MS016166 (gene) Bitter gourd (TR) v1

BLAST of MS016166 vs. NCBI nr
Match: XP_022152560.1 (GDSL esterase/lipase 1-like [Momordica charantia])

HSP 1 Score: 754.2 bits (1946), Expect = 5.2e-214
Identity = 364/367 (99.18%), Postives = 364/367 (99.18%), Query Frame = 0

Query: 1   MASSWLSSSFLVFFIASFLSLQCCRCDDLKPPPKHVAFFIFGDSLFDVGNNNYINTTTSF 60
           MASSWLSSSFLVFFIASFLSLQCCRCDDLKPPPKHVAFFIFGDSLFDVGNNNYINTTTSF
Sbjct: 1   MASSWLSSSFLVFFIASFLSLQCCRCDDLKPPPKHVAFFIFGDSLFDVGNNNYINTTTSF 60

Query: 61  QANFLPYGETFFKFPTGRFSDGRLIPDFIAKYGNLPLIHPYLNPKNKFYLHGVNFASAGA 120
           QANFLPYGETFFKFPTGRFSDGRLIPDFIAKYGNLPLI PYLNPKNKFYLHGVNFASAGA
Sbjct: 61  QANFLPYGETFFKFPTGRFSDGRLIPDFIAKYGNLPLIRPYLNPKNKFYLHGVNFASAGA 120

Query: 121 GALVDTHQGFVIDLKTQLSYFNKVAKVLKEELGHAEAKALLSRAVYFISIGSNDYPFTTN 180
            ALVDTHQGFVIDLKTQLSYFNKVAKVLKEELGHAEAKALLSRAVYFISIGSNDYPFTTN
Sbjct: 121 DALVDTHQGFVIDLKTQLSYFNKVAKVLKEELGHAEAKALLSRAVYFISIGSNDYPFTTN 180

Query: 181 SSLFRSHSPQQYVDLVIGNLTTVIKGIHKNGGRKFAFLGVGPIGCVPTVKATLLQGKDEC 240
           SSLFRSHSPQQYVDLVIGNLTTVIKGIHKNGGRKFAFLGVGPIGCVPTVKATLLQGKDEC
Sbjct: 181 SSLFRSHSPQQYVDLVIGNLTTVIKGIHKNGGRKFAFLGVGPIGCVPTVKATLLQGKDEC 240

Query: 241 LEEITQLVKLHNNHLSKTLLQLGKKLEGFVYSYADFYATAIEVTNNPAKYDFKEGKVACC 300
           LEEITQLVKLHNNHLSKTLLQLGKKLEGFVYSYADFYA AIEVTNNPAKYDFKEGKVACC
Sbjct: 241 LEEITQLVKLHNNHLSKTLLQLGKKLEGFVYSYADFYAIAIEVTNNPAKYDFKEGKVACC 300

Query: 301 GSGPFRGYSSCGGRNGQEYELCGNPSEYLFFDGSHPSEKANQLFAQFLWNGSSEIIRPYN 360
           GSGPFRGYSSCGGRNGQEYELCGNPSEYLFFDGSHPSEKANQLFAQFLWNGSSEIIRPYN
Sbjct: 301 GSGPFRGYSSCGGRNGQEYELCGNPSEYLFFDGSHPSEKANQLFAQFLWNGSSEIIRPYN 360

Query: 361 LNVLFHL 368
           LNVLFHL
Sbjct: 361 LNVLFHL 367

BLAST of MS016166 vs. NCBI nr
Match: XP_023527011.1 (GDSL esterase/lipase 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 565.5 bits (1456), Expect = 3.4e-157
Identity = 279/368 (75.82%), Postives = 312/368 (84.78%), Query Frame = 0

Query: 3   SSWLSSSFLVFFIASFLSLQCCRCDDLKPPPKHVAFFIFGDSLFDVGNNNYINTTTSFQA 62
           SS+ SSSFLV+FI S LS+  C     K  P + A FIFGDSLFD GNNNYIN  TSF+A
Sbjct: 4   SSFPSSSFLVYFILSLLSIHSC-----KGHPNNAALFIFGDSLFDAGNNNYIN--TSFKA 63

Query: 63  NFLPYGETFFKFPTGRFSDGRLIPDFIAKYGNLPLIHPYLNPKNKFYLHGVNFASAGAGA 122
           NFLPYG++FFKFPTGRFSDGRLIPDFIAKY NLPLI PYL+P NK Y+HGVNFASAGAGA
Sbjct: 64  NFLPYGQSFFKFPTGRFSDGRLIPDFIAKYANLPLILPYLHPMNKIYVHGVNFASAGAGA 123

Query: 123 LVDTHQGFVIDLKTQLSYFNKVAKVLKEELGH-AEAKALLSRAVYFISIGSNDY--PFTT 182
           L DTHQG VIDLKTQLSYFNKV KV+ E +GH AEAKALLS+AVYFISIGSNDY  PFTT
Sbjct: 124 LADTHQGLVIDLKTQLSYFNKVTKVV-EGIGHPAEAKALLSKAVYFISIGSNDYIAPFTT 183

Query: 183 NSSLFRSHSPQQYVDLVIGNLTTVIKGIHKNGGRKFAFLGVGPIGCVPTVKATLLQGKDE 242
           NS+LF+SHSPQ+YV LVIGNLTT+IKGIHKNGGRKFAFLGVG IGCVP VKA  LQGKDE
Sbjct: 184 NSTLFQSHSPQEYVQLVIGNLTTIIKGIHKNGGRKFAFLGVGDIGCVPLVKAVFLQGKDE 243

Query: 243 CLEEITQLVKLHNNHLSKTLLQLGKKLEGFVYSYADFYATAIEVTNNPAKYDFKEGKVAC 302
           C E ITQLV LHN HL KTL  L K+LEGFVYSYAD Y +A +++NNPAKY FKEGK AC
Sbjct: 244 CFENITQLVTLHNKHLYKTLQHLEKELEGFVYSYADLYTSANQISNNPAKYGFKEGKAAC 303

Query: 303 CGSGPFRGYSSCGGRNGQEYELCGNPSEYLFFDGSHPSEKANQLFAQFLWNGSSEIIRPY 362
           CGSGPFRGY SCGGR+G+EYELC NP+EYLF+D +HP+E+ANQL A++LWNGSS  I+PY
Sbjct: 304 CGSGPFRGYFSCGGRDGEEYELCSNPNEYLFYDATHPTERANQLVAEWLWNGSSGSIKPY 363

Query: 363 NLNVLFHL 368
           NL  LF++
Sbjct: 364 NLKALFNV 363

BLAST of MS016166 vs. NCBI nr
Match: XP_022956141.1 (GDSL esterase/lipase 1-like [Cucurbita moschata])

HSP 1 Score: 564.3 bits (1453), Expect = 7.7e-157
Identity = 279/368 (75.82%), Postives = 314/368 (85.33%), Query Frame = 0

Query: 3   SSWLSSSFLVFFIASFLSLQCCRCDDLKPPPKHVAFFIFGDSLFDVGNNNYINTTTSFQA 62
           SS+ SS+FLV+FI S LS+  C+       PK+ A FIFGDSLFD GNNNYIN  TSF+A
Sbjct: 4   SSFPSSTFLVYFIFSLLSIHSCK-GHPNTWPKNAALFIFGDSLFDAGNNNYIN--TSFKA 63

Query: 63  NFLPYGETFFKFPTGRFSDGRLIPDFIAKYGNLPLIHPYLNPKNKFYLHGVNFASAGAGA 122
           NFLPYG++FFKFPTGRFSDGRLIPDFIAKY NLPLI PYL+P NK Y+HGVNFASAGAGA
Sbjct: 64  NFLPYGQSFFKFPTGRFSDGRLIPDFIAKYANLPLILPYLHPMNKNYVHGVNFASAGAGA 123

