MS016175 (gene) Bitter gourd (TR) v1

Overview


Name	MS016175
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	ubiquitin-conjugating enzyme E2 34-like
Location	scaffold9_2: 894348 .. 895714 (+)
RNA-Seq Expression	MS016175
Synteny	MS016175

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

AGCATGATCACCCCAAATGGTCGATTCATGGCACAGAAGAAAATTTGCTTATCTATGAGTGACTGTAAGTTTTTGGATTTTAAGGATGATCGTTCCTGCTTGTCTGTACTATAGAAATGATTCTAATTGACTTGATCTGTCTGTAGTTCACCCAGAAAGTTGGAATCCTATGTGGTCTGTGTCAAGGTGAGACATAATCCTTTTCTTTCTTTCTTTGCATACCCGAGTTCTCTTCTGCTAAAATTTTCTTTAAGTTATGAGTGGTTGCTTGGTATGTGACTAACTTTTCTCATTTTGCTTTTTCAACCTTCGCTTTTTAACAACAATTACAGCATACTAACCGGACTTCTTTCATTTATGGTGAGTACATTGTGGCCCTTGGCTCATTTTGTTTTTGGTTGTCTAATTGAAGATGGCTGCTTTTGTGCCTTTATCTGTTCTTATTATCTATTAATTTTGGGAGATGATCTAATTTCTTATGTAGATGGACAACAGTCCTACGACTGGCAGTGTGAATACTACTGCTGCTGAGAAGGAACGCTTGGCAAAGGCTTCTCTTGCATTCAATTGTAAAGAGTAAGTCCACCCACCAACTGTGCAGGATTTTGTGTCCTTAAAAAGTGGTTCCTCTTTTGCTTACCCCTTCTAAGGTTCCATCTCCCAGACACATTCCTCTGTTATGTAGGCTTGGTCAAGTTAATGAAGTAGCCCATAAAAATTATTGGAAATTTGTATGGCTTGCCTCTATCTTAAAAAAGAAAGGTCTTCTATTGGTTGTGACAGTTGGTTTTGGTTTGTTTTGGGGTTATTTTGACTAGTTTTTTGGGAAAACACGGACTCTAAAAACGTGAAACAATGATTATTCTACTTTAGGCTGTAAAACTTGGGCTTCCTCGAGTGTGTAAATTGCAAGTCATCTTTCTTGAATTTAGATCCCACACTCACTTTATTGGCATGTCATCCACAAACCACTGTCTGTTATTTTTCTTTTTCAACCCTCCATCGTCACTTTTACAATTGTAGTTGTACTTCAATGCTAATTTGTTGCGACAATTATTTATTTATTTTTTATTTTTTTTAATTCAAACAGCCCAAAATTTAGAAAACTGTTTCCGGAGTATGTGGAGAAATATAATCAGCAGCAGCAGCAGTCGGAGCAGCTTGTTGTGGAACATGATTCATCAAGGCCATCAGAGGAAGGCGAAGCTAGGCACCTGTTGGAGAAGGTCAAGCCAACGGAAGAGAATGTTAAGAAAGGTGATACTGAAGACGTTAAAAGAAATCGGAAGCAGTCGTTCCCTACATGGGTGATGTTGCTACTCTTCTCGATCTTCGGCGTGTTAATGGCACTGCCTCTCCTTCAACCG

mRNA sequence

AGCATGATCACCCCAAATGGTCGATTCATGGCACAGAAGAAAATTTGCTTATCTATGAGTGACTTTCACCCAGAAAGTTGGAATCCTATGTGGTCTGTGTCAAGCATACTAACCGGACTTCTTTCATTTATGATGGACAACAGTCCTACGACTGGCAGTGTGAATACTACTGCTGCTGAGAAGGAACGCTTGGCAAAGGCTTCTCTTGCATTCAATTGTAAAGACCCAAAATTTAGAAAACTGTTTCCGGAGTATGTGGAGAAATATAATCAGCAGCAGCAGCAGTCGGAGCAGCTTGTTGTGGAACATGATTCATCAAGGCCATCAGAGGAAGGCGAAGCTAGGCACCTGTTGGAGAAGGTCAAGCCAACGGAAGAGAATGTTAAGAAAGGTGATACTGAAGACGTTAAAAGAAATCGGAAGCAGTCGTTCCCTACATGGGTGATGTTGCTACTCTTCTCGATCTTCGGCGTGTTAATGGCACTGCCTCTCCTTCAACCG

Coding sequence (CDS)

