MS016176 (gene) Bitter gourd (TR) v1

Overview


Name	MS016176
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Ubiquitin-conjugating enzyme E2 34
Location	scaffold9_2: 897212 .. 897771 (+)
RNA-Seq Expression	MS016176
Synteny	MS016176

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAACAAATCTGTTTCTCCTGATCTCGAGATGGATGATCCTGGTTTTGATGAATTTGATCCTCGCCTTAGCTTTTCCAAGGTGATTCACTCCCCTCTTAGATTACTCTGCCATTTTCATTTCTTAACGAAACGTGTTTGTAATTTTACCAGTTCTTGGAAGAAGCAAAACATCACGCCACAGTACAAGATTTCAAAGCCTCAAAGTCAACAGAAGAAGAAGCAGGAAGGAAATGGTTGGGGCAAGAGAAAAAGAGCAAGAAATCATGGAAAACCACCATCTTCTCGTGGTTGAAAAGTGACAAAAAGAGCAAGCCTCTGCCCAAACCAGAAATAAATCATCCTCACACATCCAGTAAAAGACTTGTTCATGTTTCTGGTCCGATTTACACCGGAGCTACGACCACCGACGGCAGACCCCGCCGCCGCCCGACGTCTGGACCGATCGCTAGTCTCTTCAACCCCAGTATGAGGACGGAGATGGAGATCCCTTATATGTGTCTCCACCAGCTTACCAGTCCCAATTCTAGTCATAATTATGGTCCAATTTATCTGGTTACA

mRNA sequence

ATGAACAAATCTGTTTCTCCTGATCTCGAGATGGATGATCCTGGTTTTGATGAATTTGATCCTCGCCTTAGCTTTTCCAAGTTCTTGGAAGAAGCAAAACATCACGCCACAGTACAAGATTTCAAAGCCTCAAAGTCAACAGAAGAAGAAGCAGGAAGGAAATGGTTGGGGCAAGAGAAAAAGAGCAAGAAATCATGGAAAACCACCATCTTCTCGTGGTTGAAAAGTGACAAAAAGAGCAAGCCTCTGCCCAAACCAGAAATAAATCATCCTCACACATCCAGTAAAAGACTTGTTCATGTTTCTGGTCCGATTTACACCGGAGCTACGACCACCGACGGCAGACCCCGCCGCCGCCCGACGTCTGGACCGATCGCTAGTCTCTTCAACCCCAGTATGAGGACGGAGATGGAGATCCCTTATATGTGTCTCCACCAGCTTACCAGTCCCAATTCTAGTCATAATTATGGTCCAATTTATCTGGTTACA

Coding sequence (CDS)

ATGAACAAATCTGTTTCTCCTGATCTCGAGATGGATGATCCTGGTTTTGATGAATTTGATCCTCGCCTTAGCTTTTCCAAGTTCTTGGAAGAAGCAAAACATCACGCCACAGTACAAGATTTCAAAGCCTCAAAGTCAACAGAAGAAGAAGCAGGAAGGAAATGGTTGGGGCAAGAGAAAAAGAGCAAGAAATCATGGAAAACCACCATCTTCTCGTGGTTGAAAAGTGACAAAAAGAGCAAGCCTCTGCCCAAACCAGAAATAAATCATCCTCACACATCCAGTAAAAGACTTGTTCATGTTTCTGGTCCGATTTACACCGGAGCTACGACCACCGACGGCAGACCCCGCCGCCGCCCGACGTCTGGACCGATCGCTAGTCTCTTCAACCCCAGTATGAGGACGGAGATGGAGATCCCTTATATGTGTCTCCACCAGCTTACCAGTCCCAATTCTAGTCATAATTATGGTCCAATTTATCTGGTTACA

Protein sequence

MNKSVSPDLEMDDPGFDEFDPRLSFSKFLEEAKHHATVQDFKASKSTEEEAGRKWLGQEKKSKKSWKTTIFSWLKSDKKSKPLPKPEINHPHTSSKRLVHVSGPIYTGATTTDGRPRRRPTSGPIASLFNPSMRTEMEIPYMCLHQLTSPNSSHNYGPIYLVT

Homology

[Close]

BLAST of MS016176 vs. NCBI nr
Match: XP_022152626.1 (uncharacterized protein LOC111020302 [Momordica charantia])

HSP 1 Score: 329.3 bits (843), Expect = 1.8e-86
Identity = 162/163 (99.39%), Postives = 162/163 (99.39%), Query Frame = 0


