MS016137 (gene) Bitter gourd (TR) v1

Overview
NameMS016137
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionRING-type domain-containing protein
Locationscaffold9_2: 608691 .. 610028 (-)
RNA-Seq ExpressionMS016137
SyntenyMS016137
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCTAACGGGAAGTAACTCGAGAGAAGATATAATGGACCTCGATTTGAATCAAGAGCCTTTGGAGCAATCTTATGATTCAGTAATAGGGTTAGACTCCATACTGAATGACCTTGAAACTGCTCATGGACGCATTGAAGAACGCATCCGGCAACTTGAAGCTGTTACAACAAGGGCTACACGACGACAGAGATGGCGACATGCTCCGAATTTTATTGAACCTGCTGACTCTGCAGCAGCTGATACTCACGTTGAAAGACATGAGACTGGTGATGATAGTACTGCCCAGGAAAGAATAGTTCATTCAGGGAAAACTAGCAAAAAGGATGGCACTAAATTGATAGCCAAAGCATTGGGGATGGATACAGAGCCTAAGGCCACTGGGAACAAAATGGGAAGTTTTTTCGATTGTAATATATGCTTGGCTGTCGCAAAGGATCCGATATTGACATGTTGTGGTCACTTATTTTGTTGGTCTTGCTTTTATCAATTATCTTATGTTCATTCAAATGCCAAGGAATGCCCTGCATGTCAAGGTGAAGTTACAGATACAAGCATCATTCCTATCTACGGCCATGGGAATGCTAACCGTGCTCAGAAGTCAAAACGAAATGACTCTGGTTTGAAGGTTCCTCCCAGGCCTCGTGCTCAGAGGATTGAGAGTATGAGGCAGCAGATTTTATTGCGAGGGGCATCTTCTTCTCTCATTGAAGAAAGAATTCAGCAAATTAGCAACATGATTGGTGCAATGGGGGAGCAGTCTAGGGCACAGAATTTTGATGTTACACATGGAAGGACTGAGAGGACTAATCTGTTGGGCCGGAGGCGTCGTTCTTCCTACGCATTACCTGTTGTTGAGAACGAGAGCAATCAGCAAAGCCGATCCCTTCAAGTTTCTAGGTTACTATTGCAAGGTGCTGCCTCATTTTCATCTCTTTCATCTGCTTTAAATTCTGCAATGGACTCTGCTGAAAGATTAGTTGAGGACCTTGAGACATACATTCATAATCATAGTGCAAGTAGAAGCCGCCCACACTCTCTTCCTAATAATCCTAACAACGGAGACGCCTTGTCCGGTATTCCTGCGACTGTGCGATCAGATGGCATGACTCCAGATCCTGCTGGTGGAATCACCTTTATGGTTCCCCAATTCGAGTCATCTTCTAGAAGTATGGATATCGCGAGTAATATTGATCACCTAGGAAACCAGACCAGTACTGCTACTGAATTTAATCGAACAATACTCCCATCTTCATCGACCAGGCGGAGAAGTGAGTTACCACTACGGTCAGATATGGACAATGAAATTTTACAGGAGGCGAGGAGGAGAAGATTGGGA

mRNA sequence

ATGCTAACGGGAAGTAACTCGAGAGAAGATATAATGGACCTCGATTTGAATCAAGAGCCTTTGGAGCAATCTTATGATTCAGTAATAGGGTTAGACTCCATACTGAATGACCTTGAAACTGCTCATGGACGCATTGAAGAACGCATCCGGCAACTTGAAGCTGTTACAACAAGGGCTACACGACGACAGAGATGGCGACATGCTCCGAATTTTATTGAACCTGCTGACTCTGCAGCAGCTGATACTCACGTTGAAAGACATGAGACTGGTGATGATAGTACTGCCCAGGAAAGAATAGTTCATTCAGGGAAAACTAGCAAAAAGGATGGCACTAAATTGATAGCCAAAGCATTGGGGATGGATACAGAGCCTAAGGCCACTGGGAACAAAATGGGAAGTTTTTTCGATTGTAATATATGCTTGGCTGTCGCAAAGGATCCGATATTGACATGTTGTGGTCACTTATTTTGTTGGTCTTGCTTTTATCAATTATCTTATGTTCATTCAAATGCCAAGGAATGCCCTGCATGTCAAGGTGAAGTTACAGATACAAGCATCATTCCTATCTACGGCCATGGGAATGCTAACCGTGCTCAGAAGTCAAAACGAAATGACTCTGGTTTGAAGGTTCCTCCCAGGCCTCGTGCTCAGAGGATTGAGAGTATGAGGCAGCAGATTTTATTGCGAGGGGCATCTTCTTCTCTCATTGAAGAAAGAATTCAGCAAATTAGCAACATGATTGGTGCAATGGGGGAGCAGTCTAGGGCACAGAATTTTGATGTTACACATGGAAGGACTGAGAGGACTAATCTGTTGGGCCGGAGGCGTCGTTCTTCCTACGCATTACCTGTTGTTGAGAACGAGAGCAATCAGCAAAGCCGATCCCTTCAAGTTTCTAGGTTACTATTGCAAGGTGCTGCCTCATTTTCATCTCTTTCATCTGCTTTAAATTCTGCAATGGACTCTGCTGAAAGATTAGTTGAGGACCTTGAGACATACATTCATAATCATAGTGCAAGTAGAAGCCGCCCACACTCTCTTCCTAATAATCCTAACAACGGAGACGCCTTGTCCGGTATTCCTGCGACTGTGCGATCAGATGGCATGACTCCAGATCCTGCTGGTGGAATCACCTTTATGGTTCCCCAATTCGAGTCATCTTCTAGAAGTATGGATATCGCGAGTAATATTGATCACCTAGGAAACCAGACCAGTACTGCTACTGAATTTAATCGAACAATACTCCCATCTTCATCGACCAGGCGGAGAAGTGAGTTACCACTACGGTCAGATATGGACAATGAAATTTTACAGGAGGCGAGGAGGAGAAGATTGGGA

Coding sequence (CDS)

ATGCTAACGGGAAGTAACTCGAGAGAAGATATAATGGACCTCGATTTGAATCAAGAGCCTTTGGAGCAATCTTATGATTCAGTAATAGGGTTAGACTCCATACTGAATGACCTTGAAACTGCTCATGGACGCATTGAAGAACGCATCCGGCAACTTGAAGCTGTTACAACAAGGGCTACACGACGACAGAGATGGCGACATGCTCCGAATTTTATTGAACCTGCTGACTCTGCAGCAGCTGATACTCACGTTGAAAGACATGAGACTGGTGATGATAGTACTGCCCAGGAAAGAATAGTTCATTCAGGGAAAACTAGCAAAAAGGATGGCACTAAATTGATAGCCAAAGCATTGGGGATGGATACAGAGCCTAAGGCCACTGGGAACAAAATGGGAAGTTTTTTCGATTGTAATATATGCTTGGCTGTCGCAAAGGATCCGATATTGACATGTTGTGGTCACTTATTTTGTTGGTCTTGCTTTTATCAATTATCTTATGTTCATTCAAATGCCAAGGAATGCCCTGCATGTCAAGGTGAAGTTACAGATACAAGCATCATTCCTATCTACGGCCATGGGAATGCTAACCGTGCTCAGAAGTCAAAACGAAATGACTCTGGTTTGAAGGTTCCTCCCAGGCCTCGTGCTCAGAGGATTGAGAGTATGAGGCAGCAGATTTTATTGCGAGGGGCATCTTCTTCTCTCATTGAAGAAAGAATTCAGCAAATTAGCAACATGATTGGTGCAATGGGGGAGCAGTCTAGGGCACAGAATTTTGATGTTACACATGGAAGGACTGAGAGGACTAATCTGTTGGGCCGGAGGCGTCGTTCTTCCTACGCATTACCTGTTGTTGAGAACGAGAGCAATCAGCAAAGCCGATCCCTTCAAGTTTCTAGGTTACTATTGCAAGGTGCTGCCTCATTTTCATCTCTTTCATCTGCTTTAAATTCTGCAATGGACTCTGCTGAAAGATTAGTTGAGGACCTTGAGACATACATTCATAATCATAGTGCAAGTAGAAGCCGCCCACACTCTCTTCCTAATAATCCTAACAACGGAGACGCCTTGTCCGGTATTCCTGCGACTGTGCGATCAGATGGCATGACTCCAGATCCTGCTGGTGGAATCACCTTTATGGTTCCCCAATTCGAGTCATCTTCTAGAAGTATGGATATCGCGAGTAATATTGATCACCTAGGAAACCAGACCAGTACTGCTACTGAATTTAATCGAACAATACTCCCATCTTCATCGACCAGGCGGAGAAGTGAGTTACCACTACGGTCAGATATGGACAATGAAATTTTACAGGAGGCGAGGAGGAGAAGATTGGGA

