MS016082 (gene) Bitter gourd (TR) v1

Overview
NameMS016082
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionRNA polymerase sigma factor sigD
Locationscaffold9_2: 250875 .. 254433 (+)
RNA-Seq ExpressionMS016082
SyntenyMS016082
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCACTGCCAGCAGCTTATGTTCATTGCCCACTCAATCCCCAACGCTCCCATCCATCTCTCTCTCCTCACTTCCATCGCTCAAAACCCATCTTCCCCCACAATCCCATTTCCTCTTTCCCCCTTTCTCCACCAAGTTAGGGTCAAATTTCGACTGGAATGAGACACTAGCCGTCCTGCCGCCGCAGGAGACGGCGGTTCTGGCGGTGGAGGCCGCGAGAGAGCATGTGCTGCCGGAACCCACTTGGGTCTCTGAAACTTGGAGCTGTGGAAAGCTTGAGAGAGTGGAGGAGAGCTGTGGGGATGATGGGTTTGTGGGTTGGAAGAAGAAGAGAAGGAAAAGGAGGAAAGAGGTGGGGGAGAAAGATGAGGAAGGTGGGGTTGCCTTGTCTAGTGGCCCCTTGAGGCCTGGATATTTGACCCCAAAAGAAGAAGCAGAGCTTTGTCTGTGTCTCAAGGTTGGTTTTAGTAAGATTTAATGTGGTTTTTTGTCCTTGTCTGTGCCACCATTTTAGTCTTTTGGGAGCTTACTTGAAGAGATGACATATATTTGTGTGTATATTTTTGAGACCGTGAGTATCTGGATTAGTTTATGCACACTTCGATTAATCTTAATAGATGCTGCAATAAAAAAATTAGTAGAATTTTAGAATTTTAGATGGGTGGCATGCACTTCGACTAATCTTAGGAGACAATTATCATAACCTGCAATGAAAGAACCAGTAGAATTTTAAAATTTAGATAGGTGGCAACGAATTTAAACTCATAGCTGTATCTAGACTAGCTTTATGTATACTTCAACTAATCTTAGAACCATTGGATGCCCAGTAATGAAAGAACTAGTAAAATCTTAAAATTTTAGGTAGGTGGCAACAAATAAACCCATAGCTTTTTTTGTTTGAAAGAAACAATTTCATTGATGTATGAAATTGACCCATAGCTTTAAACTCTACTTTTTTAACTTGTTAGTGGTCATTGTTTGCTTGTATATATTTCTCTTTAAACCCATAGCTTTAGACAGAATTATTTCATTATATTATGATTTTCACTTGGTTTTAAAACTAGGACTTAAAATATATGTTTTGTTCTTCAATTTTTTAGTGTTTAGAGCATATTGTATTTAATAGAATCTGATGACAAACTAAATGCATGTGGCTTAGAATAGAACAAAGTCTGTAGGATGTCCACATGAGATTTCTTGATAGTGTAATATAGAAATCTAGCAGCAAGGATCAAAGTGAAAATTTATATAAAGGTGAGAGGCCAACAACAAAATGTTTGAAAATACAAGGATGAGAATGAAAGAAGTATAAAAATTTAGGACTAAAGTAGAATTCAAACCTTTGTATTAATACACTTCAAATCATGTAAAATTGTTTGCCATTTTGATACATGATGGTAAGCTTTTGCACATTATTTACACTTCACAGGAGGCAGCACAGCTTGAAAAGGCAAAAACTAGAATCATGGAAACTCAAGAGCATGAACCTACAAATAAGCAATTGGCCATTGCCACGGGCACCAATTTAAGGTGTATAGATAGGATTCTTTGTAGAGCAAAAGAATCAAGGGATCGGCTCATTCAATGCTATAGCAAACTAGTCGTCTCGATTGCTGCTCCATATCAAGGCAAAGGATTAAGCCTTCAAGATCTCACTCAGGTAGGTTGTTGCACATATAACCTCTTTTGGATTGTTTTTATTCTTTATTTTTTTTGCAGTTTACTAATAGGATCCGATTACAAGAAAATGACAATGTAACTATATCACAATTATCAAAGAGAGATTACAATATCAATAGCCTTTCGAGAGGGCTATCTCTCTCCCAAGACTCTTACTAGACTATTCCTGAAAACTATCCTCCCTTCCTCTCCCTAATTCCTCTATTTATAACGGCCACTTCAACCCTAATTAATTATCATACTGTCTGCAACTAATATACTAATAATATTTCCAACACATTTTACCCTCACATTTACTTAAAATGTTTGTTTGTTTTGTAGGAAGGAAACATTGGGCTTCTTATAGGGGCGGAAAGGTTTGAACCAAATAGAGGACATAAGTTATCGACTTATGCTTATTGGTGGATAAGACAAGCTATAATTCGAGCCTTAGATAACAAGTCGAGACTTGTTAGATTGCCGGTAAGATACTGTATAAATTCCTCATTTTCACAATTAGATCATCATACAGAAGATATTAGAGAAGTTTCAAATGATAGTTGAATCTTGCTCCATTAGAAGGAGCTCGTCTGTAGGCTTGTAGCCAACAAAATCAAAACTAAGTCAAAGTTATGACAAGAAACGTATAAAGTAACCACCAAGAATAAGACATACCAAGGTAATAAGGAAGAACTTTTAGTTCGAAACCTGATTTTGTTCGTGTAAGTTTGCTTTCTATTTAGGATATTAATTGTTGTATCACCTTTTCACAAAAAATGGAAAAATAAATCTGTATAAAAGTTTCTTTTTTCGTTTCTATTTCCACAAAGTTTTGTTAGCTAAAGATTTTGAGTTGTGGAAGGTGAGCTGGTAATTAGTGAATGCTCGGTTAGCTCGCTGTGTGCAGGGTCACATGGGCCAGATGGTAGCAAGAATAGCAGAAGCAAATAATCATCTTGGCATTAAGTTATCACGACTGCCCACCCACAAGGAAATTGCCGAGTCGCTCAACGTTCGTGTTTCAACGGTAAAATTGGTGACAGAAAGGAGTAAGCGCCCGATTTCGCTCAATCAACCAGTAACTGATCGAGGTTGCTTAACCCTGCAGGTACTTCTTACCAAAGTCAAACTTAGTCTTTTACCAAAGGGGATACGCCCTTTTTCATGCTTCTTGCCACTATTCTACAATAATTTACAACATTTCACTCATGATCCTCTATAATTGAAGTAATGCCAAAGTTTTTCTTTTTTTGGGGCGCTCTCAATCGGAGGTTGACTCCTCAAAGGGGTCGCATATCAACTAGTTATTTTTTCAGTTAGATTAACTCTTAGGATTTGCTTATTCTGGGATGACGAAGAATAATGGAGTTTTGCTTAGAAGAAGTCCAATCATTCTCTCCTGTTCTAGTAAACAAATGCCAGTGTCTGAGTCATTTTATGCAACATCGACTAATTTCACACGACAGGTACTTGACTTTTACAATATTTTACATCATAAAGCTCGAAGTATAATCCGTCCTAGATACATAGCCACCAATACCATGGTTCAAACCACCTACGAGTTTTTGTTTCAGTGATAGTTATGACATATTGAAATTCTCTCTTTCTCAATGTTTTGCCAGGAGATCATGCCAGGGCCAGACAATTTGACACCAGAGAATATGGTAATGAGAAAACTGATGAAGCAAGAACTGAGGAGGCTTCTTAATACTCTTAGCAAAAGAGAAGCTCACATAATGAGATTGTACTTTGGCCTTAATGGAGAGATTCCACAGTCTTTTGAGGAGATTGGAAAGTCAATGAACCTCTCGAGGGAGACGGTTCGGCGAGTCAATATCGCTGCATTGTCAAAACTAAAGAAAACTGACATTCTAGACTATCTTAAAGATTATGTAGTG

mRNA sequence

ATGGCCACTGCCAGCAGCTTATGTTCATTGCCCACTCAATCCCCAACGCTCCCATCCATCTCTCTCTCCTCACTTCCATCGCTCAAAACCCATCTTCCCCCACAATCCCATTTCCTCTTTCCCCCTTTCTCCACCAAGTTAGGGTCAAATTTCGACTGGAATGAGACACTAGCCGTCCTGCCGCCGCAGGAGACGGCGGTTCTGGCGGTGGAGGCCGCGAGAGAGCATGTGCTGCCGGAACCCACTTGGGTCTCTGAAACTTGGAGCTGTGGAAAGCTTGAGAGAGTGGAGGAGAGCTGTGGGGATGATGGGTTTGTGGGTTGGAAGAAGAAGAGAAGGAAAAGGAGGAAAGAGGTGGGGGAGAAAGATGAGGAAGGTGGGGTTGCCTTGTCTAGTGGCCCCTTGAGGCCTGGATATTTGACCCCAAAAGAAGAAGCAGAGCTTTGTCTGTGTCTCAAGGAGGCAGCACAGCTTGAAAAGGCAAAAACTAGAATCATGGAAACTCAAGAGCATGAACCTACAAATAAGCAATTGGCCATTGCCACGGGCACCAATTTAAGGTGTATAGATAGGATTCTTTGTAGAGCAAAAGAATCAAGGGATCGGCTCATTCAATGCTATAGCAAACTAGTCGTCTCGATTGCTGCTCCATATCAAGGCAAAGGATTAAGCCTTCAAGATCTCACTCAGGAAGGAAACATTGGGCTTCTTATAGGGGCGGAAAGGTTTGAACCAAATAGAGGACATAAGTTATCGACTTATGCTTATTGGTGGATAAGACAAGCTATAATTCGAGCCTTAGATAACAAGTCGAGACTTGTTAGATTGCCGGGTCACATGGGCCAGATGGTAGCAAGAATAGCAGAAGCAAATAATCATCTTGGCATTAAGTTATCACGACTGCCCACCCACAAGGAAATTGCCGAGTCGCTCAACGTTCGTGTTTCAACGGTAAAATTGGTGACAGAAAGGAGTAAGCGCCCGATTTCGCTCAATCAACCAGTAACTGATCGAGGTTGCTTAACCCTGCAGGAGATCATGCCAGGGCCAGACAATTTGACACCAGAGAATATGGTAATGAGAAAACTGATGAAGCAAGAACTGAGGAGGCTTCTTAATACTCTTAGCAAAAGAGAAGCTCACATAATGAGATTGTACTTTGGCCTTAATGGAGAGATTCCACAGTCTTTTGAGGAGATTGGAAAGTCAATGAACCTCTCGAGGGAGACGGTTCGGCGAGTCAATATCGCTGCATTGTCAAAACTAAAGAAAACTGACATTCTAGACTATCTTAAAGATTATGTAGTG

