MS016071 (gene) Bitter gourd (TR) v1

Overview


Name	MS016071
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	acyl-CoA--sterol O-acyltransferase 1-like
Location	scaffold9_2: 167661 .. 168674 (-)
RNA-Seq Expression	MS016071
Synteny	MS016071

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTACCGAAATTGGCATCTTCGTCCAAACCGTGCTTCTGGTTTGCTCCTCCCTCTGTTTCTGCTTCTCCATCAGAAACTCAATCCCCCATGGATTCCTCAGATTTCTCCTCATTCTTCCCTTCTTCTTCCTCTTCCTCTGCATTCCCCTCCAATTTCACACCATCCATCTTCAGGGCGCCATCGGCTTCTTCATCGGCTGGCTCGGCAGCTTCAAGCTCCTCCTCTTCGCCTTCGGCAAAGGCCCTCTCTGCTCCGCCCCTGCCGCCGCCTCCCTCCGCCGCTTCCTCGCCGTCGGCTCCCTCCCAATCGAGATCCCCGTCCACCCCCCGCCGGCACCGGCGCCCGATTCCCCTCCCGTTCTCTCCCTCACCTTCCCGGCCAAATTAGGTCTTCTTCTCCTCACCTTCTGTGCGATCTATTTCCGAAATTACTTCAATCCGTACGCGGTTTTGGTCTTCTATTGCCTTTTGATTTACCTGCTACTGGAAATTCTTGTCGGGGGCGCCGCGGCGCTGGCCCGGACGCTGCTGGGGGTGGATCTGTTGCCGTACTTCGACGAGCCCTACCTCTCTGCTTCGCTGCAGGAATTTTGGGGGCGGAGGTGGAATTTGATGACCACCAGAATCCTCCGCGTCGCCGTCTACGATCCGTGCCGGCGAGTCGCCGGCGCCGCCGTCGGGAAGAGGGCGGCGGTGATGTTGGCGGTGGCTGCGACGTTCGCGGTCTCCGGCCTGATGCACGAACTGATTTACTTCTACATGGGGAGAATGGCGCCGACGTGGGAGGTGACGTGCTTCTTTCTGCTTCACGGGGTGTGTTTGCCGGCGGAGATGGCGGTGAAAGCGGCAGCCGGAGGGAGGTTCCGCCTGCCGCGGGCAGTGTCGGCGCCGTTGACGCTGGGGTTCGTGATGGTGACGAGTTTTTGGCTGTTTTTTCCGCAGTTTCTGAGGTTTAAAGCGGACGTTAGGATGTTTGAGGAGTACGCGGCGTTAGGCGCGTTGGTGAAGAAC

mRNA sequence

ATGGCTACCGAAATTGGCATCTTCGTCCAAACCGTGCTTCTGGTTTGCTCCTCCCTCTGTTTCTGCTTCTCCATCAGAAACTCAATCCCCCATGGATTCCTCAGATTTCTCCTCATTCTTCCCTTCTTCTTCCTCTTCCTCTGCATTCCCCTCCAATTTCACACCATCCATCTTCAGGGCGCCATCGGCTTCTTCATCGGCTGGCTCGGCAGCTTCAAGCTCCTCCTCTTCGCCTTCGGCAAAGGCCCTCTCTGCTCCGCCCCTGCCGCCGCCTCCCTCCGCCGCTTCCTCGCCGTCGGCTCCCTCCCAATCGAGATCCCCGTCCACCCCCCGCCGGCACCGGCGCCCGATTCCCCTCCCGTTCTCTCCCTCACCTTCCCGGCCAAATTAGGTCTTCTTCTCCTCACCTTCTGTGCGATCTATTTCCGAAATTACTTCAATCCGTACGCGGTTTTGGTCTTCTATTGCCTTTTGATTTACCTGCTACTGGAAATTCTTGTCGGGGGCGCCGCGGCGCTGGCCCGGACGCTGCTGGGGGTGGATCTGTTGCCGTACTTCGACGAGCCCTACCTCTCTGCTTCGCTGCAGGAATTTTGGGGGCGGAGGTGGAATTTGATGACCACCAGAATCCTCCGCGTCGCCGTCTACGATCCGTGCCGGCGAGTCGCCGGCGCCGCCGTCGGGAAGAGGGCGGCGGTGATGTTGGCGGTGGCTGCGACGTTCGCGGTCTCCGGCCTGATGCACGAACTGATTTACTTCTACATGGGGAGAATGGCGCCGACGTGGGAGGTGACGTGCTTCTTTCTGCTTCACGGGGTGTGTTTGCCGGCGGAGATGGCGGTGAAAGCGGCAGCCGGAGGGAGGTTCCGCCTGCCGCGGGCAGTGTCGGCGCCGTTGACGCTGGGGTTCGTGATGGTGACGAGTTTTTGGCTGTTTTTTCCGCAGTTTCTGAGGTTTAAAGCGGACGTTAGGATGTTTGAGGAGTACGCGGCGTTAGGCGCGTTGGTGAAGAAC

Coding sequence (CDS)

ATGGCTACCGAAATTGGCATCTTCGTCCAAACCGTGCTTCTGGTTTGCTCCTCCCTCTGTTTCTGCTTCTCCATCAGAAACTCAATCCCCCATGGATTCCTCAGATTTCTCCTCATTCTTCCCTTCTTCTTCCTCTTCCTCTGCATTCCCCTCCAATTTCACACCATCCATCTTCAGGGCGCCATCGGCTTCTTCATCGGCTGGCTCGGCAGCTTCAAGCTCCTCCTCTTCGCCTTCGGCAAAGGCCCTCTCTGCTCCGCCCCTGCCGCCGCCTCCCTCCGCCGCTTCCTCGCCGTCGGCTCCCTCCCAATCGAGATCCCCGTCCACCCCCCGCCGGCACCGGCGCCCGATTCCCCTCCCGTTCTCTCCCTCACCTTCCCGGCCAAATTAGGTCTTCTTCTCCTCACCTTCTGTGCGATCTATTTCCGAAATTACTTCAATCCGTACGCGGTTTTGGTCTTCTATTGCCTTTTGATTTACCTGCTACTGGAAATTCTTGTCGGGGGCGCCGCGGCGCTGGCCCGGACGCTGCTGGGGGTGGATCTGTTGCCGTACTTCGACGAGCCCTACCTCTCTGCTTCGCTGCAGGAATTTTGGGGGCGGAGGTGGAATTTGATGACCACCAGAATCCTCCGCGTCGCCGTCTACGATCCGTGCCGGCGAGTCGCCGGCGCCGCCGTCGGGAAGAGGGCGGCGGTGATGTTGGCGGTGGCTGCGACGTTCGCGGTCTCCGGCCTGATGCACGAACTGATTTACTTCTACATGGGGAGAATGGCGCCGACGTGGGAGGTGACGTGCTTCTTTCTGCTTCACGGGGTGTGTTTGCCGGCGGAGATGGCGGTGAAAGCGGCAGCCGGAGGGAGGTTCCGCCTGCCGCGGGCAGTGTCGGCGCCGTTGACGCTGGGGTTCGTGATGGTGACGAGTTTTTGGCTGTTTTTTCCGCAGTTTCTGAGGTTTAAAGCGGACGTTAGGATGTTTGAGGAGTACGCGGCGTTAGGCGCGTTGGTGAAGAAC

Protein sequence

MATEIGIFVQTVLLVCSSLCFCFSIRNSIPHGFLRFLLILPFFFLFLCIPLQFHTIHLQGAIGFFIGWLGSFKLLLFAFGKGPLCSAPAAASLRRFLAVGSLPIEIPVHPPPAPAPDSPPVLSLTFPAKLGLLLLTFCAIYFRNYFNPYAVLVFYCLLIYLLLEILVGGAAALARTLLGVDLLPYFDEPYLSASLQEFWGRRWNLMTTRILRVAVYDPCRRVAGAAVGKRAAVMLAVAATFAVSGLMHELIYFYMGRMAPTWEVTCFFLLHGVCLPAEMAVKAAAGGRFRLPRAVSAPLTLGFVMVTSFWLFFPQFLRFKADVRMFEEYAALGALVKN

Homology

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BLAST of MS016071 vs. NCBI nr
Match: XP_022152586.1 (acyl-CoA--sterol O-acyltransferase 1-like [Momordica charantia])

HSP 1 Score: 654.1 bits (1686), Expect = 6.8e-184
Identity = 336/338 (99.41%), Postives = 336/338 (99.41%), Query Frame = 0


