MS016170 (gene) Bitter gourd (TR) v1

Overview
NameMS016170
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
Descriptionautophagy-related protein 13b
Locationscaffold9_2: 856752 .. 857163 (+)
RNA-Seq ExpressionMS016170
SyntenyMS016170
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTTCTTCTCACAATACTCATTCCGAGACTGCAAAAACCGACCAAATTCTAACTGCTAAAACGCTACAAATTGTACTGGAATCGAGGTCCCCTTGTATCTCGTCGCGTAATTTCAGTGGGGAACAAGTTTTGTCGTCTCCATCTTCCTCCTGTTCGTGAATGCTCTGCAGCGTTAGAGAACATTGATCTCTGGCGTCAGAGCTACCACGAAACCATGGTGGTAGATGTTATTTTGGTTCAGACTCAGTGAATGCTTCTCCTAGAAAAGATCTTGTTAGGAACTTGTCTCTGAAAGAGAAATATTCACTCTTTCAATTGTGATAAGGACGAGTTTGGATGTGATACCAAGAGCGAGAAGATTGTAGAAAGATGGATGGTGCATTATGAAACTATAAAAAACAGGGATAGC

mRNA sequence

ATGGCTTCTTCTCACAATACTCATTCCGAGACTGCAAAAACCGACCAAATTCTAACTGCTAAAACGCTACAAATTGTACTGGAATCGAGGTCCCCTTGTATCTCGTCGCGTAATTTCAGTGGGGAACAAGTTTTGTCGTCTCCATCTTCCTCCGTTCGTGAATGCTCTGCAGCGTTAGAGAACATTGATCTCTGGCGTCAGAGCTACCACGAAACCATGGTGGTAGATGTTATTTTGGTTGACTCAGTGAATGCTTCTCCTAGAAAAGATCTTGTTAGGAACTTGTCTCTGAAAGAGAAATATTCACTCTTCAATTGTGATAAGGACGAGTTTGGATGTGATACCAAGAGCGAGAAGATTGTAGAAAGATGGATGGTGCATTATGAAACTATAAAAAACAGGGATAGC

Coding sequence (CDS)

ATGGCTTCTTCTCACAATACTCATTCCGAGACTGCAAAAACCGACCAAATTCTAACTGCTAAAACGCTACAAATTGTACTGGAATCGAGGTCCCCTTGTATCTCGTCGCGTAATTTCAGTGGGGAACAAGTTTTGTCGTCTCCATCTTCCTCCGTTCGTGAATGCTCTGCAGCGTTAGAGAACATTGATCTCTGGCGTCAGAGCTACCACGAAACCATGGTGGTAGATGTTATTTTGGTTGACTCAGTGAATGCTTCTCCTAGAAAAGATCTTGTTAGGAACTTGTCTCTGAAAGAGAAATATTCACTCTTCAATTGTGATAAGGACGAGTTTGGATGTGATACCAAGAGCGAGAAGATTGTAGAAAGATGGATGGTGCATTATGAAACTATAAAAAACAGGGATAGC

Protein sequence

MASSHNTHSETAKTDQILTAKTLQIVLESRSPCISSRNFSGEQVLSSPSSSVRECSAALENIDLWRQSYHETMVVDVILVDSVNASPRKDLVRNLSLKEKYSLFNCDKDEFGCDTKSEKIVERWMVHYETIKNRDS
Homology
BLAST of MS016170 vs. NCBI nr
Match: XP_022132934.1 (autophagy-related protein 13b [Momordica charantia] >XP_022132935.1 autophagy-related protein 13b [Momordica charantia])

HSP 1 Score: 227.6 bits (579), Expect = 6.3e-56
Identity = 128/162 (79.01%), Postives = 132/162 (81.48%), Query Frame = 0

Query: 1   MASSHNTHSETAKTDQILT---AKTLQIVLESRSPCISSRNFSGEQVLSSPSSS------ 60
           MASSHNTHSETAKT+QILT   AKTLQIVLESRSPCISSRNFSGEQVLSSPSSS      
Sbjct: 1   MASSHNTHSETAKTEQILTEFFAKTLQIVLESRSPCISSRNFSGEQVLSSPSSSSSSSSS 60

