MS016161 (gene) Bitter gourd (TR) v1

Overview
NameMS016161
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
Descriptionnucleolin 1
Locationscaffold9_2: 788514 .. 789621 (+)
RNA-Seq ExpressionMS016161
SyntenyMS016161
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGATAGCCGGCGAAGACGGTTCAAAGTTAGTTGTAGGTGAGCCAGTGACGATATTCGGGAGAGGCTCAGGATTTGCTTCCAAAGATCGGACAGTATCTCGTCGTCACCTTTTAATCGAAGCTAAAACCTCAGAGAACTGTGATGGAGCTCCCGTGGAGCCTTGGATTTCCTTCGAGGTTATTGGGAGAAACCCTATTTGGGTTCGGAGCGGCAAAAATGGCGAAATCAGAACATTTAGAAGGTCGGAAAAGGGCGAGATTGCGCCTGGTGAATCGTTCTGCGTGGGCGGGCAGGAACCGACTTGGTTCAAATTAAACAAAATCGCTGAAATCGAAGAAGGGAAGCGAATTGTTCCGAAGACCGAGGATCAATTTGCTGAGAGGTTGACGCGTTCTTCTGATTCGGAGGATATCGATGTTTCTGGCATTGATCCTGTCAAAGGTTTGCTCCCGAAAACCTATCTTGATTTTCCGAGAAAAATTGAAGGAAAATAAACTAACCATCACTTTTCAGATTGTTTACAAATTAAGCGTTGGATTTTGATAATGAAAAGTAAAATTAGCAGTTACTAAAAAGAACAAACGAGAAAATTGTTTAGAAGTTCCAAATTGCAAATCTATCATACTGATTGTGCTTACTGTTCCCTTGTCAGAATTTGGTTTTCTTGTTATGGGGCACGAGTTTGAATGCTATGGCAATCGAGTGATTCGTGATGCAAAAAAGTGGGACTGGTTTCTGGATGAGCCGAGAAAAGGAAGCGATGATGATGAAGATTATGGGAGGAGAAAGAGGAGAGGAGTAAGGGGAAAGAGAAAAAAAGGAGCAAACAGTGACGATGAAGATTGGACAGATGAGAATGAAGATGCTGTGGAAATGATTGCAAAAGCAAAAAAGGTTCAAAGACCCAAATATTCCACGAGATCAAAGGATCACAATAAAACTAACAAGGATCAAAATAAAGCTGCTAATTCTAACAAAGACTATGATGAAGAAGATGAAGATGATGAAACTTTAGGAGGTTTTATTGTAGATGATGAAGTAGACAACGAAGAACAGATGGACGAAGATGAAGAAGAAGAATTTTCGGACGACGATGATGAGGAT

mRNA sequence

ATGGAGATAGCCGGCGAAGACGGTTCAAAGTTAGTTGTAGGTGAGCCAGTGACGATATTCGGGAGAGGCTCAGGATTTGCTTCCAAAGATCGGACAGTATCTCGTCGTCACCTTTTAATCGAAGCTAAAACCTCAGAGAACTGTGATGGAGCTCCCGTGGAGCCTTGGATTTCCTTCGAGGTTATTGGGAGAAACCCTATTTGGGTTCGGAGCGGCAAAAATGGCGAAATCAGAACATTTAGAAGGTCGGAAAAGGGCGAGATTGCGCCTGGTGAATCGTTCTGCGTGGGCGGGCAGGAACCGACTTGGTTCAAATTAAACAAAATCGCTGAAATCGAAGAAGGGAAGCGAATTGTTCCGAAGACCGAGGATCAATTTGCTGAGAGGTTGACGCGTTCTTCTGATTCGGAGGATATCGATGTTTCTGGCATTGATCCTGTCAAAGAATTTGGTTTTCTTGTTATGGGGCACGAGTTTGAATGCTATGGCAATCGAGTGATTCGTGATGCAAAAAAGTGGGACTGGTTTCTGGATGAGCCGAGAAAAGGAAGCGATGATGATGAAGATTATGGGAGGAGAAAGAGGAGAGGAGTAAGGGGAAAGAGAAAAAAAGGAGCAAACAGTGACGATGAAGATTGGACAGATGAGAATGAAGATGCTGTGGAAATGATTGCAAAAGCAAAAAAGGTTCAAAGACCCAAATATTCCACGAGATCAAAGGATCACAATAAAACTAACAAGGATCAAAATAAAGCTGCTAATTCTAACAAAGACTATGATGAAGAAGATGAAGATGATGAAACTTTAGGAGGTTTTATTGTAGATGATGAAGTAGACAACGAAGAACAGATGGACGAAGATGAAGAAGAAGAATTTTCGGACGACGATGATGAGGAT

Coding sequence (CDS)

