MS016140 (gene) Bitter gourd (TR) v1

BLAST of MS016140 vs. NCBI nr
Match: XP_022152666.1 (protein BIG GRAIN 1-like B [Momordica charantia])

HSP 1 Score: 644.8 bits (1662), Expect = 4.1e-181
Identity = 338/339 (99.71%), Postives = 338/339 (99.71%), Query Frame = 0

Query: 1   FNLWDKPQPLQREDRDHRRRLRRENPSFSSSLLDAIYRSIDESNGQGEEPQLILYRETTM 60
           FNLWDKPQPLQRE RDHRRRLRRENPSFSSSLLDAIYRSIDESNGQGEEPQLILYRETTM
Sbjct: 3   FNLWDKPQPLQREARDHRRRLRRENPSFSSSLLDAIYRSIDESNGQGEEPQLILYRETTM 62

Query: 61  TKQSIAAARTEDADEALKFRRVEKWMEMKKKKVGSNVRDFTNSSSSSSESSCTAGRFSSS 120
           TKQSIAAARTEDADEALKFRRVEKWMEMKKKKVGSNVRDFTNSSSSSSESSCTAGRFSSS
Sbjct: 63  TKQSIAAARTEDADEALKFRRVEKWMEMKKKKVGSNVRDFTNSSSSSSESSCTAGRFSSS 122

Query: 121 ESEFLSRPKPIKTTTEIPKPKPKHENGFLKTKSKASKMYHDLKKVKQPISPGARLANFLN 180
           ESEFLSRPKPIKTTTEIPKPKPKHENGFLKTKSKASKMYHDLKKVKQPISPGARLANFLN
Sbjct: 123 ESEFLSRPKPIKTTTEIPKPKPKHENGFLKTKSKASKMYHDLKKVKQPISPGARLANFLN 182

Query: 181 SLFNGGTPKTKQKLSNSSVQSSKSLQGSTCSSTSSFSRSCLSKTPSSRGNAAKRSVRFCP 240
           SLFNGGTPKTKQKLSNSSVQSSKSLQGSTCSSTSSFSRSCLSKTPSSRGNAAKRSVRFCP
Sbjct: 183 SLFNGGTPKTKQKLSNSSVQSSKSLQGSTCSSTSSFSRSCLSKTPSSRGNAAKRSVRFCP 242

Query: 241 VSVIVDEDCRPCGHKILHKIEPILKKPLKTEQVKTEHDFEDDDEDYDDEDDDGLSCSSSD 300
           VSVIVDEDCRPCGHKILHKIEPILKKPLKTEQVKTEHDFEDDDEDYDDEDDDGLSCSSSD
Sbjct: 243 VSVIVDEDCRPCGHKILHKIEPILKKPLKTEQVKTEHDFEDDDEDYDDEDDDGLSCSSSD 302

Query: 301 LFELDNLSVIGIERYREELPVYETTNFKTNCAIANGLVL 340
           LFELDNLSVIGIERYREELPVYETTNFKTNCAIANGLVL
Sbjct: 303 LFELDNLSVIGIERYREELPVYETTNFKTNCAIANGLVL 341

BLAST of MS016140 vs. NCBI nr
Match: XP_038903204.1 (protein BIG GRAIN 1-like B [Benincasa hispida])

HSP 1 Score: 407.5 bits (1046), Expect = 1.1e-109
Identity = 254/366 (69.40%), Postives = 285/366 (77.87%), Query Frame = 0

Query: 14  DRDHRRRLRRENPSFSSSLLDAIYRSIDESNGQGEEPQLILYRETTM-TKQSI-AAARTE 73
           D   RRR RRENPSFSSSLLDAIYRSIDESN Q E+  ++  ++TT+ TKQSI +AARTE
Sbjct: 13  DNPDRRRSRRENPSFSSSLLDAIYRSIDESNAQPEDHLILYTQKTTLRTKQSISSAARTE 72

Query: 74  DADEALKFRR---VEKWMEMKKKKVGSNVRDFTNSSSSSSESSCTAG-RFSSSESEFLSR 133
           D DE LKF R   ++KWM+ KK +   N+RDF+ SSSSSSESS TAG RFSSSESEFLSR
Sbjct: 73  D-DETLKFHRACIIDKWMDKKKLR---NIRDFSLSSSSSSESSSTAGRRFSSSESEFLSR 132

Query: 134 --------PKPIKTT----TEIPKPKPKHENGFLKTKSKASKMYHDLKKVKQPISPGARL 193
                   PKPIKTT    TEI  PKPKHENGF+K+KSKASK+YHDLKKVKQPISPG RL
Sbjct: 133 ALHRPKPKPKPIKTTTWAQTEIISPKPKHENGFVKSKSKASKIYHDLKKVKQPISPGGRL 192

Query: 194 ANFLNSLFNGGTPKTKQKLSNSS-------------VQSSKSLQGSTCSSTSSFSRSCLS 253
           A+FLNSLFNGG+PKTKQK+SNSS                S+ +  STCSS SSFSRSCLS
Sbjct: 193 ASFLNSLFNGGSPKTKQKISNSSSINSTNFDYDMSRKSKSQQVSTSTCSSASSFSRSCLS 252

Query: 254 KTPSSRGNAAKRSVRFCPVSVIVDEDCRPCGHKILHKI-EPILKK-------PLKTEQVK 313
           KTPSSRGN  KRSVRFCPVSVIVDEDC+PCG KILHK+ EPI+KK         +TE+VK
Sbjct: 253 KTPSSRGN-IKRSVRFCPVSVIVDEDCQPCGRKILHKLEEPIMKKGNLKSYRNFETEKVK 312

Query: 314 TEHDFEDDDE-DYDDEDDDGLSCSSSDLFELDNLSVIGIERYREELPVYETTNFKTNCAI 340
           TEH+ + + E D DD+DDD LSCSSSDLFELDNLSVIGIERYREELPVYETT+F TNCAI
Sbjct: 313 TEHEMKINYELDEDDDDDDALSCSSSDLFELDNLSVIGIERYREELPVYETTHFNTNCAI 372

BLAST of MS016140 vs. NCBI nr
Match: XP_022948938.1 (protein BIG GRAIN 1-like B [Cucurbita moschata])

HSP 1 Score: 405.6 bits (1041), Expect = 4.2e-109
Identity = 251/373 (67.29%), Postives = 281/373 (75.34%), Query Frame = 0

Query: 2   NLWDKPQPLQREDRDHRRRLRRENPSFSSSLLDAIYRSIDESNGQGEEPQLILYRETTM- 61
           +LWDK      +D   RRR RR+NPSFSSSLLDAIYRSIDESN Q E+  L  YRETTM 
Sbjct: 2   DLWDKSFQRHDQDLPQRRRSRRDNPSFSSSLLDAIYRSIDESNDQDEDGHLFFYRETTMR 61

Query: 62  TKQSIAAARTEDADEALKFRR---VEKWMEMKKKKVGSNVRDFTNSSSSSSESSCTAG-R 121
           TKQS+  ARTED DEA+KFRR   +EKWM+ KK +   N+RDFT SSSSSSES+ TAG R
Sbjct: 62  TKQSVVTARTED-DEAVKFRRACMIEKWMDRKKVR---NIRDFTVSSSSSSESTSTAGRR 121

