MS016073 (gene) Bitter gourd (TR) v1

Overview


Name	MS016073
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	acyl-CoA--sterol O-acyltransferase 1-like
Location	scaffold9_2: 181658 .. 182740 (+)
RNA-Seq Expression	MS016073
Synteny	MS016073

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAAATGAGATTATCAACTTCATTAATGTATGGCTCACAGTCTGTGCATCCCTCTGCTACTGCTACCTCATCGGAAAAAATGTCCGCCCCGGTGGCCGCCGCCTGATTTCCATCTTCCCCGTCGTCTGCCTCTTCCTCTACCTCCCGCTCCGCCTCTCCTCCGCCCATCTCGGCGGCCCCACCGCCTTCTTCATCGCCTGGCTCGCCAATTTCAAGCTCCTCCTCTTCTCTTTTGCCGGAGGCCCTCTATCTTCCGATCCCCCTCTCCCTCTCCGGCGATTTCTCGCCGTTTCCTGTTTGCCGATCAAGATTCAACGAAACCATCTTCAAATTTACCAAAATGGAGAAAACCCATTTCCGAAATCGAGCCCTAATGCCCCCCAAATCGAAACCCCATTACCCTCAAAATCGTTCCTCAGCTACGCGATTAAAGGTTTTCTAGTGGCGTTAATGGTGAAACTCTACGATTACAGCGAATTTATGCATCCGAAACTTGTTATGTGCATCTATAGTTGGCACATTTACTTTCTCCTCGAAATAATCCTCGCCGCCGCGAAATTCCTCGCCCGGAATCTCCTCGGAGCCGAGCTCGAGCCGCAGTTCAACGATCCGTACTTCTCCACTTCGCTCCAGGATTTTTGGGGACGGAGATGGAATCTGATGGTGACGGGCATTTTGAATCCGACGGTGTACAAGCCAACGATCCGGCTGGCGGCCCCCGTCGTCGGCCGGAAGTGGGCGCCGCTGCTGGCAGTGCTCGCGACGTTTTTCGTGTCGGCGATGATGCACGAGCTGATCTTTTACTACTTGGGCCGGATGAGGCCCGATTGGGAGGTGTCGCGATTCTTCGCCGTCCATGGGGTGGCTCTGACGTTGGAGATTTTGGCGAAGAAGGCGTGGCCGGAGAAGTGCCGGCGGGTGCCGGCGGTGGTTTCGGTGCCGTTGACGGTGGGGTTCGTGTTCGTCACCGGAATTTGGATGTTCTTCCCGCAGTTCGTCCGGTGTGGGATCGACGTCAAGGCGTTCGCCGAGTACGCGGCGGTGAGCGATTTTTTCACAAAGCGCCTCTTTCTCACCGGC

mRNA sequence

ATGGAAAATGAGATTATCAACTTCATTAATGTATGGCTCACAGTCTGTGCATCCCTCTGCTACTGCTACCTCATCGGAAAAAATGTCCGCCCCGGTGGCCGCCGCCTGATTTCCATCTTCCCCGTCGTCTGCCTCTTCCTCTACCTCCCGCTCCGCCTCTCCTCCGCCCATCTCGGCGGCCCCACCGCCTTCTTCATCGCCTGGCTCGCCAATTTCAAGCTCCTCCTCTTCTCTTTTGCCGGAGGCCCTCTATCTTCCGATCCCCCTCTCCCTCTCCGGCGATTTCTCGCCGTTTCCTGTTTGCCGATCAAGATTCAACGAAACCATCTTCAAATTTACCAAAATGGAGAAAACCCATTTCCGAAATCGAGCCCTAATGCCCCCCAAATCGAAACCCCATTACCCTCAAAATCGTTCCTCAGCTACGCGATTAAAGGTTTTCTAGTGGCGTTAATGGTGAAACTCTACGATTACAGCGAATTTATGCATCCGAAACTTGTTATGTGCATCTATAGTTGGCACATTTACTTTCTCCTCGAAATAATCCTCGCCGCCGCGAAATTCCTCGCCCGGAATCTCCTCGGAGCCGAGCTCGAGCCGCAGTTCAACGATCCGTACTTCTCCACTTCGCTCCAGGATTTTTGGGGACGGAGATGGAATCTGATGGTGACGGGCATTTTGAATCCGACGGTGTACAAGCCAACGATCCGGCTGGCGGCCCCCGTCGTCGGCCGGAAGTGGGCGCCGCTGCTGGCAGTGCTCGCGACGTTTTTCGTGTCGGCGATGATGCACGAGCTGATCTTTTACTACTTGGGCCGGATGAGGCCCGATTGGGAGGTGTCGCGATTCTTCGCCGTCCATGGGGTGGCTCTGACGTTGGAGATTTTGGCGAAGAAGGCGTGGCCGGAGAAGTGCCGGCGGGTGCCGGCGGTGGTTTCGGTGCCGTTGACGGTGGGGTTCGTGTTCGTCACCGGAATTTGGATGTTCTTCCCGCAGTTCGTCCGGTGTGGGATCGACGTCAAGGCGTTCGCCGAGTACGCGGCGGTGAGCGATTTTTTCACAAAGCGCCTCTTTCTCACCGGC

Coding sequence (CDS)

ATGGAAAATGAGATTATCAACTTCATTAATGTATGGCTCACAGTCTGTGCATCCCTCTGCTACTGCTACCTCATCGGAAAAAATGTCCGCCCCGGTGGCCGCCGCCTGATTTCCATCTTCCCCGTCGTCTGCCTCTTCCTCTACCTCCCGCTCCGCCTCTCCTCCGCCCATCTCGGCGGCCCCACCGCCTTCTTCATCGCCTGGCTCGCCAATTTCAAGCTCCTCCTCTTCTCTTTTGCCGGAGGCCCTCTATCTTCCGATCCCCCTCTCCCTCTCCGGCGATTTCTCGCCGTTTCCTGTTTGCCGATCAAGATTCAACGAAACCATCTTCAAATTTACCAAAATGGAGAAAACCCATTTCCGAAATCGAGCCCTAATGCCCCCCAAATCGAAACCCCATTACCCTCAAAATCGTTCCTCAGCTACGCGATTAAAGGTTTTCTAGTGGCGTTAATGGTGAAACTCTACGATTACAGCGAATTTATGCATCCGAAACTTGTTATGTGCATCTATAGTTGGCACATTTACTTTCTCCTCGAAATAATCCTCGCCGCCGCGAAATTCCTCGCCCGGAATCTCCTCGGAGCCGAGCTCGAGCCGCAGTTCAACGATCCGTACTTCTCCACTTCGCTCCAGGATTTTTGGGGACGGAGATGGAATCTGATGGTGACGGGCATTTTGAATCCGACGGTGTACAAGCCAACGATCCGGCTGGCGGCCCCCGTCGTCGGCCGGAAGTGGGCGCCGCTGCTGGCAGTGCTCGCGACGTTTTTCGTGTCGGCGATGATGCACGAGCTGATCTTTTACTACTTGGGCCGGATGAGGCCCGATTGGGAGGTGTCGCGATTCTTCGCCGTCCATGGGGTGGCTCTGACGTTGGAGATTTTGGCGAAGAAGGCGTGGCCGGAGAAGTGCCGGCGGGTGCCGGCGGTGGTTTCGGTGCCGTTGACGGTGGGGTTCGTGTTCGTCACCGGAATTTGGATGTTCTTCCCGCAGTTCGTCCGGTGTGGGATCGACGTCAAGGCGTTCGCCGAGTACGCGGCGGTGAGCGATTTTTTCACAAAGCGCCTCTTTCTCACCGGC

Protein sequence

MENEIINFINVWLTVCASLCYCYLIGKNVRPGGRRLISIFPVVCLFLYLPLRLSSAHLGGPTAFFIAWLANFKLLLFSFAGGPLSSDPPLPLRRFLAVSCLPIKIQRNHLQIYQNGENPFPKSSPNAPQIETPLPSKSFLSYAIKGFLVALMVKLYDYSEFMHPKLVMCIYSWHIYFLLEIILAAAKFLARNLLGAELEPQFNDPYFSTSLQDFWGRRWNLMVTGILNPTVYKPTIRLAAPVVGRKWAPLLAVLATFFVSAMMHELIFYYLGRMRPDWEVSRFFAVHGVALTLEILAKKAWPEKCRRVPAVVSVPLTVGFVFVTGIWMFFPQFVRCGIDVKAFAEYAAVSDFFTKRLFLTG

Homology

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BLAST of MS016073 vs. NCBI nr
Match: XP_022152669.1 (acyl-CoA--sterol O-acyltransferase 1-like [Momordica charantia])

HSP 1 Score: 724.5 bits (1869), Expect = 4.4e-205
Identity = 356/361 (98.61%), Postives = 357/361 (98.89%), Query Frame = 0


Query: 1   MENEIINFINVWLTVCASLCYCYLIGKNVRPGGRRLISIFPVVCLFLYLPLRLSSAHLGG 60
           MENEIINFINVWLTVCASLCYCYLIGKNVRPGGRRLISIFPVVCLFLYLPLRLSSAHLGG
Sbjct: 1   MENEIINFINVWLTVCASLCYCYLIGKNVRPGGRRLISIFPVVCLFLYLPLRLSSAHLGG 60

