRGRNRSRKPVEI 120

Query: 121 SSTPSTQTQPPPPLILTSNRSLSSSSTPTTSSYSLSTLSSSSSSLTCETQSYHQAAAAYH 180
           SSTPSTQTQPPPPLILTSNRSLSSSSTPTTSSYSLSTLSSSSSSLTCETQSYHQAAAAYH
Sbjct: 121 SSTPSTQTQPPPPLILTSNRSLSSSSTPTTSSYSLSTLSSSSSSLTCETQSYHQAAAAYH 180

Query: 181 ESSIHPFLYPQSSSSRPPCSDFSPINTNPQLFLYSGTHAQAGKDYSRDMDGPREGVGVQS 240
           ESSIHPFLYPQSSSSRPPCSDFSPINTNPQLFLYSGT AQAGKDY RDMDGPREGVGVQS
Sbjct: 181 ESSIHPFLYPQSSSSRPPCSDFSPINTNPQLFLYSGT-AQAGKDY-RDMDGPREGVGVQS 240

Query: 241 FFPSIADSARSLAEVYNQPMAMDSYKQSVSQSHQIYSSKQARHQQEQHCFVLGTDFNKPS 300
           FFPSIADSARSLAEVYNQPMAMDSYKQSVSQSHQIYSSKQARHQQEQHCFVLGTDFNKPS
Sbjct: 241 FFPSIADSARSLAEVYNQPMAMDSYKQSVSQSHQIYSSKQARHQQEQHCFVLGTDFNKPS 300

Query: 301 RSAKTEEEEEEDAETQKPLLHFFEDCPPKNSDSWLDLASSSSIQSGNFIGL 352
           RSAKTEEEEEEDAETQKPLLHFFEDCPPKNSDSWLDLASSSSIQSGNFIGL
Sbjct: 301 RSAKTEEEEEEDAETQKPLLHFFEDCPPKNSDSWLDLASSSSIQSGNFIGL 349

BLAST of MS016193 vs. NCBI nr
Match: XP_022152691.1 (growth-regulating factor 5-like isoform X3 [Momordica charantia])

HSP 1 Score: 671.4 bits (1731), Expect = 4.3e-189
Identity = 340/342 (99.42%), Postives = 341/342 (99.71%), Query Frame = 0

Query: 1   MNSSPKACTSPFTATQWQELEHQALIFKYMVSGVPIPPDLIFSVKRSLDSSISARLFPHQ 60
           MNSSPKACTSPFTATQWQELEHQALIFKYMVSGVPIPPDLIFSVKRSLDSSISARLFPHQ
Sbjct: 1   MNSSPKACTSPFTATQWQELEHQALIFKYMVSGVPIPPDLIFSVKRSLDSSISARLFPHQ 60

Query: 61  PIGWGCFEMGFGRKADPEPGRCRRTDGKKWRCSKEAYPDSKYCERHMHRGRNRSRKPVEI 120
           PIGWGCFEMGFGRKADPEPGRCRRTDGKKWRCSKEAYPDSKYCERHMHRGRNRSRKPVEI
Sbjct: 61  PIGWGCFEMGFGRKADPEPGRCRRTDGKKWRCSKEAYPDSKYCERHMHRGRNRSRKPVEI 120

Query: 121 SSTPSTQTQPPPPLILTSNRSLSSSSTPTTSSYSLSTLSSSSSSLTCETQSYHQAAAAYH 180
           SSTPSTQTQPPPPLILTSNRSLSSSSTPTTSSYSLSTLSSSSSSLTCETQSYHQAAAAYH
Sbjct: 121 SSTPSTQTQPPPPLILTSNRSLSSSSTPTTSSYSLSTLSSSSSSLTCETQSYHQAAAAYH 180

Query: 181 ESSIHPFLYPQSSSSRPPCSDFSPINTNPQLFLYSGTHAQAGKDYSRDMDGPREGVGVQS 240
           ESSIHPFLYPQSSSSRPPCSDFSPINTNPQLFLYSGT AQAGKDYSRDMDGPREGVGVQS
Sbjct: 181 ESSIHPFLYPQSSSSRPPCSDFSPINTNPQLFLYSGT-AQAGKDYSRDMDGPREGVGVQS 240

Query: 241 FFPSIADSARSLAEVYNQPMAMDSYKQSVSQSHQIYSSKQARHQQEQHCFVLGTDFNKPS 300
           FFPSIADSARSLAEVYNQPMAMDSYKQSVSQSHQIYSSKQARHQQEQHCFVLGTDFNKPS
Sbjct: 241 FFPSIADSARSLAEVYNQPMAMDSYKQSVSQSHQIYSSKQARHQQEQHCFVLGTDFNKPS 300

Query: 301 RSAKTEEEEEEDAETQKPLLHFFEDCPPKNSDSWLDLASSSS 343
           RSAKTEEEEEEDAETQKPLLHFFEDCPPKNSDSWLDLASSS+
Sbjct: 301 RSAKTEEEEEEDAETQKPLLHFFEDCPPKNSDSWLDLASSSN 341

BLAST of MS016193 vs. NCBI nr
Match: XP_038879715.1 (growth-regulating factor 5 isoform X1 [Benincasa hispida])

HSP 1 Score: 593.2 bits (1528), Expect = 1.5e-165
Identity = 313/355 (88.17%), Postives = 326/355 (91.83%), Query Frame = 0

Query: 1   MNSSPKACTSPFTATQWQELEHQALIFKYMVSGVPIPPDLIFSVKRSLDSSISARLFPHQ 60
           MNSSPK CTSPFTATQWQELEHQALIFKYMVSGVPIPPDLIFSVKRSLDSSISARLFPHQ
Sbjct: 1   MNSSPKTCTSPFTATQWQELEHQALIFKYMVSGVPIPPDLIFSVKRSLDSSISARLFPHQ 60

Query: 61  PIGWGCFEMGFGRKADPEPGRCRRTDGKKWRCSKEAYPDSKYCERHMHRGRNRSRKPVEI 120
           PIGWGCFEMGFGRK DPEPGRCRRTDGKKWRCSKEAYPDSKYCERHMHRGRNRSRKPVEI
Sbjct: 61  PIGWGCFEMGFGRKTDPEPGRCRRTDGKKWRCSKEAYPDSKYCERHMHRGRNRSRKPVEI 120

Query: 121 SSTPSTQTQPPPPLILTSNRSLSSSSTPTTSSYSLSTL-SSSSSSLTCETQSYHQAAAAY 180
           SSTP TQT  P PLILTS R+L  SSTPT+SSY LSTL SSSSSSLTCETQSY+Q   AY
Sbjct: 121 SSTPPTQT--PSPLILTSTRTL--SSTPTSSSYPLSTLSSSSSSSLTCETQSYNQPPPAY 180