ATGGAGATAGCCGGCGAAGACGGTTCAAAGTTAGTTGTAGGTGAGCCAGTGACGATATTCGGGAGAGGCTCAGGATTTGCTTCCAAAGATCGGACAGTATCTCGTCGTCACCTTTTAATCGAAGCTAAAACCTCAGAGAACTGTGATGGAGCTCCCGTGGAGCCTTGGATTTCCTTCGAGGTTATTGGGAGAAACCCTATTTGGGTTCGGAGCGGCAAAAATGGCGAAATCAGAACATTTAGAAGGTCGGAAAAGGGCGAGATTGCGCCTGGTGAATCGTTCTGCGTGGGCGGGCAGGAACCGACTTGGTTCAAATTAAACAAAATCGCTGAAATCGAAGAAGGGAAGCGAATTGTTCCGAAGACCGAGGATCAATTTGCTGAGAGGTTGACGCGTTCTTCTGATTCGGAGGATATCGATGTTTCTGGCATTGATCCTGTCAAAGAATTTGGTTTTCTTGTTATGGGGCACGAGTTTGAATGCTATGGCAATCGAGTGATTCGTGATGCAAAAAAGTGGGACTGGTTTCTGGATGAGCCGAGAAAAGGAAGCGATGATGATGAAGATTATGGGAGGAGAAAGAGGAGAGGAGTAAGGGGAAAGAGAAAAAAAGGAGCAAACAGTGACGATGAAGATTGGACAGATGAGAATGAAGATGCTGTGGAAATGATTGCAAAAGCAAAAAAGGTTCAAAGACCCAAATATTCCACGAGATCAAAGGATCACAATAAAACTAACAAGGATCAAAATAAAGCTGCTAATTCTAACAAAGACTATGATGAAGAAGATGAAGATGATGAAACTTTAGGAGGTTTTATTGTAGATGATGAAGTAGACAACGAAGAACAGATGGACGAAGATGAAGAAGAAGAATTTTCGGACGACGATGATGAGGAT

Protein sequence

MEIAGEDGSKLVVGEPVTIFGRGSGFASKDRTVSRRHLLIEAKTSENCDGAPVEPWISFEVIGRNPIWVRSGKNGEIRTFRRSEKGEIAPGESFCVGGQEPTWFKLNKIAEIEEGKRIVPKTEDQFAERLTRSSDSEDIDVSGIDPVKEFGFLVMGHEFECYGNRVIRDAKKWDWFLDEPRKGSDDDEDYGRRKRRGVRGKRKKGANSDDEDWTDENEDAVEMIAKAKKVQRPKYSTRSKDHNKTNKDQNKAANSNKDYDEEDEDDETLGGFIVDDEVDNEEQMDEDEEEEFSDDDDED
Homology
BLAST of MS016161 vs. NCBI nr
Match: XP_022152655.1 (protein bfr2 [Momordica charantia])

HSP 1 Score: 587.0 bits (1512), Expect = 9.0e-164
Identity = 299/299 (100.00%), Postives = 299/299 (100.00%), Query Frame = 0

Query: 1   MEIAGEDGSKLVVGEPVTIFGRGSGFASKDRTVSRRHLLIEAKTSENCDGAPVEPWISFE 60
           MEIAGEDGSKLVVGEPVTIFGRGSGFASKDRTVSRRHLLIEAKTSENCDGAPVEPWISFE
Sbjct: 1   MEIAGEDGSKLVVGEPVTIFGRGSGFASKDRTVSRRHLLIEAKTSENCDGAPVEPWISFE 60

Query: 61  VIGRNPIWVRSGKNGEIRTFRRSEKGEIAPGESFCVGGQEPTWFKLNKIAEIEEGKRIVP 120
           VIGRNPIWVRSGKNGEIRTFRRSEKGEIAPGESFCVGGQEPTWFKLNKIAEIEEGKRIVP
Sbjct: 61  VIGRNPIWVRSGKNGEIRTFRRSEKGEIAPGESFCVGGQEPTWFKLNKIAEIEEGKRIVP 120

Query: 121 KTEDQFAERLTRSSDSEDIDVSGIDPVKEFGFLVMGHEFECYGNRVIRDAKKWDWFLDEP 180
           KTEDQFAERLTRSSDSEDIDVSGIDPVKEFGFLVMGHEFECYGNRVIRDAKKWDWFLDEP
Sbjct: 121 KTEDQFAERLTRSSDSEDIDVSGIDPVKEFGFLVMGHEFECYGNRVIRDAKKWDWFLDEP 180

Query: 181 RKGSDDDEDYGRRKRRGVRGKRKKGANSDDEDWTDENEDAVEMIAKAKKVQRPKYSTRSK 240
           RKGSDDDEDYGRRKRRGVRGKRKKGANSDDEDWTDENEDAVEMIAKAKKVQRPKYSTRSK
Sbjct: 181 RKGSDDDEDYGRRKRRGVRGKRKKGANSDDEDWTDENEDAVEMIAKAKKVQRPKYSTRSK 240