Query: 1   MATEIGIFVQTVLLVCSSLCFCFSIRNSIPHGFLRFLLILPFFFLFLCIPLQFHTIHLQG 60
           MATEIGIFVQTVLLVCSSLCFCFSIRNSIPHGFLRFLLILPFFFLFLCIPLQFHTIHLQG
Sbjct: 219 MATEIGIFVQTVLLVCSSLCFCFSIRNSIPHGFLRFLLILPFFFLFLCIPLQFHTIHLQG 278

Query: 61  AIGFFIGWLGSFKLLLFAFGKGPLCSAPAAASLRRFLAVGSLPIEIPVHPPPAPAPDSPP 120
           AIGFFIGWLGSFKLLLFAFGKGPLCSAPAAASLRRFLAVGSLPIEIPVHPP APAPDSPP
Sbjct: 279 AIGFFIGWLGSFKLLLFAFGKGPLCSAPAAASLRRFLAVGSLPIEIPVHPPQAPAPDSPP 338

Query: 121 VLSLTFPAKLGLLLLTFCAIYFRNYFNPYAVLVFYCLLIYLLLEILVGGAAALARTLLGV 180
           VLSLTFPAKLGLLLLTFCAIYFRNYFNPYAVLVFYCLLIYLLLEILVGGAAALARTLLGV
Sbjct: 339 VLSLTFPAKLGLLLLTFCAIYFRNYFNPYAVLVFYCLLIYLLLEILVGGAAALARTLLGV 398

Query: 181 DLLPYFDEPYLSASLQEFWGRRWNLMTTRILRVAVYDPCRRVAGAAVGKRAAVMLAVAAT 240
           DL PYFDEPYLSASLQEFWGRRWNLMTTRILRVAVYDPCRRVAGAAVGKRAAVMLAVAAT
Sbjct: 399 DLSPYFDEPYLSASLQEFWGRRWNLMTTRILRVAVYDPCRRVAGAAVGKRAAVMLAVAAT 458

Query: 241 FAVSGLMHELIYFYMGRMAPTWEVTCFFLLHGVCLPAEMAVKAAAGGRFRLPRAVSAPLT 300
           FAVSGLMHELIYFYMGRMAPTWEVTCFFLLHGVCLPAEMAVKAAAGGRFRLPRAVSAPLT
Sbjct: 459 FAVSGLMHELIYFYMGRMAPTWEVTCFFLLHGVCLPAEMAVKAAAGGRFRLPRAVSAPLT 518

Query: 301 LGFVMVTSFWLFFPQFLRFKADVRMFEEYAALGALVKN 339
           LGFVMVTSFWLFFPQFLRFKADVRMFEEYAALGALVKN
Sbjct: 519 LGFVMVTSFWLFFPQFLRFKADVRMFEEYAALGALVKN 556

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BLAST of MS016071 vs. NCBI nr
Match: XP_038905685.1 (acyl-CoA--sterol O-acyltransferase 1-like [Benincasa hispida])

HSP 1 Score: 509.6 bits (1311), Expect = 2.1e-140
Identity = 258/338 (76.33%), Postives = 295/338 (87.28%), Query Frame = 0


Query: 1   MATEIGIFVQTVLLVCSSLCFCFSIRNSIPHGFLRFLLILPFFFLFLCIPLQFHTIHLQG 60
           MA EI IF+QT+ LV SSLCFCF+IRNSIP+GFL+FLLILPFFFLFL IPLQF +IH QG
Sbjct: 1   MAAEIRIFLQTLFLVSSSLCFCFTIRNSIPNGFLKFLLILPFFFLFLYIPLQFQSIHFQG 60

Query: 61  AIGFFIGWLGSFKLLLFAFGKGPLCSAPAAASLRRFLAVGSLPIEIPVHPPPAPAPDSPP 120
           +IGFFIGWLGSFKLLLFAFGKGPLCSA A++SLR FLAVGSLPIEIP H P A    S P
Sbjct: 61  SIGFFIGWLGSFKLLLFAFGKGPLCSAAASSSLRHFLAVGSLPIEIPDHKPAANHSHSNP 120

Query: 121 VLSLTFPAKLGLLLLTFCAIYFRNYFNPYAVLVFYCLLIYLLLEILVGGAAALARTLLGV 180
           VL L+F +KLGLL+LT  AIYF+N F+P A+L+F+C +IYLLLEIL+GGAAALA+  LGV
Sbjct: 121 VLPLSFSSKLGLLILTIVAIYFKNLFHPNAILIFFCFVIYLLLEILLGGAAALAKATLGV 180

Query: 181 DLLPYFDEPYLSASLQEFWGRRWNLMTTRILRVAVYDPCRRVAGAAVGKRAAVMLAVAAT 240
           +LLPYFDEPYLS SLQ+FWGRRWNLMT+RILR+ VYDPCR++    VG++ A M+A+ AT
Sbjct: 181 ELLPYFDEPYLSDSLQDFWGRRWNLMTSRILRLVVYDPCRKITIGIVGRKPAAMMAMTAT 240

Query: 241 FAVSGLMHELIYFYMGRMAPTWEVTCFFLLHGVCLPAEMAVKAAAGGRFRLPRAVSAPLT 300
           FAVSGLMHELIYFYMGR+APTWEVTCFFLLHGVCL AEMA+K+A GGRFRLP  VSA LT
Sbjct: 241 FAVSGLMHELIYFYMGRLAPTWEVTCFFLLHGVCLAAEMAIKSAVGGRFRLPGVVSAALT 300

Query: 301 LGFVMVTSFWLFFPQFLRFKADVRMFEEYAALGALVKN 339
           +GFVM TSFWLFFPQFL+ K+DVRMFEEYAALGA VK+
Sbjct: 301 IGFVMTTSFWLFFPQFLKLKSDVRMFEEYAALGAFVKS 338

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BLAST of MS016071 vs. NCBI nr
Match: XP_022982637.1 (acyl-CoA--sterol O-acyltransferase 1-like [Cucurbita maxima])

HSP 1 Score: 495.4 bits (1274), Expect = 4.0e-136
Identity = 249/338 (73.67%), Postives = 288/338 (85.21%), Query Frame = 0


Query: 1   MATEIGIFVQTVLLVCSSLCFCFSIRNSIPHGFLRFLLILPFFFLFLCIPLQFHTIHLQG 60
           MA EI  F+QT+ L+ SSLC CFSIRNSIP+GFL+F+ +LPFFFLFL IPLQF TIHL+G
Sbjct: 1   MAAEIHAFIQTLFLISSSLCLCFSIRNSIPNGFLKFIFVLPFFFLFLYIPLQFQTIHLRG 60

Query: 61  AIGFFIGWLGSFKLLLFAFGKGPLCSAPAAASLRRFLAVGSLPIEIPVHPPPAPAPDSPP 120
            +GFFIGWLGSFKLLLFAFGKGPLCSA  ++SLRRFLAV SLPI+IP H PP    D  P
Sbjct: 61  TLGFFIGWLGSFKLLLFAFGKGPLCSAATSSSLRRFLAVASLPIDIPEHKPPPNRSDPDP 120

Query: 121 VLSLTFPAKLGLLLLTFCAIYFRNYFNPYAVLVFYCLLIYLLLEILVGGAAALARTLLGV 180
           +L L+F AKLG L+LTF AIYF+N F P  VL+F+CLLIYLLLEIL+GGA A+A  +LGV
Sbjct: 121 LLPLSFAAKLGFLILTFAAIYFKNRFYPNVVLIFFCLLIYLLLEILIGGATAVAEAVLGV 180

Query: 181 DLLPYFDEPYLSASLQEFWGRRWNLMTTRILRVAVYDPCRRVAGAAVGKRAAVMLAVAAT 240
           +LLP+FDEPYLS SLQ+FWGRRWNL+++RILR AV+DPCR++AG  VGK+ AV++AV AT
Sbjct: 181 ELLPFFDEPYLSDSLQDFWGRRWNLVSSRILRPAVFDPCRKLAGTVVGKKPAVIIAVMAT 240

Query: 241 FAVSGLMHELIYFYMGRMAPTWEVTCFFLLHGVCLPAEMAVKAAAGGRFRLPRAVSAPLT 300
           FAVSG+MHELIYFYMGR+APTWEV+ FFL+HGVCL AEMAVK+A G RFR PR VS  LT
Sbjct: 241 FAVSGVMHELIYFYMGRLAPTWEVSWFFLIHGVCLAAEMAVKSAVGRRFRAPRVVSGVLT 300

Query: 301 LGFVMVTSFWLFFPQFLRFKADVRMFEEYAALGALVKN 339
           +GFVM TSFWLFFPQ LRFKADVRM EEYAALGA VKN
Sbjct: 301 MGFVMATSFWLFFPQLLRFKADVRMLEEYAALGAFVKN 338

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BLAST of MS016071 vs. NCBI nr
Match: XP_023528035.1 (acyl-CoA--sterol O-acyltransferase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 487.6 bits (1254), Expect = 8.4e-134
Identity = 245/338 (72.49%), Postives = 285/338 (84.32%), Query Frame = 0