Query: 61  PTAFFIAWLANFKLLLFSFAGGPLSSDPPLPLRRFLAVSCLPIKIQRNHLQIYQNGENPF 120
           PTAFFIAWLANFKLLLFSFAGGPLSSDPPLPLRRFLAVSCLPIKIQRNHLQI QNGEN  
Sbjct: 61  PTAFFIAWLANFKLLLFSFAGGPLSSDPPLPLRRFLAVSCLPIKIQRNHLQISQNGENTS 120

Query: 121 PKSSPNAPQIETPLPSKSFLSYAIKGFLVALMVKLYDYSEFMHPKLVMCIYSWHIYFLLE 180
           PKSSPNAPQIETPLPSKSFLSYAIKGFLVALMVKLYDYSEFMHPKLVMCIYSWHIYFLLE
Sbjct: 121 PKSSPNAPQIETPLPSKSFLSYAIKGFLVALMVKLYDYSEFMHPKLVMCIYSWHIYFLLE 180

Query: 181 IILAAAKFLARNLLGAELEPQFNDPYFSTSLQDFWGRRWNLMVTGILNPTVYKPTIRLAA 240
           IILAAAKFLARNLLGAELEPQFNDPYFSTSLQDFWGRRWNLMVTGILNPTVYKPTIRLAA
Sbjct: 181 IILAAAKFLARNLLGAELEPQFNDPYFSTSLQDFWGRRWNLMVTGILNPTVYKPTIRLAA 240

Query: 241 PVVGRKWAPLLAVLATFFVSAMMHELIFYYLGRMRPDWEVSRFFAVHGVALTLEILAKKA 300
           PVVGRKWAPLLAVLATFFVSAMMHELIFYYLGRMRPDWEVSRFFAVHGVALT+EILAKKA
Sbjct: 241 PVVGRKWAPLLAVLATFFVSAMMHELIFYYLGRMRPDWEVSRFFAVHGVALTVEILAKKA 300

Query: 301 WPEKCRRVPAVVSVPLTVGFVFVTGIWMFFPQFVRCGIDVKAFAEYAAVSDFFTKRLFLT 360
           WPEKCRRVPAVVSVPLTVGFVFVTGIWMFFPQFVRCGIDVKAFAEYAAVSDFFTKRLFL 
Sbjct: 301 WPEKCRRVPAVVSVPLTVGFVFVTGIWMFFPQFVRCGIDVKAFAEYAAVSDFFTKRLFLI 360

Query: 361 G 362
           G
Sbjct: 361 G 361

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BLAST of MS016073 vs. NCBI nr
Match: XP_023523550.1 (LOW QUALITY PROTEIN: acyl-CoA--sterol O-acyltransferase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 485.7 bits (1249), Expect = 3.4e-133
Identity = 237/361 (65.65%), Postives = 284/361 (78.67%), Query Frame = 0


Query: 1   MENEIINFINVWLTVCASLCYCYLIGKNVRPGGRRLISIFPVVCLFLYLPLRLSSAHLGG 60
           ME EI+NFI VWLTVC SLCYC+  GK + PG  R +++ P+V LF  LPL+L+S HLGG
Sbjct: 1   MEGEILNFIKVWLTVCISLCYCHATGKLLPPGAGRFLAVAPIVVLFFLLPLQLTSIHLGG 60

Query: 61  PTAFFIAWLANFKLLLFSFAGGPLSSDPPLPLRRFLAVSCLPIKIQRNHLQIYQNGENPF 120
            T+FFIAWLANFKLLLF+   GPLS++ PLPLRRFLA+SCLPIKI +N            
Sbjct: 61  STSFFIAWLANFKLLLFAAGDGPLSAEQPLPLRRFLALSCLPIKILQNE----------- 120

Query: 121 PKSSPNAPQIETPLPSKSFLSYAIKGFLVALMVKLYDYSEFMHPKLVMCIYSWHIYFLLE 180
            KS PN  + +T L  KSFL+Y+IK  +VALMVKLYDYSEF+HPK+V+ IYSWH+YFLLE
Sbjct: 121 -KSCPNNNRSDTTLKPKSFLNYSIKAVIVALMVKLYDYSEFIHPKVVLFIYSWHLYFLLE 180

Query: 181 IILAAAKFLARNLLGAELEPQFNDPYFSTSLQDFWGRRWNLMVTGILNPTVYKPTIRLAA 240
           IILA  +F  RN+LG ELEPQF DPYFSTSLQDFWGRRWNLM T IL PTVYKP++++AA
Sbjct: 181 IILAITQFFGRNVLGIELEPQFQDPYFSTSLQDFWGRRWNLMATNILQPTVYKPSVKIAA 240

Query: 241 PVVGRKWAPLLAVLATFFVSAMMHELIFYYLGRMRPDWEVSRFFAVHGVALTLEILAKKA 300
            V+GRKWAPL AV+ATF VSAMMHELIFYY+GRMRP+WE + FF +HGVALT+EI+ KKA
Sbjct: 241 KVIGRKWAPLPAVMATFIVSAMMHELIFYYMGRMRPNWECTWFFVIHGVALTVEIMVKKA 300

Query: 301 WPEKCRRVPAVVSVPLTVGFVFVTGIWMFFPQFVRCGIDVKAFAEYAAVSDFFTKRLFLT 360
            PEK  R+P  VSVP+T+ F+F T IW+FFPQFVR  +DV+AF EYAA+  F  +R FL 
Sbjct: 301 LPEKW-RLPPAVSVPVTLAFIFPTAIWLFFPQFVRLQLDVRAFEEYAALXHFCKERRFLI 348

Query: 361 G 362
           G
Sbjct: 361 G 348

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BLAST of MS016073 vs. NCBI nr
Match: XP_022998466.1 (acyl-CoA--sterol O-acyltransferase 1-like [Cucurbita maxima])

HSP 1 Score: 481.5 bits (1238), Expect = 6.4e-132
Identity = 232/352 (65.91%), Postives = 280/352 (79.55%), Query Frame = 0


Query: 1   MENEIINFINVWLTVCASLCYCYLIGKNVRPGGRRLISIFPVVCLFLYLPLRLSSAHLGG 60
           ME EI+NFI VWLTVC SLCYC+  GK + PG  R +++ P+V LF +LPL+L+S HLGG
Sbjct: 1   MEGEILNFIKVWLTVCISLCYCHATGKLIPPGAGRFLAVAPIVVLFFFLPLQLTSIHLGG 60

Query: 61  PTAFFIAWLANFKLLLFSFAGGPLSSDPPLPLRRFLAVSCLPIKIQRNHLQIYQNGENPF 120
            T+FFIAWLANFKLLLF+   GPLS++PPLPLRRFLA+SCLPIKI +N            
Sbjct: 61  STSFFIAWLANFKLLLFTAGDGPLSAEPPLPLRRFLALSCLPIKILQNE----------- 120

Query: 121 PKSSPNAPQIETPLPSKSFLSYAIKGFLVALMVKLYDYSEFMHPKLVMCIYSWHIYFLLE 180
            KS PN  + +T L  KSFL+Y+IK  +VALMVKLYDYSEF+HPK+V+ IYSWH+YFLLE
Sbjct: 121 -KSCPNNNRSDTTLKPKSFLNYSIKAVIVALMVKLYDYSEFIHPKVVLFIYSWHLYFLLE 180

Query: 181 IILAAAKFLARNLLGAELEPQFNDPYFSTSLQDFWGRRWNLMVTGILNPTVYKPTIRLAA 240
           IILA  +F  RN+LG ELEPQF DPYFSTSLQDFWGRRWNLM T IL PTVYKP++++AA
Sbjct: 181 IILAITQFFGRNVLGIELEPQFQDPYFSTSLQDFWGRRWNLMATNILQPTVYKPSVKIAA 240

Query: 241 PVVGRKWAPLLAVLATFFVSAMMHELIFYYLGRMRPDWEVSRFFAVHGVALTLEILAKKA 300
            V+GRKWAPL AV+ATF VSAMMHELIF+Y+GRMRP+WE + FF +HGVALT+EI+ KK 
Sbjct: 241 KVIGRKWAPLPAVMATFLVSAMMHELIFFYMGRMRPNWECTWFFVIHGVALTVEIMVKKV 300

Query: 301 WPEKCRRVPAVVSVPLTVGFVFVTGIWMFFPQFVRCGIDVKAFAEYAAVSDF 353
            PEK  R+P  VSVP+T+ F+F T IW+FFPQFVR  +DV+AF EYAA+  F
Sbjct: 301 LPEKW-RLPPAVSVPVTLAFIFPTAIWLFFPQFVRLKLDVRAFEEYAALVTF 339

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BLAST of MS016073 vs. NCBI nr
Match: XP_022948970.1 (acyl-CoA--sterol O-acyltransferase 1-like [Cucurbita moschata] >KAG6606895.1 Acyl-CoA--sterol O-acyltransferase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 480.3 bits (1235), Expect = 1.4e-131
Identity = 233/352 (66.19%), Postives = 279/352 (79.26%), Query Frame = 0