Query: 123 LVDTHQGFVIDLKTQLSYFNKVAKVLKEELGH-AEAKALLSRAVYFISIGSNDY--PFTT 182
           L +THQG VIDLKTQLSYFNKV KV+ E +GH AEAKALLS+AVYFISIGSNDY  PFTT
Sbjct: 124 LAETHQGLVIDLKTQLSYFNKVTKVV-EGIGHPAEAKALLSKAVYFISIGSNDYIAPFTT 183

Query: 183 NSSLFRSHSPQQYVDLVIGNLTTVIKGIHKNGGRKFAFLGVGPIGCVPTVKATLLQGKDE 242
           NS+LF+SHSPQ+YV+LVIGNLTTVIKGIHKNGGRKFAFLGVG IGCVP VKA  LQGKDE
Sbjct: 184 NSTLFQSHSPQEYVELVIGNLTTVIKGIHKNGGRKFAFLGVGDIGCVPLVKAVFLQGKDE 243

Query: 243 CLEEITQLVKLHNNHLSKTLLQLGKKLEGFVYSYADFYATAIEVTNNPAKYDFKEGKVAC 302
           C E ITQLV LHN HL KTL  L K+LEGFVYSYAD Y +A +++NNPAKY FKEGK AC
Sbjct: 244 CFENITQLVTLHNKHLYKTLQHLEKELEGFVYSYADLYTSANQISNNPAKYGFKEGKAAC 303

Query: 303 CGSGPFRGYSSCGGRNGQEYELCGNPSEYLFFDGSHPSEKANQLFAQFLWNGSSEIIRPY 362
           CGSGPFRGY SCGGR+GQEYELC NP+EYLF+D +HP+E+ANQL A++LWNGSS  I+PY
Sbjct: 304 CGSGPFRGYFSCGGRDGQEYELCSNPNEYLFYDATHPTERANQLVAEWLWNGSSGSIKPY 363

Query: 363 NLNVLFHL 368
           NL  LF++
Sbjct: 364 NLKALFNV 367

BLAST of MS016166 vs. NCBI nr
Match: XP_008460334.1 (PREDICTED: GDSL esterase/lipase 2-like [Cucumis melo] >KAA0053543.1 GDSL esterase/lipase 2-like [Cucumis melo var. makuwa] >TYK19137.1 GDSL esterase/lipase 2-like [Cucumis melo var. makuwa])

HSP 1 Score: 556.6 bits (1433), Expect = 1.6e-154
Identity = 273/372 (73.39%), Postives = 307/372 (82.53%), Query Frame = 0

Query: 1   MASSWLSSSFLVFFIASFLSLQCCRCDDLKPPPK-HVAFFIFGDSLFDVGNNNYINTTTS 60
           MA+S   SS LV+FI   + +Q C+ +   P PK HV  FIFGDSLFD GNNNYIN  T 
Sbjct: 1   MAASKFPSSLLVYFIVIAVCMQICKGNPKIPLPKLHVPLFIFGDSLFDAGNNNYIN--TP 60

Query: 61  FQANFLPYGETFFKFPTGRFSDGRLIPDFIAKYGNLPLIHPYLNPKNKFYLHGVNFASAG 120
           FQ+NF PYGETFF FPTGRFSDGRLIPDFIAKY NLP IHPYLNP NK YLHGVNFASAG
Sbjct: 61  FQSNFWPYGETFFNFPTGRFSDGRLIPDFIAKYANLPFIHPYLNPTNKNYLHGVNFASAG 120

Query: 121 AGALVDTHQGFVIDLKTQLSYFNKVAKVLKEELGHAE--AKALLSRAVYFISIGSNDY-- 180
           AGALV+T QGFVIDLKTQLSYFNKV KV +E  G  E  AKALLSRAVY I+IGSNDY  
Sbjct: 121 AGALVETQQGFVIDLKTQLSYFNKVRKVFEEVGGGHEGGAKALLSRAVYLINIGSNDYLA 180

Query: 181 PFTTNSSLFRSHSPQQYVDLVIGNLTTVIKGIHKNGGRKFAFLGVGPIGCVPTVKATLLQ 240
           PF TNS+LF+SHSPQQYVDLVIGNLTTVIKGI+KNGGRKFAF GVGP+GC P VKA +LQ
Sbjct: 181 PFLTNSTLFQSHSPQQYVDLVIGNLTTVIKGIYKNGGRKFAFFGVGPLGCFPLVKAVILQ 240

Query: 241 GKDECLEEITQLVKLHNNHLSKTLLQLGKKLEGFVYSYADFYATAIEVTNNPAKYDFKEG 300
           GKDEC +EIT+L KLHN HL KTLL+LGKKLEGFVY+Y D +   IE+ NNPAKY  KEG
Sbjct: 241 GKDECFDEITELAKLHNKHLYKTLLRLGKKLEGFVYTYVDSFTVVIELLNNPAKYGLKEG 300

Query: 301 KVACCGSGPFRGYSSCGGRNGQEYELCGNPSEYLFFDGSHPSEKANQLFAQFLWNGSSEI 360
           KVACCGSGPFRGY SCGGRNG+EY+LC NPS++LFFD +H ++KANQL+A+ LWNG+ + 
Sbjct: 301 KVACCGSGPFRGYFSCGGRNGEEYKLCNNPSQHLFFDATHFTDKANQLYAELLWNGNLQT 360

Query: 361 IRPYNLNVLFHL 368
           I+PYNL  LFH+
Sbjct: 361 IKPYNLKTLFHV 370

BLAST of MS016166 vs. NCBI nr
Match: XP_022956142.1 (GDSL esterase/lipase 2-like [Cucurbita moschata])

HSP 1 Score: 555.8 bits (1431), Expect = 2.7e-154
Identity = 275/370 (74.32%), Postives = 314/370 (84.86%), Query Frame = 0

Query: 1   MASSWLSSSFLVFFIASFLSLQCCRCDDLKPPPKHVAFFIFGDSLFDVGNNNYINTTTSF 60
           MASS   SSFLV+FI S LS+  C     K  P   A FIFGDSLFD GNNNYIN  TSF
Sbjct: 1   MASSCFPSSFLVYFILSLLSIHNC-----KGHPNSAALFIFGDSLFDAGNNNYIN--TSF 60

Query: 61  QANFLPYGETFFKFPTGRFSDGRLIPDFIAKYGNLPLIHPYLNPKNKFYLHGVNFASAGA 120
           +ANF+PYG++FFKFPTGRFSDGRLIPDFIAKY NLPLI PYL+P NK Y+HGVNFASAGA
Sbjct: 61  KANFVPYGQSFFKFPTGRFSDGRLIPDFIAKYANLPLILPYLHPMNKNYVHGVNFASAGA 120

Query: 121 GALVDTHQGFVIDLKTQLSYFNKVAKVLKEELGH-AEAKALLSRAVYFISIGSNDY--PF 180
           GAL +THQG VIDLKTQLSYFNKV KV+ E +GH AEAKALLS+AVYFISIGSNDY  PF
Sbjct: 121 GALAETHQGLVIDLKTQLSYFNKVTKVV-EGIGHPAEAKALLSKAVYFISIGSNDYIAPF 180

Query: 181 TTNSSLFRSHSPQQYVDLVIGNLTTVIKGIHKNGGRKFAFLGVGPIGCVPTVKATLLQGK 240
           TTNS+LF+SHSPQ+YV+LVIGNLTTVIKGIHKNGGRKFAFLGVG IGCVP VKA LLQGK
Sbjct: 181 TTNSTLFQSHSPQEYVELVIGNLTTVIKGIHKNGGRKFAFLGVGDIGCVPLVKAVLLQGK 240

Query: 241 DECLEEITQLVKLHNNHLSKTLLQLGKKLEGFVYSYADFYATAIEVTNNPAKYDFKEGKV 300
           DEC E ITQLV LHN HL KTL  L K+LEGFVYSYAD Y++A +++NNP+KY FKEGK 
Sbjct: 241 DECSENITQLVTLHNKHLYKTLQHLEKELEGFVYSYADLYSSANQISNNPSKYGFKEGKA 300

