MS016072 (gene) Bitter gourd (TR) v1

Overview


Name	MS016072
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	acyl-CoA--sterol O-acyltransferase 1-like
Location	scaffold9_2: 173592 .. 174996 (-)
RNA-Seq Expression	MS016072
Synteny	MS016072

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGGATGAGCTTCTGAACTTCTTCAAAGTCTGGTTTTCAGTATGGATTTCTCTCTGCTATTGCTATGGCGTCGGAAAAATAATCCGACCGGGAATCGGTAGACTCATCTTCTTCTTCCCGGTGATCTCCCTCTTCCTCTACCTCCCCCTTAATCTCCACTCTCTCCATCTCGGCGGCCTCACCGCCTTCTTCGTTGCTTGGCTCGCCAACTTCAAACTCCTCCTCTTTGCCGTCGACCAGGGCCCTCTCGCCGACCCCAGGATCTCCATAGGTACGTCGTATGAAATGAAAATGATAATAAGTTTCTTATTCACGAATATAATTAAAAAAAAAAAAGTAAAATCAATGGTTCTACTGACATGTTAAATTGCTGCCGAATTCCAATTTAAAAAAAATGATAGTTCTACTGTGATATCATGTGTTTGTATATTACAGAATTGATAGTTCTATCGTGATATCATGTTGAATTGCTACCAAACCCGAATTTTATATACTAGGAATCAATAGTTCTACTATGAGATGATGTTTAAATTGCTCCCAAATCCGAGAATTTGCTAACTTAAATTTAATCGTTTGTTGGACAGGGATTTTCTGTGCCTTGGCTTCTCTCCCCATCAAATTCAACCAAAACCCACTTGAAAAAGGCTCGGAAAATTGCAATGGAGGTGCTCCAATGGAGGCAATTATAAAATCCCATCACCACCACGAGTCGCTATTAAACTACGCCGTGAAGATCTTGCTCGTGGCGGCGCTGGTCCGGGCCTACGATTTCAGCGATCTCCTCCCCCAGAAGCTCCTCTGGCTCCTCCTCTGCTTCCACATCTACTTCCTCCTCGAGCTCTCCCTCGCCGCCGCCGCCGCCTCCGTCCGCTCCTCCCTCGGCGGCGTCGTCGAGCTCCACCCCATGTTCCACCGCCCCTACCTCACCACCTCGCTCCAGGACTTCTGGGGAAGGCGCTGGAACCTCGCCGTCACCGGAATCCTCCGCCCCGCCGTCTACCGCCCCGCCCTACGCGCAGCCGCCAAATTCCTCGGACCCAAATGGGCCCCACTCGCCGCCGTGGCGGCCACGTTTCTGGCGTCGGCGGCGATGCACGAGCTCATGTTCTTCTACATGTCCCGCGCGTGGCCGCCGTCGTGGGACATCTCGGCGTTTTTCGTTCTTCACGGCGGTTGTCTGGCGGCGGAGATGGTGGTGAAGAGGCTGTGGATCGAGAAGTGGCGGCTGCCGCCGCTTCCTCCGGCGGTGTCCGTTCCTCTGACGGTCGGATTCTTCTTCTCGACTTGCTTTTGGCTGTTCTTTCCGCCGTTCATCAACGACTGTAGGGCCGATGTTCGGGTGGTTGAAGAGTACGCGCTCTTGGGCGCGTTTGTAAAGAACAATATTCCTAGTCGTTTCTAT

mRNA sequence

ATGGAGGATGAGCTTCTGAACTTCTTCAAAGTCTGGTTTTCAGTATGGATTTCTCTCTGCTATTGCTATGGCGTCGGAAAAATAATCCGACCGGGAATCGGTAGACTCATCTTCTTCTTCCCGGTGATCTCCCTCTTCCTCTACCTCCCCCTTAATCTCCACTCTCTCCATCTCGGCGGCCTCACCGCCTTCTTCGTTGCTTGGCTCGCCAACTTCAAACTCCTCCTCTTTGCCGTCGACCAGGGCCCTCTCGCCGACCCCAGGATCTCCATAGGGATTTTCTGTGCCTTGGCTTCTCTCCCCATCAAATTCAACCAAAACCCACTTGAAAAAGGCTCGGAAAATTGCAATGGAGGTGCTCCAATGGAGGCAATTATAAAATCCCATCACCACCACGAGTCGCTATTAAACTACGCCGTGAAGATCTTGCTCGTGGCGGCGCTGGTCCGGGCCTACGATTTCAGCGATCTCCTCCCCCAGAAGCTCCTCTGGCTCCTCCTCTGCTTCCACATCTACTTCCTCCTCGAGCTCTCCCTCGCCGCCGCCGCCGCCTCCGTCCGCTCCTCCCTCGGCGGCGTCGTCGAGCTCCACCCCATGTTCCACCGCCCCTACCTCACCACCTCGCTCCAGGACTTCTGGGGAAGGCGCTGGAACCTCGCCGTCACCGGAATCCTCCGCCCCGCCGTCTACCGCCCCGCCCTACGCGCAGCCGCCAAATTCCTCGGACCCAAATGGGCCCCACTCGCCGCCGTGGCGGCCACGTTTCTGGCGTCGGCGGCGATGCACGAGCTCATGTTCTTCTACATGTCCCGCGCGTGGCCGCCGTCGTGGGACATCTCGGCGTTTTTCGTTCTTCACGGCGGTTGTCTGGCGGCGGAGATGGTGGTGAAGAGGCTGTGGATCGAGAAGTGGCGGCTGCCGCCGCTTCCTCCGGCGGTGTCCGTTCCTCTGACGGTCGGATTCTTCTTCTCGACTTGCTTTTGGCTGTTCTTTCCGCCGTTCATCAACGACTGTAGGGCCGATGTTCGGGTGGTTGAAGAGTACGCGCTCTTGGGCGCGTTTGTAAAGAACAATATTCCTAGTCGTTTCTAT

Coding sequence (CDS)

ATGGAGGATGAGCTTCTGAACTTCTTCAAAGTCTGGTTTTCAGTATGGATTTCTCTCTGCTATTGCTATGGCGTCGGAAAAATAATCCGACCGGGAATCGGTAGACTCATCTTCTTCTTCCCGGTGATCTCCCTCTTCCTCTACCTCCCCCTTAATCTCCACTCTCTCCATCTCGGCGGCCTCACCGCCTTCTTCGTTGCTTGGCTCGCCAACTTCAAACTCCTCCTCTTTGCCGTCGACCAGGGCCCTCTCGCCGACCCCAGGATCTCCATAGGGATTTTCTGTGCCTTGGCTTCTCTCCCCATCAAATTCAACCAAAACCCACTTGAAAAAGGCTCGGAAAATTGCAATGGAGGTGCTCCAATGGAGGCAATTATAAAATCCCATCACCACCACGAGTCGCTATTAAACTACGCCGTGAAGATCTTGCTCGTGGCGGCGCTGGTCCGGGCCTACGATTTCAGCGATCTCCTCCCCCAGAAGCTCCTCTGGCTCCTCCTCTGCTTCCACATCTACTTCCTCCTCGAGCTCTCCCTCGCCGCCGCCGCCGCCTCCGTCCGCTCCTCCCTCGGCGGCGTCGTCGAGCTCCACCCCATGTTCCACCGCCCCTACCTCACCACCTCGCTCCAGGACTTCTGGGGAAGGCGCTGGAACCTCGCCGTCACCGGAATCCTCCGCCCCGCCGTCTACCGCCCCGCCCTACGCGCAGCCGCCAAATTCCTCGGACCCAAATGGGCCCCACTCGCCGCCGTGGCGGCCACGTTTCTGGCGTCGGCGGCGATGCACGAGCTCATGTTCTTCTACATGTCCCGCGCGTGGCCGCCGTCGTGGGACATCTCGGCGTTTTTCGTTCTTCACGGCGGTTGTCTGGCGGCGGAGATGGTGGTGAAGAGGCTGTGGATCGAGAAGTGGCGGCTGCCGCCGCTTCCTCCGGCGGTGTCCGTTCCTCTGACGGTCGGATTCTTCTTCTCGACTTGCTTTTGGCTGTTCTTTCCGCCGTTCATCAACGACTGTAGGGCCGATGTTCGGGTGGTTGAAGAGTACGCGCTCTTGGGCGCGTTTGTAAAGAACAATATTCCTAGTCGTTTCTAT

Protein sequence

MEDELLNFFKVWFSVWISLCYCYGVGKIIRPGIGRLIFFFPVISLFLYLPLNLHSLHLGGLTAFFVAWLANFKLLLFAVDQGPLADPRISIGIFCALASLPIKFNQNPLEKGSENCNGGAPMEAIIKSHHHHESLLNYAVKILLVAALVRAYDFSDLLPQKLLWLLLCFHIYFLLELSLAAAAASVRSSLGGVVELHPMFHRPYLTTSLQDFWGRRWNLAVTGILRPAVYRPALRAAAKFLGPKWAPLAAVAATFLASAAMHELMFFYMSRAWPPSWDISAFFVLHGGCLAAEMVVKRLWIEKWRLPPLPPAVSVPLTVGFFFSTCFWLFFPPFINDCRADVRVVEEYALLGAFVKNNIPSRFY