Protein sequence

MLTGSNSREDIMDLDLNQEPLEQSYDSVIGLDSILNDLETAHGRIEERIRQLEAVTTRATRRQRWRHAPNFIEPADSAAADTHVERHETGDDSTAQERIVHSGKTSKKDGTKLIAKALGMDTEPKATGNKMGSFFDCNICLAVAKDPILTCCGHLFCWSCFYQLSYVHSNAKECPACQGEVTDTSIIPIYGHGNANRAQKSKRNDSGLKVPPRPRAQRIESMRQQILLRGASSSLIEERIQQISNMIGAMGEQSRAQNFDVTHGRTERTNLLGRRRRSSYALPVVENESNQQSRSLQVSRLLLQGAASFSSLSSALNSAMDSAERLVEDLETYIHNHSASRSRPHSLPNNPNNGDALSGIPATVRSDGMTPDPAGGITFMVPQFESSSRSMDIASNIDHLGNQTSTATEFNRTILPSSSTRRRSELPLRSDMDNEILQEARRRRLG
Homology
BLAST of MS016137 vs. NCBI nr
Match: XP_022152676.1 (uncharacterized protein LOC111020338 [Momordica charantia])

HSP 1 Score: 858.2 bits (2216), Expect = 3.1e-245
Identity = 444/446 (99.55%), Postives = 445/446 (99.78%), Query Frame = 0

Query: 1   MLTGSNSREDIMDLDLNQEPLEQSYDSVIGLDSILNDLETAHGRIEERIRQLEAVTTRAT 60
           MLTGSNSREDIMDLDLNQEPLEQSYDSVIGLDSILNDLETAHGRIEERIRQLEAVTTRAT
Sbjct: 1   MLTGSNSREDIMDLDLNQEPLEQSYDSVIGLDSILNDLETAHGRIEERIRQLEAVTTRAT 60

Query: 61  RRQRWRHAPNFIEPADSAAADTHVERHETGDDSTAQERIVHSGKTSKKDGTKLIAKALGM 120
           RRQRWRHAPNFIEPADSAAADTHVERHETGDDSTAQERIVHSGKTSKKDGTKLIAKALGM
Sbjct: 61  RRQRWRHAPNFIEPADSAAADTHVERHETGDDSTAQERIVHSGKTSKKDGTKLIAKALGM 120

Query: 121 DTEPKATGNKMGSFFDCNICLAVAKDPILTCCGHLFCWSCFYQLSYVHSNAKECPACQGE 180
           DTEPKATGNKMGSFFDCNICLAVAKDPILTCCGHLFCWSCFYQLSYVHSNAKECPACQGE
Sbjct: 121 DTEPKATGNKMGSFFDCNICLAVAKDPILTCCGHLFCWSCFYQLSYVHSNAKECPACQGE 180

Query: 181 VTDTSIIPIYGHGNANRAQKSKRNDSGLKVPPRPRAQRIESMRQQILLRGASSSLIEERI 240
           VTDTSIIPIYGHGNANRAQKSKRNDSGLKVPPRPRAQRIESMRQQILLRGASSSLIEERI
Sbjct: 181 VTDTSIIPIYGHGNANRAQKSKRNDSGLKVPPRPRAQRIESMRQQILLRGASSSLIEERI 240

Query: 241 QQISNMIGAMGEQSRAQNFDVTHGRTERTNLLGRRRRSSYALPVVENESNQQSRSLQVSR 300
           QQISNMIGAMGEQSRAQNFDVTHGRTERTNLLGRRRRSSY LPVVENESNQQSRSLQVSR
Sbjct: 241 QQISNMIGAMGEQSRAQNFDVTHGRTERTNLLGRRRRSSYVLPVVENESNQQSRSLQVSR 300

Query: 301 LLLQGAASFSSLSSALNSAMDSAERLVEDLETYIHNHSASRSRPHSLPNNPNNGDALSGI 360
           LLLQGAASFSSLSSALNSAMDSAERLVEDLETYIHNHSASRSRPHSLPNNPNNGDALSGI
Sbjct: 301 LLLQGAASFSSLSSALNSAMDSAERLVEDLETYIHNHSASRSRPHSLPNNPNNGDALSGI 360

Query: 361 PATVRSDGMTPDPAGGITFMVPQFESSSRSMDIASNIDHLGNQTSTATEFNRTILPSSST 420
           PATVRSDGMTPDPAGGITFMVPQ+ESSSRSMDIASNIDHLGNQTSTATEFNRTILPSSST
Sbjct: 361 PATVRSDGMTPDPAGGITFMVPQYESSSRSMDIASNIDHLGNQTSTATEFNRTILPSSST 420

Query: 421 RRRSELPLRSDMDNEILQEARRRRLG 447
           RRRSELPLRSDMDNEILQEARRRRLG
Sbjct: 421 RRRSELPLRSDMDNEILQEARRRRLG 446

BLAST of MS016137 vs. NCBI nr
Match: XP_023527862.1 (uncharacterized protein LOC111790954 [Cucurbita pepo subsp. pepo] >XP_023527863.1 uncharacterized protein LOC111790954 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 743.8 bits (1919), Expect = 8.6e-211
Identity = 384/443 (86.68%), Postives = 406/443 (91.65%), Query Frame = 0

Query: 4   GSNSREDIMDLDLNQEPLEQSYDSVIGLDSILNDLETAHGRIEERIRQLEAVTTRATRRQ 63
           G+NSREDIMDLDLNQEPL+QSYDSV+GLD+ILNDLE AHGRIEERIRQLEAVTTRATRRQ
Sbjct: 2   GNNSREDIMDLDLNQEPLDQSYDSVVGLDTILNDLENAHGRIEERIRQLEAVTTRATRRQ 61

Query: 64  RWRHAPNFIEPADSAAADTHVERHETGDDSTAQERIVHSGKTSKKDGTKLIAKALGMDTE 123
           RWRHAP   EPAD+AAA  H+ERH+TGDDS AQER+ +S KTSKKDG+ L+AKALGMD+E
Sbjct: 62  RWRHAPTVTEPADTAAAYAHLERHDTGDDSAAQERVANSTKTSKKDGSHLVAKALGMDSE 121

Query: 124 PKATGNKMGSFFDCNICLAVAKDPILTCCGHLFCWSCFYQLSYVHSNAKECPACQGEVTD 183
            K TGNKMGSFFDCNICL VAKDPILTCCGHLFCWSCFYQLSYVHSNAKECP CQGEVTD
Sbjct: 122 LKPTGNKMGSFFDCNICLDVAKDPILTCCGHLFCWSCFYQLSYVHSNAKECPECQGEVTD 181

Query: 184 TSIIPIYGHGNANRAQKSKRNDSGLKVPPRPRAQRIESMRQQILLRGASSSLIEERIQQI 243
           TSIIPIYGHGN NRAQKSK NDSGLKVPPRPRAQRIESMRQQIL +GASSSLIEERIQQI
Sbjct: 182 TSIIPIYGHGNGNRAQKSKPNDSGLKVPPRPRAQRIESMRQQILFQGASSSLIEERIQQI 241

Query: 244 SNMIGAMGEQSRAQNFDVTHGRTERTNLLGRRRRSSYALPVVENESNQQSRSLQVSRLLL 303
           SNMIGAMGEQSR+QN D+ HGR ERTNLLGRRRR+S  LPV ENESNQQSRSLQVSRLLL
Sbjct: 242 SNMIGAMGEQSRSQNSDIMHGRIERTNLLGRRRRASQVLPVTENESNQQSRSLQVSRLLL 301

Query: 304 QGAASFSSLSSALNSAMDSAERLVEDLETYIHNHSASRSRPHSLPNNPNNGDALSGIPAT 363
           QGAASFSSLSSALNSAMDSAERLVEDLETYIHNH ASRSRPHSLPNNPNNGD LSGI AT
Sbjct: 302 QGAASFSSLSSALNSAMDSAERLVEDLETYIHNHGASRSRPHSLPNNPNNGDPLSGIAAT 361

Query: 364 VRSDGMTPDPAGGITFMVPQFESSSRSMDIASNIDHLGNQTSTATEFNRTILPSSSTRRR 423
           VRSDG+ PDP GGITF+VPQFESSSRSMDIA+NI  L NQTS+ TEF+R ILPS STRRR
Sbjct: 362 VRSDGIPPDPVGGITFLVPQFESSSRSMDIATNIARLENQTSSGTEFDRIILPSPSTRRR 421

Query: 424 SELPLRSDMDNEILQEARRRRLG 447
           SELP RSD+DNE LQE RRRRLG
Sbjct: 422 SELPRRSDLDNENLQERRRRRLG 444

BLAST of MS016137 vs. NCBI nr
Match: XP_022934926.1 (uncharacterized protein LOC111441954 [Cucurbita moschata] >XP_022934927.1 uncharacterized protein LOC111441954 [Cucurbita moschata] >KAG7018104.1 E3 ubiquitin-protein ligase RNF5, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 743.4 bits (1918), Expect = 1.1e-210
Identity = 384/443 (86.68%), Postives = 406/443 (91.65%), Query Frame = 0

Query: 4   GSNSREDIMDLDLNQEPLEQSYDSVIGLDSILNDLETAHGRIEERIRQLEAVTTRATRRQ 63
           G+NSREDIMDLDLNQEPL+QSYDSV+GLD+ILNDLE AHGRIEERIRQLEAVTTRATRRQ
Sbjct: 2   GNNSREDIMDLDLNQEPLDQSYDSVVGLDTILNDLENAHGRIEERIRQLEAVTTRATRRQ 61