Coding sequence (CDS)

ATGGCCACTGCCAGCAGCTTATGTTCATTGCCCACTCAATCCCCAACGCTCCCATCCATCTCTCTCTCCTCACTTCCATCGCTCAAAACCCATCTTCCCCCACAATCCCATTTCCTCTTTCCCCCTTTCTCCACCAAGTTAGGGTCAAATTTCGACTGGAATGAGACACTAGCCGTCCTGCCGCCGCAGGAGACGGCGGTTCTGGCGGTGGAGGCCGCGAGAGAGCATGTGCTGCCGGAACCCACTTGGGTCTCTGAAACTTGGAGCTGTGGAAAGCTTGAGAGAGTGGAGGAGAGCTGTGGGGATGATGGGTTTGTGGGTTGGAAGAAGAAGAGAAGGAAAAGGAGGAAAGAGGTGGGGGAGAAAGATGAGGAAGGTGGGGTTGCCTTGTCTAGTGGCCCCTTGAGGCCTGGATATTTGACCCCAAAAGAAGAAGCAGAGCTTTGTCTGTGTCTCAAGGAGGCAGCACAGCTTGAAAAGGCAAAAACTAGAATCATGGAAACTCAAGAGCATGAACCTACAAATAAGCAATTGGCCATTGCCACGGGCACCAATTTAAGGTGTATAGATAGGATTCTTTGTAGAGCAAAAGAATCAAGGGATCGGCTCATTCAATGCTATAGCAAACTAGTCGTCTCGATTGCTGCTCCATATCAAGGCAAAGGATTAAGCCTTCAAGATCTCACTCAGGAAGGAAACATTGGGCTTCTTATAGGGGCGGAAAGGTTTGAACCAAATAGAGGACATAAGTTATCGACTTATGCTTATTGGTGGATAAGACAAGCTATAATTCGAGCCTTAGATAACAAGTCGAGACTTGTTAGATTGCCGGGTCACATGGGCCAGATGGTAGCAAGAATAGCAGAAGCAAATAATCATCTTGGCATTAAGTTATCACGACTGCCCACCCACAAGGAAATTGCCGAGTCGCTCAACGTTCGTGTTTCAACGGTAAAATTGGTGACAGAAAGGAGTAAGCGCCCGATTTCGCTCAATCAACCAGTAACTGATCGAGGTTGCTTAACCCTGCAGGAGATCATGCCAGGGCCAGACAATTTGACACCAGAGAATATGGTAATGAGAAAACTGATGAAGCAAGAACTGAGGAGGCTTCTTAATACTCTTAGCAAAAGAGAAGCTCACATAATGAGATTGTACTTTGGCCTTAATGGAGAGATTCCACAGTCTTTTGAGGAGATTGGAAAGTCAATGAACCTCTCGAGGGAGACGGTTCGGCGAGTCAATATCGCTGCATTGTCAAAACTAAAGAAAACTGACATTCTAGACTATCTTAAAGATTATGTAGTG

Protein sequence

MATASSLCSLPTQSPTLPSISLSSLPSLKTHLPPQSHFLFPPFSTKLGSNFDWNETLAVLPPQETAVLAVEAAREHVLPEPTWVSETWSCGKLERVEESCGDDGFVGWKKKRRKRRKEVGEKDEEGGVALSSGPLRPGYLTPKEEAELCLCLKEAAQLEKAKTRIMETQEHEPTNKQLAIATGTNLRCIDRILCRAKESRDRLIQCYSKLVVSIAAPYQGKGLSLQDLTQEGNIGLLIGAERFEPNRGHKLSTYAYWWIRQAIIRALDNKSRLVRLPGHMGQMVARIAEANNHLGIKLSRLPTHKEIAESLNVRVSTVKLVTERSKRPISLNQPVTDRGCLTLQEIMPGPDNLTPENMVMRKLMKQELRRLLNTLSKREAHIMRLYFGLNGEIPQSFEEIGKSMNLSRETVRRVNIAALSKLKKTDILDYLKDYVV
Homology
BLAST of MS016082 vs. NCBI nr
Match: XP_022152696.1 (RNA polymerase sigma factor sigD, chloroplastic [Momordica charantia])

HSP 1 Score: 855.1 bits (2208), Expect = 2.6e-244
Identity = 434/436 (99.54%), Postives = 435/436 (99.77%), Query Frame = 0

Query: 1   MATASSLCSLPTQSPTLPSISLSSLPSLKTHLPPQSHFLFPPFSTKLGSNFDWNETLAVL 60
           MATASSLCSLPTQSPTLPSISLSSLPSLKTHLPPQSHFLFPPFSTKLGSNFDWNET+AVL
Sbjct: 1   MATASSLCSLPTQSPTLPSISLSSLPSLKTHLPPQSHFLFPPFSTKLGSNFDWNETIAVL 60

Query: 61  PPQETAVLAVEAAREHVLPEPTWVSETWSCGKLERVEESCGDDGFVGWKKKRRKRRKEVG 120
           PPQETAVLAVEAAREHVLPEPTWVSETWSCGKLERVEESCGDDGFVGWKKKRRKRRKEVG
Sbjct: 61  PPQETAVLAVEAAREHVLPEPTWVSETWSCGKLERVEESCGDDGFVGWKKKRRKRRKEVG 120

Query: 121 EKDEEGGVALSSGPLRPGYLTPKEEAELCLCLKEAAQLEKAKTRIMETQEHEPTNKQLAI 180
           EK EEGGVALSSGPLRPGYLTPKEEAELCLCLKEAAQLEKAKTRIMETQEHEPTNKQLAI
Sbjct: 121 EKAEEGGVALSSGPLRPGYLTPKEEAELCLCLKEAAQLEKAKTRIMETQEHEPTNKQLAI 180

Query: 181 ATGTNLRCIDRILCRAKESRDRLIQCYSKLVVSIAAPYQGKGLSLQDLTQEGNIGLLIGA 240
           ATGTNLRCIDRILCRAKESRDRLIQCYSKLVVSIAAPYQGKGLSLQDLTQEGNIGLLIGA
Sbjct: 181 ATGTNLRCIDRILCRAKESRDRLIQCYSKLVVSIAAPYQGKGLSLQDLTQEGNIGLLIGA 240

Query: 241 ERFEPNRGHKLSTYAYWWIRQAIIRALDNKSRLVRLPGHMGQMVARIAEANNHLGIKLSR 300
           ERFEPNRGHKLSTYAYWWIRQAIIRALDNKSRLVRLPGHMGQMVARIAEANNHLGIKLSR
Sbjct: 241 ERFEPNRGHKLSTYAYWWIRQAIIRALDNKSRLVRLPGHMGQMVARIAEANNHLGIKLSR 300

Query: 301 LPTHKEIAESLNVRVSTVKLVTERSKRPISLNQPVTDRGCLTLQEIMPGPDNLTPENMVM 360
           LPTHKEIAESLNVRVSTVKLVTERSKRPISLNQPVTDRGCLTLQEIMPGPDNLTPENMVM
Sbjct: 301 LPTHKEIAESLNVRVSTVKLVTERSKRPISLNQPVTDRGCLTLQEIMPGPDNLTPENMVM 360

Query: 361 RKLMKQELRRLLNTLSKREAHIMRLYFGLNGEIPQSFEEIGKSMNLSRETVRRVNIAALS 420
           RKLMKQELRRLLNTLSKREAHIMRLYFGLNGEIPQSFEEIGKSMNLSRETVRRVNIAALS
Sbjct: 361 RKLMKQELRRLLNTLSKREAHIMRLYFGLNGEIPQSFEEIGKSMNLSRETVRRVNIAALS 420

Query: 421 KLKKTDILDYLKDYVV 437
           KLKKTDILDYLKDYVV
Sbjct: 421 KLKKTDILDYLKDYVV 436

BLAST of MS016082 vs. NCBI nr
Match: XP_038906215.1 (RNA polymerase sigma factor sigD, chloroplastic [Benincasa hispida])

HSP 1 Score: 726.9 bits (1875), Expect = 1.1e-205
Identity = 370/436 (84.86%), Postives = 396/436 (90.83%), Query Frame = 0

Query: 1   MATASSLCSLPTQSPTLPSISLSSLPSLKTHLPPQSHFLFPPFSTKLGSNFDWNETLAVL 60
           MAT SSLCSLPTQSPTLPSISLSSLPSLKTHLP Q HF FPPFSTKLG+NF  N+TL + 
Sbjct: 1   MATTSSLCSLPTQSPTLPSISLSSLPSLKTHLPQQPHFPFPPFSTKLGTNFINNDTLTLP 60

Query: 61  PPQETAVLAVEAAREHVLPEPTWVSETWSCGKLERVEESCGDDGFVGWKKKRRKRRKEVG 120
           PP E AVL VEAAREHVL +    SE WSC  LER+E SCG DG V WKKKRRKRRKEVG
Sbjct: 61  PPPEAAVLTVEAAREHVLVDARGFSEDWSCKNLERMEGSCGGDGTVAWKKKRRKRRKEVG 120

Query: 121 EKDEEGGVALSSGPLRPGYLTPKEEAELCLCLKEAAQLEKAKTRIMETQEHEPTNKQLAI 180
            K EE   ALSSGPLRPGYL+PKEEAELCLCLKEAAQLE+AKTRIMETQEHEPTN+QLAI
Sbjct: 121 LKAEENWAALSSGPLRPGYLSPKEEAELCLCLKEAAQLEEAKTRIMETQEHEPTNRQLAI 180

Query: 181 ATGTNLRCIDRILCRAKESRDRLIQCYSKLVVSIAAPYQGKGLSLQDLTQEGNIGLLIGA 240
           A GT L  IDRILCRA+ESRDRLI+CYSKLV+SIAAPYQGKGLSLQDLTQEGNIGLL GA
Sbjct: 181 ALGTKLGSIDRILCRARESRDRLIRCYSKLVISIAAPYQGKGLSLQDLTQEGNIGLLKGA 240

Query: 241 ERFEPNRGHKLSTYAYWWIRQAIIRALDNKSRLVRLPGHMGQMVARIAEANNHLGIKLSR 300
           ERFEPNRGHKLSTYAYWWIRQAIIRALD KSRLVRLPGHMGQMVARIAEANNHLG+KLSR
Sbjct: 241 ERFEPNRGHKLSTYAYWWIRQAIIRALDKKSRLVRLPGHMGQMVARIAEANNHLGLKLSR 300