Query: 122 FSSSESEFLS----RPKPIKTT--TEIPKPKPKHENGFLKTKSKASKMYHDLKKVKQPIS 181
           +S+SESE LS    R KPIKTT  T+    K KHENG +KTKSKASK+YHDLKKVKQPIS
Sbjct: 122 YSASESELLSRALHRQKPIKTTPWTQTETQKAKHENGLVKTKSKASKIYHDLKKVKQPIS 181

Query: 182 PGARLANFLNSLFNGGTPKTKQKLSNSS---------VQSSKSLQGSTCSSTSSFSRSCL 241
           PG RLANFLNSLFNGG+PKTKQK+ N S         + +    QGSTCSS SSFSRSCL
Sbjct: 182 PGGRLANFLNSLFNGGSPKTKQKMPNPSTNPRSMDYDMPTKAKSQGSTCSSASSFSRSCL 241

Query: 242 SKTPSSRGNAAKRSVRFCPVSVIVDEDCRPCGHKILHKI-EPILKKPLK------TEQVK 301
           SKTPSSRGN  KRSVRFCPVS IVDEDC   GHK L K+ EPI+KK LK      TE+VK
Sbjct: 242 SKTPSSRGN-VKRSVRFCPVSEIVDEDCEAYGHKSLQKLEEPIMKKGLKSYGKFETEEVK 301

Query: 302 TEH--------DFEDDDEDYDDEDDDGLSCSSSDLFELDNLSVIGIERYREELPVYETTN 340
           TEH         FE +DED ++E+DD +SCSSSDLFELDNLSVIG+ERYREELPVYETTN
Sbjct: 302 TEHPRKSKGNEGFEYEDEDEEEEEDDAVSCSSSDLFELDNLSVIGMERYREELPVYETTN 361

BLAST of MS016140 vs. NCBI nr
Match: XP_023525389.1 (protein BIG GRAIN 1-like B [Cucurbita pepo subsp. pepo])

HSP 1 Score: 404.4 bits (1038), Expect = 9.4e-109
Identity = 250/373 (67.02%), Postives = 280/373 (75.07%), Query Frame = 0

Query: 2   NLWDKPQPLQREDRDHRRRLRRENPSFSSSLLDAIYRSIDESNGQGEEPQLILYRETTM- 61
           +LWDK      +D   RRR RR+NPSFSSSLLDAIYRSIDESN Q E+  L  YRETTM 
Sbjct: 2   DLWDKSFQRHDQDLPQRRRSRRDNPSFSSSLLDAIYRSIDESNDQDEDGHLFFYRETTMR 61

Query: 62  TKQSIAAARTEDADEALKFRR---VEKWMEMKKKKVGSNVRDFTNSSSSSSESSCTAG-R 121
           TKQS+  ARTED DEA+KFRR   +EKWM+ KK +   N+RDFT SSSSSSES+ TAG R
Sbjct: 62  TKQSVVTARTED-DEAVKFRRACMIEKWMDRKKVR---NIRDFTVSSSSSSESTSTAGRR 121

Query: 122 FSSSESEFLS----RPKPIKTT--TEIPKPKPKHENGFLKTKSKASKMYHDLKKVKQPIS 181
           +S+SESE LS    RPKPIKTT  T+    K KHENG +KTKSKASKMYHDLKKVKQPIS
Sbjct: 122 YSASESELLSRALHRPKPIKTTPWTQTETQKAKHENGLVKTKSKASKMYHDLKKVKQPIS 181

Query: 182 PGARLANFLNSLFNGGTPKTKQKLSNSS---------VQSSKSLQGSTCSSTSSFSRSCL 241
           PG RLANFLNSLFNGG+PKTKQK  N S         + +    QGSTCSS SSFSRSCL
Sbjct: 182 PGGRLANFLNSLFNGGSPKTKQKTPNRSTNPRSMDYDMPTKAKSQGSTCSSASSFSRSCL 241

Query: 242 SKTPSSRGNAAKRSVRFCPVSVIVDEDCRPCGHKILHKI-EPILKKPLK------TEQVK 301
           SKTP SRGN  KRSVRFCPVS IVDEDC   GHK L K+ EPI+KK LK      TE+VK
Sbjct: 242 SKTPCSRGN-VKRSVRFCPVSEIVDEDCEADGHKSLQKLEEPIMKKGLKSYGKFETEEVK 301

Query: 302 TEH--------DFEDDDEDYDDEDDDGLSCSSSDLFELDNLSVIGIERYREELPVYETTN 340
           TEH         F+ +DED ++E+DD +SCSSSDLFELDNLSVIG+ERYREELPVYETTN
Sbjct: 302 TEHPRKSKGNEGFDYEDEDEEEEEDDAVSCSSSDLFELDNLSVIGMERYREELPVYETTN 361

BLAST of MS016140 vs. NCBI nr
Match: KAG6606698.1 (Protein BIG GRAIN 1-like A, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 399.1 bits (1024), Expect = 3.9e-107
Identity = 249/373 (66.76%), Postives = 279/373 (74.80%), Query Frame = 0

Query: 2   NLWDKPQPLQREDRDHRRRLRRENPSFSSSLLDAIYRSIDESNGQGEEPQLILYRETTM- 61
           +LWDK      +D   RRR RR+NPSFSSSLLDAIYRSIDESN Q E+  L  YRETTM 
Sbjct: 2   DLWDKSFQRHDQDLPQRRRSRRDNPSFSSSLLDAIYRSIDESNDQDEDGHLFFYRETTMR 61

Query: 62  TKQSIAAARTEDADEALKFRR---VEKWMEMKKKKVGSNVRDFTNSSSSSSESSCTAG-R 121
           TKQS+  ARTED DEA+KFRR   +EKWM+ KK +   N+RDFT SSSSSSES+ TAG R
Sbjct: 62  TKQSVVTARTED-DEAVKFRRACMIEKWMDRKKVR---NIRDFTVSSSSSSESTSTAGRR 121

Query: 122 FSSSESEFLS----RPKPIKTT--TEIPKPKPKHENGFLKTKSKASKMYHDLKKVKQPIS 181
           +S+SESE LS    R KPIKTT  T+    K KHENG +KTKSKASK+YHDLKKVKQPIS
Sbjct: 122 YSASESELLSRALHRQKPIKTTPWTQTETQKAKHENGLVKTKSKASKVYHDLKKVKQPIS 181

Query: 182 PGARLANFLNSLFNGGTPKTKQKLSNSS---------VQSSKSLQGSTCSSTSSFSRSCL 241
           PG RLANFLNSLFNGG+PKTKQK+ N S         + +    QGSTCSS SSFSRSCL
Sbjct: 182 PGGRLANFLNSLFNGGSPKTKQKIPNPSTNPRSMDYDMATKAKSQGSTCSSASSFSRSCL 241

Query: 242 SKTPSSRGNAAKRSVRFCPVSVIVDEDCRPCGHKILHKI-EPILKKPLK------TEQVK 301
           SKTPSSRGN  KRSVRFCPVS IV+EDC   G K L K+ EPI+KK LK      TE+VK
Sbjct: 242 SKTPSSRGN-VKRSVRFCPVSEIVEEDCEAYGQKSLQKLEEPIMKKGLKSYGKFETEEVK 301