Query: 64  RWRHAPNFIEPADSAAADTHVERHETGDDSTAQERIVHSGKTSKKDGTKLIAKALGMDTE 123
           RWRHAP   EPAD+AAA  H+ERH+TGDDS AQER+ +S KTSKKDG+ L+AKALGMD E
Sbjct: 62  RWRHAPTVTEPADTAAAYAHLERHDTGDDSAAQERVANSTKTSKKDGSHLVAKALGMDPE 121

Query: 124 PKATGNKMGSFFDCNICLAVAKDPILTCCGHLFCWSCFYQLSYVHSNAKECPACQGEVTD 183
            K TGNKMGSFFDCNICL VAKDPILTCCGHLFCWSCFYQLSYVHSNAKECP CQGEVTD
Sbjct: 122 LKPTGNKMGSFFDCNICLDVAKDPILTCCGHLFCWSCFYQLSYVHSNAKECPECQGEVTD 181

Query: 184 TSIIPIYGHGNANRAQKSKRNDSGLKVPPRPRAQRIESMRQQILLRGASSSLIEERIQQI 243
           TSIIPIYGHGN NRAQKSK NDSGLKVPPRPRAQRIESMRQQIL +GASSSLIEERIQQI
Sbjct: 182 TSIIPIYGHGNGNRAQKSKPNDSGLKVPPRPRAQRIESMRQQILFQGASSSLIEERIQQI 241

Query: 244 SNMIGAMGEQSRAQNFDVTHGRTERTNLLGRRRRSSYALPVVENESNQQSRSLQVSRLLL 303
           SNMIGAMGEQSR+QN D+ HGR ERTNLLGRRRR+S  LPV ENESNQQSRSLQVSRLLL
Sbjct: 242 SNMIGAMGEQSRSQNSDIMHGRIERTNLLGRRRRASQVLPVTENESNQQSRSLQVSRLLL 301

Query: 304 QGAASFSSLSSALNSAMDSAERLVEDLETYIHNHSASRSRPHSLPNNPNNGDALSGIPAT 363
           QGAASFSSLSSALNSAMDSAERLVEDLETYIHNH ASRSRPHSLPNNPNNGD LSGI AT
Sbjct: 302 QGAASFSSLSSALNSAMDSAERLVEDLETYIHNHGASRSRPHSLPNNPNNGDPLSGIAAT 361

Query: 364 VRSDGMTPDPAGGITFMVPQFESSSRSMDIASNIDHLGNQTSTATEFNRTILPSSSTRRR 423
           VRSDG+ PDP GGITF+VPQFESSSRSMDIA+NI  L NQTS++TEF+R ILPS STRRR
Sbjct: 362 VRSDGIPPDPVGGITFLVPQFESSSRSMDIATNIARLENQTSSSTEFDRIILPSPSTRRR 421

Query: 424 SELPLRSDMDNEILQEARRRRLG 447
           SELP RSD+DNE LQE RRRRLG
Sbjct: 422 SELPRRSDLDNENLQERRRRRLG 444

BLAST of MS016137 vs. NCBI nr
Match: XP_022983257.1 (uncharacterized protein LOC111481891 [Cucurbita maxima] >XP_022983258.1 uncharacterized protein LOC111481891 [Cucurbita maxima])

HSP 1 Score: 740.0 bits (1909), Expect = 1.2e-209
Identity = 383/446 (85.87%), Postives = 404/446 (90.58%), Query Frame = 0

Query: 1   MLTGSNSREDIMDLDLNQEPLEQSYDSVIGLDSILNDLETAHGRIEERIRQLEAVTTRAT 60
           ML G+NSREDIMDLDLNQEPL+QSYDSV+GLD+ILNDLE AHGRIEERIRQLEAVTTRAT
Sbjct: 1   MLMGNNSREDIMDLDLNQEPLDQSYDSVVGLDTILNDLENAHGRIEERIRQLEAVTTRAT 60

Query: 61  RRQRWRHAPNFIEPADSAAADTHVERHETGDDSTAQERIVHSGKTSKKDGTKLIAKALGM 120
           RRQRWRHAP   E AD+AAA  H+ERH+TGDDS AQER+ +S K SKKDG+ L+AKALGM
Sbjct: 61  RRQRWRHAPTVTESADTAAAYAHLERHDTGDDSAAQERVANSTKASKKDGSHLVAKALGM 120

Query: 121 DTEPKATGNKMGSFFDCNICLAVAKDPILTCCGHLFCWSCFYQLSYVHSNAKECPACQGE 180
           D E K TGNKMGSF+DCNICL VAKDPILTCCGHLFCWSCFYQLSYVHSNAKECP CQGE
Sbjct: 121 DPELKPTGNKMGSFYDCNICLDVAKDPILTCCGHLFCWSCFYQLSYVHSNAKECPECQGE 180

Query: 181 VTDTSIIPIYGHGNANRAQKSKRNDSGLKVPPRPRAQRIESMRQQILLRGASSSLIEERI 240
           VTDTSIIPIYGHGN NRAQKSK NDSGLKVPPRPRAQRIESMRQQIL +GASSSLIEERI
Sbjct: 181 VTDTSIIPIYGHGNGNRAQKSKPNDSGLKVPPRPRAQRIESMRQQILFQGASSSLIEERI 240

Query: 241 QQISNMIGAMGEQSRAQNFDVTHGRTERTNLLGRRRRSSYALPVVENESNQQSRSLQVSR 300
           QQISNMIGAMGEQSR+QN D+ HGR ERTNLLGRRRR S  LPV ENESNQQSRSLQVSR
Sbjct: 241 QQISNMIGAMGEQSRSQNSDIMHGRIERTNLLGRRRRPSQVLPVTENESNQQSRSLQVSR 300

Query: 301 LLLQGAASFSSLSSALNSAMDSAERLVEDLETYIHNHSASRSRPHSLPNNPNNGDALSGI 360
           LLLQGAASFSSLSSALNSAMDSAERLVEDLETYIHNH ASRSRPHSLPNNPNNGD LSGI
Sbjct: 301 LLLQGAASFSSLSSALNSAMDSAERLVEDLETYIHNHGASRSRPHSLPNNPNNGDPLSGI 360

Query: 361 PATVRSDGMTPDPAGGITFMVPQFESSSRSMDIASNIDHLGNQTSTATEFNRTILPSSST 420
            ATVRSDG+ PDP GGITF+VPQFESSSRSMDIA+NI  L NQTS+ TEF+R ILPS ST
Sbjct: 361 AATVRSDGIPPDPVGGITFLVPQFESSSRSMDIATNISRLENQTSSGTEFDRIILPSPST 420

Query: 421 RRRSELPLRSDMDNEILQEARRRRLG 447
           RRRSELP RSD+DNE LQE RRRRLG
Sbjct: 421 RRRSELPRRSDLDNENLQERRRRRLG 446

BLAST of MS016137 vs. NCBI nr
Match: XP_038905049.1 (uncharacterized protein LOC120091205 [Benincasa hispida])

HSP 1 Score: 735.3 bits (1897), Expect = 3.0e-208
Identity = 381/447 (85.23%), Postives = 412/447 (92.17%), Query Frame = 0

Query: 1   MLTGSNSREDIMDLDLNQEPLEQSYDSVIGLDSILNDLETAHGRIEERIRQLEAVTTRAT 60
           ML G+N+ EDIMDLDLNQEPL+QSYDSVIGLD+ILNDLETAHGRIEERIRQLEAVTTRAT
Sbjct: 1   MLMGNNAGEDIMDLDLNQEPLDQSYDSVIGLDTILNDLETAHGRIEERIRQLEAVTTRAT 60

Query: 61  RRQRWRHAPNFIEPADSAAADTHVERHETGDDS-TAQERIVHSGKTSKKDGTKLIAKALG 120
           RRQRWRHAP   EPAD+AAA  H+ERH+TGDDS  AQER +HSGKTSK++G+ L+AKALG
Sbjct: 61  RRQRWRHAPTVTEPADTAAAYVHLERHDTGDDSAAAQERTLHSGKTSKQNGSHLVAKALG 120

Query: 121 MDTEPKATGNKMGSFFDCNICLAVAKDPILTCCGHLFCWSCFYQLSYVHSNAKECPACQG 180
           MD EP+ TGNKMGSF+DCNICL VAKDPILTCCGHLFCWSCFYQLSYVHSNAKECP CQG
Sbjct: 121 MDPEPRTTGNKMGSFYDCNICLDVAKDPILTCCGHLFCWSCFYQLSYVHSNAKECPECQG 180

Query: 181 EVTDTSIIPIYGHGNANRAQKSKRNDSGLKVPPRPRAQRIESMRQQILLRGASSSLIEER 240
           EVTDTSIIPIYGHGN NRA KSK NDSGLKVPPRPRAQRIES RQQIL RGASSSLIEER
Sbjct: 181 EVTDTSIIPIYGHGNGNRAHKSKPNDSGLKVPPRPRAQRIESTRQQILFRGASSSLIEER 240