Query: 181 HESSIHPFLYPQSSSSRPPCSDFSPINTNPQLFLYSGTHAQAGKDYSRDMDGPREGVGVQ 240
           HESSIHPFLY Q SSSRPPCSDFS  NTNPQLFLYSGTH+QA KDY RD+ GPREGVG+Q
Sbjct: 181 HESSIHPFLYHQ-SSSRPPCSDFSSNNTNPQLFLYSGTHSQADKDY-RDISGPREGVGMQ 240

Query: 241 SFFPSIADSARSLAEVYNQPMAMDSYK---QSVSQSHQIYSSKQARHQQEQHCFVLGTDF 300
           +FFPS+ADSARS+AEVY+QP+ MDSYK   Q VSQSHQIYSSKQAR QQEQHCFVLGTDF
Sbjct: 241 AFFPSVADSARSMAEVYSQPIGMDSYKGCSQFVSQSHQIYSSKQARQQQEQHCFVLGTDF 300

Query: 301 NKPSRSAKTEEEEEEDAETQKPLLHFFEDCPPKNSDSWLDLASSSSIQSGNFIGL 352
           NK SRSAKT EEE+EDAETQKPLLHFFEDCPPKNSDSWLDLAS+SSIQSGNFIGL
Sbjct: 301 NKSSRSAKT-EEEKEDAETQKPLLHFFEDCPPKNSDSWLDLASNSSIQSGNFIGL 348

BLAST of MS016193 vs. NCBI nr
Match: XP_023525289.1 (growth-regulating factor 5-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 592.0 bits (1525), Expect = 3.3e-165
Identity = 310/356 (87.08%), Postives = 326/356 (91.57%), Query Frame = 0

Query: 1   MNSSPKACTSPFTATQWQELEHQALIFKYMVSGVPIPPDLIFSVKRSLDSSISARLFPHQ 60
           MNS PK C SPFT TQWQELEHQALIFKYMVSGVPIPPDLIFSVKRSLDSSISARLFPHQ
Sbjct: 1   MNSGPKPCISPFTPTQWQELEHQALIFKYMVSGVPIPPDLIFSVKRSLDSSISARLFPHQ 60

Query: 61  PIGWGCFEMGFGRKADPEPGRCRRTDGKKWRCSKEAYPDSKYCERHMHRGRNRSRKPVEI 120
           PIGWGCFEMGFGRKADPEPGRCRRTDGKKWRCSKEAYPDSKYCERHMHRGRNRSRKPVEI
Sbjct: 61  PIGWGCFEMGFGRKADPEPGRCRRTDGKKWRCSKEAYPDSKYCERHMHRGRNRSRKPVEI 120

Query: 121 SSTPSTQTQPPPPLILTSNRSLSSSSTPTTSSYSLSTLSSSSSSLTCETQSYHQ--AAAA 180
           SSTP  QT  PPPLILTS RSLS+SS PT+SSYSLSTL SSSSSLTCETQSYHQ  A AA
Sbjct: 121 SSTPPAQT--PPPLILTSTRSLSASS-PTSSSYSLSTL-SSSSSLTCETQSYHQTPAPAA 180

Query: 181 YHESSIHPFLYPQSSSSRPPCSDFSPINTNPQLFLYSGTHAQAGKDYSRDMDGPREGVGV 240
           Y ESSIHP+LYPQSSSSRPPCSDFSP +TNPQLFLYSGTH QA KDY RD++GPREGVGV
Sbjct: 181 YQESSIHPYLYPQSSSSRPPCSDFSPNSTNPQLFLYSGTHPQADKDY-RDINGPREGVGV 240

Query: 241 QSFFPSIADSARSLAEVYNQPMAMDSYK---QSVSQSHQIYSSKQARHQQEQHCFVLGTD 300
           Q+FFPS+ADSARS+ EVY+QP+ MDSYK    +VS SHQIYSSKQAR QQEQHCFVLGTD
Sbjct: 241 QAFFPSVADSARSIGEVYSQPIGMDSYKGFSHAVSNSHQIYSSKQARQQQEQHCFVLGTD 300

Query: 301 FNKPSRSAKTEEEEEEDAETQKPLLHFFEDCPPKNSDSWLDLASSSSIQSGNFIGL 352
           FNK +RSAKT  EEE+DAETQKPLLHFFEDCPPKNSDSWLDLA++SSIQSGNFIGL
Sbjct: 301 FNKSTRSAKT--EEEDDAETQKPLLHFFEDCPPKNSDSWLDLATNSSIQSGNFIGL 349

BLAST of MS016193 vs. ExPASy Swiss-Prot
Match: Q8L8A6 (Growth-regulating factor 5 OS=Arabidopsis thaliana OX=3702 GN=GRF5 PE=1 SV=1)

HSP 1 Score: 249.2 bits (635), Expect = 6.8e-65
Identity = 167/385 (43.38%), Postives = 225/385 (58.44%), Query Frame = 0

Query: 11  PFTATQWQELEHQALIFKYMVSGVPIPPDLIFSVKRSLDSSISARLFPHQPIGWGCFEMG 70
           PFT TQW+ELEHQALI+KYMVSGVP+PP+LIFS++RSLD+S+ +RL PHQ +GWGC++MG
Sbjct: 16  PFTPTQWEELEHQALIYKYMVSGVPVPPELIFSIRRSLDTSLVSRLLPHQSLGWGCYQMG 75

Query: 71  FGRKADPEPGRCRRTDGKKWRCSKEAYPDSKYCERHMHRGRNRSRKPVEISSTPSTQTQP 130
           FGRK DPEPGRCRRTDGKKWRCS+EAYPDSKYCE+HMHRGRNR+RK ++ + T +T    
Sbjct: 76  FGRKPDPEPGRCRRTDGKKWRCSREAYPDSKYCEKHMHRGRNRARKSLDQNQTTTTPLTS 135

Query: 131 PPPLILTSNRSLSSSSTPTTSSYSLSTLSSSSSSLTCETQSYHQAAAAYHES-----SIH 190
           P      +N    + S+ ++S+ S +T S+SSSS+   + S          +     + H
Sbjct: 136 PSLSFTNNNNPSPTLSSSSSSNSSSTTYSASSSSMDAYSNSNRFGLGGSSSNTRGYFNSH 195

Query: 191 PFLYPQSSSSRPPCSDFSPINTNPQLFLYSGTH----------AQAGKDYSRDMDGPRE- 250
              YP  S+S  P      ++    L L+  T+          A   KD+ R   G  E 
Sbjct: 196 SLDYPYPSTS--PKQQQQTLHHASALSLHQNTNSTSQFNVLASATDHKDF-RYFQGIGER 255