Query: 301 LPTHKEIAESLNVRVSTVKLVTERSKRPISLNQPVTDRGCLTLQEIMPGPDNLTPENMVM 360
            PTHKE+AESL VRVSTV+LVTERSKRPIS+NQPV DRGCLTLQEIMPGPDNLTPENMVM
Sbjct: 301 PPTHKEVAESLKVRVSTVRLVTERSKRPISINQPVNDRGCLTLQEIMPGPDNLTPENMVM 360

Query: 361 RKLMKQELRRLLNTLSKREAHIMRLYFGLNGEIPQSFEEIGKSMNLSRETVRRVNIAALS 420
           RKLMKQE++RLLNTLSKREAHI+ LYFGLNGEIP+SFEEIGKS+NLSRET+R++N+A LS
Sbjct: 361 RKLMKQEVKRLLNTLSKREAHILSLYFGLNGEIPRSFEEIGKSLNLSRETIRQINVATLS 420

Query: 421 KLKKTDILDYLKDYVV 437
           KLK TDILDYLK+Y+V
Sbjct: 421 KLKNTDILDYLKEYLV 436

BLAST of MS016082 vs. NCBI nr
Match: KAA0048391.1 (RNA polymerase sigma factor sigD [Cucumis melo var. makuwa] >TYJ99868.1 RNA polymerase sigma factor sigD [Cucumis melo var. makuwa])

HSP 1 Score: 703.7 bits (1815), Expect = 9.6e-199
Identity = 361/436 (82.80%), Postives = 388/436 (88.99%), Query Frame = 0

Query: 1   MATASSLCSLPTQSPTLPSISLSSLPSLKTHLPPQSHFLFPPFSTKLGSNFDWNETLAVL 60
           MAT SSLCSLP QSPTLPSISLSSLPSLKTHLP Q HF FPPFS+KLG NF   +TL + 
Sbjct: 1   MATTSSLCSLPAQSPTLPSISLSSLPSLKTHLPQQPHFPFPPFSSKLGPNFISYDTLTLP 60

Query: 61  PPQETAVLAVEAAREHVLPEPTWVSETWSCGKLERVEESCGDDGFVGWKKKRRKRRKEVG 120
           PP E AVLAVEAA EHVL E    SE WS   +ER+EESC  DG V WKKKRRKRRKEVG
Sbjct: 61  PPPEAAVLAVEAAGEHVLVEARGFSEDWSSKNVERMEESCVGDGTVAWKKKRRKRRKEVG 120

Query: 121 EKDEEGGVALSSGPLRPGYLTPKEEAELCLCLKEAAQLEKAKTRIMETQEHEPTNKQLAI 180
            K EE  +ALSSGPLRPGYL+PKEEAELCLCLKEA  LEK K+RIMETQEHEPTNKQLAI
Sbjct: 121 VKAEENWIALSSGPLRPGYLSPKEEAELCLCLKEAVLLEKVKSRIMETQEHEPTNKQLAI 180

Query: 181 ATGTNLRCIDRILCRAKESRDRLIQCYSKLVVSIAAPYQGKGLSLQDLTQEGNIGLLIGA 240
           A GT   CIDRILCRA+ESRDRLI+CYSKLV+SIAAPYQGKGLSLQDLTQEGNIGLL GA
Sbjct: 181 AMGTKPGCIDRILCRARESRDRLIRCYSKLVISIAAPYQGKGLSLQDLTQEGNIGLLKGA 240

Query: 241 ERFEPNRGHKLSTYAYWWIRQAIIRALDNKSRLVRLPGHMGQMVARIAEANNHLGIKLSR 300
           ERFEP+RGHKLSTYAYWWIRQAIIRALD KSRLVRLPGHMGQMVARIAE NNHLGIKLSR
Sbjct: 241 ERFEPSRGHKLSTYAYWWIRQAIIRALDKKSRLVRLPGHMGQMVARIAEENNHLGIKLSR 300

Query: 301 LPTHKEIAESLNVRVSTVKLVTERSKRPISLNQPVTDRGCLTLQEIMPGPDNLTPENMVM 360
            PT++E+AESL VRVSTV+LV ERSKRPIS+NQPV DR CLTLQEI+PGPDNLTPENMVM
Sbjct: 301 PPTNEEVAESLKVRVSTVRLVMERSKRPISINQPVNDRSCLTLQEIIPGPDNLTPENMVM 360

Query: 361 RKLMKQELRRLLNTLSKREAHIMRLYFGLNGEIPQSFEEIGKSMNLSRETVRRVNIAALS 420
           RKLMKQEL+RLLNTLSKREAHI+ LYFGLNGEIPQSFEEIGKS+NLSRET+RR+NIA LS
Sbjct: 361 RKLMKQELKRLLNTLSKREAHILSLYFGLNGEIPQSFEEIGKSLNLSRETIRRINIATLS 420

Query: 421 KLKKTDILDYLKDYVV 437
           KLKKT++LDY+KDY+V
Sbjct: 421 KLKKTNVLDYIKDYLV 436

BLAST of MS016082 vs. NCBI nr
Match: XP_004149725.1 (RNA polymerase sigma factor sigD, chloroplastic [Cucumis sativus] >KGN58573.1 hypothetical protein Csa_000720 [Cucumis sativus])

HSP 1 Score: 703.4 bits (1814), Expect = 1.3e-198
Identity = 361/436 (82.80%), Postives = 384/436 (88.07%), Query Frame = 0

Query: 1   MATASSLCSLPTQSPTLPSISLSSLPSLKTHLPPQSHFLFPPFSTKLGSNFDWNETLAVL 60
           MAT SSLCSLP QSPTLPSISLSSLPSLKTHLP Q HF FPPFSTKLG N   N+TL + 
Sbjct: 1   MATTSSLCSLPAQSPTLPSISLSSLPSLKTHLPQQPHFPFPPFSTKLGPNIISNDTLTLP 60

Query: 61  PPQETAVLAVEAAREHVLPEPTWVSETWSCGKLERVEESCGDDGFVGWKKKRRKRRKEVG 120
           PP E AVLAVEAA EHVL E    SE WS   +E++EESC  DG V WKKKRRKRRKEVG
Sbjct: 61  PPPEAAVLAVEAAGEHVLVEAKGFSEDWSSKNVEKMEESCVGDGTVAWKKKRRKRRKEVG 120

Query: 121 EKDEEGGVALSSGPLRPGYLTPKEEAELCLCLKEAAQLEKAKTRIMETQEHEPTNKQLAI 180
            K EE  +ALSSGPLRPGYL+PKEEAELCLCLKEA  LE  KTRIMETQEHEPTNKQLAI
Sbjct: 121 VKAEENWIALSSGPLRPGYLSPKEEAELCLCLKEAVLLENVKTRIMETQEHEPTNKQLAI 180

Query: 181 ATGTNLRCIDRILCRAKESRDRLIQCYSKLVVSIAAPYQGKGLSLQDLTQEGNIGLLIGA 240
           A GT   CIDRILCRA+ESRDRLI+CYSKLV+SIAAPYQGKGLSLQDLTQEGNIGLL GA
Sbjct: 181 AMGTKSGCIDRILCRARESRDRLIRCYSKLVISIAAPYQGKGLSLQDLTQEGNIGLLKGA 240

Query: 241 ERFEPNRGHKLSTYAYWWIRQAIIRALDNKSRLVRLPGHMGQMVARIAEANNHLGIKLSR 300
           ERFEPNRGHKLSTYAYWWIRQAIIRALD KSRLVRLPGHMGQMVARIAE NNHLGIKLSR
Sbjct: 241 ERFEPNRGHKLSTYAYWWIRQAIIRALDKKSRLVRLPGHMGQMVARIAEENNHLGIKLSR 300

Query: 301 LPTHKEIAESLNVRVSTVKLVTERSKRPISLNQPVTDRGCLTLQEIMPGPDNLTPENMVM 360
            PT+KE+AESL V +ST++LV ERSKRPIS+NQPV DR CLTLQEIMPGPDNLTPENMVM
Sbjct: 301 PPTNKEVAESLKVHLSTIRLVMERSKRPISINQPVNDRSCLTLQEIMPGPDNLTPENMVM 360

Query: 361 RKLMKQELRRLLNTLSKREAHIMRLYFGLNGEIPQSFEEIGKSMNLSRETVRRVNIAALS 420
           RKLMKQEL+RLLNTLSKREA I+ LYFGLNGEIPQSFEEIGKS+NLSRET+R+ NIA LS
Sbjct: 361 RKLMKQELKRLLNTLSKREARILSLYFGLNGEIPQSFEEIGKSLNLSRETIRQTNIATLS 420

Query: 421 KLKKTDILDYLKDYVV 437
           KLKKTD+LDYLKDY+V
Sbjct: 421 KLKKTDVLDYLKDYLV 436

BLAST of MS016082 vs. NCBI nr
Match: XP_023528248.1 (RNA polymerase sigma factor sigD, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 693.7 bits (1789), Expect = 9.9e-196
Identity = 354/437 (81.01%), Postives = 389/437 (89.02%), Query Frame = 0

Query: 1   MATASSLCSLPTQSPTLPSISLSSLPSLKTHLPPQSHFLFPPFSTKLGSNFDWNETLAVL 60
           MAT SSLCSLP QSPTLPSISLSSLPSLKTHLP Q HF   PFSTKL  NF  N+TL   
Sbjct: 1   MATTSSLCSLPAQSPTLPSISLSSLPSLKTHLPQQPHFTISPFSTKLAPNFINNDTLTHP 60

Query: 61  PPQE-TAVLAVEAAREHVLPEPTWVSETWSCGKLERVEESCGDDGFVGWKKKRRKRRKEV 120
           PP E  AVLAVEAAREHVL E  W+S+ W C  LE++E+SCG DG V WKK+RRKRRKEV
Sbjct: 61  PPSEAAAVLAVEAAREHVLVEAGWLSKDWRCKNLEKMEQSCGGDGNVAWKKRRRKRRKEV 120

Query: 121 GEKDEEGGVALSSGPLRPGYLTPKEEAELCLCLKEAAQLEKAKTRIMETQEHEPTNKQLA 180
           GE  EE  VALS+GPLRPG+L+ KEEAELCLCLKEAAQ+EK KTRIMETQ+HEPTNKQLA
Sbjct: 121 GETAEENWVALSNGPLRPGHLSSKEEAELCLCLKEAAQIEKIKTRIMETQDHEPTNKQLA 180