Query: 301 ACCGSGPFRGYSSCGGRNGQEYELCGNPSEYLFFDGSHPSEKANQLFAQFLWNGSSEIIR 360
           ACCGSGPFRG+ SCGGR+G+EYELC NP+EYLF+D +H ++KA+QL A+++WNGSS+ I+
Sbjct: 301 ACCGSGPFRGFFSCGGRDGEEYELCSNPNEYLFYDSAHLTQKASQLVAEWVWNGSSDSIK 360

Query: 361 PYNLNVLFHL 368
           PYNL  LF++
Sbjct: 361 PYNLKALFNV 362

BLAST of MS016166 vs. ExPASy Swiss-Prot
Match: Q9FLN0 (GDSL esterase/lipase 1 OS=Arabidopsis thaliana OX=3702 GN=GLIP1 PE=1 SV=1)

HSP 1 Score: 364.4 bits (934), Expect = 1.5e-99
Identity = 189/372 (50.81%), Postives = 244/372 (65.59%), Query Frame = 0

Query: 3   SSWLSSSFLVF-FIASFLSLQCCRCDDLKPPPKHVAFFIFGDSLFDVGNNNYINTTTSFQ 62
           S  +S +FL +  I S  S+ C   D+        A F+FGDS+FD GNNNYI+T +S +
Sbjct: 4   SQLVSITFLAYTIIISIGSINC--IDNNNLVTNQSALFVFGDSVFDAGNNNYIDTLSSVR 63

Query: 63  ANFLPYGETFFKFPTGRFSDGRLIPDFIAKYGNLPLIHPYLNP--KNKFYLHGVNFASAG 122
           +N+ PYG+T FK PTGR SDGRLIPDFIA+Y  LPLI P L P   N  + +GVNFAS G
Sbjct: 64  SNYWPYGQTTFKSPTGRVSDGRLIPDFIAEYAWLPLIPPNLQPFNGNSQFAYGVNFASGG 123

Query: 123 AGALVDTHQGFVIDLKTQLSYFNKVAKVLKEELGHAEAKALLSRAVYFISIGSND--YPF 182
           AGALV T  G VI+L+TQL+ F KV ++L+ +LG AE K ++SRAVY   IG ND  YPF
Sbjct: 124 AGALVGTFSGLVINLRTQLNNFKKVEEMLRSKLGDAEGKRVISRAVYLFHIGLNDYQYPF 183

Query: 183 TTNSSLFRSHSPQQYVDLVIGNLTTVIKGIHKNGGRKFAFLGVGPIGCVPTVKATLLQGK 242
           TTNSSLF+S S ++YVD V+GN+T V K ++  GGRKF  L  GP  C P          
Sbjct: 184 TTNSSLFQSISNEKYVDYVVGNMTDVFKEVYNLGGRKFGILNTGPYDCAPASLVIDQTKI 243

Query: 243 DECLEEITQLVKLHNNHLSKTLLQLGKKLEGFVYSYADFYATAIEVTNNPAKYDFKEGKV 302
             C + +T+L+ +HN  L   L +L  +L GF Y+  D++ +  E  N+P+KY FKEGK 
Sbjct: 244 RSCFQPVTELINMHNEKLLNGLRRLNHELSGFKYALHDYHTSLSERMNDPSKYGFKEGKK 303

Query: 303 ACCGSGPFRGYSSCGGRNG--QEYELCGNPSEYLFFDGSHPSEKANQLFAQFLWNGSSEI 362
           ACCGSGP RG ++CGGR G  Q YELC N ++YLFFD  H +EKAN+  A+ +W+G + I
Sbjct: 304 ACCGSGPLRGINTCGGRMGLSQSYELCENVTDYLFFDPFHLTEKANRQIAELIWSGPTNI 363

Query: 363 IRPYNLNVLFHL 368
             PYNL  LF L
Sbjct: 364 TGPYNLKALFEL 373

BLAST of MS016166 vs. ExPASy Swiss-Prot
Match: Q9SYF0 (GDSL esterase/lipase 2 OS=Arabidopsis thaliana OX=3702 GN=GLIP2 PE=2 SV=1)

HSP 1 Score: 362.8 bits (930), Expect = 4.4e-99
Identity = 183/370 (49.46%), Postives = 244/370 (65.95%), Query Frame = 0

Query: 8   SSFLVFFIASFL----SLQCCRCDDLKPPPKHVAFFIFGDSLFDVGNNNYINTTTSFQAN 67
           S+ ++FF  + +    S+ C   ++        A F+FGDS+FD GNNNYI+T  SF++N
Sbjct: 6   STLIIFFAYTTIILIGSINCRDNNNNNLVTNQSALFVFGDSVFDAGNNNYIDTLPSFRSN 65

Query: 68  FLPYGETFFKFPTGRFSDGRLIPDFIAKYGNLPLIHPYLNPKN--KFYLHGVNFASAGAG 127
           + PYG+T FKFPTGR SDGR IPDFIA+Y  LPLI  YL P N    + +GV+FASAGAG
Sbjct: 66  YWPYGQTTFKFPTGRVSDGRTIPDFIAEYAWLPLIPAYLQPSNGKNQFPYGVSFASAGAG 125

Query: 128 ALVDTHQGFVIDLKTQLSYFNKVAKVLKEELGHAEAKALLSRAVYFISIGSND--YPFTT 187
           ALV T  G VI+LK+QL+ F KV K+L+  LG A+ K ++SRAVY   IG ND  YPF+T
Sbjct: 126 ALVGTFPGMVINLKSQLNNFKKVEKLLRSTLGEAQGKMVISRAVYLFHIGVNDYQYPFST 185

Query: 188 NSSLFRSHSPQQYVDLVIGNLTTVIKGIHKNGGRKFAFLGVGPIGCVPTVKATLLQGKDE 247
           NSS+F+S   + YVD V+GN T VIK ++K GGRKF FL +G   C P            
Sbjct: 186 NSSIFQSSPQEIYVDFVVGNTTAVIKEVYKIGGRKFGFLNMGAYDCAPASLIIDQTKIGT 245

Query: 248 CLEEITQLVKLHNNHLSKTLLQLGKKLEGFVYSYADFYATAIEVTNNPAKYDFKEGKVAC 307
           C + +T+L+ LHN  L   L +L ++L GF Y+  D++ +     NNP+KY FKEGK+AC
Sbjct: 246 CFKPVTELINLHNEKLESGLRRLERELSGFKYALHDYHTSLSVRMNNPSKYGFKEGKMAC 305

Query: 308 CGSGPFRGYSSCGGRNG--QEYELCGNPSEYLFFDGSHPSEKANQLFAQFLWNGSSEIIR 367
           CG+GP RG ++CGGR G  Q YELC   ++YLFFD  H +EKA+Q  A+ +W+G + + +
Sbjct: 306 CGTGPLRGINTCGGRMGVSQSYELCEKVTDYLFFDHFHLTEKAHQQIAELIWSGPTNVTK 365

BLAST of MS016166 vs. ExPASy Swiss-Prot
Match: Q9SSA7 (GDSL esterase/lipase 5 OS=Arabidopsis thaliana OX=3702 GN=GLIP5 PE=2 SV=2)

HSP 1 Score: 344.0 bits (881), Expect = 2.1e-93
Identity = 184/371 (49.60%), Postives = 242/371 (65.23%), Query Frame = 0

Query: 8   SSFLVFFIAS---FLSLQCCRCDDLKPPPKHVAFFIFGDSLFDVGNNNYINTTTSFQANF 67
           SSF+ F ++S   FL+ +              A F+FGDS  D GNNNYINTTT  QANF
Sbjct: 16  SSFIFFIVSSTILFLAGKSSAKISHNGDNNVTALFLFGDSFLDAGNNNYINTTTLDQANF 75

Query: 68  LPYGETFFKFPTGRFSDGRLIPDFIAKYGNLPLIHPYLNPKN-KFYLHGVNFASAGAGAL 127
            PYG+TFF  PTGRFSDGRLI DFIA+Y NLPLI P+L P N +  L+GVNFASAGAGAL
Sbjct: 76  PPYGQTFFGLPTGRFSDGRLISDFIAEYANLPLIPPFLEPGNSQKKLYGVNFASAGAGAL 135