Query: 241 IQQISNMIGAMGEQSRAQNFDVTHGRTERTNLLGRRRRSSYALPVVENESNQQSRSLQVS 300
           IQQISNMIGAMGEQSR+QNFDV+HGR ERTNLLGRRRR+S  LPV ENESNQQSRSLQVS
Sbjct: 241 IQQISNMIGAMGEQSRSQNFDVSHGRIERTNLLGRRRRASQVLPVPENESNQQSRSLQVS 300

Query: 301 RLLLQGAASFSSLSSALNSAMDSAERLVEDLETYIHNHSASRSRPHSLPNNPNNGDALSG 360
           RLLLQGAASFSSLSSALNSAMDSAERLVEDLETYIHNHSA+RSRPHS+PNNPNNGD LSG
Sbjct: 301 RLLLQGAASFSSLSSALNSAMDSAERLVEDLETYIHNHSATRSRPHSIPNNPNNGDPLSG 360

Query: 361 IPATVRSDGMTPDPAGGITFMVPQFESSSRSMDIASNIDHLGNQTSTATEFNRTILPSSS 420
           I ATV+SDG+ PDP GGITF+VPQFESSSRSMDIA++++   NQT++A+EF+R ILPS S
Sbjct: 361 IAATVQSDGIIPDPVGGITFLVPQFESSSRSMDIATSLE---NQTNSASEFDRIILPSPS 420

Query: 421 TRRRSELPLRSDMDNEILQEARRRRLG 447
           TRRRSELP RSD+DNEILQE RRRRLG
Sbjct: 421 TRRRSELPRRSDIDNEILQERRRRRLG 444

BLAST of MS016137 vs. ExPASy Swiss-Prot
Match: Q09463 (E3 ubiquitin ligase rnf-5 OS=Caenorhabditis elegans OX=6239 GN=rnf-5 PE=1 SV=1)

HSP 1 Score: 89.0 bits (219), Expect = 1.5e-16
Identity = 42/99 (42.42%), Postives = 56/99 (56.57%), Query Frame = 0

Query: 123 EPKATGNKMGSF-FDCNICLAVAKDPILTCCGHLFCWSCFYQLSYVHSNAKECPACQGEV 182
           EP ++ NK  S  F+CNICL  AKD +++ CGHLFCW C  Q      N + CP C+  +
Sbjct: 11  EPTSSSNKDESARFECNICLDAAKDAVVSLCGHLFCWPCLSQWLDTRPNNQVCPVCKSAI 70

Query: 183 TDTSIIPIYGHGNANRAQKSKRNDSGLKVPPRPRAQRIE 221
               ++PIYG G  +       +D   KVPPRP+ QR E
Sbjct: 71  DGNKVVPIYGRGGDS-------SDPRKKVPPRPKGQRSE 102

BLAST of MS016137 vs. ExPASy Swiss-Prot
Match: Q6PC78 (E3 ubiquitin-protein ligase RNF185 OS=Danio rerio OX=7955 GN=rnf185 PE=2 SV=1)

HSP 1 Score: 88.2 bits (217), Expect = 2.6e-16
Identity = 40/119 (33.61%), Postives = 59/119 (49.58%), Query Frame = 0

Query: 102 SGKTSKKDGTKLIAKALGMDTEPKATGNKMGSFFDCNICLAVAKDPILTCCGHLFCWSCF 161
           + ++S    +     A G        G    S F+CNICL  +KD +++ CGHLFCW C 
Sbjct: 6   ASESSSSSSSSSAGAANGQSAGESGGGGAQDSTFECNICLDTSKDAVISLCGHLFCWPCL 65

Query: 162 YQLSYVHSNAKECPACQGEVTDTSIIPIYGHGNANRAQKSKRNDSGLKVPPRPRAQRIE 221
           +Q      N + CP C+  ++   +IP+YG G+        + D   K PPRP+ QR E
Sbjct: 66  HQWLETRPNRQVCPVCKAGISRDKVIPLYGRGSTG------QQDPREKTPPRPQGQRPE 118

BLAST of MS016137 vs. ExPASy Swiss-Prot
Match: Q91YT2 (E3 ubiquitin-protein ligase RNF185 OS=Mus musculus OX=10090 GN=Rnf185 PE=2 SV=1)

HSP 1 Score: 87.8 bits (216), Expect = 3.3e-16
Identity = 42/108 (38.89%), Postives = 57/108 (52.78%), Query Frame = 0

Query: 115 AKALGMDTEPKATGNKMG--SFFDCNICLAVAKDPILTCCGHLFCWSCFYQLSYVHSNAK 174
           + A G       TG   G  S F+CNICL  AKD +++ CGHLFCW C +Q      N +
Sbjct: 15  SNAGGPSGSSNGTGESGGQDSTFECNICLDTAKDAVISLCGHLFCWPCLHQWLETRPNRQ 74

Query: 175 ECPACQGEVTDTSIIPIYGHGNANRAQKSKRNDSGLKVPPRPRAQRIE 221
            CP C+  ++   +IP+YG G+        + D   K PPRP+ QR E
Sbjct: 75  VCPVCKAGISRDKVIPLYGRGSTG------QQDPREKTPPRPQGQRPE 116

BLAST of MS016137 vs. ExPASy Swiss-Prot
Match: Q568Y3 (E3 ubiquitin-protein ligase RNF185 OS=Rattus norvegicus OX=10116 GN=Rnf185 PE=2 SV=1)

HSP 1 Score: 87.8 bits (216), Expect = 3.3e-16
Identity = 42/108 (38.89%), Postives = 57/108 (52.78%), Query Frame = 0

Query: 115 AKALGMDTEPKATGNKMG--SFFDCNICLAVAKDPILTCCGHLFCWSCFYQLSYVHSNAK 174
           + A G       TG   G  S F+CNICL  AKD +++ CGHLFCW C +Q      N +
Sbjct: 15  SSAGGPSGSSNGTGESGGQDSTFECNICLDTAKDAVISLCGHLFCWPCLHQWLETRPNRQ 74

Query: 175 ECPACQGEVTDTSIIPIYGHGNANRAQKSKRNDSGLKVPPRPRAQRIE 221
            CP C+  ++   +IP+YG G+        + D   K PPRP+ QR E
Sbjct: 75  VCPVCKAGISRDKVIPLYGRGSTG------QQDPREKTPPRPQGQRPE 116

BLAST of MS016137 vs. ExPASy Swiss-Prot
Match: Q96GF1 (E3 ubiquitin-protein ligase RNF185 OS=Homo sapiens OX=9606 GN=RNF185 PE=1 SV=1)

HSP 1 Score: 86.7 bits (213), Expect = 7.5e-16
Identity = 37/88 (42.05%), Postives = 51/88 (57.95%), Query Frame = 0

Query: 133 SFFDCNICLAVAKDPILTCCGHLFCWSCFYQLSYVHSNAKECPACQGEVTDTSIIPIYGH 192
           S F+CNICL  AKD +++ CGHLFCW C +Q      N + CP C+  ++   +IP+YG 
Sbjct: 35  STFECNICLDTAKDAVISLCGHLFCWPCLHQWLETRPNRQVCPVCKAGISRDKVIPLYGR 94

Query: 193 GNANRAQKSKRNDSGLKVPPRPRAQRIE 221
           G+        + D   K PPRP+ QR E
Sbjct: 95  GSTG------QQDPREKTPPRPQGQRPE 116

BLAST of MS016137 vs. ExPASy TrEMBL
Match: A0A6J1DIG1 (uncharacterized protein LOC111020338 OS=Momordica charantia OX=3673 GN=LOC111020338 PE=4 SV=1)

HSP 1 Score: 858.2 bits (2216), Expect = 1.5e-245
Identity = 444/446 (99.55%), Postives = 445/446 (99.78%), Query Frame = 0

Query: 1   MLTGSNSREDIMDLDLNQEPLEQSYDSVIGLDSILNDLETAHGRIEERIRQLEAVTTRAT 60
           MLTGSNSREDIMDLDLNQEPLEQSYDSVIGLDSILNDLETAHGRIEERIRQLEAVTTRAT
Sbjct: 1   MLTGSNSREDIMDLDLNQEPLEQSYDSVIGLDSILNDLETAHGRIEERIRQLEAVTTRAT 60

Query: 61  RRQRWRHAPNFIEPADSAAADTHVERHETGDDSTAQERIVHSGKTSKKDGTKLIAKALGM 120
           RRQRWRHAPNFIEPADSAAADTHVERHETGDDSTAQERIVHSGKTSKKDGTKLIAKALGM
Sbjct: 61  RRQRWRHAPNFIEPADSAAADTHVERHETGDDSTAQERIVHSGKTSKKDGTKLIAKALGM 120

Query: 121 DTEPKATGNKMGSFFDCNICLAVAKDPILTCCGHLFCWSCFYQLSYVHSNAKECPACQGE 180
           DTEPKATGNKMGSFFDCNICLAVAKDPILTCCGHLFCWSCFYQLSYVHSNAKECPACQGE
Sbjct: 121 DTEPKATGNKMGSFFDCNICLAVAKDPILTCCGHLFCWSCFYQLSYVHSNAKECPACQGE 180

