MS016191 (gene) Bitter gourd (TR) v1

Overview
NameMS016191
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionGTP diphosphokinase
Locationscaffold9_2: 986274 .. 989222 (+)
RNA-Seq ExpressionMS016191
SyntenyMS016191
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTGTGCCAACCATAGCTCTCTATACGAGCCCACCGAGCACCATCTGCTCCTCACCGCACCCTTGCCACATGAATTCTCACGCATCATACGATTTGGAATTTACTTCTCGGTCCTCGTCGCTGCCATCTTCCACGGCCTCTTCGTCCCAGAAACCGATGATTGGGGGGTTGTCGAGTTTGTTCTCGTCGACAACGGCGAGACACACCTCATCGTCGGCGAGCATTTCAAGTGGTGGGGATGAGCTAGGTTCTTTTAGGCACGATAAAGGGGAGGAACTTAAAGAATTGAGTTCCTCGTTTCGTTATTCCCCTAGTAAATTCATTGGGTCCTTCTTCAATCGGGATCAGAGCCCAGTATCAGTGTTCCAGGGTCCGGTTTCCTGTGGCAGTTGTGGGGTCGGGTCGGCGGTGAGAACCCCTCCCTTGTGGACTGGAAGGGAAAGGAGTGGGGATGCTAGTTTTCATGGACGAGGAAGCTCCAACAGGCTGTTCAATGGGTTTGTGAGAAATGCATTGGGATCGTGTGTGGACTATGATTCACCGAGATTGGAGGTGTCTAGTGATTGTTTAGATGTGGGTTCGTCAGCGTTGATTGTTGATGAGTTGACTTTCAACATGGAGGACAATATTGCAGAAGGTAATTCCGAGTCATATGCAAAAGATTTGCTCCTAAGTGCCCAATCGAAGCACAAAATCTTTTGTGACGAGTTTGTGATTAAGGCTTTTTTTGAGGCCGAAAAAGCACATAGAGGACAGGTAATGCAGTCTTTTTATATCGACAGTGTTGTTATTGCCTTGAGTACTGATGACATCCATTAAGAAACTTTTTTTTTTGGTCTGTTTTTTGTCTTCCTTAGATGCGTGCAAGTGGCGATCCATACTTCGAGCATTGTGTGGAAACAGCGGTGATGCTTGCGCTTGTTGGTGCTAATTCCACAGTCGTTGCTGCAGGACTCTTGCACGACACACTTGATGATTCTTTTGTGAGTCATGACTACATATTGGGGACGTTCGGAGCTGGGGTTGCTGATTTAGTTGAAGGGGTAAGATACCGCTGTAGTTATAATTGTGCAATATATCCATTAACAAGTGATGCCGCACACACTTCCCTTGTCATTGGAAGAGGTGGCTTAGGCTTAGGCATAGGACATAATTGCAAAATTTAATCTATTTTCTTGTGAGAAAGAAAATTGCAGATTGCAATAGTGTTAAATTTGGGGTGAAAAGTAACATTCATGCTGTAGGTCTCTTATTGGACAACTCTCTTGCACTTTCATTTTCTTATACATGTTTTTGCATTTTTTCTAGAATTATTGTTCTGAATGGGTTACTTATCTAGCTTCCTTTTTTGTTTTTTCTTTATAATTTTACTGTGTTTAGGTGTCTAAGCTAAGTCATTTAAGCAAGCTTGCTCGTGAACATGATACGGCTGATAGAATGGTTGAGGCAGACCGCTTGCACACCATGTTCCTCGCTATGGCTGATGCAAGAGCGGTTCTCATTAAATTAGCAGACCGTTTGCACAATATGATGACTTTGGATGCATTGCCTCTGATCAAGCGGCAAAGGTTTGCCAAGGAGACTATGGAGATATTTGTTCCTTTGGCGAATCGCTTAGGAATCTACAGTTGGAAGGAGCAGCTAGAAAACTTGTGTTTTAAGCATCTTAACCTGGAACAGCACAAAGATTTGTCATCCAAACTTATGGGTTTATATGATGAAGCAATTATATATTCTGCAATTCAGAAATTAGAGCGAGCTCTCGAGGATAAAGGAATCTCTTATCATGTTGTAACTGGGCGGCACAAAAGTGTCTACAGCGTTCACTGCAAAATGTTGAAGTATGCGTTCTGGCTTCCATCTCTCTCTCTTGTTTTCCCTTCATCTCTGCAGACTTGTTTTCTGACATCTTGTGTTTGGAAGAGTGATTTTTAGTTGAATCTGAATTTGTTTTGCAGGAAGAATTTGACAATGAATGAGATCCATGATATTCATGGATTGAGGCTCATTGTTGAAAAGGAAGAGGATTGCTATGAAGCTTTGAGAATCGTTCATCAGTTATGGCCCGAAGTACCGGGAAAGCTCAAGGACTACATAAGTAAACCGAAGTTAAATGGGTATTTCTCACACTGCAGTTTCTATTGCTTTATTTCCTATATATTTCTTCTCTTATATAATCTGATGGTAAACAGGTATCAATCTATACACACAGTAGTAAGGGGTGAAGGTGACATCCCACTCGAGGTTCAAATTCGGACCAAGAAGATGCATTTGCAAGCCGAGTTCGGGTTTGCTGCACATTGGAGATACAAAGAGGGTGATTCTAAGCACTCTTCATTTGTGCTTCAGATGGTTGAATGGGCCAGATGGGTTCTCACATGGCATTGTGAGACTATGAGCAAAGATCGATCTTCGATCACCTACGCCGATTCTGTCAAACCACCCTGCAAGTTCCCTTTTCACTCTTCTGACTGCTCGTACTCTTACAAACCTCACTGCGTCCAGGATGGGCCTTTGTTTGTCATTATGATTGAAAATGAAAAGGTCTATGCTTATTATTATTCTAGTTAATTCTGTTAAAAGTTAGTTATCTGTCTTGAGTGCGAAAGTGCGGATTTAGCAATTCAGAAAATACTCTGTTTTGGTTATCTCCAGATGTCGGTCCAGGAATTCCCGGCTAATGCAACGATGATGGATCTTTTAGAAAGAGCGGGGAGAGGAAGCACGAGATGGACTCACTACAGGTTCCCAATGAAGGAAGAGTTGAGACCAAGGCTGAACCACAAGCCCGTGAGCGACCCCAAGTGCAAGCTGAAGATGGGCGATGTGGTGGAATTGACCCCGCCGATACCAGACAAGTCGTTGATCGAGTACCGGGAAGAAATCCAGCGAATGTATGAGGGAGGATTTACTGTTGCAACTCCAGAACCTGCAGGTTGGAGTAGC

mRNA sequence

ATGGCTGTGCCAACCATAGCTCTCTATACGAGCCCACCGAGCACCATCTGCTCCTCACCGCACCCTTGCCACATGAATTCTCACGCATCATACGATTTGGAATTTACTTCTCGGTCCTCGTCGCTGCCATCTTCCACGGCCTCTTCGTCCCAGAAACCGATGATTGGGGGGTTGTCGAGTTTGTTCTCGTCGACAACGGCGAGACACACCTCATCGTCGGCGAGCATTTCAAGTGGTGGGGATGAGCTAGGTTCTTTTAGGCACGATAAAGGGGAGGAACTTAAAGAATTGAGTTCCTCGTTTCGTTATTCCCCTAGTAAATTCATTGGGTCCTTCTTCAATCGGGATCAGAGCCCAGTATCAGTGTTCCAGGGTCCGGTTTCCTGTGGCAGTTGTGGGGTCGGGTCGGCGGTGAGAACCCCTCCCTTGTGGACTGGAAGGGAAAGGAGTGGGGATGCTAGTTTTCATGGACGAGGAAGCTCCAACAGGCTGTTCAATGGGTTTGTGAGAAATGCATTGGGATCGTGTGTGGACTATGATTCACCGAGATTGGAGGTGTCTAGTGATTGTTTAGATGTGGGTTCGTCAGCGTTGATTGTTGATGAGTTGACTTTCAACATGGAGGACAATATTGCAGAAGGTAATTCCGAGTCATATGCAAAAGATTTGCTCCTAAGTGCCCAATCGAAGCACAAAATCTTTTGTGACGAGTTTGTGATTAAGGCTTTTTTTGAGGCCGAAAAAGCACATAGAGGACAGATGCGTGCAAGTGGCGATCCATACTTCGAGCATTGTGTGGAAACAGCGGTGATGCTTGCGCTTGTTGGTGCTAATTCCACAGTCGTTGCTGCAGGACTCTTGCACGACACACTTGATGATTCTTTTGTGAGTCATGACTACATATTGGGGACGTTCGGAGCTGGGGTTGCTGATTTAGTTGAAGGGGTGTCTAAGCTAAGTCATTTAAGCAAGCTTGCTCGTGAACATGATACGGCTGATAGAATGGTTGAGGCAGACCGCTTGCACACCATGTTCCTCGCTATGGCTGATGCAAGAGCGGTTCTCATTAAATTAGCAGACCGTTTGCACAATATGATGACTTTGGATGCATTGCCTCTGATCAAGCGGCAAAGGTTTGCCAAGGAGACTATGGAGATATTTGTTCCTTTGGCGAATCGCTTAGGAATCTACAGTTGGAAGGAGCAGCTAGAAAACTTGTGTTTTAAGCATCTTAACCTGGAACAGCACAAAGATTTGTCATCCAAACTTATGGGTTTATATGATGAAGCAATTATATATTCTGCAATTCAGAAATTAGAGCGAGCTCTCGAGGATAAAGGAATCTCTTATCATGTTGTAACTGGGCGGCACAAAAGTGTCTACAGCGTTCACTGCAAAATGTTGAAGAAGAATTTGACAATGAATGAGATCCATGATATTCATGGATTGAGGCTCATTGTTGAAAAGGAAGAGGATTGCTATGAAGCTTTGAGAATCGTTCATCAGTTATGGCCCGAAGTACCGGGAAAGCTCAAGGACTACATAAGTAAACCGAAGTTAAATGGGTATTTCTCACACTGCAGTTTCTATTGCTTTATTTCCTATATATTTCTTCTCTTATATAATCTGATGGTAAACAGGTATCAATCTATACACACAGTAGTAAGGGGTGAAGGTGACATCCCACTCGAGGTTCAAATTCGGACCAAGAAGATGCATTTGCAAGCCGAGTTCGGGTTTGCTGCACATTGGAGATACAAAGAGGGTGATTCTAAGCACTCTTCATTTGTGCTTCAGATGGTTGAATGGGCCAGATGGGTTCTCACATGGCATTGTGAGACTATGAGCAAAGATCGATCTTCGATCACCTACGCCGATTCTGTCAAACCACCCTGCAAGTTCCCTTTTCACTCTTCTGACTGCTCGTACTCTTACAAACCTCACTGCGTCCAGGATGGGCCTTTGTTTGTCATTATGATTGAAAATGAAAAGATGTCGGTCCAGGAATTCCCGGCTAATGCAACGATGATGGATCTTTTAGAAAGAGCGGGGAGAGGAAGCACGAGATGGACTCACTACAGGTTCCCAATGAAGGAAGAGTTGAGACCAAGGCTGAACCACAAGCCCGTGAGCGACCCCAAGTGCAAGCTGAAGATGGGCGATGTGGTGGAATTGACCCCGCCGATACCAGACAAGTCGTTGATCGAGTACCGGGAAGAAATCCAGCGAATGTATGAGGGAGGATTTACTGTTGCAACTCCAGAACCTGCAGGTTGGAGTAGC

Coding sequence (CDS)

ATGGCTGTGCCAACCATAGCTCTCTATACGAGCCCACCGAGCACCATCTGCTCCTCACCGCACCCTTGCCACATGAATTCTCACGCATCATACGATTTGGAATTTACTTCTCGGTCCTCGTCGCTGCCATCTTCCACGGCCTCTTCGTCCCAGAAACCGATGATTGGGGGGTTGTCGAGTTTGTTCTCGTCGACAACGGCGAGACACACCTCATCGTCGGCGAGCATTTCAAGTGGTGGGGATGAGCTAGGTTCTTTTAGGCACGATAAAGGGGAGGAACTTAAAGAATTGAGTTCCTCGTTTCGTTATTCCCCTAGTAAATTCATTGGGTCCTTCTTCAATCGGGATCAGAGCCCAGTATCAGTGTTCCAGGGTCCGGTTTCCTGTGGCAGTTGTGGGGTCGGGTCGGCGGTGAGAACCCCTCCCTTGTGGACTGGAAGGGAAAGGAGTGGGGATGCTAGTTTTCATGGACGAGGAAGCTCCAACAGGCTGTTCAATGGGTTTGTGAGAAATGCATTGGGATCGTGTGTGGACTATGATTCACCGAGATTGGAGGTGTCTAGTGATTGTTTAGATGTGGGTTCGTCAGCGTTGATTGTTGATGAGTTGACTTTCAACATGGAGGACAATATTGCAGAAGGTAATTCCGAGTCATATGCAAAAGATTTGCTCCTAAGTGCCCAATCGAAGCACAAAATCTTTTGTGACGAGTTTGTGATTAAGGCTTTTTTTGAGGCCGAAAAAGCACATAGAGGACAGATGCGTGCAAGTGGCGATCCATACTTCGAGCATTGTGTGGAAACAGCGGTGATGCTTGCGCTTGTTGGTGCTAATTCCACAGTCGTTGCTGCAGGACTCTTGCACGACACACTTGATGATTCTTTTGTGAGTCATGACTACATATTGGGGACGTTCGGAGCTGGGGTTGCTGATTTAGTTGAAGGGGTGTCTAAGCTAAGTCATTTAAGCAAGCTTGCTCGTGAACATGATACGGCTGATAGAATGGTTGAGGCAGACCGCTTGCACACCATGTTCCTCGCTATGGCTGATGCAAGAGCGGTTCTCATTAAATTAGCAGACCGTTTGCACAATATGATGACTTTGGATGCATTGCCTCTGATCAAGCGGCAAAGGTTTGCCAAGGAGACTATGGAGATATTTGTTCCTTTGGCGAATCGCTTAGGAATCTACAGTTGGAAGGAGCAGCTAGAAAACTTGTGTTTTAAGCATCTTAACCTGGAACAGCACAAAGATTTGTCATCCAAACTTATGGGTTTATATGATGAAGCAATTATATATTCTGCAATTCAGAAATTAGAGCGAGCTCTCGAGGATAAAGGAATCTCTTATCATGTTGTAACTGGGCGGCACAAAAGTGTCTACAGCGTTCACTGCAAAATGTTGAAGAAGAATTTGACAATGAATGAGATCCATGATATTCATGGATTGAGGCTCATTGTTGAAAAGGAAGAGGATTGCTATGAAGCTTTGAGAATCGTTCATCAGTTATGGCCCGAAGTACCGGGAAAGCTCAAGGACTACATAAGTAAACCGAAGTTAAATGGGTATTTCTCACACTGCAGTTTCTATTGCTTTATTTCCTATATATTTCTTCTCTTATATAATCTGATGGTAAACAGGTATCAATCTATACACACAGTAGTAAGGGGTGAAGGTGACATCCCACTCGAGGTTCAAATTCGGACCAAGAAGATGCATTTGCAAGCCGAGTTCGGGTTTGCTGCACATTGGAGATACAAAGAGGGTGATTCTAAGCACTCTTCATTTGTGCTTCAGATGGTTGAATGGGCCAGATGGGTTCTCACATGGCATTGTGAGACTATGAGCAAAGATCGATCTTCGATCACCTACGCCGATTCTGTCAAACCACCCTGCAAGTTCCCTTTTCACTCTTCTGACTGCTCGTACTCTTACAAACCTCACTGCGTCCAGGATGGGCCTTTGTTTGTCATTATGATTGAAAATGAAAAGATGTCGGTCCAGGAATTCCCGGCTAATGCAACGATGATGGATCTTTTAGAAAGAGCGGGGAGAGGAAGCACGAGATGGACTCACTACAGGTTCCCAATGAAGGAAGAGTTGAGACCAAGGCTGAACCACAAGCCCGTGAGCGACCCCAAGTGCAAGCTGAAGATGGGCGATGTGGTGGAATTGACCCCGCCGATACCAGACAAGTCGTTGATCGAGTACCGGGAAGAAATCCAGCGAATGTATGAGGGAGGATTTACTGTTGCAACTCCAGAACCTGCAGGTTGGAGTAGC