Query: 61  -----------VRECSAALENIDLWRQSYHETMVVDVILV------DSVNASPRKDLVRN 120
                      +RECSAALENIDLWRQSYHE MVVDVILV      DSVNASPRKDLVRN
Sbjct: 61  MRPRDKWFNLALRECSAALENIDLWRQSYHEPMVVDVILVQRQFGLDSVNASPRKDLVRN 120

Query: 121 LSLKEKYSL-FNCDKDEFGCDTKSEKIVERWMVHYETIKNRD 136
           LSLK+KYSL FN DKDEFGCDTKSEK+VERWMVHYET KNRD
Sbjct: 121 LSLKDKYSLSFNSDKDEFGCDTKSEKVVERWMVHYETRKNRD 162

BLAST of MS016170 vs. NCBI nr
Match: XP_038882060.1 (autophagy-related protein 13b [Benincasa hispida])

HSP 1 Score: 212.6 bits (540), Expect = 2.1e-51
Identity = 119/163 (73.01%), Postives = 126/163 (77.30%), Query Frame = 0

Query: 1   MASSHNTHSETAKTDQILT---AKTLQIVLESRSPCISSRNFSGEQVLSSPSSS------ 60
           MASSHNTHSETAK +QILT    KTLQI+LESR+PCISSRNFSGEQVLSSPSSS      
Sbjct: 1   MASSHNTHSETAKVEQILTEFFPKTLQIILESRAPCISSRNFSGEQVLSSPSSSSSSSSS 60

Query: 61  -----------VRECSAALENIDLWRQSYHETMVVDVILV------DSVNASPRKDLVRN 120
                      +RECSA LENIDLWR SYHE MVVDVILV      DSVNASPRKDLVRN
Sbjct: 61  MRPRDKWFNLALRECSATLENIDLWRPSYHEPMVVDVILVQRQFGLDSVNASPRKDLVRN 120

Query: 121 LSLKEKYSL-FNCDKDEFGCDTKSEKIVERWMVHYETIKNRDS 137
            SLKEKY L FN DKDEFGC TKSEK++ERWMVHYE+ KNRDS
Sbjct: 121 SSLKEKYPLSFNSDKDEFGCQTKSEKVIERWMVHYESRKNRDS 163

BLAST of MS016170 vs. NCBI nr
Match: XP_008441018.1 (PREDICTED: autophagy-related protein 13b isoform X2 [Cucumis melo])

HSP 1 Score: 208.4 bits (529), Expect = 4.0e-50
Identity = 118/163 (72.39%), Postives = 126/163 (77.30%), Query Frame = 0

Query: 1   MASSHNTHSETAKTDQILT---AKTLQIVLESRSPCISSRNFSGEQVLSSPSSS------ 60
           MASSHNTHSETAK +QILT    KTLQI+LESR+PCISSRNFSGEQVLSSPSSS      
Sbjct: 1   MASSHNTHSETAKVEQILTEFFPKTLQIILESRAPCISSRNFSGEQVLSSPSSSSSSSSS 60

Query: 61  -----------VRECSAALENIDLWRQSYHETMVVDVILV------DSVNASPRKDLVRN 120
                      +RECSA LENIDLWR +YHE MVVDVILV      DSVNASPRKDLVR+
Sbjct: 61  MRPRDKWFNLALRECSATLENIDLWRPNYHEPMVVDVILVQRQFGLDSVNASPRKDLVRH 120

Query: 121 LSLKEKYSL-FNCDKDEFGCDTKSEKIVERWMVHYETIKNRDS 137
           LSLKEKY L FN DKDEFG  TKSEK+VERWMVHYE+ KNRDS
Sbjct: 121 LSLKEKYPLSFNSDKDEFGSQTKSEKVVERWMVHYESRKNRDS 163

BLAST of MS016170 vs. NCBI nr
Match: XP_008441017.1 (PREDICTED: autophagy-related protein 13b isoform X1 [Cucumis melo])