Query: 1   MENEIINFINVWLTVCASLCYCYLIGKNVRPGGRRLISIFPVVCLFLYLPLRLSSAHLGG 60
           ME EI+NFI VWLTVC SLCYC+  GK + PG  R +++ P+V LF  LPL+L+S HLGG
Sbjct: 1   MEGEILNFIKVWLTVCISLCYCHATGKLLAPGPGRFLAVAPIVVLFFLLPLQLTSIHLGG 60

Query: 61  PTAFFIAWLANFKLLLFSFAGGPLSSDPPLPLRRFLAVSCLPIKIQRNHLQIYQNGENPF 120
            T+FFIAWLANFKLLLF+   GPLS++PPLPLRRFLA+SCLPIKI +N            
Sbjct: 61  STSFFIAWLANFKLLLFAAGDGPLSAEPPLPLRRFLALSCLPIKILQNE----------- 120

Query: 121 PKSSPNAPQIETPLPSKSFLSYAIKGFLVALMVKLYDYSEFMHPKLVMCIYSWHIYFLLE 180
            KS PN  + +T L  KSFL+Y+IK  +VALMVKLYDYSEF+HPK+V+ IYSWH+YFLLE
Sbjct: 121 -KSCPNNNRSDTTLKPKSFLNYSIKAVIVALMVKLYDYSEFIHPKVVLFIYSWHLYFLLE 180

Query: 181 IILAAAKFLARNLLGAELEPQFNDPYFSTSLQDFWGRRWNLMVTGILNPTVYKPTIRLAA 240
           IILA  +F  RN+LG ELEPQF DPYFSTSLQDFWGRRWNLM T IL PTVYKP++++AA
Sbjct: 181 IILAITQFFGRNVLGIELEPQFQDPYFSTSLQDFWGRRWNLMATNILQPTVYKPSVKIAA 240

Query: 241 PVVGRKWAPLLAVLATFFVSAMMHELIFYYLGRMRPDWEVSRFFAVHGVALTLEILAKKA 300
            V+GRKWAPL AV+ATF VSAMMHELIFYY+GRMRP+WE + FF +HGVALT+EI+ KK 
Sbjct: 241 KVIGRKWAPLPAVMATFLVSAMMHELIFYYMGRMRPNWECTWFFVIHGVALTVEIMVKKV 300

Query: 301 WPEKCRRVPAVVSVPLTVGFVFVTGIWMFFPQFVRCGIDVKAFAEYAAVSDF 353
            PEK  R+P  VSVP+T+ F+F T IW+FFPQFVR  +DV+AF EYAA+  F
Sbjct: 301 LPEKW-RLPPAVSVPVTLAFIFPTAIWLFFPQFVRLKLDVRAFEEYAALVTF 339

[Close]

BLAST of MS016073 vs. NCBI nr
Match: XP_022934183.1 (acyl-CoA--sterol O-acyltransferase 1-like [Cucurbita moschata])

HSP 1 Score: 474.6 bits (1220), Expect = 7.8e-130
Identity = 235/358 (65.64%), Postives = 274/358 (76.54%), Query Frame = 0


Query: 1   MENEIINFINVWLTVCASLCYCYLIGKNVRPGGRRLISIFPVVCLFLYLPLRLSSAHLGG 60
           ME E+INFI VWL VC SLCYC+  GK + PG  R ++I PVV LF  LPL+L+S HLGG
Sbjct: 1   MEGEVINFIMVWLIVCMSLCYCHTAGKILPPGAGRFLAIAPVVVLFFLLPLQLTSVHLGG 60

Query: 61  PTAFFIAWLANFKLLLFSFAGGPLSSDPPLPLRRFLAVSCLPIKIQRNHLQIYQNGENPF 120
            TAFFIAWLANFKLLLF+   GPLS+ PPLPLRRFLA+SCLPIKI           +NP 
Sbjct: 61  STAFFIAWLANFKLLLFTAGNGPLSATPPLPLRRFLALSCLPIKIL----------QNPS 120

Query: 121 PKSSPNAPQIETPLPSKSFLSYAIKGFLVALMVKLYDYSEFMHPKLVMCIYSWHIYFLLE 180
            K SP           KSFL+Y IK  +VALMVKLYDYSEF+HPK+++ IYSWH+YF LE
Sbjct: 121 EKQSPKNHHETLNQEPKSFLNYWIKALIVALMVKLYDYSEFIHPKVILFIYSWHVYFFLE 180

Query: 181 IILAAAKFLARNLLGAELEPQFNDPYFSTSLQDFWGRRWNLMVTGILNPTVYKPTIRLAA 240
           IILA  +F  R LLG ELEPQF+DPYFSTSLQDFWGRRWNLM T IL PT+YKPT+++AA
Sbjct: 181 IILAITQFFGRTLLGIELEPQFHDPYFSTSLQDFWGRRWNLMATNILQPTIYKPTVKIAA 240

Query: 241 PVVGRKWAPLLAVLATFFVSAMMHELIFYYLGRMRPDWEVSRFFAVHGVALTLEILAKKA 300
            VVGRKWAPL  V+ATF VSA+MHELIFYYLGRM+P+WE++ FF +HG ALT+EIL KK 
Sbjct: 241 KVVGRKWAPLPGVMATFLVSAIMHELIFYYLGRMKPNWELTWFFVIHGFALTVEILVKKV 300

Query: 301 WPEKCRRVPAVVSVPLTVGFVFVTGIWMFFPQFVRCGIDVKAFAEYAAVSDFFTKRLF 359
            PEK RR+PA VSVP+T+ F+F T IW+FFPQF+R  IDVKAF EYAA+  F  K  F
Sbjct: 301 LPEK-RRLPAAVSVPVTLVFIFSTAIWLFFPQFMRLKIDVKAFEEYAALVSFIKKLAF 347

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BLAST of MS016073 vs. ExPASy Swiss-Prot
Match: Q9SV07 (Acyl-CoA--sterol O-acyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=ASAT1 PE=1 SV=1)

HSP 1 Score: 312.4 bits (799), Expect = 6.8e-84
Identity = 176/348 (50.57%), Postives = 222/348 (63.79%), Query Frame = 0


Query: 7   NFINVWLTVCASLCYCYLIGKNVRPGGRRLISIFPVVCLFLYLPLRLSSAHLGGPTAFFI 66
           +FI  W  V  SLCY + I K V  G +RLI  FPV  +F  +P  + S HL G TAFFI
Sbjct: 3   SFIKAWGLVIISLCYTFFIAKLVPKGIKRLILFFPVFLIFFIVPFLIYSLHLLGITAFFI 62

Query: 67  AWLANFKLLLFSFAGGPLSSD-PPLPLRRFLAVSCLPIKIQRNHLQIYQNGENPFP-KSS 126
           AWLANFKLLLF+   GPLSS+  PL L  FLAVSCLPIKIQ           +P P K+ 
Sbjct: 63  AWLANFKLLLFALGRGPLSSNHKPLSLPIFLAVSCLPIKIQL----------SPKPTKTH 122

Query: 127 PNAPQIETPLPSKSFLSYAIKGFLVALMVKLYDYSEFMHPKLVMCIYSWHIYFLLEIILA 186
            +    E P      L Y IK   V L++K Y+YS  +  K+V+ +Y+ HIYF LEIILA
Sbjct: 123 SHEGSTEGP------LIYTIKAVFVVLIIKAYEYSTKLPEKVVLTLYAIHIYFALEIILA 182

Query: 187 AAKFLARNLLGAELEPQFNDPYFSTSLQDFWGRRWNLMVTGILNPTVYKPTIRLAAPVVG 246
           A     R +   ELEPQFN PY +TSLQDFWGRRWNLMVTGIL PTVY+P+++L + V+G
Sbjct: 183 ATAAAVRAMSDLELEPQFNKPYLATSLQDFWGRRWNLMVTGILRPTVYEPSLQLFS-VLG 242

Query: 247 RKWAPLLAVLATFFVSAMMHELIFYYLGRMRPDWEVSRFFAVHGVALTLEILAKKAWPEK 306
             ++ +LA   TF VS +MHELIF+Y+GR+RPDW++  FF ++G   T+EI  KK    +
Sbjct: 243 PNYSQILAAFGTFVVSGIMHELIFFYMGRLRPDWKMMWFFLINGFCTTVEIAIKKTINGR 302

Query: 307 CRRVPAVVSVPLTVGFVFVTGIWMFFPQFVRCGIDVKAFAEYAAVSDF 353
             R P  +S  LT+ FV VT +W+F P+F RC I  KA  EYAA+  F
Sbjct: 303 W-RFPKAISQVLTLTFVMVTALWLFLPEFNRCNIVEKALDEYAAIGAF 332

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BLAST of MS016073 vs. ExPASy Swiss-Prot
Match: Q9XGY6 (Long-chain-alcohol O-fatty-acyltransferase OS=Simmondsia chinensis OX=3999 PE=1 SV=1)