Query: 241 DHNKTNKDQNKAANSNKDYDEEDEDDETLGGFIVDDEVDNEEQMDEDEEEEFSDDDDED 300
           DHNKTNKDQNKAANSNKDYDEEDEDDETLGGFIVDDEVDNEEQMDEDEEEEFSDDDDED
Sbjct: 241 DHNKTNKDQNKAANSNKDYDEEDEDDETLGGFIVDDEVDNEEQMDEDEEEEFSDDDDED 299

BLAST of MS016161 vs. NCBI nr
Match: XP_038898830.1 (protein PIF [Benincasa hispida])

HSP 1 Score: 464.5 bits (1194), Expect = 6.7e-127
Identity = 248/307 (80.78%), Postives = 263/307 (85.67%), Query Frame = 0

Query: 1   MEIAGEDGSKLVVGEPVTIFGRGSGFASKDRTVSRRHLLIEAKTSENCDGAPVEPWISFE 60
           MEIAGEDGSKLV+ EPVTIFGRGSGFASKDRTVSRRH+LIEAKTSEN +GAP+EP +SFE
Sbjct: 1   MEIAGEDGSKLVLREPVTIFGRGSGFASKDRTVSRRHILIEAKTSENSNGAPIEPTVSFE 60

Query: 61  VIGRNPIWVRSGKNGEIRTFRRSEKGEIAPGESFCVGGQEPTWFKLNKIAEIEEGKRIVP 120
           VIGRNPIWVRS KNGEIRTFRRSEKGE+A GESFCVG QEP WF+LNKIAE EE K+   
Sbjct: 61  VIGRNPIWVRSSKNGEIRTFRRSEKGEMALGESFCVGSQEPIWFELNKIAEFEERKQF-- 120

Query: 121 KTEDQFAERLTRSSDSEDIDVSGIDPVKEFGFLVMGHEFECYGNRVIRDAKKWDWFLDEP 180
                     +RSSDS DIDVSGIDPVKEFGFL MGHEFECYGNRVIRD KKWDWFLDEP
Sbjct: 121 ---------FSRSSDSVDIDVSGIDPVKEFGFLAMGHEFECYGNRVIRDPKKWDWFLDEP 180

Query: 181 RKGSDDDEDYGRRKRRGVRGKRKKGANSDDEDWTDENEDAVEMIAKAKKVQRPKYSTRSK 240
           RK SDDDEDYGR+K+RGV+GKRKKGANSDDEDWTDENEDAVEMI K KK +RPKYSTRSK
Sbjct: 181 RKDSDDDEDYGRKKKRGVKGKRKKGANSDDEDWTDENEDAVEMITKTKKFRRPKYSTRSK 240

Query: 241 DHNKTNKDQNKAANSNKDYD---EEDEDDETLGGFIVDDEVDNEEQMDED-----EEEEF 300
           D +KT  DQ KAA SNKDYD   EEDEDDETLGGFIVDDE DN EQMDED     EEEEF
Sbjct: 241 DRHKTPNDQKKAARSNKDYDEEVEEDEDDETLGGFIVDDEEDNVEQMDEDEDEDEEEEEF 296

BLAST of MS016161 vs. NCBI nr
Match: XP_008447630.1 (PREDICTED: nucleolin 1 [Cucumis melo] >TYK03672.1 nucleolin 1 [Cucumis melo var. makuwa])

HSP 1 Score: 461.8 bits (1187), Expect = 4.4e-126
Identity = 243/303 (80.20%), Postives = 264/303 (87.13%), Query Frame = 0

Query: 1   MEIAGEDGSKLVVGEPVTIFGRGSGFASKDRTVSRRHLLIEAKTSENCDGAPVEPWISFE 60
           MEIAGEDGSKLV+ EPVTIFGRGSGFASKDRT+SRRH+LIEAKTS+N +GAP+EP +SFE
Sbjct: 1   MEIAGEDGSKLVLREPVTIFGRGSGFASKDRTISRRHILIEAKTSDNTNGAPMEPKVSFE 60

Query: 61  VIGRNPIWVRSGKNGEIRTFRRSEKGEIAPGESFCVGGQEPTWFKLNKIAEIEEGKRIVP 120
           VIGRNPIWVRS KNGEIRTFRRSEKG++APGESFCVGGQEP WF+LNKI E EEGK +  
Sbjct: 61  VIGRNPIWVRSSKNGEIRTFRRSEKGKMAPGESFCVGGQEPIWFELNKITEFEEGKLV-- 120

Query: 121 KTEDQFAERLTRSSDSEDIDVSGIDPVKEFGFLVMGHEFECYGNRVIRDAKKWDWFLDEP 180
                     +RSSDS+DIDVSGIDPVKEFGFLVMGHEFECYGNRVIRD KKWDWFLD P
Sbjct: 121 ---------SSRSSDSDDIDVSGIDPVKEFGFLVMGHEFECYGNRVIRDVKKWDWFLDGP 180