Protein sequence

MAVPTIALYTSPPSTICSSPHPCHMNSHASYDLEFTSRSSSLPSSTASSSQKPMIGGLSSLFSSTTARHTSSSASISSGGDELGSFRHDKGEELKELSSSFRYSPSKFIGSFFNRDQSPVSVFQGPVSCGSCGVGSAVRTPPLWTGRERSGDASFHGRGSSNRLFNGFVRNALGSCVDYDSPRLEVSSDCLDVGSSALIVDELTFNMEDNIAEGNSESYAKDLLLSAQSKHKIFCDEFVIKAFFEAEKAHRGQMRASGDPYFEHCVETAVMLALVGANSTVVAAGLLHDTLDDSFVSHDYILGTFGAGVADLVEGVSKLSHLSKLAREHDTADRMVEADRLHTMFLAMADARAVLIKLADRLHNMMTLDALPLIKRQRFAKETMEIFVPLANRLGIYSWKEQLENLCFKHLNLEQHKDLSSKLMGLYDEAIIYSAIQKLERALEDKGISYHVVTGRHKSVYSVHCKMLKKNLTMNEIHDIHGLRLIVEKEEDCYEALRIVHQLWPEVPGKLKDYISKPKLNGYFSHCSFYCFISYIFLLLYNLMVNRYQSIHTVVRGEGDIPLEVQIRTKKMHLQAEFGFAAHWRYKEGDSKHSSFVLQMVEWARWVLTWHCETMSKDRSSITYADSVKPPCKFPFHSSDCSYSYKPHCVQDGPLFVIMIENEKMSVQEFPANATMMDLLERAGRGSTRWTHYRFPMKEELRPRLNHKPVSDPKCKLKMGDVVELTPPIPDKSLIEYREEIQRMYEGGFTVATPEPAGWSS
Homology
BLAST of MS016191 vs. NCBI nr
Match: XP_022152608.1 (probable GTP diphosphokinase RSH2, chloroplastic [Momordica charantia])

HSP 1 Score: 1459.1 bits (3776), Expect = 0.0e+00
Identity = 733/761 (96.32%), Postives = 733/761 (96.32%), Query Frame = 0

Query: 1   MAVPTIALYTSPPSTICSSPHPCHMNSHASYDLEFTSRSSSLPSSTASSSQKPMIGGLSS 60
           MAVPTIALYTSPPSTICSSPHPCHMNSHASYDLEFTSRSSSLPSSTASSSQKPMIGGLSS
Sbjct: 1   MAVPTIALYTSPPSTICSSPHPCHMNSHASYDLEFTSRSSSLPSSTASSSQKPMIGGLSS 60

Query: 61  LFSSTTARHTSSSASISSGGDELGSFRHDKGEELKELSSSFRYSPSKFIGSFFNRDQSPV 120
           LFSSTTARHTSSSASISSGGDELGSFRHDKGEELKELSSSFRYSPSKFIGSFFNRDQSPV
Sbjct: 61  LFSSTTARHTSSSASISSGGDELGSFRHDKGEELKELSSSFRYSPSKFIGSFFNRDQSPV 120

Query: 121 SVFQGPVSCGSCGVGSAVRTPPLWTGRERSGDASFHGRGSSNRLFNGFVRNALGSCVDYD 180
           SVFQGPVSCGSCGVGSAVRTPPLWTGRERSGDASFHGRGSSNRLFNGFVRNALGSCVDYD
Sbjct: 121 SVFQGPVSCGSCGVGSAVRTPPLWTGRERSGDASFHGRGSSNRLFNGFVRNALGSCVDYD 180

Query: 181 SPRLEVSSDCLDVGSSALIVDELTFNMEDNIAEGNSESYAKDLLLSAQSKHKIFCDEFVI 240
           SPRLEVSSDCLDVGSSALIVDELTFNMEDNIAEGNSESYAKDLLLSAQSKHKIFCDEFVI
Sbjct: 181 SPRLEVSSDCLDVGSSALIVDELTFNMEDNIAEGNSESYAKDLLLSAQSKHKIFCDEFVI 240

Query: 241 KAFFEAEKAHRGQMRASGDPYFEHCVETAVMLALVGANSTVVAAGLLHDTLDDSFVSHDY 300
           KAFFEAEKAHRGQMRASGDPYFEHCVETAVMLALVGANSTVVAAGLLHDTLDDSFVSHDY
Sbjct: 241 KAFFEAEKAHRGQMRASGDPYFEHCVETAVMLALVGANSTVVAAGLLHDTLDDSFVSHDY 300

Query: 301 ILGTFGAGVADLVEGVSKLSHLSKLAREHDTADRMVEADRLHTMFLAMADARAVLIKLAD 360
           ILGTFGAGVADLVEGVSKLSHLSKLAREHDTADRMVEADRLHTMFLAMADARAVLIKLAD
Sbjct: 301 ILGTFGAGVADLVEGVSKLSHLSKLAREHDTADRMVEADRLHTMFLAMADARAVLIKLAD 360

Query: 361 RLHNMMTLDALPLIKRQRFAKETMEIFVPLANRLGIYSWKEQLENLCFKHLNLEQHKDLS 420
            LHNMMTLDALPLIKRQRFAKETMEIFVPLANRLGIYSWKEQLENLCFKHLNLEQHKDLS
Sbjct: 361 XLHNMMTLDALPLIKRQRFAKETMEIFVPLANRLGIYSWKEQLENLCFKHLNLEQHKDLS 420

Query: 421 SKLMGLYDEAIIYSAIQKLERALEDKGISYHVVTGRHKSVYSVHCKMLKKNLTMNEIHDI 480
           SKLMGLYDEAIIYSAIQKLERALEDKGISYHVVTGRHKSVYSVHCKMLKKNLTMNEIHDI
Sbjct: 421 SKLMGLYDEAIIYSAIQKLERALEDKGISYHVVTGRHKSVYSVHCKMLKKNLTMNEIHDI 480

Query: 481 HGLRLIVEKEEDCYEALRIVHQLWPEVPGKLKDYISKPKLNGYFSHCSFYCFISYIFLLL 540
           HGLRLIVEKEEDCYEALRIVHQLWPEV GKLKDYISKPKLNG                  
Sbjct: 481 HGLRLIVEKEEDCYEALRIVHQLWPEVLGKLKDYISKPKLNG------------------ 540

Query: 541 YNLMVNRYQSIHTVVRGEGDIPLEVQIRTKKMHLQAEFGFAAHWRYKEGDSKHSSFVLQM 600
                  YQSIHTVVRGEGDIPLEVQIRTKKMHLQAEFGFAAHWRYKEGDSKHSSFVLQM
Sbjct: 541 -------YQSIHTVVRGEGDIPLEVQIRTKKMHLQAEFGFAAHWRYKEGDSKHSSFVLQM 600

Query: 601 VEWARWVLTWHCETMSKDRSSITYADSVKPPCKFPFHSSDCSYSYKPHCVQDGPLFVIMI 660
           VEWARWVLTWHCETMSKDRSSITYADSVKPPCK PFHSSDCSYSYKPHCVQDGPLFVIMI
Sbjct: 601 VEWARWVLTWHCETMSKDRSSITYADSVKPPCKLPFHSSDCSYSYKPHCVQDGPLFVIMI 660

Query: 661 ENEKMSVQEFPANATMMDLLERAGRGSTRWTHYRFPMKEELRPRLNHKPVSDPKCKLKMG 720
           ENEKMSVQEFPANATMMDLLERAGRGSTRWTHYRFPMKEELRPRLNHKPVSDPKCKLKMG
Sbjct: 661 ENEKMSVQEFPANATMMDLLERAGRGSTRWTHYRFPMKEELRPRLNHKPVSDPKCKLKMG 720

Query: 721 DVVELTPPIPDKSLIEYREEIQRMYEGGFTVATPEPAGWSS 762
           DVVELTPPIPDKSLIEYREEIQRMYEGGFTVATPEPAGWSS
Sbjct: 721 DVVELTPPIPDKSLIEYREEIQRMYEGGFTVATPEPAGWSS 736

BLAST of MS016191 vs. NCBI nr
Match: XP_038879703.1 (probable GTP diphosphokinase RSH2, chloroplastic [Benincasa hispida])

HSP 1 Score: 1345.9 bits (3482), Expect = 0.0e+00
Identity = 675/761 (88.70%), Postives = 706/761 (92.77%), Query Frame = 0

Query: 1   MAVPTIALYTSPPSTICSSPHPCHMNSHASYDLEFTSRSSSLPSSTASSSQKPMIGGLSS 60
           MAVPTIALYTSPPSTICSSPHPC MNSH+S DLEFTSRSSSL SSTA+SS+KPM+GGLSS
Sbjct: 1   MAVPTIALYTSPPSTICSSPHPCQMNSHSSCDLEFTSRSSSLASSTATSSKKPMVGGLSS 60

Query: 61  LFSSTTARHTSSSASISSGGDELGSFRHDKGEELKELSSSFRYSPSKFIGSFFNRDQSPV 120
           LFSS+  RH+SSS+SISS GDELGSFRHDKG+ELKELSSSF YSP+KFIGSFFNRDQSP+
Sbjct: 61  LFSSSVPRHSSSSSSISSSGDELGSFRHDKGDELKELSSSFHYSPNKFIGSFFNRDQSPI 120

Query: 121 SVFQGPVSCGSCGVGSAVRTPPLWTGRERSGDASFHGRGSSNRLFNGFVRNALGSCVDYD 180
           SVFQGPVSCGS GVGSA RTPPLWT RERSGD SFHGRGS+NRLFNGFVRNALGSCVDYD
Sbjct: 121 SVFQGPVSCGSSGVGSA-RTPPLWTVRERSGDGSFHGRGSTNRLFNGFVRNALGSCVDYD 180

Query: 181 SPRLEVSSDCLDVGSSALIVDELTFNMEDNIAEGNSESYAKDLLLSAQSKHKIFCDEFVI 240
           SPRLEVSSD LDVGSSAL  DELTFNMEDN+ EGNSESYAKDLLLSAQSKHKIFCD+FVI
Sbjct: 181 SPRLEVSSDGLDVGSSALFGDELTFNMEDNVMEGNSESYAKDLLLSAQSKHKIFCDKFVI 240

Query: 241 KAFFEAEKAHRGQMRASGDPYFEHCVETAVMLALVGANSTVVAAGLLHDTLDDSFVSHDY 300
           KAFFEAEKAHRGQMRASGDPY EHCVETAVMLALVGANSTV+AAGLLHDT+DDSFVSHDY
Sbjct: 241 KAFFEAEKAHRGQMRASGDPYLEHCVETAVMLALVGANSTVIAAGLLHDTIDDSFVSHDY 300

Query: 301 ILGTFGAGVADLVEGVSKLSHLSKLAREHDTADRMVEADRLHTMFLAMADARAVLIKLAD 360
           ILG+FGA VADLVEGVSKLSHLSKLAREHDTA+R+VEADRLHTMFLAMADARAVLIKLAD
Sbjct: 301 ILGSFGAEVADLVEGVSKLSHLSKLAREHDTAERIVEADRLHTMFLAMADARAVLIKLAD 360

Query: 361 RLHNMMTLDALPLIKRQRFAKETMEIFVPLANRLGIYSWKEQLENLCFKHLNLEQHKDLS 420
           RLHNMMTLDALPLIKRQRFAKET+EIFVPLANRLGIYSWKEQLENLCFKHLNLEQHKDLS
Sbjct: 361 RLHNMMTLDALPLIKRQRFAKETLEIFVPLANRLGIYSWKEQLENLCFKHLNLEQHKDLS 420

Query: 421 SKLMGLYDEAIIYSAIQKLERALEDKGISYHVVTGRHKSVYSVHCKMLKKNLTMNEIHDI 480
           SKL+GLYDEAII+SA QKLERAL+DKGISYHVVTGRHKSVYS+H KMLKKNLT+NEIHDI
Sbjct: 421 SKLLGLYDEAIIFSATQKLERALKDKGISYHVVTGRHKSVYSIHRKMLKKNLTVNEIHDI 480

Query: 481 HGLRLIVEKEEDCYEALRIVHQLWPEVPGKLKDYISKPKLNGYFSHCSFYCFISYIFLLL 540
           HGLRLIVE E DCYEALRIVHQLWP+VPGK KDYISKPKLNG                  
Sbjct: 481 HGLRLIVENEGDCYEALRIVHQLWPKVPGKFKDYISKPKLNG------------------ 540

Query: 541 YNLMVNRYQSIHTVVRGEGDIPLEVQIRTKKMHLQAEFGFAAHWRYKEGDSKHSSFVLQM 600
                  YQSIHTVV+GEGD+PLEVQIRTK+MHLQAEFGFAAHWRYKEGDSKHSSFVLQM
Sbjct: 541 -------YQSIHTVVKGEGDVPLEVQIRTKEMHLQAEFGFAAHWRYKEGDSKHSSFVLQM 600

Query: 601 VEWARWVLTWHCETMSKDRSSITYADSVKPPCKFPFHSSDCSYSYKPHCVQDGPLFVIMI 660
           VEWARWVLTWHCETM+KDRSSI    SVKPPCKFPFHSSDCSYSYKPH  QDGPLFVIMI
Sbjct: 601 VEWARWVLTWHCETMNKDRSSI---GSVKPPCKFPFHSSDCSYSYKPHYFQDGPLFVIMI 660

Query: 661 ENEKMSVQEFPANATMMDLLERAGRGSTRWTHYRFPMKEELRPRLNHKPVSDPKCKLKMG 720
           ENEKMSVQEFPANATMMDLLERAGRGSTRW HYRFPMKEELRPRLNH+PVSDPKCKLKMG
Sbjct: 661 ENEKMSVQEFPANATMMDLLERAGRGSTRWAHYRFPMKEELRPRLNHEPVSDPKCKLKMG 720

Query: 721 DVVELTPPIPDKSLIEYREEIQRMYEGGFTVATPEPAGWSS 762
           DVVELTPPIPDKSLIEYREEIQRMYEGGFTVATP+PAGW S
Sbjct: 721 DVVELTPPIPDKSLIEYREEIQRMYEGGFTVATPQPAGWRS 732

BLAST of MS016191 vs. NCBI nr
Match: XP_022955553.1 (probable GTP diphosphokinase RSH3, chloroplastic [Cucurbita moschata] >XP_022955554.1 probable GTP diphosphokinase RSH3, chloroplastic [Cucurbita moschata] >XP_022955555.1 probable GTP diphosphokinase RSH3, chloroplastic [Cucurbita moschata])

HSP 1 Score: 1340.5 bits (3468), Expect = 0.0e+00
Identity = 671/761 (88.17%), Postives = 701/761 (92.12%), Query Frame = 0

Query: 1   MAVPTIALYTSPPSTICSSPHPCHMNSHASYDLEFTSRSSSLPSSTASSSQKPMIGGLSS 60
           MAVPTIALYTSPPSTICSSPHPC MN+HAS DL+FTSRSSSL SSTA+SSQKPM+GGLSS
Sbjct: 1   MAVPTIALYTSPPSTICSSPHPCQMNAHASCDLDFTSRSSSLASSTATSSQKPMVGGLSS 60

Query: 61  LFSSTTARHTSSSASISSGGDELGSFRHDKGEELKELSSSFRYSPSKFIGSFFNRDQSPV 120
           LFSST  RH+SSS+SISSGG+ELGSF+HDKGEELKELSSSFRYSP+KFIGSFFNRDQSPV
Sbjct: 61  LFSSTALRHSSSSSSISSGGEELGSFKHDKGEELKELSSSFRYSPNKFIGSFFNRDQSPV 120

Query: 121 SVFQGPVSCGSCGVGSAVRTPPLWTGRERSGDASFHGRGSSNRLFNGFVRNALGSCVDYD 180
           SVFQGPVSCGSCGVGSA RTPPLWT RERSGD SFHGRGS+NRLFNGFVRNALGSCVDYD
Sbjct: 121 SVFQGPVSCGSCGVGSAARTPPLWTVRERSGDGSFHGRGSTNRLFNGFVRNALGSCVDYD 180

Query: 181 SPRLEVSSDCLDVGSSALIVDELTFNMEDNIAEGNSESYAKDLLLSAQSKHKIFCDEFVI 240
           SPRLEVSSD LD  SSAL  DELTFNMED+I EGNS+SY KDLL SAQSKHKIFCDEFVI
Sbjct: 181 SPRLEVSSDRLDASSSALFGDELTFNMEDSITEGNSKSYVKDLLQSAQSKHKIFCDEFVI 240

Query: 241 KAFFEAEKAHRGQMRASGDPYFEHCVETAVMLALVGANSTVVAAGLLHDTLDDSFVSHDY 300
           KAF EAEKAHRGQMRASGDPY EHCVETAVMLALVGANSTVVAAGLLHDTLDDSF+SHDY
Sbjct: 241 KAFNEAEKAHRGQMRASGDPYLEHCVETAVMLALVGANSTVVAAGLLHDTLDDSFLSHDY 300

Query: 301 ILGTFGAGVADLVEGVSKLSHLSKLAREHDTADRMVEADRLHTMFLAMADARAVLIKLAD 360
           IL TFG  VADLVEGVSKLSHLSKLAREHDTA+R+VEADRLHTMFLAMADARAVLIKLAD
Sbjct: 301 ILETFGVEVADLVEGVSKLSHLSKLAREHDTAERIVEADRLHTMFLAMADARAVLIKLAD 360