HSP 1 Score: 280.8 bits (717), Expect = 2.2e-74
Identity = 154/358 (43.02%), Postives = 216/358 (60.34%), Query Frame = 0


Query: 1   MENEIINFINVWLTVCASLCYCYLIGKNVRPGGR-RLISIFPVVCLFLYLPLRLSSAHLG 60
           +E E+  F  VW++  A+ CYC  +      GG  RL+ + PVV LF++LPLRLSS HLG
Sbjct: 3   VEKELKTFSEVWISAIAAACYCRFVPAVAPHGGALRLLLLLPVVLLFIFLPLRLSSFHLG 62

Query: 61  GPTAFFIAWLANFKLLLFSFAGGPLSSDPPLPLRRFLAVSCLPIKIQRNHLQIYQNGENP 120
           GPTA ++ WLANFKLLLF+F  GPL S+P L L  F++ + LPIK + +           
Sbjct: 63  GPTALYLVWLANFKLLLFAFHLGPL-SNPSLSLLHFISTTLLPIKFRDD----------- 122

Query: 121 FPKSSPNAPQIETPLPSKSFLSYAIKGFLVALMVKLYDYSEFMHPKLVMCIYSWHIYFLL 180
            P +     +       +  + +  K    A ++K+Y++ + +   ++  +Y +H Y   
Sbjct: 123 -PSNDHEKNKRTLSFEWRKVVLFVAKLVFFAGILKIYEFRKDLPHFVISVLYCFHFYLGT 182

Query: 181 EIILAAAKFLARNLLGAELEPQFNDPYFSTSLQDFWGRRWNLMVTGILNPTVYKPTIRLA 240
           EI LAA+  +AR  LG +L PQFN+PY +TSLQDFWGRRWNLMV+ IL  T Y+P  R+ 
Sbjct: 183 EITLAASAVIARATLGLDLYPQFNEPYLATSLQDFWGRRWNLMVSDILGLTTYQPVRRVL 242

Query: 241 APVVGRKWAPLLAVLATFFVSAMMHELIFYYLGRMRPDWEVSRFFAVHGVALTLEILAKK 300
           +  V  +W    A+L  F VS +MHE+ F+YL R RP WEV+ FF +HGV   +E++ KK
Sbjct: 243 SRWVRLRWEVAGAMLVAFTVSGLMHEVFFFYLTRARPSWEVTGFFVLHGVCTAVEMVVKK 302

Query: 301 AWPEKCRRVPAVVSVPLTVGFVFVTGIWMFFPQFVRCGIDVKAFAEYAAVSDFFTKRL 358
           A   K  R+   VS  LTVGFV VTG W+F PQ VR G+D+K   EY  + ++  K+L
Sbjct: 303 AVSGKV-RLRREVSGALTVGFVMVTGGWLFLPQLVRHGVDLKTIDEYPVMFNYTQKKL 346

[Close]

BLAST of MS016073 vs. ExPASy Swiss-Prot
Match: Q9FJ75 (Probable long-chain-alcohol O-fatty-acyltransferase 4 OS=Arabidopsis thaliana OX=3702 GN=AT4 PE=2 SV=1)

HSP 1 Score: 271.2 bits (692), Expect = 1.7e-71
Identity = 152/358 (42.46%), Postives = 206/358 (57.54%), Query Frame = 0


Query: 1   MENEIINFINVWLTVCASLCYCYLIGKNVRPGGRRLISIFPVVCLFLYLPLRLSSAHLGG 60
           ME E+++ I VW+    S+ YCY     ++ G  RL+S+ PV  LFL LPL +SS H  G
Sbjct: 1   MEEELMSLIKVWVYAIISISYCYYTSTRIKSGVFRLLSVLPVCVLFLVLPLFVSSVHFSG 60

Query: 61  PTAFFIAWLANFKLLLFSFAGGPLSSDPPLPLRRFLAVSCLPIKIQRNHLQIYQNGENPF 120
            TAFF++WLANFKL+LFSF  GPL    P  L RF+  +C PIK+Q+N            
Sbjct: 61  STAFFLSWLANFKLILFSFDQGPL-FPVPSNLSRFVCFTCFPIKLQQN------------ 120

Query: 121 PKSSPNAPQIETPLPSKSFLSYAIKGFLVALMVKLYDYSEFMHPKLVMCIYSWHIYFLLE 180
                  P+ +  +P   F   A+K     +++ +Y+Y + M P +++ +YS HIY   E
Sbjct: 121 -------PKPQNQMPKWGF---AVKLAFFGVLLHMYEYKQHMSPTVLLVLYSLHIYLEYE 180

Query: 181 IILAAAKFLARNLLGAELEPQFNDPYFSTSLQDFWGRRWNLMVTGILNPTVYKPTIRLAA 240
           I+LA  K L    L  +LEP FN+PY STSLQDFWGRRWNLMV  IL P VY P  ++A 
Sbjct: 181 ILLAPLKVLLSISLWCDLEPHFNEPYLSTSLQDFWGRRWNLMVPAILRPAVYLPVRQMAG 240

Query: 241 PVVGRKWAPLLAVLATFFVSAMMHELIFYYLGRMRPDWEVSRFFAVHGVALTLEILAKKA 300
             +    A  L V A+F VS ++HELIF+Y  R  P  EV+ FF +HGV    E  AK+ 
Sbjct: 241 RKMNSDQALFLGVFASFLVSGVVHELIFFYFTRESPTGEVTLFFVLHGVCTAAECAAKRT 300

Query: 301 WPEKCRRVPAVVSVPLTVGFVFVTGIWMFFPQFVRCGIDVKAFAEYAAVSDFFTKRLF 359
              +  +V  +VS  LTVGFV +TG W+FFP   R G+ ++  A+ A +   F K  F
Sbjct: 301 RLVRRWKVSQMVSRLLTVGFVVMTGGWLFFPHLARSGM-IERLADEAFLFIGFVKHKF 334

[Close]

BLAST of MS016073 vs. ExPASy Swiss-Prot
Match: Q9FJ76 (Probable long-chain-alcohol O-fatty-acyltransferase 5 OS=Arabidopsis thaliana OX=3702 GN=AT5 PE=2 SV=1)

HSP 1 Score: 269.2 bits (687), Expect = 6.6e-71
Identity = 156/359 (43.45%), Postives = 208/359 (57.94%), Query Frame = 0


Query: 1   MENEIINFINVWLTVCASLCYCYLIGKNVRPGGRRLISIFPVVCLFLYLPLRLSSAHLGG 60
           M+ E+ N I VW++   S+ YCY I   ++ G  R +S+ PV+ LFL LPL  SS HL  
Sbjct: 1   MDEELKNLIKVWVSAIISISYCYYIPPRIKSGAPRFLSVSPVLALFLVLPLFFSSLHLSL 60

Query: 61  PTAFFIAWLANFKLLLFSFAGGPLSSDPPLPLR--RFLAVSCLPIKIQRNHLQIYQNGEN 120
            TAFF+ WLANFKL+LFSF  GPL    P+P    RF   +C PIK+Q+N          
Sbjct: 61  ITAFFLTWLANFKLILFSFDKGPLI---PIPTNFPRFFCFTCFPIKVQQN---------- 120

Query: 121 PFPKSSPNAPQIETPLPSKSFLSYAIKGFLVALMVKLYDYSEFMHPKLVMCIYSWHIYFL 180
             PKS  + P+          L +AIK  + A+++ LY Y + + P +++ +Y  H+Y  
Sbjct: 121 --PKSQNHLPK----------LVFAIKLAIFAVLLHLYSYRQNLSPTILLGLYFVHLYLE 180

Query: 181 LEIILAAAKFLARNLLGAELEPQFNDPYFSTSLQDFWGRRWNLMVTGILNPTVYKPTIRL 240
           +EIIL   K +    LG +LEPQ N PY +TSLQDFWGRRWNLMV  IL P VY P  R+
Sbjct: 181 IEIILTFVKVVVFISLGCDLEPQSNKPYLATSLQDFWGRRWNLMVPAILRPAVYAPMRRV 240

Query: 241 AAPVVGRKWAPLLAVLATFFVSAMMHELIFYYLGRMRPDWEVSRFFAVHGVALTLEILAK 300
           +   +   WA    +LA F VS ++HEL+F+YL R  P  EV+ FF +HGV   +E+  K
Sbjct: 241 SERKMSSGWALFPGILAAFIVSGLVHELLFFYLIREMPTGEVTLFFVLHGVCTAVELAVK 300

Query: 301 KAWPEKCR-RVPAVVSVPLTVGFVFVTGIWMFFPQFVRCGIDVKAFAEYAAVSDFFTKR 357
           K      R R+   VS  LTVGFVFVTG W+F PQ  R G+ ++ F   A +   F KR
Sbjct: 301 KKTTVAQRWRLSPGVSRVLTVGFVFVTGGWLFTPQLKRSGV-MERFTSEAVLLVEFIKR 333

[Close]

BLAST of MS016073 vs. ExPASy Swiss-Prot
Match: Q9FJ74 (Probable long-chain-alcohol O-fatty-acyltransferase 3 OS=Arabidopsis thaliana OX=3702 GN=AT3 PE=3 SV=1)