Query: 1   MATEIGIFVQTVLLVCSSLCFCFSIRNSIPHGFLRFLLILPFFFLFLCIPLQFHTIHLQG 60
           MA EI  F+QT+ L+ +SLC CFSIRNSIP+GFL+FL +LPFFFLFL IPLQF TIHL+G
Sbjct: 1   MAAEIHAFIQTLFLISTSLCLCFSIRNSIPNGFLKFLFVLPFFFLFLYIPLQFQTIHLRG 60

Query: 61  AIGFFIGWLGSFKLLLFAFGKGPLCSAPAAASLRRFLAVGSLPIEIPVHPPPAPAPDSPP 120
            +GFFIGWLGSFKLLLFAFGKGPLCSA  ++SLRRFLAV SLPI+IP H PP    D  P
Sbjct: 61  TLGFFIGWLGSFKLLLFAFGKGPLCSAATSSSLRRFLAVASLPIDIPEHKPPPDRSDPDP 120

Query: 121 VLSLTFPAKLGLLLLTFCAIYFRNYFNPYAVLVFYCLLIYLLLEILVGGAAALARTLLGV 180
           +L L+F AKLG L+LTF AIYF+N F P  VL+ +CLLIYLLLEIL+GGA ALA  +LGV
Sbjct: 121 LLPLSFAAKLGFLILTFAAIYFKNRFYPNVVLILFCLLIYLLLEILIGGATALAEAVLGV 180

Query: 181 DLLPYFDEPYLSASLQEFWGRRWNLMTTRILRVAVYDPCRRVAGAAVGKRAAVMLAVAAT 240
           + LP+FDEPYLS SLQ+FWGRRWNL+++RILR AV+DPCR++AG  VGK+ A ++AV AT
Sbjct: 181 EFLPFFDEPYLSDSLQDFWGRRWNLVSSRILRSAVFDPCRKLAGTFVGKKPAAIIAVMAT 240

Query: 241 FAVSGLMHELIYFYMGRMAPTWEVTCFFLLHGVCLPAEMAVKAAAGGRFRLPRAVSAPLT 300
           FAVSG+MHELIYFYMGR+ PTWEV+ FFL+HGVCL AEMAVK+A GGRFR PR VS  LT
Sbjct: 241 FAVSGVMHELIYFYMGRLTPTWEVSWFFLIHGVCLAAEMAVKSAVGGRFRPPRVVSGLLT 300

Query: 301 LGFVMVTSFWLFFPQFLRFKADVRMFEEYAALGALVKN 339
           +GFVM TSFWLFFPQ LRFKADVRM EEYA+LGA VK+
Sbjct: 301 IGFVMATSFWLFFPQLLRFKADVRMLEEYASLGAFVKS 338

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BLAST of MS016071 vs. NCBI nr
Match: XP_022934181.1 (acyl-CoA--sterol O-acyltransferase 1-like [Cucurbita moschata] >KAG6581558.1 Acyl-CoA--sterol O-acyltransferase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 487.6 bits (1254), Expect = 8.4e-134
Identity = 246/338 (72.78%), Postives = 284/338 (84.02%), Query Frame = 0


Query: 1   MATEIGIFVQTVLLVCSSLCFCFSIRNSIPHGFLRFLLILPFFFLFLCIPLQFHTIHLQG 60
           MA EI  F+QT+ L+ SSLC CFSIRNSIP+GFL+FL +LPFFFLFL IPLQF TIHL+G
Sbjct: 1   MAAEIHAFIQTLFLIASSLCLCFSIRNSIPNGFLKFLFVLPFFFLFLYIPLQFQTIHLRG 60

Query: 61  AIGFFIGWLGSFKLLLFAFGKGPLCSAPAAASLRRFLAVGSLPIEIPVHPPPAPAPDSPP 120
            +GFFIGWLGSFKLLLFAFGKGPLC A  ++SLRRFLAV  LPI+IP H  P    D  P
Sbjct: 61  TLGFFIGWLGSFKLLLFAFGKGPLCFAATSSSLRRFLAVACLPIDIPEHKSPPNRSDPDP 120

Query: 121 VLSLTFPAKLGLLLLTFCAIYFRNYFNPYAVLVFYCLLIYLLLEILVGGAAALARTLLGV 180
           +L L+F AKLG L+LTF AIYF+N F P  VL+F+CLLIYLLLEIL+GGA ALA  +LGV
Sbjct: 121 LLPLSFAAKLGFLILTFAAIYFKNRFYPNVVLIFFCLLIYLLLEILIGGATALAEAVLGV 180

Query: 181 DLLPYFDEPYLSASLQEFWGRRWNLMTTRILRVAVYDPCRRVAGAAVGKRAAVMLAVAAT 240
           +LLP+FDEPYLS SLQ+FWGRRWNL+++RILR AV+DPCR++AG  VGK+ A ++AV AT
Sbjct: 181 ELLPFFDEPYLSDSLQDFWGRRWNLVSSRILRPAVFDPCRKLAGTVVGKKPAAIIAVMAT 240

Query: 241 FAVSGLMHELIYFYMGRMAPTWEVTCFFLLHGVCLPAEMAVKAAAGGRFRLPRAVSAPLT 300
           FAVSG+MHELIYFYMGR+ PTWEV+ FFL+HGVCL AEMAVK+A GGRFR PR VS  LT
Sbjct: 241 FAVSGVMHELIYFYMGRLTPTWEVSWFFLIHGVCLAAEMAVKSAVGGRFRPPRVVSGVLT 300

Query: 301 LGFVMVTSFWLFFPQFLRFKADVRMFEEYAALGALVKN 339
           +GFVM TSFWLFFPQ LRFKADVRM EEYAALGA VK+
Sbjct: 301 IGFVMATSFWLFFPQLLRFKADVRMLEEYAALGAFVKS 338

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BLAST of MS016071 vs. ExPASy Swiss-Prot
Match: Q9SV07 (Acyl-CoA--sterol O-acyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=ASAT1 PE=1 SV=1)

HSP 1 Score: 247.7 bits (631), Expect = 1.9e-64
Identity = 142/329 (43.16%), Postives = 196/329 (59.57%), Query Frame = 0


Query: 8   FVQTVLLVCSSLCFCFSIRNSIPHGFLRFLLILPFFFLFLCIPLQFHTIHLQGAIGFFIG 67
           F++   LV  SLC+ F I   +P G  R +L  P F +F  +P   +++HL G   FFI 
Sbjct: 4   FIKAWGLVIISLCYTFFIAKLVPKGIKRLILFFPVFLIFFIVPFLIYSLHLLGITAFFIA 63

Query: 68  WLGSFKLLLFAFGKGPLCSAPAAASLRRFLAVGSLPIEIPVHPPPAP--APDSPPVLSLT 127
           WL +FKLLLFA G+GPL S     SL  FLAV  LPI+I + P P    + +      L 
Sbjct: 64  WLANFKLLLFALGRGPLSSNHKPLSLPIFLAVSCLPIKIQLSPKPTKTHSHEGSTEGPLI 123

Query: 128 FPAKLGLLLLTFCAIYFRNYFNPYAVLVFYCLLIYLLLEILVGGAAALARTLLGVDLLPY 187
           +  K   ++L   A  +        VL  Y + IY  LEI++   AA  R +  ++L P 
Sbjct: 124 YTIKAVFVVLIIKAYEYSTKLPEKVVLTLYAIHIYFALEIILAATAAAVRAMSDLELEPQ 183

Query: 188 FDEPYLSASLQEFWGRRWNLMTTRILRVAVYDPCRRVAGAAVGKRAAVMLAVAATFAVSG 247
           F++PYL+ SLQ+FWGRRWNLM T ILR  VY+P  ++  + +G   + +LA   TF VSG
Sbjct: 184 FNKPYLATSLQDFWGRRWNLMVTGILRPTVYEPSLQLF-SVLGPNYSQILAAFGTFVVSG 243

Query: 248 LMHELIYFYMGRMAPTWEVTCFFLLHGVCLPAEMAVKAAAGGRFRLPRAVSAPLTLGFVM 307
           +MHELI+FYMGR+ P W++  FFL++G C   E+A+K    GR+R P+A+S  LTL FVM
Sbjct: 244 IMHELIFFYMGRLRPDWKMMWFFLINGFCTTVEIAIKKTINGRWRFPKAISQVLTLTFVM 303

Query: 308 VTSFWLFFPQFLRFKADVRMFEEYAALGA 335
           VT+ WLF P+F R     +  +EYAA+GA
Sbjct: 304 VTALWLFLPEFNRCNIVEKALDEYAAIGA 331