Query: 251 --GVGVQSFFP----SIADS-----ARSLAEVYNQPMAMDSYKQSVSQSHQIYSSKQA-- 310
             GVG ++FFP    S  DS      + LA V N P    S   +    H  YSS  +  
Sbjct: 256 VGGVGERTFFPEASRSFQDSPYHHHQQPLATVMNDPYHHCSTDHNKIDHHHTYSSSSSSQ 315

Query: 311 -----RHQQEQHCFVLGTD-FNKPSRSA--------KTEEEEEEDA--ETQKPLLHFFED 348
                   ++Q CFVLG D FNKP+RS         + +EE+E+D+   ++K L HFF +
Sbjct: 316 HLHHDHDHRQQQCFVLGADMFNKPTRSVLANSSRQDQNQEEDEKDSSESSKKSLHHFFGE 375

BLAST of MS016193 vs. ExPASy Swiss-Prot
Match: A2XA73 (Growth-regulating factor 1 OS=Oryza sativa subsp. indica OX=39946 GN=GRF1 PE=2 SV=1)

HSP 1 Score: 209.9 bits (533), Expect = 4.6e-53
Identity = 157/375 (41.87%), Postives = 203/375 (54.13%), Query Frame = 0

Query: 11  PFTATQWQELEHQALIFKYMVSGVPIPPDLIFSVKRS--LDSSIS---ARLFPHQP-IGW 70
           PFTA+QWQELEHQALI+KYM SG PIP DLI  ++RS  LDS+++   +  FP QP +GW
Sbjct: 18  PFTASQWQELEHQALIYKYMASGTPIPSDLILPLRRSFLLDSALATSPSLAFPPQPSLGW 77

Query: 71  GCFEMGFGRKA-DPEPGRCRRTDGKKWRCSKEAYPDSKYCERHMHRGRNRSRKPVEISST 130
           GCF MGFGRKA DPEPGRCRRTDGKKWRCSKEAYPDSKYCE+HMHRG+NRSRKPVE+   
Sbjct: 78  GCFGMGFGRKAEDPEPGRCRRTDGKKWRCSKEAYPDSKYCEKHMHRGKNRSRKPVEM--- 137

Query: 131 PSTQTQPPPPLILTSNRSLSSSSTPTTSSYSLSTLSSSSSSLTCETQSYH---------- 190
            S  T PPP    TS  S SS+    T++ + S   S S     +   Y           
Sbjct: 138 -SLATPPPPSSSATSAASNSSAGVAPTTTTTSSPAPSYSRPAPHDAAPYQALYGGPYAAA 197

Query: 191 ----QAAAAYHESSIHPF-LYPQSSSSRPPCSDFSPINTNPQLFLYS-------GTHAQA 250
                AAAAYH + + PF L+  ++   PP S +S    + + + Y        G HA  
Sbjct: 198 TARTPAAAAYH-AQVSPFHLHIDTTHPHPPPSYYS---MDHKEYAYGHATKEVHGEHAFF 257

Query: 251 GKDYSRDMDGPREGVGVQSFFPSIADSARSLAEVY---------NQPMAMDSYKQSVSQS 310
                R+      G G   F     +  +S   ++           P A+D  K+   + 
Sbjct: 258 SDGTEREHHHAAAGHGQWQFKQLGMEPKQSTTPLFPGAGYGHTAASPYAIDLSKEDDDEK 317

Query: 311 HQIYSSKQARHQQEQHCFVLGTD--FNKPSRSAKTEEEEEEDAETQKPLLHFFEDCP-PK 344
            +    +Q + QQ+QHCF+LG D    KP+         +  A  QKPL HFF++ P  K
Sbjct: 318 ER----RQQQQQQQQHCFLLGADLRLEKPA-------GHDHAAAAQKPLRHFFDEWPHEK 373

BLAST of MS016193 vs. ExPASy Swiss-Prot
Match: Q6AWY8 (Growth-regulating factor 1 OS=Oryza sativa subsp. japonica OX=39947 GN=GRF1 PE=3 SV=1)

HSP 1 Score: 207.2 bits (526), Expect = 3.0e-52
Identity = 156/375 (41.60%), Postives = 202/375 (53.87%), Query Frame = 0

Query: 11  PFTATQWQELEHQALIFKYMVSGVPIPPDLIFSVKRS--LDSSIS---ARLFPHQP-IGW 70
           PFTA+QWQELEHQALI+KYM SG PIP DLI  ++RS  LDS+++   +  FP QP +GW
Sbjct: 18  PFTASQWQELEHQALIYKYMASGTPIPSDLILPLRRSFLLDSALATSPSLAFPPQPSLGW 77

Query: 71  GCFEMGFGRKA-DPEPGRCRRTDGKKWRCSKEAYPDSKYCERHMHRGRNRSRKPVEISST 130
           GCF MGFGRKA DPEPGRCRRTDGKKWRCSKEAYPDSKYCE+HMHRG+NRSRKPVE+   
Sbjct: 78  GCFGMGFGRKAEDPEPGRCRRTDGKKWRCSKEAYPDSKYCEKHMHRGKNRSRKPVEM--- 137

Query: 131 PSTQTQPPPPLILTSNRSLSSSSTPTTSSYSLSTLSSSSSSLTCETQSYH---------- 190
            S  T PPP    TS  S +S+    T++ + S   S S     +   Y           
Sbjct: 138 -SLATPPPPSSSATSAASNTSAGVAPTTTTTSSPAPSYSRPAPHDAAPYQALYGGPYAAA 197

Query: 191 ----QAAAAYHESSIHPF-LYPQSSSSRPPCSDFSPINTNPQLFLYS-------GTHAQA 250
                AAAAYH + + PF L   ++   PP S +S    + + + Y        G HA  
Sbjct: 198 TARTPAAAAYH-AQVSPFHLQLDTTHPHPPPSYYS---MDHKEYAYGHATKEVHGEHAFF 257

Query: 251 GKDYSRDMDGPREGVGVQSFFPSIADSARSLAEVY---------NQPMAMDSYKQSVSQS 310
                R+      G G   F     +  +S   ++           P A+D  K+   + 
Sbjct: 258 SDGTEREHHHAAAGHGQWQFKQLGMEPKQSTTPLFPGAGYGHTAASPYAIDLSKEDDDEK 317

Query: 311 HQIYSSKQARHQQEQHCFVLGTD--FNKPSRSAKTEEEEEEDAETQKPLLHFFEDCP-PK 344
            +    +Q + QQ+QHCF+LG D    KP+         +  A  QKPL HFF++ P  K
Sbjct: 318 ER---RQQQQQQQQQHCFLLGADLRLEKPA-------GHDHAAAAQKPLRHFFDEWPHEK 374