Query: 302 TEH--------DFEDDDEDYDDEDDDGLSCSSSDLFELDNLSVIGIERYREELPVYETTN 340
           TEH         FE +DED ++E+DD  SCSSSDLFELDNLSVIG+ERYREELPVYETTN
Sbjct: 302 TEHPRKSKGNEGFEYEDEDEEEEEDDAGSCSSSDLFELDNLSVIGMERYREELPVYETTN 361

BLAST of MS016140 vs. ExPASy Swiss-Prot
Match: Q9SLL2 (Protein BIG GRAIN 1-like B OS=Arabidopsis thaliana OX=3702 GN=At1g54200 PE=1 SV=1)

HSP 1 Score: 211.1 bits (536), Expect = 2.0e-53
Identity = 166/374 (44.39%), Postives = 211/374 (56.42%), Query Frame = 0

Query: 14  DRDHRRRLRRE--NPSFSSSLLDAIYRSIDESNGQGEEPQLILYRETTMTKQSIAAARTE 73
           D  HR R RR+  +PSFSS+LLD IYRSID+S+      +   ++    T+  ++A R +
Sbjct: 12  DHQHRLRNRRDHRHPSFSSTLLDQIYRSIDDSSTNSSSMRKTKHQNREDTR--VSANRRD 71

Query: 74  DADEALKFRRVEKWMEMKKKKVGSNVRDFTNSSSSSSESSCTAGRFSSSESEF------- 133
           D + +   + +E                F +SSSSSS+SS     FSSSES++       
Sbjct: 72  DFNRSKNLKTIEPVF-------------FKHSSSSSSDSS----GFSSSESDYFYRRSKS 131

Query: 134 ---LSRPKPIKTTTE---------IPKPKPKHENG-FLKTKSKASKMYHDLKKVKQPISP 193
              +S PKPI+TT E          P    K E+G FLKTKSKA K+Y DLKKVKQPISP
Sbjct: 132 SPAISHPKPIRTTVERFERSPQNHRPNSSNKQEHGSFLKTKSKALKIYSDLKKVKQPISP 191

Query: 194 GARLANFLNSLFNGGTPKTKQKLSNSSVQSSKSLQG-----STCSSTSSFSRSCLSKTPS 253
           G RLA FLNS+F G     K    N++V S+ +        +TCSS SSFSRSCLSKTPS
Sbjct: 192 GGRLATFLNSIFTGAGNTKKLNKINTTVTSTTAAAAAASSTTTCSSASSFSRSCLSKTPS 251

Query: 254 SRGNAAKRSVRFCPVSVIVDEDCRPCGHK--------------ILHKIE----------- 313
           S    +KRSVRFCPV+VI DED     +K              I H +E           
Sbjct: 252 S-SEKSKRSVRFCPVNVIFDEDSSKYNNKNNKVYGNNEREYESIRHTLENRVMEENRRVI 311

Query: 314 PILKKPLKTEQVKTEHDFE-DDDEDYDDEDDDGLSCSSSDLFELDNLSVIGIERYREELP 335
              K+ L++ Q K +   E   ++D +D+DDD LSC+SSDLFELDNLS IGI+RYREELP
Sbjct: 312 EAAKELLRSYQKKNKEVIEVSVEDDEEDDDDDALSCTSSDLFELDNLSAIGIDRYREELP 365

BLAST of MS016140 vs. ExPASy Swiss-Prot
Match: Q9LVK2 (Protein BIG GRAIN 1-like A OS=Arabidopsis thaliana OX=3702 GN=At3g13980 PE=3 SV=1)

HSP 1 Score: 196.8 bits (499), Expect = 3.9e-49
Identity = 162/367 (44.14%), Postives = 206/367 (56.13%), Query Frame = 0

Query: 4   WDKPQPLQREDRDHRRRLRRENPSFSSSLLDAIYRSIDESNGQGEEPQLILYRETTMTKQ 63
           W+KP     +   H R     NPSFSS+LLD IYRSID+S+     P L   ++     Q
Sbjct: 5   WEKP-----KSSSHHR-----NPSFSSTLLDQIYRSIDDSS---PPPPLESIKKKKHHHQ 64

Query: 64  SIAAARTEDADEALKFRRVEKWMEMKKKKVGSNVRDFTNSSS------SSSESSCTAGRF 123
              A+  ED + +  + R     + ++ +  ++    +NSSS      SSSES    GR 
Sbjct: 65  QRNASLHEDREISPIYHRRSIAADFERSRRKTDFLRHSNSSSSDSSGFSSSESDSFHGRS 124

Query: 124 SSSESEFLS---RPKPIKTTT---EIPKPKPKHENGFLKTKSKASKMYHDLKKVKQPISP 183
            SS S   S   +PKPI+T++        KPK   GFL+TKSKA K+Y DLKKVKQPISP
Sbjct: 125 KSSASPPSSSRQQPKPIRTSSVDHSSAVQKPKELGGFLRTKSKALKIYSDLKKVKQPISP 184

Query: 184 GARLANFLNSLF-NGGTPKTKQKLSNSSVQSSKSLQGSTCSSTSSFSRSCLSKTPSSRGN 243
           G RLA FLNSLF N  T   K K + +     +    STCSS SSFSRSCLSKTPSS G 
Sbjct: 185 GGRLATFLNSLFTNAATNPKKHKKTTTVAVVEEPHSSSTCSSASSFSRSCLSKTPSSSGK 244

Query: 244 AAKRSVRFCPVSVIVDEDC-----------RPCGHKILHKIE------PILKKPLKTEQV 303
            +KRSVRFCPV+VI+DED            R   +    ++E         K  L+T   
Sbjct: 245 -SKRSVRFCPVNVILDEDSSFTMPYAYNNERLYDNNEAKRVEEHRRVIQAAKDLLRTYHN 304

Query: 304 KTEHDFEDDDEDYDDEDDDGLSCSSSDLFELDNLSVIGIERYREELPVYETTNF-KTNCA 340
           K +    + +   +D++DD  SC+SSDLFEL+NLS IGIERYREELPVYETT     N  
Sbjct: 305 KNKVTTTNINNVEEDDEDDAASCASSDLFELENLSAIGIERYREELPVYETTRLDNMNRV 357

BLAST of MS016140 vs. ExPASy Swiss-Prot
Match: Q9M2B3 (Protein BIG GRAIN 1-like C OS=Arabidopsis thaliana OX=3702 GN=At3g42800 PE=2 SV=1)

HSP 1 Score: 126.7 bits (317), Expect = 4.9e-28
Identity = 121/344 (35.17%), Postives = 178/344 (51.74%), Query Frame = 0

Query: 22  RRENPSFSSSLLDAIYRSIDESNGQGEEPQLILYRETTMTKQSIAAARTEDADEALK--F 81
           R+  PSFSSS+LD++YRSIDES+G   + +  +    + +  S +  + +D    L+   
Sbjct: 6   RKRTPSFSSSVLDSVYRSIDESDGLQSDLKGSINENVSSSSSSPSPNKKDDKLTTLRRAI 65