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BLAST of MS016071 vs. ExPASy Swiss-Prot
Match: Q9XGY6 (Long-chain-alcohol O-fatty-acyltransferase OS=Simmondsia chinensis OX=3999 PE=1 SV=1)

HSP 1 Score: 226.9 bits (577), Expect = 3.5e-58
Identity = 142/336 (42.26%), Postives = 188/336 (55.95%), Query Frame = 0


Query: 4   EIGIFVQTVLLVCSSLCFCFSIRNSIPH-GFLRFLLILPFFFLFLCIPLQFHTIHLQGAI 63
           E+  F +  +   ++ C+C  +    PH G LR LL+LP   LF+ +PL+  + HL G  
Sbjct: 6   ELKTFSEVWISAIAAACYCRFVPAVAPHGGALRLLLLLPVVLLFIFLPLRLSSFHLGGPT 65

Query: 64  GFFIGWLGSFKLLLFAFGKGPLCSAPAAASLRRFLAVGSLPIEIPVHPPPAPAPDSPPVL 123
             ++ WL +FKLLLFAF  GPL +   + SL  F++   LPI+     P      +   L
Sbjct: 66  ALYLVWLANFKLLLFAFHLGPLSN--PSLSLLHFISTTLLPIKFR-DDPSNDHEKNKRTL 125

Query: 124 S------LTFPAKLGLLLLTFCAIYFRNYFNPYAVLVFYCLLIYLLLEILVGGAAALART 183
           S      + F AKL           FR     + + V YC   YL  EI +  +A +AR 
Sbjct: 126 SFEWRKVVLFVAKLVFFAGILKIYEFRKDLPHFVISVLYCFHFYLGTEITLAASAVIARA 185

Query: 184 LLGVDLLPYFDEPYLSASLQEFWGRRWNLMTTRILRVAVYDPCRRVAGAAVGKRAAVMLA 243
            LG+DL P F+EPYL+ SLQ+FWGRRWNLM + IL +  Y P RRV    V  R  V  A
Sbjct: 186 TLGLDLYPQFNEPYLATSLQDFWGRRWNLMVSDILGLTTYQPVRRVLSRWVRLRWEVAGA 245

Query: 244 VAATFAVSGLMHELIYFYMGRMAPTWEVTCFFLLHGVCLPAEMAVKAAAGGRFRLPRAVS 303
           +   F VSGLMHE+ +FY+ R  P+WEVT FF+LHGVC   EM VK A  G+ RL R VS
Sbjct: 246 MLVAFTVSGLMHEVFFFYLTRARPSWEVTGFFVLHGVCTAVEMVVKKAVSGKVRLRREVS 305

Query: 304 APLTLGFVMVTSFWLFFPQFLRFKADVRMFEEYAAL 333
             LT+GFVMVT  WLF PQ +R   D++  +EY  +
Sbjct: 306 GALTVGFVMVTGGWLFLPQLVRHGVDLKTIDEYPVM 338

[Close]

BLAST of MS016071 vs. ExPASy Swiss-Prot
Match: Q9FJ75 (Probable long-chain-alcohol O-fatty-acyltransferase 4 OS=Arabidopsis thaliana OX=3702 GN=AT4 PE=2 SV=1)

HSP 1 Score: 212.6 bits (540), Expect = 6.8e-54
Identity = 135/339 (39.82%), Postives = 189/339 (55.75%), Query Frame = 0


Query: 1   MATEIGIFVQTVLLVCSSLCFCFSIRNSIPHGFLRFLLILPFFFLFLCIPLQFHTIHLQG 60
           M  E+   ++  +    S+ +C+     I  G  R L +LP   LFL +PL   ++H  G
Sbjct: 1   MEEELMSLIKVWVYAIISISYCYYTSTRIKSGVFRLLSVLPVCVLFLVLPLFVSSVHFSG 60

Query: 61  AIGFFIGWLGSFKLLLFAFGKGPLCSAPAAASLRRFLAVGSLPIEIPVHPPPAPAPDSPP 120
           +  FF+ WL +FKL+LF+F +GPL   P  ++L RF+     PI++  +P P        
Sbjct: 61  STAFFLSWLANFKLILFSFDQGPL--FPVPSNLSRFVCFTCFPIKLQQNPKP-----QNQ 120

Query: 121 VLSLTFPAKLGLLLLTFCAIYFRNYFNPYAVLVFYCLLIYLLLEILVGGAAALARTLLGV 180
           +    F  KL    +      ++ + +P  +LV Y L IYL  EIL+     L    L  
Sbjct: 121 MPKWGFAVKLAFFGVLLHMYEYKQHMSPTVLLVLYSLHIYLEYEILLAPLKVLLSISLWC 180

Query: 181 DLLPYFDEPYLSASLQEFWGRRWNLMTTRILRVAVYDPCRRVAGAAVGKRAAVMLAVAAT 240
           DL P+F+EPYLS SLQ+FWGRRWNLM   ILR AVY P R++AG  +    A+ L V A+
Sbjct: 181 DLEPHFNEPYLSTSLQDFWGRRWNLMVPAILRPAVYLPVRQMAGRKMNSDQALFLGVFAS 240

Query: 241 FAVSGLMHELIYFYMGRMAPTWEVTCFFLLHGVCLPAEMAVK-AAAGGRFRLPRAVSAPL 300
           F VSG++HELI+FY  R +PT EVT FF+LHGVC  AE A K      R+++ + VS  L
Sbjct: 241 FLVSGVVHELIFFYFTRESPTGEVTLFFVLHGVCTAAECAAKRTRLVRRWKVSQMVSRLL 300

Query: 301 TLGFVMVTSFWLFFPQFLRFKADVRMFEEYAALGALVKN 339
           T+GFV++T  WLFFP   R     R+ +E       VK+
Sbjct: 301 TVGFVVMTGGWLFFPHLARSGMIERLADEAFLFIGFVKH 332

[Close]

BLAST of MS016071 vs. ExPASy Swiss-Prot
Match: Q9FJ74 (Probable long-chain-alcohol O-fatty-acyltransferase 3 OS=Arabidopsis thaliana OX=3702 GN=AT3 PE=3 SV=1)

HSP 1 Score: 211.1 bits (536), Expect = 2.0e-53
Identity = 134/316 (42.41%), Postives = 183/316 (57.91%), Query Frame = 0


Query: 1   MATEIGIFVQTVLLVCSSLCFCFSIRNSIPHGFLRFLLILPFFFLFLCIPLQFHTIHLQG 60
           M  E+  F++  +    S+ +C+ +   I  G  R L +LP   LFL  PL F  +H  G
Sbjct: 1   MEEELKNFIKLWISAIISISYCYYLSTGIKAGVFRLLSVLPVCALFLVFPLFFSYVHFSG 60

Query: 61  AIGFFIGWLGSFKLLLFAFGKGPLCSAPAAASLRRFLAVGSLPIEIPVHPPPAPAPDSPP 120
            + FF+ WL +FKL+LF+F +GPL  +P   +L RF+ +   PI+    P   P   +  
Sbjct: 61  CMAFFLSWLANFKLILFSFDQGPL--SPLPRTLSRFICITCFPIK----PQQNPNIQNYK 120

Query: 121 VLSLTFPAKLGLLLLTFCAIYFRNYFNPYAVLVFYCLLIYLLLEILVGGAAALARTLLGV 180
           +    F  K+ + ++      ++ Y +P  +LVF  L I+L LEI+     AL    LG 
Sbjct: 121 IPIWLFAIKVVIFVVLLQMYEYKQYLSPALLLVFNSLHIFLELEIVFMLVKALVFITLGC 180

Query: 181 DLLPYFDEPYLSASLQEFWGRRWNLMTTRILRVAVYDPCRRVAGAAVGKRAAVMLAVAAT 240
           DL P  +EPYL+ SLQ+FWGRRWNLM   ILR AVY P RR+A   V    A+ L V A 
Sbjct: 181 DLEPQSNEPYLATSLQDFWGRRWNLMVPAILRPAVYLPARRMACRKVNSDQAMFLGVFAA 240

Query: 241 FAVSGLMHELIYFYMGRMAPTWEVTCFFLLHGVCLPAEMAVKAAAGGR--FRLPRAVSAP 300
           F VSG +HE+++FY+ R  PT EVT FFLLHGVC  AE+AVK +   R  +R+   VS  
Sbjct: 241 FLVSGAVHEMLFFYLTREVPTGEVTWFFLLHGVCTVAEVAVKKSTFVRRWWRVSPTVSRL 300

Query: 301 LTLGFVMVTSFWLFFP 315
           LT+GFV+VTS W FFP
Sbjct: 301 LTVGFVVVTSGWFFFP 310

[Close]