Query: 181 VTDTSIIPIYGHGNANRAQKSKRNDSGLKVPPRPRAQRIESMRQQILLRGASSSLIEERI 240
           VTDTSIIPIYGHGNANRAQKSKRNDSGLKVPPRPRAQRIESMRQQILLRGASSSLIEERI
Sbjct: 181 VTDTSIIPIYGHGNANRAQKSKRNDSGLKVPPRPRAQRIESMRQQILLRGASSSLIEERI 240

Query: 241 QQISNMIGAMGEQSRAQNFDVTHGRTERTNLLGRRRRSSYALPVVENESNQQSRSLQVSR 300
           QQISNMIGAMGEQSRAQNFDVTHGRTERTNLLGRRRRSSY LPVVENESNQQSRSLQVSR
Sbjct: 241 QQISNMIGAMGEQSRAQNFDVTHGRTERTNLLGRRRRSSYVLPVVENESNQQSRSLQVSR 300

Query: 301 LLLQGAASFSSLSSALNSAMDSAERLVEDLETYIHNHSASRSRPHSLPNNPNNGDALSGI 360
           LLLQGAASFSSLSSALNSAMDSAERLVEDLETYIHNHSASRSRPHSLPNNPNNGDALSGI
Sbjct: 301 LLLQGAASFSSLSSALNSAMDSAERLVEDLETYIHNHSASRSRPHSLPNNPNNGDALSGI 360

Query: 361 PATVRSDGMTPDPAGGITFMVPQFESSSRSMDIASNIDHLGNQTSTATEFNRTILPSSST 420
           PATVRSDGMTPDPAGGITFMVPQ+ESSSRSMDIASNIDHLGNQTSTATEFNRTILPSSST
Sbjct: 361 PATVRSDGMTPDPAGGITFMVPQYESSSRSMDIASNIDHLGNQTSTATEFNRTILPSSST 420

Query: 421 RRRSELPLRSDMDNEILQEARRRRLG 447
           RRRSELPLRSDMDNEILQEARRRRLG
Sbjct: 421 RRRSELPLRSDMDNEILQEARRRRLG 446

BLAST of MS016137 vs. ExPASy TrEMBL
Match: A0A6J1F461 (uncharacterized protein LOC111441954 OS=Cucurbita moschata OX=3662 GN=LOC111441954 PE=4 SV=1)

HSP 1 Score: 743.4 bits (1918), Expect = 5.4e-211
Identity = 384/443 (86.68%), Postives = 406/443 (91.65%), Query Frame = 0

Query: 4   GSNSREDIMDLDLNQEPLEQSYDSVIGLDSILNDLETAHGRIEERIRQLEAVTTRATRRQ 63
           G+NSREDIMDLDLNQEPL+QSYDSV+GLD+ILNDLE AHGRIEERIRQLEAVTTRATRRQ
Sbjct: 2   GNNSREDIMDLDLNQEPLDQSYDSVVGLDTILNDLENAHGRIEERIRQLEAVTTRATRRQ 61

Query: 64  RWRHAPNFIEPADSAAADTHVERHETGDDSTAQERIVHSGKTSKKDGTKLIAKALGMDTE 123
           RWRHAP   EPAD+AAA  H+ERH+TGDDS AQER+ +S KTSKKDG+ L+AKALGMD E
Sbjct: 62  RWRHAPTVTEPADTAAAYAHLERHDTGDDSAAQERVANSTKTSKKDGSHLVAKALGMDPE 121

Query: 124 PKATGNKMGSFFDCNICLAVAKDPILTCCGHLFCWSCFYQLSYVHSNAKECPACQGEVTD 183
            K TGNKMGSFFDCNICL VAKDPILTCCGHLFCWSCFYQLSYVHSNAKECP CQGEVTD
Sbjct: 122 LKPTGNKMGSFFDCNICLDVAKDPILTCCGHLFCWSCFYQLSYVHSNAKECPECQGEVTD 181

Query: 184 TSIIPIYGHGNANRAQKSKRNDSGLKVPPRPRAQRIESMRQQILLRGASSSLIEERIQQI 243
           TSIIPIYGHGN NRAQKSK NDSGLKVPPRPRAQRIESMRQQIL +GASSSLIEERIQQI
Sbjct: 182 TSIIPIYGHGNGNRAQKSKPNDSGLKVPPRPRAQRIESMRQQILFQGASSSLIEERIQQI 241

Query: 244 SNMIGAMGEQSRAQNFDVTHGRTERTNLLGRRRRSSYALPVVENESNQQSRSLQVSRLLL 303
           SNMIGAMGEQSR+QN D+ HGR ERTNLLGRRRR+S  LPV ENESNQQSRSLQVSRLLL
Sbjct: 242 SNMIGAMGEQSRSQNSDIMHGRIERTNLLGRRRRASQVLPVTENESNQQSRSLQVSRLLL 301

Query: 304 QGAASFSSLSSALNSAMDSAERLVEDLETYIHNHSASRSRPHSLPNNPNNGDALSGIPAT 363
           QGAASFSSLSSALNSAMDSAERLVEDLETYIHNH ASRSRPHSLPNNPNNGD LSGI AT
Sbjct: 302 QGAASFSSLSSALNSAMDSAERLVEDLETYIHNHGASRSRPHSLPNNPNNGDPLSGIAAT 361

Query: 364 VRSDGMTPDPAGGITFMVPQFESSSRSMDIASNIDHLGNQTSTATEFNRTILPSSSTRRR 423
           VRSDG+ PDP GGITF+VPQFESSSRSMDIA+NI  L NQTS++TEF+R ILPS STRRR
Sbjct: 362 VRSDGIPPDPVGGITFLVPQFESSSRSMDIATNIARLENQTSSSTEFDRIILPSPSTRRR 421

Query: 424 SELPLRSDMDNEILQEARRRRLG 447
           SELP RSD+DNE LQE RRRRLG
Sbjct: 422 SELPRRSDLDNENLQERRRRRLG 444

BLAST of MS016137 vs. ExPASy TrEMBL
Match: A0A6J1J795 (uncharacterized protein LOC111481891 OS=Cucurbita maxima OX=3661 GN=LOC111481891 PE=4 SV=1)

HSP 1 Score: 740.0 bits (1909), Expect = 6.0e-210
Identity = 383/446 (85.87%), Postives = 404/446 (90.58%), Query Frame = 0

Query: 1   MLTGSNSREDIMDLDLNQEPLEQSYDSVIGLDSILNDLETAHGRIEERIRQLEAVTTRAT 60
           ML G+NSREDIMDLDLNQEPL+QSYDSV+GLD+ILNDLE AHGRIEERIRQLEAVTTRAT
Sbjct: 1   MLMGNNSREDIMDLDLNQEPLDQSYDSVVGLDTILNDLENAHGRIEERIRQLEAVTTRAT 60

Query: 61  RRQRWRHAPNFIEPADSAAADTHVERHETGDDSTAQERIVHSGKTSKKDGTKLIAKALGM 120
           RRQRWRHAP   E AD+AAA  H+ERH+TGDDS AQER+ +S K SKKDG+ L+AKALGM
Sbjct: 61  RRQRWRHAPTVTESADTAAAYAHLERHDTGDDSAAQERVANSTKASKKDGSHLVAKALGM 120

Query: 121 DTEPKATGNKMGSFFDCNICLAVAKDPILTCCGHLFCWSCFYQLSYVHSNAKECPACQGE 180
           D E K TGNKMGSF+DCNICL VAKDPILTCCGHLFCWSCFYQLSYVHSNAKECP CQGE
Sbjct: 121 DPELKPTGNKMGSFYDCNICLDVAKDPILTCCGHLFCWSCFYQLSYVHSNAKECPECQGE 180

Query: 181 VTDTSIIPIYGHGNANRAQKSKRNDSGLKVPPRPRAQRIESMRQQILLRGASSSLIEERI 240
           VTDTSIIPIYGHGN NRAQKSK NDSGLKVPPRPRAQRIESMRQQIL +GASSSLIEERI
Sbjct: 181 VTDTSIIPIYGHGNGNRAQKSKPNDSGLKVPPRPRAQRIESMRQQILFQGASSSLIEERI 240

Query: 241 QQISNMIGAMGEQSRAQNFDVTHGRTERTNLLGRRRRSSYALPVVENESNQQSRSLQVSR 300
           QQISNMIGAMGEQSR+QN D+ HGR ERTNLLGRRRR S  LPV ENESNQQSRSLQVSR
Sbjct: 241 QQISNMIGAMGEQSRSQNSDIMHGRIERTNLLGRRRRPSQVLPVTENESNQQSRSLQVSR 300

Query: 301 LLLQGAASFSSLSSALNSAMDSAERLVEDLETYIHNHSASRSRPHSLPNNPNNGDALSGI 360
           LLLQGAASFSSLSSALNSAMDSAERLVEDLETYIHNH ASRSRPHSLPNNPNNGD LSGI
Sbjct: 301 LLLQGAASFSSLSSALNSAMDSAERLVEDLETYIHNHGASRSRPHSLPNNPNNGDPLSGI 360

Query: 361 PATVRSDGMTPDPAGGITFMVPQFESSSRSMDIASNIDHLGNQTSTATEFNRTILPSSST 420
            ATVRSDG+ PDP GGITF+VPQFESSSRSMDIA+NI  L NQTS+ TEF+R ILPS ST
Sbjct: 361 AATVRSDGIPPDPVGGITFLVPQFESSSRSMDIATNISRLENQTSSGTEFDRIILPSPST 420