Homology

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BLAST of MS016072 vs. NCBI nr
Match: XP_038905301.1 (acyl-CoA--sterol O-acyltransferase 1-like [Benincasa hispida])

HSP 1 Score: 539.7 bits (1389), Expect = 2.0e-149
Identity = 266/368 (72.28%), Postives = 301/368 (81.79%), Query Frame = 0


Query: 1   MEDELLNFFKVWFSVWISLCYCYGVGKIIRPGIGRLIFFFPVISLFLYLPLNLHSLHLGG 60
           ME+E+ NF KVW S+WI L YCYGVG+ I  GI R +F FPVISLFLYLPL L SLHLGG
Sbjct: 1   MEEEIQNFLKVWISIWICLSYCYGVGERIPHGITRFLFIFPVISLFLYLPLILRSLHLGG 60

Query: 61  LTAFFVAWLANFKLLLFAVDQGPLADPRISIGIFCALASLPIKFNQNPLEKGSENC-NGG 120
           LTAFF+AWLANFKLLLFA  QGPLA P +SIG FCA+AS P+K  QNP +K SE   NGG
Sbjct: 61  LTAFFIAWLANFKLLLFAAGQGPLAAPGMSIGTFCAVASFPVKLKQNPFQKTSEAAINGG 120

Query: 121 APMEAIIKSHH--HHESLLNYAVKILLVAALVRAYDFSDLLPQKLLWLLLCFHIYFLLEL 180
           +       S+H  H +SLLNY VKILLVAALV+AYD++ +LPQKLLWL LCFHIYFLLEL
Sbjct: 121 SARINDTNSNHNRHRQSLLNYTVKILLVAALVKAYDYTHILPQKLLWLFLCFHIYFLLEL 180

Query: 181 SLAAAAASVRSSLGGVVELHPMFHRPYLTTSLQDFWGRRWNLAVTGILRPAVYRPALRAA 240
           SLAAAA++V  + GG VEL P+FH+PYL+TSLQDFWGRRWNL VTGILRPAV+RPA  AA
Sbjct: 181 SLAAAASAV--AFGGTVELLPLFHQPYLSTSLQDFWGRRWNLVVTGILRPAVFRPAASAA 240

Query: 241 AKFLGPKWAPLAAVAATFLASAAMHELMFFYMSRAW-PPSWDISAFFVLHGGCLAAEMVV 300
            K LG +WAPLA V ATFL SA MHELMFFYMSR W PP+W++ AFFV HGGCL AEM V
Sbjct: 241 TKLLGSRWAPLAGVTATFLVSAVMHELMFFYMSRQWPPPTWEVMAFFVFHGGCLVAEMAV 300

Query: 301 KRLWIEKWRLPPLPPAVSVPLTVGFFFSTCFWLFFPPFINDCRADVRVVEEYALLGAFVK 360
           KRLWI+ W++ PLP AVSVP+TVGFFF TCFWLFFPPFIN C+ADVRVVEEYA+ GAFVK
Sbjct: 301 KRLWIDTWQMRPLPAAVSVPVTVGFFFWTCFWLFFPPFINTCKADVRVVEEYAIFGAFVK 360

Query: 361 NNIPSRFY 365
           NNIPS F+
Sbjct: 361 NNIPSSFW 366

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BLAST of MS016072 vs. NCBI nr
Match: XP_023523549.1 (acyl-CoA--sterol O-acyltransferase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 523.1 bits (1346), Expect = 1.9e-144
Identity = 259/368 (70.38%), Postives = 297/368 (80.71%), Query Frame = 0


Query: 1   MEDELLNFFKVWFSVWISLCYCYGVGKIIRPGIGRLIFFFPVISLFLYLPLNLHSLHLGG 60
           MEDE+    KVW S+WI L YCYGVG  I  G  R +F FPVI +FLYLPLNLHSLHLGG
Sbjct: 1   MEDEIGKLLKVWISIWICLGYCYGVGNKIGEGFTRFLFIFPVICIFLYLPLNLHSLHLGG 60

Query: 61  LTAFFVAWLANFKLLLFAVDQGPLADPRISIGIFCALASLPIKFNQNPLEKGSENC-NGG 120
           LTAFFVAWLANFKLLLFA  QGPLA   +SI  FCA+AS PIK  Q P +K SE   NGG
Sbjct: 61  LTAFFVAWLANFKLLLFAAGQGPLATTGMSIAEFCAVASFPIKIKQIPPQKSSEPAGNGG 120

Query: 121 APMEAIIKSH-HHHESLLNYAVKILLVAALVRAYDFSDLLPQKLLWLLLCFHIYFLLELS 180
           A      KS+ +H ES  NYAVKILLVAALV+AYD++ +LPQKL+W LLCFHIYF+L+LS
Sbjct: 121 AATVIDPKSNQNHRESPFNYAVKILLVAALVKAYDYTHILPQKLVWFLLCFHIYFILDLS 180

Query: 181 LAAAAASVRSSLGGVVELHPMFHRPYLTTSLQDFWGRRWNLAVTGILRPAVYRPALRAAA 240
            AAA+A+V  S GG +EL P+FH PYL+TSLQDFWGRRWNLAVTGILRP  YRPA+  A 
Sbjct: 181 FAAASAAV--SHGGTIELLPLFHHPYLSTSLQDFWGRRWNLAVTGILRPVAYRPAVSTAT 240

Query: 241 KFLGPKWAPLAAVAATFLASAAMHELMFFYMSRAW-PPSWDISAFFVLHGGCLAAEMVVK 300
           K +GP+WAPLA VAATFL SAAMHELMFFYMSR W PP+W+++AFFVLHGGCLA EMV K
Sbjct: 241 KLIGPRWAPLAGVAATFLVSAAMHELMFFYMSRQWPPPTWEVAAFFVLHGGCLAVEMVAK 300

Query: 301 RLWIEKWRLPPLPPAVSVPLTVGFFFSTCFWLFFPPFINDCRADVRVVEEYALLGAFVKN 360
           RLWI+ W++PPLP AVSVP+TVGFFF TCFWLFFPPFIN C+ADVRVV+EY + GAF+KN
Sbjct: 301 RLWIDAWKMPPLPAAVSVPVTVGFFFWTCFWLFFPPFINTCKADVRVVKEYEIFGAFLKN 360

Query: 361 N-IPSRFY 365
           N IP+ F+
Sbjct: 361 NIIPTTFF 366

[Close]

BLAST of MS016072 vs. NCBI nr
Match: XP_004149731.1 (acyl-CoA--sterol O-acyltransferase 1 [Cucumis sativus] >KGN58562.1 hypothetical protein Csa_002341 [Cucumis sativus])

HSP 1 Score: 513.1 bits (1320), Expect = 2.0e-141
Identity = 253/362 (69.89%), Postives = 293/362 (80.94%), Query Frame = 0


Query: 1   MEDELLNFFKVWFSVWISLCYCYGVGKIIRPGIGRLIFFFPVISLFLYLPLNLHSLHLGG 60
           MEDE+ NF KVW S+WI L YCYGVG+ I  GI R +F FPVISLFLYLPL LHSLHLGG
Sbjct: 1   MEDEIGNFLKVWISIWICLSYCYGVGRRIPHGITRFLFIFPVISLFLYLPLLLHSLHLGG 60

Query: 61  LTAFFVAWLANFKLLLFAVDQGPLADPRISIGIFCALASLPIKFNQNPLEKGSE-NCNGG 120
           LTAFF+AWLANFKLLLF+   GPLA P ISIG FCA+AS P+K  Q PL+K SE   +GG
Sbjct: 61  LTAFFIAWLANFKLLLFSAGLGPLAAPGISIGTFCAIASFPVKLKQTPLQKTSEFAIHGG 120

Query: 121 APMEAIIKSHHHHESLLNYAVKILLVAALVRAYDFSDLLPQKLLWLLLCFHIYFLLELSL 180
            P ++    + H +S L+YAVKI LVA +V+AY+++ +LPQKL WL LCFHIYFLLELSL
Sbjct: 121 LPSKS--DHNRHRQSPLSYAVKIFLVAVMVKAYNYTHILPQKLHWLFLCFHIYFLLELSL 180

Query: 181 AAAAASVRSSLGGVVELHPMFHRPYLTTSLQDFWGRRWNLAVTGILRPAVYRPALRAAAK 240
           AAAA+ V  + GG +EL P+FH+PY +TSLQDFWGRRWNL VTGILRPAV+RPA  AA+K
Sbjct: 181 AAAASIV--ACGGTLELLPLFHQPYFSTSLQDFWGRRWNLVVTGILRPAVFRPAASAASK 240

Query: 241 FLGPKWAPLAAVAATFLASAAMHELMFFYMSRAW-PPSWDISAFFVLHGGCLAAEMVVKR 300
            LG K  PLA V ATFL SA MHELMFFYMSR W PP+W++SAFFV HGGCL AEM VKR
Sbjct: 241 LLGKKCGPLAGVTATFLVSAVMHELMFFYMSRQWPPPTWEVSAFFVFHGGCLVAEMAVKR 300