HSP 1 Score: 267.3 bits (682), Expect = 2.5e-70
Identity = 154/362 (42.54%), Postives = 207/362 (57.18%), Query Frame = 0


Query: 1   MENEIINFINVWLTVCASLCYCYLIGKNVRPGGRRLISIFPVVCLFLYLPLRLSSAHLGG 60
           ME E+ NFI +W++   S+ YCY +   ++ G  RL+S+ PV  LFL  PL  S  H  G
Sbjct: 1   MEEELKNFIKLWISAIISISYCYYLSTGIKAGVFRLLSVLPVCALFLVFPLFFSYVHFSG 60

Query: 61  PTAFFIAWLANFKLLLFSFAGGPLSSDPPLP--LRRFLAVSCLPIKIQRNHLQIYQNGEN 120
             AFF++WLANFKL+LFSF  GPLS   PLP  L RF+ ++C PIK              
Sbjct: 61  CMAFFLSWLANFKLILFSFDQGPLS---PLPRTLSRFICITCFPIK-------------- 120

Query: 121 PFPKSSPNAPQIETPLPSKSFLSYAIKGFLVALMVKLYDYSEFMHPKLVMCIYSWHIYFL 180
             P+ +PN    + P+       +AIK  +  +++++Y+Y +++ P L++   S HI+  
Sbjct: 121 --PQQNPNIQNYKIPI-----WLFAIKVVIFVVLLQMYEYKQYLSPALLLVFNSLHIFLE 180

Query: 181 LEIILAAAKFLARNLLGAELEPQFNDPYFSTSLQDFWGRRWNLMVTGILNPTVYKPTIRL 240
           LEI+    K L    LG +LEPQ N+PY +TSLQDFWGRRWNLMV  IL P VY P  R+
Sbjct: 181 LEIVFMLVKALVFITLGCDLEPQSNEPYLATSLQDFWGRRWNLMVPAILRPAVYLPARRM 240

Query: 241 AAPVVGRKWAPLLAVLATFFVSAMMHELIFYYLGRMRPDWEVSRFFAVHGVALTLEILAK 300
           A   V    A  L V A F VS  +HE++F+YL R  P  EV+ FF +HGV    E+  K
Sbjct: 241 ACRKVNSDQAMFLGVFAAFLVSGAVHEMLFFYLTREVPTGEVTWFFLLHGVCTVAEVAVK 300

Query: 301 KA-WPEKCRRVPAVVSVPLTVGFVFVTGIWMFFPQFVRCGIDVKAFAEYAAVSDFFTKRL 360
           K+ +  +  RV   VS  LTVGFV VT  W FFP  +R GI ++  A  A +   F K  
Sbjct: 301 KSTFVRRWWRVSPTVSRLLTVGFVVVTSGWFFFP-LIRSGI-IERLASEALMCIDFVKHK 336

[Close]

BLAST of MS016073 vs. ExPASy TrEMBL
Match: A0A6J1DGQ4 (acyl-CoA--sterol O-acyltransferase 1-like OS=Momordica charantia OX=3673 GN=LOC111020333 PE=3 SV=1)

HSP 1 Score: 724.5 bits (1869), Expect = 2.1e-205
Identity = 356/361 (98.61%), Postives = 357/361 (98.89%), Query Frame = 0


Query: 1   MENEIINFINVWLTVCASLCYCYLIGKNVRPGGRRLISIFPVVCLFLYLPLRLSSAHLGG 60
           MENEIINFINVWLTVCASLCYCYLIGKNVRPGGRRLISIFPVVCLFLYLPLRLSSAHLGG
Sbjct: 1   MENEIINFINVWLTVCASLCYCYLIGKNVRPGGRRLISIFPVVCLFLYLPLRLSSAHLGG 60

Query: 61  PTAFFIAWLANFKLLLFSFAGGPLSSDPPLPLRRFLAVSCLPIKIQRNHLQIYQNGENPF 120
           PTAFFIAWLANFKLLLFSFAGGPLSSDPPLPLRRFLAVSCLPIKIQRNHLQI QNGEN  
Sbjct: 61  PTAFFIAWLANFKLLLFSFAGGPLSSDPPLPLRRFLAVSCLPIKIQRNHLQISQNGENTS 120

Query: 121 PKSSPNAPQIETPLPSKSFLSYAIKGFLVALMVKLYDYSEFMHPKLVMCIYSWHIYFLLE 180
           PKSSPNAPQIETPLPSKSFLSYAIKGFLVALMVKLYDYSEFMHPKLVMCIYSWHIYFLLE
Sbjct: 121 PKSSPNAPQIETPLPSKSFLSYAIKGFLVALMVKLYDYSEFMHPKLVMCIYSWHIYFLLE 180

Query: 181 IILAAAKFLARNLLGAELEPQFNDPYFSTSLQDFWGRRWNLMVTGILNPTVYKPTIRLAA 240
           IILAAAKFLARNLLGAELEPQFNDPYFSTSLQDFWGRRWNLMVTGILNPTVYKPTIRLAA
Sbjct: 181 IILAAAKFLARNLLGAELEPQFNDPYFSTSLQDFWGRRWNLMVTGILNPTVYKPTIRLAA 240

Query: 241 PVVGRKWAPLLAVLATFFVSAMMHELIFYYLGRMRPDWEVSRFFAVHGVALTLEILAKKA 300
           PVVGRKWAPLLAVLATFFVSAMMHELIFYYLGRMRPDWEVSRFFAVHGVALT+EILAKKA
Sbjct: 241 PVVGRKWAPLLAVLATFFVSAMMHELIFYYLGRMRPDWEVSRFFAVHGVALTVEILAKKA 300

Query: 301 WPEKCRRVPAVVSVPLTVGFVFVTGIWMFFPQFVRCGIDVKAFAEYAAVSDFFTKRLFLT 360
           WPEKCRRVPAVVSVPLTVGFVFVTGIWMFFPQFVRCGIDVKAFAEYAAVSDFFTKRLFL 
Sbjct: 301 WPEKCRRVPAVVSVPLTVGFVFVTGIWMFFPQFVRCGIDVKAFAEYAAVSDFFTKRLFLI 360

Query: 361 G 362
           G
Sbjct: 361 G 361

[Close]

BLAST of MS016073 vs. ExPASy TrEMBL
Match: A0A6J1KAA2 (acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111493091 PE=3 SV=1)

HSP 1 Score: 481.5 bits (1238), Expect = 3.1e-132
Identity = 232/352 (65.91%), Postives = 280/352 (79.55%), Query Frame = 0


Query: 1   MENEIINFINVWLTVCASLCYCYLIGKNVRPGGRRLISIFPVVCLFLYLPLRLSSAHLGG 60
           ME EI+NFI VWLTVC SLCYC+  GK + PG  R +++ P+V LF +LPL+L+S HLGG
Sbjct: 1   MEGEILNFIKVWLTVCISLCYCHATGKLIPPGAGRFLAVAPIVVLFFFLPLQLTSIHLGG 60

Query: 61  PTAFFIAWLANFKLLLFSFAGGPLSSDPPLPLRRFLAVSCLPIKIQRNHLQIYQNGENPF 120
            T+FFIAWLANFKLLLF+   GPLS++PPLPLRRFLA+SCLPIKI +N            
Sbjct: 61  STSFFIAWLANFKLLLFTAGDGPLSAEPPLPLRRFLALSCLPIKILQNE----------- 120

Query: 121 PKSSPNAPQIETPLPSKSFLSYAIKGFLVALMVKLYDYSEFMHPKLVMCIYSWHIYFLLE 180
            KS PN  + +T L  KSFL+Y+IK  +VALMVKLYDYSEF+HPK+V+ IYSWH+YFLLE
Sbjct: 121 -KSCPNNNRSDTTLKPKSFLNYSIKAVIVALMVKLYDYSEFIHPKVVLFIYSWHLYFLLE 180

Query: 181 IILAAAKFLARNLLGAELEPQFNDPYFSTSLQDFWGRRWNLMVTGILNPTVYKPTIRLAA 240
           IILA  +F  RN+LG ELEPQF DPYFSTSLQDFWGRRWNLM T IL PTVYKP++++AA
Sbjct: 181 IILAITQFFGRNVLGIELEPQFQDPYFSTSLQDFWGRRWNLMATNILQPTVYKPSVKIAA 240

Query: 241 PVVGRKWAPLLAVLATFFVSAMMHELIFYYLGRMRPDWEVSRFFAVHGVALTLEILAKKA 300
            V+GRKWAPL AV+ATF VSAMMHELIF+Y+GRMRP+WE + FF +HGVALT+EI+ KK 
Sbjct: 241 KVIGRKWAPLPAVMATFLVSAMMHELIFFYMGRMRPNWECTWFFVIHGVALTVEIMVKKV 300

Query: 301 WPEKCRRVPAVVSVPLTVGFVFVTGIWMFFPQFVRCGIDVKAFAEYAAVSDF 353
            PEK  R+P  VSVP+T+ F+F T IW+FFPQFVR  +DV+AF EYAA+  F
Sbjct: 301 LPEKW-RLPPAVSVPVTLAFIFPTAIWLFFPQFVRLKLDVRAFEEYAALVTF 339