Query: 181 IATGTNLRCIDRILCRAKESRDRLIQCYSKLVVSIAAPYQGKGLSLQDLTQEGNIGLLIG 240
           IA GT L  IDR+L RA+ESRDRLI+CYSKLV+SIAAPYQGKG+SLQDLTQEGNIGLL G
Sbjct: 181 IAMGTKLGYIDRVLWRARESRDRLIRCYSKLVISIAAPYQGKGISLQDLTQEGNIGLLKG 240

Query: 241 AERFEPNRGHKLSTYAYWWIRQAIIRALDNKSRLVRLPGHMGQMVARIAEANNHLGIKLS 300
           AERFEPNRGHKLSTYAYWWIRQ II+ALD KSRLVRLPGHMGQMVARIAEANNHLGIKLS
Sbjct: 241 AERFEPNRGHKLSTYAYWWIRQTIIQALDKKSRLVRLPGHMGQMVARIAEANNHLGIKLS 300

Query: 301 RLPTHKEIAESLNVRVSTVKLVTERSKRPISLNQPVTDRGCLTLQEIMPGPDNLTPENMV 360
           R+PTHKE+A+ L V VSTV+LVTERSKRPIS+NQPV DRGCLTLQEIMPGPDNLTPENMV
Sbjct: 301 RVPTHKEVAKYLKVPVSTVRLVTERSKRPISINQPVNDRGCLTLQEIMPGPDNLTPENMV 360

Query: 361 MRKLMKQELRRLLNTLSKREAHIMRLYFGLNGEIPQSFEEIGKSMNLSRETVRRVNIAAL 420
           +RKLMKQEL++LLNTLSKREAHI+ LYFGLNG+IPQSFEEIGKS+NLSRET+R+ NIA L
Sbjct: 361 IRKLMKQELKKLLNTLSKREAHILTLYFGLNGQIPQSFEEIGKSLNLSRETIRQTNIATL 420

Query: 421 SKLKKTDILDYLKDYVV 437
           +KLKK++ILDYLKDY+V
Sbjct: 421 AKLKKSNILDYLKDYLV 437

BLAST of MS016082 vs. ExPASy Swiss-Prot
Match: Q9ZSL6 (RNA polymerase sigma factor sigD, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=SIGD PE=2 SV=1)

HSP 1 Score: 323.6 bits (828), Expect = 3.5e-87
Identity = 175/345 (50.72%), Postives = 238/345 (68.99%), Query Frame = 0

Query: 104 GFVGWKKKRRKRRKEVG------EKDEEGGVALSS--------GPLRPGYLTPKEEAELC 163
           G    K+++R+RR+ VG      E++E  GV   +        G  R G+L+  EE +LC
Sbjct: 73  GIQSEKRRKRRRRRRVGYERLEPEEEENAGVEAEAETISVPVVGASRSGFLSRLEEVQLC 132

Query: 164 LCLKEAAQLEKAKTRIMETQEHEPTNKQLAIATGTNLRCIDRILCRAKESRDRLIQCYSK 223
           L LKE A+LE   T +   +E+E  +  LA   G   R  + ILCR KE+R+++ +CY +
Sbjct: 133 LYLKEGAKLENLGTSV---EENEMVSVLLASGRGKKKRSANEILCRRKEAREKITRCYRR 192

Query: 224 LVVSIAAPYQGKGLSLQDLTQEGNIGLLIGAERFEPNRGHKLSTYAYWWIRQAIIRALDN 283
           LVVSIA  YQGKGL+LQDL QEG+IGLL GAERF+P+RG+KLSTY YWWI+QAI+RA+ +
Sbjct: 193 LVVSIATGYQGKGLNLQDLIQEGSIGLLRGAERFDPDRGYKLSTYVYWWIKQAILRAIAH 252

Query: 284 KSRLVRLPGHMGQMVARIAEANNHLGIKLSRLPTHKEIAESLNVRVSTVKLVTERSKRPI 343
           KSRLV+LPG M ++ A++AEA+N L  KL R P+ +EIAE LN+ VS V+L  ERS+ P+
Sbjct: 253 KSRLVKLPGSMWELTAKVAEASNVLTRKLRRQPSCEEIAEHLNLNVSAVRLAVERSRSPV 312

Query: 344 SLNQPVTDRGCLTLQEIMPGPDNLTPENMVMRKLMKQELRRLLNTLSKREAHIMRLYFGL 403
           SL++  +  G +TLQEI+ GPD   PE MV R+ MK E+ +LL +L+ RE+ ++ LYFGL
Sbjct: 313 SLDRVASQNGRMTLQEIVRGPDETRPEEMVKREHMKHEIEQLLGSLTARESRVLGLYFGL 372

Query: 404 NGEIPQSFEEIGKSMNLSRETVRRVNIAALSKLKKTDILDYLKDY 435
           NGE P SFEEIGKS+ LSRE VR++N  AL KL+    ++ LK Y
Sbjct: 373 NGETPMSFEEIGKSLKLSRERVRQINGIALKKLRNVHNVNDLKIY 414

BLAST of MS016082 vs. ExPASy Swiss-Prot
Match: P38023 (RNA polymerase sigma factor SigA1 OS=Synechococcus elongatus (strain PCC 7942 / FACHB-805) OX=1140 GN=sigA1 PE=3 SV=2)

HSP 1 Score: 199.1 bits (505), Expect = 1.0e-49
Identity = 116/340 (34.12%), Postives = 196/340 (57.65%), Query Frame = 0

Query: 97  EESCGDDGFVGWKKKRRKRRKEVGEKDEEGGVALSSGPL-RPGYLTPKEEAELCLCLKEA 156
           E+S G+D     K K + R+        E  + L    + R   L   EE EL   + + 
Sbjct: 65  EDSVGEDEDAAAKAKAKVRKTYT-----EDSIRLYLQEIGRIRLLRADEEIELARQIADL 124

Query: 157 AQLEKAKTRIMETQEHEPTNKQLAIATGTNLRCIDRILCRAKESRDRLIQCYSKLVVSIA 216
             LE+ +  ++E  +  P++ + A A  + L    R L R + ++D+++Q   +LVVSIA
Sbjct: 125 LALERIRDELLEQLDRLPSDAEWAAAVDSPLDEFRRRLFRGRRAKDKMVQSNLRLVVSIA 184

Query: 217 APYQGKGLSLQDLTQEGNIGLLIGAERFEPNRGHKLSTYAYWWIRQAIIRALDNKSRLVR 276
             Y  +GLS QDL QEG++GL+  AE+F+  +G+K STYA WWIRQAI RA+ ++SR +R
Sbjct: 185 KKYMNRGLSFQDLIQEGSLGLIRAAEKFDHEKGYKFSTYATWWIRQAITRAIADQSRTIR 244

Query: 277 LPGHMGQMVARIAEANNHLGIKLSRLPTHKEIAESLNVRVSTVKLVTERSKRPISLNQPV 336
           LP H+ + ++RI +    L  ++ R PT +EIA  + + +  ++ + + ++ PISL  P+
Sbjct: 245 LPVHLYETISRIKKTTKLLSQEMGRKPTEEEIATRMEMTIEKLRFIAKSAQLPISLETPI 304

Query: 337 TDRGCLTLQEIMPGPDNLTPENMVMRKLMKQELRRLLNTLSKREAHIMRLYFGLNGEIPQ 396
                  L + +   D  TPE+ V + L++++L  +L+TLS RE  ++RL +GL+    +
Sbjct: 305 GKEEDSRLGDFIEA-DGETPEDEVAKNLLREDLEGVLSTLSPRERDVLRLRYGLDDGRMK 364

Query: 397 SFEEIGKSMNLSRETVRRVNIAALSKLKKTDILDYLKDYV 436
           + EEIG+  N++RE +R++   AL KL+  +    LK+Y+
Sbjct: 365 TLEEIGQLFNVTRERIRQIEAKALRKLRHPNRNSILKEYI 398

BLAST of MS016082 vs. ExPASy Swiss-Prot
Match: P26683 (RNA polymerase sigma factor SigA OS=Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576) OX=103690 GN=sigA PE=3 SV=1)

HSP 1 Score: 199.1 bits (505), Expect = 1.0e-49
Identity = 114/335 (34.03%), Postives = 193/335 (57.61%), Query Frame = 0

Query: 102 DDGFVGWKKKRRKRRKEVGEKDEEGGVALSSGPL-RPGYLTPKEEAELCLCLKEAAQLEK 161
           DD   G   K R+R +   +   E  + L    + R   L   EE EL   + +  +LE+
Sbjct: 56  DDAKSGKAAKSRRRTQSKKKHYTEDSIRLYLQEIGRIRLLRADEEIELARKIADLLELER 115

Query: 162 AKTRIMETQEHEPTNKQLAIATGTNLRCIDRILCRAKESRDRLIQCYSKLVVSIAAPYQG 221
            + R+ E  E +P + + A A    L      L   + ++D+++Q   +LVVSIA  Y  
Sbjct: 116 VRERLSEKLERDPRDSEWAEAVQLPLPAFRYRLHIGRRAKDKMVQSNLRLVVSIAKKYMN 175

Query: 222 KGLSLQDLTQEGNIGLLIGAERFEPNRGHKLSTYAYWWIRQAIIRALDNKSRLVRLPGHM 281
           +GLS QDL QEG++GL+  AE+F+  +G+K STYA WWIRQAI RA+ ++SR +RLP H+
Sbjct: 176 RGLSFQDLIQEGSLGLIRAAEKFDHEKGYKFSTYATWWIRQAITRAIADQSRTIRLPVHL 235

Query: 282 GQMVARIAEANNHLGIKLSRLPTHKEIAESLNVRVSTVKLVTERSKRPISLNQPVTDRGC 341
            + ++RI +    L  ++ R PT +EIA  + + +  ++ + + ++ PISL  P+     
Sbjct: 236 YETISRIKKTTKLLSQEMGRKPTEEEIATRMEMTIEKLRFIAKSAQLPISLETPIGKEED 295

Query: 342 LTLQEIMPGPDNLTPENMVMRKLMKQELRRLLNTLSKREAHIMRLYFGLNGEIPQSFEEI 401
             L + +   D  TPE+ V + L++++L ++L++LS RE  ++RL +GL+    ++ EEI
Sbjct: 296 SRLGDFIES-DGETPEDQVSKNLLREDLEKVLDSLSPRERDVLRLRYGLDDGRMKTLEEI 355

Query: 402 GKSMNLSRETVRRVNIAALSKLKKTDILDYLKDYV 436
           G+  N++RE +R++   AL KL+  +    LK+Y+
Sbjct: 356 GQIFNVTRERIRQIEAKALRKLRHPNRNSVLKEYI 389

BLAST of MS016082 vs. ExPASy Swiss-Prot
Match: O22056 (RNA polymerase sigma factor sigB OS=Arabidopsis thaliana OX=3702 GN=SIGB PE=2 SV=2)