BLAST of MS016071 vs. ExPASy Swiss-Prot
Match: Q9FJ72 (Probable long-chain-alcohol O-fatty-acyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=AT1 PE=2 SV=1)

HSP 1 Score: 206.1 bits (523), Expect = 6.4e-52
Identity = 132/304 (43.42%), Postives = 172/304 (56.58%), Query Frame = 0


Query: 18  SLCFCFSIRNSIPHGFLRFLLILPFFFLFLCIPLQFHTIHLQGAIGFFIGWLGSFKLLLF 77
           SL +C+ I + +  G LR L ILP   LFL +PL    +H       F+ WL +FKLLLF
Sbjct: 18  SLSYCYYISSKLSKGVLRLLSILPVCILFLVLPLFLSCVHFCAISVLFLSWLANFKLLLF 77

Query: 78  AFGKGPLCSAPAAASLRRFLAVGSLPIEIPVHPPPAPAPD--SPPVLSLTFPAKLGLLLL 137
           AF +GPL   P    L RF+    LPI+I   P P   P+    P+       K+ +L +
Sbjct: 78  AFDEGPL--FPLPPKLSRFICFACLPIKIRQDPSPNAIPNLHPKPMPKWVLAVKILVLGV 137

Query: 138 TFCAIYFRNYFNPYAVLVFYCLLIYLLLEILVGGAAALARTLLGVDLLPYFDEPYLSASL 197
                 +R+    + VL  YCL IYL +E+++    A+  TLLG ++ P F+EPYL+ SL
Sbjct: 138 LLHVYEYRDGLPRFVVLALYCLHIYLEVELVLVFVGAVVSTLLGCNIEPVFNEPYLATSL 197

Query: 198 QEFWGRRWNLMTTRILRVAVYDPCRRVAGAAVGKRAAVMLAVAATFAVSGLMHELIYFYM 257
           Q+FW RRWNLM + +LR  V+ P +R     +    A+   V A+F VSGLMHEL+YFYM
Sbjct: 198 QDFWSRRWNLMVSAVLRSTVHIPVQRFFKRILSPDGAMFAGVMASFFVSGLMHELLYFYM 257

Query: 258 GRMAPTWEVTCFFLLHGVCLPAEMAVKAAAGGRFRLP-RAVSAPLTLGFVMVTSFWLFFP 317
            R  PTWEVTCFF+LHG     E+AVK       R P RAVS  + L FV VT  WLF  
Sbjct: 258 IRKPPTWEVTCFFVLHGAATATEIAVKRTQW--LRPPHRAVSGLVVLTFVSVTGVWLFLA 317

Query: 318 QFLR 319
           Q LR
Sbjct: 318 QVLR 317

[Close]

BLAST of MS016071 vs. ExPASy TrEMBL
Match: A0A6J1DI64 (acyl-CoA--sterol O-acyltransferase 1-like OS=Momordica charantia OX=3673 GN=LOC111020278 PE=3 SV=1)

HSP 1 Score: 654.1 bits (1686), Expect = 3.3e-184
Identity = 336/338 (99.41%), Postives = 336/338 (99.41%), Query Frame = 0


Query: 1   MATEIGIFVQTVLLVCSSLCFCFSIRNSIPHGFLRFLLILPFFFLFLCIPLQFHTIHLQG 60
           MATEIGIFVQTVLLVCSSLCFCFSIRNSIPHGFLRFLLILPFFFLFLCIPLQFHTIHLQG
Sbjct: 219 MATEIGIFVQTVLLVCSSLCFCFSIRNSIPHGFLRFLLILPFFFLFLCIPLQFHTIHLQG 278

Query: 61  AIGFFIGWLGSFKLLLFAFGKGPLCSAPAAASLRRFLAVGSLPIEIPVHPPPAPAPDSPP 120
           AIGFFIGWLGSFKLLLFAFGKGPLCSAPAAASLRRFLAVGSLPIEIPVHPP APAPDSPP
Sbjct: 279 AIGFFIGWLGSFKLLLFAFGKGPLCSAPAAASLRRFLAVGSLPIEIPVHPPQAPAPDSPP 338

Query: 121 VLSLTFPAKLGLLLLTFCAIYFRNYFNPYAVLVFYCLLIYLLLEILVGGAAALARTLLGV 180
           VLSLTFPAKLGLLLLTFCAIYFRNYFNPYAVLVFYCLLIYLLLEILVGGAAALARTLLGV
Sbjct: 339 VLSLTFPAKLGLLLLTFCAIYFRNYFNPYAVLVFYCLLIYLLLEILVGGAAALARTLLGV 398

Query: 181 DLLPYFDEPYLSASLQEFWGRRWNLMTTRILRVAVYDPCRRVAGAAVGKRAAVMLAVAAT 240
           DL PYFDEPYLSASLQEFWGRRWNLMTTRILRVAVYDPCRRVAGAAVGKRAAVMLAVAAT
Sbjct: 399 DLSPYFDEPYLSASLQEFWGRRWNLMTTRILRVAVYDPCRRVAGAAVGKRAAVMLAVAAT 458

Query: 241 FAVSGLMHELIYFYMGRMAPTWEVTCFFLLHGVCLPAEMAVKAAAGGRFRLPRAVSAPLT 300
           FAVSGLMHELIYFYMGRMAPTWEVTCFFLLHGVCLPAEMAVKAAAGGRFRLPRAVSAPLT
Sbjct: 459 FAVSGLMHELIYFYMGRMAPTWEVTCFFLLHGVCLPAEMAVKAAAGGRFRLPRAVSAPLT 518

Query: 301 LGFVMVTSFWLFFPQFLRFKADVRMFEEYAALGALVKN 339
           LGFVMVTSFWLFFPQFLRFKADVRMFEEYAALGALVKN
Sbjct: 519 LGFVMVTSFWLFFPQFLRFKADVRMFEEYAALGALVKN 556

[Close]

BLAST of MS016071 vs. ExPASy TrEMBL
Match: A0A6J1IX47 (acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111481451 PE=3 SV=1)

HSP 1 Score: 495.4 bits (1274), Expect = 1.9e-136
Identity = 249/338 (73.67%), Postives = 288/338 (85.21%), Query Frame = 0


Query: 1   MATEIGIFVQTVLLVCSSLCFCFSIRNSIPHGFLRFLLILPFFFLFLCIPLQFHTIHLQG 60
           MA EI  F+QT+ L+ SSLC CFSIRNSIP+GFL+F+ +LPFFFLFL IPLQF TIHL+G
Sbjct: 1   MAAEIHAFIQTLFLISSSLCLCFSIRNSIPNGFLKFIFVLPFFFLFLYIPLQFQTIHLRG 60

Query: 61  AIGFFIGWLGSFKLLLFAFGKGPLCSAPAAASLRRFLAVGSLPIEIPVHPPPAPAPDSPP 120
            +GFFIGWLGSFKLLLFAFGKGPLCSA  ++SLRRFLAV SLPI+IP H PP    D  P
Sbjct: 61  TLGFFIGWLGSFKLLLFAFGKGPLCSAATSSSLRRFLAVASLPIDIPEHKPPPNRSDPDP 120

Query: 121 VLSLTFPAKLGLLLLTFCAIYFRNYFNPYAVLVFYCLLIYLLLEILVGGAAALARTLLGV 180
           +L L+F AKLG L+LTF AIYF+N F P  VL+F+CLLIYLLLEIL+GGA A+A  +LGV
Sbjct: 121 LLPLSFAAKLGFLILTFAAIYFKNRFYPNVVLIFFCLLIYLLLEILIGGATAVAEAVLGV 180

Query: 181 DLLPYFDEPYLSASLQEFWGRRWNLMTTRILRVAVYDPCRRVAGAAVGKRAAVMLAVAAT 240
           +LLP+FDEPYLS SLQ+FWGRRWNL+++RILR AV+DPCR++AG  VGK+ AV++AV AT
Sbjct: 181 ELLPFFDEPYLSDSLQDFWGRRWNLVSSRILRPAVFDPCRKLAGTVVGKKPAVIIAVMAT 240

Query: 241 FAVSGLMHELIYFYMGRMAPTWEVTCFFLLHGVCLPAEMAVKAAAGGRFRLPRAVSAPLT 300
           FAVSG+MHELIYFYMGR+APTWEV+ FFL+HGVCL AEMAVK+A G RFR PR VS  LT
Sbjct: 241 FAVSGVMHELIYFYMGRLAPTWEVSWFFLIHGVCLAAEMAVKSAVGRRFRAPRVVSGVLT 300

Query: 301 LGFVMVTSFWLFFPQFLRFKADVRMFEEYAALGALVKN 339
           +GFVM TSFWLFFPQ LRFKADVRM EEYAALGA VKN
Sbjct: 301 MGFVMATSFWLFFPQLLRFKADVRMLEEYAALGAFVKN 338