Query: 421 RRRSELPLRSDMDNEILQEARRRRLG 447
           RRRSELP RSD+DNE LQE RRRRLG
Sbjct: 421 RRRSELPRRSDLDNENLQERRRRRLG 446

BLAST of MS016137 vs. ExPASy TrEMBL
Match: A0A1S3CFG1 (uncharacterized protein LOC103500286 OS=Cucumis melo OX=3656 GN=LOC103500286 PE=4 SV=1)

HSP 1 Score: 734.9 bits (1896), Expect = 1.9e-208
Identity = 384/447 (85.91%), Postives = 410/447 (91.72%), Query Frame = 0

Query: 1   MLTGSNSREDIMDLDLNQEPLEQSYDSVIGLDSILNDLETAHGRIEERIRQLEAVTTRAT 60
           ML G+NSREDIMDLDLNQEPL+QSYDSV+GLD+IL+DLETAHGRIEERIRQLEAVTTRAT
Sbjct: 1   MLMGNNSREDIMDLDLNQEPLDQSYDSVLGLDTILSDLETAHGRIEERIRQLEAVTTRAT 60

Query: 61  RRQRWRHAPNFIEPADSAAADTHVERHETGDDS-TAQERIVHSGKTSKKDGTKLIAKALG 120
           RRQRWRHAP   EPA++ AA   +ER ET DDS +AQERI+HS KTSKK+G  L+AKALG
Sbjct: 61  RRQRWRHAPTVTEPAETPAAYALLERQETVDDSASAQERILHSEKTSKKNGPHLVAKALG 120

Query: 121 MDTEPKATGNKMGSFFDCNICLAVAKDPILTCCGHLFCWSCFYQLSYVHSNAKECPACQG 180
           MD EPKATGNKMGSFFDCNICL VAKDPILTCCGHLFCWSCFYQLSYVHSNAKECP CQG
Sbjct: 121 MDLEPKATGNKMGSFFDCNICLDVAKDPILTCCGHLFCWSCFYQLSYVHSNAKECPECQG 180

Query: 181 EVTDTSIIPIYGHGNANRAQKSKRNDSGLKVPPRPRAQRIESMRQQILLRGASSSLIEER 240
           EVTDTSIIPIYGHGN NRAQKSK NDSGLKVPPRPRAQRIESMRQQIL RGASSSLIEER
Sbjct: 181 EVTDTSIIPIYGHGNGNRAQKSKPNDSGLKVPPRPRAQRIESMRQQILFRGASSSLIEER 240

Query: 241 IQQISNMIGAMGEQSRAQNFDVTHGRTERTNLLGRRRRSSYALPVVENESNQQSRSLQVS 300
           IQQISNMIGAMGEQSR+QNFDVT GR ERTNLLGRRRR++  LPV ENE+NQQSRSLQVS
Sbjct: 241 IQQISNMIGAMGEQSRSQNFDVTQGRIERTNLLGRRRRATQVLPVPENENNQQSRSLQVS 300

Query: 301 RLLLQGAASFSSLSSALNSAMDSAERLVEDLETYIHNHSASRSRPHSLPNNPNNGDALSG 360
           RLLLQGAASFSSLSSALNSAMDSAERLVEDLETYIHNHSASRSRPHS+PNNPNNGD+LSG
Sbjct: 301 RLLLQGAASFSSLSSALNSAMDSAERLVEDLETYIHNHSASRSRPHSVPNNPNNGDSLSG 360

Query: 361 IPATVRSDGMTPDPAGGITFMVPQFESSSRSMDIASNIDHLGNQTSTATEFNRTILPSSS 420
           I  TV+SDG+ PDP GGITF+VPQFESSSRSMDIA+NI+ L +QTS ATEF+R ILPS S
Sbjct: 361 IVPTVQSDGIAPDPVGGITFLVPQFESSSRSMDIATNIERLESQTSNATEFDRIILPSPS 420

Query: 421 TRRRSELPLRSDMDNEILQEARRRRLG 447
           TRRRSELP RSDMDN +LQE RRRRLG
Sbjct: 421 TRRRSELPRRSDMDNVLLQERRRRRLG 447

BLAST of MS016137 vs. ExPASy TrEMBL
Match: A0A5A7UA47 (Zf-C3HC4_2 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold148G00470 PE=4 SV=1)

HSP 1 Score: 734.9 bits (1896), Expect = 1.9e-208
Identity = 384/447 (85.91%), Postives = 410/447 (91.72%), Query Frame = 0

Query: 1   MLTGSNSREDIMDLDLNQEPLEQSYDSVIGLDSILNDLETAHGRIEERIRQLEAVTTRAT 60
           ML G+NSREDIMDLDLNQEPL+QSYDSV+GLD+IL+DLETAHGRIEERIRQLEAVTTRAT
Sbjct: 1   MLMGNNSREDIMDLDLNQEPLDQSYDSVLGLDTILSDLETAHGRIEERIRQLEAVTTRAT 60

Query: 61  RRQRWRHAPNFIEPADSAAADTHVERHETGDDS-TAQERIVHSGKTSKKDGTKLIAKALG 120
           RRQRWRHAP   EPA++ AA   +ER ET DDS +AQERI+HS KTSKK+G  L+AKALG
Sbjct: 61  RRQRWRHAPTVTEPAETPAAYALLERQETVDDSASAQERILHSEKTSKKNGPHLVAKALG 120

Query: 121 MDTEPKATGNKMGSFFDCNICLAVAKDPILTCCGHLFCWSCFYQLSYVHSNAKECPACQG 180
           MD EPKATGNKMGSFFDCNICL VAKDPILTCCGHLFCWSCFYQLSYVHSNAKECP CQG
Sbjct: 121 MDLEPKATGNKMGSFFDCNICLDVAKDPILTCCGHLFCWSCFYQLSYVHSNAKECPECQG 180

Query: 181 EVTDTSIIPIYGHGNANRAQKSKRNDSGLKVPPRPRAQRIESMRQQILLRGASSSLIEER 240
           EVTDTSIIPIYGHGN NRAQKSK NDSGLKVPPRPRAQRIESMRQQIL RGASSSLIEER
Sbjct: 181 EVTDTSIIPIYGHGNGNRAQKSKPNDSGLKVPPRPRAQRIESMRQQILFRGASSSLIEER 240

Query: 241 IQQISNMIGAMGEQSRAQNFDVTHGRTERTNLLGRRRRSSYALPVVENESNQQSRSLQVS 300
           IQQISNMIGAMGEQSR+QNFDVT GR ERTNLLGRRRR++  LPV ENE+NQQSRSLQVS
Sbjct: 241 IQQISNMIGAMGEQSRSQNFDVTQGRIERTNLLGRRRRATQVLPVPENENNQQSRSLQVS 300

Query: 301 RLLLQGAASFSSLSSALNSAMDSAERLVEDLETYIHNHSASRSRPHSLPNNPNNGDALSG 360
           RLLLQGAASFSSLSSALNSAMDSAERLVEDLETYIHNHSASRSRPHS+PNNPNNGD+LSG
Sbjct: 301 RLLLQGAASFSSLSSALNSAMDSAERLVEDLETYIHNHSASRSRPHSVPNNPNNGDSLSG 360

Query: 361 IPATVRSDGMTPDPAGGITFMVPQFESSSRSMDIASNIDHLGNQTSTATEFNRTILPSSS 420
           I  TV+SDG+ PDP GGITF+VPQFESSSRSMDIA+NI+ L +QTS ATEF+R ILPS S
Sbjct: 361 IVPTVQSDGIAPDPVGGITFLVPQFESSSRSMDIATNIERLESQTSNATEFDRIILPSPS 420

Query: 421 TRRRSELPLRSDMDNEILQEARRRRLG 447
           TRRRSELP RSDMDN +LQE RRRRLG
Sbjct: 421 TRRRSELPRRSDMDNVLLQERRRRRLG 447

BLAST of MS016137 vs. TAIR 10
Match: AT2G44410.1 (RING/U-box superfamily protein )

HSP 1 Score: 190.7 bits (483), Expect = 2.6e-48
Identity = 115/257 (44.75%), Postives = 149/257 (57.98%), Query Frame = 0

Query: 10  DIMDLDLNQEPLEQSYDS---VIGLDSILNDLETAHGRIEERIRQLEAVTTRATRRQ-RW 69
           + MDLDLNQEP   S      +  L  +L +LE+A  RI+ERIRQLE + +R   R+   
Sbjct: 8   ETMDLDLNQEPSSDSESPGGLMTALSPMLEELESAQERIQERIRQLEVIVSRIREREITT 67

Query: 70  RHAPNFIEP---ADSAAADTHVERHETGDDSTAQERIVHSGKTSKKDGTKLIAKALGMDT 129
              P  + P    DS A   H           ++ER+V +G+      T LIAKAL M+ 
Sbjct: 68  TTTPALVSPNEHRDSTAGVIH---------ERSRERLVENGENK----TYLIAKALNME- 127