Query: 82  RRVEKWMEMKKKKVGSNVRDFTNSSSSSSESSCTAGRFS--SSESEFLSRPKPIKTTTEI 141
              E W+  +     +N  D ++ SSS +ES  T  R    + + +     +     T +
Sbjct: 66  MDEEHWLYARSSTTTTNSSDSSSFSSSEAESYRTKRRLRKLAEQGKRSGDERQRTKRTVM 125

Query: 142 PKPKPKHENGFLKTKSKASKMYHDLKKVKQPISPGARLANFLNSLFNGGTPKTKQKLSNS 201
                         K KA K+  +LK+ KQP+SPGARL +FLNS+F     K K      
Sbjct: 126 DNDSRLFSKSDDDKKPKAVKIIEELKRSKQPVSPGARLTSFLNSIFQSNAKKVKL----C 185

Query: 202 SVQSSKSLQGSTCSSTSSFSRSCLSKTPSSRGNAAK--RSVRFCPVSVIVDEDCRPCGHK 261
           SV  +  ++ S  SS S FSR+  +KT ++  N  K  RS+RF PV V +D DCR    K
Sbjct: 186 SVGKTTDVKSS--SSKSCFSRT-RNKTDNNNNNCKKLERSIRFYPVRVTIDGDCRDYAQK 245

Query: 262 ILHKI-EPI--------LKKPLKTEQVKTE----------HDF-EDDDEDYDDEDDDGLS 321
            + ++ +PI        +K+ +KT    TE           DF   +  +  +E++D  S
Sbjct: 246 HITRVRKPIPEFTAKKSVKEEIKTNDHHTEFTCITRNIGLKDFVRSNKYEGKEEEEDAWS 305

Query: 322 CSSSDLFELDNLSVIGIERYREELPVYETTNFKTNCAIANGLVL 340
            SSSDLFELD+   IG+ RY +ELPVYETT+FKTN AIA  L+L
Sbjct: 306 HSSSDLFELDSYR-IGMGRYLKELPVYETTDFKTNQAIARSLLL 341

BLAST of MS016140 vs. ExPASy Swiss-Prot
Match: Q10R09 (Protein BIG GRAIN 1 OS=Oryza sativa subsp. japonica OX=39947 GN=BG1 PE=1 SV=1)

HSP 1 Score: 124.4 bits (311), Expect = 2.5e-27
Identity = 123/331 (37.16%), Postives = 171/331 (51.66%), Query Frame = 0

Query: 19  RRLRRENPSFSSSLLDAIYRSIDESNGQGEEPQLILYRETTMTKQSIAAARTEDADEALK 78
           RR   + PSFSS+LLDAIY+S+DE  G G     +         +++AAA  +  + AL 
Sbjct: 15  RRYVADQPSFSSTLLDAIYKSMDEQPGHGGGATGV---------EAVAAAAKKQHEAALH 74

Query: 79  FRRVEKWMEMKKKKVGS-NVRDFTNSSSSSSESSCTA-GRFSSSESE--FLSRPKPIKTT 138
           +         K    GS   R     +++SS S C++ G FSSSE+E     R +PI+TT
Sbjct: 75  YGNY-----YKPSLAGSYRARAPGPHATTSSSSECSSYGGFSSSEAESSHHRRLRPIRTT 134

Query: 139 TEIPKPKPKHENGFLKTKSKASKMYHDLKKVKQPISPGARLANFLNSLFNGGTPKTKQKL 198
                P P  E    K K   + +   L+ +++P SPGARLA FLNS+F G       K 
Sbjct: 135 VPGGAPGPAPEK---KAKKPGASIRAKLRDLRKPASPGARLAGFLNSIFAG-------KR 194

Query: 199 SNSSVQSSKSLQGSTCSSTSSFSRSCLSKTPSSRGNAAKRSVRF-------CPVSVIVDE 258
           + ++  S+ +   S CS+ SS+SRSCLSKTPS+RG  AKR+VRF          S +VD 
Sbjct: 195 APATPPSATAGAESACSTASSYSRSCLSKTPSTRGQ-AKRTVRFLDSDTESLASSTVVDR 254

Query: 259 DCRPCGHKILHKIEPILKKPLKTEQVKTEHDFEDDDEDYDDEDDDGLSCSSSDLFELDNL 318
              P     +  ++ +L + ++ E                DEDDD  S +SSDLFEL+N 
Sbjct: 255 RRVP-----VEAVQQMLLQRMEME---------------SDEDDDESSDASSDLFELENF 300

Query: 319 SVIGI--ERYREELPVYETTNFKTNCAIANG 337
           + I      YR+ELPVYETT    N AI +G
Sbjct: 315 AAIAPAGAAYRDELPVYETTRVALNRAIGHG 300

BLAST of MS016140 vs. ExPASy Swiss-Prot
Match: B8APC7 (Protein BIG GRAIN 1 OS=Oryza sativa subsp. indica OX=39946 GN=BG1 PE=3 SV=1)

HSP 1 Score: 124.0 bits (310), Expect = 3.2e-27
Identity = 123/331 (37.16%), Postives = 171/331 (51.66%), Query Frame = 0

Query: 19  RRLRRENPSFSSSLLDAIYRSIDESNGQGEEPQLILYRETTMTKQSIAAARTEDADEALK 78
           RR   + PSFSS+LLDAIY+S+DE  G G     +         +++AAA  +  + AL 
Sbjct: 15  RRYVADQPSFSSTLLDAIYKSMDEQPGHGGGATGV---------EAVAAAAKKQHEAALH 74

Query: 79  FRRVEKWMEMKKKKVGS-NVRDFTNSSSSSSESSCTA-GRFSSSESE--FLSRPKPIKTT 138
           +         K    GS   R     +++SS S C++ G FSSSE+E     R +PI+TT
Sbjct: 75  Y-----GYYYKPSLAGSYRARAPGPHATTSSSSECSSYGGFSSSEAESSHHRRLRPIRTT 134

Query: 139 TEIPKPKPKHENGFLKTKSKASKMYHDLKKVKQPISPGARLANFLNSLFNGGTPKTKQKL 198
                P P  E    K K   + +   L+ +++P SPGARLA FLNS+F G       K 
Sbjct: 135 VPGGAPGPAPEK---KAKKPGASIRAKLRDLRKPASPGARLAGFLNSIFAG-------KR 194

Query: 199 SNSSVQSSKSLQGSTCSSTSSFSRSCLSKTPSSRGNAAKRSVRF-------CPVSVIVDE 258
           + ++  S+ +   S CS+ SS+SRSCLSKTPS+RG  AKR+VRF          S +VD 
Sbjct: 195 APATPPSATAGAESACSTASSYSRSCLSKTPSTRGQ-AKRTVRFLDSDTESLASSTVVDR 254

Query: 259 DCRPCGHKILHKIEPILKKPLKTEQVKTEHDFEDDDEDYDDEDDDGLSCSSSDLFELDNL 318
              P     +  ++ +L + ++ E                DEDDD  S +SSDLFEL+N 
Sbjct: 255 RRVP-----VEAVQQMLLQRMEME---------------SDEDDDESSDASSDLFELENF 300