[Close]

BLAST of MS016071 vs. ExPASy TrEMBL
Match: A0A6J1F144 (acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111441426 PE=3 SV=1)

HSP 1 Score: 487.6 bits (1254), Expect = 4.1e-134
Identity = 246/338 (72.78%), Postives = 284/338 (84.02%), Query Frame = 0


Query: 1   MATEIGIFVQTVLLVCSSLCFCFSIRNSIPHGFLRFLLILPFFFLFLCIPLQFHTIHLQG 60
           MA EI  F+QT+ L+ SSLC CFSIRNSIP+GFL+FL +LPFFFLFL IPLQF TIHL+G
Sbjct: 1   MAAEIHAFIQTLFLIASSLCLCFSIRNSIPNGFLKFLFVLPFFFLFLYIPLQFQTIHLRG 60

Query: 61  AIGFFIGWLGSFKLLLFAFGKGPLCSAPAAASLRRFLAVGSLPIEIPVHPPPAPAPDSPP 120
            +GFFIGWLGSFKLLLFAFGKGPLC A  ++SLRRFLAV  LPI+IP H  P    D  P
Sbjct: 61  TLGFFIGWLGSFKLLLFAFGKGPLCFAATSSSLRRFLAVACLPIDIPEHKSPPNRSDPDP 120

Query: 121 VLSLTFPAKLGLLLLTFCAIYFRNYFNPYAVLVFYCLLIYLLLEILVGGAAALARTLLGV 180
           +L L+F AKLG L+LTF AIYF+N F P  VL+F+CLLIYLLLEIL+GGA ALA  +LGV
Sbjct: 121 LLPLSFAAKLGFLILTFAAIYFKNRFYPNVVLIFFCLLIYLLLEILIGGATALAEAVLGV 180

Query: 181 DLLPYFDEPYLSASLQEFWGRRWNLMTTRILRVAVYDPCRRVAGAAVGKRAAVMLAVAAT 240
           +LLP+FDEPYLS SLQ+FWGRRWNL+++RILR AV+DPCR++AG  VGK+ A ++AV AT
Sbjct: 181 ELLPFFDEPYLSDSLQDFWGRRWNLVSSRILRPAVFDPCRKLAGTVVGKKPAAIIAVMAT 240

Query: 241 FAVSGLMHELIYFYMGRMAPTWEVTCFFLLHGVCLPAEMAVKAAAGGRFRLPRAVSAPLT 300
           FAVSG+MHELIYFYMGR+ PTWEV+ FFL+HGVCL AEMAVK+A GGRFR PR VS  LT
Sbjct: 241 FAVSGVMHELIYFYMGRLTPTWEVSWFFLIHGVCLAAEMAVKSAVGGRFRPPRVVSGVLT 300

Query: 301 LGFVMVTSFWLFFPQFLRFKADVRMFEEYAALGALVKN 339
           +GFVM TSFWLFFPQ LRFKADVRM EEYAALGA VK+
Sbjct: 301 IGFVMATSFWLFFPQLLRFKADVRMLEEYAALGAFVKS 338

[Close]

BLAST of MS016071 vs. ExPASy TrEMBL
Match: A0A6J1KCL2 (acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111493090 PE=3 SV=1)

HSP 1 Score: 466.1 bits (1198), Expect = 1.3e-127
Identity = 241/338 (71.30%), Postives = 280/338 (82.84%), Query Frame = 0


Query: 1   MATEIGIFVQTVLLVCSSLCFCFSIRNSIPHGFLRFLLILPFFFLFLCIPLQFHTIHLQG 60
           MA EI IF+QT+L V SSLCFCFSIRNSIP+GFL+FLLILPFF LFL IPLQF T+HLQG
Sbjct: 1   MAAEILIFLQTLLQVSSSLCFCFSIRNSIPNGFLKFLLILPFFLLFLYIPLQFQTLHLQG 60

Query: 61  AIGFFIGWLGSFKLLLFAFGKGPLCSAPAAASLRRFLAVGSLPIEIPVHPPPAPAPDSPP 120
           AIGFFIGWL SFKLLLFAFGKG LCSA A++SL RFL VGSLPI+I  H P +  PD+  
Sbjct: 61  AIGFFIGWLASFKLLLFAFGKGSLCSAAASSSLSRFLLVGSLPIDIYDHDPDSTRPDTTS 120

Query: 121 VLSLTFPAKLGLLLLTFCAIYFRNYFNPYAVLVFYCLLIYLLLEILVGGAAALARTLLGV 180
           VL L+  +KL  L+L+  AIY +NYF+P   L+ +C +IYLLLEI++GGAAA+A+  LGV
Sbjct: 121 VLPLSLSSKLFFLILSIAAIYCKNYFHPNGTLIVFCFVIYLLLEIVLGGAAAVAKATLGV 180

Query: 181 DLLPYFDEPYLSASLQEFWGRRWNLMTTRILRVAVYDPCRRVAGAAVGKRAAVMLAVAAT 240
           +LLP FDEPYLS+SLQ+FWGRRWNLM +RILR++VYDPCR  A A VG++ A ++A+ AT
Sbjct: 181 ELLPCFDEPYLSSSLQDFWGRRWNLMVSRILRLSVYDPCRNFAAAIVGRKPAAIMAMTAT 240

Query: 241 FAVSGLMHELIYFYMGRMAPTWEVTCFFLLHGVCLPAEMAVKAAAGGRFRLPRAVSAPLT 300
           FAVSGLMHELIYFYMGR+APTWEVT FFL+HG  L  EMAVK A G RFR PR V+ PLT
Sbjct: 241 FAVSGLMHELIYFYMGRLAPTWEVTSFFLVHGFFLAVEMAVKNAVGERFRPPRIVTVPLT 300

Query: 301 LGFVMVTSFWLFFPQFLRFKADVRMFEEYAALGALVKN 339
           +GFVMVTSFWLFFPQF RFK DVRMFEE+AALGA VKN
Sbjct: 301 IGFVMVTSFWLFFPQFFRFKPDVRMFEEHAALGAFVKN 338

[Close]

BLAST of MS016071 vs. ExPASy TrEMBL
Match: A0A6J1GBB6 (acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111452615 PE=3 SV=1)

HSP 1 Score: 463.8 bits (1192), Expect = 6.3e-127
Identity = 240/338 (71.01%), Postives = 279/338 (82.54%), Query Frame = 0


Query: 1   MATEIGIFVQTVLLVCSSLCFCFSIRNSIPHGFLRFLLILPFFFLFLCIPLQFHTIHLQG 60
           MA EI IF+QT+L V SSLCFCFSIRNSIP+GFL+FLLILPFF LFL IPLQF T+HLQG
Sbjct: 1   MAAEILIFLQTLLQVSSSLCFCFSIRNSIPNGFLKFLLILPFFLLFLYIPLQFQTLHLQG 60

Query: 61  AIGFFIGWLGSFKLLLFAFGKGPLCSAPAAASLRRFLAVGSLPIEIPVHPPPAPAPDSPP 120
           AIGFFIGWL SFKLLLFAFGKGPLCSA A++SLRRFL VGSLPI+I  H   +  PD+  
Sbjct: 61  AIGFFIGWLASFKLLLFAFGKGPLCSAVASSSLRRFLLVGSLPIDIYDHDSASTRPDTNS 120

Query: 121 VLSLTFPAKLGLLLLTFCAIYFRNYFNPYAVLVFYCLLIYLLLEILVGGAAALARTLLGV 180
           VL L+  +KL  L L+  AIY +NYF+P   L+ +C +IYLLLEI++GGAAA+A+  LGV
Sbjct: 121 VLPLSLSSKLFFLSLSIAAIYCKNYFHPNGTLIVFCFVIYLLLEIVLGGAAAVAKATLGV 180

Query: 181 DLLPYFDEPYLSASLQEFWGRRWNLMTTRILRVAVYDPCRRVAGAAVGKRAAVMLAVAAT 240
           +LLP FDEPYLS+SLQ+FWGRRWNLM +RILR++VYDPCR  A A VG++ A ++A  AT
Sbjct: 181 ELLPCFDEPYLSSSLQDFWGRRWNLMVSRILRLSVYDPCRNFAAAIVGRKPAAIMATTAT 240

Query: 241 FAVSGLMHELIYFYMGRMAPTWEVTCFFLLHGVCLPAEMAVKAAAGGRFRLPRAVSAPLT 300
           FAVSGLMHELIYFY+GR+APTWEVT FFL+HG  L  EMAVK A G RFR PR V+ PLT
Sbjct: 241 FAVSGLMHELIYFYLGRLAPTWEVTSFFLVHGFFLAVEMAVKNAVGERFRPPRIVTVPLT 300