Query: 130 EPKATGNKMGSFFDCNICLAVAKDPILTCCGHLFCWSCFYQLSYVHSNAKECPACQGEVT 189
               T +  G FFDCNICL  A+DPILTCCGHLFCW CFYQL  ++ N KECP C GEVT
Sbjct: 128 ---KTSSVPGGFFDCNICLEKAEDPILTCCGHLFCWGCFYQLPLIYLNIKECPVCDGEVT 187

Query: 190 DTSIIPIYGHGNANRAQKSKRNDSGLKVPPRPRAQRIESMRQQILLRGAS--SSLIEERI 249
           D  +IPIYG+G+     K K  D G+ +PPRP A+R+ES+RQ+I+ RG         E I
Sbjct: 188 DAEVIPIYGNGDDCDGTKPKLEDCGISLPPRPNAKRVESVRQKIINRGNPFFPGHENEAI 247

Query: 250 QQISNMIGAMGEQSRAQ 258
           + I   I ++G Q+ AQ
Sbjct: 248 EHIRRTIDSIGLQALAQ 247

BLAST of MS016137 vs. TAIR 10
Match: AT3G58030.1 (RING/U-box superfamily protein )

HSP 1 Score: 121.3 bits (303), Expect = 1.9e-27
Identity = 143/485 (29.48%), Postives = 207/485 (42.68%), Query Frame = 0

Query: 10  DIMDLDLNQEP-----LEQSYDSVIGLDSILNDLETAHGRIEERIRQLEAVTTRATRRQR 69
           D M+LDLN  P     L+ + +  + L    ND            R  EAVT   TR + 
Sbjct: 7   DTMNLDLNLGPGPESDLQPAPNETVNLADWTNDPPE---------RSSEAVTRIRTRHRT 66

Query: 70  WRHAPNFIEPADSAAADTHVERHETGDDSTAQERIVHSGKTSKKDGTKLIA-----KALG 129
                N   P  S      +E ++   +S      + +G+ S++    L        ALG
Sbjct: 67  RFRQLNLPIPVLSETHTMAIELNQLMGNS-VNRAAMQTGEGSERGNEDLKMCENGDGALG 126

Query: 130 -------MDTEPKATGNKMGSFFDCNICLAVAKDPILTCCGHLFCWSCFYQLSYVHSNAK 189
                   D E K++G+  G+FFDCNICL ++K+P+LTCCGHL+CW C YQ   + S+AK
Sbjct: 127 DGVLDKKADVE-KSSGSD-GNFFDCNICLDLSKEPVLTCCGHLYCWPCLYQWLQI-SDAK 186

Query: 190 ECPACQGEVTDTSIIPIYGHGNANRAQKSKRNDSGLKVPPRPRAQRIESMRQQILLRGAS 249
           ECP C+GEVT  ++ PIYG GN  R  +   +    KVP RP A+RIES+R  I  R   
Sbjct: 187 ECPVCKGEVTSKTVTPIYGRGNHKREIEESLD---TKVPMRPHARRIESLRNTI-QRSPF 246

Query: 250 SSLIEERIQQISNMIGAMGEQ-SRAQNFDVTHGRTERTNLLGRRRRSSYAL--------- 309
           +  +EE I++I N             N + +    +R N +  R  +S  +         
Sbjct: 247 TIPMEEMIRRIQNRFDRDSTPVPDFSNREASERVNDRANSILNRLMTSRGVRSEQNQASA 306

Query: 310 -------------------PVVENESNQQSRSLQVSRLLLQGAASFSSLSSALNSAMDSA 369
                              P +E ESN +   L + R L        +  S   SA+ SA
Sbjct: 307 AAAAIVAASEDIDLNPNIAPDLEGESNTRFHPLLIRRQLQSHRV---ARISTFTSALSSA 366

Query: 370 ERLVEDLETYIHNHSASRS---RPHSLPNNPNNGDALSGIPATVRSDGMTPDPAGGITFM 429
           ERLV   + Y   H   R+   + H  P   ++ D+ S I A + S+             
Sbjct: 367 ERLV---DAYFRTHPLGRNHQEQNHHAPVVVDDRDSFSSIAAVINSE------------- 426

Query: 430 VPQFESSSRSMDIASNIDHLGNQTSTATEFNRTILPSSSTRRRSELPLR-SDMDNEILQE 445
                                +Q  TA E +   L +SS+RRR+E   R SD+D+   + 
Sbjct: 427 ---------------------SQVDTAVEIDSMALSTSSSRRRNENGSRVSDVDSADSRP 434

BLAST of MS016137 vs. TAIR 10
Match: AT3G58030.2 (RING/U-box superfamily protein )

HSP 1 Score: 121.3 bits (303), Expect = 1.9e-27
Identity = 143/485 (29.48%), Postives = 207/485 (42.68%), Query Frame = 0

Query: 10  DIMDLDLNQEP-----LEQSYDSVIGLDSILNDLETAHGRIEERIRQLEAVTTRATRRQR 69
           D M+LDLN  P     L+ + +  + L    ND            R  EAVT   TR + 
Sbjct: 7   DTMNLDLNLGPGPESDLQPAPNETVNLADWTNDPPE---------RSSEAVTRIRTRHRT 66

Query: 70  WRHAPNFIEPADSAAADTHVERHETGDDSTAQERIVHSGKTSKKDGTKLIA-----KALG 129
                N   P  S      +E ++   +S      + +G+ S++    L        ALG
Sbjct: 67  RFRQLNLPIPVLSETHTMAIELNQLMGNS-VNRAAMQTGEGSERGNEDLKMCENGDGALG 126

Query: 130 -------MDTEPKATGNKMGSFFDCNICLAVAKDPILTCCGHLFCWSCFYQLSYVHSNAK 189
                   D E K++G+  G+FFDCNICL ++K+P+LTCCGHL+CW C YQ   + S+AK
Sbjct: 127 DGVLDKKADVE-KSSGSD-GNFFDCNICLDLSKEPVLTCCGHLYCWPCLYQWLQI-SDAK 186

Query: 190 ECPACQGEVTDTSIIPIYGHGNANRAQKSKRNDSGLKVPPRPRAQRIESMRQQILLRGAS 249
           ECP C+GEVT  ++ PIYG GN  R  +   +    KVP RP A+RIES+R  I  R   
Sbjct: 187 ECPVCKGEVTSKTVTPIYGRGNHKREIEESLD---TKVPMRPHARRIESLRNTI-QRSPF 246

Query: 250 SSLIEERIQQISNMIGAMGEQ-SRAQNFDVTHGRTERTNLLGRRRRSSYAL--------- 309
           +  +EE I++I N             N + +    +R N +  R  +S  +         
Sbjct: 247 TIPMEEMIRRIQNRFDRDSTPVPDFSNREASERVNDRANSILNRLMTSRGVRSEQNQASA 306

Query: 310 -------------------PVVENESNQQSRSLQVSRLLLQGAASFSSLSSALNSAMDSA 369
                              P +E ESN +   L + R L        +  S   SA+ SA
Sbjct: 307 AAAAIVAASEDIDLNPNIAPDLEGESNTRFHPLLIRRQLQSHRV---ARISTFTSALSSA 366

Query: 370 ERLVEDLETYIHNHSASRS---RPHSLPNNPNNGDALSGIPATVRSDGMTPDPAGGITFM 429
           ERLV   + Y   H   R+   + H  P   ++ D+ S I A + S+             
Sbjct: 367 ERLV---DAYFRTHPLGRNHQEQNHHAPVVVDDRDSFSSIAAVINSE------------- 426

Query: 430 VPQFESSSRSMDIASNIDHLGNQTSTATEFNRTILPSSSTRRRSELPLR-SDMDNEILQE 445
                                +Q  TA E +   L +SS+RRR+E   R SD+D+   + 
Sbjct: 427 ---------------------SQVDTAVEIDSMALSTSSSRRRNENGSRVSDVDSADSRP 434

BLAST of MS016137 vs. TAIR 10
Match: AT3G58030.3 (RING/U-box superfamily protein )

HSP 1 Score: 121.3 bits (303), Expect = 1.9e-27
Identity = 143/485 (29.48%), Postives = 207/485 (42.68%), Query Frame = 0

Query: 10  DIMDLDLNQEP-----LEQSYDSVIGLDSILNDLETAHGRIEERIRQLEAVTTRATRRQR 69
           D M+LDLN  P     L+ + +  + L    ND            R  EAVT   TR + 
Sbjct: 7   DTMNLDLNLGPGPESDLQPAPNETVNLADWTNDPPE---------RSSEAVTRIRTRHRT 66

Query: 70  WRHAPNFIEPADSAAADTHVERHETGDDSTAQERIVHSGKTSKKDGTKLIA-----KALG 129
                N   P  S      +E ++   +S      + +G+ S++    L        ALG
Sbjct: 67  RFRQLNLPIPVLSETHTMAIELNQLMGNS-VNRAAMQTGEGSERGNEDLKMCENGDGALG 126

Query: 130 -------MDTEPKATGNKMGSFFDCNICLAVAKDPILTCCGHLFCWSCFYQLSYVHSNAK 189
                   D E K++G+  G+FFDCNICL ++K+P+LTCCGHL+CW C YQ   + S+AK
Sbjct: 127 DGVLDKKADVE-KSSGSD-GNFFDCNICLDLSKEPVLTCCGHLYCWPCLYQWLQI-SDAK 186