Query: 181 RKGS-DDDEDYGRRKRRGVRGKRKKGANSDDEDWTDENEDAVEMIAKAKKVQRPKYSTRS 240
            K S DDDEDY R+K+RGVRGKRKKGANSDDEDWTDENEDAV+MI K +K QRPKYSTRS
Sbjct: 181 SKDSHDDDEDYERKKKRGVRGKRKKGANSDDEDWTDENEDAVQMITKVQKSQRPKYSTRS 240

Query: 241 KDHNKTNKDQNKAANSNKDYD---EEDEDDETLGGFIVDDEVDNEEQMDEDEEEEFSDDD 300
           KD NK + DQ KAA SNKDYD   EEDEDDETLGGFIVDDEVDN EQMDEDEEEEF DD+
Sbjct: 241 KDRNKISNDQRKAAKSNKDYDGEVEEDEDDETLGGFIVDDEVDNVEQMDEDEEEEFLDDE 292

BLAST of MS016161 vs. NCBI nr
Match: KAA0036698.1 (nucleolin 1 [Cucumis melo var. makuwa])

HSP 1 Score: 461.5 bits (1186), Expect = 5.7e-126
Identity = 242/303 (79.87%), Postives = 264/303 (87.13%), Query Frame = 0

Query: 1   MEIAGEDGSKLVVGEPVTIFGRGSGFASKDRTVSRRHLLIEAKTSENCDGAPVEPWISFE 60
           MEIAGEDGSKL++ EPVTIFGRGSGFASKDRT+SRRH+LIEAKTS+N +GAP+EP +SFE
Sbjct: 1   MEIAGEDGSKLILREPVTIFGRGSGFASKDRTISRRHILIEAKTSDNTNGAPMEPKVSFE 60

Query: 61  VIGRNPIWVRSGKNGEIRTFRRSEKGEIAPGESFCVGGQEPTWFKLNKIAEIEEGKRIVP 120
           VIGRNPIWVRS KNGEIRTFRRSEKG++APGESFCVGGQEP WF+LNKI E EEGK +  
Sbjct: 61  VIGRNPIWVRSSKNGEIRTFRRSEKGKMAPGESFCVGGQEPIWFELNKITEFEEGKLV-- 120

Query: 121 KTEDQFAERLTRSSDSEDIDVSGIDPVKEFGFLVMGHEFECYGNRVIRDAKKWDWFLDEP 180
                     +RSSDS+DIDVSGIDPVKEFGFLVMGHEFECYGNRVIRD KKWDWFLD P
Sbjct: 121 ---------SSRSSDSDDIDVSGIDPVKEFGFLVMGHEFECYGNRVIRDVKKWDWFLDGP 180

Query: 181 RKGS-DDDEDYGRRKRRGVRGKRKKGANSDDEDWTDENEDAVEMIAKAKKVQRPKYSTRS 240
            K S DDDEDY R+K+RGVRGKRKKGANSDDEDWTDENEDAV+MI K +K QRPKYSTRS
Sbjct: 181 SKDSHDDDEDYERKKKRGVRGKRKKGANSDDEDWTDENEDAVQMITKVQKSQRPKYSTRS 240

Query: 241 KDHNKTNKDQNKAANSNKDYD---EEDEDDETLGGFIVDDEVDNEEQMDEDEEEEFSDDD 300
           KD NK + DQ KAA SNKDYD   EEDEDDETLGGFIVDDEVDN EQMDEDEEEEF DD+
Sbjct: 241 KDRNKISNDQRKAAKSNKDYDGEVEEDEDDETLGGFIVDDEVDNVEQMDEDEEEEFLDDE 292

BLAST of MS016161 vs. NCBI nr
Match: XP_022980683.1 (protein BFR2 [Cucurbita maxima])

HSP 1 Score: 458.4 bits (1178), Expect = 4.8e-125
Identity = 240/300 (80.00%), Postives = 262/300 (87.33%), Query Frame = 0

Query: 1   MEIAGEDGSKLVVGEPVTIFGRGSGFASKDRTVSRRHLLIEAKTSENCDGAPVEPWISFE 60
           MEIAGEDGSKLV+ EPVTIFGRGSGFA KDRTVSR H+LI+AKTSEN +G P+EP +SFE
Sbjct: 1   MEIAGEDGSKLVLREPVTIFGRGSGFACKDRTVSRCHILIKAKTSENSNGVPMEPRVSFE 60

Query: 61  VIGRNPIWVRSGKNGEIRTFRRSEKGEIAPGESFCVGGQEPTWFKLNKIAEIEEGKRIVP 120
           VIG+NPIWVRSGKNGEIRTFR SEKGE+ PGESFC+GGQEP WF+LNKIAE EEG++I  
Sbjct: 61  VIGKNPIWVRSGKNGEIRTFRSSEKGEMVPGESFCMGGQEPMWFQLNKIAEFEEGEKI-- 120