Query: 319 SVIGI--ERYREELPVYETTNFKTNCAIANG 337
           + I      YR+ELPVYETT    N AI +G
Sbjct: 315 AAIAPAGAAYRDELPVYETTRVALNRAIGHG 300

BLAST of MS016140 vs. ExPASy TrEMBL
Match: A0A6J1DIF0 (protein BIG GRAIN 1-like B OS=Momordica charantia OX=3673 GN=LOC111020328 PE=3 SV=1)

HSP 1 Score: 644.8 bits (1662), Expect = 2.0e-181
Identity = 338/339 (99.71%), Postives = 338/339 (99.71%), Query Frame = 0

Query: 1   FNLWDKPQPLQREDRDHRRRLRRENPSFSSSLLDAIYRSIDESNGQGEEPQLILYRETTM 60
           FNLWDKPQPLQRE RDHRRRLRRENPSFSSSLLDAIYRSIDESNGQGEEPQLILYRETTM
Sbjct: 3   FNLWDKPQPLQREARDHRRRLRRENPSFSSSLLDAIYRSIDESNGQGEEPQLILYRETTM 62

Query: 61  TKQSIAAARTEDADEALKFRRVEKWMEMKKKKVGSNVRDFTNSSSSSSESSCTAGRFSSS 120
           TKQSIAAARTEDADEALKFRRVEKWMEMKKKKVGSNVRDFTNSSSSSSESSCTAGRFSSS
Sbjct: 63  TKQSIAAARTEDADEALKFRRVEKWMEMKKKKVGSNVRDFTNSSSSSSESSCTAGRFSSS 122

Query: 121 ESEFLSRPKPIKTTTEIPKPKPKHENGFLKTKSKASKMYHDLKKVKQPISPGARLANFLN 180
           ESEFLSRPKPIKTTTEIPKPKPKHENGFLKTKSKASKMYHDLKKVKQPISPGARLANFLN
Sbjct: 123 ESEFLSRPKPIKTTTEIPKPKPKHENGFLKTKSKASKMYHDLKKVKQPISPGARLANFLN 182

Query: 181 SLFNGGTPKTKQKLSNSSVQSSKSLQGSTCSSTSSFSRSCLSKTPSSRGNAAKRSVRFCP 240
           SLFNGGTPKTKQKLSNSSVQSSKSLQGSTCSSTSSFSRSCLSKTPSSRGNAAKRSVRFCP
Sbjct: 183 SLFNGGTPKTKQKLSNSSVQSSKSLQGSTCSSTSSFSRSCLSKTPSSRGNAAKRSVRFCP 242

Query: 241 VSVIVDEDCRPCGHKILHKIEPILKKPLKTEQVKTEHDFEDDDEDYDDEDDDGLSCSSSD 300
           VSVIVDEDCRPCGHKILHKIEPILKKPLKTEQVKTEHDFEDDDEDYDDEDDDGLSCSSSD
Sbjct: 243 VSVIVDEDCRPCGHKILHKIEPILKKPLKTEQVKTEHDFEDDDEDYDDEDDDGLSCSSSD 302

Query: 301 LFELDNLSVIGIERYREELPVYETTNFKTNCAIANGLVL 340
           LFELDNLSVIGIERYREELPVYETTNFKTNCAIANGLVL
Sbjct: 303 LFELDNLSVIGIERYREELPVYETTNFKTNCAIANGLVL 341

BLAST of MS016140 vs. ExPASy TrEMBL
Match: A0A6J1GAP7 (protein BIG GRAIN 1-like B OS=Cucurbita moschata OX=3662 GN=LOC111452438 PE=3 SV=1)

HSP 1 Score: 405.6 bits (1041), Expect = 2.0e-109
Identity = 251/373 (67.29%), Postives = 281/373 (75.34%), Query Frame = 0

Query: 2   NLWDKPQPLQREDRDHRRRLRRENPSFSSSLLDAIYRSIDESNGQGEEPQLILYRETTM- 61
           +LWDK      +D   RRR RR+NPSFSSSLLDAIYRSIDESN Q E+  L  YRETTM 
Sbjct: 2   DLWDKSFQRHDQDLPQRRRSRRDNPSFSSSLLDAIYRSIDESNDQDEDGHLFFYRETTMR 61

Query: 62  TKQSIAAARTEDADEALKFRR---VEKWMEMKKKKVGSNVRDFTNSSSSSSESSCTAG-R 121
           TKQS+  ARTED DEA+KFRR   +EKWM+ KK +   N+RDFT SSSSSSES+ TAG R
Sbjct: 62  TKQSVVTARTED-DEAVKFRRACMIEKWMDRKKVR---NIRDFTVSSSSSSESTSTAGRR 121

Query: 122 FSSSESEFLS----RPKPIKTT--TEIPKPKPKHENGFLKTKSKASKMYHDLKKVKQPIS 181
           +S+SESE LS    R KPIKTT  T+    K KHENG +KTKSKASK+YHDLKKVKQPIS
Sbjct: 122 YSASESELLSRALHRQKPIKTTPWTQTETQKAKHENGLVKTKSKASKIYHDLKKVKQPIS 181

Query: 182 PGARLANFLNSLFNGGTPKTKQKLSNSS---------VQSSKSLQGSTCSSTSSFSRSCL 241
           PG RLANFLNSLFNGG+PKTKQK+ N S         + +    QGSTCSS SSFSRSCL
Sbjct: 182 PGGRLANFLNSLFNGGSPKTKQKMPNPSTNPRSMDYDMPTKAKSQGSTCSSASSFSRSCL 241

Query: 242 SKTPSSRGNAAKRSVRFCPVSVIVDEDCRPCGHKILHKI-EPILKKPLK------TEQVK 301
           SKTPSSRGN  KRSVRFCPVS IVDEDC   GHK L K+ EPI+KK LK      TE+VK
Sbjct: 242 SKTPSSRGN-VKRSVRFCPVSEIVDEDCEAYGHKSLQKLEEPIMKKGLKSYGKFETEEVK 301

Query: 302 TEH--------DFEDDDEDYDDEDDDGLSCSSSDLFELDNLSVIGIERYREELPVYETTN 340
           TEH         FE +DED ++E+DD +SCSSSDLFELDNLSVIG+ERYREELPVYETTN
Sbjct: 302 TEHPRKSKGNEGFEYEDEDEEEEEDDAVSCSSSDLFELDNLSVIGMERYREELPVYETTN 361

BLAST of MS016140 vs. ExPASy TrEMBL
Match: A0A6J1KB46 (protein BIG GRAIN 1-like B OS=Cucurbita maxima OX=3661 GN=LOC111493335 PE=3 SV=1)

HSP 1 Score: 395.2 bits (1014), Expect = 2.8e-106
Identity = 247/368 (67.12%), Postives = 279/368 (75.82%), Query Frame = 0

Query: 2   NLWDKPQPLQREDRDHRRRLRRENPSFSSSLLDAIYRSIDESNGQGEEPQLILYRETTM- 61
           +LWDK      +D   RRR RR+NPSFSSSLLDAIYRSIDESN Q E+  L  YRETTM 
Sbjct: 85  DLWDKSFQRHDQDLPQRRRSRRDNPSFSSSLLDAIYRSIDESNHQDEDGHLFFYRETTMR 144