Query: 190 ECPACQGEVTDTSIIPIYGHGNANRAQKSKRNDSGLKVPPRPRAQRIESMRQQILLRGAS 249
           ECP C+GEVT  ++ PIYG GN  R  +   +    KVP RP A+RIES+R  I  R   
Sbjct: 187 ECPVCKGEVTSKTVTPIYGRGNHKREIEESLD---TKVPMRPHARRIESLRNTI-QRSPF 246

Query: 250 SSLIEERIQQISNMIGAMGEQ-SRAQNFDVTHGRTERTNLLGRRRRSSYAL--------- 309
           +  +EE I++I N             N + +    +R N +  R  +S  +         
Sbjct: 247 TIPMEEMIRRIQNRFDRDSTPVPDFSNREASERVNDRANSILNRLMTSRGVRSEQNQASA 306

Query: 310 -------------------PVVENESNQQSRSLQVSRLLLQGAASFSSLSSALNSAMDSA 369
                              P +E ESN +   L + R L        +  S   SA+ SA
Sbjct: 307 AAAAIVAASEDIDLNPNIAPDLEGESNTRFHPLLIRRQLQSHRV---ARISTFTSALSSA 366

Query: 370 ERLVEDLETYIHNHSASRS---RPHSLPNNPNNGDALSGIPATVRSDGMTPDPAGGITFM 429
           ERLV   + Y   H   R+   + H  P   ++ D+ S I A + S+             
Sbjct: 367 ERLV---DAYFRTHPLGRNHQEQNHHAPVVVDDRDSFSSIAAVINSE------------- 426

Query: 430 VPQFESSSRSMDIASNIDHLGNQTSTATEFNRTILPSSSTRRRSELPLR-SDMDNEILQE 445
                                +Q  TA E +   L +SS+RRR+E   R SD+D+   + 
Sbjct: 427 ---------------------SQVDTAVEIDSMALSTSSSRRRNENGSRVSDVDSADSRP 434

BLAST of MS016137 vs. TAIR 10
Match: AT3G58030.4 (RING/U-box superfamily protein )

HSP 1 Score: 121.3 bits (303), Expect = 1.9e-27
Identity = 143/485 (29.48%), Postives = 207/485 (42.68%), Query Frame = 0

Query: 10  DIMDLDLNQEP-----LEQSYDSVIGLDSILNDLETAHGRIEERIRQLEAVTTRATRRQR 69
           D M+LDLN  P     L+ + +  + L    ND            R  EAVT   TR + 
Sbjct: 7   DTMNLDLNLGPGPESDLQPAPNETVNLADWTNDPPE---------RSSEAVTRIRTRHRT 66

Query: 70  WRHAPNFIEPADSAAADTHVERHETGDDSTAQERIVHSGKTSKKDGTKLIA-----KALG 129
                N   P  S      +E ++   +S      + +G+ S++    L        ALG
Sbjct: 67  RFRQLNLPIPVLSETHTMAIELNQLMGNS-VNRAAMQTGEGSERGNEDLKMCENGDGALG 126

Query: 130 -------MDTEPKATGNKMGSFFDCNICLAVAKDPILTCCGHLFCWSCFYQLSYVHSNAK 189
                   D E K++G+  G+FFDCNICL ++K+P+LTCCGHL+CW C YQ   + S+AK
Sbjct: 127 DGVLDKKADVE-KSSGSD-GNFFDCNICLDLSKEPVLTCCGHLYCWPCLYQWLQI-SDAK 186

Query: 190 ECPACQGEVTDTSIIPIYGHGNANRAQKSKRNDSGLKVPPRPRAQRIESMRQQILLRGAS 249
           ECP C+GEVT  ++ PIYG GN  R  +   +    KVP RP A+RIES+R  I  R   
Sbjct: 187 ECPVCKGEVTSKTVTPIYGRGNHKREIEESLD---TKVPMRPHARRIESLRNTI-QRSPF 246

Query: 250 SSLIEERIQQISNMIGAMGEQ-SRAQNFDVTHGRTERTNLLGRRRRSSYAL--------- 309
           +  +EE I++I N             N + +    +R N +  R  +S  +         
Sbjct: 247 TIPMEEMIRRIQNRFDRDSTPVPDFSNREASERVNDRANSILNRLMTSRGVRSEQNQASA 306

Query: 310 -------------------PVVENESNQQSRSLQVSRLLLQGAASFSSLSSALNSAMDSA 369
                              P +E ESN +   L + R L        +  S   SA+ SA
Sbjct: 307 AAAAIVAASEDIDLNPNIAPDLEGESNTRFHPLLIRRQLQSHRV---ARISTFTSALSSA 366

Query: 370 ERLVEDLETYIHNHSASRS---RPHSLPNNPNNGDALSGIPATVRSDGMTPDPAGGITFM 429
           ERLV   + Y   H   R+   + H  P   ++ D+ S I A + S+             
Sbjct: 367 ERLV---DAYFRTHPLGRNHQEQNHHAPVVVDDRDSFSSIAAVINSE------------- 426

Query: 430 VPQFESSSRSMDIASNIDHLGNQTSTATEFNRTILPSSSTRRRSELPLR-SDMDNEILQE 445
                                +Q  TA E +   L +SS+RRR+E   R SD+D+   + 
Sbjct: 427 ---------------------SQVDTAVEIDSMALSTSSSRRRNENGSRVSDVDSADSRP 434

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022152676.13.1e-24599.55uncharacterized protein LOC111020338 [Momordica charantia][more]
XP_023527862.18.6e-21186.68uncharacterized protein LOC111790954 [Cucurbita pepo subsp. pepo] >XP_023527863.... [more]
XP_022934926.11.1e-21086.68uncharacterized protein LOC111441954 [Cucurbita moschata] >XP_022934927.1 unchar... [more]
XP_022983257.11.2e-20985.87uncharacterized protein LOC111481891 [Cucurbita maxima] >XP_022983258.1 uncharac... [more]
XP_038905049.13.0e-20885.23uncharacterized protein LOC120091205 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q094631.5e-1642.42E3 ubiquitin ligase rnf-5 OS=Caenorhabditis elegans OX=6239 GN=rnf-5 PE=1 SV=1[more]
Q6PC782.6e-1633.61E3 ubiquitin-protein ligase RNF185 OS=Danio rerio OX=7955 GN=rnf185 PE=2 SV=1[more]
Q91YT23.3e-1638.89E3 ubiquitin-protein ligase RNF185 OS=Mus musculus OX=10090 GN=Rnf185 PE=2 SV=1[more]
Q568Y33.3e-1638.89E3 ubiquitin-protein ligase RNF185 OS=Rattus norvegicus OX=10116 GN=Rnf185 PE=2 ... [more]
Q96GF17.5e-1642.05E3 ubiquitin-protein ligase RNF185 OS=Homo sapiens OX=9606 GN=RNF185 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1DIG11.5e-24599.55uncharacterized protein LOC111020338 OS=Momordica charantia OX=3673 GN=LOC111020... [more]
A0A6J1F4615.4e-21186.68uncharacterized protein LOC111441954 OS=Cucurbita moschata OX=3662 GN=LOC1114419... [more]
A0A6J1J7956.0e-21085.87uncharacterized protein LOC111481891 OS=Cucurbita maxima OX=3661 GN=LOC111481891... [more]
A0A1S3CFG11.9e-20885.91uncharacterized protein LOC103500286 OS=Cucumis melo OX=3656 GN=LOC103500286 PE=... [more]
A0A5A7UA471.9e-20885.91Zf-C3HC4_2 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
Match NameE-valueIdentityDescription
AT2G44410.12.6e-4844.75RING/U-box superfamily protein [more]
AT3G58030.11.9e-2729.48RING/U-box superfamily protein [more]
AT3G58030.21.9e-2729.48RING/U-box superfamily protein [more]
AT3G58030.31.9e-2729.48RING/U-box superfamily protein [more]
AT3G58030.41.9e-2729.48RING/U-box superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 35..55
NoneNo IPR availablePFAMPF13920zf-C3HC4_3coord: 136..183
e-value: 5.7E-7
score: 29.3
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 337..366
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 339..359
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 86..106
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 194..216
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 415..446
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 424..446
NoneNo IPR availablePANTHERPTHR12313:SF63ZINC FINGER, C3HC4 TYPE (RING FINGER) PROTEINcoord: 16..349
NoneNo IPR availableCDDcd16745RING-HC_AtRMA_likecoord: 135..179
e-value: 4.07029E-21
score: 83.8928
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 131..187
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 137..177
e-value: 2.5E-6
score: 37.0
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 137..178
score: 12.312242
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 111..226
e-value: 2.8E-15
score: 58.4
IPR045103E3 ubiquitin-protein ligase RNF5/RNF185-likePANTHERPTHR12313E3 UBIQUITIN-PROTEIN LIGASE RNF5-RELATEDcoord: 16..349

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS016137.1MS016137.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006511 ubiquitin-dependent protein catabolic process
cellular_component GO:0005783 endoplasmic reticulum
molecular_function GO:0061630 ubiquitin protein ligase activity