Query: 121 KTEDQFAERLTRSSDSEDIDVSGIDPVKEFGFLVMGHEFECYGNRVIRDAKKWDWFLDEP 180
                     +RSSDS+DIDVSGIDPVKEFGFL  GHEFECYGNRVIRDAKKWDWFLDE 
Sbjct: 121 ---------CSRSSDSDDIDVSGIDPVKEFGFLAKGHEFECYGNRVIRDAKKWDWFLDER 180

Query: 181 RKGSDDDEDYGRRKRRGVRGKRKKGANSDDEDWTDENEDAVEMIAKAKKVQRPKYSTRSK 240
           RK SDD+EDYGR+++  VRGKRKKGAN+DDEDWTDENEDAVEMI K KK QRPKYSTRSK
Sbjct: 181 RKDSDDEEDYGRKQKSRVRGKRKKGANNDDEDWTDENEDAVEMITKTKKFQRPKYSTRSK 240

Query: 241 DHNKTNKDQNKAANSNKDYDEEDEDDETLGGFIVDDEVDNEEQMDEDE-EEEFSDDDDED 300
           D NKT+KDQ KAA SNKDY EEDEDDETLGGFIVDDEVDNEE MDEDE EEEFSDD ++D
Sbjct: 241 DRNKTHKDQKKAAKSNKDYVEEDEDDETLGGFIVDDEVDNEELMDEDEDEEEFSDDKEDD 289

BLAST of MS016161 vs. ExPASy TrEMBL
Match: A0A6J1DEJ6 (protein bfr2 OS=Momordica charantia OX=3673 GN=LOC111020320 PE=4 SV=1)

HSP 1 Score: 587.0 bits (1512), Expect = 4.4e-164
Identity = 299/299 (100.00%), Postives = 299/299 (100.00%), Query Frame = 0

Query: 1   MEIAGEDGSKLVVGEPVTIFGRGSGFASKDRTVSRRHLLIEAKTSENCDGAPVEPWISFE 60
           MEIAGEDGSKLVVGEPVTIFGRGSGFASKDRTVSRRHLLIEAKTSENCDGAPVEPWISFE
Sbjct: 1   MEIAGEDGSKLVVGEPVTIFGRGSGFASKDRTVSRRHLLIEAKTSENCDGAPVEPWISFE 60

Query: 61  VIGRNPIWVRSGKNGEIRTFRRSEKGEIAPGESFCVGGQEPTWFKLNKIAEIEEGKRIVP 120
           VIGRNPIWVRSGKNGEIRTFRRSEKGEIAPGESFCVGGQEPTWFKLNKIAEIEEGKRIVP
Sbjct: 61  VIGRNPIWVRSGKNGEIRTFRRSEKGEIAPGESFCVGGQEPTWFKLNKIAEIEEGKRIVP 120

Query: 121 KTEDQFAERLTRSSDSEDIDVSGIDPVKEFGFLVMGHEFECYGNRVIRDAKKWDWFLDEP 180
           KTEDQFAERLTRSSDSEDIDVSGIDPVKEFGFLVMGHEFECYGNRVIRDAKKWDWFLDEP
Sbjct: 121 KTEDQFAERLTRSSDSEDIDVSGIDPVKEFGFLVMGHEFECYGNRVIRDAKKWDWFLDEP 180

Query: 181 RKGSDDDEDYGRRKRRGVRGKRKKGANSDDEDWTDENEDAVEMIAKAKKVQRPKYSTRSK 240
           RKGSDDDEDYGRRKRRGVRGKRKKGANSDDEDWTDENEDAVEMIAKAKKVQRPKYSTRSK
Sbjct: 181 RKGSDDDEDYGRRKRRGVRGKRKKGANSDDEDWTDENEDAVEMIAKAKKVQRPKYSTRSK 240

Query: 241 DHNKTNKDQNKAANSNKDYDEEDEDDETLGGFIVDDEVDNEEQMDEDEEEEFSDDDDED 300
           DHNKTNKDQNKAANSNKDYDEEDEDDETLGGFIVDDEVDNEEQMDEDEEEEFSDDDDED
Sbjct: 241 DHNKTNKDQNKAANSNKDYDEEDEDDETLGGFIVDDEVDNEEQMDEDEEEEFSDDDDED 299

BLAST of MS016161 vs. ExPASy TrEMBL
Match: A0A5D3BXV5 (Nucleolin 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold863G00290 PE=4 SV=1)

HSP 1 Score: 461.8 bits (1187), Expect = 2.1e-126
Identity = 243/303 (80.20%), Postives = 264/303 (87.13%), Query Frame = 0