HSP 1 Score: 208.4 bits (529), Expect = 4.0e-50
Identity = 118/163 (72.39%), Postives = 126/163 (77.30%), Query Frame = 0

Query: 1   MASSHNTHSETAKTDQILT---AKTLQIVLESRSPCISSRNFSGEQVLSSPSSS------ 60
           MASSHNTHSETAK +QILT    KTLQI+LESR+PCISSRNFSGEQVLSSPSSS      
Sbjct: 1   MASSHNTHSETAKVEQILTEFFPKTLQIILESRAPCISSRNFSGEQVLSSPSSSSSSSSS 60

Query: 61  -----------VRECSAALENIDLWRQSYHETMVVDVILV------DSVNASPRKDLVRN 120
                      +RECSA LENIDLWR +YHE MVVDVILV      DSVNASPRKDLVR+
Sbjct: 61  MRPRDKWFNLALRECSATLENIDLWRPNYHEPMVVDVILVQRQFGLDSVNASPRKDLVRH 120

Query: 121 LSLKEKYSL-FNCDKDEFGCDTKSEKIVERWMVHYETIKNRDS 137
           LSLKEKY L FN DKDEFG  TKSEK+VERWMVHYE+ KNRDS
Sbjct: 121 LSLKEKYPLSFNSDKDEFGSQTKSEKVVERWMVHYESRKNRDS 163

BLAST of MS016170 vs. NCBI nr
Match: XP_004148643.1 (autophagy-related protein 13b [Cucumis sativus] >KAE8647466.1 hypothetical protein Csa_003325 [Cucumis sativus])

HSP 1 Score: 208.4 bits (529), Expect = 4.0e-50
Identity = 118/163 (72.39%), Postives = 126/163 (77.30%), Query Frame = 0

Query: 1   MASSHNTHSETAKTDQILT---AKTLQIVLESRSPCISSRNFSGEQVLSSPSSS------ 60
           MASSHNTHSETAK +QILT    KTLQI+LESR+PCISSRNFSGEQVLSSPSSS      
Sbjct: 1   MASSHNTHSETAKVEQILTEFFPKTLQIILESRAPCISSRNFSGEQVLSSPSSSSSSSSS 60

Query: 61  -----------VRECSAALENIDLWRQSYHETMVVDVILV------DSVNASPRKDLVRN 120
                      +RECSA LENIDLWR +YHE MVVDVILV      DSV+ASPRKDLVRN
Sbjct: 61  MRPRDKWFNLALRECSATLENIDLWRPNYHEPMVVDVILVQRQFGLDSVSASPRKDLVRN 120

Query: 121 LSLKEKYSL-FNCDKDEFGCDTKSEKIVERWMVHYETIKNRDS 137
           LSLKEKY L FN DKDEFG  TKSEK+VERWMVHYE+ KNRDS
Sbjct: 121 LSLKEKYPLSFNSDKDEFGSQTKSEKVVERWMVHYESRKNRDS 163

BLAST of MS016170 vs. ExPASy Swiss-Prot
Match: F4J8V5 (Autophagy-related protein 13b OS=Arabidopsis thaliana OX=3702 GN=ATG13B PE=2 SV=1)

HSP 1 Score: 123.6 bits (309), Expect = 1.7e-27
Identity = 76/163 (46.63%), Postives = 107/163 (65.64%), Query Frame = 0

Query: 1   MASSH----NTHSETAKTDQIL---TAKTLQIVLESRSPCISSRNFSGEQVLSSPSSS-- 60
           M+SSH    N +SE AK +QI+    AK+L I+LESR+P +SSRNFSGEQ++ SPSSS  
Sbjct: 1   MSSSHNRSNNNNSEGAKAEQIIFEFFAKSLHIILESRTPFMSSRNFSGEQMICSPSSSSS 60

Query: 61  ---------------VRECSAALENIDLWRQSYHETMVVDVILVDSV---NASPRKDLVR 120
                          +REC AALE+ D+ R+S  E +VVDV+LV      + S +++L+R
Sbjct: 61  SSSSVRPRDKWFNLALRECPAALESFDIGRRSSLEPLVVDVVLVVRPLVGDQSGKRELIR 120