Query: 361 RLHNMMTLDALPLIKRQRFAKETMEIFVPLANRLGIYSWKEQLENLCFKHLNLEQHKDLS 420
           RLHNMMTLDALPLIKRQRFAKETMEIFVPLANRLGIYSWKEQLENLCFKHLNLE H+DLS
Sbjct: 361 RLHNMMTLDALPLIKRQRFAKETMEIFVPLANRLGIYSWKEQLENLCFKHLNLELHEDLS 420

Query: 421 SKLMGLYDEAIIYSAIQKLERALEDKGISYHVVTGRHKSVYSVHCKMLKKNLTMNEIHDI 480
           SKL+GLYDEAII+SAIQKLERAL+D GISYHVVTGRHKSVYS+H KMLKKNLT+NEIHDI
Sbjct: 421 SKLLGLYDEAIIFSAIQKLERALKDNGISYHVVTGRHKSVYSIHRKMLKKNLTVNEIHDI 480

Query: 481 HGLRLIVEKEEDCYEALRIVHQLWPEVPGKLKDYISKPKLNGYFSHCSFYCFISYIFLLL 540
           HGLRLIVE EEDC+EALRIV QLWPEVPGKLKDYISKPKLNG                  
Sbjct: 481 HGLRLIVENEEDCHEALRIVRQLWPEVPGKLKDYISKPKLNG------------------ 540

Query: 541 YNLMVNRYQSIHTVVRGEGDIPLEVQIRTKKMHLQAEFGFAAHWRYKEGDSKHSSFVLQM 600
                  YQSIHTVVRGEGD+PLEVQIRTK+MHLQAEFGFAAHWRYKEGDSKHSSFVLQM
Sbjct: 541 -------YQSIHTVVRGEGDVPLEVQIRTKEMHLQAEFGFAAHWRYKEGDSKHSSFVLQM 600

Query: 601 VEWARWVLTWHCETMSKDRSSITYADSVKPPCKFPFHSSDCSYSYKPHCVQDGPLFVIMI 660
           VEWARWVLTWHCETM++DRSSI    S+KPPC FPFHSSDCSYSYKPHC QDGPLFVIMI
Sbjct: 601 VEWARWVLTWHCETMNRDRSSI---GSIKPPCNFPFHSSDCSYSYKPHCFQDGPLFVIMI 660

Query: 661 ENEKMSVQEFPANATMMDLLERAGRGSTRWTHYRFPMKEELRPRLNHKPVSDPKCKLKMG 720
           ENEKMSVQEFPAN TMMDLLERAGRGSTRW HYRFP+KEELRPRLNH+PVSDPKCKLKMG
Sbjct: 661 ENEKMSVQEFPANTTMMDLLERAGRGSTRWAHYRFPIKEELRPRLNHEPVSDPKCKLKMG 720

Query: 721 DVVELTPPIPDKSLIEYREEIQRMYEGGFTVATPEPAGWSS 762
           DVVELTPPIPDKSLIEYREEIQRMYEGGFTVATP+PAGW S
Sbjct: 721 DVVELTPPIPDKSLIEYREEIQRMYEGGFTVATPQPAGWRS 733

BLAST of MS016191 vs. NCBI nr
Match: XP_004148566.1 (probable GTP diphosphokinase RSH2, chloroplastic [Cucumis sativus] >XP_031745354.1 probable GTP diphosphokinase RSH2, chloroplastic [Cucumis sativus] >KAE8637300.1 hypothetical protein CSA_004515 [Cucumis sativus] >KGN58500.1 hypothetical protein Csa_002228 [Cucumis sativus])

HSP 1 Score: 1338.9 bits (3464), Expect = 0.0e+00
Identity = 669/761 (87.91%), Postives = 701/761 (92.12%), Query Frame = 0

Query: 1   MAVPTIALYTSPPSTICSSPHPCHMNSHASYDLEFTSRSSSLPSSTASSSQKPMIGGLSS 60
           MAVPTIA YTSPPSTICSSPHPC +N+HAS DLEFTSRSSSL SSTA+SSQKPM+GGLSS
Sbjct: 1   MAVPTIAFYTSPPSTICSSPHPCQINTHASCDLEFTSRSSSLASSTAASSQKPMVGGLSS 60

Query: 61  LFSSTTARHTSSSASISSGGDELGSFRHDKGEELKELSSSFRYSPSKFIGSFFNRDQSPV 120
           LFSST  R +SSSASISSGGDELGSFRHDKG+ELKELSSSFRYSP+KFIGSFFNRDQSPV
Sbjct: 61  LFSSTAPRLSSSSASISSGGDELGSFRHDKGDELKELSSSFRYSPNKFIGSFFNRDQSPV 120

Query: 121 SVFQGPVSCGSCGVGSAVRTPPLWTGRERSGDASFHGRGSSNRLFNGFVRNALGSCVDYD 180
           SVFQGPVSCGSCG GSA RTPPLWT RERSGD SFHGRG +NRLF+GFVRNALGSCVDYD
Sbjct: 121 SVFQGPVSCGSCGFGSAARTPPLWTVRERSGDGSFHGRGGTNRLFSGFVRNALGSCVDYD 180

Query: 181 SPRLEVSSDCLDVGSSALIVDELTFNMEDNIAEGNSESYAKDLLLSAQSKHKIFCDEFVI 240
           SPRLEVSSD LDVGSSAL  DELTFNMEDNI EGNSESYAKDLLLSAQSKHKIFCDEFV+
Sbjct: 181 SPRLEVSSDGLDVGSSALFGDELTFNMEDNITEGNSESYAKDLLLSAQSKHKIFCDEFVV 240

Query: 241 KAFFEAEKAHRGQMRASGDPYFEHCVETAVMLALVGANSTVVAAGLLHDTLDDSFVSHDY 300
           KAFFEAEKAHRGQ+RASGDPY EHCVETAVMLALVGANSTVVAAGLLHDT+DDSFV+HDY
Sbjct: 241 KAFFEAEKAHRGQLRASGDPYLEHCVETAVMLALVGANSTVVAAGLLHDTIDDSFVTHDY 300

Query: 301 ILGTFGAGVADLVEGVSKLSHLSKLAREHDTADRMVEADRLHTMFLAMADARAVLIKLAD 360
           ILGTFGA VADLVEGVSKLSHLSKLAREHDTA+R VEADRLHTMFLAMADARAVL+KLAD
Sbjct: 301 ILGTFGAEVADLVEGVSKLSHLSKLAREHDTAERTVEADRLHTMFLAMADARAVLVKLAD 360

Query: 361 RLHNMMTLDALPLIKRQRFAKETMEIFVPLANRLGIYSWKEQLENLCFKHLNLEQHKDLS 420
           RLHNMMTLDALP IK+QRFAKETMEIFVPLANRLGIY+WKEQLEN+CFKHLNLEQH+DLS
Sbjct: 361 RLHNMMTLDALPPIKQQRFAKETMEIFVPLANRLGIYTWKEQLENMCFKHLNLEQHEDLS 420

Query: 421 SKLMGLYDEAIIYSAIQKLERALEDKGISYHVVTGRHKSVYSVHCKMLKKNLTMNEIHDI 480
           SKL+GLYDEAII+SA QKLERAL+DKGISYHVVTGRHKSVYS+H KMLKKNLT+NEIHDI
Sbjct: 421 SKLLGLYDEAIIFSATQKLERALKDKGISYHVVTGRHKSVYSIHRKMLKKNLTVNEIHDI 480

Query: 481 HGLRLIVEKEEDCYEALRIVHQLWPEVPGKLKDYISKPKLNGYFSHCSFYCFISYIFLLL 540
           HGLRLIVE EEDCYEALRIVHQLWP VPGKLKDYISKPKLNG                  
Sbjct: 481 HGLRLIVENEEDCYEALRIVHQLWPNVPGKLKDYISKPKLNG------------------ 540

Query: 541 YNLMVNRYQSIHTVVRGEGDIPLEVQIRTKKMHLQAEFGFAAHWRYKEGDSKHSSFVLQM 600
                  YQSIHTVVRGEGD+PLEVQIRTK+MHLQAEFGFAAHWRYKEGDSKHSSFVLQM
Sbjct: 541 -------YQSIHTVVRGEGDVPLEVQIRTKEMHLQAEFGFAAHWRYKEGDSKHSSFVLQM 600

Query: 601 VEWARWVLTWHCETMSKDRSSITYADSVKPPCKFPFHSSDCSYSYKPHCVQDGPLFVIMI 660
           VEWARWVLTWHCETM+KDR SI    SV+PPCKFPFHSSDCSYSYKP   QDGPLFVIMI
Sbjct: 601 VEWARWVLTWHCETMNKDRPSI---GSVRPPCKFPFHSSDCSYSYKPRYFQDGPLFVIMI 660

Query: 661 ENEKMSVQEFPANATMMDLLERAGRGSTRWTHYRFPMKEELRPRLNHKPVSDPKCKLKMG 720
           ENEKMSVQEFPA+ATMMDLLERAGRGSTRW HYRFPMKEELRPRLNH+PVSDPKCKLKMG
Sbjct: 661 ENEKMSVQEFPADATMMDLLERAGRGSTRWAHYRFPMKEELRPRLNHEPVSDPKCKLKMG 720

Query: 721 DVVELTPPIPDKSLIEYREEIQRMYEGGFTVATPEPAGWSS 762
           DVVELTPPIPDK L+EYREEIQRMYEGGFTVATP+PAGW S
Sbjct: 721 DVVELTPPIPDKLLVEYREEIQRMYEGGFTVATPQPAGWKS 733

BLAST of MS016191 vs. NCBI nr
Match: KAG7018260.1 (putative GTP diphosphokinase RSH3, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1338.2 bits (3462), Expect = 0.0e+00
Identity = 669/761 (87.91%), Postives = 701/761 (92.12%), Query Frame = 0

Query: 1   MAVPTIALYTSPPSTICSSPHPCHMNSHASYDLEFTSRSSSLPSSTASSSQKPMIGGLSS 60
           MAVPTIALYTSPPSTICSSPHPC MN+HAS DL+FTSRSSSL SSTA+SSQKPM+GGLSS
Sbjct: 1   MAVPTIALYTSPPSTICSSPHPCQMNAHASCDLDFTSRSSSLASSTATSSQKPMVGGLSS 60

Query: 61  LFSSTTARHTSSSASISSGGDELGSFRHDKGEELKELSSSFRYSPSKFIGSFFNRDQSPV 120
           LFSST  RH+SSS+SI+SGG+ELGSF+HDKGEELKELSSSFRYSP+KFIGSFFNRDQSPV
Sbjct: 61  LFSSTALRHSSSSSSITSGGEELGSFKHDKGEELKELSSSFRYSPNKFIGSFFNRDQSPV 120

Query: 121 SVFQGPVSCGSCGVGSAVRTPPLWTGRERSGDASFHGRGSSNRLFNGFVRNALGSCVDYD 180
           SVFQGPVSCGSCGVGSA RTPPLWT RERSGD SFHGRGS+NRLFNGFVRNALGSCVDYD
Sbjct: 121 SVFQGPVSCGSCGVGSAARTPPLWTVRERSGDGSFHGRGSTNRLFNGFVRNALGSCVDYD 180

Query: 181 SPRLEVSSDCLDVGSSALIVDELTFNMEDNIAEGNSESYAKDLLLSAQSKHKIFCDEFVI 240
           SPRLEVSSD LD  SSAL  DELTFNMED+I EGNS+SY KDLL SAQSKHKIFCDEFVI
Sbjct: 181 SPRLEVSSDRLDASSSALFGDELTFNMEDSITEGNSKSYVKDLLQSAQSKHKIFCDEFVI 240

Query: 241 KAFFEAEKAHRGQMRASGDPYFEHCVETAVMLALVGANSTVVAAGLLHDTLDDSFVSHDY 300
           KAF EAEKAHRGQMRASGDPY EHCVETAVMLALVGANSTVVAAGLLHDTLDDSF+SHDY
Sbjct: 241 KAFNEAEKAHRGQMRASGDPYLEHCVETAVMLALVGANSTVVAAGLLHDTLDDSFLSHDY 300

Query: 301 ILGTFGAGVADLVEGVSKLSHLSKLAREHDTADRMVEADRLHTMFLAMADARAVLIKLAD 360
           IL TFG  VADLVEGVSKLSHLSKLAREHDTA+R+VEADRLHTMFLAMADARAVLIKLAD
Sbjct: 301 ILETFGVEVADLVEGVSKLSHLSKLAREHDTAERIVEADRLHTMFLAMADARAVLIKLAD 360

Query: 361 RLHNMMTLDALPLIKRQRFAKETMEIFVPLANRLGIYSWKEQLENLCFKHLNLEQHKDLS 420
           RLHNMMTLDALPL+KRQRFAKETMEIFVPLANRLGIYSWKEQLENLCFKHLNLE H+DLS
Sbjct: 361 RLHNMMTLDALPLMKRQRFAKETMEIFVPLANRLGIYSWKEQLENLCFKHLNLELHEDLS 420

Query: 421 SKLMGLYDEAIIYSAIQKLERALEDKGISYHVVTGRHKSVYSVHCKMLKKNLTMNEIHDI 480
           SKL+GLYDEAII+SAIQKLERAL+D GISYHVVTGRHKSVYS+H KMLKKNLT+NEIHDI
Sbjct: 421 SKLLGLYDEAIIFSAIQKLERALKDSGISYHVVTGRHKSVYSIHRKMLKKNLTVNEIHDI 480

Query: 481 HGLRLIVEKEEDCYEALRIVHQLWPEVPGKLKDYISKPKLNGYFSHCSFYCFISYIFLLL 540
           HGLRLIVE EEDC+EALRIV QLWPEVPGKLKDYISKPKLNG                  
Sbjct: 481 HGLRLIVENEEDCHEALRIVRQLWPEVPGKLKDYISKPKLNG------------------ 540

Query: 541 YNLMVNRYQSIHTVVRGEGDIPLEVQIRTKKMHLQAEFGFAAHWRYKEGDSKHSSFVLQM 600
                  YQSIHTVVRGEGD+PLEVQIRTK+MHLQAEFGFAAHWRYKEGDSKHSSFVLQM
Sbjct: 541 -------YQSIHTVVRGEGDVPLEVQIRTKEMHLQAEFGFAAHWRYKEGDSKHSSFVLQM 600

Query: 601 VEWARWVLTWHCETMSKDRSSITYADSVKPPCKFPFHSSDCSYSYKPHCVQDGPLFVIMI 660
           VEWARWVLTWHCETM++DRSSI    S+KPPC FPFHSSDCSYSYKPHC QDGPLFVIMI
Sbjct: 601 VEWARWVLTWHCETMNRDRSSI---GSIKPPCNFPFHSSDCSYSYKPHCFQDGPLFVIMI 660

Query: 661 ENEKMSVQEFPANATMMDLLERAGRGSTRWTHYRFPMKEELRPRLNHKPVSDPKCKLKMG 720
           ENEKMSVQEFPAN TMMDLLERAGRGSTRW HYRFP+KEELRPRLNH+PVSDPKCKLKMG
Sbjct: 661 ENEKMSVQEFPANTTMMDLLERAGRGSTRWAHYRFPIKEELRPRLNHEPVSDPKCKLKMG 720

Query: 721 DVVELTPPIPDKSLIEYREEIQRMYEGGFTVATPEPAGWSS 762
           DVVELTPPIPDKSLIEYREEIQRMYEGGFTVATP+PAGW S
Sbjct: 721 DVVELTPPIPDKSLIEYREEIQRMYEGGFTVATPQPAGWRS 733

BLAST of MS016191 vs. ExPASy Swiss-Prot
Match: Q9LVJ3 (Probable GTP diphosphokinase RSH2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=RSH2 PE=2 SV=1)

HSP 1 Score: 922.5 bits (2383), Expect = 3.0e-267
Identity = 494/762 (64.83%), Postives = 581/762 (76.25%), Query Frame = 0

Query: 5   TIALYTSPPSTICSSPHPCHMNSHASYDLEFTSRSSSLPSSTASSSQKPMIGGLSSLFSS 64
           TIALY SPPS++CS+PH        S DL+ TSRSSS  SS ASS QKP++GGLSSLFSS
Sbjct: 6   TIALYASPPSSVCSTPH------QISCDLDLTSRSSSTSSSMASSPQKPIVGGLSSLFSS 65

Query: 65  TTARHTSSSA-SISSGGDELGSFRHDKGEELKEL--SSSFRYSPSKFIGSFFNRDQSPVS 124
            + + +SSS+ S S+G DE  S R+D+ ++LK+L  SSSF YSP+KF+ SF    QSP+S
Sbjct: 66  ASVKSSSSSSCSYSTGVDEFSSLRYDRSDDLKDLSFSSSFGYSPAKFVNSFRRDHQSPIS 125