BLAST of MS016193 vs. ExPASy Swiss-Prot
Match: A0A060D764 (Growth-regulating factor 1 OS=Zea mays OX=4577 GN=GRF1 PE=1 SV=1)

HSP 1 Score: 165.6 bits (418), Expect = 9.9e-40
Identity = 83/141 (58.87%), Postives = 106/141 (75.18%), Query Frame = 0

Query: 11  PFTATQWQELEHQALIFKYMVSGVPIPPDLIFSVKRSLDSSISARLFPHQPIGWGCFEMG 70
           PFT  Q+QELE QALI+KY+V+GVP+PPDL+  ++R LD S++ R +    +G+G +   
Sbjct: 59  PFTPAQYQELEQQALIYKYLVAGVPVPPDLVVPIRRGLD-SLATRFYGQPTLGYGPY--- 118

Query: 71  FGRKADPEPGRCRRTDGKKWRCSKEAYPDSKYCERHMHRGRNRSRKPVEISSTPSTQTQP 130
            GRK DPEPGRCRRTDGKKWRCSKEA PDSKYCERHMHRGRNRSRKPVE    P  Q+QP
Sbjct: 119 LGRKLDPEPGRCRRTDGKKWRCSKEAAPDSKYCERHMHRGRNRSRKPVETQLAP--QSQP 178

Query: 131 PPPLILTSNRSLSSSSTPTTS 152
           P    +++   L++++  TT+
Sbjct: 179 PAAAAVSAAPPLAAAAAATTN 193

BLAST of MS016193 vs. ExPASy Swiss-Prot
Match: Q6ZIK5 (Growth-regulating factor 4 OS=Oryza sativa subsp. japonica OX=39947 GN=GRF4 PE=1 SV=1)

HSP 1 Score: 162.9 bits (411), Expect = 6.4e-39
Identity = 127/342 (37.13%), Postives = 183/342 (53.51%), Query Frame = 0

Query: 11  PFTATQWQELEHQALIFKYMVSGVPIPPDLIFSVKRSLDSSISARLFPHQPIGWGCFEMG 70
           PFTA Q++ELE QALI+KY+V+GVP+PPDL+  ++R LD S++AR + H  +G+G +   
Sbjct: 64  PFTAAQYEELEQQALIYKYLVAGVPVPPDLVLPIRRGLD-SLAARFYNHPALGYGPY--- 123

Query: 71  FGRKADPEPGRCRRTDGKKWRCSKEAYPDSKYCERHMHRGRNRSRKPVEISSTPSTQTQP 130
           FG+K DPEPGRCRRTDGKKWRCSKEA PDSKYCERHMHRGRNRSRKPVE       Q+QP
Sbjct: 124 FGKKLDPEPGRCRRTDGKKWRCSKEAAPDSKYCERHMHRGRNRSRKPVETQLV--AQSQP 183

Query: 131 PPPLILTSNRSLSSSSTPTTSSYSLSTLSSSSSSLTCETQSYHQAAAAYHESS---IHPF 190
           P  ++        S++ P         L+++S+  + +  S + A A  +        P 
Sbjct: 184 PSSVV-------GSAAAP---------LAAASNGSSFQNHSLYPAIAGSNGGGGGRNMPS 243

Query: 191 LYPQSSSSRPPCSDFSPINTNPQLFLYSGTHAQAGKDYSRDMDGPREGVGVQSFFPSIA- 250
            +  +  S+    + +P         Y+      GKD      G R     QS   + A 
Sbjct: 244 SFGSALGSQLHMDNAAP---------YAAVGGGTGKDLRYTAYGTRSLADEQSQLITEAI 303

Query: 251 ----DSARSLAEVYNQPMAMDSYKQ--SVSQSHQIYSSKQARHQQEQHCFVLGTDFNKPS 310
               ++   L    N P  + SY Q  ++S   Q   S  ++ Q++   F  G D+    
Sbjct: 304 NTSIENPWRLLPSQNSPFPLSSYSQLGALSDLGQNTPSSLSKVQRQPLSF-FGNDY---- 362

Query: 311 RSAKTEEEEEEDAETQKPLLHFFEDCPPKNSDSWLDLASSSS 343
             A  +  ++E+ +T +P   FF++  PK  DSW DLA  ++
Sbjct: 364 --AAVDSVKQEN-QTLRP---FFDEW-PKGRDSWSDLADENA 362

BLAST of MS016193 vs. ExPASy TrEMBL
Match: A0A6J1DGS7 (Growth-regulating factor OS=Momordica charantia OX=3673 GN=LOC111020348 PE=3 SV=1)

HSP 1 Score: 689.9 bits (1779), Expect = 5.6e-195
Identity = 350/351 (99.72%), Postives = 350/351 (99.72%), Query Frame = 0

Query: 1   MNSSPKACTSPFTATQWQELEHQALIFKYMVSGVPIPPDLIFSVKRSLDSSISARLFPHQ 60
           MNSSPKACTSPFTATQWQELEHQALIFKYMVSGVPIPPDLIFSVKRSLDSSISARLFPHQ
Sbjct: 1   MNSSPKACTSPFTATQWQELEHQALIFKYMVSGVPIPPDLIFSVKRSLDSSISARLFPHQ 60

Query: 61  PIGWGCFEMGFGRKADPEPGRCRRTDGKKWRCSKEAYPDSKYCERHMHRGRNRSRKPVEI 120
           PIGWGCFEMGFGRKADPEPGRCRRTDGKKWRCSKEAYPDSKYCERHMHRGRNRSRKPVEI
Sbjct: 61  PIGWGCFEMGFGRKADPEPGRCRRTDGKKWRCSKEAYPDSKYCERHMHRGRNRSRKPVEI 120

Query: 121 SSTPSTQTQPPPPLILTSNRSLSSSSTPTTSSYSLSTLSSSSSSLTCETQSYHQAAAAYH 180
           SSTPSTQTQPPPPLILTSNRSLSSSSTPTTSSYSLSTLSSSSSSLTCETQSYHQAAAAYH
Sbjct: 121 SSTPSTQTQPPPPLILTSNRSLSSSSTPTTSSYSLSTLSSSSSSLTCETQSYHQAAAAYH 180

Query: 181 ESSIHPFLYPQSSSSRPPCSDFSPINTNPQLFLYSGTHAQAGKDYSRDMDGPREGVGVQS 240
           ESSIHPFLYPQSSSSRPPCSDFSPINTNPQLFLYSGT AQAGKDYSRDMDGPREGVGVQS
Sbjct: 181 ESSIHPFLYPQSSSSRPPCSDFSPINTNPQLFLYSGT-AQAGKDYSRDMDGPREGVGVQS 240