Query: 301 LGFVMVTSFWLFFPQFLRFKADVRMFEEYAALGALVKN 339
           +GFVMVTSFWLFFPQF RFK DVRMFEE+AA+GA VKN
Sbjct: 301 IGFVMVTSFWLFFPQFFRFKPDVRMFEEHAAMGAFVKN 338

[Close]

BLAST of MS016071 vs. TAIR 10
Match: AT3G51970.1 (acyl-CoA sterol acyl transferase 1 )

HSP 1 Score: 247.7 bits (631), Expect = 1.4e-65
Identity = 142/329 (43.16%), Postives = 196/329 (59.57%), Query Frame = 0


Query: 8   FVQTVLLVCSSLCFCFSIRNSIPHGFLRFLLILPFFFLFLCIPLQFHTIHLQGAIGFFIG 67
           F++   LV  SLC+ F I   +P G  R +L  P F +F  +P   +++HL G   FFI 
Sbjct: 4   FIKAWGLVIISLCYTFFIAKLVPKGIKRLILFFPVFLIFFIVPFLIYSLHLLGITAFFIA 63

Query: 68  WLGSFKLLLFAFGKGPLCSAPAAASLRRFLAVGSLPIEIPVHPPPAP--APDSPPVLSLT 127
           WL +FKLLLFA G+GPL S     SL  FLAV  LPI+I + P P    + +      L 
Sbjct: 64  WLANFKLLLFALGRGPLSSNHKPLSLPIFLAVSCLPIKIQLSPKPTKTHSHEGSTEGPLI 123

Query: 128 FPAKLGLLLLTFCAIYFRNYFNPYAVLVFYCLLIYLLLEILVGGAAALARTLLGVDLLPY 187
           +  K   ++L   A  +        VL  Y + IY  LEI++   AA  R +  ++L P 
Sbjct: 124 YTIKAVFVVLIIKAYEYSTKLPEKVVLTLYAIHIYFALEIILAATAAAVRAMSDLELEPQ 183

Query: 188 FDEPYLSASLQEFWGRRWNLMTTRILRVAVYDPCRRVAGAAVGKRAAVMLAVAATFAVSG 247
           F++PYL+ SLQ+FWGRRWNLM T ILR  VY+P  ++  + +G   + +LA   TF VSG
Sbjct: 184 FNKPYLATSLQDFWGRRWNLMVTGILRPTVYEPSLQLF-SVLGPNYSQILAAFGTFVVSG 243

Query: 248 LMHELIYFYMGRMAPTWEVTCFFLLHGVCLPAEMAVKAAAGGRFRLPRAVSAPLTLGFVM 307
           +MHELI+FYMGR+ P W++  FFL++G C   E+A+K    GR+R P+A+S  LTL FVM
Sbjct: 244 IMHELIFFYMGRLRPDWKMMWFFLINGFCTTVEIAIKKTINGRWRFPKAISQVLTLTFVM 303

Query: 308 VTSFWLFFPQFLRFKADVRMFEEYAALGA 335
           VT+ WLF P+F R     +  +EYAA+GA
Sbjct: 304 VTALWLFLPEFNRCNIVEKALDEYAAIGA 331

[Close]

BLAST of MS016071 vs. TAIR 10
Match: AT5G55350.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 212.6 bits (540), Expect = 4.9e-55
Identity = 135/339 (39.82%), Postives = 189/339 (55.75%), Query Frame = 0


Query: 1   MATEIGIFVQTVLLVCSSLCFCFSIRNSIPHGFLRFLLILPFFFLFLCIPLQFHTIHLQG 60
           M  E+   ++  +    S+ +C+     I  G  R L +LP   LFL +PL   ++H  G
Sbjct: 1   MEEELMSLIKVWVYAIISISYCYYTSTRIKSGVFRLLSVLPVCVLFLVLPLFVSSVHFSG 60

Query: 61  AIGFFIGWLGSFKLLLFAFGKGPLCSAPAAASLRRFLAVGSLPIEIPVHPPPAPAPDSPP 120
           +  FF+ WL +FKL+LF+F +GPL   P  ++L RF+     PI++  +P P        
Sbjct: 61  STAFFLSWLANFKLILFSFDQGPL--FPVPSNLSRFVCFTCFPIKLQQNPKP-----QNQ 120

Query: 121 VLSLTFPAKLGLLLLTFCAIYFRNYFNPYAVLVFYCLLIYLLLEILVGGAAALARTLLGV 180
           +    F  KL    +      ++ + +P  +LV Y L IYL  EIL+     L    L  
Sbjct: 121 MPKWGFAVKLAFFGVLLHMYEYKQHMSPTVLLVLYSLHIYLEYEILLAPLKVLLSISLWC 180

Query: 181 DLLPYFDEPYLSASLQEFWGRRWNLMTTRILRVAVYDPCRRVAGAAVGKRAAVMLAVAAT 240
           DL P+F+EPYLS SLQ+FWGRRWNLM   ILR AVY P R++AG  +    A+ L V A+
Sbjct: 181 DLEPHFNEPYLSTSLQDFWGRRWNLMVPAILRPAVYLPVRQMAGRKMNSDQALFLGVFAS 240

Query: 241 FAVSGLMHELIYFYMGRMAPTWEVTCFFLLHGVCLPAEMAVK-AAAGGRFRLPRAVSAPL 300
           F VSG++HELI+FY  R +PT EVT FF+LHGVC  AE A K      R+++ + VS  L
Sbjct: 241 FLVSGVVHELIFFYFTRESPTGEVTLFFVLHGVCTAAECAAKRTRLVRRWKVSQMVSRLL 300

Query: 301 TLGFVMVTSFWLFFPQFLRFKADVRMFEEYAALGALVKN 339
           T+GFV++T  WLFFP   R     R+ +E       VK+
Sbjct: 301 TVGFVVMTGGWLFFPHLARSGMIERLADEAFLFIGFVKH 332

[Close]

BLAST of MS016071 vs. TAIR 10
Match: AT5G55360.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 211.1 bits (536), Expect = 1.4e-54
Identity = 134/316 (42.41%), Postives = 183/316 (57.91%), Query Frame = 0


Query: 1   MATEIGIFVQTVLLVCSSLCFCFSIRNSIPHGFLRFLLILPFFFLFLCIPLQFHTIHLQG 60
           M  E+  F++  +    S+ +C+ +   I  G  R L +LP   LFL  PL F  +H  G
Sbjct: 1   MEEELKNFIKLWISAIISISYCYYLSTGIKAGVFRLLSVLPVCALFLVFPLFFSYVHFSG 60

Query: 61  AIGFFIGWLGSFKLLLFAFGKGPLCSAPAAASLRRFLAVGSLPIEIPVHPPPAPAPDSPP 120
            + FF+ WL +FKL+LF+F +GPL  +P   +L RF+ +   PI+    P   P   +  
Sbjct: 61  CMAFFLSWLANFKLILFSFDQGPL--SPLPRTLSRFICITCFPIK----PQQNPNIQNYK 120

Query: 121 VLSLTFPAKLGLLLLTFCAIYFRNYFNPYAVLVFYCLLIYLLLEILVGGAAALARTLLGV 180
           +    F  K+ + ++      ++ Y +P  +LVF  L I+L LEI+     AL    LG 
Sbjct: 121 IPIWLFAIKVVIFVVLLQMYEYKQYLSPALLLVFNSLHIFLELEIVFMLVKALVFITLGC 180

Query: 181 DLLPYFDEPYLSASLQEFWGRRWNLMTTRILRVAVYDPCRRVAGAAVGKRAAVMLAVAAT 240
           DL P  +EPYL+ SLQ+FWGRRWNLM   ILR AVY P RR+A   V    A+ L V A 
Sbjct: 181 DLEPQSNEPYLATSLQDFWGRRWNLMVPAILRPAVYLPARRMACRKVNSDQAMFLGVFAA 240

Query: 241 FAVSGLMHELIYFYMGRMAPTWEVTCFFLLHGVCLPAEMAVKAAAGGR--FRLPRAVSAP 300
           F VSG +HE+++FY+ R  PT EVT FFLLHGVC  AE+AVK +   R  +R+   VS  
Sbjct: 241 FLVSGAVHEMLFFYLTREVPTGEVTWFFLLHGVCTVAEVAVKKSTFVRRWWRVSPTVSRL 300

Query: 301 LTLGFVMVTSFWLFFP 315
           LT+GFV+VTS W FFP
Sbjct: 301 LTVGFVVVTSGWFFFP 310

[Close]

BLAST of MS016071 vs. TAIR 10
Match: AT5G55380.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 206.1 bits (523), Expect = 4.5e-53
Identity = 132/304 (43.42%), Postives = 172/304 (56.58%), Query Frame = 0