Query: 121 NLSLKEKYSLFNCDKDEFGCDTKSEKIVERWMVHYETIKNRDS 137
           N S K+  S +N D+DE GC+TK+E+I+ERW+V Y+  K R+S
Sbjct: 121 NFSGKDYQSGWNSDQDELGCETKNEQIIERWVVQYDNRKIRES 163

BLAST of MS016170 vs. ExPASy TrEMBL
Match: A0A6J1BUH2 (autophagy-related protein 13b OS=Momordica charantia OX=3673 GN=LOC111005657 PE=4 SV=1)

HSP 1 Score: 227.6 bits (579), Expect = 3.0e-56
Identity = 128/162 (79.01%), Postives = 132/162 (81.48%), Query Frame = 0

Query: 1   MASSHNTHSETAKTDQILT---AKTLQIVLESRSPCISSRNFSGEQVLSSPSSS------ 60
           MASSHNTHSETAKT+QILT   AKTLQIVLESRSPCISSRNFSGEQVLSSPSSS      
Sbjct: 1   MASSHNTHSETAKTEQILTEFFAKTLQIVLESRSPCISSRNFSGEQVLSSPSSSSSSSSS 60

Query: 61  -----------VRECSAALENIDLWRQSYHETMVVDVILV------DSVNASPRKDLVRN 120
                      +RECSAALENIDLWRQSYHE MVVDVILV      DSVNASPRKDLVRN
Sbjct: 61  MRPRDKWFNLALRECSAALENIDLWRQSYHEPMVVDVILVQRQFGLDSVNASPRKDLVRN 120

Query: 121 LSLKEKYSL-FNCDKDEFGCDTKSEKIVERWMVHYETIKNRD 136
           LSLK+KYSL FN DKDEFGCDTKSEK+VERWMVHYET KNRD
Sbjct: 121 LSLKDKYSLSFNSDKDEFGCDTKSEKVVERWMVHYETRKNRD 162

BLAST of MS016170 vs. ExPASy TrEMBL
Match: A0A0A0KFR2 (ATG13 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G484560 PE=4 SV=1)

HSP 1 Score: 208.4 bits (529), Expect = 1.9e-50
Identity = 118/163 (72.39%), Postives = 126/163 (77.30%), Query Frame = 0

Query: 1   MASSHNTHSETAKTDQILT---AKTLQIVLESRSPCISSRNFSGEQVLSSPSSS------ 60
           MASSHNTHSETAK +QILT    KTLQI+LESR+PCISSRNFSGEQVLSSPSSS      
Sbjct: 1   MASSHNTHSETAKVEQILTEFFPKTLQIILESRAPCISSRNFSGEQVLSSPSSSSSSSSS 60

Query: 61  -----------VRECSAALENIDLWRQSYHETMVVDVILV------DSVNASPRKDLVRN 120
                      +RECSA LENIDLWR +YHE MVVDVILV      DSV+ASPRKDLVRN
Sbjct: 61  MRPRDKWFNLALRECSATLENIDLWRPNYHEPMVVDVILVQRQFGLDSVSASPRKDLVRN 120

Query: 121 LSLKEKYSL-FNCDKDEFGCDTKSEKIVERWMVHYETIKNRDS 137
           LSLKEKY L FN DKDEFG  TKSEK+VERWMVHYE+ KNRDS
Sbjct: 121 LSLKEKYPLSFNSDKDEFGSQTKSEKVVERWMVHYESRKNRDS 163

BLAST of MS016170 vs. ExPASy TrEMBL
Match: A0A1S3B343 (autophagy-related protein 13b isoform X2 OS=Cucumis melo OX=3656 GN=LOC103485257 PE=4 SV=1)

HSP 1 Score: 208.4 bits (529), Expect = 1.9e-50
Identity = 118/163 (72.39%), Postives = 126/163 (77.30%), Query Frame = 0