[Close]

BLAST of MS016073 vs. ExPASy TrEMBL
Match: A0A6J1GBE5 (acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111452458 PE=3 SV=1)

HSP 1 Score: 480.3 bits (1235), Expect = 6.9e-132
Identity = 233/352 (66.19%), Postives = 279/352 (79.26%), Query Frame = 0


Query: 1   MENEIINFINVWLTVCASLCYCYLIGKNVRPGGRRLISIFPVVCLFLYLPLRLSSAHLGG 60
           ME EI+NFI VWLTVC SLCYC+  GK + PG  R +++ P+V LF  LPL+L+S HLGG
Sbjct: 1   MEGEILNFIKVWLTVCISLCYCHATGKLLAPGPGRFLAVAPIVVLFFLLPLQLTSIHLGG 60

Query: 61  PTAFFIAWLANFKLLLFSFAGGPLSSDPPLPLRRFLAVSCLPIKIQRNHLQIYQNGENPF 120
            T+FFIAWLANFKLLLF+   GPLS++PPLPLRRFLA+SCLPIKI +N            
Sbjct: 61  STSFFIAWLANFKLLLFAAGDGPLSAEPPLPLRRFLALSCLPIKILQNE----------- 120

Query: 121 PKSSPNAPQIETPLPSKSFLSYAIKGFLVALMVKLYDYSEFMHPKLVMCIYSWHIYFLLE 180
            KS PN  + +T L  KSFL+Y+IK  +VALMVKLYDYSEF+HPK+V+ IYSWH+YFLLE
Sbjct: 121 -KSCPNNNRSDTTLKPKSFLNYSIKAVIVALMVKLYDYSEFIHPKVVLFIYSWHLYFLLE 180

Query: 181 IILAAAKFLARNLLGAELEPQFNDPYFSTSLQDFWGRRWNLMVTGILNPTVYKPTIRLAA 240
           IILA  +F  RN+LG ELEPQF DPYFSTSLQDFWGRRWNLM T IL PTVYKP++++AA
Sbjct: 181 IILAITQFFGRNVLGIELEPQFQDPYFSTSLQDFWGRRWNLMATNILQPTVYKPSVKIAA 240

Query: 241 PVVGRKWAPLLAVLATFFVSAMMHELIFYYLGRMRPDWEVSRFFAVHGVALTLEILAKKA 300
            V+GRKWAPL AV+ATF VSAMMHELIFYY+GRMRP+WE + FF +HGVALT+EI+ KK 
Sbjct: 241 KVIGRKWAPLPAVMATFLVSAMMHELIFYYMGRMRPNWECTWFFVIHGVALTVEIMVKKV 300

Query: 301 WPEKCRRVPAVVSVPLTVGFVFVTGIWMFFPQFVRCGIDVKAFAEYAAVSDF 353
            PEK  R+P  VSVP+T+ F+F T IW+FFPQFVR  +DV+AF EYAA+  F
Sbjct: 301 LPEKW-RLPPAVSVPVTLAFIFPTAIWLFFPQFVRLKLDVRAFEEYAALVTF 339

[Close]

BLAST of MS016073 vs. ExPASy TrEMBL
Match: A0A6J1F6Y6 (acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111441427 PE=3 SV=1)

HSP 1 Score: 474.6 bits (1220), Expect = 3.8e-130
Identity = 235/358 (65.64%), Postives = 274/358 (76.54%), Query Frame = 0


Query: 1   MENEIINFINVWLTVCASLCYCYLIGKNVRPGGRRLISIFPVVCLFLYLPLRLSSAHLGG 60
           ME E+INFI VWL VC SLCYC+  GK + PG  R ++I PVV LF  LPL+L+S HLGG
Sbjct: 1   MEGEVINFIMVWLIVCMSLCYCHTAGKILPPGAGRFLAIAPVVVLFFLLPLQLTSVHLGG 60

Query: 61  PTAFFIAWLANFKLLLFSFAGGPLSSDPPLPLRRFLAVSCLPIKIQRNHLQIYQNGENPF 120
            TAFFIAWLANFKLLLF+   GPLS+ PPLPLRRFLA+SCLPIKI           +NP 
Sbjct: 61  STAFFIAWLANFKLLLFTAGNGPLSATPPLPLRRFLALSCLPIKIL----------QNPS 120

Query: 121 PKSSPNAPQIETPLPSKSFLSYAIKGFLVALMVKLYDYSEFMHPKLVMCIYSWHIYFLLE 180
            K SP           KSFL+Y IK  +VALMVKLYDYSEF+HPK+++ IYSWH+YF LE
Sbjct: 121 EKQSPKNHHETLNQEPKSFLNYWIKALIVALMVKLYDYSEFIHPKVILFIYSWHVYFFLE 180

Query: 181 IILAAAKFLARNLLGAELEPQFNDPYFSTSLQDFWGRRWNLMVTGILNPTVYKPTIRLAA 240
           IILA  +F  R LLG ELEPQF+DPYFSTSLQDFWGRRWNLM T IL PT+YKPT+++AA
Sbjct: 181 IILAITQFFGRTLLGIELEPQFHDPYFSTSLQDFWGRRWNLMATNILQPTIYKPTVKIAA 240

Query: 241 PVVGRKWAPLLAVLATFFVSAMMHELIFYYLGRMRPDWEVSRFFAVHGVALTLEILAKKA 300
            VVGRKWAPL  V+ATF VSA+MHELIFYYLGRM+P+WE++ FF +HG ALT+EIL KK 
Sbjct: 241 KVVGRKWAPLPGVMATFLVSAIMHELIFYYLGRMKPNWELTWFFVIHGFALTVEILVKKV 300

Query: 301 WPEKCRRVPAVVSVPLTVGFVFVTGIWMFFPQFVRCGIDVKAFAEYAAVSDFFTKRLF 359
            PEK RR+PA VSVP+T+ F+F T IW+FFPQF+R  IDVKAF EYAA+  F  K  F
Sbjct: 301 LPEK-RRLPAAVSVPVTLVFIFSTAIWLFFPQFMRLKIDVKAFEEYAALVSFIKKLAF 347

[Close]

BLAST of MS016073 vs. ExPASy TrEMBL
Match: A0A6J1J5C4 (acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111481453 PE=3 SV=1)

HSP 1 Score: 474.2 bits (1219), Expect = 5.0e-130
Identity = 236/358 (65.92%), Postives = 273/358 (76.26%), Query Frame = 0


Query: 1   MENEIINFINVWLTVCASLCYCYLIGKNVRPGGRRLISIFPVVCLFLYLPLRLSSAHLGG 60
           ME EIINF  VWL VC SLCYC+  GK + PG  R ++I PVV LF  LPL+L+S HLGG
Sbjct: 1   MEGEIINFTMVWLIVCMSLCYCHTAGKILHPGAGRFLAISPVVLLFFLLPLQLTSVHLGG 60

Query: 61  PTAFFIAWLANFKLLLFSFAGGPLSSDPPLPLRRFLAVSCLPIKIQRNHLQIYQNGENPF 120
            TAFFIAWLANFKLLLF+   GPLS+ PPLPLRRFLA+SCLPIKI           +NP 
Sbjct: 61  ATAFFIAWLANFKLLLFAAGNGPLSATPPLPLRRFLALSCLPIKIL----------QNPS 120

Query: 121 PKSSPNAPQIETPLPSKSFLSYAIKGFLVALMVKLYDYSEFMHPKLVMCIYSWHIYFLLE 180
            K SP           KSFL+Y IK  +VALMVKLYDYSEF+HPK+++ IYSWH+YF LE
Sbjct: 121 EKQSPKNHHETLNQEPKSFLNYWIKALIVALMVKLYDYSEFIHPKVILFIYSWHVYFFLE 180

Query: 181 IILAAAKFLARNLLGAELEPQFNDPYFSTSLQDFWGRRWNLMVTGILNPTVYKPTIRLAA 240
           IILA  +F  R LLG ELEPQF+DPYFSTSLQDFWGRRWNLM T IL PTVYKPT+++AA
Sbjct: 181 IILAITQFFGRTLLGIELEPQFHDPYFSTSLQDFWGRRWNLMATNILQPTVYKPTVKIAA 240

Query: 241 PVVGRKWAPLLAVLATFFVSAMMHELIFYYLGRMRPDWEVSRFFAVHGVALTLEILAKKA 300
            VVGRKWAPL  V+ATF VSA+MHELIFYYLGRM+P+WE++ FF +HG ALT+EIL KK 
Sbjct: 241 KVVGRKWAPLPGVMATFLVSAIMHELIFYYLGRMKPNWELTWFFVIHGFALTVEILVKKV 300

Query: 301 WPEKCRRVPAVVSVPLTVGFVFVTGIWMFFPQFVRCGIDVKAFAEYAAVSDFFTKRLF 359
            PEK RR+PA VSVP+T+ F+F T IW+FFPQF+R  IDVKAF EYAA+  F  K  F
Sbjct: 301 LPEK-RRLPAAVSVPVTLVFIFSTAIWLFFPQFMRLKIDVKAFEEYAALVSFMKKLAF 347