HSP 1 Score: 196.8 bits (499), Expect = 5.0e-49
Identity = 111/327 (33.94%), Postives = 196/327 (59.94%), Query Frame = 0

Query: 110 KKRRKRRKEVGEKDEEGGVALSSGPLRPGYLTPKEEAELCLCLKEAAQLEKAKTRIMETQ 169
           KK+R   +EV   D    + +++   +   LT +EE EL   +++  +LE+ +T + E  
Sbjct: 245 KKKRLVAQEVDHNDPLRYLRMTTSSSK--LLTVREEHELSAGIQDLLKLERLQTELTERS 304

Query: 170 EHEPTNKQLAIATGTNLRCIDRILCRAKESRDRLIQCYSKLVVSIAAPYQGKGLSLQDLT 229
             +PT  Q A A G + + + + +      +D++I+   +LV+SIA  YQG G++LQDL 
Sbjct: 305 GRQPTFAQWASAAGVDQKSLRQRIHHGTLCKDKMIKSNIRLVISIAKNYQGAGMNLQDLV 364

Query: 230 QEGNIGLLIGAERFEPNRGHKLSTYAYWWIRQAIIRALDNKSRLVRLPGHMGQMVARIAE 289
           QEG  GL+ GAE+F+  +G K STYA+WWI+QA+ ++L ++SR++RLP HM +   R+ E
Sbjct: 365 QEGCRGLVRGAEKFDATKGFKFSTYAHWWIKQAVRKSLSDQSRMIRLPFHMVEATYRVKE 424

Query: 290 ANNHLGIKLSRLPTHKEIAESLNVRVSTVKLVTERSKRPISLNQPVTDRGCLTLQEIMPG 349
           A   L  +  + P ++EIAE+  + +  +  V    K P SL+Q +     L   E++  
Sbjct: 425 ARKQLYSETGKHPKNEEIAEATGLSMKRLMAVLLSPKPPRSLDQKIGMNQNLKPSEVIAD 484

Query: 350 PDNLTPENMVMRKLMKQELRRLLNTLSKREAHIMRLYFGLNGEIPQSFEEIGKSMNLSRE 409
           P+ +T E++++++ M+Q+L ++L++L  RE  ++R  FG+     ++ +EIG+ M +SRE
Sbjct: 485 PEAVTSEDILIKEFMRQDLDKVLDSLGTREKQVIRWRFGMEDGRMKTLQEIGEMMGVSRE 544

Query: 410 TVRRVNIAALSKLKKTDILDYLKDYVV 437
            VR++  +A  KLK     ++L+ Y+V
Sbjct: 545 RVRQIESSAFRKLKNKKRNNHLQQYLV 569

BLAST of MS016082 vs. ExPASy Swiss-Prot
Match: Q9LD95 (RNA polymerase sigma factor sigF, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=SIGF PE=1 SV=1)

HSP 1 Score: 195.3 bits (495), Expect = 1.5e-48
Identity = 115/323 (35.60%), Postives = 186/323 (57.59%), Query Frame = 0

Query: 102 DDGFVGWKKKRRKRRKEVGEKDEEGGVALSSGPLRPGYLTPKEEAELCLCLKEAAQLEKA 161
           D+G+V  K   +K+ K+  + D+   + L  GP     LT KEEAEL   ++   +LEK 
Sbjct: 211 DEGYVPQKTSAKKKYKQGADNDDALQLFL-WGPETKQLLTAKEEAELISHIQHLLKLEKV 270

Query: 162 KTRIMETQEHEPTNKQLAIATGTNLRCIDRILCRAKESRDRLIQCYSKLVVSIAAPYQGK 221
           KT++      EPT  + A A G +   +   + R + SR++LI    +LVV IA  YQ +
Sbjct: 271 KTKLESQNGCEPTIGEWAEAMGISSPVLKSDIHRGRSSREKLITANLRLVVHIAKQYQNR 330

Query: 222 GLSLQDLTQEGNIGLLIGAERFEPNRGHKLSTYAYWWIRQAIIRALDNKSRLVRLPGHMG 281
           GL+ QDL QEG++GL+   E+F+P  G + +TYAYWWIRQ+I +++   SR +RLP ++ 
Sbjct: 331 GLNFQDLLQEGSMGLMKSVEKFKPQSGCRFATYAYWWIRQSIRKSIFQNSRTIRLPENVY 390

Query: 282 QMVARIAEANNHLGIKLSRLPTHKEIAESLNVRVSTVKLVTERSKRPISLNQPVTDRGCL 341
            ++ +++EA      + +  P+ +E+A  + V    +  +   ++ P+S+ QP+      
Sbjct: 391 MLLGKVSEARKTCVQEGNYRPSKEELAGHVGVSTEKLDKLLYNTRTPLSMQQPIWSDQDT 450

Query: 342 TLQEIMPGPDNLTPENMVMRKLMKQELRRLLNTLSKREAHIMRLYFGLNGEIPQSFEEIG 401
           T QEI P     TP   V ++LM+  +R LLN LS +E  I++L FG++G   +S  EIG
Sbjct: 451 TFQEITPDSGIETPTMSVGKQLMRNHVRNLLNVLSPKERRIIKLRFGIDGGKQRSLSEIG 510

Query: 402 KSMNLSRETVRRVNIAALSKLKK 425
           +   LS+E VR++   AL +LK+
Sbjct: 511 EIYGLSKERVRQLESRALYRLKQ 532

BLAST of MS016082 vs. ExPASy TrEMBL
Match: A0A6J1DII5 (RNA polymerase sigma factor sigD, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111020353 PE=3 SV=1)

HSP 1 Score: 855.1 bits (2208), Expect = 1.2e-244
Identity = 434/436 (99.54%), Postives = 435/436 (99.77%), Query Frame = 0

Query: 1   MATASSLCSLPTQSPTLPSISLSSLPSLKTHLPPQSHFLFPPFSTKLGSNFDWNETLAVL 60
           MATASSLCSLPTQSPTLPSISLSSLPSLKTHLPPQSHFLFPPFSTKLGSNFDWNET+AVL
Sbjct: 1   MATASSLCSLPTQSPTLPSISLSSLPSLKTHLPPQSHFLFPPFSTKLGSNFDWNETIAVL 60

Query: 61  PPQETAVLAVEAAREHVLPEPTWVSETWSCGKLERVEESCGDDGFVGWKKKRRKRRKEVG 120
           PPQETAVLAVEAAREHVLPEPTWVSETWSCGKLERVEESCGDDGFVGWKKKRRKRRKEVG
Sbjct: 61  PPQETAVLAVEAAREHVLPEPTWVSETWSCGKLERVEESCGDDGFVGWKKKRRKRRKEVG 120

Query: 121 EKDEEGGVALSSGPLRPGYLTPKEEAELCLCLKEAAQLEKAKTRIMETQEHEPTNKQLAI 180
           EK EEGGVALSSGPLRPGYLTPKEEAELCLCLKEAAQLEKAKTRIMETQEHEPTNKQLAI
Sbjct: 121 EKAEEGGVALSSGPLRPGYLTPKEEAELCLCLKEAAQLEKAKTRIMETQEHEPTNKQLAI 180

Query: 181 ATGTNLRCIDRILCRAKESRDRLIQCYSKLVVSIAAPYQGKGLSLQDLTQEGNIGLLIGA 240
           ATGTNLRCIDRILCRAKESRDRLIQCYSKLVVSIAAPYQGKGLSLQDLTQEGNIGLLIGA
Sbjct: 181 ATGTNLRCIDRILCRAKESRDRLIQCYSKLVVSIAAPYQGKGLSLQDLTQEGNIGLLIGA 240

Query: 241 ERFEPNRGHKLSTYAYWWIRQAIIRALDNKSRLVRLPGHMGQMVARIAEANNHLGIKLSR 300
           ERFEPNRGHKLSTYAYWWIRQAIIRALDNKSRLVRLPGHMGQMVARIAEANNHLGIKLSR
Sbjct: 241 ERFEPNRGHKLSTYAYWWIRQAIIRALDNKSRLVRLPGHMGQMVARIAEANNHLGIKLSR 300

Query: 301 LPTHKEIAESLNVRVSTVKLVTERSKRPISLNQPVTDRGCLTLQEIMPGPDNLTPENMVM 360
           LPTHKEIAESLNVRVSTVKLVTERSKRPISLNQPVTDRGCLTLQEIMPGPDNLTPENMVM
Sbjct: 301 LPTHKEIAESLNVRVSTVKLVTERSKRPISLNQPVTDRGCLTLQEIMPGPDNLTPENMVM 360

Query: 361 RKLMKQELRRLLNTLSKREAHIMRLYFGLNGEIPQSFEEIGKSMNLSRETVRRVNIAALS 420
           RKLMKQELRRLLNTLSKREAHIMRLYFGLNGEIPQSFEEIGKSMNLSRETVRRVNIAALS
Sbjct: 361 RKLMKQELRRLLNTLSKREAHIMRLYFGLNGEIPQSFEEIGKSMNLSRETVRRVNIAALS 420

Query: 421 KLKKTDILDYLKDYVV 437
           KLKKTDILDYLKDYVV
Sbjct: 421 KLKKTDILDYLKDYVV 436

BLAST of MS016082 vs. ExPASy TrEMBL
Match: A0A5D3BP27 (RNA polymerase sigma factor sigD OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold474G00100 PE=3 SV=1)

HSP 1 Score: 703.7 bits (1815), Expect = 4.7e-199
Identity = 361/436 (82.80%), Postives = 388/436 (88.99%), Query Frame = 0

Query: 1   MATASSLCSLPTQSPTLPSISLSSLPSLKTHLPPQSHFLFPPFSTKLGSNFDWNETLAVL 60
           MAT SSLCSLP QSPTLPSISLSSLPSLKTHLP Q HF FPPFS+KLG NF   +TL + 
Sbjct: 1   MATTSSLCSLPAQSPTLPSISLSSLPSLKTHLPQQPHFPFPPFSSKLGPNFISYDTLTLP 60

Query: 61  PPQETAVLAVEAAREHVLPEPTWVSETWSCGKLERVEESCGDDGFVGWKKKRRKRRKEVG 120
           PP E AVLAVEAA EHVL E    SE WS   +ER+EESC  DG V WKKKRRKRRKEVG
Sbjct: 61  PPPEAAVLAVEAAGEHVLVEARGFSEDWSSKNVERMEESCVGDGTVAWKKKRRKRRKEVG 120