Query: 1   MEIAGEDGSKLVVGEPVTIFGRGSGFASKDRTVSRRHLLIEAKTSENCDGAPVEPWISFE 60
           MEIAGEDGSKLV+ EPVTIFGRGSGFASKDRT+SRRH+LIEAKTS+N +GAP+EP +SFE
Sbjct: 1   MEIAGEDGSKLVLREPVTIFGRGSGFASKDRTISRRHILIEAKTSDNTNGAPMEPKVSFE 60

Query: 61  VIGRNPIWVRSGKNGEIRTFRRSEKGEIAPGESFCVGGQEPTWFKLNKIAEIEEGKRIVP 120
           VIGRNPIWVRS KNGEIRTFRRSEKG++APGESFCVGGQEP WF+LNKI E EEGK +  
Sbjct: 61  VIGRNPIWVRSSKNGEIRTFRRSEKGKMAPGESFCVGGQEPIWFELNKITEFEEGKLV-- 120

Query: 121 KTEDQFAERLTRSSDSEDIDVSGIDPVKEFGFLVMGHEFECYGNRVIRDAKKWDWFLDEP 180
                     +RSSDS+DIDVSGIDPVKEFGFLVMGHEFECYGNRVIRD KKWDWFLD P
Sbjct: 121 ---------SSRSSDSDDIDVSGIDPVKEFGFLVMGHEFECYGNRVIRDVKKWDWFLDGP 180

Query: 181 RKGS-DDDEDYGRRKRRGVRGKRKKGANSDDEDWTDENEDAVEMIAKAKKVQRPKYSTRS 240
            K S DDDEDY R+K+RGVRGKRKKGANSDDEDWTDENEDAV+MI K +K QRPKYSTRS
Sbjct: 181 SKDSHDDDEDYERKKKRGVRGKRKKGANSDDEDWTDENEDAVQMITKVQKSQRPKYSTRS 240

Query: 241 KDHNKTNKDQNKAANSNKDYD---EEDEDDETLGGFIVDDEVDNEEQMDEDEEEEFSDDD 300
           KD NK + DQ KAA SNKDYD   EEDEDDETLGGFIVDDEVDN EQMDEDEEEEF DD+
Sbjct: 241 KDRNKISNDQRKAAKSNKDYDGEVEEDEDDETLGGFIVDDEVDNVEQMDEDEEEEFLDDE 292

BLAST of MS016161 vs. ExPASy TrEMBL
Match: A0A1S3BHB4 (nucleolin 1 OS=Cucumis melo OX=3656 GN=LOC103490042 PE=4 SV=1)

HSP 1 Score: 461.8 bits (1187), Expect = 2.1e-126
Identity = 243/303 (80.20%), Postives = 264/303 (87.13%), Query Frame = 0

Query: 1   MEIAGEDGSKLVVGEPVTIFGRGSGFASKDRTVSRRHLLIEAKTSENCDGAPVEPWISFE 60
           MEIAGEDGSKLV+ EPVTIFGRGSGFASKDRT+SRRH+LIEAKTS+N +GAP+EP +SFE
Sbjct: 1   MEIAGEDGSKLVLREPVTIFGRGSGFASKDRTISRRHILIEAKTSDNTNGAPMEPKVSFE 60

Query: 61  VIGRNPIWVRSGKNGEIRTFRRSEKGEIAPGESFCVGGQEPTWFKLNKIAEIEEGKRIVP 120
           VIGRNPIWVRS KNGEIRTFRRSEKG++APGESFCVGGQEP WF+LNKI E EEGK +  
Sbjct: 61  VIGRNPIWVRSSKNGEIRTFRRSEKGKMAPGESFCVGGQEPIWFELNKITEFEEGKLV-- 120

Query: 121 KTEDQFAERLTRSSDSEDIDVSGIDPVKEFGFLVMGHEFECYGNRVIRDAKKWDWFLDEP 180
                     +RSSDS+DIDVSGIDPVKEFGFLVMGHEFECYGNRVIRD KKWDWFLD P
Sbjct: 121 ---------SSRSSDSDDIDVSGIDPVKEFGFLVMGHEFECYGNRVIRDVKKWDWFLDGP 180

Query: 181 RKGS-DDDEDYGRRKRRGVRGKRKKGANSDDEDWTDENEDAVEMIAKAKKVQRPKYSTRS 240
            K S DDDEDY R+K+RGVRGKRKKGANSDDEDWTDENEDAV+MI K +K QRPKYSTRS
Sbjct: 181 SKDSHDDDEDYERKKKRGVRGKRKKGANSDDEDWTDENEDAVQMITKVQKSQRPKYSTRS 240

Query: 241 KDHNKTNKDQNKAANSNKDYD---EEDEDDETLGGFIVDDEVDNEEQMDEDEEEEFSDDD 300
           KD NK + DQ KAA SNKDYD   EEDEDDETLGGFIVDDEVDN EQMDEDEEEEF DD+
Sbjct: 241 KDRNKISNDQRKAAKSNKDYDGEVEEDEDDETLGGFIVDDEVDNVEQMDEDEEEEFLDDE 292