Query: 128 VDTHQGFVIDLKTQLSYFNKVAKVLKEELGHAEAKALLSRAVYFISIGSNDYP--FTTNS 187
           V+T QG VI+L+TQL ++ KV ++ +   G  E+K  +SRAVY ISIGSNDY   F TN 
Sbjct: 136 VETFQGSVINLRTQLDHYKKVERLWRTNFGKEESKKRISRAVYLISIGSNDYSSIFLTNQ 195

Query: 188 SLFRSHSPQQYVDLVIGNLTTVIKGIHKNGGRKFAFLGVGPIGCVPTVKATLLQGKDECL 247
           SL  S S  Q+VD+VIGNLTT I  I+K GGRKF FL V  +GC P ++    +  D CL
Sbjct: 196 SLPISMS--QHVDIVIGNLTTFIHEIYKIGGRKFGFLNVPDLGCFPALRILQPKNDDSCL 255

Query: 248 EEITQLVKLHNNHLSKTLLQLGKKLEGFVYSYADFYATAIEVTNNPAKYDFKEGKVACCG 307
            + ++L  +HN  L+  L Q+ ++++GF +S  D   +      +P+K+ FKEG+ ACCG
Sbjct: 256 RDASRLASMHNRALTNLLFQMQRQVKGFKFSLFDMNKSLRLRMQHPSKFGFKEGEEACCG 315

Query: 308 SGPFRGYSSCGG-RNGQEYELCGNPSEYLFFDGSHPSEKANQLFAQFLWNG----SSEII 367
           +G +RG  SCGG R  +EY+LC NP +Y+F+D  H ++     FA  +WNG     S ++
Sbjct: 316 TGKWRGVFSCGGKRIVKEYQLCENPKDYIFWDSLHLTQNTYNQFANLIWNGGHMSDSLVV 375

BLAST of MS016166 vs. ExPASy Swiss-Prot
Match: Q9LJP1 (GDSL esterase/lipase 4 OS=Arabidopsis thaliana OX=3702 GN=GLIP4 PE=2 SV=2)

HSP 1 Score: 342.4 bits (877), Expect = 6.2e-93
Identity = 177/379 (46.70%), Postives = 240/379 (63.32%), Query Frame = 0

Query: 1   MASSWLSSSFLVFFIASF-LSLQCCRC-DDLKPPPKHVAFFIFGDSLFDVGNNNYINTTT 60
           MAS   +S  ++ FI +  LS+    C +DLK      A F FGDSLF+ GNNNY ++ +
Sbjct: 1   MASPRFNSIIIILFICTISLSIVSISCKEDLK--TNQAALFAFGDSLFEAGNNNYFDSIS 60

Query: 61  SFQANFLPYGETFFKFPTGRFSDGRLIPDFIAKYGNLPLIHPYLNP--KNKFYLHGVNFA 120
           SF++NF PYG+T FKFPTGR SDGR++ DFIA+Y  LPLI P L P   N    +G+NFA
Sbjct: 61  SFRSNFWPYGKTTFKFPTGRVSDGRIMIDFIAEYAWLPLIPPNLQPGYSNSQLTYGLNFA 120

Query: 121 SAGAGALVDTHQGFVI----DLKTQLSYFNKVAKVLKEELGHAEAKALLSRAVYFISIGS 180
           +  AG    T  G V     DL TQL+ F  V K L+  LG AEA+ ++S+AVY   IG+
Sbjct: 121 TTAAGVFAGTFPGSVTNLSKDLGTQLNNFKNVEKTLRSNLGDAEARRVISKAVYLFHIGA 180

Query: 181 ND--YPFTTNSSLFRSHSPQQYVDLVIGNLTTVIKGIHKNGGRKFAFLGVGPIGCVPTVK 240
           ND  YPF  N+S F + + ++++D VIGN TTVI+ ++K G RKF FL +GP GC P+  
Sbjct: 181 NDYQYPFFANTSTFSNTTKERFIDFVIGNTTTVIEELYKLGARKFGFLSLGPFGCTPSAL 240

Query: 241 ATLLQGKDECLEEITQLVKLHNNHLSKTLLQLGKKLEGFVYSYADFYATAIEVTNNPAKY 300
                    C E +T+L+ LHN    K L +L ++L GF Y+  DF+ +  +  NNP++Y
Sbjct: 241 IINSTKIGSCFEPVTELINLHNQEFPKVLRRLERRLSGFKYALHDFHTSLSQRINNPSRY 300

Query: 301 DFKEGKVACCGSGPFRGYSSCGGRNG--QEYELCGNPSEYLFFDGSHPSEKANQLFAQFL 360
            FKEG++ACCGSGP RG ++CG RNG  Q Y+LC N  +Y+FFD SH +E A+Q  A+ +
Sbjct: 301 GFKEGEMACCGSGPLRGINTCGFRNGPSQGYKLCENADDYVFFDPSHLTETAHQQIAELI 360

Query: 361 WNGSSEIIRPYNLNVLFHL 368
           W+G   +  PYNL  LF L
Sbjct: 361 WSGPPNVTAPYNLKTLFRL 377

BLAST of MS016166 vs. ExPASy Swiss-Prot
Match: H6U1I8 (GDSL lipase OS=Tanacetum cinerariifolium OX=118510 GN=GLIP PE=1 SV=1)

HSP 1 Score: 342.0 bits (876), Expect = 8.1e-93
Identity = 169/329 (51.37%), Postives = 221/329 (67.17%), Query Frame = 0

Query: 37  AFFIFGDSLFDVGNNNYINTTTSFQANFLPYGETFFKFPTGRFSDGRLIPDFIAKYGNLP 96
           A FIFGDS+FD GNNN+INT  +F+ANF PYG+++F  PTGRFSDGR+IPDFIA+Y +LP
Sbjct: 33  ALFIFGDSVFDPGNNNHINTHVNFKANFWPYGQSYFSSPTGRFSDGRIIPDFIAEYASLP 92

Query: 97  LIHPYLNPKNKFYLHGVNFASAGAGALVDTHQGFVIDLKTQLSYFNKVAKVLKEELGHAE 156
           +I  YL P N F  HG NFASAGAGAL+ +H G  + L+TQL YF  +    ++ LG  +
Sbjct: 93  IIPAYLEPNNDF-THGANFASAGAGALIASHAGLAVGLQTQLRYFGDLVDHYRQNLGDIK 152

Query: 157 AKALLSRAVYFISIGSNDYPFTTNSSLFRSHSPQQYVDLVIGNLTTVIKGIHKNGGRKFA 216
           ++ LLS AVY  S G NDY      S +  ++ +QYVD+VIGN+T VIKGI++ GGRKF 
Sbjct: 153 SRQLLSDAVYLFSCGGNDY-----QSPYYPYTQEQYVDIVIGNMTNVIKGIYEKGGRKFG 212

Query: 217 FLGVGPIGCVPTVKATLLQGKDECLEEITQLVKLHNNHLSKTLLQLGKKLEGFVYSYADF 276
            + V  IGC P ++A   Q  + C  E+ +L +LHN   +K L QL K+LEGFVY+  D 
Sbjct: 213 VVNVPLIGCWPGMRAK--QPGNTCNTEVDELTRLHNQAFAKRLEQLEKQLEGFVYAKFDL 272

Query: 277 YATAIEVTNNPAKYDFKEGKVACCGSGPFRGYSSCGGRNGQEYELCGNPSEYLFFDGSHP 336
               +    NP+KY FKEG+ ACCGSGPF G   CG    +E+ LC N +EY FFD  HP
Sbjct: 273 STAILNRMKNPSKYGFKEGESACCGSGPFGGNYDCG--RIKEFGLCDNATEYFFFDPFHP 332

