MS016177 (gene) Bitter gourd (TR) v1

BLAST of MS016177 vs. NCBI nr
Match: XP_022152622.1 (RING-H2 finger protein ATL3-like [Momordica charantia])

HSP 1 Score: 559.3 bits (1440), Expect = 1.9e-155
Identity = 283/286 (98.95%), Postives = 285/286 (99.65%), Query Frame = 0

Query: 1   MEDEYKLSRHSGDSVTVELPAMVIVTAVLVFFLVLVFVLLLQLYARWLWSRIEDPTPLPV 60
           MEDEYKLSRHSGDSVTVELPAMVIVTAVLVFFLVLVFVLLLQLYARWLWSRIEDPTPLPV
Sbjct: 34  MEDEYKLSRHSGDSVTVELPAMVIVTAVLVFFLVLVFVLLLQLYARWLWSRIEDPTPLPV 93

Query: 61  EARRRRRRRRRFDFSSVQDPNSGRGLDPAVLRSLPVLIFHPENFKDGLECAVCLSEVVEG 120
           EARRRRRRRRRFDFSSVQDPNSGRGLDP VLRSLPVLIFHPENFKDGLECAVCLSEV+EG
Sbjct: 94  EARRRRRRRRRFDFSSVQDPNSGRGLDPTVLRSLPVLIFHPENFKDGLECAVCLSEVIEG 153

Query: 121 EKTKLLHDCCHGFHSDCIDMWFQSHSTCPLCRNPVTAAAAETSQVQEEDSASGSSSESPI 180
           EKTKLLHDCCHGFHSDCIDMWFQSHSTCPLCRNPVTAAAAETSQVQEEDSASGSSSESPI
Sbjct: 154 EKTKLLHDCCHGFHSDCIDMWFQSHSTCPLCRNPVTAAAAETSQVQEEDSASGSSSESPI 213

Query: 181 FPTNVLFWGNQAQVTSAGACPELEESSSQITSPAASSSASAAGHPDAELTIDVHSDLQFP 240
           FPTNVLFWGNQAQVTSAGACPELEESSSQITSPAASSSASAAGHPDAELTID+HSDLQFP
Sbjct: 214 FPTNVLFWGNQAQVTSAGACPELEESSSQITSPAASSSASAAGHPDAELTIDMHSDLQFP 273

Query: 241 PLAPNEERKLPVATRLRSLKRLLSRQRRVNPCNNTTSPNDEMEQLA 287
           PLAPNEERKLPVATRLRSLKRLLSRQRRVNPCNNTTSPNDEMEQLA
Sbjct: 274 PLAPNEERKLPVATRLRSLKRLLSRQRRVNPCNNTTSPNDEMEQLA 319

BLAST of MS016177 vs. NCBI nr
Match: XP_038895336.1 (RING-H2 finger protein ATL3-like [Benincasa hispida])

HSP 1 Score: 444.5 bits (1142), Expect = 6.9e-121
Identity = 231/285 (81.05%), Postives = 252/285 (88.42%), Query Frame = 0

Query: 1   MEDEYKLSRHSGDSVTVELPAMVIVTAVLVFFLVLVFVLLLQLYARWLWSRIEDPTPLPV 60
           M DEY++++HSGDSV +ELP MVIVTA+LVFFLVLVFVLLLQLYARWLWSRIEDPTPLP+
Sbjct: 1   MGDEYRMTQHSGDSVNIELPGMVIVTAILVFFLVLVFVLLLQLYARWLWSRIEDPTPLPL 60

Query: 61  EARRRRRRRRRFDFSSVQDPNSGRGLDPAVLRSLPVLIFHPENFKDGLECAVCLSEVVEG 120
           E   RRRRRRRF+FS+VQDPNSGRGLDP VLRSLPVLIFHP+NFKDGLECAVCLSE+VEG
Sbjct: 61  ET--RRRRRRRFEFSAVQDPNSGRGLDPTVLRSLPVLIFHPDNFKDGLECAVCLSELVEG 120

Query: 121 EKTKLLHDCCHGFHSDCIDMWFQSHSTCPLCRNPVTAAAAETSQVQEEDSASGSSSESPI 180
           EK KLLHDC HGFHSDCIDMWFQSHSTCPLCRNPVTAAAA+T ++Q EDS SG SSESP 
Sbjct: 121 EKAKLLHDCFHGFHSDCIDMWFQSHSTCPLCRNPVTAAAADTGELQGEDSTSGLSSESPT 180

Query: 181 FPTNVLFWGNQAQVTSAGACPELEESSSQITSPAASSSASAAGHPDAELTIDVHSDLQFP 240
           FPTNVL WGNQAQVTSAGAC E+EE SSQ   PAASSSASA+ + DAEL IDV SDLQF 
Sbjct: 181 FPTNVLIWGNQAQVTSAGACLEVEEGSSQNPFPAASSSASAS-YSDAELVIDVQSDLQFL 240

Query: 241 PLAPNEERKLPVATRLRSLKRLLSRQRRVNPCNNTTSPNDEMEQL 286
           P+A NE+ KLPV TRLRSLKRLLSRQRRVNPC N  S N E+EQ+
Sbjct: 241 PIATNEDGKLPVVTRLRSLKRLLSRQRRVNPC-NPNSANAEIEQV 281

BLAST of MS016177 vs. NCBI nr
Match: KAG7018248.1 (RING-H2 finger protein ATL3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 442.2 bits (1136), Expect = 3.4e-120
Identity = 229/286 (80.07%), Postives = 255/286 (89.16%), Query Frame = 0

Query: 1   MEDEYKLSRHSGDSVTVELPAMVIVTAVLVFFLVLVFVLLLQLYARWLWSRIEDPTPLPV 60
           MED+YKLS+HSGDSVT+ELP MVIVTA+LVF LVLVFVLLLQLYARWLWSRIEDP+PLPV
Sbjct: 1   MEDDYKLSQHSGDSVTIELPGMVIVTAILVFLLVLVFVLLLQLYARWLWSRIEDPSPLPV 60