Query: 125 VFQGPVSCGSCGVGSAVRTPPLWTGRERSGDASFHGRGSSNRLFNGFVRNALGSCVDYDS 184
           V  GPVSC SC       +PP+   R+R+ D SF  R  ++ LFNGFVR ALGSCVDY  
Sbjct: 126 VLHGPVSC-SC-------SPPMRMSRDRNLDGSF--RLGASGLFNGFVRKALGSCVDY-- 185

Query: 185 PRLEVSSDCLDVGSSALIVDELTFNME-DNIAEGNSESYAKDLLLSAQSKHKIFCDEFVI 244
                     + GS +++VDELTF ME D I     + YA+DLL  AQ +HKIF DE VI
Sbjct: 186 ----------EFGSDSVLVDELTFPMEVDTI-----KPYARDLLRRAQLRHKIFNDESVI 245

Query: 245 KAFFEAEKAHRGQMRASGDPYFEHCVETAVMLALVGANSTVVAAGLLHDTLDDSFVSHDY 304
           KAF+EAEKAHRGQMRAS DPY +HCVETA++LA +GANSTVV AGLLHDT+DDSF+S+DY
Sbjct: 246 KAFYEAEKAHRGQMRASRDPYLQHCVETAMLLANIGANSTVVVAGLLHDTIDDSFMSYDY 305

Query: 305 ILGTFGAGVADLVEGVSKLSHLSKLAREHDTADRMVEADRLHTMFLAMADARAVLIKLAD 364
           IL  FGAGVADLVEGVSKLS LSKLARE++TA + VEADRLHTMFLAMADARAVLIKLAD
Sbjct: 306 ILRNFGAGVADLVEGVSKLSQLSKLARENNTACKTVEADRLHTMFLAMADARAVLIKLAD 365

Query: 365 RLHNMMTLDALPLIKRQRFAKETMEIFVPLANRLGIYSWKEQLENLCFKHLNLEQHKDLS 424
           RLHNM TL AL  +K+QRFAKET+EIF PLANRLGI +WK QLENLCFKHL   QH ++S
Sbjct: 366 RLHNMKTLYALSPVKQQRFAKETLEIFAPLANRLGISTWKVQLENLCFKHLYPNQHNEMS 425

Query: 425 SKLMGLYDEAIIYSAIQKLERALEDKGISYHVVTGRHKSVYSVHCKMLKKNLTMNEIHDI 484
           + L   +DEA+I SAI+KLE+AL+  GISYHV+ GRHKS+YS++ KMLKK LT++EIHDI
Sbjct: 426 TMLEDSFDEAMITSAIEKLEQALKKAGISYHVLCGRHKSLYSIYSKMLKKKLTVDEIHDI 485

Query: 485 HGLRLIVEKEEDCYEALRIVHQLWPEVPGKLKDYISKPKLNGYFSHCSFYCFISYIFLLL 544
           HGLRLIV+ E DCY+AL +VH LW EVPGKLKDYI+ PK NG                  
Sbjct: 486 HGLRLIVDNEGDCYKALGVVHSLWSEVPGKLKDYITHPKFNG------------------ 545

Query: 545 YNLMVNRYQSIHTVVRGEGDIPLEVQIRTKKMHLQAEFGFAAHWRYKEGDSKHSSFVLQM 604
                  YQS+HTVV   G +PLEVQIRT++MHLQAEFGFAAHWRYKEG  K+SSFVLQM
Sbjct: 546 -------YQSLHTVVMDNGTVPLEVQIRTQEMHLQAEFGFAAHWRYKEGGCKYSSFVLQM 605

Query: 605 VEWARWVLTWHCETMSKDRSSITYADSVKPPCKFPFHSSDCSYSYKPHCVQDGPLFVIMI 664
           VEWARWV+TWHCE MSKDRSSI+ +DS+KPPCKFP HS DC  SYKP+  QDGP++VI+I
Sbjct: 606 VEWARWVVTWHCEAMSKDRSSISSSDSIKPPCKFPSHSEDCPASYKPNSSQDGPVYVIVI 665

Query: 665 ENEKMSVQEFPANATMMDLLERAGRGSTRWTHYRFPMKEELRPRLNHKPVSDPKCKLKMG 724
           EN+KMSVQEFPA++T+ DLL RAG GS+RW+ Y  P KEELRPRLN  PVSD K KLKMG
Sbjct: 666 ENDKMSVQEFPASSTVSDLLSRAGPGSSRWSMYGIPAKEELRPRLNQIPVSDLKWKLKMG 709

Query: 725 DVVELTPPIPDKSLIEYREEIQRMYEGGFTVATP-EPAGWSS 762
           DVVELTP IPD+SL EYREEIQRMY+ G   + P    GW S
Sbjct: 726 DVVELTPTIPDESLTEYREEIQRMYDRGLAFSRPGTMVGWGS 709

BLAST of MS016191 vs. ExPASy Swiss-Prot
Match: Q9SYH1 (Probable GTP diphosphokinase RSH3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=RSH3 PE=2 SV=1)

HSP 1 Score: 919.8 bits (2376), Expect = 2.0e-266
Identity = 494/766 (64.49%), Postives = 578/766 (75.46%), Query Frame = 0

Query: 5   TIALYTSPPSTICSSPHPCHMNSHASYDLEFTSRSSSLPSSTASSSQKPMIGGLSSLFSS 64
           TIALY SP ST+CS+ H   +N+H S DL+  SRSSS  SST+S    P IGGLS LFS 
Sbjct: 6   TIALYASPASTVCSTAH--QINAHISCDLDLNSRSSSASSSTSS----PTIGGLSLLFSG 65

Query: 65  TTARHTSSSASI-SSGGDELGSFRHDKGEELKELSSSFRYSPSKFIG-SFFNRD-QSPVS 124
            + + +SSS+S   S G+EL S RHD+ E+ + LS SF YSPSKFIG S+  RD QSPVS
Sbjct: 66  ASVKSSSSSSSSHPSVGEELASIRHDRSED-RTLSGSFCYSPSKFIGSSYLKRDHQSPVS 125

Query: 125 VFQGPVSCGSCGVGSAVRTPPLWTGRERSGDASFHGRGSSNRLFNGFVRNALGSCVDYDS 184
           V  GP+S G+        +PP+   R+R+ D     R  S+RLFNGFVR A+GSCVDYD+
Sbjct: 126 VLHGPISSGN--------SPPMRISRDRNLDGGSALRVGSSRLFNGFVRKAIGSCVDYDT 185

Query: 185 PRLEVSSDCLDVGSSALIVDELTFNMEDNIAEGNSESYAKDLLLSAQSKHKIFCDEFVIK 244
                         S L+ ++L F M+D       + YA+DLL  AQ KHKIF DE VIK
Sbjct: 186 -------------DSVLVDEQLPFTMDDGFEGERRQPYARDLLRRAQLKHKIFEDESVIK 245

Query: 245 AFFEAEKAHRGQMRASGDPYFEHCVETAVMLALVGANSTVVAAGLLHDTLDDSFVSHDYI 304
           AF+EAEKAHRGQMRA+GDPY +HCVETA++LA +GANSTVV AG+LHDTLDDSF+S+DYI
Sbjct: 246 AFYEAEKAHRGQMRATGDPYLQHCVETAMLLADIGANSTVVVAGILHDTLDDSFMSYDYI 305

Query: 305 LGTFGAGVADLVEGVSKLSHLSKLAREHDTADRMVEADRLHTMFLAMADARAVLIKLADR 364
           L TFG+GVADLVEGVSKLS LSKLARE++TA + VEADRLHTMFLAMADARAVLIKLADR
Sbjct: 306 LRTFGSGVADLVEGVSKLSQLSKLARENNTACKTVEADRLHTMFLAMADARAVLIKLADR 365

Query: 365 LHNMMTLDALPLIKRQRFAKETMEIFVPLANRLGIYSWKEQLENLCFKHLNLEQHKDLSS 424
           LHNMMTL ALP +KRQRFAKET+EIF PLANRLGI SWK +LENLCFKHL+ +QH ++S 
Sbjct: 366 LHNMMTLYALPPVKRQRFAKETLEIFAPLANRLGISSWKVKLENLCFKHLHPDQHHEMSD 425

Query: 425 KLMGLYDEAIIYSAIQKLERALEDKGISYHVVTGRHKSVYSVHCKMLKKNLTMNEIHDIH 484
            L   +DEA+I SAI+KLE+AL+ +GISYHVV+GRHKS+YS++CKMLKK LTM+EIHDIH
Sbjct: 426 MLEDSFDEAMITSAIEKLEQALKKEGISYHVVSGRHKSLYSIYCKMLKKKLTMDEIHDIH 485

Query: 485 GLRLIVEKEEDCYEALRIVHQLWPEVPGKLKDYISKPKLNGYFSHCSFYCFISYIFLLLY 544
           GLRLIV+ E+DCY+AL +VH+LW EVPGKLKDYIS PK NG                   
Sbjct: 486 GLRLIVDNEKDCYKALGVVHKLWSEVPGKLKDYISHPKFNG------------------- 545

Query: 545 NLMVNRYQSIHTVVRGEGDIPLEVQIRTKKMHLQAEFGFAAHWRYKEGDSKHSSFVLQMV 604
                 YQS+HTVV G+G IPLEVQIRTK+MHLQAEFGFAAHWRYKEGD KHSSFVLQMV
Sbjct: 546 ------YQSLHTVVMGDGTIPLEVQIRTKEMHLQAEFGFAAHWRYKEGDCKHSSFVLQMV 605

Query: 605 EWARWVLTWHCETMSKDRSSITYADSVKPPCKFPFHSSDCSYSYKPHCVQDGPLFVIMIE 664
           EWARWV+TWH ETMSKD SSI    S +P C FP H+ DC +SYKP   Q+GP++VI+IE
Sbjct: 606 EWARWVVTWHFETMSKDGSSIC---SSEPLCSFPSHAEDCPFSYKPSGNQEGPVYVIVIE 665

Query: 665 NEKMSVQEFPANATMMDLLERAGRGSTRWTHYRFPMKEELRPRLNHKPVSDPKCKLKMGD 724
           NEKM+VQEFP N+T+ DLL RAG GS+RW+ Y  P KEELRPRLN  PVSD KCKLKMGD
Sbjct: 666 NEKMTVQEFPENSTVSDLLRRAGPGSSRWSMYSIPAKEELRPRLNQTPVSDLKCKLKMGD 715

Query: 725 VVELTPPIPDKSLIEYREEIQRMYEGGFTVATPEPA------GWSS 762
           VVELTP IPDKSL EYREEIQRMY+ G   + P  A      GW S
Sbjct: 726 VVELTPAIPDKSLTEYREEIQRMYDRGLAFSRPHRAATGTMVGWGS 715

BLAST of MS016191 vs. ExPASy Swiss-Prot
Match: Q9M5P5 (Probable GTP diphosphokinase RSH3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=RSH3 PE=2 SV=1)

HSP 1 Score: 911.0 bits (2353), Expect = 9.1e-264
Identity = 492/766 (64.23%), Postives = 576/766 (75.20%), Query Frame = 0

Query: 5   TIALYTSPPSTICSSPHPCHMNSHASYDLEFTSRSSSLPSSTASSSQKPMIGGLSSLFSS 64
           TIALY SP ST+CS+ H   +N+H S DL+  SRSSS  SST+S    P IGGLS LFS 
Sbjct: 6   TIALYASPASTVCSTAH--QINAHISCDLDLNSRSSSASSSTSS----PTIGGLSLLFSG 65

Query: 65  TTARHTSSSASI-SSGGDELGSFRHDKGEELKELSSSFRYSPSKFIG-SFFNRD-QSPVS 124
            + + +SSS+S   S G+EL S RHD+ E+ + LS SF YSPSKFIG S+  RD QSPVS
Sbjct: 66  ASVKSSSSSSSSHPSVGEELASIRHDRSED-RTLSGSFCYSPSKFIGSSYLKRDHQSPVS 125

Query: 125 VFQGPVSCGSCGVGSAVRTPPLWTGRERSGDASFHGRGSSNRLFNGFVRNALGSCVDYDS 184
           V  GP+S G+        +PP+   R+R+ D     R  S+RLFNGFVR A+GSCVDYD+
Sbjct: 126 VLHGPISSGN--------SPPMRISRDRNLDGGSALRVGSSRLFNGFVRKAIGSCVDYDT 185

Query: 185 PRLEVSSDCLDVGSSALIVDELTFNMEDNIAEGNSESYAKDLLLSAQSKHKIFCDEFVIK 244
                         S L+ ++L F M+D       + YA+DLL  AQ KHKIF DE VIK
Sbjct: 186 -------------DSVLVDEQLPFTMDDGFEGERRQPYARDLLRRAQLKHKIFEDESVIK 245

Query: 245 AFFEAEKAHRGQMRASGDPYFEHCVETAVMLALVGANSTVVAAGLLHDTLDDSFVSHDYI 304
           AF+EAEKAHRGQMRA+GDPY +HCVETA++LA +GANSTVV AG+LHDTLDDSF+S+DYI
Sbjct: 246 AFYEAEKAHRGQMRATGDPYLQHCVETAMLLADIGANSTVVVAGILHDTLDDSFMSYDYI 305

Query: 305 LGTFGAGVADLVEGVSKLSHLSKLAREHDTADRMVEADRLHTMFLAMADARAVLIKLADR 364
           L TFG+GVADLVEGVS+   LSKLARE++TA + VEADRLHTMFLAMADARAVLIKLADR
Sbjct: 306 LRTFGSGVADLVEGVSQ---LSKLARENNTACKTVEADRLHTMFLAMADARAVLIKLADR 365

Query: 365 LHNMMTLDALPLIKRQRFAKETMEIFVPLANRLGIYSWKEQLENLCFKHLNLEQHKDLSS 424
           LHNMMTL ALP +KRQRFAKET+EIF PLANRLGI SWK +LENLCFKHL+ +QH ++S 
Sbjct: 366 LHNMMTLYALPPVKRQRFAKETLEIFAPLANRLGISSWKVKLENLCFKHLHPDQHHEMSD 425

Query: 425 KLMGLYDEAIIYSAIQKLERALEDKGISYHVVTGRHKSVYSVHCKMLKKNLTMNEIHDIH 484
            L   +DEA+I SAI+KLE+AL+ +GISYHVV+GRHKS+YS++CKMLKK LTM+EIHDIH
Sbjct: 426 MLEDSFDEAMITSAIEKLEQALKKEGISYHVVSGRHKSLYSIYCKMLKKKLTMDEIHDIH 485

Query: 485 GLRLIVEKEEDCYEALRIVHQLWPEVPGKLKDYISKPKLNGYFSHCSFYCFISYIFLLLY 544
           GLRLIV+ E+DCY+AL +VH+LW EVPGKLKDYIS PK NG                   
Sbjct: 486 GLRLIVDNEKDCYKALGVVHKLWSEVPGKLKDYISHPKFNG------------------- 545

Query: 545 NLMVNRYQSIHTVVRGEGDIPLEVQIRTKKMHLQAEFGFAAHWRYKEGDSKHSSFVLQMV 604
                 YQS+HTVV G+G IPLEVQIRTK+MHLQAEFGFAAHWRYKEGD KHSSFVLQMV
Sbjct: 546 ------YQSLHTVVMGDGTIPLEVQIRTKEMHLQAEFGFAAHWRYKEGDCKHSSFVLQMV 605

Query: 605 EWARWVLTWHCETMSKDRSSITYADSVKPPCKFPFHSSDCSYSYKPHCVQDGPLFVIMIE 664
           EWARWV+TWH ETMSKD SSI    S +P C FP H+ DC +SYKP   Q+GP++VI+IE
Sbjct: 606 EWARWVVTWHFETMSKDGSSIC---SSEPLCSFPSHAEDCPFSYKPSGNQEGPVYVIVIE 665

Query: 665 NEKMSVQEFPANATMMDLLERAGRGSTRWTHYRFPMKEELRPRLNHKPVSDPKCKLKMGD 724
           NEKMSVQEFP N+T+ DLL RAG GS+RW+ Y  P KEELRPRLN  PVSD KCKLKMGD
Sbjct: 666 NEKMSVQEFPENSTVSDLLRRAGPGSSRWSMYSIPAKEELRPRLNQTPVSDLKCKLKMGD 712

Query: 725 VVELTPPIPDKSLIEYREEIQRMYEGGFTVATPEPA------GWSS 762
           VVELTP IPDKSL EYREEIQRMY+ G   + P  A      GW S
Sbjct: 726 VVELTPAIPDKSLTEYREEIQRMYDRGLAFSRPHRAATGTMVGWGS 712

BLAST of MS016191 vs. ExPASy Swiss-Prot
Match: Q9M5P6 (Probable GTP diphosphokinase RSH2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=RSH2 PE=2 SV=1)

HSP 1 Score: 896.0 bits (2314), Expect = 3.0e-259
Identity = 486/763 (63.70%), Postives = 575/763 (75.36%), Query Frame = 0