Query: 337 SEKANQLFAQFLWNGSSEIIRPYNLNVLF 366
           +E A++ FA+  W+G S + +PYNL  LF
Sbjct: 333 NELASRQFAEMFWDGDSMVTQPYNLKALF 351

BLAST of MS016166 vs. ExPASy TrEMBL
Match: A0A6J1DEA0 (GDSL esterase/lipase 1-like OS=Momordica charantia OX=3673 GN=LOC111020258 PE=3 SV=1)

HSP 1 Score: 754.2 bits (1946), Expect = 2.5e-214
Identity = 364/367 (99.18%), Postives = 364/367 (99.18%), Query Frame = 0

Query: 1   MASSWLSSSFLVFFIASFLSLQCCRCDDLKPPPKHVAFFIFGDSLFDVGNNNYINTTTSF 60
           MASSWLSSSFLVFFIASFLSLQCCRCDDLKPPPKHVAFFIFGDSLFDVGNNNYINTTTSF
Sbjct: 1   MASSWLSSSFLVFFIASFLSLQCCRCDDLKPPPKHVAFFIFGDSLFDVGNNNYINTTTSF 60

Query: 61  QANFLPYGETFFKFPTGRFSDGRLIPDFIAKYGNLPLIHPYLNPKNKFYLHGVNFASAGA 120
           QANFLPYGETFFKFPTGRFSDGRLIPDFIAKYGNLPLI PYLNPKNKFYLHGVNFASAGA
Sbjct: 61  QANFLPYGETFFKFPTGRFSDGRLIPDFIAKYGNLPLIRPYLNPKNKFYLHGVNFASAGA 120

Query: 121 GALVDTHQGFVIDLKTQLSYFNKVAKVLKEELGHAEAKALLSRAVYFISIGSNDYPFTTN 180
            ALVDTHQGFVIDLKTQLSYFNKVAKVLKEELGHAEAKALLSRAVYFISIGSNDYPFTTN
Sbjct: 121 DALVDTHQGFVIDLKTQLSYFNKVAKVLKEELGHAEAKALLSRAVYFISIGSNDYPFTTN 180

Query: 181 SSLFRSHSPQQYVDLVIGNLTTVIKGIHKNGGRKFAFLGVGPIGCVPTVKATLLQGKDEC 240
           SSLFRSHSPQQYVDLVIGNLTTVIKGIHKNGGRKFAFLGVGPIGCVPTVKATLLQGKDEC
Sbjct: 181 SSLFRSHSPQQYVDLVIGNLTTVIKGIHKNGGRKFAFLGVGPIGCVPTVKATLLQGKDEC 240

Query: 241 LEEITQLVKLHNNHLSKTLLQLGKKLEGFVYSYADFYATAIEVTNNPAKYDFKEGKVACC 300
           LEEITQLVKLHNNHLSKTLLQLGKKLEGFVYSYADFYA AIEVTNNPAKYDFKEGKVACC
Sbjct: 241 LEEITQLVKLHNNHLSKTLLQLGKKLEGFVYSYADFYAIAIEVTNNPAKYDFKEGKVACC 300

Query: 301 GSGPFRGYSSCGGRNGQEYELCGNPSEYLFFDGSHPSEKANQLFAQFLWNGSSEIIRPYN 360
           GSGPFRGYSSCGGRNGQEYELCGNPSEYLFFDGSHPSEKANQLFAQFLWNGSSEIIRPYN
Sbjct: 301 GSGPFRGYSSCGGRNGQEYELCGNPSEYLFFDGSHPSEKANQLFAQFLWNGSSEIIRPYN 360

Query: 361 LNVLFHL 368
           LNVLFHL
Sbjct: 361 LNVLFHL 367

BLAST of MS016166 vs. ExPASy TrEMBL
Match: A0A6J1GVR6 (GDSL esterase/lipase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111457921 PE=3 SV=1)

HSP 1 Score: 564.3 bits (1453), Expect = 3.7e-157
Identity = 279/368 (75.82%), Postives = 314/368 (85.33%), Query Frame = 0

Query: 3   SSWLSSSFLVFFIASFLSLQCCRCDDLKPPPKHVAFFIFGDSLFDVGNNNYINTTTSFQA 62
           SS+ SS+FLV+FI S LS+  C+       PK+ A FIFGDSLFD GNNNYIN  TSF+A
Sbjct: 4   SSFPSSTFLVYFIFSLLSIHSCK-GHPNTWPKNAALFIFGDSLFDAGNNNYIN--TSFKA 63

Query: 63  NFLPYGETFFKFPTGRFSDGRLIPDFIAKYGNLPLIHPYLNPKNKFYLHGVNFASAGAGA 122
           NFLPYG++FFKFPTGRFSDGRLIPDFIAKY NLPLI PYL+P NK Y+HGVNFASAGAGA
Sbjct: 64  NFLPYGQSFFKFPTGRFSDGRLIPDFIAKYANLPLILPYLHPMNKNYVHGVNFASAGAGA 123

Query: 123 LVDTHQGFVIDLKTQLSYFNKVAKVLKEELGH-AEAKALLSRAVYFISIGSNDY--PFTT 182
           L +THQG VIDLKTQLSYFNKV KV+ E +GH AEAKALLS+AVYFISIGSNDY  PFTT
Sbjct: 124 LAETHQGLVIDLKTQLSYFNKVTKVV-EGIGHPAEAKALLSKAVYFISIGSNDYIAPFTT 183

Query: 183 NSSLFRSHSPQQYVDLVIGNLTTVIKGIHKNGGRKFAFLGVGPIGCVPTVKATLLQGKDE 242
           NS+LF+SHSPQ+YV+LVIGNLTTVIKGIHKNGGRKFAFLGVG IGCVP VKA  LQGKDE
Sbjct: 184 NSTLFQSHSPQEYVELVIGNLTTVIKGIHKNGGRKFAFLGVGDIGCVPLVKAVFLQGKDE 243

Query: 243 CLEEITQLVKLHNNHLSKTLLQLGKKLEGFVYSYADFYATAIEVTNNPAKYDFKEGKVAC 302
           C E ITQLV LHN HL KTL  L K+LEGFVYSYAD Y +A +++NNPAKY FKEGK AC
Sbjct: 244 CFENITQLVTLHNKHLYKTLQHLEKELEGFVYSYADLYTSANQISNNPAKYGFKEGKAAC 303

Query: 303 CGSGPFRGYSSCGGRNGQEYELCGNPSEYLFFDGSHPSEKANQLFAQFLWNGSSEIIRPY 362
           CGSGPFRGY SCGGR+GQEYELC NP+EYLF+D +HP+E+ANQL A++LWNGSS  I+PY
Sbjct: 304 CGSGPFRGYFSCGGRDGQEYELCSNPNEYLFYDATHPTERANQLVAEWLWNGSSGSIKPY 363

Query: 363 NLNVLFHL 368
           NL  LF++
Sbjct: 364 NLKALFNV 367

BLAST of MS016166 vs. ExPASy TrEMBL
Match: A0A5D3D6E4 (GDSL esterase/lipase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold522G00610 PE=3 SV=1)

HSP 1 Score: 556.6 bits (1433), Expect = 7.7e-155
Identity = 273/372 (73.39%), Postives = 307/372 (82.53%), Query Frame = 0

Query: 1   MASSWLSSSFLVFFIASFLSLQCCRCDDLKPPPK-HVAFFIFGDSLFDVGNNNYINTTTS 60
           MA+S   SS LV+FI   + +Q C+ +   P PK HV  FIFGDSLFD GNNNYIN  T 
Sbjct: 1   MAASKFPSSLLVYFIVIAVCMQICKGNPKIPLPKLHVPLFIFGDSLFDAGNNNYIN--TP 60

Query: 61  FQANFLPYGETFFKFPTGRFSDGRLIPDFIAKYGNLPLIHPYLNPKNKFYLHGVNFASAG 120
           FQ+NF PYGETFF FPTGRFSDGRLIPDFIAKY NLP IHPYLNP NK YLHGVNFASAG
Sbjct: 61  FQSNFWPYGETFFNFPTGRFSDGRLIPDFIAKYANLPFIHPYLNPTNKNYLHGVNFASAG 120