Query: 61  EARRRRRRRRRFDFSSVQDPNSGRGLDPAVLRSLPVLIFHPENFKDGLECAVCLSEVVEG 120
           E   RRRRRRRF+FS++QDPNSGRGLDP +LRSLPVLIFHP++FKDGLECAVCLS++VEG
Sbjct: 61  ET--RRRRRRRFEFSAIQDPNSGRGLDPKILRSLPVLIFHPDDFKDGLECAVCLSDLVEG 120

Query: 121 EKTKLLHDCCHGFHSDCIDMWFQSHSTCPLCRNPVTAAAAETSQVQEEDSASGSSSESPI 180
           EK KLLHDC HGFHSDCIDMWF+SHSTCPLCRNPVT AAA++ ++Q+EDSASG SSESP 
Sbjct: 121 EKAKLLHDCFHGFHSDCIDMWFESHSTCPLCRNPVT-AAADSGELQDEDSASGLSSESPT 180

Query: 181 FPTNVLFWGNQAQVTSAGACPELEESSSQITSPAASSSASAAGHPDAELTIDVHSDLQFP 240
           FPTNVL WGNQ QVTSAGAC E+EE SSQ   PA SSSAS++ +P AEL IDV +DLQFP
Sbjct: 181 FPTNVLIWGNQEQVTSAGACLEVEEGSSQNPFPATSSSASSS-NPAAELVIDVQNDLQFP 240

Query: 241 PLAPNEERKLPVATRLRSLKRLLSRQRRVNPCNNTTSPNDEMEQLA 287
           PLAPNE+ KLPV TRLRSLKRLLSRQRRVNPC +  S N EMEQLA
Sbjct: 241 PLAPNEDGKLPVVTRLRSLKRLLSRQRRVNPC-DPNSANVEMEQLA 281

BLAST of MS016177 vs. NCBI nr
Match: XP_023527880.1 (RING-H2 finger protein ATL3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 441.8 bits (1135), Expect = 4.5e-120
Identity = 229/286 (80.07%), Postives = 255/286 (89.16%), Query Frame = 0

Query: 1   MEDEYKLSRHSGDSVTVELPAMVIVTAVLVFFLVLVFVLLLQLYARWLWSRIEDPTPLPV 60
           MED+YKLS+HSGDSVT+ELP MVIVTA+LVF LVLVFVLLLQLYARWLWSRIEDP+PLPV
Sbjct: 1   MEDDYKLSQHSGDSVTIELPGMVIVTAILVFLLVLVFVLLLQLYARWLWSRIEDPSPLPV 60

Query: 61  EARRRRRRRRRFDFSSVQDPNSGRGLDPAVLRSLPVLIFHPENFKDGLECAVCLSEVVEG 120
           E   RRRRRRRF+FS++ DPNSGRGLDP +LRSLPVLIFHP++FKDGLECAVCLS++VEG
Sbjct: 61  ET--RRRRRRRFEFSAIHDPNSGRGLDPKILRSLPVLIFHPDDFKDGLECAVCLSDLVEG 120

Query: 121 EKTKLLHDCCHGFHSDCIDMWFQSHSTCPLCRNPVTAAAAETSQVQEEDSASGSSSESPI 180
           EK KLLHDC HGFHSDCIDMWFQSHSTCPLCRNPVT AAA++ ++Q+EDSA+G SSESP 
Sbjct: 121 EKAKLLHDCFHGFHSDCIDMWFQSHSTCPLCRNPVT-AAADSGELQDEDSATGLSSESPT 180

Query: 181 FPTNVLFWGNQAQVTSAGACPELEESSSQITSPAASSSASAAGHPDAELTIDVHSDLQFP 240
           FPTNVL WGNQ QVTSAGAC E+EESSSQ   PA SSSAS++ +P AEL IDV +DLQFP
Sbjct: 181 FPTNVLIWGNQEQVTSAGACLEVEESSSQNPFPATSSSASSS-NPAAELVIDVQNDLQFP 240

Query: 241 PLAPNEERKLPVATRLRSLKRLLSRQRRVNPCNNTTSPNDEMEQLA 287
           PLAPNE+ KLPV TRLRSLKRLLSRQRRVNPC +  S N EMEQLA
Sbjct: 241 PLAPNEDGKLPVVTRLRSLKRLLSRQRRVNPC-DPNSANVEMEQLA 281

BLAST of MS016177 vs. NCBI nr
Match: KAG6581797.1 (RING-H2 finger protein ATL3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 440.7 bits (1132), Expect = 1.0e-119
Identity = 229/286 (80.07%), Postives = 254/286 (88.81%), Query Frame = 0

Query: 1   MEDEYKLSRHSGDSVTVELPAMVIVTAVLVFFLVLVFVLLLQLYARWLWSRIEDPTPLPV 60
           MED+YKLS+HSGDSVT+ELP MVIVTA+LVF LVLVFVLLLQLYARWLWSRIEDP+PLPV
Sbjct: 1   MEDDYKLSQHSGDSVTIELPGMVIVTAILVFLLVLVFVLLLQLYARWLWSRIEDPSPLPV 60

Query: 61  EARRRRRRRRRFDFSSVQDPNSGRGLDPAVLRSLPVLIFHPENFKDGLECAVCLSEVVEG 120
           E   RRRRRRRF+FS++QDPNSGRGLDP +LRSLPVLIFHP++FKDGLECAVCLS++VEG
Sbjct: 61  ET--RRRRRRRFEFSAIQDPNSGRGLDPKILRSLPVLIFHPDDFKDGLECAVCLSDLVEG 120