Query: 301 LWIEKWRLPPLPPAVSVPLTVGFFFSTCFWLFFPPFINDCRADVRVVEEYALLGAFVKNN 360
           +WI++WR+ PLP  VSVP+TVGFFF +CFWLFFPPFIN C+ADVRVVEEYAL GAF+KNN
Sbjct: 301 VWIQRWRMRPLPWIVSVPVTVGFFFWSCFWLFFPPFINSCKADVRVVEEYALFGAFLKNN 358

[Close]

BLAST of MS016072 vs. NCBI nr
Match: XP_008461897.1 (PREDICTED: acyl-CoA--sterol O-acyltransferase 1-like [Cucumis melo])

HSP 1 Score: 510.0 bits (1312), Expect = 1.7e-140
Identity = 248/366 (67.76%), Postives = 290/366 (79.23%), Query Frame = 0


Query: 1   MEDELLNFFKVWFSVWISLCYCYGVGKIIRPGIGRLIFFFPVISLFLYLPLNLHSLHLGG 60
           ME+E+ NF KVW S WI L YCYGVGK I  GI R +F  PV+SLFLYLPL LHSLHLGG
Sbjct: 49  MEEEIGNFLKVWISTWICLSYCYGVGKRIPHGITRFLFILPVVSLFLYLPLLLHSLHLGG 108

Query: 61  LTAFFVAWLANFKLLLFAVDQGPLADPRISIGIFCALASLPIKFNQNPLEKGSE-NCNGG 120
           LTAFF+AWLANFKLLLF+   GPL+ P IS+G FCA+AS P+K  QNPL+K SE   NG 
Sbjct: 109 LTAFFIAWLANFKLLLFSAGHGPLSAPGISLGTFCAIASFPVKLKQNPLQKASEFAINGE 168

Query: 121 APMEAIIKSHHHHESLLNYAVKILLVAALVRAYDFSDLLPQKLLWLLLCFHIYFLLELSL 180
            P ++    + H +S L+YAVKI LVAA+V+AY+++ +LPQKL WL LCFHIYFLLELSL
Sbjct: 169 LPSKS--DHNRHPQSPLSYAVKIFLVAAMVKAYNYTHILPQKLHWLFLCFHIYFLLELSL 228

Query: 181 AAAAASVRSSLGGVVELHPMFHRPYLTTSLQDFWGRRWNLAVTGILRPAVYRPALRAAAK 240
           AAAA+ V  + GG VEL P+FH PY +TSLQDFWGRRWNL VTGILRPAV+ PA  AA+K
Sbjct: 229 AAAASVV--AFGGTVELLPLFHEPYFSTSLQDFWGRRWNLVVTGILRPAVFSPAASAASK 288

Query: 241 FLGPKWAPLAAVAATFLASAAMHELMFFYMSRAW-PPSWDISAFFVLHGGCLAAEMVVKR 300
             G +W P+  V ATF+ SA MHELMFFYMSR W PP+W++SAFFV HGGCL  EMVVKR
Sbjct: 289 LFGSRWGPITGVTATFVVSAVMHELMFFYMSRQWPPPTWEVSAFFVFHGGCLVGEMVVKR 348

Query: 301 LWIEKWRLPPLPPAVSVPLTVGFFFSTCFWLFFPPFINDCRADVRVVEEYALLGAFVKNN 360
           +WI+KW + PLP  V+VP+TVGFFF +CFWLFFPPFIN C+ADVRVVEEYAL GAFVKNN
Sbjct: 349 VWIQKWGMRPLPSIVTVPVTVGFFFWSCFWLFFPPFINSCKADVRVVEEYALFGAFVKNN 408

Query: 361 IPSRFY 365
           IPS  +
Sbjct: 409 IPSSLF 410

[Close]

BLAST of MS016072 vs. NCBI nr
Match: XP_022998457.1 (acyl-CoA--sterol O-acyltransferase 1-like [Cucurbita maxima])

HSP 1 Score: 508.1 bits (1307), Expect = 6.5e-140
Identity = 252/360 (70.00%), Postives = 288/360 (80.00%), Query Frame = 0


Query: 1   MEDELLNFFKVWFSVWISLCYCYGVGKIIRPGIGRLIFFFPVISLFLYLPLNLHSLHLGG 60
           MEDE+ N  KVW S+WI L YCYGVGK I  G  R +  FPVI +FLYLPL LHSLHLGG
Sbjct: 1   MEDEIGNLLKVWISIWICLGYCYGVGKKIGEGFTRFLLIFPVICIFLYLPLKLHSLHLGG 60

Query: 61  LTAFFVAWLANFKLLLFAVDQGPLADPRISIGIFCALASLPIKFNQNPLEKGSENC-NGG 120
           LTAFFVAWLANFKLLLFA  QGPLA   +SI  FCA+AS PIK  Q   +K SE   NGG
Sbjct: 61  LTAFFVAWLANFKLLLFAAGQGPLATTGMSIAEFCAVASFPIKIKQILPQKSSEPAGNGG 120

Query: 121 APMEAIIKSHHHH-ESLLNYAVKILLVAALVRAYDFSDLLPQKLLWLLLCFHIYFLLELS 180
           A      KS+ +H ES  NYAVKILLVAALV+AYD++ +L QKL+W LLCFHIYF+L+LS
Sbjct: 121 AATVIDPKSNQNHCESPFNYAVKILLVAALVKAYDYTHILHQKLVWFLLCFHIYFILDLS 180

Query: 181 LAAAAASVRSSLGGVVELHPMFHRPYLTTSLQDFWGRRWNLAVTGILRPAVYRPALRAAA 240
            AAA+A+V  S GG +EL P+FH PYL+TSLQDFWGRRWNLAVTGILRP  YRPA+  A 
Sbjct: 181 FAAASAAV--SHGGTIELLPLFHHPYLSTSLQDFWGRRWNLAVTGILRPVAYRPAVSTAT 240

Query: 241 KFLGPKWAPLAAVAATFLASAAMHELMFFYMSRAW-PPSWDISAFFVLHGGCLAAEMVVK 300
           K +GP+WAPLA VAATFL SAAMHELMFFYMSR W PP+W+++AFFVLHGGCLA EMV K
Sbjct: 241 KLIGPRWAPLAGVAATFLVSAAMHELMFFYMSRQWPPPTWEVAAFFVLHGGCLAVEMVAK 300

Query: 301 RLWIEKWRLPPLPPAVSVPLTVGFFFSTCFWLFFPPFINDCRADVRVVEEYALLGAFVKN 358
           RLWI+ W++PPLP  VSVP+TVGFFF TCFWLFFPPFIN C+ADVRVV+EY + GAF+KN
Sbjct: 301 RLWIDAWKMPPLPAVVSVPVTVGFFFWTCFWLFFPPFINTCKADVRVVKEYEIFGAFLKN 358

[Close]

BLAST of MS016072 vs. ExPASy Swiss-Prot
Match: Q9SV07 (Acyl-CoA--sterol O-acyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=ASAT1 PE=1 SV=1)

HSP 1 Score: 286.2 bits (731), Expect = 5.2e-76
Identity = 159/355 (44.79%), Postives = 218/355 (61.41%), Query Frame = 0


Query: 5   LLNFFKVWFSVWISLCYCYGVGKIIRPGIGRLIFFFPVISLFLYLPLNLHSLHLGGLTAF 64
           + +F K W  V ISLCY + + K++  GI RLI FFPV  +F  +P  ++SLHL G+TAF
Sbjct: 1   MASFIKAWGLVIISLCYTFFIAKLVPKGIKRLILFFPVFLIFFIVPFLIYSLHLLGITAF 60

Query: 65  FVAWLANFKLLLFAVDQGPLADPR--ISIGIFCALASLPIKFNQNPLEKGSENCNGGAPM 124
           F+AWLANFKLLLFA+ +GPL+     +S+ IF A++ LPIK   +P              
Sbjct: 61  FIAWLANFKLLLFALGRGPLSSNHKPLSLPIFLAVSCLPIKIQLSPKP------------ 120

Query: 125 EAIIKSHHHH---ESLLNYAVKILLVAALVRAYDFSDLLPQKLLWLLLCFHIYFLLELSL 184
               K+H H    E  L Y +K + V  +++AY++S  LP+K++  L   HIYF LE+ L
Sbjct: 121 ---TKTHSHEGSTEGPLIYTIKAVFVVLIIKAYEYSTKLPEKVVLTLYAIHIYFALEIIL 180

Query: 185 AAAAASVRSSLGGVVELHPMFHRPYLTTSLQDFWGRRWNLAVTGILRPAVYRPALRAAAK 244
           AA AA+VR+     +EL P F++PYL TSLQDFWGRRWNL VTGILRP VY P+L+  + 
Sbjct: 181 AATAAAVRAM--SDLELEPQFNKPYLATSLQDFWGRRWNLMVTGILRPTVYEPSLQLFS- 240