Query: 121 AGALVDTHQGFVIDLKTQLSYFNKVAKVLKEELGHAE--AKALLSRAVYFISIGSNDY-- 180
           AGALV+T QGFVIDLKTQLSYFNKV KV +E  G  E  AKALLSRAVY I+IGSNDY  
Sbjct: 121 AGALVETQQGFVIDLKTQLSYFNKVRKVFEEVGGGHEGGAKALLSRAVYLINIGSNDYLA 180

Query: 181 PFTTNSSLFRSHSPQQYVDLVIGNLTTVIKGIHKNGGRKFAFLGVGPIGCVPTVKATLLQ 240
           PF TNS+LF+SHSPQQYVDLVIGNLTTVIKGI+KNGGRKFAF GVGP+GC P VKA +LQ
Sbjct: 181 PFLTNSTLFQSHSPQQYVDLVIGNLTTVIKGIYKNGGRKFAFFGVGPLGCFPLVKAVILQ 240

Query: 241 GKDECLEEITQLVKLHNNHLSKTLLQLGKKLEGFVYSYADFYATAIEVTNNPAKYDFKEG 300
           GKDEC +EIT+L KLHN HL KTLL+LGKKLEGFVY+Y D +   IE+ NNPAKY  KEG
Sbjct: 241 GKDECFDEITELAKLHNKHLYKTLLRLGKKLEGFVYTYVDSFTVVIELLNNPAKYGLKEG 300

Query: 301 KVACCGSGPFRGYSSCGGRNGQEYELCGNPSEYLFFDGSHPSEKANQLFAQFLWNGSSEI 360
           KVACCGSGPFRGY SCGGRNG+EY+LC NPS++LFFD +H ++KANQL+A+ LWNG+ + 
Sbjct: 301 KVACCGSGPFRGYFSCGGRNGEEYKLCNNPSQHLFFDATHFTDKANQLYAELLWNGNLQT 360

Query: 361 IRPYNLNVLFHL 368
           I+PYNL  LFH+
Sbjct: 361 IKPYNLKTLFHV 370

BLAST of MS016166 vs. ExPASy TrEMBL
Match: A0A1S3CCQ0 (GDSL esterase/lipase 2-like OS=Cucumis melo OX=3656 GN=LOC103499189 PE=3 SV=1)

HSP 1 Score: 556.6 bits (1433), Expect = 7.7e-155
Identity = 273/372 (73.39%), Postives = 307/372 (82.53%), Query Frame = 0

Query: 1   MASSWLSSSFLVFFIASFLSLQCCRCDDLKPPPK-HVAFFIFGDSLFDVGNNNYINTTTS 60
           MA+S   SS LV+FI   + +Q C+ +   P PK HV  FIFGDSLFD GNNNYIN  T 
Sbjct: 1   MAASKFPSSLLVYFIVIAVCMQICKGNPKIPLPKLHVPLFIFGDSLFDAGNNNYIN--TP 60

Query: 61  FQANFLPYGETFFKFPTGRFSDGRLIPDFIAKYGNLPLIHPYLNPKNKFYLHGVNFASAG 120
           FQ+NF PYGETFF FPTGRFSDGRLIPDFIAKY NLP IHPYLNP NK YLHGVNFASAG
Sbjct: 61  FQSNFWPYGETFFNFPTGRFSDGRLIPDFIAKYANLPFIHPYLNPTNKNYLHGVNFASAG 120

Query: 121 AGALVDTHQGFVIDLKTQLSYFNKVAKVLKEELGHAE--AKALLSRAVYFISIGSNDY-- 180
           AGALV+T QGFVIDLKTQLSYFNKV KV +E  G  E  AKALLSRAVY I+IGSNDY  
Sbjct: 121 AGALVETQQGFVIDLKTQLSYFNKVRKVFEEVGGGHEGGAKALLSRAVYLINIGSNDYLA 180

Query: 181 PFTTNSSLFRSHSPQQYVDLVIGNLTTVIKGIHKNGGRKFAFLGVGPIGCVPTVKATLLQ 240
           PF TNS+LF+SHSPQQYVDLVIGNLTTVIKGI+KNGGRKFAF GVGP+GC P VKA +LQ
Sbjct: 181 PFLTNSTLFQSHSPQQYVDLVIGNLTTVIKGIYKNGGRKFAFFGVGPLGCFPLVKAVILQ 240

Query: 241 GKDECLEEITQLVKLHNNHLSKTLLQLGKKLEGFVYSYADFYATAIEVTNNPAKYDFKEG 300
           GKDEC +EIT+L KLHN HL KTLL+LGKKLEGFVY+Y D +   IE+ NNPAKY  KEG
Sbjct: 241 GKDECFDEITELAKLHNKHLYKTLLRLGKKLEGFVYTYVDSFTVVIELLNNPAKYGLKEG 300

Query: 301 KVACCGSGPFRGYSSCGGRNGQEYELCGNPSEYLFFDGSHPSEKANQLFAQFLWNGSSEI 360
           KVACCGSGPFRGY SCGGRNG+EY+LC NPS++LFFD +H ++KANQL+A+ LWNG+ + 
Sbjct: 301 KVACCGSGPFRGYFSCGGRNGEEYKLCNNPSQHLFFDATHFTDKANQLYAELLWNGNLQT 360

Query: 361 IRPYNLNVLFHL 368
           I+PYNL  LFH+
Sbjct: 361 IKPYNLKTLFHV 370

BLAST of MS016166 vs. ExPASy TrEMBL
Match: A0A6J1GVI4 (GDSL esterase/lipase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111457922 PE=3 SV=1)

HSP 1 Score: 555.8 bits (1431), Expect = 1.3e-154
Identity = 275/370 (74.32%), Postives = 314/370 (84.86%), Query Frame = 0

Query: 1   MASSWLSSSFLVFFIASFLSLQCCRCDDLKPPPKHVAFFIFGDSLFDVGNNNYINTTTSF 60
           MASS   SSFLV+FI S LS+  C     K  P   A FIFGDSLFD GNNNYIN  TSF
Sbjct: 1   MASSCFPSSFLVYFILSLLSIHNC-----KGHPNSAALFIFGDSLFDAGNNNYIN--TSF 60

Query: 61  QANFLPYGETFFKFPTGRFSDGRLIPDFIAKYGNLPLIHPYLNPKNKFYLHGVNFASAGA 120
           +ANF+PYG++FFKFPTGRFSDGRLIPDFIAKY NLPLI PYL+P NK Y+HGVNFASAGA
Sbjct: 61  KANFVPYGQSFFKFPTGRFSDGRLIPDFIAKYANLPLILPYLHPMNKNYVHGVNFASAGA 120

Query: 121 GALVDTHQGFVIDLKTQLSYFNKVAKVLKEELGH-AEAKALLSRAVYFISIGSNDY--PF 180
           GAL +THQG VIDLKTQLSYFNKV KV+ E +GH AEAKALLS+AVYFISIGSNDY  PF
Sbjct: 121 GALAETHQGLVIDLKTQLSYFNKVTKVV-EGIGHPAEAKALLSKAVYFISIGSNDYIAPF 180

Query: 181 TTNSSLFRSHSPQQYVDLVIGNLTTVIKGIHKNGGRKFAFLGVGPIGCVPTVKATLLQGK 240
           TTNS+LF+SHSPQ+YV+LVIGNLTTVIKGIHKNGGRKFAFLGVG IGCVP VKA LLQGK
Sbjct: 181 TTNSTLFQSHSPQEYVELVIGNLTTVIKGIHKNGGRKFAFLGVGDIGCVPLVKAVLLQGK 240

Query: 241 DECLEEITQLVKLHNNHLSKTLLQLGKKLEGFVYSYADFYATAIEVTNNPAKYDFKEGKV 300
           DEC E ITQLV LHN HL KTL  L K+LEGFVYSYAD Y++A +++NNP+KY FKEGK 
Sbjct: 241 DECSENITQLVTLHNKHLYKTLQHLEKELEGFVYSYADLYSSANQISNNPSKYGFKEGKA 300