Query: 1   MASSHNTHSETAKTDQILT---AKTLQIVLESRSPCISSRNFSGEQVLSSPSSS------ 60
           MASSHNTHSETAK +QILT    KTLQI+LESR+PCISSRNFSGEQVLSSPSSS      
Sbjct: 1   MASSHNTHSETAKVEQILTEFFPKTLQIILESRAPCISSRNFSGEQVLSSPSSSSSSSSS 60

Query: 61  -----------VRECSAALENIDLWRQSYHETMVVDVILV------DSVNASPRKDLVRN 120
                      +RECSA LENIDLWR +YHE MVVDVILV      DSVNASPRKDLVR+
Sbjct: 61  MRPRDKWFNLALRECSATLENIDLWRPNYHEPMVVDVILVQRQFGLDSVNASPRKDLVRH 120

Query: 121 LSLKEKYSL-FNCDKDEFGCDTKSEKIVERWMVHYETIKNRDS 137
           LSLKEKY L FN DKDEFG  TKSEK+VERWMVHYE+ KNRDS
Sbjct: 121 LSLKEKYPLSFNSDKDEFGSQTKSEKVVERWMVHYESRKNRDS 163

BLAST of MS016170 vs. ExPASy TrEMBL
Match: A0A1S3B2G3 (autophagy-related protein 13b isoform X1 OS=Cucumis melo OX=3656 GN=LOC103485257 PE=4 SV=1)

HSP 1 Score: 208.4 bits (529), Expect = 1.9e-50
Identity = 118/163 (72.39%), Postives = 126/163 (77.30%), Query Frame = 0

Query: 1   MASSHNTHSETAKTDQILT---AKTLQIVLESRSPCISSRNFSGEQVLSSPSSS------ 60
           MASSHNTHSETAK +QILT    KTLQI+LESR+PCISSRNFSGEQVLSSPSSS      
Sbjct: 1   MASSHNTHSETAKVEQILTEFFPKTLQIILESRAPCISSRNFSGEQVLSSPSSSSSSSSS 60

Query: 61  -----------VRECSAALENIDLWRQSYHETMVVDVILV------DSVNASPRKDLVRN 120
                      +RECSA LENIDLWR +YHE MVVDVILV      DSVNASPRKDLVR+
Sbjct: 61  MRPRDKWFNLALRECSATLENIDLWRPNYHEPMVVDVILVQRQFGLDSVNASPRKDLVRH 120

Query: 121 LSLKEKYSL-FNCDKDEFGCDTKSEKIVERWMVHYETIKNRDS 137
           LSLKEKY L FN DKDEFG  TKSEK+VERWMVHYE+ KNRDS
Sbjct: 121 LSLKEKYPLSFNSDKDEFGSQTKSEKVVERWMVHYESRKNRDS 163

BLAST of MS016170 vs. ExPASy TrEMBL
Match: A0A6J1IKL7 (autophagy-related protein 13b OS=Cucurbita maxima OX=3661 GN=LOC111477911 PE=4 SV=1)

HSP 1 Score: 203.4 bits (516), Expect = 6.2e-49
Identity = 113/163 (69.33%), Postives = 124/163 (76.07%), Query Frame = 0

Query: 1   MASSHNTHSETAKTDQILT---AKTLQIVLESRSPCISSRNFSGEQVLSSPSSS------ 60
           MASSHNTHSETAK +QILT    KTLQI+LESRSPCISSRNFSG+QVL SPSSS      
Sbjct: 1   MASSHNTHSETAKVEQILTEFFPKTLQIILESRSPCISSRNFSGDQVLCSPSSSSSSSSS 60

Query: 61  -----------VRECSAALENIDLWRQSYHETMVVDVILV------DSVNASPRKDLVRN 120
                      +RECSA LENIDLWR SYHE MV+DVILV      DSVNASPR+DLVRN
Sbjct: 61  MRPRDKWFNLALRECSATLENIDLWRPSYHEPMVIDVILVQREFGLDSVNASPRRDLVRN 120

Query: 121 LSLKEKYSL-FNCDKDEFGCDTKSEKIVERWMVHYETIKNRDS 137
           LSLKEK+    N DK+EFGC TKSEK+VERWMVHYE+ KNRD+
Sbjct: 121 LSLKEKHPFSLNSDKEEFGCQTKSEKVVERWMVHYESRKNRDT 163