AGCATGATCACCCCAAATGGTCGATTCATGGCACAGAAGAAAATTTGCTTATCTATGAGTGACTTTCACCCAGAAAGTTGGAATCCTATGTGGTCTGTGTCAAGCATACTAACCGGACTTCTTTCATTTATGATGGACAACAGTCCTACGACTGGCAGTGTGAATACTACTGCTGCTGAGAAGGAACGCTTGGCAAAGGCTTCTCTTGCATTCAATTGTAAAGACCCAAAATTTAGAAAACTGTTTCCGGAGTATGTGGAGAAATATAATCAGCAGCAGCAGCAGTCGGAGCAGCTTGTTGTGGAACATGATTCATCAAGGCCATCAGAGGAAGGCGAAGCTAGGCACCTGTTGGAGAAGGTCAAGCCAACGGAAGAGAATGTTAAGAAAGGTGATACTGAAGACGTTAAAAGAAATCGGAAGCAGTCGTTCCCTACATGGGTGATGTTGCTACTCTTCTCGATCTTCGGCGTGTTAATGGCACTGCCTCTCCTTCAACCG

Protein sequence

SMITPNGRFMAQKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDNSPTTGSVNTTAAEKERLAKASLAFNCKDPKFRKLFPEYVEKYNQQQQQSEQLVVEHDSSRPSEEGEARHLLEKVKPTEENVKKGDTEDVKRNRKQSFPTWVMLLLFSIFGVLMALPLLQP

Homology

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BLAST of MS016175 vs. NCBI nr
Match: XP_022152623.1 (ubiquitin-conjugating enzyme E2 34-like [Momordica charantia] >XP_022152624.1 ubiquitin-conjugating enzyme E2 34-like [Momordica charantia])

HSP 1 Score: 324.7 bits (831), Expect = 4.6e-85
Identity = 166/167 (99.40%), Postives = 166/167 (99.40%), Query Frame = 0


Query: 1   SMITPNGRFMAQKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDNSPTTGSVNTTAAE 60
           SMITPNGRFMAQKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDNSPTTGSVNTTAAE
Sbjct: 72  SMITPNGRFMAQKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDNSPTTGSVNTTAAE 131

Query: 61  KERLAKASLAFNCKDPKFRKLFPEYVEKYNQQQQQSEQLVVEHDSSRPSEEGEARHLLEK 120
           KERLAKASLAFNCKDPKFRKLFPEYVEKYNQQQQQSEQLVVEHDSSR SEEGEARHLLEK
Sbjct: 132 KERLAKASLAFNCKDPKFRKLFPEYVEKYNQQQQQSEQLVVEHDSSRLSEEGEARHLLEK 191

Query: 121 VKPTEENVKKGDTEDVKRNRKQSFPTWVMLLLFSIFGVLMALPLLQP 168
           VKPTEENVKKGDTEDVKRNRKQSFPTWVMLLLFSIFGVLMALPLLQP
Sbjct: 192 VKPTEENVKKGDTEDVKRNRKQSFPTWVMLLLFSIFGVLMALPLLQP 238

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BLAST of MS016175 vs. NCBI nr
Match: XP_038895346.1 (ubiquitin-conjugating enzyme E2 34-like [Benincasa hispida] >XP_038895351.1 ubiquitin-conjugating enzyme E2 34-like [Benincasa hispida] >XP_038895357.1 ubiquitin-conjugating enzyme E2 34-like [Benincasa hispida] >XP_038895365.1 ubiquitin-conjugating enzyme E2 34-like [Benincasa hispida])

HSP 1 Score: 282.0 bits (720), Expect = 3.5e-72
Identity = 144/167 (86.23%), Postives = 152/167 (91.02%), Query Frame = 0


Query: 1   SMITPNGRFMAQKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDNSPTTGSVNTTAAE 60
           SMITPNGRFM QKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDNSPTTGSVNTTAAE
Sbjct: 72  SMITPNGRFMTQKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDNSPTTGSVNTTAAE 131

Query: 61  KERLAKASLAFNCKDPKFRKLFPEYVEKYNQQQQQSEQLVVEHDSSRPSEEGEARHLLEK 120
           KERLAKASLAFNCK+P FRKLFPEYVEKYN QQQ+SEQLV E  S +  EEGE RH++EK
Sbjct: 132 KERLAKASLAFNCKNPTFRKLFPEYVEKYN-QQQKSEQLVAEQASPKSPEEGEGRHIMEK 191

Query: 121 VKPTEENVKKGDTEDVKRNRKQSFPTWVMLLLFSIFGVLMALPLLQP 168
           VK  EEN+ K D ED+KRNRKQSFPTW+MLLLFSIFGVLMALPLLQP
Sbjct: 192 VKSAEENINKADREDMKRNRKQSFPTWMMLLLFSIFGVLMALPLLQP 237

[Close]

BLAST of MS016175 vs. NCBI nr
Match: XP_004144451.1 (ubiquitin-conjugating enzyme E2 34 [Cucumis sativus] >XP_011651729.1 ubiquitin-conjugating enzyme E2 34 [Cucumis sativus] >KAE8650891.1 hypothetical protein Csa_001666 [Cucumis sativus])

HSP 1 Score: 279.6 bits (714), Expect = 1.7e-71
Identity = 143/167 (85.63%), Postives = 153/167 (91.62%), Query Frame = 0


Query: 1   SMITPNGRFMAQKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDNSPTTGSVNTTAAE 60
           SMITPNGRFM QKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDNSPTTGSVNTTAAE
Sbjct: 72  SMITPNGRFMTQKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDNSPTTGSVNTTAAE 131

Query: 61  KERLAKASLAFNCKDPKFRKLFPEYVEKYNQQQQQSEQLVVEHDSSRPSEEGEARHLLEK 120
           KERLAKASLAFNCK+P FRKLFPEYVEKYN QQQQSEQLV E  SS+ SEEGE +H++++
Sbjct: 132 KERLAKASLAFNCKNPTFRKLFPEYVEKYN-QQQQSEQLVAEQASSKSSEEGEGKHIMKR 191

Query: 121 VKPTEENVKKGDTEDVKRNRKQSFPTWVMLLLFSIFGVLMALPLLQP 168
           V+  EEN  K D ED+KRNRKQSFPTW+MLLLFSIFGVLMALPLLQP
Sbjct: 192 VRSMEENSNKADGEDMKRNRKQSFPTWLMLLLFSIFGVLMALPLLQP 237

[Close]

BLAST of MS016175 vs. NCBI nr
Match: XP_022955894.1 (ubiquitin-conjugating enzyme E2 34-like [Cucurbita moschata] >XP_022955896.1 ubiquitin-conjugating enzyme E2 34-like [Cucurbita moschata])

HSP 1 Score: 278.1 bits (710), Expect = 5.0e-71
Identity = 146/170 (85.88%), Postives = 155/170 (91.18%), Query Frame = 0


Query: 1   SMITPNGRFMAQKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDNSPTTGSVNTTAAE 60
           SMITPNGRFM QKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDNSPTTGSVNTTAAE
Sbjct: 72  SMITPNGRFMTQKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDNSPTTGSVNTTAAE 131

Query: 61  KERLAKASLAFNCKDPKFRKLFPEYVEKYNQQQQQSEQLVVEHDSSRPSEEGEARHLLEK 120
           KERLAKASLAFNCK+P FRKLFPEYVEKYN +Q QSEQLV E  SSRPSEEG+ARH++EK
Sbjct: 132 KERLAKASLAFNCKNPTFRKLFPEYVEKYN-EQHQSEQLVAEQASSRPSEEGKARHIMEK 191

Query: 121 VKP-TEENVKK--GDTEDVKRNRKQSFPTWVMLLLFSIFGVLMALPLLQP 168
           +K   EEN+ K   D ED+KRNRKQSFPTW+MLLLFSIFGVLMALPLLQP
Sbjct: 192 IKSREEENINKAAADREDMKRNRKQSFPTWMMLLLFSIFGVLMALPLLQP 240

[Close]

BLAST of MS016175 vs. NCBI nr
Match: XP_023527881.1 (ubiquitin-conjugating enzyme E2 34-like [Cucurbita pepo subsp. pepo] >XP_023527882.1 ubiquitin-conjugating enzyme E2 34-like [Cucurbita pepo subsp. pepo] >KAG6581795.1 Ubiquitin-conjugating enzyme E2 34, partial [Cucurbita argyrosperma subsp. sororia] >KAG7018246.1 Ubiquitin-conjugating enzyme E2 34 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 276.9 bits (707), Expect = 1.1e-70
Identity = 145/170 (85.29%), Postives = 155/170 (91.18%), Query Frame = 0


Query: 1   SMITPNGRFMAQKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDNSPTTGSVNTTAAE 60
           SMITPNGRFM QKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDNSPTTGSVNTTAAE
Sbjct: 72  SMITPNGRFMTQKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDNSPTTGSVNTTAAE 131

Query: 61  KERLAKASLAFNCKDPKFRKLFPEYVEKYNQQQQQSEQLVVEHDSSRPSEEGEARHLLEK 120
           KERLAKASLAFNCK+P FRKLFPEYVEKYN +Q QSEQLV E  SS+PSEEG+ARH++EK
Sbjct: 132 KERLAKASLAFNCKNPTFRKLFPEYVEKYN-EQHQSEQLVAEQASSKPSEEGKARHIMEK 191

Query: 121 VKP-TEENVKK--GDTEDVKRNRKQSFPTWVMLLLFSIFGVLMALPLLQP 168
           +K   EEN+ K   D ED+KRNRKQSFPTW+MLLLFSIFGVLMALPLLQP
Sbjct: 192 IKSREEENINKAAADREDMKRNRKQSFPTWMMLLLFSIFGVLMALPLLQP 240

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BLAST of MS016175 vs. ExPASy Swiss-Prot
Match: Q9SHI7 (Ubiquitin-conjugating enzyme E2 34 OS=Arabidopsis thaliana OX=3702 GN=UBC34 PE=2 SV=1)

HSP 1 Score: 208.8 bits (530), Expect = 4.9e-53
Identity = 108/166 (65.06%), Postives = 127/166 (76.51%), Query Frame = 0


Query: 1   SMITPNGRFMAQKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDNSPTTGSVNTTAAE 60
           +M TPNGRFM QKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMD SPTTGSVNTT  E
Sbjct: 72  TMTTPNGRFMTQKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDTSPTTGSVNTTVIE 131

Query: 61  KERLAKASLAFNCKDPKFRKLFPEYVEKYNQQQQQSEQLVVEHDSSRPSEEGEARHLLEK 120
           K+RLAK+SLAFNCK P FRKLFPEYVEKYN QQQ +EQ   +  +    ++ + +   EK
Sbjct: 132 KQRLAKSSLAFNCKTPAFRKLFPEYVEKYN-QQQLAEQATTQLTTPESPQKSDTKVESEK 191

Query: 121 VKPTEENVKKGDTEDVKRNRKQSFPTWVMLLLFSIFGVLMALPLLQ 167
                +   +G  ++ K+N KQ  P W++LLL S+FGV+MALPLLQ
Sbjct: 192 TIDPTKGDSEGGLKERKKNNKQGLPAWIILLLVSVFGVVMALPLLQ 236

[Close]

BLAST of MS016175 vs. ExPASy Swiss-Prot
Match: Q9FK29 (Probable ubiquitin-conjugating enzyme E2 33 OS=Arabidopsis thaliana OX=3702 GN=UBC33 PE=2 SV=1)

HSP 1 Score: 200.3 bits (508), Expect = 1.7e-50
Identity = 104/171 (60.82%), Postives = 131/171 (76.61%), Query Frame = 0


Query: 1   SMITPNGRFMAQKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDNSPTTGSVNTTAAE 60
           +M TPNGRF+ QKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDNSPTTGSVNT+ AE
Sbjct: 72  TMTTPNGRFVTQKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDNSPTTGSVNTSVAE 131

Query: 61  KERLAKASLAFNCKDPKFRKLFPEYVEKYNQQQQQSEQLVVEHDSSR-----PSEEGEAR 120
           K+RLAK+SLAFNCK   FRKLFPEYVEKY+QQQ   E+   +  ++      P ++    
Sbjct: 132 KQRLAKSSLAFNCKSVTFRKLFPEYVEKYSQQQVAEEEAATQQTTTSENQDFPQKDNAKV 191

Query: 121 HLLEKVKPTEENVKKGDTEDVKRNRKQSFPTWVMLLLFSIFGVLMALPLLQ 167
              + V   +E++++   ++ +RN+K++ P W++LLL SI GV+MALPLLQ
Sbjct: 192 ESEKSVGLKKESIQEVGLKERRRNKKEALPGWIVLLLVSIVGVVMALPLLQ 242

[Close]

BLAST of MS016175 vs. ExPASy Swiss-Prot
Match: Q8N2K1 (Ubiquitin-conjugating enzyme E2 J2 OS=Homo sapiens OX=9606 GN=UBE2J2 PE=1 SV=3)

HSP 1 Score: 119.4 bits (298), Expect = 3.9e-26
Identity = 60/107 (56.07%), Postives = 78/107 (72.90%), Query Frame = 0


Query: 2   MITPNGRFMAQKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDNSPTTGSVNTTAAEK 61
           MITPNGRF    ++CLS++DFHP++WNP WSVS+ILTGLLSFM++  PT GS+ T+   K
Sbjct: 80  MITPNGRFKCNTRLCLSITDFHPDTWNPAWSVSTILTGLLSFMVEKGPTLGSIETSDFTK 139

Query: 62  ERLAKASLAFNCKDPKFRKLFPEYVEKYNQQQQQSEQLVVEHDSSRP 109
            +LA  SLAFN KD  F +LFPE VE+  Q+Q+  ++L     SSRP
Sbjct: 140 RQLAVQSLAFNLKDKVFCELFPEVVEEIKQKQKAQDEL-----SSRP 181

[Close]

BLAST of MS016175 vs. ExPASy Swiss-Prot
Match: Q6P073 (Ubiquitin-conjugating enzyme E2 J2 OS=Mus musculus OX=10090 GN=Ube2j2 PE=1 SV=1)

HSP 1 Score: 117.5 bits (293), Expect = 1.5e-25
Identity = 58/107 (54.21%), Postives = 78/107 (72.90%), Query Frame = 0


Query: 2   MITPNGRFMAQKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDNSPTTGSVNTTAAEK 61
           MITPNGRF    ++CLS++DFHP++WNP WSVS+ILTGLLSFM++  PT GS+ T+   K
Sbjct: 80  MITPNGRFKCNTRLCLSITDFHPDTWNPAWSVSTILTGLLSFMVEKGPTLGSIETSDFTK 139

Query: 62  ERLAKASLAFNCKDPKFRKLFPEYVEKYNQQQQQSEQLVVEHDSSRP 109
           ++LA  SL FN KD  F +LFPE VE+  Q+Q+  ++L     S+RP
Sbjct: 140 KQLAAQSLVFNLKDKVFCELFPEVVEEIKQKQKAQDEL-----SNRP 181

[Close]

BLAST of MS016175 vs. ExPASy Swiss-Prot
Match: Q2TA03 (Ubiquitin-conjugating enzyme E2 J2 OS=Bos taurus OX=9913 GN=UBE2J2 PE=2 SV=1)