Query: 301 ACCGSGPFRGYSSCGGRNGQEYELCGNPSEYLFFDGSHPSEKANQLFAQFLWNGSSEIIR 360
           ACCGSGPFRG+ SCGGR+G+EYELC NP+EYLF+D +H ++KA+QL A+++WNGSS+ I+
Sbjct: 301 ACCGSGPFRGFFSCGGRDGEEYELCSNPNEYLFYDSAHLTQKASQLVAEWVWNGSSDSIK 360

Query: 361 PYNLNVLFHL 368
           PYNL  LF++
Sbjct: 361 PYNLKALFNV 362

BLAST of MS016166 vs. TAIR 10
Match: AT5G40990.1 (GDSL lipase 1 )

HSP 1 Score: 364.4 bits (934), Expect = 1.1e-100
Identity = 189/372 (50.81%), Postives = 244/372 (65.59%), Query Frame = 0

Query: 3   SSWLSSSFLVF-FIASFLSLQCCRCDDLKPPPKHVAFFIFGDSLFDVGNNNYINTTTSFQ 62
           S  +S +FL +  I S  S+ C   D+        A F+FGDS+FD GNNNYI+T +S +
Sbjct: 4   SQLVSITFLAYTIIISIGSINC--IDNNNLVTNQSALFVFGDSVFDAGNNNYIDTLSSVR 63

Query: 63  ANFLPYGETFFKFPTGRFSDGRLIPDFIAKYGNLPLIHPYLNP--KNKFYLHGVNFASAG 122
           +N+ PYG+T FK PTGR SDGRLIPDFIA+Y  LPLI P L P   N  + +GVNFAS G
Sbjct: 64  SNYWPYGQTTFKSPTGRVSDGRLIPDFIAEYAWLPLIPPNLQPFNGNSQFAYGVNFASGG 123

Query: 123 AGALVDTHQGFVIDLKTQLSYFNKVAKVLKEELGHAEAKALLSRAVYFISIGSND--YPF 182
           AGALV T  G VI+L+TQL+ F KV ++L+ +LG AE K ++SRAVY   IG ND  YPF
Sbjct: 124 AGALVGTFSGLVINLRTQLNNFKKVEEMLRSKLGDAEGKRVISRAVYLFHIGLNDYQYPF 183

Query: 183 TTNSSLFRSHSPQQYVDLVIGNLTTVIKGIHKNGGRKFAFLGVGPIGCVPTVKATLLQGK 242
           TTNSSLF+S S ++YVD V+GN+T V K ++  GGRKF  L  GP  C P          
Sbjct: 184 TTNSSLFQSISNEKYVDYVVGNMTDVFKEVYNLGGRKFGILNTGPYDCAPASLVIDQTKI 243

Query: 243 DECLEEITQLVKLHNNHLSKTLLQLGKKLEGFVYSYADFYATAIEVTNNPAKYDFKEGKV 302
             C + +T+L+ +HN  L   L +L  +L GF Y+  D++ +  E  N+P+KY FKEGK 
Sbjct: 244 RSCFQPVTELINMHNEKLLNGLRRLNHELSGFKYALHDYHTSLSERMNDPSKYGFKEGKK 303

Query: 303 ACCGSGPFRGYSSCGGRNG--QEYELCGNPSEYLFFDGSHPSEKANQLFAQFLWNGSSEI 362
           ACCGSGP RG ++CGGR G  Q YELC N ++YLFFD  H +EKAN+  A+ +W+G + I
Sbjct: 304 ACCGSGPLRGINTCGGRMGLSQSYELCENVTDYLFFDPFHLTEKANRQIAELIWSGPTNI 363

Query: 363 IRPYNLNVLFHL 368
             PYNL  LF L
Sbjct: 364 TGPYNLKALFEL 373

BLAST of MS016166 vs. TAIR 10
Match: AT1G53940.1 (GDSL-motif lipase 2 )

HSP 1 Score: 348.2 bits (892), Expect = 8.0e-96
Identity = 177/360 (49.17%), Postives = 237/360 (65.83%), Query Frame = 0

Query: 8   SSFLVFFIASFL----SLQCCRCDDLKPPPKHVAFFIFGDSLFDVGNNNYINTTTSFQAN 67
           S+ ++FF  + +    S+ C   ++        A F+FGDS+FD GNNNYI+T  SF++N
Sbjct: 6   STLIIFFAYTTIILIGSINCRDNNNNNLVTNQSALFVFGDSVFDAGNNNYIDTLPSFRSN 65

Query: 68  FLPYGETFFKFPTGRFSDGRLIPDFIAKYGNLPLIHPYLNPKN--KFYLHGVNFASAGAG 127
           + PYG+T FKFPTGR SDGR IPDFIA+Y  LPLI  YL P N    + +GV+FASAGAG
Sbjct: 66  YWPYGQTTFKFPTGRVSDGRTIPDFIAEYAWLPLIPAYLQPSNGKNQFPYGVSFASAGAG 125

Query: 128 ALVDTHQGFVIDLKTQLSYFNKVAKVLKEELGHAEAKALLSRAVYFISIGSND--YPFTT 187
           ALV T  G VI+LK+QL+ F KV K+L+  LG A+ K ++SRAVY   IG ND  YPF+T
Sbjct: 126 ALVGTFPGMVINLKSQLNNFKKVEKLLRSTLGEAQGKMVISRAVYLFHIGVNDYQYPFST 185

Query: 188 NSSLFRSHSPQQYVDLVIGNLTTVIKGIHKNGGRKFAFLGVGPIGCVPTVKATLLQGKDE 247
           NSS+F+S   + YVD V+GN T VIK ++K GGRKF FL +G   C P            
Sbjct: 186 NSSIFQSSPQEIYVDFVVGNTTAVIKEVYKIGGRKFGFLNMGAYDCAPASLIIDQTKIGT 245

Query: 248 CLEEITQLVKLHNNHLSKTLLQLGKKLEGFVYSYADFYATAIEVTNNPAKYDFKEGKVAC 307
           C + +T+L+ LHN  L   L +L ++L GF Y+  D++ +     NNP+KY FKEGK+AC
Sbjct: 246 CFKPVTELINLHNEKLESGLRRLERELSGFKYALHDYHTSLSVRMNNPSKYGFKEGKMAC 305

Query: 308 CGSGPFRGYSSCGGRNG--QEYELCGNPSEYLFFDGSHPSEKANQLFAQFLWNGSSEIIR 358
           CG+GP RG ++CGGR G  Q YELC   ++YLFFD  H +EKA+Q  A+ +W+G +  +R
Sbjct: 306 CGTGPLRGINTCGGRMGVSQSYELCEKVTDYLFFDHFHLTEKAHQQIAELIWSGPTNGLR 365

BLAST of MS016166 vs. TAIR 10
Match: AT1G53920.1 (GDSL-motif lipase 5 )

HSP 1 Score: 344.0 bits (881), Expect = 1.5e-94
Identity = 184/371 (49.60%), Postives = 242/371 (65.23%), Query Frame = 0

Query: 8   SSFLVFFIAS---FLSLQCCRCDDLKPPPKHVAFFIFGDSLFDVGNNNYINTTTSFQANF 67
           SSF+ F ++S   FL+ +              A F+FGDS  D GNNNYINTTT  QANF
Sbjct: 16  SSFIFFIVSSTILFLAGKSSAKISHNGDNNVTALFLFGDSFLDAGNNNYINTTTLDQANF 75

Query: 68  LPYGETFFKFPTGRFSDGRLIPDFIAKYGNLPLIHPYLNPKN-KFYLHGVNFASAGAGAL 127
            PYG+TFF  PTGRFSDGRLI DFIA+Y NLPLI P+L P N +  L+GVNFASAGAGAL
Sbjct: 76  PPYGQTFFGLPTGRFSDGRLISDFIAEYANLPLIPPFLEPGNSQKKLYGVNFASAGAGAL 135