Query: 241 FFPSIADSARSLAEVYNQPMAMDSYKQSVSQSHQIYSSKQARHQQEQHCFVLGTDFNKPS 300
           FFPSIADSARSLAEVYNQPMAMDSYKQSVSQSHQIYSSKQARHQQEQHCFVLGTDFNKPS
Sbjct: 241 FFPSIADSARSLAEVYNQPMAMDSYKQSVSQSHQIYSSKQARHQQEQHCFVLGTDFNKPS 300

Query: 301 RSAKTEEEEEEDAETQKPLLHFFEDCPPKNSDSWLDLASSSSIQSGNFIGL 352
           RSAKTEEEEEEDAETQKPLLHFFEDCPPKNSDSWLDLASSSSIQSGNFIGL
Sbjct: 301 RSAKTEEEEEEDAETQKPLLHFFEDCPPKNSDSWLDLASSSSIQSGNFIGL 350

BLAST of MS016193 vs. ExPASy TrEMBL
Match: A0A6J1DEN2 (Growth-regulating factor OS=Momordica charantia OX=3673 GN=LOC111020348 PE=3 SV=1)

HSP 1 Score: 683.7 bits (1763), Expect = 4.0e-193
Identity = 349/351 (99.43%), Postives = 349/351 (99.43%), Query Frame = 0

Query: 1   MNSSPKACTSPFTATQWQELEHQALIFKYMVSGVPIPPDLIFSVKRSLDSSISARLFPHQ 60
           MNSSPKACTSPFTATQWQELEHQALIFKYMVSGVPIPPDLIFSVKRSLDSSISARLFPHQ
Sbjct: 1   MNSSPKACTSPFTATQWQELEHQALIFKYMVSGVPIPPDLIFSVKRSLDSSISARLFPHQ 60

Query: 61  PIGWGCFEMGFGRKADPEPGRCRRTDGKKWRCSKEAYPDSKYCERHMHRGRNRSRKPVEI 120
           PIGWGCFEMGFGRKADPEPGRCRRTDGKKWRCSKEAYPDSKYCERHMHRGRNRSRKPVEI
Sbjct: 61  PIGWGCFEMGFGRKADPEPGRCRRTDGKKWRCSKEAYPDSKYCERHMHRGRNRSRKPVEI 120

Query: 121 SSTPSTQTQPPPPLILTSNRSLSSSSTPTTSSYSLSTLSSSSSSLTCETQSYHQAAAAYH 180
           SSTPSTQTQPPPPLILTSNRSLSSSSTPTTSSYSLSTLSSSSSSLTCETQSYHQAAAAYH
Sbjct: 121 SSTPSTQTQPPPPLILTSNRSLSSSSTPTTSSYSLSTLSSSSSSLTCETQSYHQAAAAYH 180

Query: 181 ESSIHPFLYPQSSSSRPPCSDFSPINTNPQLFLYSGTHAQAGKDYSRDMDGPREGVGVQS 240
           ESSIHPFLYPQSSSSRPPCSDFSPINTNPQLFLYSGT AQAGKDY RDMDGPREGVGVQS
Sbjct: 181 ESSIHPFLYPQSSSSRPPCSDFSPINTNPQLFLYSGT-AQAGKDY-RDMDGPREGVGVQS 240

Query: 241 FFPSIADSARSLAEVYNQPMAMDSYKQSVSQSHQIYSSKQARHQQEQHCFVLGTDFNKPS 300
           FFPSIADSARSLAEVYNQPMAMDSYKQSVSQSHQIYSSKQARHQQEQHCFVLGTDFNKPS
Sbjct: 241 FFPSIADSARSLAEVYNQPMAMDSYKQSVSQSHQIYSSKQARHQQEQHCFVLGTDFNKPS 300

Query: 301 RSAKTEEEEEEDAETQKPLLHFFEDCPPKNSDSWLDLASSSSIQSGNFIGL 352
           RSAKTEEEEEEDAETQKPLLHFFEDCPPKNSDSWLDLASSSSIQSGNFIGL
Sbjct: 301 RSAKTEEEEEEDAETQKPLLHFFEDCPPKNSDSWLDLASSSSIQSGNFIGL 349

BLAST of MS016193 vs. ExPASy TrEMBL
Match: A0A6J1DIH7 (Growth-regulating factor OS=Momordica charantia OX=3673 GN=LOC111020348 PE=3 SV=1)

HSP 1 Score: 671.4 bits (1731), Expect = 2.1e-189
Identity = 340/342 (99.42%), Postives = 341/342 (99.71%), Query Frame = 0

Query: 1   MNSSPKACTSPFTATQWQELEHQALIFKYMVSGVPIPPDLIFSVKRSLDSSISARLFPHQ 60
           MNSSPKACTSPFTATQWQELEHQALIFKYMVSGVPIPPDLIFSVKRSLDSSISARLFPHQ
Sbjct: 1   MNSSPKACTSPFTATQWQELEHQALIFKYMVSGVPIPPDLIFSVKRSLDSSISARLFPHQ 60

Query: 61  PIGWGCFEMGFGRKADPEPGRCRRTDGKKWRCSKEAYPDSKYCERHMHRGRNRSRKPVEI 120
           PIGWGCFEMGFGRKADPEPGRCRRTDGKKWRCSKEAYPDSKYCERHMHRGRNRSRKPVEI
Sbjct: 61  PIGWGCFEMGFGRKADPEPGRCRRTDGKKWRCSKEAYPDSKYCERHMHRGRNRSRKPVEI 120

Query: 121 SSTPSTQTQPPPPLILTSNRSLSSSSTPTTSSYSLSTLSSSSSSLTCETQSYHQAAAAYH 180
           SSTPSTQTQPPPPLILTSNRSLSSSSTPTTSSYSLSTLSSSSSSLTCETQSYHQAAAAYH
Sbjct: 121 SSTPSTQTQPPPPLILTSNRSLSSSSTPTTSSYSLSTLSSSSSSLTCETQSYHQAAAAYH 180

Query: 181 ESSIHPFLYPQSSSSRPPCSDFSPINTNPQLFLYSGTHAQAGKDYSRDMDGPREGVGVQS 240
           ESSIHPFLYPQSSSSRPPCSDFSPINTNPQLFLYSGT AQAGKDYSRDMDGPREGVGVQS
Sbjct: 181 ESSIHPFLYPQSSSSRPPCSDFSPINTNPQLFLYSGT-AQAGKDYSRDMDGPREGVGVQS 240

Query: 241 FFPSIADSARSLAEVYNQPMAMDSYKQSVSQSHQIYSSKQARHQQEQHCFVLGTDFNKPS 300
           FFPSIADSARSLAEVYNQPMAMDSYKQSVSQSHQIYSSKQARHQQEQHCFVLGTDFNKPS
Sbjct: 241 FFPSIADSARSLAEVYNQPMAMDSYKQSVSQSHQIYSSKQARHQQEQHCFVLGTDFNKPS 300