Query: 121 EKTKLLHDCCHGFHSDCIDMWFQSHSTCPLCRNPVTAAAAETSQVQEEDSASGSSSESPI 180
           EK KLLHDC HGFHSDCIDMWF+SHSTCPLCRNPVT AAA++  +Q+EDSASG SSESP 
Sbjct: 121 EKAKLLHDCFHGFHSDCIDMWFESHSTCPLCRNPVT-AAADSGGLQDEDSASGLSSESPT 180

Query: 181 FPTNVLFWGNQAQVTSAGACPELEESSSQITSPAASSSASAAGHPDAELTIDVHSDLQFP 240
           FPTNVL WGNQ QVTSAGAC E+EE SSQ   PA SSSAS++ +P AEL IDV +DLQFP
Sbjct: 181 FPTNVLIWGNQEQVTSAGACLEVEEGSSQNPFPATSSSASSS-NPAAELVIDVQNDLQFP 240

Query: 241 PLAPNEERKLPVATRLRSLKRLLSRQRRVNPCNNTTSPNDEMEQLA 287
           PLAPNE+ KLPV TRLRSLKRLLSRQRRVNPC +  S N EMEQLA
Sbjct: 241 PLAPNEDGKLPVVTRLRSLKRLLSRQRRVNPC-DPNSANVEMEQLA 281

BLAST of MS016177 vs. ExPASy Swiss-Prot
Match: Q9XF63 (RING-H2 finger protein ATL3 OS=Arabidopsis thaliana OX=3702 GN=ATL3 PE=2 SV=1)

HSP 1 Score: 180.6 bits (457), Expect = 2.4e-44
Identity = 120/317 (37.85%), Postives = 169/317 (53.31%), Query Frame = 0

Query: 8   SRHS---GDSVTVELPAMVIVTAVLVFFLVLVFVLLLQLYARWLWSRIEDPTPLPVEARR 67
           S HS   GD  T E+ + +I+TA++V F+ ++FVL+L LYA+  W RI+       + ++
Sbjct: 6   STHSSMFGDLSTEEVTSKIILTAIIVLFMAVLFVLILHLYAKLYWWRIDQLQQQQQQQQQ 65

Query: 68  R----------------RRRRRRFDFSSVQDPNSGRGLDPAVLRSLPVLIFHPENFKDGL 127
                            RR+RRRF F   QD  S  GL    L SLP++ F  ++ KDGL
Sbjct: 66  EQEQEEDQSSIAPPVVTRRQRRRFIFVPGQDALSNTGLTSFELSSLPIVFFRQDSCKDGL 125

Query: 128 ECAVCLSEVVEGEKTKLLHDCCHGFHSDCIDMWFQSHSTCPLCRN----PVTAAAAETSQ 187
           EC++CLSE+V+G+K +LL  C H FH +CIDMWFQSHSTCP+CRN    P  A++    Q
Sbjct: 126 ECSICLSELVKGDKARLLPKCNHSFHVECIDMWFQSHSTCPICRNTVLGPEQASSKRVEQ 185

Query: 188 VQE-----------EDSASGSSSESPIFPTNVLFWGNQAQVTSAGACPELEESSSQITSP 247
           V +            D+ S  S+ SP FPTNVL WG Q QV++       +E  +     
Sbjct: 186 VPDNAENAGTTNNNHDALSQLSTSSPEFPTNVLVWGRQDQVSTGNTNVGTQEDGAAGNGA 245

Query: 248 AASSSASAAGHPDAEL----TIDVHSDLQFPPLAPNEERKLPVATRLRSLKRLLSRQRRV 287
           + S  A      D+           S ++F  +   EE K P+ TRLRSL+R LSR +RV
Sbjct: 246 SQSQEAVVLDISDSSSRNHNVSSSSSSMRF--IVEEEEAKSPMTTRLRSLRRFLSRDKRV 305

BLAST of MS016177 vs. ExPASy Swiss-Prot
Match: P0C035 (RING-H2 finger protein ATL60 OS=Arabidopsis thaliana OX=3702 GN=ATL60 PE=2 SV=1)

HSP 1 Score: 159.5 bits (402), Expect = 5.8e-38
Identity = 113/289 (39.10%), Postives = 152/289 (52.60%), Query Frame = 0

Query: 18  ELPAMVIVTAVLVFFLVLVFVLLLQLYARWLWSRIEDPTPLPV--------EARRRRRRR 77
           E    V++ +++  F  ++F+LLL LYAR  W R+E    L +            R  RR
Sbjct: 19  ETMGKVLLFSIVSIFTGILFLLLLHLYARLFWWRVEQHFNLNLIQSDDPGSTVIGRNPRR 78

Query: 78  RRFDFS-SVQDPNSGRGLDPAVLRSLPVLIFHPENFKDGLECAVCLSEVVEGEKTKLLHD 137
           RRF F+ S +DP    GLD  +L+S+ V++F   +FKDGLECAVCLS++V+G+K ++L  
Sbjct: 79  RRFVFAQSQEDPLHNAGLDSKILQSIHVVVFKCTDFKDGLECAVCLSDLVDGDKARVLPR 138

Query: 138 CCHGFHSDCIDMWFQSHSTCPLCRNPV-----TAAAAETSQVQEEDSASGSS-------- 197
           C HGFH DCIDMWFQSHSTCPLCRN V     T         Q ++  SG S        
Sbjct: 139 CNHGFHVDCIDMWFQSHSTCPLCRNTVGSVEDTTHGGSEGLPQNQNFESGHSTNQHNPSQ 198

Query: 198 --------SESPI-FPTNVLFWGNQAQVTSAGACPELEESSSQITSPAASSSASAAGHPD 257
                   S  P+ FPTNVL WG+Q QV SAG     E  S    +        ++    
Sbjct: 199 DQSFVHEFSTEPLSFPTNVLVWGDQNQVRSAGLVVTEESPSGNFAASYNDHQQESSSTRS 258