Query: 245 FLGPKWAPLAAVAATFLASAAMHELMFFYMSRAWPPSWDISAFFVLHGGCLAAEMVVKRL 304
            LGP ++ + A   TF+ S  MHEL+FFYM R   P W +  FF+++G C   E+ +K+ 
Sbjct: 241 VLGPNYSQILAAFGTFVVSGIMHELIFFYMGRL-RPDWKMMWFFLINGFCTTVEIAIKKT 300

Query: 305 WIEKWRLPPLPPAVSVPLTVGFFFSTCFWLFFPPFINDCRADVRVVEEYALLGAF 355
              +WR    P A+S  LT+ F   T  WLF P F N C    + ++EYA +GAF
Sbjct: 301 INGRWR---FPKAISQVLTLTFVMVTALWLFLPEF-NRCNIVEKALDEYAAIGAF 332

[Close]

BLAST of MS016072 vs. ExPASy Swiss-Prot
Match: Q9XGY6 (Long-chain-alcohol O-fatty-acyltransferase OS=Simmondsia chinensis OX=3999 PE=1 SV=1)

HSP 1 Score: 253.4 bits (646), Expect = 3.8e-66
Identity = 143/360 (39.72%), Postives = 206/360 (57.22%), Query Frame = 0


Query: 1   MEDELLNFFKVWFSVWISLCYCYGVGKII-RPGIGRLIFFFPVISLFLYLPLNLHSLHLG 60
           +E EL  F +VW S   + CYC  V  +    G  RL+   PV+ LF++LPL L S HLG
Sbjct: 3   VEKELKTFSEVWISAIAAACYCRFVPAVAPHGGALRLLLLLPVVLLFIFLPLRLSSFHLG 62

Query: 61  GLTAFFVAWLANFKLLLFAVDQGPLADPRISIGIFCALASLPIKFNQNPLEKGSENCNGG 120
           G TA ++ WLANFKLLLFA   GPL++P +S+  F +   LPIKF  +P     +N    
Sbjct: 63  GPTALYLVWLANFKLLLFAFHLGPLSNPSLSLLHFISTTLLPIKFRDDPSNDHEKNKR-- 122

Query: 121 APMEAIIKSHHHHESLLNYAVKILLVAALVRAYDFSDLLPQKLLWLLLCFHIYFLLELSL 180
                          ++ +  K++  A +++ Y+F   LP  ++ +L CFH Y   E++L
Sbjct: 123 -------TLSFEWRKVVLFVAKLVFFAGILKIYEFRKDLPHFVISVLYCFHFYLGTEITL 182

Query: 181 AAAAASVRSSLGGVVELHPMFHRPYLTTSLQDFWGRRWNLAVTGILRPAVYRPALRAAAK 240
           AA+A   R++LG  ++L+P F+ PYL TSLQDFWGRRWNL V+ IL    Y+P  R  ++
Sbjct: 183 AASAVIARATLG--LDLYPQFNEPYLATSLQDFWGRRWNLMVSDILGLTTYQPVRRVLSR 242

Query: 241 FLGPKWAPLAAVAATFLASAAMHELMFFYMSRAWPPSWDISAFFVLHGGCLAAEMVVKRL 300
           ++  +W    A+   F  S  MHE+ FFY++RA  PSW+++ FFVLHG C A EMVVK+ 
Sbjct: 243 WVRLRWEVAGAMLVAFTVSGLMHEVFFFYLTRA-RPSWEVTGFFVLHGVCTAVEMVVKKA 302

Query: 301 WIEKWRLPPLPPAVSVPLTVGFFFSTCFWLFFPPFINDCRADVRVVEEYALLGAFVKNNI 360
              K R   L   VS  LTVGF   T  WLF P  +     D++ ++EY ++  + +  +
Sbjct: 303 VSGKVR---LRREVSGALTVGFVMVTGGWLFLPQLVRH-GVDLKTIDEYPVMFNYTQKKL 346

[Close]

BLAST of MS016072 vs. ExPASy Swiss-Prot
Match: Q9FJ76 (Probable long-chain-alcohol O-fatty-acyltransferase 5 OS=Arabidopsis thaliana OX=3702 GN=AT5 PE=2 SV=1)

HSP 1 Score: 238.8 bits (608), Expect = 9.6e-62
Identity = 147/358 (41.06%), Postives = 194/358 (54.19%), Query Frame = 0


Query: 1   MEDELLNFFKVWFSVWISLCYCYGVGKIIRPGIGRLIFFFPVISLFLYLPLNLHSLHLGG 60
           M++EL N  KVW S  IS+ YCY +   I+ G  R +   PV++LFL LPL   SLHL  
Sbjct: 1   MDEELKNLIKVWVSAIISISYCYYIPPRIKSGAPRFLSVSPVLALFLVLPLFFSSLHLSL 60

Query: 61  LTAFFVAWLANFKLLLFAVDQGPLADPRISIGIFCALASLPIKFNQNPLEKGSENCNGGA 120
           +TAFF+ WLANFKL+LF+ D+GPL     +   F      PIK  QNP            
Sbjct: 61  ITAFFLTWLANFKLILFSFDKGPLIPIPTNFPRFFCFTCFPIKVQQNP------------ 120

Query: 121 PMEAIIKSHHHHESLLNYAVKILLVAALVRAYDFSDLLPQKLLWLLLCFHIYFLLELSLA 180
                 KS +H   L+ +A+K+ + A L+  Y +   L   +L  L   H+Y  +E+ L 
Sbjct: 121 ------KSQNHLPKLV-FAIKLAIFAVLLHLYSYRQNLSPTILLGLYFVHLYLEIEIILT 180

Query: 181 AAAASVRSSLGGVVELHPMFHRPYLTTSLQDFWGRRWNLAVTGILRPAVYRPALRAAAKF 240
                V  SLG   +L P  ++PYL TSLQDFWGRRWNL V  ILRPAVY P  R + + 
Sbjct: 181 FVKVVVFISLG--CDLEPQSNKPYLATSLQDFWGRRWNLMVPAILRPAVYAPMRRVSERK 240

Query: 241 LGPKWAPLAAVAATFLASAAMHELMFFYMSRAWPPSWDISAFFVLHGGCLAAEMVVKR-- 300
           +   WA    + A F+ S  +HEL+FFY+ R   P+ +++ FFVLHG C A E+ VK+  
Sbjct: 241 MSSGWALFPGILAAFIVSGLVHELLFFYLIREM-PTGEVTLFFVLHGVCTAVELAVKKKT 300

Query: 301 LWIEKWRLPPLPPAVSVPLTVGFFFSTCFWLFFPPFINDCRADVRVVEEYALLGAFVK 357
              ++WR   L P VS  LTVGF F T  WLF P  +       R   E  LL  F+K
Sbjct: 301 TVAQRWR---LSPGVSRVLTVGFVFVTGGWLFTPQ-LKRSGVMERFTSEAVLLVEFIK 332

[Close]

BLAST of MS016072 vs. ExPASy Swiss-Prot
Match: Q9FJ75 (Probable long-chain-alcohol O-fatty-acyltransferase 4 OS=Arabidopsis thaliana OX=3702 GN=AT4 PE=2 SV=1)

HSP 1 Score: 234.2 bits (596), Expect = 2.4e-60
Identity = 144/358 (40.22%), Postives = 193/358 (53.91%), Query Frame = 0


Query: 1   MEDELLNFFKVWFSVWISLCYCYGVGKIIRPGIGRLIFFFPVISLFLYLPLNLHSLHLGG 60
           ME+EL++  KVW    IS+ YCY     I+ G+ RL+   PV  LFL LPL + S+H  G
Sbjct: 1   MEEELMSLIKVWVYAIISISYCYYTSTRIKSGVFRLLSVLPVCVLFLVLPLFVSSVHFSG 60

Query: 61  LTAFFVAWLANFKLLLFAVDQGPLADPRISIGIFCALASLPIKFNQNPLEKGSENCNGGA 120
            TAFF++WLANFKL+LF+ DQGPL     ++  F      PIK  QNP            
Sbjct: 61  STAFFLSWLANFKLILFSFDQGPLFPVPSNLSRFVCFTCFPIKLQQNP-----------K 120

Query: 121 PMEAIIKSHHHHESLLNYAVKILLVAALVRAYDFSDLLPQKLLWLLLCFHIYFLLELSLA 180
           P   + K          +AVK+     L+  Y++   +   +L +L   HIY  LE  + 
Sbjct: 121 PQNQMPK--------WGFAVKLAFFGVLLHMYEYKQHMSPTVLLVLYSLHIY--LEYEIL 180

Query: 181 AAAASVRSSLGGVVELHPMFHRPYLTTSLQDFWGRRWNLAVTGILRPAVYRPALRAAAKF 240
            A   V  S+    +L P F+ PYL+TSLQDFWGRRWNL V  ILRPAVY P  + A + 
Sbjct: 181 LAPLKVLLSISLWCDLEPHFNEPYLSTSLQDFWGRRWNLMVPAILRPAVYLPVRQMAGRK 240