Query: 121 EKDEEGGVALSSGPLRPGYLTPKEEAELCLCLKEAAQLEKAKTRIMETQEHEPTNKQLAI 180
            K EE  +ALSSGPLRPGYL+PKEEAELCLCLKEA  LEK K+RIMETQEHEPTNKQLAI
Sbjct: 121 VKAEENWIALSSGPLRPGYLSPKEEAELCLCLKEAVLLEKVKSRIMETQEHEPTNKQLAI 180

Query: 181 ATGTNLRCIDRILCRAKESRDRLIQCYSKLVVSIAAPYQGKGLSLQDLTQEGNIGLLIGA 240
           A GT   CIDRILCRA+ESRDRLI+CYSKLV+SIAAPYQGKGLSLQDLTQEGNIGLL GA
Sbjct: 181 AMGTKPGCIDRILCRARESRDRLIRCYSKLVISIAAPYQGKGLSLQDLTQEGNIGLLKGA 240

Query: 241 ERFEPNRGHKLSTYAYWWIRQAIIRALDNKSRLVRLPGHMGQMVARIAEANNHLGIKLSR 300
           ERFEP+RGHKLSTYAYWWIRQAIIRALD KSRLVRLPGHMGQMVARIAE NNHLGIKLSR
Sbjct: 241 ERFEPSRGHKLSTYAYWWIRQAIIRALDKKSRLVRLPGHMGQMVARIAEENNHLGIKLSR 300

Query: 301 LPTHKEIAESLNVRVSTVKLVTERSKRPISLNQPVTDRGCLTLQEIMPGPDNLTPENMVM 360
            PT++E+AESL VRVSTV+LV ERSKRPIS+NQPV DR CLTLQEI+PGPDNLTPENMVM
Sbjct: 301 PPTNEEVAESLKVRVSTVRLVMERSKRPISINQPVNDRSCLTLQEIIPGPDNLTPENMVM 360

Query: 361 RKLMKQELRRLLNTLSKREAHIMRLYFGLNGEIPQSFEEIGKSMNLSRETVRRVNIAALS 420
           RKLMKQEL+RLLNTLSKREAHI+ LYFGLNGEIPQSFEEIGKS+NLSRET+RR+NIA LS
Sbjct: 361 RKLMKQELKRLLNTLSKREAHILSLYFGLNGEIPQSFEEIGKSLNLSRETIRRINIATLS 420

Query: 421 KLKKTDILDYLKDYVV 437
           KLKKT++LDY+KDY+V
Sbjct: 421 KLKKTNVLDYIKDYLV 436

BLAST of MS016082 vs. ExPASy TrEMBL
Match: A0A0A0L9T0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G687740 PE=3 SV=1)

HSP 1 Score: 703.4 bits (1814), Expect = 6.1e-199
Identity = 361/436 (82.80%), Postives = 384/436 (88.07%), Query Frame = 0

Query: 1   MATASSLCSLPTQSPTLPSISLSSLPSLKTHLPPQSHFLFPPFSTKLGSNFDWNETLAVL 60
           MAT SSLCSLP QSPTLPSISLSSLPSLKTHLP Q HF FPPFSTKLG N   N+TL + 
Sbjct: 1   MATTSSLCSLPAQSPTLPSISLSSLPSLKTHLPQQPHFPFPPFSTKLGPNIISNDTLTLP 60

Query: 61  PPQETAVLAVEAAREHVLPEPTWVSETWSCGKLERVEESCGDDGFVGWKKKRRKRRKEVG 120
           PP E AVLAVEAA EHVL E    SE WS   +E++EESC  DG V WKKKRRKRRKEVG
Sbjct: 61  PPPEAAVLAVEAAGEHVLVEAKGFSEDWSSKNVEKMEESCVGDGTVAWKKKRRKRRKEVG 120

Query: 121 EKDEEGGVALSSGPLRPGYLTPKEEAELCLCLKEAAQLEKAKTRIMETQEHEPTNKQLAI 180
            K EE  +ALSSGPLRPGYL+PKEEAELCLCLKEA  LE  KTRIMETQEHEPTNKQLAI
Sbjct: 121 VKAEENWIALSSGPLRPGYLSPKEEAELCLCLKEAVLLENVKTRIMETQEHEPTNKQLAI 180

Query: 181 ATGTNLRCIDRILCRAKESRDRLIQCYSKLVVSIAAPYQGKGLSLQDLTQEGNIGLLIGA 240
           A GT   CIDRILCRA+ESRDRLI+CYSKLV+SIAAPYQGKGLSLQDLTQEGNIGLL GA
Sbjct: 181 AMGTKSGCIDRILCRARESRDRLIRCYSKLVISIAAPYQGKGLSLQDLTQEGNIGLLKGA 240

Query: 241 ERFEPNRGHKLSTYAYWWIRQAIIRALDNKSRLVRLPGHMGQMVARIAEANNHLGIKLSR 300
           ERFEPNRGHKLSTYAYWWIRQAIIRALD KSRLVRLPGHMGQMVARIAE NNHLGIKLSR
Sbjct: 241 ERFEPNRGHKLSTYAYWWIRQAIIRALDKKSRLVRLPGHMGQMVARIAEENNHLGIKLSR 300

Query: 301 LPTHKEIAESLNVRVSTVKLVTERSKRPISLNQPVTDRGCLTLQEIMPGPDNLTPENMVM 360
            PT+KE+AESL V +ST++LV ERSKRPIS+NQPV DR CLTLQEIMPGPDNLTPENMVM
Sbjct: 301 PPTNKEVAESLKVHLSTIRLVMERSKRPISINQPVNDRSCLTLQEIMPGPDNLTPENMVM 360

Query: 361 RKLMKQELRRLLNTLSKREAHIMRLYFGLNGEIPQSFEEIGKSMNLSRETVRRVNIAALS 420
           RKLMKQEL+RLLNTLSKREA I+ LYFGLNGEIPQSFEEIGKS+NLSRET+R+ NIA LS
Sbjct: 361 RKLMKQELKRLLNTLSKREARILSLYFGLNGEIPQSFEEIGKSLNLSRETIRQTNIATLS 420

Query: 421 KLKKTDILDYLKDYVV 437
           KLKKTD+LDYLKDY+V
Sbjct: 421 KLKKTDVLDYLKDYLV 436

BLAST of MS016082 vs. ExPASy TrEMBL
Match: A0A6J1J3P0 (RNA polymerase sigma factor sigD, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111481519 PE=3 SV=1)

HSP 1 Score: 693.3 bits (1788), Expect = 6.3e-196
Identity = 354/437 (81.01%), Postives = 389/437 (89.02%), Query Frame = 0

Query: 1   MATASSLCSLPTQSPTLPSISLSSLPSLKTHLPPQSHFLFPPFSTKLGSNFDWNETLAVL 60
           MAT SSLCSLP QSPTLPSISLSSLPSLKTHLP Q HF   PFSTKL  NF  N+TL   
Sbjct: 1   MATTSSLCSLPAQSPTLPSISLSSLPSLKTHLPQQPHFTISPFSTKLAPNFISNDTLTHP 60

Query: 61  PPQE-TAVLAVEAAREHVLPEPTWVSETWSCGKLERVEESCGDDGFVGWKKKRRKRRKEV 120
           PP E  AVLAVEAAREHVL E  W+S+ W C  LE++E+SCG DG V WKK+RRKRRKEV
Sbjct: 61  PPSEAAAVLAVEAAREHVLVEAGWLSKDWRCKNLEKMEQSCGGDGNVAWKKRRRKRRKEV 120

Query: 121 GEKDEEGGVALSSGPLRPGYLTPKEEAELCLCLKEAAQLEKAKTRIMETQEHEPTNKQLA 180
           GE  EE  VALSSGPLRPG+L+ KEEAELCLCLKEAAQ+EK KTRIMETQ+HEPTNKQLA
Sbjct: 121 GETAEENWVALSSGPLRPGHLSSKEEAELCLCLKEAAQIEKTKTRIMETQDHEPTNKQLA 180

Query: 181 IATGTNLRCIDRILCRAKESRDRLIQCYSKLVVSIAAPYQGKGLSLQDLTQEGNIGLLIG 240
           IA GT L  IDR+L RA+ESRDRLI+CYSKLV+SIAAPYQGKG+SLQDLTQEGNIGLL G
Sbjct: 181 IAMGTKLGYIDRVLWRARESRDRLIRCYSKLVISIAAPYQGKGISLQDLTQEGNIGLLKG 240

Query: 241 AERFEPNRGHKLSTYAYWWIRQAIIRALDNKSRLVRLPGHMGQMVARIAEANNHLGIKLS 300
           AERFEPNRGHKLSTYAYWWIRQ II+ALD KSRLVRLPGHMGQMVARIAEANNHLGIKLS
Sbjct: 241 AERFEPNRGHKLSTYAYWWIRQTIIQALDKKSRLVRLPGHMGQMVARIAEANNHLGIKLS 300

Query: 301 RLPTHKEIAESLNVRVSTVKLVTERSKRPISLNQPVTDRGCLTLQEIMPGPDNLTPENMV 360
           R+PTHKE+A+ L V VSTV+LVTERSKRPIS+N+PV DRGCLTLQEIMPGPDNLTPENMV
Sbjct: 301 RVPTHKEVAKYLKVPVSTVRLVTERSKRPISINRPVNDRGCLTLQEIMPGPDNLTPENMV 360

Query: 361 MRKLMKQELRRLLNTLSKREAHIMRLYFGLNGEIPQSFEEIGKSMNLSRETVRRVNIAAL 420
           +RKLMKQEL+RLL+TLSKREAHI+ LYFGLNG+IPQSFEEIGKS+NLSRET+R+ NIA L
Sbjct: 361 IRKLMKQELKRLLSTLSKREAHILTLYFGLNGQIPQSFEEIGKSLNLSRETIRQTNIATL 420

Query: 421 SKLKKTDILDYLKDYVV 437
           +KLKK++ILDYLKDY+V
Sbjct: 421 AKLKKSNILDYLKDYLV 437

BLAST of MS016082 vs. ExPASy TrEMBL
Match: A0A6J1FAN3 (RNA polymerase sigma factor sigD, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111442316 PE=3 SV=1)

HSP 1 Score: 691.4 bits (1783), Expect = 2.4e-195
Identity = 353/437 (80.78%), Postives = 389/437 (89.02%), Query Frame = 0

Query: 1   MATASSLCSLPTQSPTLPSISLSSLPSLKTHLPPQSHFLFPPFSTKLGSNFDWNETLAVL 60
           MAT SSLCSLP QSPTLPSISLSSLPSLKTHLP Q HF   PFSTKL  NF  N+TL   
Sbjct: 1   MATTSSLCSLPAQSPTLPSISLSSLPSLKTHLPQQPHFTISPFSTKLAPNFINNDTLTHP 60