Query: 258 AELT---IDV--HSDLQFPPLAPNEERKLPVATRLRSLKRLLSRQRRVN 271
            E+T   +D+  +S          EE K P+ TRLR LK +LSR++  N
Sbjct: 259 QEVTAVVVDIPDNSSENLSERIDEEEPKSPMFTRLRLLKNVLSREKTNN 307

BLAST of MS016177 vs. ExPASy Swiss-Prot
Match: Q8L9T5 (RING-H2 finger protein ATL2 OS=Arabidopsis thaliana OX=3702 GN=ATL2 PE=2 SV=2)

HSP 1 Score: 113.6 bits (283), Expect = 3.7e-24
Identity = 90/264 (34.09%), Postives = 128/264 (48.48%), Query Frame = 0

Query: 14  SVTVELPAMVIVTAVLVFFLVLVFVLLLQLYARWLWSRIEDPTPLPVEARRRRRRRRRFD 73
           S T  +   ++++A+++ F V++ ++ L LYARW   R           RRR R RR   
Sbjct: 21  SKTYAMSGKIMLSAIVILFFVVILMVFLHLYARWYLLRARRR-----HLRRRSRNRRATM 80

Query: 74  FSSVQDPN-------SGRGLDPAVLRSLPVLIFHPENFKDGLECAVCLSEVVEGEKTKLL 133
                DP+       + RGLDP V++SLPV  F  E  KD +ECAVCLSE  E E  ++L
Sbjct: 81  VFFTADPSTAATSVVASRGLDPNVIKSLPVFTFSDETHKDPIECAVCLSEFEESETGRVL 140

Query: 134 HDCCHGFHSDCIDMWFQSHSTCPLCRNPVTAAAA--ETSQVQEEDSASGSSSESPIFPTN 193
            +C H FH DCIDMWF SHSTCPLCR+ V + A    T+  +E +      S+    P  
Sbjct: 141 PNCQHTFHVDCIDMWFHSHSTCPLCRSLVESLAGIESTAAAREREVVIAVDSD----PVL 200

Query: 194 VLFWGNQAQVTSAGACPELEESSSQITSPAASSSASAAGHPDAELTIDVHSDLQFPPLAP 253
           V+        +S+G   E   S S       S    AA         +   +L       
Sbjct: 201 VI-----EPSSSSGLTDEPHGSGSSQMLREDSGRKPAAIEVPRRTFSEFEDELTRRDSPA 260

Query: 254 NEERKLPVATRLRSLKRLLSRQRR 269
           ++  + P+ +R+ S  R+LSR RR
Sbjct: 261 SQSFRSPM-SRMLSFTRMLSRDRR 269

BLAST of MS016177 vs. ExPASy Swiss-Prot
Match: Q9LUZ9 (RING-H2 finger protein ATL63 OS=Arabidopsis thaliana OX=3702 GN=ATL63 PE=2 SV=1)

HSP 1 Score: 103.2 bits (256), Expect = 4.9e-21
Identity = 62/167 (37.13%), Postives = 88/167 (52.69%), Query Frame = 0

Query: 23  VIVTAVLVFFLVLVFVLLLQLYARWLWSRIEDPTPLPVEARRRRRRRRR----------- 82
           V++ A++   LV++FVLLL  YAR+ WS            RRRRRR RR           
Sbjct: 29  VLLAALVFLLLVVLFVLLLHFYARFFWSPSHQDFSAAARHRRRRRRNRRRTVTTTRIIPS 88

Query: 83  -----FDFSSVQDP-----NSGRGLDPAVLRSLPVLIF--HPENFKDGLECAVCLSEVVE 142
                FD   V  P        +GLD +V+ S+P+ ++  + E   +  EC +CL     
Sbjct: 89  LPLGGFD-DGVSSPAATATRDDKGLDSSVISSIPLFVYEENEEEEDEEEECVICLGLWEA 148

Query: 143 GEKTKLLHDCCHGFHSDCIDMWFQSHSTCPLCRNPVTAAAAETSQVQ 167
           G+  + L +C HGFH +CIDMW  SHSTCPLCR+PV AA ++   ++
Sbjct: 149 GDFGRKLRNCGHGFHVECIDMWLSSHSTCPLCRSPVLAAVSDEENLK 194

BLAST of MS016177 vs. ExPASy Swiss-Prot
Match: Q9LRB7 (E3 ubiquitin-protein ligase EL5 OS=Oryza sativa subsp. japonica OX=39947 GN=EL5.1 PE=1 SV=1)

HSP 1 Score: 100.1 bits (248), Expect = 4.2e-20
Identity = 94/257 (36.58%), Postives = 115/257 (44.75%), Query Frame = 0

Query: 25  VTAVLVFF--LVLVFVLLLQLYARWLWSRIEDPTPLPVEARRRRRRRRRFDFSSVQDPNS 84
           V AVL+ F  L L FV LLQ Y      R    T      R RR R RR    S  D  +
Sbjct: 41  VAAVLIVFAALTLAFV-LLQCYCD---ERRRAVTTTSTSGRGRRPRPRRRS-GSGGDGGT 100

Query: 85  GRGLDPAVLRSLPVLIF-----------HPENFKDGLECAVCLSEVVEGEKTKLLHDCCH 144
           G G+DP VLRSLPV ++             E   DG+ECAVCL+E+ +GE+ + L  C H
Sbjct: 101 GGGVDPEVLRSLPVTVYSRSTAAAAAKEEEEEDDDGVECAVCLAELEDGEEARFLPRCGH 160

Query: 145 GFHSDCIDMWFQSHSTCPLCRNPVTAAAAETSQVQEEDSASGSSSESPIFPTNVLFWGNQ 204
           GFH++C+DMW  SHSTCPLCR  V         V  E  AS + S     P +VL   + 
Sbjct: 161 GFHAECVDMWLGSHSTCPLCRLTVVVPPPPLPPVPPEPPASYTVS----LPASVLLGLSD 220