Query: 241 LGPKWAPLAAVAATFLASAAMHELMFFYMSRAWPPSWDISAFFVLHGGCLAAEMVVKRL- 300
           +    A    V A+FL S  +HEL+FFY +R   P+ +++ FFVLHG C AAE   KR  
Sbjct: 241 MNSDQALFLGVFASFLVSGVVHELIFFYFTRE-SPTGEVTLFFVLHGVCTAAECAAKRTR 300

Query: 301 WIEKWRLPPLPPAVSVPLTVGFFFSTCFWLFFPPFINDCRADVRVVEEYALLGAFVKN 358
            + +W++  +   VS  LTVGF   T  WLFFP        + R+ +E  L   FVK+
Sbjct: 301 LVRRWKVSQM---VSRLLTVGFVVMTGGWLFFPHLARSGMIE-RLADEAFLFIGFVKH 332

[Close]

BLAST of MS016072 vs. ExPASy Swiss-Prot
Match: Q9FJ72 (Probable long-chain-alcohol O-fatty-acyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=AT1 PE=2 SV=1)

HSP 1 Score: 232.3 bits (591), Expect = 9.0e-60
Identity = 139/330 (42.12%), Postives = 184/330 (55.76%), Query Frame = 0


Query: 1   MEDELLNFFKVWFSVWISLCYCYGVGKIIRPGIGRLIFFFPVISLFLYLPLNLHSLHLGG 60
           ME++  N  +VW S  ISL YCY +   +  G+ RL+   PV  LFL LPL L  +H   
Sbjct: 1   MEEKFRNLIEVWISALISLSYCYYISSKLSKGVLRLLSILPVCILFLVLPLFLSCVHFCA 60

Query: 61  LTAFFVAWLANFKLLLFAVDQGPLADPRISIGIFCALASLPIKFNQNPLEKGSENCNGGA 120
           ++  F++WLANFKLLLFA D+GPL      +  F   A LPIK  Q+P      N +   
Sbjct: 61  ISVLFLSWLANFKLLLFAFDEGPLFPLPPKLSRFICFACLPIKIRQDPSPNAIPNLH-PK 120

Query: 121 PMEAIIKSHHHHESLLNYAVKILLVAALVRAYDFSDLLPQKLLWLLLCFHIYFLLELSLA 180
           PM   +            AVKIL++  L+  Y++ D LP+ ++  L C HIY  +EL L 
Sbjct: 121 PMPKWV-----------LAVKILVLGVLLHVYEYRDGLPRFVVLALYCLHIYLEVELVLV 180

Query: 181 AAAASVRSSLGGVVELHPMFHRPYLTTSLQDFWGRRWNLAVTGILRPAVYRPALRAAAKF 240
              A V + LG  +E  P+F+ PYL TSLQDFW RRWNL V+ +LR  V+ P  R   + 
Sbjct: 181 FVGAVVSTLLGCNIE--PVFNEPYLATSLQDFWSRRWNLMVSAVLRSTVHIPVQRFFKRI 240

Query: 241 LGPKWAPLAAVAATFLASAAMHELMFFYMSRAWPPSWDISAFFVLHGGCLAAEMVVKRLW 300
           L P  A  A V A+F  S  MHEL++FYM R  PP+W+++ FFVLHG   A E+ VKR  
Sbjct: 241 LSPDGAMFAGVMASFFVSGLMHELLYFYMIRK-PPTWEVTCFFVLHGAATATEIAVKR-- 300

Query: 301 IEKWRLPPLPPAVSVPLTVGFFFSTCFWLF 331
             +W  PP   AVS  + + F   T  WLF
Sbjct: 301 -TQWLRPP-HRAVSGLVVLTFVSVTGVWLF 311

[Close]

BLAST of MS016072 vs. ExPASy TrEMBL
Match: A0A0A0LD00 (MBOAT_2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G684680 PE=3 SV=1)

HSP 1 Score: 513.1 bits (1320), Expect = 9.7e-142
Identity = 253/362 (69.89%), Postives = 293/362 (80.94%), Query Frame = 0


Query: 1   MEDELLNFFKVWFSVWISLCYCYGVGKIIRPGIGRLIFFFPVISLFLYLPLNLHSLHLGG 60
           MEDE+ NF KVW S+WI L YCYGVG+ I  GI R +F FPVISLFLYLPL LHSLHLGG
Sbjct: 1   MEDEIGNFLKVWISIWICLSYCYGVGRRIPHGITRFLFIFPVISLFLYLPLLLHSLHLGG 60

Query: 61  LTAFFVAWLANFKLLLFAVDQGPLADPRISIGIFCALASLPIKFNQNPLEKGSE-NCNGG 120
           LTAFF+AWLANFKLLLF+   GPLA P ISIG FCA+AS P+K  Q PL+K SE   +GG
Sbjct: 61  LTAFFIAWLANFKLLLFSAGLGPLAAPGISIGTFCAIASFPVKLKQTPLQKTSEFAIHGG 120

Query: 121 APMEAIIKSHHHHESLLNYAVKILLVAALVRAYDFSDLLPQKLLWLLLCFHIYFLLELSL 180
            P ++    + H +S L+YAVKI LVA +V+AY+++ +LPQKL WL LCFHIYFLLELSL
Sbjct: 121 LPSKS--DHNRHRQSPLSYAVKIFLVAVMVKAYNYTHILPQKLHWLFLCFHIYFLLELSL 180

Query: 181 AAAAASVRSSLGGVVELHPMFHRPYLTTSLQDFWGRRWNLAVTGILRPAVYRPALRAAAK 240
           AAAA+ V  + GG +EL P+FH+PY +TSLQDFWGRRWNL VTGILRPAV+RPA  AA+K
Sbjct: 181 AAAASIV--ACGGTLELLPLFHQPYFSTSLQDFWGRRWNLVVTGILRPAVFRPAASAASK 240

Query: 241 FLGPKWAPLAAVAATFLASAAMHELMFFYMSRAW-PPSWDISAFFVLHGGCLAAEMVVKR 300
            LG K  PLA V ATFL SA MHELMFFYMSR W PP+W++SAFFV HGGCL AEM VKR
Sbjct: 241 LLGKKCGPLAGVTATFLVSAVMHELMFFYMSRQWPPPTWEVSAFFVFHGGCLVAEMAVKR 300

Query: 301 LWIEKWRLPPLPPAVSVPLTVGFFFSTCFWLFFPPFINDCRADVRVVEEYALLGAFVKNN 360
           +WI++WR+ PLP  VSVP+TVGFFF +CFWLFFPPFIN C+ADVRVVEEYAL GAF+KNN
Sbjct: 301 VWIQRWRMRPLPWIVSVPVTVGFFFWSCFWLFFPPFINSCKADVRVVEEYALFGAFLKNN 358

[Close]

BLAST of MS016072 vs. ExPASy TrEMBL
Match: A0A1S3CFN5 (acyl-CoA--sterol O-acyltransferase 1-like OS=Cucumis melo OX=3656 GN=LOC103500392 PE=3 SV=1)

HSP 1 Score: 510.0 bits (1312), Expect = 8.2e-141
Identity = 248/366 (67.76%), Postives = 290/366 (79.23%), Query Frame = 0


Query: 1   MEDELLNFFKVWFSVWISLCYCYGVGKIIRPGIGRLIFFFPVISLFLYLPLNLHSLHLGG 60
           ME+E+ NF KVW S WI L YCYGVGK I  GI R +F  PV+SLFLYLPL LHSLHLGG
Sbjct: 49  MEEEIGNFLKVWISTWICLSYCYGVGKRIPHGITRFLFILPVVSLFLYLPLLLHSLHLGG 108

Query: 61  LTAFFVAWLANFKLLLFAVDQGPLADPRISIGIFCALASLPIKFNQNPLEKGSE-NCNGG 120
           LTAFF+AWLANFKLLLF+   GPL+ P IS+G FCA+AS P+K  QNPL+K SE   NG 
Sbjct: 109 LTAFFIAWLANFKLLLFSAGHGPLSAPGISLGTFCAIASFPVKLKQNPLQKASEFAINGE 168

Query: 121 APMEAIIKSHHHHESLLNYAVKILLVAALVRAYDFSDLLPQKLLWLLLCFHIYFLLELSL 180
            P ++    + H +S L+YAVKI LVAA+V+AY+++ +LPQKL WL LCFHIYFLLELSL
Sbjct: 169 LPSKS--DHNRHPQSPLSYAVKIFLVAAMVKAYNYTHILPQKLHWLFLCFHIYFLLELSL 228

Query: 181 AAAAASVRSSLGGVVELHPMFHRPYLTTSLQDFWGRRWNLAVTGILRPAVYRPALRAAAK 240
           AAAA+ V  + GG VEL P+FH PY +TSLQDFWGRRWNL VTGILRPAV+ PA  AA+K
Sbjct: 229 AAAASVV--AFGGTVELLPLFHEPYFSTSLQDFWGRRWNLVVTGILRPAVFSPAASAASK 288