Query: 301 RSAKTEEEEEEDAETQKPLLHFFEDCPPKNSDSWLDLASSSS 343
           RSAKTEEEEEEDAETQKPLLHFFEDCPPKNSDSWLDLASSS+
Sbjct: 301 RSAKTEEEEEEDAETQKPLLHFFEDCPPKNSDSWLDLASSSN 341

BLAST of MS016193 vs. ExPASy TrEMBL
Match: A0A6J1G9Z5 (Growth-regulating factor OS=Cucurbita moschata OX=3662 GN=LOC111452139 PE=3 SV=1)

HSP 1 Score: 590.1 bits (1520), Expect = 6.0e-165
Identity = 310/362 (85.64%), Postives = 326/362 (90.06%), Query Frame = 0

Query: 1   MNSSPKACTSPFTATQWQELEHQALIFKYMVSGVPIPPDLIFSVKRSLDSSISARLFPHQ 60
           MNS PK C SPFT TQWQELEHQALIFKYMVSGVPIPPDLIFSVKRSLDSSISARLFPHQ
Sbjct: 1   MNSGPKPCVSPFTPTQWQELEHQALIFKYMVSGVPIPPDLIFSVKRSLDSSISARLFPHQ 60

Query: 61  PIGWGCFEMGFGRKADPEPGRCRRTDGKKWRCSKEAYPDSKYCERHMHRGRNRSRKPVEI 120
           PIGWGCFEMGFGRKADPEPGRCRRTDGKKWRCSKEAYPDSKYCERHMHRGRNRSRKPVEI
Sbjct: 61  PIGWGCFEMGFGRKADPEPGRCRRTDGKKWRCSKEAYPDSKYCERHMHRGRNRSRKPVEI 120

Query: 121 SSTPSTQTQPPPPLILTSNRSLSSSSTPTTSSYSLSTLSSSSSSLTCETQSYHQ------ 180
           SSTP  QT  PPPLILTS RSLS+SS PT+SSYSLSTL SSSSSLTCETQSYHQ      
Sbjct: 121 SSTPPAQT--PPPLILTSTRSLSASS-PTSSSYSLSTL-SSSSSLTCETQSYHQAPAPAP 180

Query: 181 --AAAAYHESSIHPFLYPQSSSSRPPCSDFSPINTNPQLFLYSGTHAQAGKDYSRDMDGP 240
             A AAY ESSIHP+LYPQSSSSRPPCSDFSP +TNPQLFLYSGTH QA KDY RD++GP
Sbjct: 181 APAPAAYQESSIHPYLYPQSSSSRPPCSDFSPNSTNPQLFLYSGTHPQADKDY-RDINGP 240

Query: 241 REGVGVQSFFPSIADSARSLAEVYNQPMAMDSYK---QSVSQSHQIYSSKQARHQQEQHC 300
           REGVGVQ+FFPS+ADSARS+ EVY+QP+ MDSYK    +VS SHQIYSSKQAR QQEQHC
Sbjct: 241 REGVGVQAFFPSVADSARSIGEVYSQPIGMDSYKGFSHAVSNSHQIYSSKQARQQQEQHC 300

Query: 301 FVLGTDFNKPSRSAKTEEEEEEDAETQKPLLHFFEDCPPKNSDSWLDLASSSSIQSGNFI 352
           FVLGTDFNK +RSAKT  EEE+DAETQKPLLHFFEDCPPKNSDSWLDLA++SSIQSGNFI
Sbjct: 301 FVLGTDFNKSTRSAKT--EEEDDAETQKPLLHFFEDCPPKNSDSWLDLATNSSIQSGNFI 355

BLAST of MS016193 vs. ExPASy TrEMBL
Match: A0A6J1KHP6 (Growth-regulating factor OS=Cucurbita maxima OX=3661 GN=LOC111493342 PE=3 SV=1)

HSP 1 Score: 585.5 bits (1508), Expect = 1.5e-163
Identity = 307/359 (85.52%), Postives = 325/359 (90.53%), Query Frame = 0

Query: 1   MNSSPKACTSPFTATQWQELEHQALIFKYMVSGVPIPPDLIFSVKRSLDSSISARLFPHQ 60
           MNS PK C SPFT TQWQELEHQALIFKYMVSGVPIPPDLIFSVKRSLDSSISARLFPHQ
Sbjct: 1   MNSGPKPCISPFTPTQWQELEHQALIFKYMVSGVPIPPDLIFSVKRSLDSSISARLFPHQ 60

Query: 61  PIGWGCFEMGFGRKADPEPGRCRRTDGKKWRCSKEAYPDSKYCERHMHRGRNRSRKPVEI 120
           PIGWGCFEMGFGRKADPEPGRCRRTDGKKWRCSKEAYPDSKYCERHMHRGRNRSRKPVEI
Sbjct: 61  PIGWGCFEMGFGRKADPEPGRCRRTDGKKWRCSKEAYPDSKYCERHMHRGRNRSRKPVEI 120

Query: 121 SSTPSTQTQPPPPLILTSNRSLSSSSTPTTSSYSLSTLSSSSSSLTCETQSYHQA----- 180
           SSTP  QT  PPPLILTS RSLS+SS PT+SSYSL+TL SSSSSLTCETQSY QA     
Sbjct: 121 SSTPPAQT--PPPLILTSTRSLSASS-PTSSSYSLATL-SSSSSLTCETQSYQQAPAPAP 180

Query: 181 AAAYHESSIHPFLYPQSSSSRPPCSDFSPINTNPQLFLYSGTHAQAGKDYSRDMDGPREG 240
           AAAY ESSIHP+LYPQSSSSRPPCSDFSP +TNPQLFLYSGTH QA KDY RD++GPREG
Sbjct: 181 AAAYQESSIHPYLYPQSSSSRPPCSDFSPTSTNPQLFLYSGTHPQADKDY-RDINGPREG 240

Query: 241 VGVQSFFPSIADSARSLAEVYNQPMAMDSYK---QSVSQSHQIYSSKQARHQQEQHCFVL 300
            GVQ+FFPS+ADSARS+ EVY+QP+ MDSYK    +VS S+QIYS+KQAR QQEQHCFVL
Sbjct: 241 AGVQAFFPSVADSARSIGEVYSQPIGMDSYKGFSHAVSNSYQIYSAKQARQQQEQHCFVL 300