Query: 205 AQVTSAGACPELEESSSQITSPAASSSASAAGHPDAELTIDVHSDLQFPPLAPNEERKLP 264
                AGA     E  S +      S+AS      A                    R  P
Sbjct: 221 ---HGAGAVTMTAEGRSTLVIEIPESAASTTPRDAA-------------------ARSSP 266

Query: 265 VATRLRSLKRLLSRQRR 269
              RLRSL+RL S  R+
Sbjct: 281 SLARLRSLRRLWSFGRQ 266

BLAST of MS016177 vs. ExPASy TrEMBL
Match: A0A6J1DGS1 (RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC111020300 PE=3 SV=1)

HSP 1 Score: 559.3 bits (1440), Expect = 9.3e-156
Identity = 283/286 (98.95%), Postives = 285/286 (99.65%), Query Frame = 0

Query: 1   MEDEYKLSRHSGDSVTVELPAMVIVTAVLVFFLVLVFVLLLQLYARWLWSRIEDPTPLPV 60
           MEDEYKLSRHSGDSVTVELPAMVIVTAVLVFFLVLVFVLLLQLYARWLWSRIEDPTPLPV
Sbjct: 34  MEDEYKLSRHSGDSVTVELPAMVIVTAVLVFFLVLVFVLLLQLYARWLWSRIEDPTPLPV 93

Query: 61  EARRRRRRRRRFDFSSVQDPNSGRGLDPAVLRSLPVLIFHPENFKDGLECAVCLSEVVEG 120
           EARRRRRRRRRFDFSSVQDPNSGRGLDP VLRSLPVLIFHPENFKDGLECAVCLSEV+EG
Sbjct: 94  EARRRRRRRRRFDFSSVQDPNSGRGLDPTVLRSLPVLIFHPENFKDGLECAVCLSEVIEG 153

Query: 121 EKTKLLHDCCHGFHSDCIDMWFQSHSTCPLCRNPVTAAAAETSQVQEEDSASGSSSESPI 180
           EKTKLLHDCCHGFHSDCIDMWFQSHSTCPLCRNPVTAAAAETSQVQEEDSASGSSSESPI
Sbjct: 154 EKTKLLHDCCHGFHSDCIDMWFQSHSTCPLCRNPVTAAAAETSQVQEEDSASGSSSESPI 213

Query: 181 FPTNVLFWGNQAQVTSAGACPELEESSSQITSPAASSSASAAGHPDAELTIDVHSDLQFP 240
           FPTNVLFWGNQAQVTSAGACPELEESSSQITSPAASSSASAAGHPDAELTID+HSDLQFP
Sbjct: 214 FPTNVLFWGNQAQVTSAGACPELEESSSQITSPAASSSASAAGHPDAELTIDMHSDLQFP 273

Query: 241 PLAPNEERKLPVATRLRSLKRLLSRQRRVNPCNNTTSPNDEMEQLA 287
           PLAPNEERKLPVATRLRSLKRLLSRQRRVNPCNNTTSPNDEMEQLA
Sbjct: 274 PLAPNEERKLPVATRLRSLKRLLSRQRRVNPCNNTTSPNDEMEQLA 319

BLAST of MS016177 vs. ExPASy TrEMBL
Match: A0A6J1IRZ5 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111479984 PE=3 SV=1)

HSP 1 Score: 439.5 bits (1129), Expect = 1.1e-119
Identity = 229/286 (80.07%), Postives = 253/286 (88.46%), Query Frame = 0

Query: 1   MEDEYKLSRHSGDSVTVELPAMVIVTAVLVFFLVLVFVLLLQLYARWLWSRIEDPTPLPV 60
           MED+YKLS+HSGDSV++ELP MVIVTA+LVF LVLVFVLLLQLYARWLWSRIEDPTPLPV
Sbjct: 1   MEDDYKLSQHSGDSVSIELPGMVIVTAILVFLLVLVFVLLLQLYARWLWSRIEDPTPLPV 60

Query: 61  EARRRRRRRRRFDFSSVQDPNSGRGLDPAVLRSLPVLIFHPENFKDGLECAVCLSEVVEG 120
           E   RRRRRRRF+FS+VQDPNSGRGLDP +LRSLPVLIFHP++FKDGLECAVCLS++VEG
Sbjct: 61  ET--RRRRRRRFEFSAVQDPNSGRGLDPKILRSLPVLIFHPDDFKDGLECAVCLSDLVEG 120

Query: 121 EKTKLLHDCCHGFHSDCIDMWFQSHSTCPLCRNPVTAAAAETSQVQEEDSASGSSSESPI 180
           EK KLLHDC HGFHSDCIDMWFQSHSTCPLCRNPVT AAA++ ++Q+EDSA+G SSESP 
Sbjct: 121 EKAKLLHDCFHGFHSDCIDMWFQSHSTCPLCRNPVT-AAADSGELQDEDSATGLSSESPT 180

Query: 181 FPTNVLFWGNQAQVTSAGACPELEESSSQITSPAASSSASAAGHPDAELTIDVHSDLQFP 240
           FPTNVL WGNQ QVTSAGAC E+EE SSQ   PA SSSAS++    AEL IDV +DLQFP
Sbjct: 181 FPTNVLIWGNQEQVTSAGACLEVEEGSSQNPFPATSSSASSSNQA-AELVIDVQNDLQFP 240

Query: 241 PLAPNEERKLPVATRLRSLKRLLSRQRRVNPCNNTTSPNDEMEQLA 287
           PLAPNE+ KLPV TRLRSLKRLLSRQRRVNPC +  S N EMEQLA
Sbjct: 241 PLAPNEDGKLPVVTRLRSLKRLLSRQRRVNPC-DPNSGNVEMEQLA 281

BLAST of MS016177 vs. ExPASy TrEMBL
Match: A0A6J1GV98 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111457742 PE=3 SV=1)