Query: 1   MNKSVSPDLEMDDPGFDEFDPRLSFSKFLEEAKHHATVQDFKASKSTEEEAGRKWLGQEK 60
           MNKSVSPDLEMDDPGFDEFDPRLSFSKFLEEAKHHATVQDFKASKSTEEEAGRKWLGQEK
Sbjct: 1   MNKSVSPDLEMDDPGFDEFDPRLSFSKFLEEAKHHATVQDFKASKSTEEEAGRKWLGQEK 60

Query: 61  KSKKSWKTTIFSWLKSDKKSKPLPKPEINHPHTSSKRLVHVSGPIYTGATTTDGRPRRRP 120
           KSKKSWKTTIFSWLKSDKKSKPLPKPEINHPHTSSKRLVHVSGPIYTGA TTDGRPRRRP
Sbjct: 61  KSKKSWKTTIFSWLKSDKKSKPLPKPEINHPHTSSKRLVHVSGPIYTGAMTTDGRPRRRP 120

Query: 121 TSGPIASLFNPSMRTEMEIPYMCLHQLTSPNSSHNYGPIYLVT 164
           TSGPIASLFNPSMRTEMEIPYMCLHQLTSPNSSHNYGPIYLVT
Sbjct: 121 TSGPIASLFNPSMRTEMEIPYMCLHQLTSPNSSHNYGPIYLVT 163

[Close]

BLAST of MS016176 vs. NCBI nr
Match: XP_038895377.1 (uncharacterized protein LOC120083617 [Benincasa hispida])

HSP 1 Score: 253.4 bits (646), Expect = 1.3e-63
Identity = 129/163 (79.14%), Postives = 140/163 (85.89%), Query Frame = 0


Query: 1   MNKSVSPDLEMDDPGFDEFDPRLSFSKFLEEAKHHATVQDFKASKSTEEEAGRKWLGQEK 60
           M KSVSPD EMDDPGFD+ D  L+FSKFL+EAK HA  QDFKA  STEEE GRKWL QEK
Sbjct: 33  MMKSVSPDYEMDDPGFDDIDLSLNFSKFLKEAKRHAIEQDFKA--STEEETGRKWLAQEK 92

Query: 61  KSKKSWKTTIFSWLKSDKKSKPLPKPEINHPHTSSKRLVHVSGPIYTGATTTDGRPRRRP 120
           KSKKSWK T+FSWLKS+KK+KP+PKPE N PHT +KR VHVSGPIYTGATTTD RPRR P
Sbjct: 93  KSKKSWKNTLFSWLKSEKKTKPMPKPEKN-PHTPNKRRVHVSGPIYTGATTTDSRPRRHP 152

Query: 121 TSGPIASLFNPSMRTEMEIPYMCLHQLTSPNSSHNYGPIYLVT 164
            SGPIASLFNPSMRT+MEIPYM LHQL +PNS+H YGPIYLVT
Sbjct: 153 MSGPIASLFNPSMRTDMEIPYMYLHQLNTPNSNHIYGPIYLVT 192

[Close]

BLAST of MS016176 vs. NCBI nr
Match: XP_011651731.1 (uncharacterized protein LOC101209455 [Cucumis sativus] >KGN58479.1 hypothetical protein Csa_002575 [Cucumis sativus])

HSP 1 Score: 241.1 bits (614), Expect = 6.6e-60
Identity = 123/163 (75.46%), Postives = 139/163 (85.28%), Query Frame = 0


Query: 1   MNKSVSPDLEMDDPGFDEFDPRLSFSKFLEEAKHHATVQDFKASKSTEEEAGRKWLGQEK 60
           M KS+S D EM++PGFD+FD  L+FSKFLEEAKHHAT Q FKA+ S EEE  RKWL QEK
Sbjct: 1   MKKSISLDYEMENPGFDDFDIGLNFSKFLEEAKHHATQQHFKAA-SIEEETERKWLAQEK 60

Query: 61  KSKKSWKTTIFSWLKSDKKSKPLPKPEINHPHTSSKRLVHVSGPIYTGATTTDGRPRRRP 120
           K+KKSWK T+FSWLKSDKKSK LPKPE N PHT +KR V+VSGPIY+ A T DGRPRRRP
Sbjct: 61  KNKKSWKNTLFSWLKSDKKSKSLPKPEKN-PHTPNKRRVYVSGPIYSEAMTIDGRPRRRP 120

Query: 121 TSGPIASLFNPSMRTEMEIPYMCLHQLTSPNSSHNYGPIYLVT 164
            SGPIASLFNP+MRTE EIPYMCLHQL++PN++ NYGPIYLVT
Sbjct: 121 MSGPIASLFNPTMRTETEIPYMCLHQLSAPNTNQNYGPIYLVT 161