BLAST of MS016161 vs. ExPASy TrEMBL
Match: A0A5A7T1L8 (Nucleolin 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold510G00450 PE=4 SV=1)

HSP 1 Score: 461.5 bits (1186), Expect = 2.8e-126
Identity = 242/303 (79.87%), Postives = 264/303 (87.13%), Query Frame = 0

Query: 1   MEIAGEDGSKLVVGEPVTIFGRGSGFASKDRTVSRRHLLIEAKTSENCDGAPVEPWISFE 60
           MEIAGEDGSKL++ EPVTIFGRGSGFASKDRT+SRRH+LIEAKTS+N +GAP+EP +SFE
Sbjct: 1   MEIAGEDGSKLILREPVTIFGRGSGFASKDRTISRRHILIEAKTSDNTNGAPMEPKVSFE 60

Query: 61  VIGRNPIWVRSGKNGEIRTFRRSEKGEIAPGESFCVGGQEPTWFKLNKIAEIEEGKRIVP 120
           VIGRNPIWVRS KNGEIRTFRRSEKG++APGESFCVGGQEP WF+LNKI E EEGK +  
Sbjct: 61  VIGRNPIWVRSSKNGEIRTFRRSEKGKMAPGESFCVGGQEPIWFELNKITEFEEGKLV-- 120

Query: 121 KTEDQFAERLTRSSDSEDIDVSGIDPVKEFGFLVMGHEFECYGNRVIRDAKKWDWFLDEP 180
                     +RSSDS+DIDVSGIDPVKEFGFLVMGHEFECYGNRVIRD KKWDWFLD P
Sbjct: 121 ---------SSRSSDSDDIDVSGIDPVKEFGFLVMGHEFECYGNRVIRDVKKWDWFLDGP 180

Query: 181 RKGS-DDDEDYGRRKRRGVRGKRKKGANSDDEDWTDENEDAVEMIAKAKKVQRPKYSTRS 240
            K S DDDEDY R+K+RGVRGKRKKGANSDDEDWTDENEDAV+MI K +K QRPKYSTRS
Sbjct: 181 SKDSHDDDEDYERKKKRGVRGKRKKGANSDDEDWTDENEDAVQMITKVQKSQRPKYSTRS 240

Query: 241 KDHNKTNKDQNKAANSNKDYD---EEDEDDETLGGFIVDDEVDNEEQMDEDEEEEFSDDD 300
           KD NK + DQ KAA SNKDYD   EEDEDDETLGGFIVDDEVDN EQMDEDEEEEF DD+
Sbjct: 241 KDRNKISNDQRKAAKSNKDYDGEVEEDEDDETLGGFIVDDEVDNVEQMDEDEEEEFLDDE 292

BLAST of MS016161 vs. ExPASy TrEMBL
Match: A0A6J1J012 (protein BFR2 OS=Cucurbita maxima OX=3661 GN=LOC111479979 PE=4 SV=1)

HSP 1 Score: 458.4 bits (1178), Expect = 2.3e-125
Identity = 240/300 (80.00%), Postives = 262/300 (87.33%), Query Frame = 0

Query: 1   MEIAGEDGSKLVVGEPVTIFGRGSGFASKDRTVSRRHLLIEAKTSENCDGAPVEPWISFE 60
           MEIAGEDGSKLV+ EPVTIFGRGSGFA KDRTVSR H+LI+AKTSEN +G P+EP +SFE
Sbjct: 1   MEIAGEDGSKLVLREPVTIFGRGSGFACKDRTVSRCHILIKAKTSENSNGVPMEPRVSFE 60

Query: 61  VIGRNPIWVRSGKNGEIRTFRRSEKGEIAPGESFCVGGQEPTWFKLNKIAEIEEGKRIVP 120
           VIG+NPIWVRSGKNGEIRTFR SEKGE+ PGESFC+GGQEP WF+LNKIAE EEG++I  
Sbjct: 61  VIGKNPIWVRSGKNGEIRTFRSSEKGEMVPGESFCMGGQEPMWFQLNKIAEFEEGEKI-- 120

Query: 121 KTEDQFAERLTRSSDSEDIDVSGIDPVKEFGFLVMGHEFECYGNRVIRDAKKWDWFLDEP 180
                     +RSSDS+DIDVSGIDPVKEFGFL  GHEFECYGNRVIRDAKKWDWFLDE 
Sbjct: 121 ---------CSRSSDSDDIDVSGIDPVKEFGFLAKGHEFECYGNRVIRDAKKWDWFLDER 180

Query: 181 RKGSDDDEDYGRRKRRGVRGKRKKGANSDDEDWTDENEDAVEMIAKAKKVQRPKYSTRSK 240
           RK SDD+EDYGR+++  VRGKRKKGAN+DDEDWTDENEDAVEMI K KK QRPKYSTRSK
Sbjct: 181 RKDSDDEEDYGRKQKSRVRGKRKKGANNDDEDWTDENEDAVEMITKTKKFQRPKYSTRSK 240