Query: 5   TIALYTSPPSTICSSPHPCHMNSHASYDLEFTSRSSSLPSSTASSSQKPMIGGLSSLFSS 64
           TIALY SPPS++CS+PH        S DL+ TSRSSS  SS ASS QKP++GGLSSLFSS
Sbjct: 6   TIALYASPPSSVCSTPH------QISCDLDLTSRSSSTSSSMASSPQKPIVGGLSSLFSS 65

Query: 65  TTARHTSSSA-SISSGGDELGSFRHDKGEELKEL--SSSFRYSPSKFIGSFFNRDQSPVS 124
            + + +SSS+ S S+  DE  S R+D+ ++LK+L  SSSF YSP+KF+ SF    QSP+S
Sbjct: 66  ASVKSSSSSSCSYSTAVDEFSSLRYDRSDDLKDLSFSSSFGYSPAKFVNSFRRDHQSPIS 125

Query: 125 VFQGPVSCGSCGVGSAVRTPPLWTGRERSGDASFHGRGSSNRLFNGFVRNALGSCVDYDS 184
           V  GPVSC SC       +PP+   R+R+ D SF  R  ++RLFNGFVR ALGSCVDY  
Sbjct: 126 VLHGPVSC-SC-------SPPMRMSRDRNLDGSF--RLGASRLFNGFVRKALGSCVDY-- 185

Query: 185 PRLEVSSDCLDVGSSALIVDELTFNME-DNIAEGNSESYAKDLLLSAQSKHKIFCDEFVI 244
                     ++GS + +VDELTF ME D I     + YA+DLL  AQ +HKIF DE VI
Sbjct: 186 ----------ELGSDSGLVDELTFPMEVDTI-----KPYARDLLRRAQLRHKIFNDESVI 245

Query: 245 KAFFEAEKAHRGQMRASGDPYFEHCVETAVMLALVGANSTVVAAGLLHDTLDDSFVSHDY 304
           KAF+EAEKAHRGQMRAS DPY +HCVETA++LA +GANSTVV AGLLHDT+DDSF+S+DY
Sbjct: 246 KAFYEAEKAHRGQMRASRDPYLQHCVETAMLLANIGANSTVVVAGLLHDTVDDSFMSYDY 305

Query: 305 ILGTFGAGVADLVEGVSKLSHLSKLAREHDTADRMVEADRLHTMFLAMADARAVLIKLAD 364
           IL  FGAGVADLVEGVSKLS LSKLARE++TA + VEADRLH MFLAMADARAVLIKLAD
Sbjct: 306 ILRNFGAGVADLVEGVSKLSQLSKLARENNTACKTVEADRLHPMFLAMADARAVLIKLAD 365

Query: 365 RLHNMMTLDALPLIKRQRFAKETMEIFVPLANRLGIYSWKEQLENLCFKHLNLEQHKDLS 424
           RLHNM TL AL  +K+QRFAKET+EIF PLAN LGI +WK QLENLCFKHL   QH ++S
Sbjct: 366 RLHNMKTLYALSPVKQQRFAKETLEIFAPLANCLGISTWKVQLENLCFKHLYPNQHNEMS 425

Query: 425 SKLMGLYDEAIIYSAIQKLERALEDKGISYHVVTGRHKSVYSVHCKMLKKNLTMNEIHDI 484
           + L   +DEA+I SAI+KL++AL+  GISYHV+ GRHKS+YS++ KMLKK LT++EIHDI
Sbjct: 426 TMLEDSFDEAMITSAIEKLDQALKKAGISYHVLCGRHKSLYSIYSKMLKKKLTVDEIHDI 485

Query: 485 HGLRLIVEKEEDCYEALRIVHQLWPEVPGKLKDYISKPKLNGYFSHCSFYCFISYIFLLL 544
           HGLRLIV+ E DCY+AL +VH LW EVPGKLKDYI+ PK NG                  
Sbjct: 486 HGLRLIVDNEGDCYKALGVVHSLWSEVPGKLKDYITHPKFNG------------------ 545

Query: 545 YNLMVNRYQSIHTVVRGEGDIPLEVQIRTKKMHLQAEFGFAAHWRYKEGDSKHSSFVLQM 604
                  YQS+HTVV   G +PLEVQIRT++MHLQAEFGFAAHWRYKEG  K+SSFVLQM
Sbjct: 546 -------YQSLHTVVMDNGTVPLEVQIRTQEMHLQAEFGFAAHWRYKEGGCKYSSFVLQM 605

Query: 605 VEWARWVLTWHCETMSKDRSSITYADSVKPPCK-FPFHSSDCSYSYKPHCVQDGPLFVIM 664
           VEWARWV+TWHCE MSKDRSSI+ +DS+KPP + F     DC  SYKP+  QDGP++VI+
Sbjct: 606 VEWARWVVTWHCEAMSKDRSSISSSDSIKPPLQVFRLTLEDCPASYKPNSSQDGPVYVIV 665

Query: 665 IENEKMSVQEFPANATMMDLLERAGRGSTRWTHYRFPMKEELRPRLNHKPVSDPKCKLKM 724
           IEN+KMSVQEFPA++T+ DLL RAG GS+RW+ Y  P KEELRPRLN  PVSD K KLKM
Sbjct: 666 IENDKMSVQEFPASSTVSDLLSRAGPGSSRWSMYGIPAKEELRPRLNQIPVSDLKWKLKM 710

Query: 725 GDVVELTPPIPDKSLIEYREEIQRMYEGGFTVATP-EPAGWSS 762
           GDVVELTP IPD+SL EYREEIQRMY+ G   + P    GW S
Sbjct: 726 GDVVELTPKIPDESLTEYREEIQRMYDRGLAFSRPGTMVGWGS 710

BLAST of MS016191 vs. ExPASy Swiss-Prot
Match: Q67UU0 (Probable GTP diphosphokinase RSH3, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=RSH3 PE=2 SV=1)

HSP 1 Score: 825.9 bits (2132), Expect = 3.8e-238
Identity = 454/770 (58.96%), Postives = 551/770 (71.56%), Query Frame = 0

Query: 1   MAVPTIALYTSPPSTICSSPHPCHMNSHASYDLEF--TSRSSSLPSSTA--SSSQKP--M 60
           M++P I+LYTSPP               A Y  EF  +SR SS P STA  S+S +P   
Sbjct: 1   MSLPAISLYTSPP-------------PGAVYSSEFDPSSRGSSPPCSTAPPSTSHRPPAA 60

Query: 61  IGGLSSLFSSTTARHTSSSASISSGGDELGSFRHDKGEELKELSSSFRY--SPSKFIGSF 120
            GGLS LFSS  A   ++S   +   DELG+   D+ +E         Y  SP K+    
Sbjct: 61  AGGLSCLFSSPAA---AASPPRAPPHDELGALWQDRSDEPAFAGGGGGYSSSPLKWRDLH 120

Query: 121 FNRDQSPVSVFQGPVSCGSCGVGSAVRTPPL-W-TGRERSGDASFHGRGSSNRLFNGFVR 180
            +   SPVSVFQGP S       +A R+PP  W  GR+R  +          RLF GFVR
Sbjct: 121 HHHHHSPVSVFQGPSSS-----PAASRSPPASWLAGRDRDRE----------RLFAGFVR 180

Query: 181 NALGSCVDYD---SPRLEVSSDCLDVGSSALIVDELTFNMEDNIAEGN--SESYAKDLLL 240
           NALGSCVDY    SPR EV               EL F +++N+AE +   E  A++LL 
Sbjct: 181 NALGSCVDYAPALSPRSEVGG------------GELAFELDENLAEASPACEPCARELLA 240

Query: 241 SAQSKHKIFCDEFVIKAFFEAEKAHRGQMRASGDPYFEHCVETAVMLALVGANSTVVAAG 300
            AQ++H+IF +E V+K FFEAEKAHRGQ RASGDPY +HCVETAV+LA +GANSTVV+AG
Sbjct: 241 GAQARHRIFHEELVVKTFFEAEKAHRGQTRASGDPYLQHCVETAVLLANIGANSTVVSAG 300

Query: 301 LLHDTLDDSFVSHDYILGTFGAGVADLVEGVSKLSHLSKLAREHDTADRMVEADRLHTMF 360
           LLHDT+DDSF+ +D+I   FGAGVADLVEGVSKLSHLSKLAR+++TA R+VEADRLHTM 
Sbjct: 301 LLHDTIDDSFIDYDHIFHMFGAGVADLVEGVSKLSHLSKLARDNNTASRIVEADRLHTML 360

Query: 361 LAMADARAVLIKLADRLHNMMTLDALPLIKRQRFAKETMEIFVPLANRLGIYSWKEQLEN 420
           LAMADARAVLIKLADR+HNM TL+ALPL K+QRFAKETMEIFVPLANRLGI SWK+QLEN
Sbjct: 361 LAMADARAVLIKLADRVHNMKTLEALPLGKQQRFAKETMEIFVPLANRLGIASWKDQLEN 420

Query: 421 LCFKHLNLEQHKDLSSKLMGLYDEAIIYSAIQKLERALEDKGISYHVVTGRHKSVYSVHC 480
           LCFKHLN E+HKDLSSKL   +DE +I SA+ KL+R L D G+SYH ++GRHKS+YS+H 
Sbjct: 421 LCFKHLNPEEHKDLSSKLTKSFDEVLITSAVDKLDRGLRDAGLSYHNLSGRHKSLYSIHN 480

Query: 481 KMLKKNLTMNEIHDIHGLRLIVEKEEDCYEALRIVHQLWPEVPGKLKDYISKPKLNGYFS 540
           KMLKKNLTM+EIHDIHGLRL+ EKEEDCY AL +VH+LWP+VPG+ KDYIS+PKLNG   
Sbjct: 481 KMLKKNLTMDEIHDIHGLRLVFEKEEDCYRALDVVHELWPQVPGRFKDYISRPKLNG--- 540

Query: 541 HCSFYCFISYIFLLLYNLMVNRYQSIHTVVRGEGDIPLEVQIRTKKMHLQAEFGFAAHWR 600
                                 Y+S+HTVV  E   P EVQIRTK+MHLQAE+GFAAHWR
Sbjct: 541 ----------------------YRSLHTVVMSENVHPFEVQIRTKEMHLQAEYGFAAHWR 600

Query: 601 YKEGDSKHSSFVLQMVEWARWVLTWHCETMSKDR-SSITYADSVKPPCKFPFHSSDCSYS 660
           YKEG  +H SFVLQMVEWARWVLTW CE M+K+R +S+  +D+++PPC FP HS DC YS
Sbjct: 601 YKEGTCRH-SFVLQMVEWARWVLTWQCEAMNKERPASLGDSDAIRPPCPFPMHSEDCPYS 660

Query: 661 YKPHCVQDGPLFVIMIENEKMSVQEFPANATMMDLLERAGRGSTRWTHYRFPMKEELRPR 720
           Y   C  DGP+FVI++E++KMSVQEF AN+T+M+L++R G  + RW+ YR PMKE+LRP+
Sbjct: 661 YTRQCDHDGPIFVILLEHDKMSVQEFQANSTVMNLMDRVGTNTPRWSPYRIPMKEDLRPK 701

Query: 721 LNHKPVSDPKCKLKMGDVVELTPPIPDKSLIEYREEIQRMYE-GGFTVAT 754
           +NH+P+SD   KL MGDVVELTP +P +SL  YREEIQRMY+ GGF +AT
Sbjct: 721 VNHEPISDLNRKLSMGDVVELTPALPHESLPNYREEIQRMYDRGGFALAT 701

BLAST of MS016191 vs. ExPASy TrEMBL
Match: A0A6J1DFB1 (GTP diphosphokinase OS=Momordica charantia OX=3673 GN=LOC111020291 PE=3 SV=1)

HSP 1 Score: 1459.1 bits (3776), Expect = 0.0e+00
Identity = 733/761 (96.32%), Postives = 733/761 (96.32%), Query Frame = 0

Query: 1   MAVPTIALYTSPPSTICSSPHPCHMNSHASYDLEFTSRSSSLPSSTASSSQKPMIGGLSS 60
           MAVPTIALYTSPPSTICSSPHPCHMNSHASYDLEFTSRSSSLPSSTASSSQKPMIGGLSS
Sbjct: 1   MAVPTIALYTSPPSTICSSPHPCHMNSHASYDLEFTSRSSSLPSSTASSSQKPMIGGLSS 60

Query: 61  LFSSTTARHTSSSASISSGGDELGSFRHDKGEELKELSSSFRYSPSKFIGSFFNRDQSPV 120
           LFSSTTARHTSSSASISSGGDELGSFRHDKGEELKELSSSFRYSPSKFIGSFFNRDQSPV
Sbjct: 61  LFSSTTARHTSSSASISSGGDELGSFRHDKGEELKELSSSFRYSPSKFIGSFFNRDQSPV 120

Query: 121 SVFQGPVSCGSCGVGSAVRTPPLWTGRERSGDASFHGRGSSNRLFNGFVRNALGSCVDYD 180
           SVFQGPVSCGSCGVGSAVRTPPLWTGRERSGDASFHGRGSSNRLFNGFVRNALGSCVDYD
Sbjct: 121 SVFQGPVSCGSCGVGSAVRTPPLWTGRERSGDASFHGRGSSNRLFNGFVRNALGSCVDYD 180

Query: 181 SPRLEVSSDCLDVGSSALIVDELTFNMEDNIAEGNSESYAKDLLLSAQSKHKIFCDEFVI 240
           SPRLEVSSDCLDVGSSALIVDELTFNMEDNIAEGNSESYAKDLLLSAQSKHKIFCDEFVI
Sbjct: 181 SPRLEVSSDCLDVGSSALIVDELTFNMEDNIAEGNSESYAKDLLLSAQSKHKIFCDEFVI 240

Query: 241 KAFFEAEKAHRGQMRASGDPYFEHCVETAVMLALVGANSTVVAAGLLHDTLDDSFVSHDY 300
           KAFFEAEKAHRGQMRASGDPYFEHCVETAVMLALVGANSTVVAAGLLHDTLDDSFVSHDY
Sbjct: 241 KAFFEAEKAHRGQMRASGDPYFEHCVETAVMLALVGANSTVVAAGLLHDTLDDSFVSHDY 300

Query: 301 ILGTFGAGVADLVEGVSKLSHLSKLAREHDTADRMVEADRLHTMFLAMADARAVLIKLAD 360
           ILGTFGAGVADLVEGVSKLSHLSKLAREHDTADRMVEADRLHTMFLAMADARAVLIKLAD
Sbjct: 301 ILGTFGAGVADLVEGVSKLSHLSKLAREHDTADRMVEADRLHTMFLAMADARAVLIKLAD 360

Query: 361 RLHNMMTLDALPLIKRQRFAKETMEIFVPLANRLGIYSWKEQLENLCFKHLNLEQHKDLS 420
            LHNMMTLDALPLIKRQRFAKETMEIFVPLANRLGIYSWKEQLENLCFKHLNLEQHKDLS
Sbjct: 361 XLHNMMTLDALPLIKRQRFAKETMEIFVPLANRLGIYSWKEQLENLCFKHLNLEQHKDLS 420

Query: 421 SKLMGLYDEAIIYSAIQKLERALEDKGISYHVVTGRHKSVYSVHCKMLKKNLTMNEIHDI 480
           SKLMGLYDEAIIYSAIQKLERALEDKGISYHVVTGRHKSVYSVHCKMLKKNLTMNEIHDI
Sbjct: 421 SKLMGLYDEAIIYSAIQKLERALEDKGISYHVVTGRHKSVYSVHCKMLKKNLTMNEIHDI 480

Query: 481 HGLRLIVEKEEDCYEALRIVHQLWPEVPGKLKDYISKPKLNGYFSHCSFYCFISYIFLLL 540
           HGLRLIVEKEEDCYEALRIVHQLWPEV GKLKDYISKPKLNG                  
Sbjct: 481 HGLRLIVEKEEDCYEALRIVHQLWPEVLGKLKDYISKPKLNG------------------ 540

Query: 541 YNLMVNRYQSIHTVVRGEGDIPLEVQIRTKKMHLQAEFGFAAHWRYKEGDSKHSSFVLQM 600
                  YQSIHTVVRGEGDIPLEVQIRTKKMHLQAEFGFAAHWRYKEGDSKHSSFVLQM
Sbjct: 541 -------YQSIHTVVRGEGDIPLEVQIRTKKMHLQAEFGFAAHWRYKEGDSKHSSFVLQM 600

Query: 601 VEWARWVLTWHCETMSKDRSSITYADSVKPPCKFPFHSSDCSYSYKPHCVQDGPLFVIMI 660
           VEWARWVLTWHCETMSKDRSSITYADSVKPPCK PFHSSDCSYSYKPHCVQDGPLFVIMI
Sbjct: 601 VEWARWVLTWHCETMSKDRSSITYADSVKPPCKLPFHSSDCSYSYKPHCVQDGPLFVIMI 660