HSP 1 Score: 438.7 bits (1127), Expect = 1.8e-119
Identity = 227/286 (79.37%), Postives = 254/286 (88.81%), Query Frame = 0

Query: 1   MEDEYKLSRHSGDSVTVELPAMVIVTAVLVFFLVLVFVLLLQLYARWLWSRIEDPTPLPV 60
           MED+YKLS+HSGDSVT+ELP MVIVTA+LVF LVLVFVLLLQLYARWLWSRIEDP+PLPV
Sbjct: 1   MEDDYKLSQHSGDSVTIELPGMVIVTAILVFLLVLVFVLLLQLYARWLWSRIEDPSPLPV 60

Query: 61  EARRRRRRRRRFDFSSVQDPNSGRGLDPAVLRSLPVLIFHPENFKDGLECAVCLSEVVEG 120
           E   RRRRRRRF+FS++QDPNSGRGLDP +LRSLPVLIFHP++FKDGLECAVCLS++VEG
Sbjct: 61  ET--RRRRRRRFEFSAIQDPNSGRGLDPKILRSLPVLIFHPDDFKDGLECAVCLSDLVEG 120

Query: 121 EKTKLLHDCCHGFHSDCIDMWFQSHSTCPLCRNPVTAAAAETSQVQEEDSASGSSSESPI 180
           EK KLLHDC HGFHSDCIDMWF+SHSTCPLCRNPVT AAA++ ++Q+EDSASG SSESP 
Sbjct: 121 EKAKLLHDCFHGFHSDCIDMWFESHSTCPLCRNPVT-AAADSGELQDEDSASGLSSESPT 180

Query: 181 FPTNVLFWGNQAQVTSAGACPELEESSSQITSPAASSSASAAGHPDAELTIDVHSDLQFP 240
           FPTNVL WGNQ QVTSAGAC E++E SSQ   PAA SSAS++ +P AEL IDV +DLQFP
Sbjct: 181 FPTNVLIWGNQEQVTSAGACLEVKEGSSQNPFPAACSSASSS-NPAAELVIDVQNDLQFP 240

Query: 241 PLAPNEERKLPVATRLRSLKRLLSRQRRVNPCNNTTSPNDEMEQLA 287
           PL PNE+ KLPV TRLRSLKRLLSRQRRVNPC +  S N EMEQLA
Sbjct: 241 PLTPNEDGKLPVVTRLRSLKRLLSRQRRVNPC-DPNSANVEMEQLA 281

BLAST of MS016177 vs. ExPASy TrEMBL
Match: A0A5A7UJD5 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold522G001230 PE=3 SV=1)

HSP 1 Score: 433.3 bits (1113), Expect = 7.7e-118
Identity = 222/285 (77.89%), Postives = 247/285 (86.67%), Query Frame = 0

Query: 1   MEDEYKLSRHSGDSVTVELPAMVIVTAVLVFFLVLVFVLLLQLYARWLWSRIEDPTPLPV 60
           MEDEYKLS+HSGDSVT+ELP MVI+TA+LVFFLVLVFVLLLQLYA+WLWSRIEDPTPLP+
Sbjct: 1   MEDEYKLSQHSGDSVTIELPGMVIITAILVFFLVLVFVLLLQLYAKWLWSRIEDPTPLPL 60

Query: 61  EARRRRRRRRRFDFSSVQDPNSGRGLDPAVLRSLPVLIFHPENFKDGLECAVCLSEVVEG 120
           E   RRRRRRRF+FS+V DPNS RGLDP VLRSLPV+IFHP++FKDGLECAVCLS++VEG
Sbjct: 61  ET--RRRRRRRFEFSAVHDPNSARGLDPTVLRSLPVIIFHPDDFKDGLECAVCLSDLVEG 120

Query: 121 EKTKLLHDCCHGFHSDCIDMWFQSHSTCPLCRNPVTAAAAETSQVQEEDSASGSSSESPI 180
           EK KLLHDC HGFHSDCIDMWFQSHSTCPLCRNPVT A A+T+++ +EDS SG SSESP 
Sbjct: 121 EKAKLLHDCFHGFHSDCIDMWFQSHSTCPLCRNPVTTAVADTAELHDEDSTSGLSSESPT 180

Query: 181 FPTNVLFWGNQAQVTSAGACPELEESSSQITSPAASSSASAAGHPDAELTIDVHSDLQFP 240
           FPTNVL WGNQ QVTSAGA  E+EE  SQ   PAASSSAS   +PDAEL IDV +++QFP
Sbjct: 181 FPTNVLIWGNQGQVTSAGALLEVEEGPSQNHFPAASSSASTT-YPDAELVIDVQNEVQFP 240

Query: 241 PLAPNEERKLPVATRLRSLKRLLSRQRRVNPCNNTTSPNDEMEQL 286
           PL  NE+ KLPV TRLRSLKRLLSRQRRVNPC N+ S N E EQL
Sbjct: 241 PLTTNEDGKLPVVTRLRSLKRLLSRQRRVNPC-NSNSANAESEQL 281

BLAST of MS016177 vs. ExPASy TrEMBL
Match: A0A1S3CCE4 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103499208 PE=3 SV=1)

HSP 1 Score: 433.3 bits (1113), Expect = 7.7e-118
Identity = 222/285 (77.89%), Postives = 247/285 (86.67%), Query Frame = 0

Query: 1   MEDEYKLSRHSGDSVTVELPAMVIVTAVLVFFLVLVFVLLLQLYARWLWSRIEDPTPLPV 60
           MEDEYKLS+HSGDSVT+ELP MVI+TA+LVFFLVLVFVLLLQLYA+WLWSRIEDPTPLP+
Sbjct: 1   MEDEYKLSQHSGDSVTIELPGMVIITAILVFFLVLVFVLLLQLYAKWLWSRIEDPTPLPL 60