[Close]

BLAST of MS016176 vs. NCBI nr
Match: KAG6606678.1 (hypothetical protein SDJN03_00020, partial [Cucurbita argyrosperma subsp. sororia] >KAG7036398.1 hypothetical protein SDJN02_00015 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 239.6 bits (610), Expect = 1.9e-59
Identity = 123/153 (80.39%), Postives = 130/153 (84.97%), Query Frame = 0


Query: 11  MDDPGFDEFDPRLSFSKFLEEAKHHATVQDFKASKSTEEEAGRKWLGQEKKSKKSWKTTI 70
           MDDP FD+FDPR +FSKFLEEAKHHA  +DFKA  ST+EE GRKWL Q KKSKKSWK T+
Sbjct: 1   MDDPCFDDFDPRTNFSKFLEEAKHHAKEEDFKA--STQEETGRKWLAQHKKSKKSWKNTL 60

Query: 71  FSWLKSDKKSKPLPKPEINHPHTSSKRLVHVSGPIYTGATTTDGRPRRRPTSGPIASLFN 130
           FSWL SDK SK LPK E N  HTS+KR VHVSGPIYTGATT DGRPR RPTSGPIASLFN
Sbjct: 61  FSWLNSDKTSKSLPKLERN-SHTSNKRRVHVSGPIYTGATTVDGRPRHRPTSGPIASLFN 120

Query: 131 PSMRTEMEIPYMCLHQLTSPNSSHNYGPIYLVT 164
           PSMRTEMEIPYMCLHQLTSP  +H YGPIYLVT
Sbjct: 121 PSMRTEMEIPYMCLHQLTSPIPNHYYGPIYLVT 150

[Close]

BLAST of MS016176 vs. NCBI nr
Match: XP_022949073.1 (uncharacterized protein LOC111452535 [Cucurbita moschata])

HSP 1 Score: 235.7 bits (600), Expect = 2.8e-58
Identity = 122/153 (79.74%), Postives = 129/153 (84.31%), Query Frame = 0


Query: 11  MDDPGFDEFDPRLSFSKFLEEAKHHATVQDFKASKSTEEEAGRKWLGQEKKSKKSWKTTI 70
           MDDP FD+FDPR +FSKFLEEAKHHA  +DFKA  ST+EE GRKWL Q KKSKKSWK T+
Sbjct: 1   MDDPCFDDFDPRTNFSKFLEEAKHHAKEEDFKA--STQEETGRKWLAQHKKSKKSWKNTL 60

Query: 71  FSWLKSDKKSKPLPKPEINHPHTSSKRLVHVSGPIYTGATTTDGRPRRRPTSGPIASLFN 130
           FSWL SDK SK LPK E N  HTS+KR VHVSG IYTGATT DGRPR RPTSGPIASLFN
Sbjct: 61  FSWLNSDKTSKSLPKLERN-SHTSNKRRVHVSGLIYTGATTVDGRPRHRPTSGPIASLFN 120

Query: 131 PSMRTEMEIPYMCLHQLTSPNSSHNYGPIYLVT 164
           PSMRTEMEIPYMCLHQLTSP  +H YGPIYLVT
Sbjct: 121 PSMRTEMEIPYMCLHQLTSPIPNHYYGPIYLVT 150

[Close]

BLAST of MS016176 vs. ExPASy TrEMBL
Match: A0A6J1DIA9 (uncharacterized protein LOC111020302 OS=Momordica charantia OX=3673 GN=LOC111020302 PE=4 SV=1)

HSP 1 Score: 329.3 bits (843), Expect = 8.9e-87
Identity = 162/163 (99.39%), Postives = 162/163 (99.39%), Query Frame = 0


Query: 1   MNKSVSPDLEMDDPGFDEFDPRLSFSKFLEEAKHHATVQDFKASKSTEEEAGRKWLGQEK 60
           MNKSVSPDLEMDDPGFDEFDPRLSFSKFLEEAKHHATVQDFKASKSTEEEAGRKWLGQEK
Sbjct: 1   MNKSVSPDLEMDDPGFDEFDPRLSFSKFLEEAKHHATVQDFKASKSTEEEAGRKWLGQEK 60