Query: 61  PPQE-TAVLAVEAAREHVLPEPTWVSETWSCGKLERVEESCGDDGFVGWKKKRRKRRKEV 120
           PP E  AVLAVEAAREHVL E  W+S+ WSC  LE++E+SCG  G V WKK+RRKRRKEV
Sbjct: 61  PPSEAAAVLAVEAAREHVLVEAGWLSKDWSCKNLEKMEQSCGGAGNVAWKKRRRKRRKEV 120

Query: 121 GEKDEEGGVALSSGPLRPGYLTPKEEAELCLCLKEAAQLEKAKTRIMETQEHEPTNKQLA 180
           GE  EE  VALS+GPLRPG+L+ KEEAELCLCLKEAAQ+EK KTRIMETQEHEPTNKQLA
Sbjct: 121 GETAEENWVALSNGPLRPGHLSSKEEAELCLCLKEAAQIEKIKTRIMETQEHEPTNKQLA 180

Query: 181 IATGTNLRCIDRILCRAKESRDRLIQCYSKLVVSIAAPYQGKGLSLQDLTQEGNIGLLIG 240
           IA GT L  IDR+L RA+ESRDRLI+CYSKLV+SIAAPYQGKG+SLQDLTQEGNIGLL G
Sbjct: 181 IAMGTKLGYIDRVLWRARESRDRLIRCYSKLVISIAAPYQGKGISLQDLTQEGNIGLLKG 240

Query: 241 AERFEPNRGHKLSTYAYWWIRQAIIRALDNKSRLVRLPGHMGQMVARIAEANNHLGIKLS 300
           AERFEPNRGHKLSTYAYWWIRQ II+ALD KSRLVRLPGHMGQMVA+IAEANNHLGIKLS
Sbjct: 241 AERFEPNRGHKLSTYAYWWIRQTIIQALDKKSRLVRLPGHMGQMVAKIAEANNHLGIKLS 300

Query: 301 RLPTHKEIAESLNVRVSTVKLVTERSKRPISLNQPVTDRGCLTLQEIMPGPDNLTPENMV 360
           R+PTHKE+A+ L V VSTV+LVTER+KRPIS+NQPV DRGCLTLQEIMPGPDNLTPENMV
Sbjct: 301 RVPTHKEVAKYLKVPVSTVRLVTERNKRPISINQPVNDRGCLTLQEIMPGPDNLTPENMV 360

Query: 361 MRKLMKQELRRLLNTLSKREAHIMRLYFGLNGEIPQSFEEIGKSMNLSRETVRRVNIAAL 420
           +RKLMKQEL++LLNTLSKREAHI+ LYFGLNG+IPQSFEEIGKS+NLSRET+R+ NIA L
Sbjct: 361 IRKLMKQELKKLLNTLSKREAHILTLYFGLNGQIPQSFEEIGKSLNLSRETIRQTNIATL 420

Query: 421 SKLKKTDILDYLKDYVV 437
           +KLKK++ILDYLKDY+V
Sbjct: 421 AKLKKSNILDYLKDYLV 437

BLAST of MS016082 vs. TAIR 10
Match: AT5G13730.1 (sigma factor 4 )

HSP 1 Score: 323.6 bits (828), Expect = 2.5e-88
Identity = 175/345 (50.72%), Postives = 238/345 (68.99%), Query Frame = 0

Query: 104 GFVGWKKKRRKRRKEVG------EKDEEGGVALSS--------GPLRPGYLTPKEEAELC 163
           G    K+++R+RR+ VG      E++E  GV   +        G  R G+L+  EE +LC
Sbjct: 73  GIQSEKRRKRRRRRRVGYERLEPEEEENAGVEAEAETISVPVVGASRSGFLSRLEEVQLC 132

Query: 164 LCLKEAAQLEKAKTRIMETQEHEPTNKQLAIATGTNLRCIDRILCRAKESRDRLIQCYSK 223
           L LKE A+LE   T +   +E+E  +  LA   G   R  + ILCR KE+R+++ +CY +
Sbjct: 133 LYLKEGAKLENLGTSV---EENEMVSVLLASGRGKKKRSANEILCRRKEAREKITRCYRR 192

Query: 224 LVVSIAAPYQGKGLSLQDLTQEGNIGLLIGAERFEPNRGHKLSTYAYWWIRQAIIRALDN 283
           LVVSIA  YQGKGL+LQDL QEG+IGLL GAERF+P+RG+KLSTY YWWI+QAI+RA+ +
Sbjct: 193 LVVSIATGYQGKGLNLQDLIQEGSIGLLRGAERFDPDRGYKLSTYVYWWIKQAILRAIAH 252

Query: 284 KSRLVRLPGHMGQMVARIAEANNHLGIKLSRLPTHKEIAESLNVRVSTVKLVTERSKRPI 343
           KSRLV+LPG M ++ A++AEA+N L  KL R P+ +EIAE LN+ VS V+L  ERS+ P+
Sbjct: 253 KSRLVKLPGSMWELTAKVAEASNVLTRKLRRQPSCEEIAEHLNLNVSAVRLAVERSRSPV 312

Query: 344 SLNQPVTDRGCLTLQEIMPGPDNLTPENMVMRKLMKQELRRLLNTLSKREAHIMRLYFGL 403
           SL++  +  G +TLQEI+ GPD   PE MV R+ MK E+ +LL +L+ RE+ ++ LYFGL
Sbjct: 313 SLDRVASQNGRMTLQEIVRGPDETRPEEMVKREHMKHEIEQLLGSLTARESRVLGLYFGL 372

Query: 404 NGEIPQSFEEIGKSMNLSRETVRRVNIAALSKLKKTDILDYLKDY 435
           NGE P SFEEIGKS+ LSRE VR++N  AL KL+    ++ LK Y
Sbjct: 373 NGETPMSFEEIGKSLKLSRERVRQINGIALKKLRNVHNVNDLKIY 414

BLAST of MS016082 vs. TAIR 10
Match: AT1G08540.1 (RNApolymerase sigma subunit 2 )

HSP 1 Score: 196.8 bits (499), Expect = 3.6e-50
Identity = 111/327 (33.94%), Postives = 196/327 (59.94%), Query Frame = 0

Query: 110 KKRRKRRKEVGEKDEEGGVALSSGPLRPGYLTPKEEAELCLCLKEAAQLEKAKTRIMETQ 169
           KK+R   +EV   D    + +++   +   LT +EE EL   +++  +LE+ +T + E  
Sbjct: 245 KKKRLVAQEVDHNDPLRYLRMTTSSSK--LLTVREEHELSAGIQDLLKLERLQTELTERS 304

Query: 170 EHEPTNKQLAIATGTNLRCIDRILCRAKESRDRLIQCYSKLVVSIAAPYQGKGLSLQDLT 229
             +PT  Q A A G + + + + +      +D++I+   +LV+SIA  YQG G++LQDL 
Sbjct: 305 GRQPTFAQWASAAGVDQKSLRQRIHHGTLCKDKMIKSNIRLVISIAKNYQGAGMNLQDLV 364

Query: 230 QEGNIGLLIGAERFEPNRGHKLSTYAYWWIRQAIIRALDNKSRLVRLPGHMGQMVARIAE 289
           QEG  GL+ GAE+F+  +G K STYA+WWI+QA+ ++L ++SR++RLP HM +   R+ E
Sbjct: 365 QEGCRGLVRGAEKFDATKGFKFSTYAHWWIKQAVRKSLSDQSRMIRLPFHMVEATYRVKE 424

Query: 290 ANNHLGIKLSRLPTHKEIAESLNVRVSTVKLVTERSKRPISLNQPVTDRGCLTLQEIMPG 349
           A   L  +  + P ++EIAE+  + +  +  V    K P SL+Q +     L   E++  
Sbjct: 425 ARKQLYSETGKHPKNEEIAEATGLSMKRLMAVLLSPKPPRSLDQKIGMNQNLKPSEVIAD 484

Query: 350 PDNLTPENMVMRKLMKQELRRLLNTLSKREAHIMRLYFGLNGEIPQSFEEIGKSMNLSRE 409
           P+ +T E++++++ M+Q+L ++L++L  RE  ++R  FG+     ++ +EIG+ M +SRE
Sbjct: 485 PEAVTSEDILIKEFMRQDLDKVLDSLGTREKQVIRWRFGMEDGRMKTLQEIGEMMGVSRE 544

Query: 410 TVRRVNIAALSKLKKTDILDYLKDYVV 437
            VR++  +A  KLK     ++L+ Y+V
Sbjct: 545 RVRQIESSAFRKLKNKKRNNHLQQYLV 569

BLAST of MS016082 vs. TAIR 10
Match: AT2G36990.1 (RNApolymerase sigma-subunit F )

HSP 1 Score: 195.3 bits (495), Expect = 1.0e-49
Identity = 115/323 (35.60%), Postives = 186/323 (57.59%), Query Frame = 0

Query: 102 DDGFVGWKKKRRKRRKEVGEKDEEGGVALSSGPLRPGYLTPKEEAELCLCLKEAAQLEKA 161
           D+G+V  K   +K+ K+  + D+   + L  GP     LT KEEAEL   ++   +LEK 
Sbjct: 211 DEGYVPQKTSAKKKYKQGADNDDALQLFL-WGPETKQLLTAKEEAELISHIQHLLKLEKV 270

Query: 162 KTRIMETQEHEPTNKQLAIATGTNLRCIDRILCRAKESRDRLIQCYSKLVVSIAAPYQGK 221
           KT++      EPT  + A A G +   +   + R + SR++LI    +LVV IA  YQ +
Sbjct: 271 KTKLESQNGCEPTIGEWAEAMGISSPVLKSDIHRGRSSREKLITANLRLVVHIAKQYQNR 330

Query: 222 GLSLQDLTQEGNIGLLIGAERFEPNRGHKLSTYAYWWIRQAIIRALDNKSRLVRLPGHMG 281
           GL+ QDL QEG++GL+   E+F+P  G + +TYAYWWIRQ+I +++   SR +RLP ++ 
Sbjct: 331 GLNFQDLLQEGSMGLMKSVEKFKPQSGCRFATYAYWWIRQSIRKSIFQNSRTIRLPENVY 390

Query: 282 QMVARIAEANNHLGIKLSRLPTHKEIAESLNVRVSTVKLVTERSKRPISLNQPVTDRGCL 341
            ++ +++EA      + +  P+ +E+A  + V    +  +   ++ P+S+ QP+      
Sbjct: 391 MLLGKVSEARKTCVQEGNYRPSKEELAGHVGVSTEKLDKLLYNTRTPLSMQQPIWSDQDT 450