Query: 62  TKQSIAAARTEDADEALKFRR---VEKWMEMKKKKVGSNVRDFTNSSSSSSESSCTAG-R 121
           TKQS+  ARTED DEA+KFRR   +EKWM+ KK +   N+RDFT SSSSSSES+ TAG R
Sbjct: 145 TKQSVVTARTED-DEAVKFRRACMIEKWMDRKKVR---NIRDFTVSSSSSSESTSTAGRR 204

Query: 122 FSSSESEFLS----RPKPIKTT--TEIPKPKPKHENGFLKTKSKASKMYHDLKKVKQPIS 181
           +S+SESE LS    R KPIKTT  T+    K KHENG +KTKSKASK+YHDLKKVKQPIS
Sbjct: 205 YSASESELLSRALHRQKPIKTTPWTQTETQKAKHENGLVKTKSKASKIYHDLKKVKQPIS 264

Query: 182 PGARLANFLNSLFNGGTPKTKQKLSNSS---------VQSSKSLQGSTCSSTSSFSRSCL 241
           PG RLANFLNSLFNGG+PKTKQK+ N S         + +    QGSTCSS SSFSRSCL
Sbjct: 265 PGGRLANFLNSLFNGGSPKTKQKMPNPSTNPTSMDYDMPTKAKSQGSTCSSASSFSRSCL 324

Query: 242 SKTPSSRGNAAKRSVRFCPVSVIVDEDCRPCGHKILHKI-EPILKKPLK------TEQVK 301
           SKTPSSRGN  KRSVRFCPVS IVDEDC   GHK L K+ EPI+KK LK      TE+VK
Sbjct: 325 SKTPSSRGN-VKRSVRFCPVSEIVDEDCEAYGHKSLQKLEEPIMKKGLKSYGNFETEEVK 384

Query: 302 TEHDFE---DDDEDYDDEDDDGLSCSSSDLFELDNLSVIGIERYREELPVYETTNFKTNC 340
           TEH  +   ++  +Y+DE DD +SCSSSDLFELDNLSVIGIER+REELPVYETTNFKTNC
Sbjct: 385 TEHPRKIKRNEGFEYEDE-DDAVSCSSSDLFELDNLSVIGIERFREELPVYETTNFKTNC 444

BLAST of MS016140 vs. ExPASy TrEMBL
Match: A0A1S3CKK1 (protein BIG GRAIN 1-like B OS=Cucumis melo OX=3656 GN=LOC103501995 PE=3 SV=1)

HSP 1 Score: 390.2 bits (1001), Expect = 8.9e-105
Identity = 249/385 (64.68%), Postives = 283/385 (73.51%), Query Frame = 0

Query: 2   NLWDKPQP--LQREDRDHRRRLRRENPSFSSSLLDAIYRSIDESNGQGEEPQLILYRETT 61
           +LWD   P  LQ+      RR RR+NPSFSSSLLDAIYRSIDESN Q E+  LI Y   T
Sbjct: 2   DLWDHSDPHLLQQP-----RRSRRDNPSFSSSLLDAIYRSIDESNSQPED-HLIFYTHNT 61

Query: 62  MTKQSIAAARTEDADEALKFRRVEKWMEMKKKKVGSNVRDFTNSSSSSSESSCTAG-RFS 121
            TK +I  A      E+LKFR ++ W++ KKK    N+RDFT SSSSSSESS TAG RFS
Sbjct: 62  -TKHTILPATVSQDHESLKFRMIDTWIDKKKKL--RNIRDFTLSSSSSSESSSTAGRRFS 121

Query: 122 SSESEFLSR-----------PKPIKTT----TEIPKPKPKHENGFLKTKSKASKMYHDLK 181
           SSE+EFLSR           PKPIKT     TEI  P PKH+NGF+K+KSKASK+YHDLK
Sbjct: 122 SSETEFLSRALHRPTKLKPKPKPIKTNTWPQTEIITPNPKHDNGFVKSKSKASKIYHDLK 181

Query: 182 KVKQPISPGARLANFLNSLFNGGTPKTKQKLSNSSV------------QSSKSLQG--ST 241
           KVKQPISPGARLA+FLNSLFNGG+PK KQK+S+S+             + SKS QG  ST
Sbjct: 182 KVKQPISPGARLASFLNSLFNGGSPKAKQKISSSTCSINSTKFDYDMSRKSKSQQGSTST 241

Query: 242 CSSTSSFSRSCLSKTPSSRGNAAKRSVRFCPVSVIVDEDCRPCGHKILHKI-EPILKK-- 301
           CSS SSFSRSCLSKTPSSRGN  KRSVRFCPVSVIVDEDC+PCGHKILHK  EPI+KK  
Sbjct: 242 CSSASSFSRSCLSKTPSSRGN-IKRSVRFCPVSVIVDEDCQPCGHKILHKSEEPIMKKGN 301

Query: 302 ------PLKTEQVKTE------HDFEDDDEDYDDEDDDGLSCSSSDLFELDNLSVIGIER 340
                  L+ E+VKTE      ++ E++++D DD+DDD LSCSSSDLFELDNLSVIGIER
Sbjct: 302 LKKSNGNLEREKVKTEDMMKINYEEEEEEDDDDDDDDDALSCSSSDLFELDNLSVIGIER 361

BLAST of MS016140 vs. ExPASy TrEMBL
Match: A0A6J1IZW5 (protein BIG GRAIN 1-like B OS=Cucurbita maxima OX=3661 GN=LOC111479960 PE=3 SV=1)

HSP 1 Score: 388.3 bits (996), Expect = 3.4e-104
Identity = 252/380 (66.32%), Postives = 283/380 (74.47%), Query Frame = 0

Query: 2   NLWDKPQPLQREDRD-----HRRRLRRENPSFSSSLLDAIYRSIDESNGQGEEPQLILY- 61
           +LWD     QRED D      RRR RRENPSFSSSLLDAIYRSID+SN QGE+ QLI Y 
Sbjct: 2   DLWD--NSFQREDPDPHHHHQRRRSRRENPSFSSSLLDAIYRSIDDSNVQGED-QLIFYT 61

Query: 62  -RETTMTKQSIAAARTEDADEALKFRR---VEKWMEMKKKKVGSNVRDFTNSSSSSSESS 121
            + TT TKQSI+AA+TED  + L+F+R   ++ WM+ KK K    VRDFT S+SSSSESS
Sbjct: 62  QKTTTRTKQSISAAKTED-HQTLQFQRASMIKNWMDNKKIK---TVRDFTVSTSSSSESS 121

Query: 122 CTAG-RFSSSESEFLSR------------PKPIKTT----TEIPKPKPKHENGFLKTKSK 181
            TAG R SSSESEFLSR            PKPIKTT    TEI  P P HENGF+K+K +
Sbjct: 122 STAGRRLSSSESEFLSRTLHRPKPKPKPKPKPIKTTTWSQTEI-IPNPNHENGFVKSKFR 181

Query: 182 ASKMYHDLKKVKQPISPGARLANFLNSLFNGGTPKTKQKLSNS-----------SVQSSK 241
           ASK+YHDLKKVKQPISPG RLANFLNSLFNGG+PK KQK+SN+           + + SK
Sbjct: 182 ASKIYHDLKKVKQPISPGGRLANFLNSLFNGGSPKAKQKISNTCSNNSTNLDYDAPRKSK 241