BLAST of MS016170 vs. TAIR 10
Match: AT3G18770.1 (Autophagy-related protein 13 )

HSP 1 Score: 123.6 bits (309), Expect = 1.2e-28
Identity = 76/163 (46.63%), Postives = 107/163 (65.64%), Query Frame = 0

Query: 1   MASSH----NTHSETAKTDQIL---TAKTLQIVLESRSPCISSRNFSGEQVLSSPSSS-- 60
           M+SSH    N +SE AK +QI+    AK+L I+LESR+P +SSRNFSGEQ++ SPSSS  
Sbjct: 1   MSSSHNRSNNNNSEGAKAEQIIFEFFAKSLHIILESRTPFMSSRNFSGEQMICSPSSSSS 60

Query: 61  ---------------VRECSAALENIDLWRQSYHETMVVDVILVDSV---NASPRKDLVR 120
                          +REC AALE+ D+ R+S  E +VVDV+LV      + S +++L+R
Sbjct: 61  SSSSVRPRDKWFNLALRECPAALESFDIGRRSSLEPLVVDVVLVVRPLVGDQSGKRELIR 120

Query: 121 NLSLKEKYSLFNCDKDEFGCDTKSEKIVERWMVHYETIKNRDS 137
           N S K+  S +N D+DE GC+TK+E+I+ERW+V Y+  K R+S
Sbjct: 121 NFSGKDYQSGWNSDQDELGCETKNEQIIERWVVQYDNRKIRES 163

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022132934.16.3e-5679.01autophagy-related protein 13b [Momordica charantia] >XP_022132935.1 autophagy-re... [more]
XP_038882060.12.1e-5173.01autophagy-related protein 13b [Benincasa hispida][more]
XP_008441018.14.0e-5072.39PREDICTED: autophagy-related protein 13b isoform X2 [Cucumis melo][more]
XP_008441017.14.0e-5072.39PREDICTED: autophagy-related protein 13b isoform X1 [Cucumis melo][more]
XP_004148643.14.0e-5072.39autophagy-related protein 13b [Cucumis sativus] >KAE8647466.1 hypothetical prote... [more]
Match NameE-valueIdentityDescription
F4J8V51.7e-2746.63Autophagy-related protein 13b OS=Arabidopsis thaliana OX=3702 GN=ATG13B PE=2 SV=... [more]
Match NameE-valueIdentityDescription
A0A6J1BUH23.0e-5679.01autophagy-related protein 13b OS=Momordica charantia OX=3673 GN=LOC111005657 PE=... [more]
A0A0A0KFR21.9e-5072.39ATG13 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G484560 PE=4 ... [more]
A0A1S3B3431.9e-5072.39autophagy-related protein 13b isoform X2 OS=Cucumis melo OX=3656 GN=LOC103485257... [more]
A0A1S3B2G31.9e-5072.39autophagy-related protein 13b isoform X1 OS=Cucumis melo OX=3656 GN=LOC103485257... [more]
A0A6J1IKL76.2e-4969.33autophagy-related protein 13b OS=Cucurbita maxima OX=3661 GN=LOC111477911 PE=4 S... [more]
Match NameE-valueIdentityDescription
AT3G18770.11.2e-2846.63Autophagy-related protein 13 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036570HORMA domain superfamilyGENE3D3.30.900.10HORMA domaincoord: 2..136
e-value: 1.8E-12
score: 49.4
IPR018731Autophagy-related protein 13, N-terminalPFAMPF10033ATG13coord: 20..133
e-value: 6.0E-7
score: 29.4
NoneNo IPR availablePANTHERPTHR13430:SF4AUTOPHAGY-RELATED PROTEIN 13coord: 8..128
IPR040182Autophagy-related protein 13PANTHERPTHR13430UNCHARACTERIZEDcoord: 8..128

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS016170.1MS016170.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000045 autophagosome assembly
biological_process GO:0006914 autophagy
cellular_component GO:1990316 Atg1/ULK1 kinase complex