[Close]

BLAST of MS016073 vs. TAIR 10
Match: AT3G51970.1 (acyl-CoA sterol acyl transferase 1 )

HSP 1 Score: 312.4 bits (799), Expect = 4.8e-85
Identity = 176/348 (50.57%), Postives = 222/348 (63.79%), Query Frame = 0


Query: 7   NFINVWLTVCASLCYCYLIGKNVRPGGRRLISIFPVVCLFLYLPLRLSSAHLGGPTAFFI 66
           +FI  W  V  SLCY + I K V  G +RLI  FPV  +F  +P  + S HL G TAFFI
Sbjct: 3   SFIKAWGLVIISLCYTFFIAKLVPKGIKRLILFFPVFLIFFIVPFLIYSLHLLGITAFFI 62

Query: 67  AWLANFKLLLFSFAGGPLSSD-PPLPLRRFLAVSCLPIKIQRNHLQIYQNGENPFP-KSS 126
           AWLANFKLLLF+   GPLSS+  PL L  FLAVSCLPIKIQ           +P P K+ 
Sbjct: 63  AWLANFKLLLFALGRGPLSSNHKPLSLPIFLAVSCLPIKIQL----------SPKPTKTH 122

Query: 127 PNAPQIETPLPSKSFLSYAIKGFLVALMVKLYDYSEFMHPKLVMCIYSWHIYFLLEIILA 186
            +    E P      L Y IK   V L++K Y+YS  +  K+V+ +Y+ HIYF LEIILA
Sbjct: 123 SHEGSTEGP------LIYTIKAVFVVLIIKAYEYSTKLPEKVVLTLYAIHIYFALEIILA 182

Query: 187 AAKFLARNLLGAELEPQFNDPYFSTSLQDFWGRRWNLMVTGILNPTVYKPTIRLAAPVVG 246
           A     R +   ELEPQFN PY +TSLQDFWGRRWNLMVTGIL PTVY+P+++L + V+G
Sbjct: 183 ATAAAVRAMSDLELEPQFNKPYLATSLQDFWGRRWNLMVTGILRPTVYEPSLQLFS-VLG 242

Query: 247 RKWAPLLAVLATFFVSAMMHELIFYYLGRMRPDWEVSRFFAVHGVALTLEILAKKAWPEK 306
             ++ +LA   TF VS +MHELIF+Y+GR+RPDW++  FF ++G   T+EI  KK    +
Sbjct: 243 PNYSQILAAFGTFVVSGIMHELIFFYMGRLRPDWKMMWFFLINGFCTTVEIAIKKTINGR 302

Query: 307 CRRVPAVVSVPLTVGFVFVTGIWMFFPQFVRCGIDVKAFAEYAAVSDF 353
             R P  +S  LT+ FV VT +W+F P+F RC I  KA  EYAA+  F
Sbjct: 303 W-RFPKAISQVLTLTFVMVTALWLFLPEFNRCNIVEKALDEYAAIGAF 332

[Close]

BLAST of MS016073 vs. TAIR 10
Match: AT5G55350.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 271.2 bits (692), Expect = 1.2e-72
Identity = 152/358 (42.46%), Postives = 206/358 (57.54%), Query Frame = 0


Query: 1   MENEIINFINVWLTVCASLCYCYLIGKNVRPGGRRLISIFPVVCLFLYLPLRLSSAHLGG 60
           ME E+++ I VW+    S+ YCY     ++ G  RL+S+ PV  LFL LPL +SS H  G
Sbjct: 1   MEEELMSLIKVWVYAIISISYCYYTSTRIKSGVFRLLSVLPVCVLFLVLPLFVSSVHFSG 60

Query: 61  PTAFFIAWLANFKLLLFSFAGGPLSSDPPLPLRRFLAVSCLPIKIQRNHLQIYQNGENPF 120
            TAFF++WLANFKL+LFSF  GPL    P  L RF+  +C PIK+Q+N            
Sbjct: 61  STAFFLSWLANFKLILFSFDQGPL-FPVPSNLSRFVCFTCFPIKLQQN------------ 120

Query: 121 PKSSPNAPQIETPLPSKSFLSYAIKGFLVALMVKLYDYSEFMHPKLVMCIYSWHIYFLLE 180
                  P+ +  +P   F   A+K     +++ +Y+Y + M P +++ +YS HIY   E
Sbjct: 121 -------PKPQNQMPKWGF---AVKLAFFGVLLHMYEYKQHMSPTVLLVLYSLHIYLEYE 180

Query: 181 IILAAAKFLARNLLGAELEPQFNDPYFSTSLQDFWGRRWNLMVTGILNPTVYKPTIRLAA 240
           I+LA  K L    L  +LEP FN+PY STSLQDFWGRRWNLMV  IL P VY P  ++A 
Sbjct: 181 ILLAPLKVLLSISLWCDLEPHFNEPYLSTSLQDFWGRRWNLMVPAILRPAVYLPVRQMAG 240

Query: 241 PVVGRKWAPLLAVLATFFVSAMMHELIFYYLGRMRPDWEVSRFFAVHGVALTLEILAKKA 300
             +    A  L V A+F VS ++HELIF+Y  R  P  EV+ FF +HGV    E  AK+ 
Sbjct: 241 RKMNSDQALFLGVFASFLVSGVVHELIFFYFTRESPTGEVTLFFVLHGVCTAAECAAKRT 300

Query: 301 WPEKCRRVPAVVSVPLTVGFVFVTGIWMFFPQFVRCGIDVKAFAEYAAVSDFFTKRLF 359
              +  +V  +VS  LTVGFV +TG W+FFP   R G+ ++  A+ A +   F K  F
Sbjct: 301 RLVRRWKVSQMVSRLLTVGFVVMTGGWLFFPHLARSGM-IERLADEAFLFIGFVKHKF 334

[Close]

BLAST of MS016073 vs. TAIR 10
Match: AT5G55340.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 269.2 bits (687), Expect = 4.7e-72
Identity = 156/359 (43.45%), Postives = 208/359 (57.94%), Query Frame = 0


Query: 1   MENEIINFINVWLTVCASLCYCYLIGKNVRPGGRRLISIFPVVCLFLYLPLRLSSAHLGG 60
           M+ E+ N I VW++   S+ YCY I   ++ G  R +S+ PV+ LFL LPL  SS HL  
Sbjct: 1   MDEELKNLIKVWVSAIISISYCYYIPPRIKSGAPRFLSVSPVLALFLVLPLFFSSLHLSL 60

Query: 61  PTAFFIAWLANFKLLLFSFAGGPLSSDPPLPLR--RFLAVSCLPIKIQRNHLQIYQNGEN 120
            TAFF+ WLANFKL+LFSF  GPL    P+P    RF   +C PIK+Q+N          
Sbjct: 61  ITAFFLTWLANFKLILFSFDKGPLI---PIPTNFPRFFCFTCFPIKVQQN---------- 120

Query: 121 PFPKSSPNAPQIETPLPSKSFLSYAIKGFLVALMVKLYDYSEFMHPKLVMCIYSWHIYFL 180
             PKS  + P+          L +AIK  + A+++ LY Y + + P +++ +Y  H+Y  
Sbjct: 121 --PKSQNHLPK----------LVFAIKLAIFAVLLHLYSYRQNLSPTILLGLYFVHLYLE 180

Query: 181 LEIILAAAKFLARNLLGAELEPQFNDPYFSTSLQDFWGRRWNLMVTGILNPTVYKPTIRL 240
           +EIIL   K +    LG +LEPQ N PY +TSLQDFWGRRWNLMV  IL P VY P  R+
Sbjct: 181 IEIILTFVKVVVFISLGCDLEPQSNKPYLATSLQDFWGRRWNLMVPAILRPAVYAPMRRV 240

Query: 241 AAPVVGRKWAPLLAVLATFFVSAMMHELIFYYLGRMRPDWEVSRFFAVHGVALTLEILAK 300
           +   +   WA    +LA F VS ++HEL+F+YL R  P  EV+ FF +HGV   +E+  K
Sbjct: 241 SERKMSSGWALFPGILAAFIVSGLVHELLFFYLIREMPTGEVTLFFVLHGVCTAVELAVK 300

Query: 301 KAWPEKCR-RVPAVVSVPLTVGFVFVTGIWMFFPQFVRCGIDVKAFAEYAAVSDFFTKR 357
           K      R R+   VS  LTVGFVFVTG W+F PQ  R G+ ++ F   A +   F KR
Sbjct: 301 KKTTVAQRWRLSPGVSRVLTVGFVFVTGGWLFTPQLKRSGV-MERFTSEAVLLVEFIKR 333

[Close]

BLAST of MS016073 vs. TAIR 10
Match: AT5G55360.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 267.3 bits (682), Expect = 1.8e-71
Identity = 154/362 (42.54%), Postives = 207/362 (57.18%), Query Frame = 0