Query: 241 DHNKTNKDQNKAANSNKDYDEEDEDDETLGGFIVDDEVDNEEQMDEDE-EEEFSDDDDED 300
           D NKT+KDQ KAA SNKDY EEDEDDETLGGFIVDDEVDNEE MDEDE EEEFSDD ++D
Sbjct: 241 DRNKTHKDQKKAAKSNKDYVEEDEDDETLGGFIVDDEVDNEELMDEDEDEEEFSDDKEDD 289

BLAST of MS016161 vs. TAIR 10
Match: AT3G13780.1 (SMAD/FHA domain-containing protein )

HSP 1 Score: 214.5 bits (545), Expect = 1.1e-55
Identity = 135/313 (43.13%), Postives = 199/313 (63.58%), Query Frame = 0

Query: 1   MEIAGEDGSKLVVGEPV-TIFGRGSGFASKDRTVSRRHLLIEAKTSENCDGAPVEPWISF 60
           MEI GEDG+KL +   V  +FGRG+GF ++D TVSRRH+ +  K +    G      +SF
Sbjct: 14  MEIEGEDGTKLFLKTGVKDVFGRGAGFNTEDLTVSRRHVSLVFKPA--AAGTEPSDMVSF 73

Query: 61  EVIGRNPIWVRSGKNG-EIRTFRRSEKGEIAPGESFCVGGQEPTWFKLNKIAEIEEGKRI 120
           EV+GRNP+W+R+ + G +I+T R+ E GEIA G+ FCV G  P WF L +  E+ E    
Sbjct: 74  EVLGRNPVWIRAVEKGKKIQTLRKPETGEIATGDQFCVSGNHPIWFTLKRRDEVME---- 133

Query: 121 VPKTEDQFAERLTRSSDSEDIDVSGIDPVKEFGFLVMGHEFECYGNRVIRDAKKWDWFLD 180
                        R+ D  +I+++ IDPVKEFGFLV+G EF+ Y    +RD K+W+WFL+
Sbjct: 134 ------------ERALDHGEIELN-IDPVKEFGFLVIGKEFDQYPKSRVRDIKQWEWFLE 193

Query: 181 EPRKG-SDDDEDYGRRKRRGVRGKRKKGANSDDEDWTDENEDAVEMIAKAKKVQRPKYST 240
           +   G SD DED  ++ R+G+  KR++   ++D+DW+ E+++  E++ K+K+V  P YST
Sbjct: 194 DSTNGNSDGDEDGDKKGRKGLGKKRRRKKGNEDDDWSVESDEDKELMVKSKRVVTPTYST 253

Query: 241 RSKDHNKTNKDQNKAANSNK----------DYDEEDEDDETLGGFIV-DDEVDNEEQMDE 300
           RSK   KT KD N +++S+           D +EED+DDETLGGFIV D+E   EE+ +E
Sbjct: 254 RSK---KTKKDSNASSSSSNGAQTKQRGRADVEEEDDDDETLGGFIVSDEEAKLEEEEEE 304

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022152655.19.0e-164100.00protein bfr2 [Momordica charantia][more]
XP_038898830.16.7e-12780.78protein PIF [Benincasa hispida][more]
XP_008447630.14.4e-12680.20PREDICTED: nucleolin 1 [Cucumis melo] >TYK03672.1 nucleolin 1 [Cucumis melo var.... [more]
KAA0036698.15.7e-12679.87nucleolin 1 [Cucumis melo var. makuwa][more]
XP_022980683.14.8e-12580.00protein BFR2 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1DEJ64.4e-164100.00protein bfr2 OS=Momordica charantia OX=3673 GN=LOC111020320 PE=4 SV=1[more]
A0A5D3BXV52.1e-12680.20Nucleolin 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold863G00290 PE... [more]
A0A1S3BHB42.1e-12680.20nucleolin 1 OS=Cucumis melo OX=3656 GN=LOC103490042 PE=4 SV=1[more]
A0A5A7T1L82.8e-12679.87Nucleolin 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold510G00450 PE... [more]
A0A6J1J0122.3e-12580.00protein BFR2 OS=Cucurbita maxima OX=3661 GN=LOC111479979 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT3G13780.11.1e-5543.13SMAD/FHA domain-containing protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D2.60.200.20coord: 3..110
e-value: 1.7E-11
score: 46.0
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 259..299
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 219..258
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 182..299
NoneNo IPR availablePANTHERPTHR37733SMAD/FHA DOMAIN-CONTAINING PROTEINcoord: 1..299
IPR008984SMAD/FHA domain superfamilySUPERFAMILY49879SMAD/FHA domaincoord: 15..97

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS016161.1MS016161.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005515 protein binding