Query: 241 FLGPKWAPLAAVAATFLASAAMHELMFFYMSRAW-PPSWDISAFFVLHGGCLAAEMVVKR 300
             G +W P+  V ATF+ SA MHELMFFYMSR W PP+W++SAFFV HGGCL  EMVVKR
Sbjct: 289 LFGSRWGPITGVTATFVVSAVMHELMFFYMSRQWPPPTWEVSAFFVFHGGCLVGEMVVKR 348

Query: 301 LWIEKWRLPPLPPAVSVPLTVGFFFSTCFWLFFPPFINDCRADVRVVEEYALLGAFVKNN 360
           +WI+KW + PLP  V+VP+TVGFFF +CFWLFFPPFIN C+ADVRVVEEYAL GAFVKNN
Sbjct: 349 VWIQKWGMRPLPSIVTVPVTVGFFFWSCFWLFFPPFINSCKADVRVVEEYALFGAFVKNN 408

Query: 361 IPSRFY 365
           IPS  +
Sbjct: 409 IPSSLF 410

[Close]

BLAST of MS016072 vs. ExPASy TrEMBL
Match: A0A6J1K824 (acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111493084 PE=3 SV=1)

HSP 1 Score: 508.1 bits (1307), Expect = 3.1e-140
Identity = 252/360 (70.00%), Postives = 288/360 (80.00%), Query Frame = 0


Query: 1   MEDELLNFFKVWFSVWISLCYCYGVGKIIRPGIGRLIFFFPVISLFLYLPLNLHSLHLGG 60
           MEDE+ N  KVW S+WI L YCYGVGK I  G  R +  FPVI +FLYLPL LHSLHLGG
Sbjct: 1   MEDEIGNLLKVWISIWICLGYCYGVGKKIGEGFTRFLLIFPVICIFLYLPLKLHSLHLGG 60

Query: 61  LTAFFVAWLANFKLLLFAVDQGPLADPRISIGIFCALASLPIKFNQNPLEKGSENC-NGG 120
           LTAFFVAWLANFKLLLFA  QGPLA   +SI  FCA+AS PIK  Q   +K SE   NGG
Sbjct: 61  LTAFFVAWLANFKLLLFAAGQGPLATTGMSIAEFCAVASFPIKIKQILPQKSSEPAGNGG 120

Query: 121 APMEAIIKSHHHH-ESLLNYAVKILLVAALVRAYDFSDLLPQKLLWLLLCFHIYFLLELS 180
           A      KS+ +H ES  NYAVKILLVAALV+AYD++ +L QKL+W LLCFHIYF+L+LS
Sbjct: 121 AATVIDPKSNQNHCESPFNYAVKILLVAALVKAYDYTHILHQKLVWFLLCFHIYFILDLS 180

Query: 181 LAAAAASVRSSLGGVVELHPMFHRPYLTTSLQDFWGRRWNLAVTGILRPAVYRPALRAAA 240
            AAA+A+V  S GG +EL P+FH PYL+TSLQDFWGRRWNLAVTGILRP  YRPA+  A 
Sbjct: 181 FAAASAAV--SHGGTIELLPLFHHPYLSTSLQDFWGRRWNLAVTGILRPVAYRPAVSTAT 240

Query: 241 KFLGPKWAPLAAVAATFLASAAMHELMFFYMSRAW-PPSWDISAFFVLHGGCLAAEMVVK 300
           K +GP+WAPLA VAATFL SAAMHELMFFYMSR W PP+W+++AFFVLHGGCLA EMV K
Sbjct: 241 KLIGPRWAPLAGVAATFLVSAAMHELMFFYMSRQWPPPTWEVAAFFVLHGGCLAVEMVAK 300

Query: 301 RLWIEKWRLPPLPPAVSVPLTVGFFFSTCFWLFFPPFINDCRADVRVVEEYALLGAFVKN 358
           RLWI+ W++PPLP  VSVP+TVGFFF TCFWLFFPPFIN C+ADVRVV+EY + GAF+KN
Sbjct: 301 RLWIDAWKMPPLPAVVSVPVTVGFFFWTCFWLFFPPFINTCKADVRVVKEYEIFGAFLKN 358

[Close]

BLAST of MS016072 vs. ExPASy TrEMBL
Match: A0A5A7U2E1 (Acyl-CoA--sterol O-acyltransferase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold264G001430 PE=3 SV=1)

HSP 1 Score: 493.0 bits (1268), Expect = 1.0e-135
Identity = 240/356 (67.42%), Postives = 281/356 (78.93%), Query Frame = 0


Query: 1   MEDELLNFFKVWFSVWISLCYCYGVGKIIRPGIGRLIFFFPVISLFLYLPLNLHSLHLGG 60
           ME+E+ NF KVW S WI L YCYGVGK I  GI R +F  PV+SLFLYLPL LHSLHLGG
Sbjct: 1   MEEEIGNFLKVWISTWICLSYCYGVGKRIPHGITRFLFILPVVSLFLYLPLLLHSLHLGG 60

Query: 61  LTAFFVAWLANFKLLLFAVDQGPLADPRISIGIFCALASLPIKFNQNPLEKGSE-NCNGG 120
           LTAFF+AWLANFKLLLF+   GPL+ P IS+G FCA+AS P+K  QNPL+K SE   NG 
Sbjct: 61  LTAFFIAWLANFKLLLFSAGHGPLSAPGISLGTFCAIASFPVKLKQNPLQKASEFAINGE 120

Query: 121 APMEAIIKSHHHHESLLNYAVKILLVAALVRAYDFSDLLPQKLLWLLLCFHIYFLLELSL 180
            P ++    + H +S L+YAVKI LVAA+V+AY+++ +LPQKL WL LCFHIYFLLELSL
Sbjct: 121 LPSKS--DHNRHPQSPLSYAVKIFLVAAMVKAYNYTHILPQKLHWLFLCFHIYFLLELSL 180

Query: 181 AAAAASVRSSLGGVVELHPMFHRPYLTTSLQDFWGRRWNLAVTGILRPAVYRPALRAAAK 240
           AAAA+ V  + GG VEL P+FH PY +TSLQDFWGRRWNL VTGILRPAV+ PA  AA+K
Sbjct: 181 AAAASVV--AFGGTVELLPLFHEPYFSTSLQDFWGRRWNLVVTGILRPAVFSPAASAASK 240

Query: 241 FLGPKWAPLAAVAATFLASAAMHELMFFYMSRAW-PPSWDISAFFVLHGGCLAAEMVVKR 300
             G +W P+  V ATF+ SA MHELMFFYMSR W PP+W++SAFFV HGGCL  EMVVKR
Sbjct: 241 LFGSRWGPITGVTATFVVSAVMHELMFFYMSRQWPPPTWEVSAFFVFHGGCLVGEMVVKR 300

Query: 301 LWIEKWRLPPLPPAVSVPLTVGFFFSTCFWLFFPPFINDCRADVRVVEEYALLGAF 355
           +WI+KW + PLP  V+VP+TVGFFF +CFWLFFPPFIN C+ADVRVVEEYAL G F
Sbjct: 301 VWIQKWGMRPLPSIVTVPVTVGFFFWSCFWLFFPPFINSCKADVRVVEEYALFGLF 352

[Close]

BLAST of MS016072 vs. ExPASy TrEMBL
Match: A0A6J1DI64 (acyl-CoA--sterol O-acyltransferase 1-like OS=Momordica charantia OX=3673 GN=LOC111020278 PE=3 SV=1)

HSP 1 Score: 392.5 bits (1007), Expect = 1.9e-105
Identity = 255/563 (45.29%), Postives = 281/563 (49.91%), Query Frame = 0


Query: 1   MEDELLNFFKVWFSVWISLCYCYGVGKIIRPGIGRLIFFFPVISLFLYLPLNLHSLHLGG 60
           MEDELLNFFKVWFSVWISLCYCYGVGKIIRPG GRLIFFFPVISLFLYLP+NLHSLHLGG
Sbjct: 1   MEDELLNFFKVWFSVWISLCYCYGVGKIIRPGTGRLIFFFPVISLFLYLPINLHSLHLGG 60

Query: 61  LTAFFVAWLANFKLLLFAVDQGPLADPRISIGIFCALASLPIKFNQNPLEKGSENCNGGA 120
           LTAFFVAWLANFKLLLFAVDQGPLADPRISIGIFCALASLPIKFNQNPLEKGSENCNGGA
Sbjct: 61  LTAFFVAWLANFKLLLFAVDQGPLADPRISIGIFCALASLPIKFNQNPLEKGSENCNGGA 120

Query: 121 PMEAIIKSHHHHESLLNYAV---------------------------------------- 180
           PMEAIIKSHHHHESLLNYAV                                        
Sbjct: 121 PMEAIIKSHHHHESLLNYAVHWDSLPVIVPQLPNHISNPLHGKEEIMSITSLNMMADGLA 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 KVSIFVNQFSIYLLHICVCVEEKEEEDEKKSKFQITPLMATEIGIFVQTVLLVCSSLCFC 240

Query: 241 --------------------------------------------------KILL------ 300
                                                             K+LL      
Sbjct: 241 FSIRNSIPHGFLRFLLILPFFFLFLCIPLQFHTIHLQGAIGFFIGWLGSFKLLLFAFGKG 300