HSP 1 Score: 117.1 bits (292), Expect = 1.9e-25
Identity = 62/123 (50.41%), Postives = 81/123 (65.85%), Query Frame = 0


Query: 2   MITPNGRFMAQKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDNSPTTGSVNTTAAEK 61
           MITPNGRF    ++CLS++DFHP++WNP WSVS+ILTGLLSFM++  PT GS+ T+   K
Sbjct: 80  MITPNGRFKCNTRLCLSITDFHPDTWNPAWSVSTILTGLLSFMVEKGPTLGSIETSDFTK 139

Query: 62  ERLAKASLAFNCKDPKFRKLFPEYVEKYNQQQQQSEQLVVEHDSSRPS--------EEGE 117
            +LA  SL FN KD  F +LFPE VE+  Q+Q+  ++L     SSRP          +GE
Sbjct: 140 RQLAAQSLVFNLKDKVFCELFPEVVEEIKQKQKAQDEL-----SSRPQALPLPDVVPDGE 197

[Close]

BLAST of MS016175 vs. ExPASy TrEMBL
Match: A0A6J1DFC8 (ubiquitin-conjugating enzyme E2 34-like OS=Momordica charantia OX=3673 GN=LOC111020301 PE=4 SV=1)

HSP 1 Score: 324.7 bits (831), Expect = 2.2e-85
Identity = 166/167 (99.40%), Postives = 166/167 (99.40%), Query Frame = 0


Query: 1   SMITPNGRFMAQKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDNSPTTGSVNTTAAE 60
           SMITPNGRFMAQKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDNSPTTGSVNTTAAE
Sbjct: 72  SMITPNGRFMAQKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDNSPTTGSVNTTAAE 131

Query: 61  KERLAKASLAFNCKDPKFRKLFPEYVEKYNQQQQQSEQLVVEHDSSRPSEEGEARHLLEK 120
           KERLAKASLAFNCKDPKFRKLFPEYVEKYNQQQQQSEQLVVEHDSSR SEEGEARHLLEK
Sbjct: 132 KERLAKASLAFNCKDPKFRKLFPEYVEKYNQQQQQSEQLVVEHDSSRLSEEGEARHLLEK 191

Query: 121 VKPTEENVKKGDTEDVKRNRKQSFPTWVMLLLFSIFGVLMALPLLQP 168
           VKPTEENVKKGDTEDVKRNRKQSFPTWVMLLLFSIFGVLMALPLLQP
Sbjct: 192 VKPTEENVKKGDTEDVKRNRKQSFPTWVMLLLFSIFGVLMALPLLQP 238

[Close]

BLAST of MS016175 vs. ExPASy TrEMBL
Match: A0A6J1GV41 (ubiquitin-conjugating enzyme E2 34-like OS=Cucurbita moschata OX=3662 GN=LOC111457743 PE=4 SV=1)

HSP 1 Score: 278.1 bits (710), Expect = 2.4e-71
Identity = 146/170 (85.88%), Postives = 155/170 (91.18%), Query Frame = 0


Query: 1   SMITPNGRFMAQKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDNSPTTGSVNTTAAE 60
           SMITPNGRFM QKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDNSPTTGSVNTTAAE
Sbjct: 72  SMITPNGRFMTQKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDNSPTTGSVNTTAAE 131

Query: 61  KERLAKASLAFNCKDPKFRKLFPEYVEKYNQQQQQSEQLVVEHDSSRPSEEGEARHLLEK 120
           KERLAKASLAFNCK+P FRKLFPEYVEKYN +Q QSEQLV E  SSRPSEEG+ARH++EK
Sbjct: 132 KERLAKASLAFNCKNPTFRKLFPEYVEKYN-EQHQSEQLVAEQASSRPSEEGKARHIMEK 191

Query: 121 VKP-TEENVKK--GDTEDVKRNRKQSFPTWVMLLLFSIFGVLMALPLLQP 168
           +K   EEN+ K   D ED+KRNRKQSFPTW+MLLLFSIFGVLMALPLLQP
Sbjct: 192 IKSREEENINKAAADREDMKRNRKQSFPTWMMLLLFSIFGVLMALPLLQP 240

[Close]

BLAST of MS016175 vs. ExPASy TrEMBL
Match: A0A0A0L9H6 (UBC core domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G646650 PE=4 SV=1)

HSP 1 Score: 278.1 bits (710), Expect = 2.4e-71
Identity = 142/166 (85.54%), Postives = 152/166 (91.57%), Query Frame = 0


Query: 2   MITPNGRFMAQKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDNSPTTGSVNTTAAEK 61
           MITPNGRFM QKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDNSPTTGSVNTTAAEK
Sbjct: 236 MITPNGRFMTQKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDNSPTTGSVNTTAAEK 295

Query: 62  ERLAKASLAFNCKDPKFRKLFPEYVEKYNQQQQQSEQLVVEHDSSRPSEEGEARHLLEKV 121
           ERLAKASLAFNCK+P FRKLFPEYVEKYN QQQQSEQLV E  SS+ SEEGE +H++++V
Sbjct: 296 ERLAKASLAFNCKNPTFRKLFPEYVEKYN-QQQQSEQLVAEQASSKSSEEGEGKHIMKRV 355

Query: 122 KPTEENVKKGDTEDVKRNRKQSFPTWVMLLLFSIFGVLMALPLLQP 168
           +  EEN  K D ED+KRNRKQSFPTW+MLLLFSIFGVLMALPLLQP
Sbjct: 356 RSMEENSNKADGEDMKRNRKQSFPTWLMLLLFSIFGVLMALPLLQP 400

[Close]

BLAST of MS016175 vs. ExPASy TrEMBL
Match: A0A5D3D6L3 (Ubiquitin-conjugating enzyme E2 34-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold522G001210 PE=4 SV=1)

HSP 1 Score: 275.8 bits (704), Expect = 1.2e-70
Identity = 139/167 (83.23%), Postives = 152/167 (91.02%), Query Frame = 0


Query: 1   SMITPNGRFMAQKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDNSPTTGSVNTTAAE 60
           SMITPNGRFM QKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDNSPTTGSVNTTAAE
Sbjct: 72  SMITPNGRFMTQKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDNSPTTGSVNTTAAE 131

Query: 61  KERLAKASLAFNCKDPKFRKLFPEYVEKYNQQQQQSEQLVVEHDSSRPSEEGEARHLLEK 120
           KERLAK SLAFNCK+P FRKLFPEYVEKYN QQQQSEQLV E  S + SEEGE +H++++
Sbjct: 132 KERLAKTSLAFNCKNPTFRKLFPEYVEKYN-QQQQSEQLVAEQASPKSSEEGEGKHIMKR 191

Query: 121 VKPTEENVKKGDTEDVKRNRKQSFPTWVMLLLFSIFGVLMALPLLQP 168
           V+  EEN+ K D ED+K+NRKQSFPTW++LLLFSIFGVLMALPLLQP
Sbjct: 192 VRSMEENINKADGEDMKKNRKQSFPTWLLLLLFSIFGVLMALPLLQP 237

[Close]

BLAST of MS016175 vs. ExPASy TrEMBL
Match: A0A1S3CBV4 (ubiquitin-conjugating enzyme E2 34-like OS=Cucumis melo OX=3656 GN=LOC103499207 PE=4 SV=1)

HSP 1 Score: 275.0 bits (702), Expect = 2.0e-70
Identity = 138/167 (82.63%), Postives = 153/167 (91.62%), Query Frame = 0


Query: 1   SMITPNGRFMAQKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDNSPTTGSVNTTAAE 60
           SMITPNGRFM QKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDNSPTTGSVNTTAAE
Sbjct: 72  SMITPNGRFMTQKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDNSPTTGSVNTTAAE 131

Query: 61  KERLAKASLAFNCKDPKFRKLFPEYVEKYNQQQQQSEQLVVEHDSSRPSEEGEARHLLEK 120
           KERLAK+SLAFNCK+P FRKLFPEY+EKYN QQQQSEQLV E  S + SEEGE +H++++
Sbjct: 132 KERLAKSSLAFNCKNPTFRKLFPEYMEKYN-QQQQSEQLVAEQASPKSSEEGEGKHIMKR 191

Query: 121 VKPTEENVKKGDTEDVKRNRKQSFPTWVMLLLFSIFGVLMALPLLQP 168
           V+  EEN+ K D ED+K+NRKQSFPTW++LLLFSIFGVLMALPLLQP
Sbjct: 192 VRSMEENINKADGEDMKKNRKQSFPTWLLLLLFSIFGVLMALPLLQP 237

[Close]

BLAST of MS016175 vs. TAIR 10
Match: AT1G17280.1 (ubiquitin-conjugating enzyme 34 )

HSP 1 Score: 208.8 bits (530), Expect = 3.5e-54
Identity = 108/166 (65.06%), Postives = 127/166 (76.51%), Query Frame = 0


Query: 1   SMITPNGRFMAQKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDNSPTTGSVNTTAAE 60
           +M TPNGRFM QKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMD SPTTGSVNTT  E
Sbjct: 72  TMTTPNGRFMTQKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDTSPTTGSVNTTVIE 131

Query: 61  KERLAKASLAFNCKDPKFRKLFPEYVEKYNQQQQQSEQLVVEHDSSRPSEEGEARHLLEK 120
           K+RLAK+SLAFNCK P FRKLFPEYVEKYN QQQ +EQ   +  +    ++ + +   EK
Sbjct: 132 KQRLAKSSLAFNCKTPAFRKLFPEYVEKYN-QQQLAEQATTQLTTPESPQKSDTKVESEK 191

Query: 121 VKPTEENVKKGDTEDVKRNRKQSFPTWVMLLLFSIFGVLMALPLLQ 167
                +   +G  ++ K+N KQ  P W++LLL S+FGV+MALPLLQ
Sbjct: 192 TIDPTKGDSEGGLKERKKNNKQGLPAWIILLLVSVFGVVMALPLLQ 236