Query: 242 SLQG--STCSSTSSFSRSCLSKTPSSRGNAAKRSVRFCPVSVIVDEDCRPCGHKILHKI- 301
           S QG  STCSS SSFSRSCL KTPSSRGN  KRSVRFCPVSVIVDEDCRPCGHK+LHK+ 
Sbjct: 242 SQQGSSSTCSSASSFSRSCLIKTPSSRGN-IKRSVRFCPVSVIVDEDCRPCGHKMLHKLE 301

Query: 302 EPILKK-PLKTEQVKTEHDFEDDDEDYDDEDDDGLSCSSSDLFELDNLSVIGIERYREEL 340
           EPI KK  L+TE++KTE          DDEDDD LSCSSSDLFELDNLS IG+ERYR+EL
Sbjct: 302 EPIGKKGKLETEEMKTEE---------DDEDDDRLSCSSSDLFELDNLSAIGMERYRDEL 361

BLAST of MS016140 vs. TAIR 10
Match: AT1G54200.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G13980.1); Has 1084 Blast hits to 581 proteins in 136 species: Archae - 0; Bacteria - 72; Metazoa - 212; Fungi - 78; Plants - 102; Viruses - 0; Other Eukaryotes - 620 (source: NCBI BLink). )

HSP 1 Score: 211.1 bits (536), Expect = 1.4e-54
Identity = 166/374 (44.39%), Postives = 211/374 (56.42%), Query Frame = 0

Query: 14  DRDHRRRLRRE--NPSFSSSLLDAIYRSIDESNGQGEEPQLILYRETTMTKQSIAAARTE 73
           D  HR R RR+  +PSFSS+LLD IYRSID+S+      +   ++    T+  ++A R +
Sbjct: 12  DHQHRLRNRRDHRHPSFSSTLLDQIYRSIDDSSTNSSSMRKTKHQNREDTR--VSANRRD 71

Query: 74  DADEALKFRRVEKWMEMKKKKVGSNVRDFTNSSSSSSESSCTAGRFSSSESEF------- 133
           D + +   + +E                F +SSSSSS+SS     FSSSES++       
Sbjct: 72  DFNRSKNLKTIEPVF-------------FKHSSSSSSDSS----GFSSSESDYFYRRSKS 131

Query: 134 ---LSRPKPIKTTTE---------IPKPKPKHENG-FLKTKSKASKMYHDLKKVKQPISP 193
              +S PKPI+TT E          P    K E+G FLKTKSKA K+Y DLKKVKQPISP
Sbjct: 132 SPAISHPKPIRTTVERFERSPQNHRPNSSNKQEHGSFLKTKSKALKIYSDLKKVKQPISP 191

Query: 194 GARLANFLNSLFNGGTPKTKQKLSNSSVQSSKSLQG-----STCSSTSSFSRSCLSKTPS 253
           G RLA FLNS+F G     K    N++V S+ +        +TCSS SSFSRSCLSKTPS
Sbjct: 192 GGRLATFLNSIFTGAGNTKKLNKINTTVTSTTAAAAAASSTTTCSSASSFSRSCLSKTPS 251

Query: 254 SRGNAAKRSVRFCPVSVIVDEDCRPCGHK--------------ILHKIE----------- 313
           S    +KRSVRFCPV+VI DED     +K              I H +E           
Sbjct: 252 S-SEKSKRSVRFCPVNVIFDEDSSKYNNKNNKVYGNNEREYESIRHTLENRVMEENRRVI 311

Query: 314 PILKKPLKTEQVKTEHDFE-DDDEDYDDEDDDGLSCSSSDLFELDNLSVIGIERYREELP 335
              K+ L++ Q K +   E   ++D +D+DDD LSC+SSDLFELDNLS IGI+RYREELP
Sbjct: 312 EAAKELLRSYQKKNKEVIEVSVEDDEEDDDDDALSCTSSDLFELDNLSAIGIDRYREELP 365

BLAST of MS016140 vs. TAIR 10
Match: AT3G13980.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G54200.1); Has 1485 Blast hits to 418 proteins in 98 species: Archae - 0; Bacteria - 6; Metazoa - 246; Fungi - 61; Plants - 107; Viruses - 6; Other Eukaryotes - 1059 (source: NCBI BLink). )

HSP 1 Score: 196.8 bits (499), Expect = 2.8e-50
Identity = 162/367 (44.14%), Postives = 206/367 (56.13%), Query Frame = 0

Query: 4   WDKPQPLQREDRDHRRRLRRENPSFSSSLLDAIYRSIDESNGQGEEPQLILYRETTMTKQ 63
           W+KP     +   H R     NPSFSS+LLD IYRSID+S+     P L   ++     Q
Sbjct: 5   WEKP-----KSSSHHR-----NPSFSSTLLDQIYRSIDDSS---PPPPLESIKKKKHHHQ 64

Query: 64  SIAAARTEDADEALKFRRVEKWMEMKKKKVGSNVRDFTNSSS------SSSESSCTAGRF 123
              A+  ED + +  + R     + ++ +  ++    +NSSS      SSSES    GR 
Sbjct: 65  QRNASLHEDREISPIYHRRSIAADFERSRRKTDFLRHSNSSSSDSSGFSSSESDSFHGRS 124

Query: 124 SSSESEFLS---RPKPIKTTT---EIPKPKPKHENGFLKTKSKASKMYHDLKKVKQPISP 183
            SS S   S   +PKPI+T++        KPK   GFL+TKSKA K+Y DLKKVKQPISP
Sbjct: 125 KSSASPPSSSRQQPKPIRTSSVDHSSAVQKPKELGGFLRTKSKALKIYSDLKKVKQPISP 184

Query: 184 GARLANFLNSLF-NGGTPKTKQKLSNSSVQSSKSLQGSTCSSTSSFSRSCLSKTPSSRGN 243
           G RLA FLNSLF N  T   K K + +     +    STCSS SSFSRSCLSKTPSS G 
Sbjct: 185 GGRLATFLNSLFTNAATNPKKHKKTTTVAVVEEPHSSSTCSSASSFSRSCLSKTPSSSGK 244

Query: 244 AAKRSVRFCPVSVIVDEDC-----------RPCGHKILHKIE------PILKKPLKTEQV 303
            +KRSVRFCPV+VI+DED            R   +    ++E         K  L+T   
Sbjct: 245 -SKRSVRFCPVNVILDEDSSFTMPYAYNNERLYDNNEAKRVEEHRRVIQAAKDLLRTYHN 304

Query: 304 KTEHDFEDDDEDYDDEDDDGLSCSSSDLFELDNLSVIGIERYREELPVYETTNF-KTNCA 340
           K +    + +   +D++DD  SC+SSDLFEL+NLS IGIERYREELPVYETT     N  
Sbjct: 305 KNKVTTTNINNVEEDDEDDAASCASSDLFELENLSAIGIERYREELPVYETTRLDNMNRV 357