Query: 301 ------VAALVRAY------------------------DFSDLLPQKLLWLLL------- 358
                  AA +R +                          S   P KL  LLL       
Sbjct: 301 PLCSAPAAASLRRFLAVGSLPIEIPVHPPQAPAPDSPPVLSLTFPAKLGLLLLTFCAIYF 360

[Close]

BLAST of MS016072 vs. TAIR 10
Match: AT3G51970.1 (acyl-CoA sterol acyl transferase 1 )

HSP 1 Score: 286.2 bits (731), Expect = 3.7e-77
Identity = 159/355 (44.79%), Postives = 218/355 (61.41%), Query Frame = 0


Query: 5   LLNFFKVWFSVWISLCYCYGVGKIIRPGIGRLIFFFPVISLFLYLPLNLHSLHLGGLTAF 64
           + +F K W  V ISLCY + + K++  GI RLI FFPV  +F  +P  ++SLHL G+TAF
Sbjct: 1   MASFIKAWGLVIISLCYTFFIAKLVPKGIKRLILFFPVFLIFFIVPFLIYSLHLLGITAF 60

Query: 65  FVAWLANFKLLLFAVDQGPLADPR--ISIGIFCALASLPIKFNQNPLEKGSENCNGGAPM 124
           F+AWLANFKLLLFA+ +GPL+     +S+ IF A++ LPIK   +P              
Sbjct: 61  FIAWLANFKLLLFALGRGPLSSNHKPLSLPIFLAVSCLPIKIQLSPKP------------ 120

Query: 125 EAIIKSHHHH---ESLLNYAVKILLVAALVRAYDFSDLLPQKLLWLLLCFHIYFLLELSL 184
               K+H H    E  L Y +K + V  +++AY++S  LP+K++  L   HIYF LE+ L
Sbjct: 121 ---TKTHSHEGSTEGPLIYTIKAVFVVLIIKAYEYSTKLPEKVVLTLYAIHIYFALEIIL 180

Query: 185 AAAAASVRSSLGGVVELHPMFHRPYLTTSLQDFWGRRWNLAVTGILRPAVYRPALRAAAK 244
           AA AA+VR+     +EL P F++PYL TSLQDFWGRRWNL VTGILRP VY P+L+  + 
Sbjct: 181 AATAAAVRAM--SDLELEPQFNKPYLATSLQDFWGRRWNLMVTGILRPTVYEPSLQLFS- 240

Query: 245 FLGPKWAPLAAVAATFLASAAMHELMFFYMSRAWPPSWDISAFFVLHGGCLAAEMVVKRL 304
            LGP ++ + A   TF+ S  MHEL+FFYM R   P W +  FF+++G C   E+ +K+ 
Sbjct: 241 VLGPNYSQILAAFGTFVVSGIMHELIFFYMGRL-RPDWKMMWFFLINGFCTTVEIAIKKT 300

Query: 305 WIEKWRLPPLPPAVSVPLTVGFFFSTCFWLFFPPFINDCRADVRVVEEYALLGAF 355
              +WR    P A+S  LT+ F   T  WLF P F N C    + ++EYA +GAF
Sbjct: 301 INGRWR---FPKAISQVLTLTFVMVTALWLFLPEF-NRCNIVEKALDEYAAIGAF 332

[Close]

BLAST of MS016072 vs. TAIR 10
Match: AT5G55340.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 238.8 bits (608), Expect = 6.8e-63
Identity = 147/358 (41.06%), Postives = 194/358 (54.19%), Query Frame = 0


Query: 1   MEDELLNFFKVWFSVWISLCYCYGVGKIIRPGIGRLIFFFPVISLFLYLPLNLHSLHLGG 60
           M++EL N  KVW S  IS+ YCY +   I+ G  R +   PV++LFL LPL   SLHL  
Sbjct: 1   MDEELKNLIKVWVSAIISISYCYYIPPRIKSGAPRFLSVSPVLALFLVLPLFFSSLHLSL 60

Query: 61  LTAFFVAWLANFKLLLFAVDQGPLADPRISIGIFCALASLPIKFNQNPLEKGSENCNGGA 120
           +TAFF+ WLANFKL+LF+ D+GPL     +   F      PIK  QNP            
Sbjct: 61  ITAFFLTWLANFKLILFSFDKGPLIPIPTNFPRFFCFTCFPIKVQQNP------------ 120

Query: 121 PMEAIIKSHHHHESLLNYAVKILLVAALVRAYDFSDLLPQKLLWLLLCFHIYFLLELSLA 180
                 KS +H   L+ +A+K+ + A L+  Y +   L   +L  L   H+Y  +E+ L 
Sbjct: 121 ------KSQNHLPKLV-FAIKLAIFAVLLHLYSYRQNLSPTILLGLYFVHLYLEIEIILT 180

Query: 181 AAAASVRSSLGGVVELHPMFHRPYLTTSLQDFWGRRWNLAVTGILRPAVYRPALRAAAKF 240
                V  SLG   +L P  ++PYL TSLQDFWGRRWNL V  ILRPAVY P  R + + 
Sbjct: 181 FVKVVVFISLG--CDLEPQSNKPYLATSLQDFWGRRWNLMVPAILRPAVYAPMRRVSERK 240

Query: 241 LGPKWAPLAAVAATFLASAAMHELMFFYMSRAWPPSWDISAFFVLHGGCLAAEMVVKR-- 300
           +   WA    + A F+ S  +HEL+FFY+ R   P+ +++ FFVLHG C A E+ VK+  
Sbjct: 241 MSSGWALFPGILAAFIVSGLVHELLFFYLIREM-PTGEVTLFFVLHGVCTAVELAVKKKT 300

Query: 301 LWIEKWRLPPLPPAVSVPLTVGFFFSTCFWLFFPPFINDCRADVRVVEEYALLGAFVK 357
              ++WR   L P VS  LTVGF F T  WLF P  +       R   E  LL  F+K
Sbjct: 301 TVAQRWR---LSPGVSRVLTVGFVFVTGGWLFTPQ-LKRSGVMERFTSEAVLLVEFIK 332

[Close]

BLAST of MS016072 vs. TAIR 10
Match: AT5G55350.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 234.2 bits (596), Expect = 1.7e-61
Identity = 144/358 (40.22%), Postives = 193/358 (53.91%), Query Frame = 0


Query: 1   MEDELLNFFKVWFSVWISLCYCYGVGKIIRPGIGRLIFFFPVISLFLYLPLNLHSLHLGG 60
           ME+EL++  KVW    IS+ YCY     I+ G+ RL+   PV  LFL LPL + S+H  G
Sbjct: 1   MEEELMSLIKVWVYAIISISYCYYTSTRIKSGVFRLLSVLPVCVLFLVLPLFVSSVHFSG 60

Query: 61  LTAFFVAWLANFKLLLFAVDQGPLADPRISIGIFCALASLPIKFNQNPLEKGSENCNGGA 120
            TAFF++WLANFKL+LF+ DQGPL     ++  F      PIK  QNP            
Sbjct: 61  STAFFLSWLANFKLILFSFDQGPLFPVPSNLSRFVCFTCFPIKLQQNP-----------K 120

Query: 121 PMEAIIKSHHHHESLLNYAVKILLVAALVRAYDFSDLLPQKLLWLLLCFHIYFLLELSLA 180
           P   + K          +AVK+     L+  Y++   +   +L +L   HIY  LE  + 
Sbjct: 121 PQNQMPK--------WGFAVKLAFFGVLLHMYEYKQHMSPTVLLVLYSLHIY--LEYEIL 180

Query: 181 AAAASVRSSLGGVVELHPMFHRPYLTTSLQDFWGRRWNLAVTGILRPAVYRPALRAAAKF 240
            A   V  S+    +L P F+ PYL+TSLQDFWGRRWNL V  ILRPAVY P  + A + 
Sbjct: 181 LAPLKVLLSISLWCDLEPHFNEPYLSTSLQDFWGRRWNLMVPAILRPAVYLPVRQMAGRK 240

Query: 241 LGPKWAPLAAVAATFLASAAMHELMFFYMSRAWPPSWDISAFFVLHGGCLAAEMVVKRL- 300
           +    A    V A+FL S  +HEL+FFY +R   P+ +++ FFVLHG C AAE   KR  
Sbjct: 241 MNSDQALFLGVFASFLVSGVVHELIFFYFTRE-SPTGEVTLFFVLHGVCTAAECAAKRTR 300

Query: 301 WIEKWRLPPLPPAVSVPLTVGFFFSTCFWLFFPPFINDCRADVRVVEEYALLGAFVKN 358
            + +W++  +   VS  LTVGF   T  WLFFP        + R+ +E  L   FVK+
Sbjct: 301 LVRRWKVSQM---VSRLLTVGFVVMTGGWLFFPHLARSGMIE-RLADEAFLFIGFVKH 332

[Close]

BLAST of MS016072 vs. TAIR 10
Match: AT5G55380.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 232.3 bits (591), Expect = 6.4e-61
Identity = 139/330 (42.12%), Postives = 184/330 (55.76%), Query Frame = 0