Query: 18  SLCFCFSIRNSIPHGFLRFLLILPFFFLFLCIPLQFHTIHLQGAIGFFIGWLGSFKLLLF 77
           SL +C+ I + +  G LR L ILP   LFL +PL    +H       F+ WL +FKLLLF
Sbjct: 18  SLSYCYYISSKLSKGVLRLLSILPVCILFLVLPLFLSCVHFCAISVLFLSWLANFKLLLF 77

Query: 78  AFGKGPLCSAPAAASLRRFLAVGSLPIEIPVHPPPAPAPD--SPPVLSLTFPAKLGLLLL 137
           AF +GPL   P    L RF+    LPI+I   P P   P+    P+       K+ +L +
Sbjct: 78  AFDEGPL--FPLPPKLSRFICFACLPIKIRQDPSPNAIPNLHPKPMPKWVLAVKILVLGV 137

Query: 138 TFCAIYFRNYFNPYAVLVFYCLLIYLLLEILVGGAAALARTLLGVDLLPYFDEPYLSASL 197
                 +R+    + VL  YCL IYL +E+++    A+  TLLG ++ P F+EPYL+ SL
Sbjct: 138 LLHVYEYRDGLPRFVVLALYCLHIYLEVELVLVFVGAVVSTLLGCNIEPVFNEPYLATSL 197

Query: 198 QEFWGRRWNLMTTRILRVAVYDPCRRVAGAAVGKRAAVMLAVAATFAVSGLMHELIYFYM 257
           Q+FW RRWNLM + +LR  V+ P +R     +    A+   V A+F VSGLMHEL+YFYM
Sbjct: 198 QDFWSRRWNLMVSAVLRSTVHIPVQRFFKRILSPDGAMFAGVMASFFVSGLMHELLYFYM 257

Query: 258 GRMAPTWEVTCFFLLHGVCLPAEMAVKAAAGGRFRLP-RAVSAPLTLGFVMVTSFWLFFP 317
            R  PTWEVTCFF+LHG     E+AVK       R P RAVS  + L FV VT  WLF  
Sbjct: 258 IRKPPTWEVTCFFVLHGAATATEIAVKRTQW--LRPPHRAVSGLVVLTFVSVTGVWLFLA 317

Query: 318 QFLR 319
           Q LR
Sbjct: 318 QVLR 317

[Close]

BLAST of MS016071 vs. TAIR 10
Match: AT5G55370.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 206.1 bits (523), Expect = 4.5e-53
Identity = 137/345 (39.71%), Postives = 192/345 (55.65%), Query Frame = 0


Query: 1   MATEIGIFVQTVLLVCSSLCFCFSIRNSIPHGFLRFLLILPFFFLFLCIPLQFHTIHLQG 60
           M  E+   ++  +    S+ +C+ I + I  G LR L +LP F +FL +PL F ++H   
Sbjct: 1   MEEELRNLIKVWISALISISYCYYISSKISKGVLRLLSLLPIFIIFLLLPLFFSSVHFCV 60

Query: 61  AIGFFIGWLGSFKLLLFAFGKGPLCSAPAAASLRRFLAVGSLPIEIPVHPPPAPAPDSPP 120
             GFF  WL +FKL LFAF + PL  +P  ++L RF      PI+I  +P      + P 
Sbjct: 61  ISGFFFTWLANFKLFLFAFDQEPL--SPLPSNLTRFFCFACFPIKINKNPSSNRIHNKP- 120

Query: 121 VLSLTFPAKLGLLLLTFCAIYFRNYFNP----YAVLVFYCLLIYLLLEILVGGAAALART 180
                +     LL+ +F    +RN ++     +A L  + + +YL  E+++    AL   
Sbjct: 121 --MSKWVLAFKLLIFSFLLHVYRNNYDSGLSRFAFLALFTIHVYLEAELILVFVGALMSM 180

Query: 181 LLGVDLLPYFDEPYLSASLQEFWGRRWNLMTTRILRVAVYDPCRRVAGAAVGKRAAVMLA 240
           LLG ++ P F++PYL+ SLQEFW RRWNLM   +LR AV+ P +R     +G   A    
Sbjct: 181 LLGCEMEPVFNDPYLATSLQEFWSRRWNLMVPAVLRPAVHIPVQRFCAPLLGLHRAFYAG 240

Query: 241 VAATFAVSGLMHELIYFYMGRMAPTWEVTCFFLLHGVCLPAEMAVKAAAGGRFR-LP--- 300
           + ATF VSGLMHELIYFY+ R +PTWEVTCFFLLHGV    E+A+K     R R LP   
Sbjct: 241 MLATFIVSGLMHELIYFYVIRKSPTWEVTCFFLLHGVVTCLEIAMK-----RMRWLPTPR 300

Query: 301 RAVSAPLTLGFVMVTSFWLFFPQFLRFKADVRMFEEYAALGALVK 338
           RAVS      F++VT+ WLF+PQ LR     R+  E   +  +VK
Sbjct: 301 RAVSGLAITVFLLVTAGWLFYPQMLRNDVHKRVISECLLVIDVVK 335

The following BLAST results are available for this feature:

BLAST of MS016071 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022152586.1	6.8e-184	99.41	acyl-CoA--sterol O-acyltransferase 1-like [Momordica charantia]	[more]
XP_038905685.1	2.1e-140	76.33	acyl-CoA--sterol O-acyltransferase 1-like [Benincasa hispida]	[more]
XP_022982637.1	4.0e-136	73.67	acyl-CoA--sterol O-acyltransferase 1-like [Cucurbita maxima]	[more]
XP_023528035.1	8.4e-134	72.49	acyl-CoA--sterol O-acyltransferase 1-like [Cucurbita pepo subsp. pepo]	[more]
XP_022934181.1	8.4e-134	72.78	acyl-CoA--sterol O-acyltransferase 1-like [Cucurbita moschata] >KAG6581558.1 Acy...	[more]

BLAST of MS016071 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SV07	1.9e-64	43.16	Acyl-CoA--sterol O-acyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=ASAT1 PE...	[more]
Q9XGY6	3.5e-58	42.26	Long-chain-alcohol O-fatty-acyltransferase OS=Simmondsia chinensis OX=3999 PE=1 ...	[more]
Q9FJ75	6.8e-54	39.82	Probable long-chain-alcohol O-fatty-acyltransferase 4 OS=Arabidopsis thaliana OX...	[more]
Q9FJ74	2.0e-53	42.41	Probable long-chain-alcohol O-fatty-acyltransferase 3 OS=Arabidopsis thaliana OX...	[more]
Q9FJ72	6.4e-52	43.42	Probable long-chain-alcohol O-fatty-acyltransferase 1 OS=Arabidopsis thaliana OX...	[more]

BLAST of MS016071 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DI64	3.3e-184	99.41	acyl-CoA--sterol O-acyltransferase 1-like OS=Momordica charantia OX=3673 GN=LOC1...	[more]
A0A6J1IX47	1.9e-136	73.67	acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita maxima OX=3661 GN=LOC1114...	[more]
A0A6J1F144	4.1e-134	72.78	acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita moschata OX=3662 GN=LOC11...	[more]
A0A6J1KCL2	1.3e-127	71.30	acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita maxima OX=3661 GN=LOC1114...	[more]
A0A6J1GBB6	6.3e-127	71.01	acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita moschata OX=3662 GN=LOC11...	[more]

BLAST of MS016071 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G51970.1	1.4e-65	43.16	acyl-CoA sterol acyl transferase 1	[more]
AT5G55350.1	4.9e-55	39.82	MBOAT (membrane bound O-acyl transferase) family protein	[more]
AT5G55360.1	1.4e-54	42.41	MBOAT (membrane bound O-acyl transferase) family protein	[more]
AT5G55380.1	4.5e-53	43.42	MBOAT (membrane bound O-acyl transferase) family protein	[more]
AT5G55370.1	4.5e-53	39.71	MBOAT (membrane bound O-acyl transferase) family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR017088	Wax synthase, Magnoliopsida	PIRSF	PIRSF037006	Wax_synthase	coord: 1..338 e-value: 9.5E-111 score: 368.2
IPR032805	Wax synthase domain	PFAM	PF13813	MBOAT_2	coord: 184..269 e-value: 5.7E-17 score: 61.8
IPR044851	Wax synthase	PANTHER	PTHR31595	LONG-CHAIN-ALCOHOL O-FATTY-ACYLTRANSFERASE 3-RELATED	coord: 3..338
None	No IPR available	PANTHER	PTHR31595:SF46	ACYL-COA--STEROL O-ACYLTRANSFERASE 1	coord: 3..338

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS016071.1	MS016071.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006629	lipid metabolic process
molecular_function	GO:0008374	O-acyltransferase activity