BLAST of MS016140 vs. TAIR 10
Match: AT3G42800.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G54200.1); Has 853 Blast hits to 340 proteins in 98 species: Archae - 0; Bacteria - 94; Metazoa - 145; Fungi - 62; Plants - 95; Viruses - 0; Other Eukaryotes - 457 (source: NCBI BLink). )

HSP 1 Score: 126.7 bits (317), Expect = 3.5e-29
Identity = 121/344 (35.17%), Postives = 178/344 (51.74%), Query Frame = 0

Query: 22  RRENPSFSSSLLDAIYRSIDESNGQGEEPQLILYRETTMTKQSIAAARTEDADEALK--F 81
           R+  PSFSSS+LD++YRSIDES+G   + +  +    + +  S +  + +D    L+   
Sbjct: 6   RKRTPSFSSSVLDSVYRSIDESDGLQSDLKGSINENVSSSSSSPSPNKKDDKLTTLRRAI 65

Query: 82  RRVEKWMEMKKKKVGSNVRDFTNSSSSSSESSCTAGRFS--SSESEFLSRPKPIKTTTEI 141
              E W+  +     +N  D ++ SSS +ES  T  R    + + +     +     T +
Sbjct: 66  MDEEHWLYARSSTTTTNSSDSSSFSSSEAESYRTKRRLRKLAEQGKRSGDERQRTKRTVM 125

Query: 142 PKPKPKHENGFLKTKSKASKMYHDLKKVKQPISPGARLANFLNSLFNGGTPKTKQKLSNS 201
                         K KA K+  +LK+ KQP+SPGARL +FLNS+F     K K      
Sbjct: 126 DNDSRLFSKSDDDKKPKAVKIIEELKRSKQPVSPGARLTSFLNSIFQSNAKKVKL----C 185

Query: 202 SVQSSKSLQGSTCSSTSSFSRSCLSKTPSSRGNAAK--RSVRFCPVSVIVDEDCRPCGHK 261
           SV  +  ++ S  SS S FSR+  +KT ++  N  K  RS+RF PV V +D DCR    K
Sbjct: 186 SVGKTTDVKSS--SSKSCFSRT-RNKTDNNNNNCKKLERSIRFYPVRVTIDGDCRDYAQK 245

Query: 262 ILHKI-EPI--------LKKPLKTEQVKTE----------HDF-EDDDEDYDDEDDDGLS 321
            + ++ +PI        +K+ +KT    TE           DF   +  +  +E++D  S
Sbjct: 246 HITRVRKPIPEFTAKKSVKEEIKTNDHHTEFTCITRNIGLKDFVRSNKYEGKEEEEDAWS 305

Query: 322 CSSSDLFELDNLSVIGIERYREELPVYETTNFKTNCAIANGLVL 340
            SSSDLFELD+   IG+ RY +ELPVYETT+FKTN AIA  L+L
Sbjct: 306 HSSSDLFELDSYR-IGMGRYLKELPVYETTDFKTNQAIARSLLL 341

BLAST of MS016140 vs. TAIR 10
Match: AT5G12050.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G13980.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 100.5 bits (249), Expect = 2.7e-21
Identity = 129/375 (34.40%), Postives = 176/375 (46.93%), Query Frame = 0

Query: 23  RENPSFSSSLLDAIYRSIDESNGQGEEPQLILYRETTMTKQSIAAARTEDADEALKFRRV 82
           + NPSFSS+LLD IY SID    Q  +P  +    TT  KQSI      D     K  R 
Sbjct: 5   KRNPSFSSTLLDEIYNSIDPKT-QKTQP-YVGSVNTTTKKQSIVTRSVPDR----KIHRD 64

Query: 83  EKWMEMKKKKVGSNVRDFTNSSSSSSESSCTAGRFSSSESEFL---SRPKPIKT------ 142
             +  +      S+  D  +S  SSS++  T G+ ++S        S+ KP KT      
Sbjct: 65  RFFGSV------SSSSDSNSSIFSSSDTELTHGKKTTSSRPLCFGPSKTKPRKTEDKTLF 124

Query: 143 --------------TTEIPKPKPKHENGFLKTKSKAS-KMYHDLKKVKQPISPGARLANF 202
                          +++PK   +H+  +  T+++ S K   + KK K P SPG R+ NF
Sbjct: 125 HQNRATRVYDDYDYASDVPKFN-RHDENWENTRNRRSVKSSGNQKKPKTPASPGGRIVNF 184

Query: 203 LNSLFNGGTPKTK-----------------QKLSNSSVQSSKSLQGSTCSSTSSFSRSCL 262
           LNSLF+  + ++                  +K SN    S+     +TCSS SSFSRSC+
Sbjct: 185 LNSLFSNNSKQSNAVKSYPRKTSYDDSAYVRKTSNDYHSST-----TTCSSASSFSRSCM 244

Query: 263 SKTPSSRGNAAKRSVRFCPVSVIV-------DEDCRPCGH--KILHK---------IEPI 322
           +K         KRSVRF PV+VIV       +ED    G+  K + K         +E I
Sbjct: 245 NKGYEKSSGRIKRSVRFSPVNVIVPESFTSKEEDYFSNGNARKSVKKNVEDGGRRSVEEI 304

Query: 323 LKKPLK-------TEQVKTE--HDFEDDDEDYDDEDDDGLSCSSSDLFELDNLSVIGIER 326
            ++ L+          VKT    D+EDDDE  DD+DDD  S SSSDLFELD   ++G   
Sbjct: 305 AREFLRDYHKNHENSLVKTNGFEDYEDDDE--DDDDDDVASDSSSDLFELD---LVGNHH 356

BLAST of MS016140 vs. TAIR 10
Match: AT1G69160.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G13980.1); Has 173 Blast hits to 172 proteins in 54 species: Archae - 0; Bacteria - 0; Metazoa - 25; Fungi - 33; Plants - 84; Viruses - 2; Other Eukaryotes - 29 (source: NCBI BLink). )

HSP 1 Score: 42.7 bits (99), Expect = 6.7e-04
Identity = 66/209 (31.58%), Postives = 91/209 (43.54%), Query Frame = 0

Query: 166 KQPISPGARLANFLNSLFN-GGTPKTKQKLSNSS-------------------------- 225
           KQP SPG ++A+FLNSLF+  G+ K K K  + +                          
Sbjct: 114 KQPSSPGGKIASFLNSLFHQAGSKKNKSKSKSKTKPTDPEVEEEIPGGGWMRRRRRSSIS 173

Query: 226 --VQSSKSLQGSTCSSTSSFSRSCLS------KTPSSRGNAA----KRSVRFCPVSVIVD 285
               SS+S   +T ++ SS S+S +S      +TP    N      K+ + +   +  V 
Sbjct: 174 HFFSSSRSTSTTTTTTASSSSKSLISSSSSGFRTPPPYLNTPTKNYKQFLNYTSATKQVG 233

Query: 286 EDCRPCGHKILHKIEPILKKPLKT-EQVKTEHDFEDDDEDYDDE-------DDDGL-SCS 327
           E+         +K    L + LK  E +        DDED DD+       +DDG+ S S
Sbjct: 234 EE-----ETKTNKEYSWLDEKLKVMESLSENQRIWSDDEDIDDDRRIKREGEDDGMESDS 293