Query: 1   MEDELLNFFKVWFSVWISLCYCYGVGKIIRPGIGRLIFFFPVISLFLYLPLNLHSLHLGG 60
           ME++  N  +VW S  ISL YCY +   +  G+ RL+   PV  LFL LPL L  +H   
Sbjct: 1   MEEKFRNLIEVWISALISLSYCYYISSKLSKGVLRLLSILPVCILFLVLPLFLSCVHFCA 60

Query: 61  LTAFFVAWLANFKLLLFAVDQGPLADPRISIGIFCALASLPIKFNQNPLEKGSENCNGGA 120
           ++  F++WLANFKLLLFA D+GPL      +  F   A LPIK  Q+P      N +   
Sbjct: 61  ISVLFLSWLANFKLLLFAFDEGPLFPLPPKLSRFICFACLPIKIRQDPSPNAIPNLH-PK 120

Query: 121 PMEAIIKSHHHHESLLNYAVKILLVAALVRAYDFSDLLPQKLLWLLLCFHIYFLLELSLA 180
           PM   +            AVKIL++  L+  Y++ D LP+ ++  L C HIY  +EL L 
Sbjct: 121 PMPKWV-----------LAVKILVLGVLLHVYEYRDGLPRFVVLALYCLHIYLEVELVLV 180

Query: 181 AAAASVRSSLGGVVELHPMFHRPYLTTSLQDFWGRRWNLAVTGILRPAVYRPALRAAAKF 240
              A V + LG  +E  P+F+ PYL TSLQDFW RRWNL V+ +LR  V+ P  R   + 
Sbjct: 181 FVGAVVSTLLGCNIE--PVFNEPYLATSLQDFWSRRWNLMVSAVLRSTVHIPVQRFFKRI 240

Query: 241 LGPKWAPLAAVAATFLASAAMHELMFFYMSRAWPPSWDISAFFVLHGGCLAAEMVVKRLW 300
           L P  A  A V A+F  S  MHEL++FYM R  PP+W+++ FFVLHG   A E+ VKR  
Sbjct: 241 LSPDGAMFAGVMASFFVSGLMHELLYFYMIRK-PPTWEVTCFFVLHGAATATEIAVKR-- 300

Query: 301 IEKWRLPPLPPAVSVPLTVGFFFSTCFWLF 331
             +W  PP   AVS  + + F   T  WLF
Sbjct: 301 -TQWLRPP-HRAVSGLVVLTFVSVTGVWLF 311

[Close]

BLAST of MS016072 vs. TAIR 10
Match: AT1G34500.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 229.9 bits (585), Expect = 3.2e-60
Identity = 137/357 (38.38%), Postives = 195/357 (54.62%), Query Frame = 0


Query: 1   MEDELLNFFKVWFSVWISLCYCYGVGKIIRPGIGRLIFFFPVISLFLYLPLNLHSLHLGG 60
           ME+EL NF  VW S  IS+ YCY +   I+ G+ RL    P+  LF  LPL   S+H   
Sbjct: 1   MEEELKNFIIVWISAIISVSYCYYISANIKTGVLRLFSVLPICGLFFVLPLFFSSVHFSS 60

Query: 61  LTAFFVAWLANFKLLLFAVDQGPLADPRISIGIFCALASLPIKFNQNPLEKGSENCNGGA 120
            TAF+++ +A+ KL+LFA DQGPL     ++  F      PIK  +NP  + S+N     
Sbjct: 61  STAFYLSEMASLKLILFAFDQGPLFPVAPNLIQFVCFTCFPIKLQRNPKSQPSQN----- 120

Query: 121 PMEAIIKSHHHHESLLNYAVKILLVAALVRAYDFSDLLPQKLLWLLLCFHIYFLLELSLA 180
                   H H  +   +A+KI++   ++  Y++S  LPQ +L  L   H+Y  LE+ L 
Sbjct: 121 --------HFHKRA---FAIKIMIFGVVLHVYNYSHFLPQTVLLSLCFLHLYVELEILLG 180

Query: 181 AAAASVRSSLGGVVELHPMFHRPYLTTSLQDFWGRRWNLAVTGILRPAVYRPALRAAAKF 240
                +  +LG   +L P F++PYL TSLQDFWGRRWNL V+ +LR  +Y P   A  + 
Sbjct: 181 PLKVLLSMALG--CDLEPQFNKPYLATSLQDFWGRRWNLMVSSVLRSGIYNPVRCACQRP 240

Query: 241 LGPKWAPLAAVAATFLASAAMHELMFFYMSRAWPPSWDISAFFVLHGGCLAAEMVVKR-L 300
           +   WA       TFL S   HEL++FY++R   P+W+++ FFVL+G C   E+ VKR  
Sbjct: 241 MNSGWARFMGYLVTFLVSGLFHELVYFYITRE-TPTWEVTLFFVLNGVCTGTEVAVKRTA 300

Query: 301 WIEKWRLPPLPPAVSVPLTVGFFFSTCFWLFFPPFINDCRADVRVVEEYALLGAFVK 357
           ++++W   P+  +VS  LT+GF   T   LFFP FI     + R  E    L  FVK
Sbjct: 301 FLQRWW--PVRSSVSRLLTMGFVVVTGGLLFFPLFIRSGMMERRANETLFFLD-FVK 335

The following BLAST results are available for this feature:

BLAST of MS016072 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038905301.1	2.0e-149	72.28	acyl-CoA--sterol O-acyltransferase 1-like [Benincasa hispida]	[more]
XP_023523549.1	1.9e-144	70.38	acyl-CoA--sterol O-acyltransferase 1-like [Cucurbita pepo subsp. pepo]	[more]
XP_004149731.1	2.0e-141	69.89	acyl-CoA--sterol O-acyltransferase 1 [Cucumis sativus] >KGN58562.1 hypothetical ...	[more]
XP_008461897.1	1.7e-140	67.76	PREDICTED: acyl-CoA--sterol O-acyltransferase 1-like [Cucumis melo]	[more]
XP_022998457.1	6.5e-140	70.00	acyl-CoA--sterol O-acyltransferase 1-like [Cucurbita maxima]	[more]

BLAST of MS016072 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SV07	5.2e-76	44.79	Acyl-CoA--sterol O-acyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=ASAT1 PE...	[more]
Q9XGY6	3.8e-66	39.72	Long-chain-alcohol O-fatty-acyltransferase OS=Simmondsia chinensis OX=3999 PE=1 ...	[more]
Q9FJ76	9.6e-62	41.06	Probable long-chain-alcohol O-fatty-acyltransferase 5 OS=Arabidopsis thaliana OX...	[more]
Q9FJ75	2.4e-60	40.22	Probable long-chain-alcohol O-fatty-acyltransferase 4 OS=Arabidopsis thaliana OX...	[more]
Q9FJ72	9.0e-60	42.12	Probable long-chain-alcohol O-fatty-acyltransferase 1 OS=Arabidopsis thaliana OX...	[more]

BLAST of MS016072 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LD00	9.7e-142	69.89	MBOAT_2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G684680 PE=...	[more]
A0A1S3CFN5	8.2e-141	67.76	acyl-CoA--sterol O-acyltransferase 1-like OS=Cucumis melo OX=3656 GN=LOC10350039...	[more]
A0A6J1K824	3.1e-140	70.00	acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita maxima OX=3661 GN=LOC1114...	[more]
A0A5A7U2E1	1.0e-135	67.42	Acyl-CoA--sterol O-acyltransferase 1-like OS=Cucumis melo var. makuwa OX=1194695...	[more]
A0A6J1DI64	1.9e-105	45.29	acyl-CoA--sterol O-acyltransferase 1-like OS=Momordica charantia OX=3673 GN=LOC1...	[more]

BLAST of MS016072 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G51970.1	3.7e-77	44.79	acyl-CoA sterol acyl transferase 1	[more]
AT5G55340.1	6.8e-63	41.06	MBOAT (membrane bound O-acyl transferase) family protein	[more]
AT5G55350.1	1.7e-61	40.22	MBOAT (membrane bound O-acyl transferase) family protein	[more]
AT5G55380.1	6.4e-61	42.12	MBOAT (membrane bound O-acyl transferase) family protein	[more]
AT1G34500.1	3.2e-60	38.38	MBOAT (membrane bound O-acyl transferase) family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR017088	Wax synthase, Magnoliopsida	PIRSF	PIRSF037006	Wax_synthase	coord: 1..363 e-value: 9.4E-110 score: 365.0
IPR032805	Wax synthase domain	PFAM	PF13813	MBOAT_2	coord: 197..284 e-value: 2.9E-12 score: 46.7
IPR044851	Wax synthase	PANTHER	PTHR31595	LONG-CHAIN-ALCOHOL O-FATTY-ACYLTRANSFERASE 3-RELATED	coord: 2..358
None	No IPR available	PANTHER	PTHR31595:SF46	ACYL-COA--STEROL O-ACYLTRANSFERASE 1	coord: 2..358

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS016072.1	MS016072.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006629	lipid metabolic process
molecular_function	GO:0008374	O-acyltransferase activity