Query: 661 ENEKMSVQEFPANATMMDLLERAGRGSTRWTHYRFPMKEELRPRLNHKPVSDPKCKLKMG 720
           ENEKMSVQEFPANATMMDLLERAGRGSTRWTHYRFPMKEELRPRLNHKPVSDPKCKLKMG
Sbjct: 661 ENEKMSVQEFPANATMMDLLERAGRGSTRWTHYRFPMKEELRPRLNHKPVSDPKCKLKMG 720

Query: 721 DVVELTPPIPDKSLIEYREEIQRMYEGGFTVATPEPAGWSS 762
           DVVELTPPIPDKSLIEYREEIQRMYEGGFTVATPEPAGWSS
Sbjct: 721 DVVELTPPIPDKSLIEYREEIQRMYEGGFTVATPEPAGWSS 736

BLAST of MS016191 vs. ExPASy TrEMBL
Match: A0A6J1GVE8 (GTP diphosphokinase OS=Cucurbita moschata OX=3662 GN=LOC111457535 PE=3 SV=1)

HSP 1 Score: 1340.5 bits (3468), Expect = 0.0e+00
Identity = 671/761 (88.17%), Postives = 701/761 (92.12%), Query Frame = 0

Query: 1   MAVPTIALYTSPPSTICSSPHPCHMNSHASYDLEFTSRSSSLPSSTASSSQKPMIGGLSS 60
           MAVPTIALYTSPPSTICSSPHPC MN+HAS DL+FTSRSSSL SSTA+SSQKPM+GGLSS
Sbjct: 1   MAVPTIALYTSPPSTICSSPHPCQMNAHASCDLDFTSRSSSLASSTATSSQKPMVGGLSS 60

Query: 61  LFSSTTARHTSSSASISSGGDELGSFRHDKGEELKELSSSFRYSPSKFIGSFFNRDQSPV 120
           LFSST  RH+SSS+SISSGG+ELGSF+HDKGEELKELSSSFRYSP+KFIGSFFNRDQSPV
Sbjct: 61  LFSSTALRHSSSSSSISSGGEELGSFKHDKGEELKELSSSFRYSPNKFIGSFFNRDQSPV 120

Query: 121 SVFQGPVSCGSCGVGSAVRTPPLWTGRERSGDASFHGRGSSNRLFNGFVRNALGSCVDYD 180
           SVFQGPVSCGSCGVGSA RTPPLWT RERSGD SFHGRGS+NRLFNGFVRNALGSCVDYD
Sbjct: 121 SVFQGPVSCGSCGVGSAARTPPLWTVRERSGDGSFHGRGSTNRLFNGFVRNALGSCVDYD 180

Query: 181 SPRLEVSSDCLDVGSSALIVDELTFNMEDNIAEGNSESYAKDLLLSAQSKHKIFCDEFVI 240
           SPRLEVSSD LD  SSAL  DELTFNMED+I EGNS+SY KDLL SAQSKHKIFCDEFVI
Sbjct: 181 SPRLEVSSDRLDASSSALFGDELTFNMEDSITEGNSKSYVKDLLQSAQSKHKIFCDEFVI 240

Query: 241 KAFFEAEKAHRGQMRASGDPYFEHCVETAVMLALVGANSTVVAAGLLHDTLDDSFVSHDY 300
           KAF EAEKAHRGQMRASGDPY EHCVETAVMLALVGANSTVVAAGLLHDTLDDSF+SHDY
Sbjct: 241 KAFNEAEKAHRGQMRASGDPYLEHCVETAVMLALVGANSTVVAAGLLHDTLDDSFLSHDY 300

Query: 301 ILGTFGAGVADLVEGVSKLSHLSKLAREHDTADRMVEADRLHTMFLAMADARAVLIKLAD 360
           IL TFG  VADLVEGVSKLSHLSKLAREHDTA+R+VEADRLHTMFLAMADARAVLIKLAD
Sbjct: 301 ILETFGVEVADLVEGVSKLSHLSKLAREHDTAERIVEADRLHTMFLAMADARAVLIKLAD 360

Query: 361 RLHNMMTLDALPLIKRQRFAKETMEIFVPLANRLGIYSWKEQLENLCFKHLNLEQHKDLS 420
           RLHNMMTLDALPLIKRQRFAKETMEIFVPLANRLGIYSWKEQLENLCFKHLNLE H+DLS
Sbjct: 361 RLHNMMTLDALPLIKRQRFAKETMEIFVPLANRLGIYSWKEQLENLCFKHLNLELHEDLS 420

Query: 421 SKLMGLYDEAIIYSAIQKLERALEDKGISYHVVTGRHKSVYSVHCKMLKKNLTMNEIHDI 480
           SKL+GLYDEAII+SAIQKLERAL+D GISYHVVTGRHKSVYS+H KMLKKNLT+NEIHDI
Sbjct: 421 SKLLGLYDEAIIFSAIQKLERALKDNGISYHVVTGRHKSVYSIHRKMLKKNLTVNEIHDI 480

Query: 481 HGLRLIVEKEEDCYEALRIVHQLWPEVPGKLKDYISKPKLNGYFSHCSFYCFISYIFLLL 540
           HGLRLIVE EEDC+EALRIV QLWPEVPGKLKDYISKPKLNG                  
Sbjct: 481 HGLRLIVENEEDCHEALRIVRQLWPEVPGKLKDYISKPKLNG------------------ 540

Query: 541 YNLMVNRYQSIHTVVRGEGDIPLEVQIRTKKMHLQAEFGFAAHWRYKEGDSKHSSFVLQM 600
                  YQSIHTVVRGEGD+PLEVQIRTK+MHLQAEFGFAAHWRYKEGDSKHSSFVLQM
Sbjct: 541 -------YQSIHTVVRGEGDVPLEVQIRTKEMHLQAEFGFAAHWRYKEGDSKHSSFVLQM 600

Query: 601 VEWARWVLTWHCETMSKDRSSITYADSVKPPCKFPFHSSDCSYSYKPHCVQDGPLFVIMI 660
           VEWARWVLTWHCETM++DRSSI    S+KPPC FPFHSSDCSYSYKPHC QDGPLFVIMI
Sbjct: 601 VEWARWVLTWHCETMNRDRSSI---GSIKPPCNFPFHSSDCSYSYKPHCFQDGPLFVIMI 660

Query: 661 ENEKMSVQEFPANATMMDLLERAGRGSTRWTHYRFPMKEELRPRLNHKPVSDPKCKLKMG 720
           ENEKMSVQEFPAN TMMDLLERAGRGSTRW HYRFP+KEELRPRLNH+PVSDPKCKLKMG
Sbjct: 661 ENEKMSVQEFPANTTMMDLLERAGRGSTRWAHYRFPIKEELRPRLNHEPVSDPKCKLKMG 720

Query: 721 DVVELTPPIPDKSLIEYREEIQRMYEGGFTVATPEPAGWSS 762
           DVVELTPPIPDKSLIEYREEIQRMYEGGFTVATP+PAGW S
Sbjct: 721 DVVELTPPIPDKSLIEYREEIQRMYEGGFTVATPQPAGWRS 733

BLAST of MS016191 vs. ExPASy TrEMBL
Match: A0A0A0L9B1 (GTP diphosphokinase OS=Cucumis sativus OX=3659 GN=Csa_3G651840 PE=3 SV=1)

HSP 1 Score: 1338.9 bits (3464), Expect = 0.0e+00
Identity = 669/761 (87.91%), Postives = 701/761 (92.12%), Query Frame = 0

Query: 1   MAVPTIALYTSPPSTICSSPHPCHMNSHASYDLEFTSRSSSLPSSTASSSQKPMIGGLSS 60
           MAVPTIA YTSPPSTICSSPHPC +N+HAS DLEFTSRSSSL SSTA+SSQKPM+GGLSS
Sbjct: 1   MAVPTIAFYTSPPSTICSSPHPCQINTHASCDLEFTSRSSSLASSTAASSQKPMVGGLSS 60

Query: 61  LFSSTTARHTSSSASISSGGDELGSFRHDKGEELKELSSSFRYSPSKFIGSFFNRDQSPV 120
           LFSST  R +SSSASISSGGDELGSFRHDKG+ELKELSSSFRYSP+KFIGSFFNRDQSPV
Sbjct: 61  LFSSTAPRLSSSSASISSGGDELGSFRHDKGDELKELSSSFRYSPNKFIGSFFNRDQSPV 120

Query: 121 SVFQGPVSCGSCGVGSAVRTPPLWTGRERSGDASFHGRGSSNRLFNGFVRNALGSCVDYD 180
           SVFQGPVSCGSCG GSA RTPPLWT RERSGD SFHGRG +NRLF+GFVRNALGSCVDYD
Sbjct: 121 SVFQGPVSCGSCGFGSAARTPPLWTVRERSGDGSFHGRGGTNRLFSGFVRNALGSCVDYD 180

Query: 181 SPRLEVSSDCLDVGSSALIVDELTFNMEDNIAEGNSESYAKDLLLSAQSKHKIFCDEFVI 240
           SPRLEVSSD LDVGSSAL  DELTFNMEDNI EGNSESYAKDLLLSAQSKHKIFCDEFV+
Sbjct: 181 SPRLEVSSDGLDVGSSALFGDELTFNMEDNITEGNSESYAKDLLLSAQSKHKIFCDEFVV 240

Query: 241 KAFFEAEKAHRGQMRASGDPYFEHCVETAVMLALVGANSTVVAAGLLHDTLDDSFVSHDY 300
           KAFFEAEKAHRGQ+RASGDPY EHCVETAVMLALVGANSTVVAAGLLHDT+DDSFV+HDY
Sbjct: 241 KAFFEAEKAHRGQLRASGDPYLEHCVETAVMLALVGANSTVVAAGLLHDTIDDSFVTHDY 300

Query: 301 ILGTFGAGVADLVEGVSKLSHLSKLAREHDTADRMVEADRLHTMFLAMADARAVLIKLAD 360
           ILGTFGA VADLVEGVSKLSHLSKLAREHDTA+R VEADRLHTMFLAMADARAVL+KLAD
Sbjct: 301 ILGTFGAEVADLVEGVSKLSHLSKLAREHDTAERTVEADRLHTMFLAMADARAVLVKLAD 360

Query: 361 RLHNMMTLDALPLIKRQRFAKETMEIFVPLANRLGIYSWKEQLENLCFKHLNLEQHKDLS 420
           RLHNMMTLDALP IK+QRFAKETMEIFVPLANRLGIY+WKEQLEN+CFKHLNLEQH+DLS
Sbjct: 361 RLHNMMTLDALPPIKQQRFAKETMEIFVPLANRLGIYTWKEQLENMCFKHLNLEQHEDLS 420

Query: 421 SKLMGLYDEAIIYSAIQKLERALEDKGISYHVVTGRHKSVYSVHCKMLKKNLTMNEIHDI 480
           SKL+GLYDEAII+SA QKLERAL+DKGISYHVVTGRHKSVYS+H KMLKKNLT+NEIHDI
Sbjct: 421 SKLLGLYDEAIIFSATQKLERALKDKGISYHVVTGRHKSVYSIHRKMLKKNLTVNEIHDI 480

Query: 481 HGLRLIVEKEEDCYEALRIVHQLWPEVPGKLKDYISKPKLNGYFSHCSFYCFISYIFLLL 540
           HGLRLIVE EEDCYEALRIVHQLWP VPGKLKDYISKPKLNG                  
Sbjct: 481 HGLRLIVENEEDCYEALRIVHQLWPNVPGKLKDYISKPKLNG------------------ 540

Query: 541 YNLMVNRYQSIHTVVRGEGDIPLEVQIRTKKMHLQAEFGFAAHWRYKEGDSKHSSFVLQM 600
                  YQSIHTVVRGEGD+PLEVQIRTK+MHLQAEFGFAAHWRYKEGDSKHSSFVLQM
Sbjct: 541 -------YQSIHTVVRGEGDVPLEVQIRTKEMHLQAEFGFAAHWRYKEGDSKHSSFVLQM 600

Query: 601 VEWARWVLTWHCETMSKDRSSITYADSVKPPCKFPFHSSDCSYSYKPHCVQDGPLFVIMI 660
           VEWARWVLTWHCETM+KDR SI    SV+PPCKFPFHSSDCSYSYKP   QDGPLFVIMI
Sbjct: 601 VEWARWVLTWHCETMNKDRPSI---GSVRPPCKFPFHSSDCSYSYKPRYFQDGPLFVIMI 660

Query: 661 ENEKMSVQEFPANATMMDLLERAGRGSTRWTHYRFPMKEELRPRLNHKPVSDPKCKLKMG 720
           ENEKMSVQEFPA+ATMMDLLERAGRGSTRW HYRFPMKEELRPRLNH+PVSDPKCKLKMG
Sbjct: 661 ENEKMSVQEFPADATMMDLLERAGRGSTRWAHYRFPMKEELRPRLNHEPVSDPKCKLKMG 720

Query: 721 DVVELTPPIPDKSLIEYREEIQRMYEGGFTVATPEPAGWSS 762
           DVVELTPPIPDK L+EYREEIQRMYEGGFTVATP+PAGW S
Sbjct: 721 DVVELTPPIPDKLLVEYREEIQRMYEGGFTVATPQPAGWKS 733

BLAST of MS016191 vs. ExPASy TrEMBL
Match: A0A1S4E4I0 (GTP diphosphokinase OS=Cucumis melo OX=3656 GN=LOC103501986 PE=3 SV=1)

HSP 1 Score: 1337.0 bits (3459), Expect = 0.0e+00
Identity = 668/761 (87.78%), Postives = 700/761 (91.98%), Query Frame = 0

Query: 1   MAVPTIALYTSPPSTICSSPHPCHMNSHASYDLEFTSRSSSLPSSTASSSQKPMIGGLSS 60
           MAVPTIALYTSPPSTICSSPHPC +NSHAS DLEFTSRSSSL SSTA+SSQKPM+GGLSS
Sbjct: 1   MAVPTIALYTSPPSTICSSPHPCQINSHASCDLEFTSRSSSLASSTAASSQKPMVGGLSS 60

Query: 61  LFSSTTARHTSSSASISSGGDELGSFRHDKGEELKELSSSFRYSPSKFIGSFFNRDQSPV 120
           LFSS+  RH+SSSASISSGGDELGSFRHDKG+ELKE SSSFRYSP+KFIGSFFNRDQSPV
Sbjct: 61  LFSSSAPRHSSSSASISSGGDELGSFRHDKGDELKEFSSSFRYSPNKFIGSFFNRDQSPV 120

Query: 121 SVFQGPVSCGSCGVGSAVRTPPLWTGRERSGDASFHGRGSSNRLFNGFVRNALGSCVDYD 180
           SVFQGPVSCGSCG+GSA RTPPLWT RERSGD SFHGRGS+NRLF+GF RNALGSCVDYD
Sbjct: 121 SVFQGPVSCGSCGLGSASRTPPLWTVRERSGDGSFHGRGSTNRLFSGFARNALGSCVDYD 180

Query: 181 SPRLEVSSDCLDVGSSALIVDELTFNMEDNIAEGNSESYAKDLLLSAQSKHKIFCDEFVI 240
           SPRLEVSSD LDVGSSAL  DELTFNMEDNI EGNSESYAKDLLLSAQSKH+IFCDEFV+
Sbjct: 181 SPRLEVSSDGLDVGSSALFGDELTFNMEDNITEGNSESYAKDLLLSAQSKHRIFCDEFVV 240

Query: 241 KAFFEAEKAHRGQMRASGDPYFEHCVETAVMLALVGANSTVVAAGLLHDTLDDSFVSHDY 300
           KAFFEAEKAHRGQMRASGDPY EHCVETAVMLALVGANSTVVAAGLLHDT+DDSFV+HDY
Sbjct: 241 KAFFEAEKAHRGQMRASGDPYLEHCVETAVMLALVGANSTVVAAGLLHDTIDDSFVTHDY 300

Query: 301 ILGTFGAGVADLVEGVSKLSHLSKLAREHDTADRMVEADRLHTMFLAMADARAVLIKLAD 360
           ILGTFGA VADLVEGVSKLSHLSKLAREHDTA+RMVEADRLHTMFLAMADARAVL+KLAD
Sbjct: 301 ILGTFGAEVADLVEGVSKLSHLSKLAREHDTAERMVEADRLHTMFLAMADARAVLVKLAD 360

Query: 361 RLHNMMTLDALPLIKRQRFAKETMEIFVPLANRLGIYSWKEQLENLCFKHLNLEQHKDLS 420
           RLHNMMTLDALP IKRQRFAKETMEIFVPLANRLGIY+WKEQLEN+CFKHLNLEQH+DLS
Sbjct: 361 RLHNMMTLDALPPIKRQRFAKETMEIFVPLANRLGIYTWKEQLENMCFKHLNLEQHEDLS 420