Query: 301 GTDFNKPSRSAKTEEEEEEDAETQKPLLHFFEDCPPKNSDSWLDLASSSSIQSGNFIGL 352
           GTDFNK SRSAKT  EEE+DAETQKPLLHFFEDCPPKNSDSWLDLA++SSIQSGNFIGL
Sbjct: 301 GTDFNKSSRSAKT--EEEDDAETQKPLLHFFEDCPPKNSDSWLDLATNSSIQSGNFIGL 352

BLAST of MS016193 vs. TAIR 10
Match: AT3G13960.1 (growth-regulating factor 5 )

HSP 1 Score: 249.2 bits (635), Expect = 4.9e-66
Identity = 167/385 (43.38%), Postives = 225/385 (58.44%), Query Frame = 0

Query: 11  PFTATQWQELEHQALIFKYMVSGVPIPPDLIFSVKRSLDSSISARLFPHQPIGWGCFEMG 70
           PFT TQW+ELEHQALI+KYMVSGVP+PP+LIFS++RSLD+S+ +RL PHQ +GWGC++MG
Sbjct: 16  PFTPTQWEELEHQALIYKYMVSGVPVPPELIFSIRRSLDTSLVSRLLPHQSLGWGCYQMG 75

Query: 71  FGRKADPEPGRCRRTDGKKWRCSKEAYPDSKYCERHMHRGRNRSRKPVEISSTPSTQTQP 130
           FGRK DPEPGRCRRTDGKKWRCS+EAYPDSKYCE+HMHRGRNR+RK ++ + T +T    
Sbjct: 76  FGRKPDPEPGRCRRTDGKKWRCSREAYPDSKYCEKHMHRGRNRARKSLDQNQTTTTPLTS 135

Query: 131 PPPLILTSNRSLSSSSTPTTSSYSLSTLSSSSSSLTCETQSYHQAAAAYHES-----SIH 190
           P      +N    + S+ ++S+ S +T S+SSSS+   + S          +     + H
Sbjct: 136 PSLSFTNNNNPSPTLSSSSSSNSSSTTYSASSSSMDAYSNSNRFGLGGSSSNTRGYFNSH 195

Query: 191 PFLYPQSSSSRPPCSDFSPINTNPQLFLYSGTH----------AQAGKDYSRDMDGPRE- 250
              YP  S+S  P      ++    L L+  T+          A   KD+ R   G  E 
Sbjct: 196 SLDYPYPSTS--PKQQQQTLHHASALSLHQNTNSTSQFNVLASATDHKDF-RYFQGIGER 255

Query: 251 --GVGVQSFFP----SIADS-----ARSLAEVYNQPMAMDSYKQSVSQSHQIYSSKQA-- 310
             GVG ++FFP    S  DS      + LA V N P    S   +    H  YSS  +  
Sbjct: 256 VGGVGERTFFPEASRSFQDSPYHHHQQPLATVMNDPYHHCSTDHNKIDHHHTYSSSSSSQ 315

Query: 311 -----RHQQEQHCFVLGTD-FNKPSRSA--------KTEEEEEEDA--ETQKPLLHFFED 348
                   ++Q CFVLG D FNKP+RS         + +EE+E+D+   ++K L HFF +
Sbjct: 316 HLHHDHDHRQQQCFVLGADMFNKPTRSVLANSSRQDQNQEEDEKDSSESSKKSLHHFFGE 375

BLAST of MS016193 vs. TAIR 10
Match: AT2G06200.1 (growth-regulating factor 6 )

HSP 1 Score: 151.8 bits (382), Expect = 1.1e-36
Identity = 86/170 (50.59%), Postives = 103/170 (60.59%), Query Frame = 0

Query: 11  PFTATQWQELEHQALIFKYMVSGVPIPPDLIFSVKR-----------SLDSSISARLFPH 70
           PFT +QW+ELE+QAL+FKY+ + +P+PP L+F +KR           S  S  S  L PH
Sbjct: 6   PFTESQWEELENQALVFKYLAANMPVPPHLLFLIKRPFLFSSSSSSSSSSSFFSPTLSPH 65

Query: 71  QPIGWGCFEMGFGRKADPEPGRCRRTDGKKWRCSKEAYPDSKYCERHMHRGRNRSRKPVE 130
              GW  +EMG GRK D EPGRCRRTDGKKWRCSKEAYPDSKYCERHMHRG+NRS     
Sbjct: 66  --FGWNVYEMGMGRKIDAEPGRCRRTDGKKWRCSKEAYPDSKYCERHMHRGKNRS----- 125

Query: 131 ISSTPSTQTQPPPPLILTSNRSLSSSSTPTTSSY--SLSTLSSSSSSLTC 168
                   ++ PPP   T N  L SSS    S Y     ++  S S  +C
Sbjct: 126 -------SSRKPPPTQFTPNLFLDSSSRRRRSGYMDDFFSIEPSGSIKSC 161

BLAST of MS016193 vs. TAIR 10
Match: AT4G37740.1 (growth-regulating factor 2 )

HSP 1 Score: 136.3 bits (342), Expect = 4.6e-32
Identity = 91/210 (43.33%), Postives = 118/210 (56.19%), Query Frame = 0

Query: 11  PFTATQWQELEHQALIFKYMVSGVPIPPDLIFSVKRSLDSSISARLFPHQPIGWGCFEMG 70
           PFT TQW ELE QALI+KY+ + VP+P  L+ S+K+S     S    P    GWG F +G
Sbjct: 164 PFTLTQWAELEQQALIYKYITANVPVPSSLLISIKKSFYPYGS---LPPSSFGWGTFHLG 223

Query: 71  F-GRKADPEPGRCRRTDGKKWRCSKEAYPDSKYCERHMHRGRNRSRKPVEISS------- 130
           F G   DPEPGRCRRTDGKKWRCS++A PD KYCERH++RGR+RSRKPVE+ S       
Sbjct: 224 FAGGNMDPEPGRCRRTDGKKWRCSRDAVPDQKYCERHINRGRHRSRKPVEVQSGQNQTAA 283

Query: 131 -TPSTQTQPPPPLI----------LTSNRSLSSSS---TPTTSSY----SLSTLSSSSSS 190
                 T P  P++           +SNRSL+  S    P+T S      +S   S+ + 
Sbjct: 284 AASKAVTTPQQPVVAGNTNRSNARASSNRSLAIGSQYINPSTESLPNNRGVSIYPSTVNL 343

Query: 191 LTCETQSYHQAAAAYHESSIHPFLYPQSSS 195
              E+   HQ     H ++ +PF +   SS
Sbjct: 344 QPKESPVIHQK----HRNNNNPFEFGHISS 366

BLAST of MS016193 vs. TAIR 10
Match: AT2G22840.1 (growth-regulating factor 1 )

HSP 1 Score: 130.6 bits (327), Expect = 2.5e-30
Identity = 81/201 (40.30%), Postives = 113/201 (56.22%), Query Frame = 0