Query: 61  EARRRRRRRRRFDFSSVQDPNSGRGLDPAVLRSLPVLIFHPENFKDGLECAVCLSEVVEG 120
           E   RRRRRRRF+FS+V DPNS RGLDP VLRSLPV+IFHP++FKDGLECAVCLS++VEG
Sbjct: 61  ET--RRRRRRRFEFSAVHDPNSARGLDPTVLRSLPVIIFHPDDFKDGLECAVCLSDLVEG 120

Query: 121 EKTKLLHDCCHGFHSDCIDMWFQSHSTCPLCRNPVTAAAAETSQVQEEDSASGSSSESPI 180
           EK KLLHDC HGFHSDCIDMWFQSHSTCPLCRNPVT A A+T+++ +EDS SG SSESP 
Sbjct: 121 EKAKLLHDCFHGFHSDCIDMWFQSHSTCPLCRNPVTTAVADTAELHDEDSTSGLSSESPT 180

Query: 181 FPTNVLFWGNQAQVTSAGACPELEESSSQITSPAASSSASAAGHPDAELTIDVHSDLQFP 240
           FPTNVL WGNQ QVTSAGA  E+EE  SQ   PAASSSAS   +PDAEL IDV +++QFP
Sbjct: 181 FPTNVLIWGNQGQVTSAGALLEVEEGPSQNHFPAASSSASTT-YPDAELVIDVQNEVQFP 240

Query: 241 PLAPNEERKLPVATRLRSLKRLLSRQRRVNPCNNTTSPNDEMEQL 286
           PL  NE+ KLPV TRLRSLKRLLSRQRRVNPC N+ S N E EQL
Sbjct: 241 PLTTNEDGKLPVVTRLRSLKRLLSRQRRVNPC-NSNSANAESEQL 281

BLAST of MS016177 vs. TAIR 10
Match: AT1G72310.1 (RING/U-box superfamily protein )

HSP 1 Score: 180.6 bits (457), Expect = 1.7e-45
Identity = 120/317 (37.85%), Postives = 169/317 (53.31%), Query Frame = 0

Query: 8   SRHS---GDSVTVELPAMVIVTAVLVFFLVLVFVLLLQLYARWLWSRIEDPTPLPVEARR 67
           S HS   GD  T E+ + +I+TA++V F+ ++FVL+L LYA+  W RI+       + ++
Sbjct: 6   STHSSMFGDLSTEEVTSKIILTAIIVLFMAVLFVLILHLYAKLYWWRIDQLQQQQQQQQQ 65

Query: 68  R----------------RRRRRRFDFSSVQDPNSGRGLDPAVLRSLPVLIFHPENFKDGL 127
                            RR+RRRF F   QD  S  GL    L SLP++ F  ++ KDGL
Sbjct: 66  EQEQEEDQSSIAPPVVTRRQRRRFIFVPGQDALSNTGLTSFELSSLPIVFFRQDSCKDGL 125

Query: 128 ECAVCLSEVVEGEKTKLLHDCCHGFHSDCIDMWFQSHSTCPLCRN----PVTAAAAETSQ 187
           EC++CLSE+V+G+K +LL  C H FH +CIDMWFQSHSTCP+CRN    P  A++    Q
Sbjct: 126 ECSICLSELVKGDKARLLPKCNHSFHVECIDMWFQSHSTCPICRNTVLGPEQASSKRVEQ 185

Query: 188 VQE-----------EDSASGSSSESPIFPTNVLFWGNQAQVTSAGACPELEESSSQITSP 247
           V +            D+ S  S+ SP FPTNVL WG Q QV++       +E  +     
Sbjct: 186 VPDNAENAGTTNNNHDALSQLSTSSPEFPTNVLVWGRQDQVSTGNTNVGTQEDGAAGNGA 245

Query: 248 AASSSASAAGHPDAEL----TIDVHSDLQFPPLAPNEERKLPVATRLRSLKRLLSRQRRV 287
           + S  A      D+           S ++F  +   EE K P+ TRLRSL+R LSR +RV
Sbjct: 246 SQSQEAVVLDISDSSSRNHNVSSSSSSMRF--IVEEEEAKSPMTTRLRSLRRFLSRDKRV 305

BLAST of MS016177 vs. TAIR 10
Match: AT1G53820.1 (RING/U-box superfamily protein )

HSP 1 Score: 159.5 bits (402), Expect = 4.1e-39
Identity = 113/289 (39.10%), Postives = 152/289 (52.60%), Query Frame = 0

Query: 18  ELPAMVIVTAVLVFFLVLVFVLLLQLYARWLWSRIEDPTPLPV--------EARRRRRRR 77
           E    V++ +++  F  ++F+LLL LYAR  W R+E    L +            R  RR
Sbjct: 19  ETMGKVLLFSIVSIFTGILFLLLLHLYARLFWWRVEQHFNLNLIQSDDPGSTVIGRNPRR 78

Query: 78  RRFDFS-SVQDPNSGRGLDPAVLRSLPVLIFHPENFKDGLECAVCLSEVVEGEKTKLLHD 137
           RRF F+ S +DP    GLD  +L+S+ V++F   +FKDGLECAVCLS++V+G+K ++L  
Sbjct: 79  RRFVFAQSQEDPLHNAGLDSKILQSIHVVVFKCTDFKDGLECAVCLSDLVDGDKARVLPR 138

Query: 138 CCHGFHSDCIDMWFQSHSTCPLCRNPV-----TAAAAETSQVQEEDSASGSS-------- 197
           C HGFH DCIDMWFQSHSTCPLCRN V     T         Q ++  SG S        
Sbjct: 139 CNHGFHVDCIDMWFQSHSTCPLCRNTVGSVEDTTHGGSEGLPQNQNFESGHSTNQHNPSQ 198