Query: 128 VDTHQGFVIDLKTQLSYFNKVAKVLKEELGHAEAKALLSRAVYFISIGSNDYP--FTTNS 187
           V+T QG VI+L+TQL ++ KV ++ +   G  E+K  +SRAVY ISIGSNDY   F TN 
Sbjct: 136 VETFQGSVINLRTQLDHYKKVERLWRTNFGKEESKKRISRAVYLISIGSNDYSSIFLTNQ 195

Query: 188 SLFRSHSPQQYVDLVIGNLTTVIKGIHKNGGRKFAFLGVGPIGCVPTVKATLLQGKDECL 247
           SL  S S  Q+VD+VIGNLTT I  I+K GGRKF FL V  +GC P ++    +  D CL
Sbjct: 196 SLPISMS--QHVDIVIGNLTTFIHEIYKIGGRKFGFLNVPDLGCFPALRILQPKNDDSCL 255

Query: 248 EEITQLVKLHNNHLSKTLLQLGKKLEGFVYSYADFYATAIEVTNNPAKYDFKEGKVACCG 307
            + ++L  +HN  L+  L Q+ ++++GF +S  D   +      +P+K+ FKEG+ ACCG
Sbjct: 256 RDASRLASMHNRALTNLLFQMQRQVKGFKFSLFDMNKSLRLRMQHPSKFGFKEGEEACCG 315

Query: 308 SGPFRGYSSCGG-RNGQEYELCGNPSEYLFFDGSHPSEKANQLFAQFLWNG----SSEII 367
           +G +RG  SCGG R  +EY+LC NP +Y+F+D  H ++     FA  +WNG     S ++
Sbjct: 316 TGKWRGVFSCGGKRIVKEYQLCENPKDYIFWDSLHLTQNTYNQFANLIWNGGHMSDSLVV 375

BLAST of MS016166 vs. TAIR 10
Match: AT3G14225.1 (GDSL-motif lipase 4 )

HSP 1 Score: 342.4 bits (877), Expect = 4.4e-94
Identity = 177/379 (46.70%), Postives = 240/379 (63.32%), Query Frame = 0

Query: 1   MASSWLSSSFLVFFIASF-LSLQCCRC-DDLKPPPKHVAFFIFGDSLFDVGNNNYINTTT 60
           MAS   +S  ++ FI +  LS+    C +DLK      A F FGDSLF+ GNNNY ++ +
Sbjct: 1   MASPRFNSIIIILFICTISLSIVSISCKEDLK--TNQAALFAFGDSLFEAGNNNYFDSIS 60

Query: 61  SFQANFLPYGETFFKFPTGRFSDGRLIPDFIAKYGNLPLIHPYLNP--KNKFYLHGVNFA 120
           SF++NF PYG+T FKFPTGR SDGR++ DFIA+Y  LPLI P L P   N    +G+NFA
Sbjct: 61  SFRSNFWPYGKTTFKFPTGRVSDGRIMIDFIAEYAWLPLIPPNLQPGYSNSQLTYGLNFA 120

Query: 121 SAGAGALVDTHQGFVI----DLKTQLSYFNKVAKVLKEELGHAEAKALLSRAVYFISIGS 180
           +  AG    T  G V     DL TQL+ F  V K L+  LG AEA+ ++S+AVY   IG+
Sbjct: 121 TTAAGVFAGTFPGSVTNLSKDLGTQLNNFKNVEKTLRSNLGDAEARRVISKAVYLFHIGA 180

Query: 181 ND--YPFTTNSSLFRSHSPQQYVDLVIGNLTTVIKGIHKNGGRKFAFLGVGPIGCVPTVK 240
           ND  YPF  N+S F + + ++++D VIGN TTVI+ ++K G RKF FL +GP GC P+  
Sbjct: 181 NDYQYPFFANTSTFSNTTKERFIDFVIGNTTTVIEELYKLGARKFGFLSLGPFGCTPSAL 240

Query: 241 ATLLQGKDECLEEITQLVKLHNNHLSKTLLQLGKKLEGFVYSYADFYATAIEVTNNPAKY 300
                    C E +T+L+ LHN    K L +L ++L GF Y+  DF+ +  +  NNP++Y
Sbjct: 241 IINSTKIGSCFEPVTELINLHNQEFPKVLRRLERRLSGFKYALHDFHTSLSQRINNPSRY 300

Query: 301 DFKEGKVACCGSGPFRGYSSCGGRNG--QEYELCGNPSEYLFFDGSHPSEKANQLFAQFL 360
            FKEG++ACCGSGP RG ++CG RNG  Q Y+LC N  +Y+FFD SH +E A+Q  A+ +
Sbjct: 301 GFKEGEMACCGSGPLRGINTCGFRNGPSQGYKLCENADDYVFFDPSHLTETAHQQIAELI 360

Query: 361 WNGSSEIIRPYNLNVLFHL 368
           W+G   +  PYNL  LF L
Sbjct: 361 WSGPPNVTAPYNLKTLFRL 377

BLAST of MS016166 vs. TAIR 10
Match: AT1G53990.1 (GDSL-motif lipase 3 )

HSP 1 Score: 336.3 bits (861), Expect = 3.2e-92
Identity = 175/365 (47.95%), Postives = 232/365 (63.56%), Query Frame = 0

Query: 11  LVFFIAS-FLSLQCCRC-DDLKPPPKHVAFFIFGDSLFDVGNNNYINTTTSFQANFLPYG 70
           ++FF+ +  LS+    C D+        A F+FGDSLFD GNNNYINT +SF++N  PYG
Sbjct: 7   IIFFVYTIILSIGSINCIDNNNLVTNQAALFVFGDSLFDAGNNNYINTVSSFRSNIWPYG 66

Query: 71  ETFFKFPTGRFSDGRLIPDFIAKYGNLPLIHPYLNPK--NKFYLHGVNFASAGAGALVDT 130
           +T FKFPTGR SDG        +   LP I P L P   N  + +GV+FASAGAGAL ++
Sbjct: 67  QTNFKFPTGRLSDG-------PEKAWLPSIPPNLQPNNGNNQFTYGVSFASAGAGALAES 126

Query: 131 HQGFVIDLKTQLSYFNKVAKVLKEELGHAEAKALLSRAVYFISIGSND--YPFTTNSSLF 190
             G VI+L TQL+ F  V K L+ ELG AE K + SRAVY   IG+ND  YPF+ NSS F
Sbjct: 127 FLGMVINLGTQLNNFKDVEKSLRSELGDAETKRVFSRAVYLFHIGANDYFYPFSANSSTF 186

Query: 191 RSHSPQQYVDLVIGNLTTVIKGIHKNGGRKFAFLGVGPIGCVPTVKATLLQGKDECLEEI 250
           +S+S +++VD VIGN+T VI+ ++K GGRKF FL VGP  C P            C + +
Sbjct: 187 KSNSKEKFVDFVIGNITFVIEEVYKMGGRKFGFLNVGPYECSPNSLIRDRTKIGSCFKPV 246

Query: 251 TQLVKLHNNHLSKTLLQLGKKLEGFVYSYADFYATAIEVTNNPAKYDFKEGKVACCGSGP 310
            +L+ +HN      L +L ++L GF Y+  D++ +  E  N+P+KY FKEGK ACCGSGP
Sbjct: 247 AELIDMHNKKFPDVLRRLQRQLSGFRYALHDYHTSLSERINSPSKYGFKEGKKACCGSGP 306

Query: 311 FRGYSSCGGRNG--QEYELCGNPSEYLFFDGSHPSEKANQLFAQFLWNGSSEIIRPYNLN 368
            RG ++CG R G  Q Y LC N ++YLF+D SH +EKA++  A+ +WNG   + RPYNL 
Sbjct: 307 LRGINTCGNRIGPSQGYGLCENVTDYLFYDSSHLTEKAHRQIAELIWNGPPNVTRPYNLK 364