Query: 421 SKLMGLYDEAIIYSAIQKLERALEDKGISYHVVTGRHKSVYSVHCKMLKKNLTMNEIHDI 480
           SKL+GLYDEAII SA QKLERAL+DKG SYH VTGRHKSVYS+H KMLKKNLT+NEIHDI
Sbjct: 421 SKLLGLYDEAIILSATQKLERALKDKGTSYHDVTGRHKSVYSIHRKMLKKNLTVNEIHDI 480

Query: 481 HGLRLIVEKEEDCYEALRIVHQLWPEVPGKLKDYISKPKLNGYFSHCSFYCFISYIFLLL 540
           HGLRLIVE EEDCYEALRIVHQLWP +PGKLKDYISKPKLNG                  
Sbjct: 481 HGLRLIVENEEDCYEALRIVHQLWPNIPGKLKDYISKPKLNG------------------ 540

Query: 541 YNLMVNRYQSIHTVVRGEGDIPLEVQIRTKKMHLQAEFGFAAHWRYKEGDSKHSSFVLQM 600
                  YQSIHTVVRGEGD+PLEVQIRTK+MHLQAEFGFAAHWRYKE DSKHSSFVLQM
Sbjct: 541 -------YQSIHTVVRGEGDVPLEVQIRTKEMHLQAEFGFAAHWRYKESDSKHSSFVLQM 600

Query: 601 VEWARWVLTWHCETMSKDRSSITYADSVKPPCKFPFHSSDCSYSYKPHCVQDGPLFVIMI 660
           VEWARWVLTWHCETM+KDR SI    SVKPPCKFPFHSSDCSYSYKP   QDGPLFVIMI
Sbjct: 601 VEWARWVLTWHCETMNKDRPSI---GSVKPPCKFPFHSSDCSYSYKPRYFQDGPLFVIMI 660

Query: 661 ENEKMSVQEFPANATMMDLLERAGRGSTRWTHYRFPMKEELRPRLNHKPVSDPKCKLKMG 720
           ENEKMSVQEFPA+ATMMDLLERAGRGSTRW HYRFPMKEELRPRLNH+PVSDPKCKLKMG
Sbjct: 661 ENEKMSVQEFPADATMMDLLERAGRGSTRWAHYRFPMKEELRPRLNHEPVSDPKCKLKMG 720

Query: 721 DVVELTPPIPDKSLIEYREEIQRMYEGGFTVATPEPAGWSS 762
           DVVEL+PPIPDK L+EYREEIQRMYEGGFTVATP+PAGW S
Sbjct: 721 DVVELSPPIPDKLLVEYREEIQRMYEGGFTVATPQPAGWRS 733

BLAST of MS016191 vs. ExPASy TrEMBL
Match: A0A5D3CA85 (GTP diphosphokinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold606G001080 PE=3 SV=1)

HSP 1 Score: 1337.0 bits (3459), Expect = 0.0e+00
Identity = 668/761 (87.78%), Postives = 700/761 (91.98%), Query Frame = 0

Query: 1   MAVPTIALYTSPPSTICSSPHPCHMNSHASYDLEFTSRSSSLPSSTASSSQKPMIGGLSS 60
           MAVPTIALYTSPPSTICSSPHPC +NSHAS DLEFTSRSSSL SSTA+SSQKPM+GGLSS
Sbjct: 1   MAVPTIALYTSPPSTICSSPHPCQINSHASCDLEFTSRSSSLASSTAASSQKPMVGGLSS 60

Query: 61  LFSSTTARHTSSSASISSGGDELGSFRHDKGEELKELSSSFRYSPSKFIGSFFNRDQSPV 120
           LFSS+  RH+SSSASISSGGDELGSFRHDKG+ELKE SSSFRYSP+KFIGSFFNRDQSPV
Sbjct: 61  LFSSSAPRHSSSSASISSGGDELGSFRHDKGDELKEFSSSFRYSPNKFIGSFFNRDQSPV 120

Query: 121 SVFQGPVSCGSCGVGSAVRTPPLWTGRERSGDASFHGRGSSNRLFNGFVRNALGSCVDYD 180
           SVFQGPVSCGSCG+GSA RTPPLWT RERSGD SFHGRGS+NRLF+GF RNALGSCVDYD
Sbjct: 121 SVFQGPVSCGSCGLGSASRTPPLWTVRERSGDGSFHGRGSTNRLFSGFARNALGSCVDYD 180

Query: 181 SPRLEVSSDCLDVGSSALIVDELTFNMEDNIAEGNSESYAKDLLLSAQSKHKIFCDEFVI 240
           SPRLEVSSD LDVGSSAL  DELTFNMEDNI EGNSESYAKDLLLSAQSKH+IFCDEFV+
Sbjct: 181 SPRLEVSSDGLDVGSSALFGDELTFNMEDNITEGNSESYAKDLLLSAQSKHRIFCDEFVV 240

Query: 241 KAFFEAEKAHRGQMRASGDPYFEHCVETAVMLALVGANSTVVAAGLLHDTLDDSFVSHDY 300
           KAFFEAEKAHRGQMRASGDPY EHCVETAVMLALVGANSTVVAAGLLHDT+DDSFV+HDY
Sbjct: 241 KAFFEAEKAHRGQMRASGDPYLEHCVETAVMLALVGANSTVVAAGLLHDTIDDSFVTHDY 300

Query: 301 ILGTFGAGVADLVEGVSKLSHLSKLAREHDTADRMVEADRLHTMFLAMADARAVLIKLAD 360
           ILGTFGA VADLVEGVSKLSHLSKLAREHDTA+RMVEADRLHTMFLAMADARAVL+KLAD
Sbjct: 301 ILGTFGAEVADLVEGVSKLSHLSKLAREHDTAERMVEADRLHTMFLAMADARAVLVKLAD 360

Query: 361 RLHNMMTLDALPLIKRQRFAKETMEIFVPLANRLGIYSWKEQLENLCFKHLNLEQHKDLS 420
           RLHNMMTLDALP IKRQRFAKETMEIFVPLANRLGIY+WKEQLEN+CFKHLNLEQH+DLS
Sbjct: 361 RLHNMMTLDALPPIKRQRFAKETMEIFVPLANRLGIYTWKEQLENMCFKHLNLEQHEDLS 420

Query: 421 SKLMGLYDEAIIYSAIQKLERALEDKGISYHVVTGRHKSVYSVHCKMLKKNLTMNEIHDI 480
           SKL+GLYDEAII SA QKLERAL+DKG SYH VTGRHKSVYS+H KMLKKNLT+NEIHDI
Sbjct: 421 SKLLGLYDEAIILSATQKLERALKDKGTSYHDVTGRHKSVYSIHRKMLKKNLTVNEIHDI 480

Query: 481 HGLRLIVEKEEDCYEALRIVHQLWPEVPGKLKDYISKPKLNGYFSHCSFYCFISYIFLLL 540
           HGLRLIVE EEDCYEALRIVHQLWP +PGKLKDYISKPKLNG                  
Sbjct: 481 HGLRLIVENEEDCYEALRIVHQLWPNIPGKLKDYISKPKLNG------------------ 540

Query: 541 YNLMVNRYQSIHTVVRGEGDIPLEVQIRTKKMHLQAEFGFAAHWRYKEGDSKHSSFVLQM 600
                  YQSIHTVVRGEGD+PLEVQIRTK+MHLQAEFGFAAHWRYKE DSKHSSFVLQM
Sbjct: 541 -------YQSIHTVVRGEGDVPLEVQIRTKEMHLQAEFGFAAHWRYKESDSKHSSFVLQM 600

Query: 601 VEWARWVLTWHCETMSKDRSSITYADSVKPPCKFPFHSSDCSYSYKPHCVQDGPLFVIMI 660
           VEWARWVLTWHCETM+KDR SI    SVKPPCKFPFHSSDCSYSYKP   QDGPLFVIMI
Sbjct: 601 VEWARWVLTWHCETMNKDRPSI---GSVKPPCKFPFHSSDCSYSYKPRYFQDGPLFVIMI 660

Query: 661 ENEKMSVQEFPANATMMDLLERAGRGSTRWTHYRFPMKEELRPRLNHKPVSDPKCKLKMG 720
           ENEKMSVQEFPA+ATMMDLLERAGRGSTRW HYRFPMKEELRPRLNH+PVSDPKCKLKMG
Sbjct: 661 ENEKMSVQEFPADATMMDLLERAGRGSTRWAHYRFPMKEELRPRLNHEPVSDPKCKLKMG 720

Query: 721 DVVELTPPIPDKSLIEYREEIQRMYEGGFTVATPEPAGWSS 762
           DVVEL+PPIPDK L+EYREEIQRMYEGGFTVATP+PAGW S
Sbjct: 721 DVVELSPPIPDKLLVEYREEIQRMYEGGFTVATPQPAGWRS 733

BLAST of MS016191 vs. TAIR 10
Match: AT3G14050.1 (RELA/SPOT homolog 2 )

HSP 1 Score: 922.5 bits (2383), Expect = 2.1e-268
Identity = 494/762 (64.83%), Postives = 581/762 (76.25%), Query Frame = 0

Query: 5   TIALYTSPPSTICSSPHPCHMNSHASYDLEFTSRSSSLPSSTASSSQKPMIGGLSSLFSS 64
           TIALY SPPS++CS+PH        S DL+ TSRSSS  SS ASS QKP++GGLSSLFSS
Sbjct: 6   TIALYASPPSSVCSTPH------QISCDLDLTSRSSSTSSSMASSPQKPIVGGLSSLFSS 65

Query: 65  TTARHTSSSA-SISSGGDELGSFRHDKGEELKEL--SSSFRYSPSKFIGSFFNRDQSPVS 124
            + + +SSS+ S S+G DE  S R+D+ ++LK+L  SSSF YSP+KF+ SF    QSP+S
Sbjct: 66  ASVKSSSSSSCSYSTGVDEFSSLRYDRSDDLKDLSFSSSFGYSPAKFVNSFRRDHQSPIS 125

Query: 125 VFQGPVSCGSCGVGSAVRTPPLWTGRERSGDASFHGRGSSNRLFNGFVRNALGSCVDYDS 184
           V  GPVSC SC       +PP+   R+R+ D SF  R  ++ LFNGFVR ALGSCVDY  
Sbjct: 126 VLHGPVSC-SC-------SPPMRMSRDRNLDGSF--RLGASGLFNGFVRKALGSCVDY-- 185

Query: 185 PRLEVSSDCLDVGSSALIVDELTFNME-DNIAEGNSESYAKDLLLSAQSKHKIFCDEFVI 244
                     + GS +++VDELTF ME D I     + YA+DLL  AQ +HKIF DE VI
Sbjct: 186 ----------EFGSDSVLVDELTFPMEVDTI-----KPYARDLLRRAQLRHKIFNDESVI 245

Query: 245 KAFFEAEKAHRGQMRASGDPYFEHCVETAVMLALVGANSTVVAAGLLHDTLDDSFVSHDY 304
           KAF+EAEKAHRGQMRAS DPY +HCVETA++LA +GANSTVV AGLLHDT+DDSF+S+DY
Sbjct: 246 KAFYEAEKAHRGQMRASRDPYLQHCVETAMLLANIGANSTVVVAGLLHDTIDDSFMSYDY 305

Query: 305 ILGTFGAGVADLVEGVSKLSHLSKLAREHDTADRMVEADRLHTMFLAMADARAVLIKLAD 364
           IL  FGAGVADLVEGVSKLS LSKLARE++TA + VEADRLHTMFLAMADARAVLIKLAD
Sbjct: 306 ILRNFGAGVADLVEGVSKLSQLSKLARENNTACKTVEADRLHTMFLAMADARAVLIKLAD 365

Query: 365 RLHNMMTLDALPLIKRQRFAKETMEIFVPLANRLGIYSWKEQLENLCFKHLNLEQHKDLS 424
           RLHNM TL AL  +K+QRFAKET+EIF PLANRLGI +WK QLENLCFKHL   QH ++S
Sbjct: 366 RLHNMKTLYALSPVKQQRFAKETLEIFAPLANRLGISTWKVQLENLCFKHLYPNQHNEMS 425

Query: 425 SKLMGLYDEAIIYSAIQKLERALEDKGISYHVVTGRHKSVYSVHCKMLKKNLTMNEIHDI 484
           + L   +DEA+I SAI+KLE+AL+  GISYHV+ GRHKS+YS++ KMLKK LT++EIHDI
Sbjct: 426 TMLEDSFDEAMITSAIEKLEQALKKAGISYHVLCGRHKSLYSIYSKMLKKKLTVDEIHDI 485

Query: 485 HGLRLIVEKEEDCYEALRIVHQLWPEVPGKLKDYISKPKLNGYFSHCSFYCFISYIFLLL 544
           HGLRLIV+ E DCY+AL +VH LW EVPGKLKDYI+ PK NG                  
Sbjct: 486 HGLRLIVDNEGDCYKALGVVHSLWSEVPGKLKDYITHPKFNG------------------ 545

Query: 545 YNLMVNRYQSIHTVVRGEGDIPLEVQIRTKKMHLQAEFGFAAHWRYKEGDSKHSSFVLQM 604
                  YQS+HTVV   G +PLEVQIRT++MHLQAEFGFAAHWRYKEG  K+SSFVLQM
Sbjct: 546 -------YQSLHTVVMDNGTVPLEVQIRTQEMHLQAEFGFAAHWRYKEGGCKYSSFVLQM 605

Query: 605 VEWARWVLTWHCETMSKDRSSITYADSVKPPCKFPFHSSDCSYSYKPHCVQDGPLFVIMI 664
           VEWARWV+TWHCE MSKDRSSI+ +DS+KPPCKFP HS DC  SYKP+  QDGP++VI+I
Sbjct: 606 VEWARWVVTWHCEAMSKDRSSISSSDSIKPPCKFPSHSEDCPASYKPNSSQDGPVYVIVI 665

Query: 665 ENEKMSVQEFPANATMMDLLERAGRGSTRWTHYRFPMKEELRPRLNHKPVSDPKCKLKMG 724
           EN+KMSVQEFPA++T+ DLL RAG GS+RW+ Y  P KEELRPRLN  PVSD K KLKMG
Sbjct: 666 ENDKMSVQEFPASSTVSDLLSRAGPGSSRWSMYGIPAKEELRPRLNQIPVSDLKWKLKMG 709

Query: 725 DVVELTPPIPDKSLIEYREEIQRMYEGGFTVATP-EPAGWSS 762
           DVVELTP IPD+SL EYREEIQRMY+ G   + P    GW S
Sbjct: 726 DVVELTPTIPDESLTEYREEIQRMYDRGLAFSRPGTMVGWGS 709

BLAST of MS016191 vs. TAIR 10
Match: AT1G54130.1 (RELA/SPOT homolog 3 )

HSP 1 Score: 919.8 bits (2376), Expect = 1.4e-267
Identity = 494/766 (64.49%), Postives = 578/766 (75.46%), Query Frame = 0

Query: 5   TIALYTSPPSTICSSPHPCHMNSHASYDLEFTSRSSSLPSSTASSSQKPMIGGLSSLFSS 64
           TIALY SP ST+CS+ H   +N+H S DL+  SRSSS  SST+S    P IGGLS LFS 
Sbjct: 6   TIALYASPASTVCSTAH--QINAHISCDLDLNSRSSSASSSTSS----PTIGGLSLLFSG 65

Query: 65  TTARHTSSSASI-SSGGDELGSFRHDKGEELKELSSSFRYSPSKFIG-SFFNRD-QSPVS 124
            + + +SSS+S   S G+EL S RHD+ E+ + LS SF YSPSKFIG S+  RD QSPVS
Sbjct: 66  ASVKSSSSSSSSHPSVGEELASIRHDRSED-RTLSGSFCYSPSKFIGSSYLKRDHQSPVS 125

Query: 125 VFQGPVSCGSCGVGSAVRTPPLWTGRERSGDASFHGRGSSNRLFNGFVRNALGSCVDYDS 184
           V  GP+S G+        +PP+   R+R+ D     R  S+RLFNGFVR A+GSCVDYD+
Sbjct: 126 VLHGPISSGN--------SPPMRISRDRNLDGGSALRVGSSRLFNGFVRKAIGSCVDYDT 185

Query: 185 PRLEVSSDCLDVGSSALIVDELTFNMEDNIAEGNSESYAKDLLLSAQSKHKIFCDEFVIK 244
                         S L+ ++L F M+D       + YA+DLL  AQ KHKIF DE VIK
Sbjct: 186 -------------DSVLVDEQLPFTMDDGFEGERRQPYARDLLRRAQLKHKIFEDESVIK 245

Query: 245 AFFEAEKAHRGQMRASGDPYFEHCVETAVMLALVGANSTVVAAGLLHDTLDDSFVSHDYI 304
           AF+EAEKAHRGQMRA+GDPY +HCVETA++LA +GANSTVV AG+LHDTLDDSF+S+DYI
Sbjct: 246 AFYEAEKAHRGQMRATGDPYLQHCVETAMLLADIGANSTVVVAGILHDTLDDSFMSYDYI 305