[Close]

BLAST of MS016175 vs. TAIR 10
Match: AT1G17280.2 (ubiquitin-conjugating enzyme 34 )

HSP 1 Score: 208.8 bits (530), Expect = 3.5e-54
Identity = 108/166 (65.06%), Postives = 127/166 (76.51%), Query Frame = 0


Query: 1   SMITPNGRFMAQKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDNSPTTGSVNTTAAE 60
           +M TPNGRFM QKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMD SPTTGSVNTT  E
Sbjct: 72  TMTTPNGRFMTQKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDTSPTTGSVNTTVIE 131

Query: 61  KERLAKASLAFNCKDPKFRKLFPEYVEKYNQQQQQSEQLVVEHDSSRPSEEGEARHLLEK 120
           K+RLAK+SLAFNCK P FRKLFPEYVEKYN QQQ +EQ   +  +    ++ + +   EK
Sbjct: 132 KQRLAKSSLAFNCKTPAFRKLFPEYVEKYN-QQQLAEQATTQLTTPESPQKSDTKVESEK 191

Query: 121 VKPTEENVKKGDTEDVKRNRKQSFPTWVMLLLFSIFGVLMALPLLQ 167
                +   +G  ++ K+N KQ  P W++LLL S+FGV+MALPLLQ
Sbjct: 192 TIDPTKGDSEGGLKERKKNNKQGLPAWIILLLVSVFGVVMALPLLQ 236

[Close]

BLAST of MS016175 vs. TAIR 10
Match: AT5G50430.1 (ubiquitin-conjugating enzyme 33 )

HSP 1 Score: 200.3 bits (508), Expect = 1.2e-51
Identity = 104/171 (60.82%), Postives = 131/171 (76.61%), Query Frame = 0


Query: 1   SMITPNGRFMAQKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDNSPTTGSVNTTAAE 60
           +M TPNGRF+ QKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDNSPTTGSVNT+ AE
Sbjct: 72  TMTTPNGRFVTQKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDNSPTTGSVNTSVAE 131

Query: 61  KERLAKASLAFNCKDPKFRKLFPEYVEKYNQQQQQSEQLVVEHDSSR-----PSEEGEAR 120
           K+RLAK+SLAFNCK   FRKLFPEYVEKY+QQQ   E+   +  ++      P ++    
Sbjct: 132 KQRLAKSSLAFNCKSVTFRKLFPEYVEKYSQQQVAEEEAATQQTTTSENQDFPQKDNAKV 191

Query: 121 HLLEKVKPTEENVKKGDTEDVKRNRKQSFPTWVMLLLFSIFGVLMALPLLQ 167
              + V   +E++++   ++ +RN+K++ P W++LLL SI GV+MALPLLQ
Sbjct: 192 ESEKSVGLKKESIQEVGLKERRRNKKEALPGWIVLLLVSIVGVVMALPLLQ 242

[Close]

BLAST of MS016175 vs. TAIR 10
Match: AT5G50430.2 (ubiquitin-conjugating enzyme 33 )

HSP 1 Score: 200.3 bits (508), Expect = 1.2e-51
Identity = 104/171 (60.82%), Postives = 131/171 (76.61%), Query Frame = 0


Query: 1   SMITPNGRFMAQKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDNSPTTGSVNTTAAE 60
           +M TPNGRF+ QKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDNSPTTGSVNT+ AE
Sbjct: 72  TMTTPNGRFVTQKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDNSPTTGSVNTSVAE 131

Query: 61  KERLAKASLAFNCKDPKFRKLFPEYVEKYNQQQQQSEQLVVEHDSSR-----PSEEGEAR 120
           K+RLAK+SLAFNCK   FRKLFPEYVEKY+QQQ   E+   +  ++      P ++    
Sbjct: 132 KQRLAKSSLAFNCKSVTFRKLFPEYVEKYSQQQVAEEEAATQQTTTSENQDFPQKDNAKV 191

Query: 121 HLLEKVKPTEENVKKGDTEDVKRNRKQSFPTWVMLLLFSIFGVLMALPLLQ 167
              + V   +E++++   ++ +RN+K++ P W++LLL SI GV+MALPLLQ
Sbjct: 192 ESEKSVGLKKESIQEVGLKERRRNKKEALPGWIVLLLVSIVGVVMALPLLQ 242

[Close]

BLAST of MS016175 vs. TAIR 10
Match: AT5G50430.3 (ubiquitin-conjugating enzyme 33 )

HSP 1 Score: 200.3 bits (508), Expect = 1.2e-51
Identity = 104/171 (60.82%), Postives = 131/171 (76.61%), Query Frame = 0


Query: 1   SMITPNGRFMAQKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDNSPTTGSVNTTAAE 60
           +M TPNGRF+ QKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDNSPTTGSVNT+ AE
Sbjct: 59  TMTTPNGRFVTQKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDNSPTTGSVNTSVAE 118

Query: 61  KERLAKASLAFNCKDPKFRKLFPEYVEKYNQQQQQSEQLVVEHDSSR-----PSEEGEAR 120
           K+RLAK+SLAFNCK   FRKLFPEYVEKY+QQQ   E+   +  ++      P ++    
Sbjct: 119 KQRLAKSSLAFNCKSVTFRKLFPEYVEKYSQQQVAEEEAATQQTTTSENQDFPQKDNAKV 178

Query: 121 HLLEKVKPTEENVKKGDTEDVKRNRKQSFPTWVMLLLFSIFGVLMALPLLQ 167
              + V   +E++++   ++ +RN+K++ P W++LLL SI GV+MALPLLQ
Sbjct: 179 ESEKSVGLKKESIQEVGLKERRRNKKEALPGWIVLLLVSIVGVVMALPLLQ 229

The following BLAST results are available for this feature:

BLAST of MS016175 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022152623.1	4.6e-85	99.40	ubiquitin-conjugating enzyme E2 34-like [Momordica charantia] >XP_022152624.1 ub...	[more]
XP_038895346.1	3.5e-72	86.23	ubiquitin-conjugating enzyme E2 34-like [Benincasa hispida] >XP_038895351.1 ubiq...	[more]
XP_004144451.1	1.7e-71	85.63	ubiquitin-conjugating enzyme E2 34 [Cucumis sativus] >XP_011651729.1 ubiquitin-c...	[more]
XP_022955894.1	5.0e-71	85.88	ubiquitin-conjugating enzyme E2 34-like [Cucurbita moschata] >XP_022955896.1 ubi...	[more]
XP_023527881.1	1.1e-70	85.29	ubiquitin-conjugating enzyme E2 34-like [Cucurbita pepo subsp. pepo] >XP_0235278...	[more]

BLAST of MS016175 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SHI7	4.9e-53	65.06	Ubiquitin-conjugating enzyme E2 34 OS=Arabidopsis thaliana OX=3702 GN=UBC34 PE=2...	[more]
Q9FK29	1.7e-50	60.82	Probable ubiquitin-conjugating enzyme E2 33 OS=Arabidopsis thaliana OX=3702 GN=U...	[more]
Q8N2K1	3.9e-26	56.07	Ubiquitin-conjugating enzyme E2 J2 OS=Homo sapiens OX=9606 GN=UBE2J2 PE=1 SV=3	[more]
Q6P073	1.5e-25	54.21	Ubiquitin-conjugating enzyme E2 J2 OS=Mus musculus OX=10090 GN=Ube2j2 PE=1 SV=1	[more]
Q2TA03	1.9e-25	50.41	Ubiquitin-conjugating enzyme E2 J2 OS=Bos taurus OX=9913 GN=UBE2J2 PE=2 SV=1	[more]

BLAST of MS016175 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DFC8	2.2e-85	99.40	ubiquitin-conjugating enzyme E2 34-like OS=Momordica charantia OX=3673 GN=LOC111...	[more]
A0A6J1GV41	2.4e-71	85.88	ubiquitin-conjugating enzyme E2 34-like OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]
A0A0A0L9H6	2.4e-71	85.54	UBC core domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G646650 PE...	[more]
A0A5D3D6L3	1.2e-70	83.23	Ubiquitin-conjugating enzyme E2 34-like OS=Cucumis melo var. makuwa OX=1194695 G...	[more]
A0A1S3CBV4	2.0e-70	82.63	ubiquitin-conjugating enzyme E2 34-like OS=Cucumis melo OX=3656 GN=LOC103499207 ...	[more]

BLAST of MS016175 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G17280.1	3.5e-54	65.06	ubiquitin-conjugating enzyme 34	[more]
AT1G17280.2	3.5e-54	65.06	ubiquitin-conjugating enzyme 34	[more]
AT5G50430.1	1.2e-51	60.82	ubiquitin-conjugating enzyme 33	[more]
AT5G50430.2	1.2e-51	60.82	ubiquitin-conjugating enzyme 33	[more]
AT5G50430.3	1.2e-51	60.82	ubiquitin-conjugating enzyme 33	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR016135	Ubiquitin-conjugating enzyme/RWD-like	GENE3D	3.10.110.10	Ubiquitin Conjugating Enzyme	coord: 1..107 e-value: 3.0E-29 score: 103.7
IPR016135	Ubiquitin-conjugating enzyme/RWD-like	SUPERFAMILY	54495	UBC-like	coord: 2..85
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 93..137
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 103..137
None	No IPR available	PANTHER	PTHR24068:SF135	UBIQUITIN-CONJUGATING ENZYME E2 J2	coord: 1..166
None	No IPR available	PANTHER	PTHR24068	UBIQUITIN-CONJUGATING ENZYME E2	coord: 1..166
IPR000608	Ubiquitin-conjugating enzyme E2	PROSITE	PS50127	UBC_2	coord: 1..91 score: 20.622341

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS016175.1	MS016175.1	mRNA