Query: 1   MENEIINFINVWLTVCASLCYCYLIGKNVRPGGRRLISIFPVVCLFLYLPLRLSSAHLGG 60
           ME E+ NFI +W++   S+ YCY +   ++ G  RL+S+ PV  LFL  PL  S  H  G
Sbjct: 1   MEEELKNFIKLWISAIISISYCYYLSTGIKAGVFRLLSVLPVCALFLVFPLFFSYVHFSG 60

Query: 61  PTAFFIAWLANFKLLLFSFAGGPLSSDPPLP--LRRFLAVSCLPIKIQRNHLQIYQNGEN 120
             AFF++WLANFKL+LFSF  GPLS   PLP  L RF+ ++C PIK              
Sbjct: 61  CMAFFLSWLANFKLILFSFDQGPLS---PLPRTLSRFICITCFPIK-------------- 120

Query: 121 PFPKSSPNAPQIETPLPSKSFLSYAIKGFLVALMVKLYDYSEFMHPKLVMCIYSWHIYFL 180
             P+ +PN    + P+       +AIK  +  +++++Y+Y +++ P L++   S HI+  
Sbjct: 121 --PQQNPNIQNYKIPI-----WLFAIKVVIFVVLLQMYEYKQYLSPALLLVFNSLHIFLE 180

Query: 181 LEIILAAAKFLARNLLGAELEPQFNDPYFSTSLQDFWGRRWNLMVTGILNPTVYKPTIRL 240
           LEI+    K L    LG +LEPQ N+PY +TSLQDFWGRRWNLMV  IL P VY P  R+
Sbjct: 181 LEIVFMLVKALVFITLGCDLEPQSNEPYLATSLQDFWGRRWNLMVPAILRPAVYLPARRM 240

Query: 241 AAPVVGRKWAPLLAVLATFFVSAMMHELIFYYLGRMRPDWEVSRFFAVHGVALTLEILAK 300
           A   V    A  L V A F VS  +HE++F+YL R  P  EV+ FF +HGV    E+  K
Sbjct: 241 ACRKVNSDQAMFLGVFAAFLVSGAVHEMLFFYLTREVPTGEVTWFFLLHGVCTVAEVAVK 300

Query: 301 KA-WPEKCRRVPAVVSVPLTVGFVFVTGIWMFFPQFVRCGIDVKAFAEYAAVSDFFTKRL 360
           K+ +  +  RV   VS  LTVGFV VT  W FFP  +R GI ++  A  A +   F K  
Sbjct: 301 KSTFVRRWWRVSPTVSRLLTVGFVVVTSGWFFFP-LIRSGI-IERLASEALMCIDFVKHK 336

[Close]

BLAST of MS016073 vs. TAIR 10
Match: AT5G55380.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 251.9 bits (642), Expect = 7.7e-67
Identity = 141/351 (40.17%), Postives = 200/351 (56.98%), Query Frame = 0


Query: 1   MENEIINFINVWLTVCASLCYCYLIGKNVRPGGRRLISIFPVVCLFLYLPLRLSSAHLGG 60
           ME +  N I VW++   SL YCY I   +  G  RL+SI PV  LFL LPL LS  H   
Sbjct: 1   MEEKFRNLIEVWISALISLSYCYYISSKLSKGVLRLLSILPVCILFLVLPLFLSCVHFCA 60

Query: 61  PTAFFIAWLANFKLLLFSFAGGPLSSDPPLPLRRFLAVSCLPIKIQRNHLQIYQNGENPF 120
            +  F++WLANFKLLLF+F  GPL   PP  L RF+  +CLPIKI+          ++P 
Sbjct: 61  ISVLFLSWLANFKLLLFAFDEGPLFPLPP-KLSRFICFACLPIKIR----------QDPS 120

Query: 121 PKSSPNAPQIETPLPSKSFLSYAIKGFLVALMVKLYDYSEFMHPKLVMCIYSWHIYFLLE 180
           P + PN      P P   ++  A+K  ++ +++ +Y+Y + +   +V+ +Y  HIY  +E
Sbjct: 121 PNAIPNL----HPKPMPKWV-LAVKILVLGVLLHVYEYRDGLPRFVVLALYCLHIYLEVE 180

Query: 181 IILAAAKFLARNLLGAELEPQFNDPYFSTSLQDFWGRRWNLMVTGILNPTVYKPTIRLAA 240
           ++L     +   LLG  +EP FN+PY +TSLQDFW RRWNLMV+ +L  TV+ P  R   
Sbjct: 181 LVLVFVGAVVSTLLGCNIEPVFNEPYLATSLQDFWSRRWNLMVSAVLRSTVHIPVQRFFK 240

Query: 241 PVVGRKWAPLLAVLATFFVSAMMHELIFYYLGRMRPDWEVSRFFAVHGVALTLEILAKKA 300
            ++    A    V+A+FFVS +MHEL+++Y+ R  P WEV+ FF +HG A   EI  K+ 
Sbjct: 241 RILSPDGAMFAGVMASFFVSGLMHELLYFYMIRKPPTWEVTCFFVLHGAATATEIAVKRT 300

Query: 301 WPEKCRRVPAVVSVPLTVGFVFVTGIWMFFPQFVRCGIDVKAFAEYAAVSD 352
             +  R     VS  + + FV VTG+W+F  Q +R  +  KA  E   V D
Sbjct: 301 --QWLRPPHRAVSGLVVLTFVSVTGVWLFLAQVLRNNVHEKAIGECLLVLD 333

The following BLAST results are available for this feature:

BLAST of MS016073 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022152669.1	4.4e-205	98.61	acyl-CoA--sterol O-acyltransferase 1-like [Momordica charantia]	[more]
XP_023523550.1	3.4e-133	65.65	LOW QUALITY PROTEIN: acyl-CoA--sterol O-acyltransferase 1-like [Cucurbita pepo s...	[more]
XP_022998466.1	6.4e-132	65.91	acyl-CoA--sterol O-acyltransferase 1-like [Cucurbita maxima]	[more]
XP_022948970.1	1.4e-131	66.19	acyl-CoA--sterol O-acyltransferase 1-like [Cucurbita moschata] >KAG6606895.1 Acy...	[more]
XP_022934183.1	7.8e-130	65.64	acyl-CoA--sterol O-acyltransferase 1-like [Cucurbita moschata]	[more]

BLAST of MS016073 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SV07	6.8e-84	50.57	Acyl-CoA--sterol O-acyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=ASAT1 PE...	[more]
Q9XGY6	2.2e-74	43.02	Long-chain-alcohol O-fatty-acyltransferase OS=Simmondsia chinensis OX=3999 PE=1 ...	[more]
Q9FJ75	1.7e-71	42.46	Probable long-chain-alcohol O-fatty-acyltransferase 4 OS=Arabidopsis thaliana OX...	[more]
Q9FJ76	6.6e-71	43.45	Probable long-chain-alcohol O-fatty-acyltransferase 5 OS=Arabidopsis thaliana OX...	[more]
Q9FJ74	2.5e-70	42.54	Probable long-chain-alcohol O-fatty-acyltransferase 3 OS=Arabidopsis thaliana OX...	[more]

BLAST of MS016073 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DGQ4	2.1e-205	98.61	acyl-CoA--sterol O-acyltransferase 1-like OS=Momordica charantia OX=3673 GN=LOC1...	[more]
A0A6J1KAA2	3.1e-132	65.91	acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita maxima OX=3661 GN=LOC1114...	[more]
A0A6J1GBE5	6.9e-132	66.19	acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita moschata OX=3662 GN=LOC11...	[more]
A0A6J1F6Y6	3.8e-130	65.64	acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita moschata OX=3662 GN=LOC11...	[more]
A0A6J1J5C4	5.0e-130	65.92	acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita maxima OX=3661 GN=LOC1114...	[more]

BLAST of MS016073 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G51970.1	4.8e-85	50.57	acyl-CoA sterol acyl transferase 1	[more]
AT5G55350.1	1.2e-72	42.46	MBOAT (membrane bound O-acyl transferase) family protein	[more]
AT5G55340.1	4.7e-72	43.45	MBOAT (membrane bound O-acyl transferase) family protein	[more]
AT5G55360.1	1.8e-71	42.54	MBOAT (membrane bound O-acyl transferase) family protein	[more]
AT5G55380.1	7.7e-67	40.17	MBOAT (membrane bound O-acyl transferase) family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR017088	Wax synthase, Magnoliopsida	PIRSF	PIRSF037006	Wax_synthase	coord: 1..360 e-value: 1.2E-121 score: 404.0
IPR032805	Wax synthase domain	PFAM	PF13813	MBOAT_2	coord: 199..285 e-value: 6.1E-16 score: 58.5
None	No IPR available	PANTHER	PTHR31595:SF56	ACYL-COA--STEROL O-ACYLTRANSFERASE 1-LIKE	coord: 2..353
IPR044851	Wax synthase	PANTHER	PTHR31595	LONG-CHAIN-ALCOHOL O-FATTY-ACYLTRANSFERASE 3-RELATED	coord: 2..353

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS016073.1	MS016073.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006629	lipid metabolic process
molecular_function	GO:0008374	O-acyltransferase activity