Query: 305 LGTFGAGVADLVEGVSKLSHLSKLAREHDTADRMVEADRLHTMFLAMADARAVLIKLADR 364
           L TFG+GVADLVEGVSKLS LSKLARE++TA + VEADRLHTMFLAMADARAVLIKLADR
Sbjct: 306 LRTFGSGVADLVEGVSKLSQLSKLARENNTACKTVEADRLHTMFLAMADARAVLIKLADR 365

Query: 365 LHNMMTLDALPLIKRQRFAKETMEIFVPLANRLGIYSWKEQLENLCFKHLNLEQHKDLSS 424
           LHNMMTL ALP +KRQRFAKET+EIF PLANRLGI SWK +LENLCFKHL+ +QH ++S 
Sbjct: 366 LHNMMTLYALPPVKRQRFAKETLEIFAPLANRLGISSWKVKLENLCFKHLHPDQHHEMSD 425

Query: 425 KLMGLYDEAIIYSAIQKLERALEDKGISYHVVTGRHKSVYSVHCKMLKKNLTMNEIHDIH 484
            L   +DEA+I SAI+KLE+AL+ +GISYHVV+GRHKS+YS++CKMLKK LTM+EIHDIH
Sbjct: 426 MLEDSFDEAMITSAIEKLEQALKKEGISYHVVSGRHKSLYSIYCKMLKKKLTMDEIHDIH 485

Query: 485 GLRLIVEKEEDCYEALRIVHQLWPEVPGKLKDYISKPKLNGYFSHCSFYCFISYIFLLLY 544
           GLRLIV+ E+DCY+AL +VH+LW EVPGKLKDYIS PK NG                   
Sbjct: 486 GLRLIVDNEKDCYKALGVVHKLWSEVPGKLKDYISHPKFNG------------------- 545

Query: 545 NLMVNRYQSIHTVVRGEGDIPLEVQIRTKKMHLQAEFGFAAHWRYKEGDSKHSSFVLQMV 604
                 YQS+HTVV G+G IPLEVQIRTK+MHLQAEFGFAAHWRYKEGD KHSSFVLQMV
Sbjct: 546 ------YQSLHTVVMGDGTIPLEVQIRTKEMHLQAEFGFAAHWRYKEGDCKHSSFVLQMV 605

Query: 605 EWARWVLTWHCETMSKDRSSITYADSVKPPCKFPFHSSDCSYSYKPHCVQDGPLFVIMIE 664
           EWARWV+TWH ETMSKD SSI    S +P C FP H+ DC +SYKP   Q+GP++VI+IE
Sbjct: 606 EWARWVVTWHFETMSKDGSSIC---SSEPLCSFPSHAEDCPFSYKPSGNQEGPVYVIVIE 665

Query: 665 NEKMSVQEFPANATMMDLLERAGRGSTRWTHYRFPMKEELRPRLNHKPVSDPKCKLKMGD 724
           NEKM+VQEFP N+T+ DLL RAG GS+RW+ Y  P KEELRPRLN  PVSD KCKLKMGD
Sbjct: 666 NEKMTVQEFPENSTVSDLLRRAGPGSSRWSMYSIPAKEELRPRLNQTPVSDLKCKLKMGD 715

Query: 725 VVELTPPIPDKSLIEYREEIQRMYEGGFTVATPEPA------GWSS 762
           VVELTP IPDKSL EYREEIQRMY+ G   + P  A      GW S
Sbjct: 726 VVELTPAIPDKSLTEYREEIQRMYDRGLAFSRPHRAATGTMVGWGS 715

BLAST of MS016191 vs. TAIR 10
Match: AT4G02260.3 (RELA/SPOT homolog 1 )

HSP 1 Score: 198.4 bits (503), Expect = 2.1e-50
Identity = 136/376 (36.17%), Postives = 199/376 (52.93%), Query Frame = 0

Query: 237 EFVIKAFFEAEKAHRGQMRASGDPYFEHCVETAVMLALVGANSTVVAAGLLHDTLDD-SF 296
           EFV K    A +AH GQ R SG+P+  H V  A +L  +  +   + AGLLHDT++D +F
Sbjct: 148 EFVQKGLKLAFEAHHGQKRRSGEPFIIHPVAVARILGELELDWESIVAGLLHDTVEDTNF 207

Query: 297 VSHDYILGTFGAGVADLVEGVSKLSHLSKL-AREHDTADRMVEADRLHTMFLAMAD-ARA 356
           ++ + I   FGA V  +VEG +K+S L KL  +      + V+AD L  MFLAM D  R 
Sbjct: 208 ITFEKIEEEFGATVRHIVEGETKVSKLGKLKCKTESETIQDVKADDLRQMFLAMTDEVRV 267

Query: 357 VLIKLADRLHNMMTLDALPLIKRQRFAKETMEIFVPLANRLGIYSWKEQLENLCFKHLNL 416
           +++KLADRLHNM TL  +P  K+   A ET+++F PLA  LG+YS K +LENL F +++ 
Sbjct: 268 IIVKLADRLHNMRTLCHMPPHKQSSIAGETLQVFAPLAKLLGMYSIKSELENLSFMYVSA 327

Query: 417 EQHKDLSSKLMGLY--DEAIIYSAIQKLERALEDKGISYHVVTGRH-----KSVYSVHCK 476
           E +  ++S++  LY   E  +  A + L + +ED      V          K  YS++  
Sbjct: 328 EDYDRVTSRIANLYKEHEKELTEANRILVKKIEDDQFLDLVTVNTDVRSVCKETYSIYKA 387

Query: 477 MLKKNLTMNEIHDIHGLRLIVE------------KEEDCYEALRIVHQLWPEVPGKLKDY 536
            LK   ++N+ + I  LR++V+             ++ CY  L +VH++W  +P  +KDY
Sbjct: 388 ALKSKGSINDYNQIAQLRIVVKPKPSVGVGPLCSPQQICYHVLGLVHEIWKPIPRTVKDY 447

Query: 537 ISKPKLNGYFSHCSFYCFISYIFLLLYNLMVNRYQSIHTVV---RGEGDIPLEVQIRTKK 588
           I+ PK NG                         YQS+HT V     E    LEVQIRT++
Sbjct: 448 IATPKPNG-------------------------YQSLHTTVIPFLYESMFRLEVQIRTEE 498

BLAST of MS016191 vs. TAIR 10
Match: AT4G02260.2 (RELA/SPOT homolog 1 )

HSP 1 Score: 198.4 bits (503), Expect = 2.1e-50
Identity = 136/376 (36.17%), Postives = 199/376 (52.93%), Query Frame = 0

Query: 237 EFVIKAFFEAEKAHRGQMRASGDPYFEHCVETAVMLALVGANSTVVAAGLLHDTLDD-SF 296
           EFV K    A +AH GQ R SG+P+  H V  A +L  +  +   + AGLLHDT++D +F
Sbjct: 148 EFVQKGLKLAFEAHHGQKRRSGEPFIIHPVAVARILGELELDWESIVAGLLHDTVEDTNF 207

Query: 297 VSHDYILGTFGAGVADLVEGVSKLSHLSKL-AREHDTADRMVEADRLHTMFLAMAD-ARA 356
           ++ + I   FGA V  +VEG +K+S L KL  +      + V+AD L  MFLAM D  R 
Sbjct: 208 ITFEKIEEEFGATVRHIVEGETKVSKLGKLKCKTESETIQDVKADDLRQMFLAMTDEVRV 267

Query: 357 VLIKLADRLHNMMTLDALPLIKRQRFAKETMEIFVPLANRLGIYSWKEQLENLCFKHLNL 416
           +++KLADRLHNM TL  +P  K+   A ET+++F PLA  LG+YS K +LENL F +++ 
Sbjct: 268 IIVKLADRLHNMRTLCHMPPHKQSSIAGETLQVFAPLAKLLGMYSIKSELENLSFMYVSA 327

Query: 417 EQHKDLSSKLMGLY--DEAIIYSAIQKLERALEDKGISYHVVTGRH-----KSVYSVHCK 476
           E +  ++S++  LY   E  +  A + L + +ED      V          K  YS++  
Sbjct: 328 EDYDRVTSRIANLYKEHEKELTEANRILVKKIEDDQFLDLVTVNTDVRSVCKETYSIYKA 387

Query: 477 MLKKNLTMNEIHDIHGLRLIVE------------KEEDCYEALRIVHQLWPEVPGKLKDY 536
            LK   ++N+ + I  LR++V+             ++ CY  L +VH++W  +P  +KDY
Sbjct: 388 ALKSKGSINDYNQIAQLRIVVKPKPSVGVGPLCSPQQICYHVLGLVHEIWKPIPRTVKDY 447

Query: 537 ISKPKLNGYFSHCSFYCFISYIFLLLYNLMVNRYQSIHTVV---RGEGDIPLEVQIRTKK 588
           I+ PK NG                         YQS+HT V     E    LEVQIRT++
Sbjct: 448 IATPKPNG-------------------------YQSLHTTVIPFLYESMFRLEVQIRTEE 498

BLAST of MS016191 vs. TAIR 10
Match: AT4G02260.1 (RELA/SPOT homolog 1 )

HSP 1 Score: 194.5 bits (493), Expect = 3.1e-49
Identity = 136/377 (36.07%), Postives = 199/377 (52.79%), Query Frame = 0

Query: 237 EFVIKAFFEAEKAHRGQMRASGDPYFEHCVETAVMLALVGANSTVVAAGLLHDTLDD-SF 296
           EFV K    A +AH GQ R SG+P+  H V  A +L  +  +   + AGLLHDT++D +F
Sbjct: 148 EFVQKGLKLAFEAHHGQKRRSGEPFIIHPVAVARILGELELDWESIVAGLLHDTVEDTNF 207

Query: 297 VSHDYILGTFGAGVADLVEGVSKLSHLSKL-AREHDTADRMVEADRLHTMFLAMAD-ARA 356
           ++ + I   FGA V  +VEG +K+S L KL  +      + V+AD L  MFLAM D  R 
Sbjct: 208 ITFEKIEEEFGATVRHIVEGETKVSKLGKLKCKTESETIQDVKADDLRQMFLAMTDEVRV 267

Query: 357 VLIKLADRLHNMMTLDALPLIKRQRFAKETMEIFVPLANRLGIYSWKEQLENLCFKHLNL 416
           +++KLADRLHNM TL  +P  K+   A ET+++F PLA  LG+YS K +LENL F +++ 
Sbjct: 268 IIVKLADRLHNMRTLCHMPPHKQSSIAGETLQVFAPLAKLLGMYSIKSELENLSFMYVSA 327

Query: 417 EQHKDLSSKLMGLY--DEAIIYSAIQKLERALEDKGISYHVVTGRH-----KSVYSVHCK 476
           E +  ++S++  LY   E  +  A + L + +ED      V          K  YS++  
Sbjct: 328 EDYDRVTSRIANLYKEHEKELTEANRILVKKIEDDQFLDLVTVNTDVRSVCKETYSIYKA 387

Query: 477 MLKKNLTMNEIHDI-HGLRLIVE------------KEEDCYEALRIVHQLWPEVPGKLKD 536
            LK   ++N+ + I   LR++V+             ++ CY  L +VH++W  +P  +KD
Sbjct: 388 ALKSKGSINDYNQIAQQLRIVVKPKPSVGVGPLCSPQQICYHVLGLVHEIWKPIPRTVKD 447

Query: 537 YISKPKLNGYFSHCSFYCFISYIFLLLYNLMVNRYQSIHTVV---RGEGDIPLEVQIRTK 588
           YI+ PK NG                         YQS+HT V     E    LEVQIRT+
Sbjct: 448 YIATPKPNG-------------------------YQSLHTTVIPFLYESMFRLEVQIRTE 499

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022152608.10.0e+0096.32probable GTP diphosphokinase RSH2, chloroplastic [Momordica charantia][more]
XP_038879703.10.0e+0088.70probable GTP diphosphokinase RSH2, chloroplastic [Benincasa hispida][more]
XP_022955553.10.0e+0088.17probable GTP diphosphokinase RSH3, chloroplastic [Cucurbita moschata] >XP_022955... [more]
XP_004148566.10.0e+0087.91probable GTP diphosphokinase RSH2, chloroplastic [Cucumis sativus] >XP_031745354... [more]
KAG7018260.10.0e+0087.91putative GTP diphosphokinase RSH3, chloroplastic, partial [Cucurbita argyrosperm... [more]
Match NameE-valueIdentityDescription
Q9LVJ33.0e-26764.83Probable GTP diphosphokinase RSH2, chloroplastic OS=Arabidopsis thaliana OX=3702... [more]
Q9SYH12.0e-26664.49Probable GTP diphosphokinase RSH3, chloroplastic OS=Arabidopsis thaliana OX=3702... [more]
Q9M5P59.1e-26464.23Probable GTP diphosphokinase RSH3, chloroplastic OS=Arabidopsis thaliana OX=3702... [more]
Q9M5P63.0e-25963.70Probable GTP diphosphokinase RSH2, chloroplastic OS=Arabidopsis thaliana OX=3702... [more]
Q67UU03.8e-23858.96Probable GTP diphosphokinase RSH3, chloroplastic OS=Oryza sativa subsp. japonica... [more]
Match NameE-valueIdentityDescription
A0A6J1DFB10.0e+0096.32GTP diphosphokinase OS=Momordica charantia OX=3673 GN=LOC111020291 PE=3 SV=1[more]
A0A6J1GVE80.0e+0088.17GTP diphosphokinase OS=Cucurbita moschata OX=3662 GN=LOC111457535 PE=3 SV=1[more]
A0A0A0L9B10.0e+0087.91GTP diphosphokinase OS=Cucumis sativus OX=3659 GN=Csa_3G651840 PE=3 SV=1[more]
A0A1S4E4I00.0e+0087.78GTP diphosphokinase OS=Cucumis melo OX=3656 GN=LOC103501986 PE=3 SV=1[more]
A0A5D3CA850.0e+0087.78GTP diphosphokinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold606G... [more]
Match NameE-valueIdentityDescription
AT3G14050.12.1e-26864.83RELA/SPOT homolog 2 [more]
AT1G54130.11.4e-26764.49RELA/SPOT homolog 3 [more]
AT4G02260.32.1e-5036.17RELA/SPOT homolog 1 [more]
AT4G02260.22.1e-5036.17RELA/SPOT homolog 1 [more]
AT4G02260.13.1e-4936.07RELA/SPOT homolog 1 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003607HD/PDEase domainSMARTSM00471hd_13coord: 257..374
e-value: 5.2E-7
score: 39.3
IPR003607HD/PDEase domainCDDcd00077HDccoord: 259..387
e-value: 1.74518E-6
score: 46.1785
IPR007685RelA/SpoTSMARTSM00954RelA_SpoT_2coord: 455..590
e-value: 1.6E-42
score: 157.2
IPR007685RelA/SpoTPFAMPF04607RelA_SpoTcoord: 544..589
e-value: 1.8E-11
score: 44.3
coord: 455..525
e-value: 2.8E-17
score: 63.1
IPR007685RelA/SpoTCDDcd05399NT_Rel-Spo_likecoord: 433..578
e-value: 2.96344E-36
score: 130.93
NoneNo IPR availablePFAMPF13328HD_4coord: 246..396
e-value: 1.5E-37
score: 128.8
NoneNo IPR availableGENE3D1.10.3210.10Hypothetical protein af1432coord: 216..412
e-value: 1.2E-57
score: 196.6
NoneNo IPR availablePANTHERPTHR21262GUANOSINE-3',5'-BIS DIPHOSPHATE 3'-PYROPHOSPHOHYDROLASEcoord: 1..525
NoneNo IPR availablePANTHERPTHR21262:SF35SUBFAMILY NOT NAMEDcoord: 1..525
NoneNo IPR availablePANTHERPTHR21262:SF35SUBFAMILY NOT NAMEDcoord: 545..751
NoneNo IPR availablePANTHERPTHR21262GUANOSINE-3',5'-BIS DIPHOSPHATE 3'-PYROPHOSPHOHYDROLASEcoord: 545..751
NoneNo IPR availablePROSITEPS50889S4coord: 704..740
score: 9.733032
NoneNo IPR availableSUPERFAMILY109604HD-domain/PDEase-likecoord: 221..412
IPR043519Nucleotidyltransferase superfamilyGENE3D3.30.460.10Beta Polymerase, domain 2coord: 425..570
e-value: 5.0E-36
score: 125.6
IPR043519Nucleotidyltransferase superfamilySUPERFAMILY81301Nucleotidyltransferasecoord: 402..622
IPR006674HD domainPROSITEPS51831HDcoord: 261..365
score: 15.718876
IPR002942RNA-binding S4 domainCDDcd00165S4coord: 704..739
e-value: 0.00613164
score: 34.1474

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS016191.1MS016191.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0015969 guanosine tetraphosphate metabolic process
molecular_function GO:0003723 RNA binding