Query: 11  PFTATQWQELEHQALIFKYMVSGVPIPPDLIFSVKRSLDSSISARLFPHQPIGWGCFEMG 70
           PF+ TQW ELE QALI+KY+ + VP+P  L+ S+K+S     S    P    GWG F +G
Sbjct: 133 PFSLTQWAELEQQALIYKYITANVPVPSSLLLSLKKSFFPYGS---LPPNSFGWGSFHLG 192

Query: 71  F-GRKADPEPGRCRRTDGKKWRCSKEAYPDSKYCERHMHRGRNRSRKPVEISSTPSTQTQ 130
           F G   DPEPGRCRRTDGKKWRCS++A PD KYCERH++RGR+RSRKPVE  +  +T   
Sbjct: 193 FSGGNMDPEPGRCRRTDGKKWRCSRDAVPDQKYCERHINRGRHRSRKPVEGQNGHNTNAA 252

Query: 131 PPPPLILTSNRSLSSSSTPTTSSYSLSTLSSSSSSLTCETQSYHQAAAAYHESSIHPFLY 190
                   S  +  S +   TS+ ++   S +++SL     + H       +S  +    
Sbjct: 253 AAASAAAASTAAAVSKAAAGTSAVAMRG-SDNNNSLAAAVGTQHHTNNQSTDSLANRVQN 312

Query: 191 PQSSSSRPPCSDFSPINTNPQ 211
            + +S  P   +     T+P+
Sbjct: 313 SRGASVFPATMNLQSKETHPK 329

BLAST of MS016193 vs. TAIR 10
Match: AT2G36400.1 (growth-regulating factor 3 )

HSP 1 Score: 122.1 bits (305), Expect = 9.0e-28
Identity = 70/152 (46.05%), Postives = 96/152 (63.16%), Query Frame = 0

Query: 12  FTATQWQELEHQALIFKYMVSGVPIPPDLIFSVKRSLDSSISARLFPHQPI--------G 71
           F+  QWQELE QALI++YM++G  +P +L+  +K+SL   +S   F H P+         
Sbjct: 77  FSWAQWQELELQALIYRYMLAGAAVPQELLLPIKKSL-LHLSPSYFLHHPLQHLPHYQPA 136

Query: 72  WGCFEMGFGRKA-DPEPGRCRRTDGKKWRCSKEAYPDSKYCERHMHRGRNRSRKPVEISS 131
           W       GR A DPEPGRCRRTDGKKWRCS++ +   KYCERHMHRGRNRSRKPVE   
Sbjct: 137 W-----YLGRAAMDPEPGRCRRTDGKKWRCSRDVFAGHKYCERHMHRGRNRSRKPVE--- 196

Query: 132 TPSTQTQPPPPLILTSNRSLSSSSTPTTSSYS 155
           TP+T       +  +   + ++++  TTS+++
Sbjct: 197 TPTTVNATATSMASSVAAAATTTTATTTSTFA 219

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022152689.11.2e-19499.72growth-regulating factor 5-like isoform X1 [Momordica charantia][more]
XP_022152690.18.3e-19399.43growth-regulating factor 5-like isoform X2 [Momordica charantia][more]
XP_022152691.14.3e-18999.42growth-regulating factor 5-like isoform X3 [Momordica charantia][more]
XP_038879715.11.5e-16588.17growth-regulating factor 5 isoform X1 [Benincasa hispida][more]
XP_023525289.13.3e-16587.08growth-regulating factor 5-like isoform X1 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q8L8A66.8e-6543.38Growth-regulating factor 5 OS=Arabidopsis thaliana OX=3702 GN=GRF5 PE=1 SV=1[more]
A2XA734.6e-5341.87Growth-regulating factor 1 OS=Oryza sativa subsp. indica OX=39946 GN=GRF1 PE=2 S... [more]
Q6AWY83.0e-5241.60Growth-regulating factor 1 OS=Oryza sativa subsp. japonica OX=39947 GN=GRF1 PE=3... [more]
A0A060D7649.9e-4058.87Growth-regulating factor 1 OS=Zea mays OX=4577 GN=GRF1 PE=1 SV=1[more]
Q6ZIK56.4e-3937.13Growth-regulating factor 4 OS=Oryza sativa subsp. japonica OX=39947 GN=GRF4 PE=1... [more]
Match NameE-valueIdentityDescription
A0A6J1DGS75.6e-19599.72Growth-regulating factor OS=Momordica charantia OX=3673 GN=LOC111020348 PE=3 SV=... [more]
A0A6J1DEN24.0e-19399.43Growth-regulating factor OS=Momordica charantia OX=3673 GN=LOC111020348 PE=3 SV=... [more]
A0A6J1DIH72.1e-18999.42Growth-regulating factor OS=Momordica charantia OX=3673 GN=LOC111020348 PE=3 SV=... [more]
A0A6J1G9Z56.0e-16585.64Growth-regulating factor OS=Cucurbita moschata OX=3662 GN=LOC111452139 PE=3 SV=1[more]
A0A6J1KHP61.5e-16385.52Growth-regulating factor OS=Cucurbita maxima OX=3661 GN=LOC111493342 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G13960.14.9e-6643.38growth-regulating factor 5 [more]
AT2G06200.11.1e-3650.59growth-regulating factor 6 [more]
AT4G37740.14.6e-3243.33growth-regulating factor 2 [more]
AT2G22840.12.5e-3040.30growth-regulating factor 1 [more]
AT2G36400.19.0e-2846.05growth-regulating factor 3 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR014978Glutamine-Leucine-Glutamine, QLQSMARTSM00951QLQ_2coord: 10..46
e-value: 1.1E-12
score: 58.2
IPR014978Glutamine-Leucine-Glutamine, QLQPFAMPF08880QLQcoord: 11..44
e-value: 1.1E-15
score: 57.0
IPR014978Glutamine-Leucine-Glutamine, QLQPROSITEPS51666QLQcoord: 11..46
score: 22.394411
IPR014977WRC domainPFAMPF08879WRCcoord: 77..119
e-value: 1.9E-21
score: 75.5
IPR014977WRC domainPROSITEPS51667WRCcoord: 76..120
score: 24.94142
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 295..317
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 103..155
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 132..155
NoneNo IPR availablePANTHERPTHR31602:SF60GROWTH-REGULATING FACTOR 5coord: 9..345
IPR031137Growth-regulating factorPANTHERPTHR31602FAMILY NOT NAMEDcoord: 9..345

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS016193.1MS016193.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032502 developmental process
biological_process GO:0006355 regulation of transcription, DNA-templated
biological_process GO:0006351 transcription, DNA-templated
cellular_component GO:0005634 nucleus
molecular_function GO:0005524 ATP binding