Query: 61  KSKKSWKTTIFSWLKSDKKSKPLPKPEINHPHTSSKRLVHVSGPIYTGATTTDGRPRRRP 120
           KSKKSWKTTIFSWLKSDKKSKPLPKPEINHPHTSSKRLVHVSGPIYTGA TTDGRPRRRP
Sbjct: 61  KSKKSWKTTIFSWLKSDKKSKPLPKPEINHPHTSSKRLVHVSGPIYTGAMTTDGRPRRRP 120

Query: 121 TSGPIASLFNPSMRTEMEIPYMCLHQLTSPNSSHNYGPIYLVT 164
           TSGPIASLFNPSMRTEMEIPYMCLHQLTSPNSSHNYGPIYLVT
Sbjct: 121 TSGPIASLFNPSMRTEMEIPYMCLHQLTSPNSSHNYGPIYLVT 163

[Close]

BLAST of MS016176 vs. ExPASy TrEMBL
Match: A0A0A0LC20 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G646660 PE=4 SV=1)

HSP 1 Score: 241.1 bits (614), Expect = 3.2e-60
Identity = 123/163 (75.46%), Postives = 139/163 (85.28%), Query Frame = 0


Query: 1   MNKSVSPDLEMDDPGFDEFDPRLSFSKFLEEAKHHATVQDFKASKSTEEEAGRKWLGQEK 60
           M KS+S D EM++PGFD+FD  L+FSKFLEEAKHHAT Q FKA+ S EEE  RKWL QEK
Sbjct: 1   MKKSISLDYEMENPGFDDFDIGLNFSKFLEEAKHHATQQHFKAA-SIEEETERKWLAQEK 60

Query: 61  KSKKSWKTTIFSWLKSDKKSKPLPKPEINHPHTSSKRLVHVSGPIYTGATTTDGRPRRRP 120
           K+KKSWK T+FSWLKSDKKSK LPKPE N PHT +KR V+VSGPIY+ A T DGRPRRRP
Sbjct: 61  KNKKSWKNTLFSWLKSDKKSKSLPKPEKN-PHTPNKRRVYVSGPIYSEAMTIDGRPRRRP 120

Query: 121 TSGPIASLFNPSMRTEMEIPYMCLHQLTSPNSSHNYGPIYLVT 164
            SGPIASLFNP+MRTE EIPYMCLHQL++PN++ NYGPIYLVT
Sbjct: 121 MSGPIASLFNPTMRTETEIPYMCLHQLSAPNTNQNYGPIYLVT 161

[Close]

BLAST of MS016176 vs. ExPASy TrEMBL
Match: A0A6J1GB15 (uncharacterized protein LOC111452535 OS=Cucurbita moschata OX=3662 GN=LOC111452535 PE=4 SV=1)

HSP 1 Score: 235.7 bits (600), Expect = 1.3e-58
Identity = 122/153 (79.74%), Postives = 129/153 (84.31%), Query Frame = 0


Query: 11  MDDPGFDEFDPRLSFSKFLEEAKHHATVQDFKASKSTEEEAGRKWLGQEKKSKKSWKTTI 70
           MDDP FD+FDPR +FSKFLEEAKHHA  +DFKA  ST+EE GRKWL Q KKSKKSWK T+
Sbjct: 1   MDDPCFDDFDPRTNFSKFLEEAKHHAKEEDFKA--STQEETGRKWLAQHKKSKKSWKNTL 60

Query: 71  FSWLKSDKKSKPLPKPEINHPHTSSKRLVHVSGPIYTGATTTDGRPRRRPTSGPIASLFN 130
           FSWL SDK SK LPK E N  HTS+KR VHVSG IYTGATT DGRPR RPTSGPIASLFN
Sbjct: 61  FSWLNSDKTSKSLPKLERN-SHTSNKRRVHVSGLIYTGATTVDGRPRHRPTSGPIASLFN 120

Query: 131 PSMRTEMEIPYMCLHQLTSPNSSHNYGPIYLVT 164
           PSMRTEMEIPYMCLHQLTSP  +H YGPIYLVT
Sbjct: 121 PSMRTEMEIPYMCLHQLTSPIPNHYYGPIYLVT 150

[Close]

BLAST of MS016176 vs. ExPASy TrEMBL
Match: A0A5D3D6L0 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold522G001220 PE=4 SV=1)

HSP 1 Score: 232.3 bits (591), Expect = 1.5e-57
Identity = 122/163 (74.85%), Postives = 137/163 (84.05%), Query Frame = 0


Query: 1   MNKSVSPDLEMDDPGFDEFDPRLSFSKFLEEAKHHATVQDFKASKSTEEEAGRKWLGQEK 60
           M KS S D EM++ GFD+FD  L+FS FLEEAKHHAT Q  KA+ S EEE GRK L QEK
Sbjct: 1   MKKSDSLDYEMENTGFDDFDIGLNFSMFLEEAKHHATRQHLKAA-SIEEETGRKRLAQEK 60