Query: 342 TLQEIMPGPDNLTPENMVMRKLMKQELRRLLNTLSKREAHIMRLYFGLNGEIPQSFEEIG 401
           T QEI P     TP   V ++LM+  +R LLN LS +E  I++L FG++G   +S  EIG
Sbjct: 451 TFQEITPDSGIETPTMSVGKQLMRNHVRNLLNVLSPKERRIIKLRFGIDGGKQRSLSEIG 510

Query: 402 KSMNLSRETVRRVNIAALSKLKK 425
           +   LS+E VR++   AL +LK+
Sbjct: 511 EIYGLSKERVRQLESRALYRLKQ 532

BLAST of MS016082 vs. TAIR 10
Match: AT1G64860.1 (sigma factor A )

HSP 1 Score: 147.5 bits (371), Expect = 2.5e-35
Identity = 103/302 (34.11%), Postives = 160/302 (52.98%), Query Frame = 0

Query: 133 GPLRPGYLTPKEEAELCLCLKEAAQLEKAKTRIMETQEHEPTNKQLAIATGTNLRCIDRI 192
           G +    L+  E   L   +K   +L+  K+R+ +    EP+++QLA++   +   +   
Sbjct: 193 GVISEDVLSHVEVVRLSKKIKSGLRLDDHKSRLKDRLGCEPSDEQLAVSLKISRAELQAW 252

Query: 193 LCRAKESRDRLIQCYSKLVVSIAAPYQGKGLSLQDLTQEGNIGLLIGAERFEPNRGHKLS 252
           L     +R++L     +LV+SIA  Y   G  + DL Q G IGLL G E+F+ ++G ++S
Sbjct: 253 LMECHLAREKLAMSNVRLVMSIAQRYDNLGAEMSDLVQGGLIGLLRGIEKFDSSKGFRIS 312

Query: 253 TYAYWWIRQAIIRALDNKSRLVRLPGHMGQMVARIAEANNHLGIKLSRLPTHKEIAESLN 312
           TY YWWIRQ + RAL + SR +RLP H+ + +  I  A   L  K    P+   IAESLN
Sbjct: 313 TYVYWWIRQGVSRALVDNSRTLRLPTHLHERLGLIRNAKLRLQEK-GITPSIDRIAESLN 372

Query: 313 VRVSTVKLVTERSKRPISLNQPVTDRGCLTLQEIMPGPDNLT----------PENMVMRK 372
           +    V+  TE   +  SL     DR        +PG  + +          P +     
Sbjct: 373 MSQKKVRNATEAVSKVFSL-----DRDAFPSLNGLPGETHHSYIADTRLENNPWHGYDDL 432

Query: 373 LMKQELRRLLN-TLSKREAHIMRLYFGLNGEIPQSFEEIGKSMNLSRETVRRVNIAALSK 424
            +K+E+ +L++ TL +RE  I+RLY+GL+ E   ++E+I K + LSRE VR+V + AL K
Sbjct: 433 ALKEEVSKLISATLGEREKEIIRLYYGLDKEC-LTWEDISKRIGLSRERVRQVGLVALEK 487

BLAST of MS016082 vs. TAIR 10
Match: AT3G53920.1 (RNApolymerase sigma-subunit C )

HSP 1 Score: 135.2 bits (339), Expect = 1.3e-31
Identity = 90/297 (30.30%), Postives = 154/297 (51.85%), Query Frame = 0

Query: 143 KEEAELCLCLKEAAQLEKAKTRIMETQEHEPTNKQLAIATGTNLRCIDRILCRAKESRDR 202
           + E E+   +K  A +E+ +T++ E      +    A A G N + + R L      RD 
Sbjct: 276 RNETEMSTGVKIVADMERIRTQLEEESGKVASLSCWAAAAGMNEKLLMRNLHYGWYCRDE 335

Query: 203 LIQCYSKLVVSIAAPYQGKGLSLQDLTQEGNIGLLIGAERFEPNRGHKLSTYAYWWIRQA 262
           L++    LV+ +A  Y+G G++ +DL Q G +G+L GAERF+  RG+K STY  +WIR++
Sbjct: 336 LVKSTRSLVLFLARNYRGLGIAHEDLIQAGYVGVLQGAERFDHTRGYKFSTYVQYWIRKS 395

Query: 263 IIRALDNKSRLVRLPGHMGQMVARIAEANNHL----GIKLSRLPTHKEIAESLNVRVSTV 322
           +   +   +R V +P  + + +  I +A   L    GIK +     +EIA+     V  +
Sbjct: 396 MSTMVSRHARGVHIPSSIIRTINHIQKARKTLKTSHGIKYA---ADEEIAKLTGHSVKKI 455

Query: 323 KLVTERSKRPISLNQPVTDRGCLTLQEIMPGPDNLTPENMVMRKLMKQELRRLLNTLSKR 382
           +   +  K   S+++ V D       E  P     +PE  VMR+  ++++  LL  L  R
Sbjct: 456 RAANQCLKVVGSIDKKVGDCFTTKFLEFTPDTTMESPEEAVMRQSARRDIHDLLEGLEPR 515

Query: 383 EAHIMRLYFGLNGEIPQSFEEIGKSMNLSRETVRRVNIAALSKLKKTDILDYLKDYV 436
           E  +M L +GL    P+S EEIGK + +S+E +R++   A++KL+     + L+ Y+
Sbjct: 516 EKQVMVLRYGLQDYRPKSLEEIGKLLKVSKEWIRKIERRAMAKLRDQPNAEDLRYYL 569

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022152696.12.6e-24499.54RNA polymerase sigma factor sigD, chloroplastic [Momordica charantia][more]
XP_038906215.11.1e-20584.86RNA polymerase sigma factor sigD, chloroplastic [Benincasa hispida][more]
KAA0048391.19.6e-19982.80RNA polymerase sigma factor sigD [Cucumis melo var. makuwa] >TYJ99868.1 RNA poly... [more]
XP_004149725.11.3e-19882.80RNA polymerase sigma factor sigD, chloroplastic [Cucumis sativus] >KGN58573.1 hy... [more]
XP_023528248.19.9e-19681.01RNA polymerase sigma factor sigD, chloroplastic [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q9ZSL63.5e-8750.72RNA polymerase sigma factor sigD, chloroplastic OS=Arabidopsis thaliana OX=3702 ... [more]
P380231.0e-4934.12RNA polymerase sigma factor SigA1 OS=Synechococcus elongatus (strain PCC 7942 / ... [more]
P266831.0e-4934.03RNA polymerase sigma factor SigA OS=Nostoc sp. (strain PCC 7120 / SAG 25.82 / UT... [more]
O220565.0e-4933.94RNA polymerase sigma factor sigB OS=Arabidopsis thaliana OX=3702 GN=SIGB PE=2 SV... [more]
Q9LD951.5e-4835.60RNA polymerase sigma factor sigF, chloroplastic OS=Arabidopsis thaliana OX=3702 ... [more]
Match NameE-valueIdentityDescription
A0A6J1DII51.2e-24499.54RNA polymerase sigma factor sigD, chloroplastic OS=Momordica charantia OX=3673 G... [more]
A0A5D3BP274.7e-19982.80RNA polymerase sigma factor sigD OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
A0A0A0L9T06.1e-19982.80Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G687740 PE=3 SV=1[more]
A0A6J1J3P06.3e-19681.01RNA polymerase sigma factor sigD, chloroplastic OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A6J1FAN32.4e-19580.78RNA polymerase sigma factor sigD, chloroplastic OS=Cucurbita moschata OX=3662 GN... [more]
Match NameE-valueIdentityDescription
AT5G13730.12.5e-8850.72sigma factor 4 [more]
AT1G08540.13.6e-5033.94RNApolymerase sigma subunit 2 [more]
AT2G36990.11.0e-4935.60RNApolymerase sigma-subunit F [more]
AT1G64860.12.5e-3534.11sigma factor A [more]
AT3G53920.11.3e-3130.30RNApolymerase sigma-subunit C [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000943RNA polymerase sigma-70PRINTSPR00046SIGMA70FCTcoord: 375..387
score: 37.12
coord: 251..259
score: 74.91
coord: 411..422
score: 38.33
coord: 227..240
score: 56.08
coord: 396..411
score: 38.51
IPR007630RNA polymerase sigma-70 region 4PFAMPF04545Sigma70_r4coord: 371..424
e-value: 1.3E-11
score: 43.9
IPR007627RNA polymerase sigma-70 region 2PFAMPF04542Sigma70_r2coord: 203..272
e-value: 9.0E-19
score: 67.0
IPR014284RNA polymerase sigma-70 like domainTIGRFAMTIGR02937TIGR02937coord: 199..424
e-value: 8.7E-30
score: 101.5
IPR036388Winged helix-like DNA-binding domain superfamilyGENE3D1.10.10.10coord: 276..351
e-value: 1.6E-9
score: 40.0
IPR036388Winged helix-like DNA-binding domain superfamilyGENE3D1.10.10.10coord: 354..431
e-value: 1.3E-19
score: 71.8
IPR007624RNA polymerase sigma-70 region 3PFAMPF04539Sigma70_r3coord: 286..358
e-value: 2.0E-11
score: 43.8
NoneNo IPR availableGENE3D1.10.601.10RNA Polymerase Primary Sigma Factorcoord: 140..275
e-value: 1.8E-35
score: 125.1
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 116..136
NoneNo IPR availablePANTHERPTHR30603:SF47RNA POLYMERASE SIGMA FACTOR SIGD, CHLOROPLASTICcoord: 5..436
NoneNo IPR availablePANTHERPTHR30603RNA POLYMERASE SIGMA FACTOR RPOcoord: 5..436
NoneNo IPR availableCDDcd06171Sigma70_r4coord: 365..423
e-value: 3.19917E-8
score: 47.8694
IPR013324RNA polymerase sigma factor, region 3/4-likeSUPERFAMILY88659Sigma3 and sigma4 domains of RNA polymerase sigma factorscoord: 276..347
IPR013324RNA polymerase sigma factor, region 3/4-likeSUPERFAMILY88659Sigma3 and sigma4 domains of RNA polymerase sigma factorscoord: 334..425
IPR013325RNA polymerase sigma factor, region 2SUPERFAMILY88946Sigma2 domain of RNA polymerase sigma factorscoord: 137..272

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS016082.1MS016082.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006352 DNA-templated transcription, initiation
biological_process GO:0006355 regulation of transcription, DNA-templated
molecular_function GO:0003700 DNA-binding transcription factor activity