Query: 198 --------SESPI-FPTNVLFWGNQAQVTSAGACPELEESSSQITSPAASSSASAAGHPD 257
                   S  P+ FPTNVL WG+Q QV SAG     E  S    +        ++    
Sbjct: 199 DQSFVHEFSTEPLSFPTNVLVWGDQNQVRSAGLVVTEESPSGNFAASYNDHQQESSSTRS 258

Query: 258 AELT---IDV--HSDLQFPPLAPNEERKLPVATRLRSLKRLLSRQRRVN 271
            E+T   +D+  +S          EE K P+ TRLR LK +LSR++  N
Sbjct: 259 QEVTAVVVDIPDNSSENLSERIDEEEPKSPMFTRLRLLKNVLSREKTNN 307

BLAST of MS016177 vs. TAIR 10
Match: AT3G16720.1 (TOXICOS EN LEVADURA 2 )

HSP 1 Score: 113.6 bits (283), Expect = 2.6e-25
Identity = 90/264 (34.09%), Postives = 128/264 (48.48%), Query Frame = 0

Query: 14  SVTVELPAMVIVTAVLVFFLVLVFVLLLQLYARWLWSRIEDPTPLPVEARRRRRRRRRFD 73
           S T  +   ++++A+++ F V++ ++ L LYARW   R           RRR R RR   
Sbjct: 21  SKTYAMSGKIMLSAIVILFFVVILMVFLHLYARWYLLRARRR-----HLRRRSRNRRATM 80

Query: 74  FSSVQDPN-------SGRGLDPAVLRSLPVLIFHPENFKDGLECAVCLSEVVEGEKTKLL 133
                DP+       + RGLDP V++SLPV  F  E  KD +ECAVCLSE  E E  ++L
Sbjct: 81  VFFTADPSTAATSVVASRGLDPNVIKSLPVFTFSDETHKDPIECAVCLSEFEESETGRVL 140

Query: 134 HDCCHGFHSDCIDMWFQSHSTCPLCRNPVTAAAA--ETSQVQEEDSASGSSSESPIFPTN 193
            +C H FH DCIDMWF SHSTCPLCR+ V + A    T+  +E +      S+    P  
Sbjct: 141 PNCQHTFHVDCIDMWFHSHSTCPLCRSLVESLAGIESTAAAREREVVIAVDSD----PVL 200

Query: 194 VLFWGNQAQVTSAGACPELEESSSQITSPAASSSASAAGHPDAELTIDVHSDLQFPPLAP 253
           V+        +S+G   E   S S       S    AA         +   +L       
Sbjct: 201 VI-----EPSSSSGLTDEPHGSGSSQMLREDSGRKPAAIEVPRRTFSEFEDELTRRDSPA 260

Query: 254 NEERKLPVATRLRSLKRLLSRQRR 269
           ++  + P+ +R+ S  R+LSR RR
Sbjct: 261 SQSFRSPM-SRMLSFTRMLSRDRR 269

BLAST of MS016177 vs. TAIR 10
Match: AT5G58580.1 (TOXICOS EN LEVADURA 63 )

HSP 1 Score: 103.2 bits (256), Expect = 3.5e-22
Identity = 62/167 (37.13%), Postives = 88/167 (52.69%), Query Frame = 0

Query: 23  VIVTAVLVFFLVLVFVLLLQLYARWLWSRIEDPTPLPVEARRRRRRRRR----------- 82
           V++ A++   LV++FVLLL  YAR+ WS            RRRRRR RR           
Sbjct: 29  VLLAALVFLLLVVLFVLLLHFYARFFWSPSHQDFSAAARHRRRRRRNRRRTVTTTRIIPS 88

Query: 83  -----FDFSSVQDP-----NSGRGLDPAVLRSLPVLIF--HPENFKDGLECAVCLSEVVE 142
                FD   V  P        +GLD +V+ S+P+ ++  + E   +  EC +CL     
Sbjct: 89  LPLGGFD-DGVSSPAATATRDDKGLDSSVISSIPLFVYEENEEEEDEEEECVICLGLWEA 148

Query: 143 GEKTKLLHDCCHGFHSDCIDMWFQSHSTCPLCRNPVTAAAAETSQVQ 167
           G+  + L +C HGFH +CIDMW  SHSTCPLCR+PV AA ++   ++
Sbjct: 149 GDFGRKLRNCGHGFHVECIDMWLSSHSTCPLCRSPVLAAVSDEENLK 194

BLAST of MS016177 vs. TAIR 10
Match: AT2G47560.1 (RING/U-box superfamily protein )

HSP 1 Score: 98.2 bits (243), Expect = 1.1e-20
Identity = 62/168 (36.90%), Postives = 90/168 (53.57%), Query Frame = 0

Query: 19  LPAMVIVTAVLVFFLVLVFVLLLQLYARWLWSRIEDPTPLPVEARRRRRRRRRFDFSSVQ 78
           L   +++++V+V F+ ++ +L    YARWL+ R            RR RRR R    ++ 
Sbjct: 24  LNGKIMLSSVIVLFVAVIMILCFHSYARWLFRR----------HNRRIRRRIRSHLRTLS 83

Query: 79  DPNSGRGLDPAVLRSLPVLIF---HPENFKDGLECAVCLSEVVEGEKTKLLHDCCHGFHS 138
                + LD AVL  +P+ ++   +P   ++  EC+VCLSE  E ++ +LL  C H FH 
Sbjct: 84  ASPRDQALDQAVLDKIPIFVYSSKNPPPPEEKEECSVCLSEFEEEDEGRLLPKCGHSFHV 143

Query: 139 DCIDMWFQSHSTCPLCRNPVTAAAAETSQVQEEDSASGSSSESPIFPT 184
           DCID WF+S STCPLCR PV          Q  ++ S SSS    FPT
Sbjct: 144 DCIDTWFRSRSTCPLCRAPV------QPPFQVIETGSSSSSSPLTFPT 175