Query: 61  KSKKSWKTTIFSWLKSDKKSKPLPKPEINHPHTSSKRLVHVSGPIYTGATTTDGRPRRRP 120
           K+KKSWK T+FSWLKSDKKSK LPKPE N PHT +KR V+VSGP+Y+ ATTT+GRPRRRP
Sbjct: 61  KNKKSWKNTLFSWLKSDKKSKSLPKPEKN-PHTPNKRRVYVSGPMYSEATTTNGRPRRRP 120

Query: 121 TSGPIASLFNPSMRTEMEIPYMCLHQLTSPNSSHNYGPIYLVT 164
            SGPIASLFNPSMRTE EIPYMCL QL++PNS+HNYGPIYLVT
Sbjct: 121 MSGPIASLFNPSMRTETEIPYMCLQQLSAPNSNHNYGPIYLVT 161

[Close]

BLAST of MS016176 vs. ExPASy TrEMBL
Match: A0A1S3CDK1 (uncharacterized protein LOC103499209 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103499209 PE=4 SV=1)

HSP 1 Score: 231.9 bits (590), Expect = 1.9e-57
Identity = 122/163 (74.85%), Postives = 137/163 (84.05%), Query Frame = 0


Query: 1   MNKSVSPDLEMDDPGFDEFDPRLSFSKFLEEAKHHATVQDFKASKSTEEEAGRKWLGQEK 60
           M KS S D EM++ GFD+FD  L+FS FLEEAKHHAT Q  KA+ S EEE GRK L QEK
Sbjct: 1   MKKSDSLDYEMENTGFDDFDIGLNFSMFLEEAKHHATRQHLKAA-SIEEERGRKRLAQEK 60

Query: 61  KSKKSWKTTIFSWLKSDKKSKPLPKPEINHPHTSSKRLVHVSGPIYTGATTTDGRPRRRP 120
           K+KKSWK T+FSWLKSDKKSK LPKPE N PHT +KR V+VSGP+Y+ ATTT+GRPRRRP
Sbjct: 61  KNKKSWKNTLFSWLKSDKKSKSLPKPEKN-PHTPNKRRVYVSGPMYSEATTTNGRPRRRP 120

Query: 121 TSGPIASLFNPSMRTEMEIPYMCLHQLTSPNSSHNYGPIYLVT 164
            SGPIASLFNPSMRTE EIPYMCL QL++PNS+HNYGPIYLVT
Sbjct: 121 MSGPIASLFNPSMRTETEIPYMCLQQLSAPNSNHNYGPIYLVT 161

The following BLAST results are available for this feature:

BLAST of MS016176 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022152626.1	1.8e-86	99.39	uncharacterized protein LOC111020302 [Momordica charantia]	[more]
XP_038895377.1	1.3e-63	79.14	uncharacterized protein LOC120083617 [Benincasa hispida]	[more]
XP_011651731.1	6.6e-60	75.46	uncharacterized protein LOC101209455 [Cucumis sativus] >KGN58479.1 hypothetical ...	[more]
KAG6606678.1	1.9e-59	80.39	hypothetical protein SDJN03_00020, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_022949073.1	2.8e-58	79.74	uncharacterized protein LOC111452535 [Cucurbita moschata]	[more]

BLAST of MS016176 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of MS016176 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DIA9	8.9e-87	99.39	uncharacterized protein LOC111020302 OS=Momordica charantia OX=3673 GN=LOC111020...	[more]
A0A0A0LC20	3.2e-60	75.46	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G646660 PE=4 SV=1	[more]
A0A6J1GB15	1.3e-58	79.74	uncharacterized protein LOC111452535 OS=Cucurbita moschata OX=3662 GN=LOC1114525...	[more]
A0A5D3D6L0	1.5e-57	74.85	Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold...	[more]
A0A1S3CDK1	1.9e-57	74.85	uncharacterized protein LOC103499209 isoform X2 OS=Cucumis melo OX=3656 GN=LOC10...	[more]

BLAST of MS016176 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

Zoom :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 36..62
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 78..94
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 77..129
None	No IPR available	PANTHER	PTHR35488	OS05G0358900 PROTEIN-RELATED	coord: 1..163
None	No IPR available	PANTHER	PTHR35488:SF4	SUBFAMILY NOT NAMED	coord: 1..163

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS016176.1	MS016176.1	mRNA