MS016069 (gene) Bitter gourd (TR) v1

Overview


Name	MS016069
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Hydroxymethylbilane synthase
Location	scaffold9_2: 157865 .. 160510 (-)
RNA-Seq Expression	MS016069
Synteny	MS016069

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGTGTTTTCTCTTCTCCTCCTATAACCCACTCTCCAATGGCTCGTCCCTGTAATGTTGGGTCCCTCTCCCTTCTTGGGTTCTCCTCACTTTCCCTTAAACCTCCGGCTTTCTGTAATGGGGGCAAGAAATTTGGTGGTTTAGGGCTCGTCAGAGCCTCTGTTGCAGTGGAGCAGCAGACGGAGAAGACCAAGGTTGCTCTCGTCAGAATTGGGACCAGAGGAAGGTGAATTTCTGTTTGTTGCCTTCTTCTGCTTTCTGGGTCTTTCTTAGTTTTCTCTTTTAGTTTCCAATTTATGTTTTACCACTGATTTGTGTTAGCAAATGTGGCATTGAATTGTTGTTGAAGCATTTTGATCGAGAAAATAGTCTTTGAAACGTGATTTGGTTATGGTATCTAGTGTAATTGTAAAGAGTTCACTAAGTTTTTATCATGTTTGCAGTCTTTGCACTTGAATCCTCTAATTCTAGCGCTGCCAAAACTGATATTTATCACTCTTATCCCTACTGAAGTAGTACCGAGATAGAGAGATTTTACAACCCCCCTCTCCCCTCAAATGGGTTGAGTGTGTAAGTTGCATTTTTCCATGGTTGTAAAATTAAGAATTTGGGCTGTCGGTTTGCCTTCTGAAAAGGGTCCTCAAACCTTTCGGCCAGAGCTCTTCTTTGGTTGATTTGGAAACAGAGGAATTTTTGAAGATAAAATGAATATGAAGAAATCTTTTTGGAGTAGTGTGCAGCATACTATGTCTACTAGAAATTATTTTGTAATTACAGCGTCTTCATGATTATTAATGATTGGTTGACCATTCTCTTGTAGTTTCGTGGGCAGGGTTTCCTCTGCCCTGGCCCTGTGGTGGAAAACTAGACTATGTCTATAAACATGATGTACTTCATTTTCTAGCTGGCTATATGCTTGTTTGATCTAAATCTTGTGCTGGTTTGCTGTATCTTTTTCTGTGTCATATATATACTTCTGTGTGTAGGACAGCCCCTTAGCACTTGCCCAGGCTTATGAGACGAGGGACAAACTCATGGCTATCCATCCCGAGCTAGCTGAAGATGGGGCCATTCAGATCATTATAATCAAAACAACCGGCGACAAAATACTGTCGCAGCCACTTGCAGACATTGGTGGGAAAGGCTTATTTACTAAAGAGATCGATGATGCACTTATTAATAGTGACATAGACATTGCTGTTCACTCAATGAAAGATGTACCGACTTACTTACCTGAAAAAACGATCCTCCCATGTAATCTTCCGAGGGAGGATGTCCGTGATGCATTCATTTCTTTGAGTGCGGGTTCGCTTGCTGAGCTTCCAGCTGGAAGTGTCATTGGTACAGCTTCACTGAGAAGAAAGTCTCAGCTACTGAATAGATATCCATCCCTCAAAGTAAGAATGTTGTGTCTTTTAATGGAAAAACATTGCGTATACATCACTATGGGAGTTAATAAGTTATCGATCTCAGCATCTCATGTGTGAAATTGGCTCCTGAAAGATATGATAGAAAGAATGTCTCTTTGGTTTCTTTTCTACCTTGGTTGATTTTTTAAAATGTTGCTCTTGTCAGTCAACATGTTCTCTCAACAAATTCCATTCTCTCTTCTTATTTATTGCTGATACTATGAAGAGTTACCAGACTATGTTTGACGATTGACGGTTATTTTCTTATTCGAGACGGAGATTCCCTGCTGCCCCTACTCTTTTCCTTTTAACTTCCCAAATCTATCTTCTACTTGAACATAGTGGACCTGAGAGCTTTTTTGATATCTCTGTTGGTTGTGCTCATTTTCCATGATTCAACTTAGCACATCTTCTGTCTCTTGATGGAACTGTTTGCAACTACTTATGATTTTAAGGATGTTAAAGAATCTTAAATTTTCAATATTGTAGATTGATTCTGACATTCACTCCCTGTTTTTGGTCTGTGGGTGTCATTTTCAGGTGCTGGAGAATTTTCGGGGCAATGTCCAAACAAGGTTGAGAAAACTGAATGAAGGAGTGGTCCAAGCAACACTATTGGCACTAGCTGGACTTCGGCGTTTAAACATGACAGAAAACGTTACTTCAGTTCTCTCAATTGATGAAATGCTTCCAGCCGTGGCGCAGGGAGCAATTGGTATTGCCTGTCGAAGCAACGATGATAAAATGGTATGTTTTCATCGGCTATGTAAATCAATCCTTACCCGTTGCTTGTGTTAGTTTGGGAAATGCCATTTATAACTACATCCTGTTTGGCTTTTCATTTGCAGGCCAATTACTTGGCCTCATTAAACCATGAGGAAACCAGACTGGCTGTTGTTTGTGAGAGAGCTTTCCTCGAGACGCTTGATGGATCTTGTCGAACTCCAATCGCAGGATATGCTTCCAAAGATGAAGATGGTAACTGTATATTTAAAGGGTTGGTGGCCTCCCCGGATGGAACCCGAGGTACAAACGACATCCATGTTTAGATCAAACGACTTTTACTCTCATTTTTGTCAGCTTCATCTTCCAACCCTTACCAATGTTTCTTCCTCTTTCATTTCAGTTCTCGAAACTTCTCGAAGAGGTGCATACGCCATCGACGATATGGTTGCAATGGGAAAGGATGCCGGAAAGGAGCTTCTTTCTCGAGCAGGTCCCGGTTTTTTCGATAGC

mRNA sequence

ATGGGTGTTTTCTCTTCTCCTCCTATAACCCACTCTCCAATGGCTCGTCCCTGTAATGTTGGGTCCCTCTCCCTTCTTGGGTTCTCCTCACTTTCCCTTAAACCTCCGGCTTTCTGTAATGGGGGCAAGAAATTTGGTGGTTTAGGGCTCGTCAGAGCCTCTGTTGCAGTGGAGCAGCAGACGGAGAAGACCAAGGTTGCTCTCGTCAGAATTGGGACCAGAGGAAGCCCCTTAGCACTTGCCCAGGCTTATGAGACGAGGGACAAACTCATGGCTATCCATCCCGAGCTAGCTGAAGATGGGGCCATTCAGATCATTATAATCAAAACAACCGGCGACAAAATACTGTCGCAGCCACTTGCAGACATTGGTGGGAAAGGCTTATTTACTAAAGAGATCGATGATGCACTTATTAATAGTGACATAGACATTGCTGTTCACTCAATGAAAGATGTACCGACTTACTTACCTGAAAAAACGATCCTCCCATGTAATCTTCCGAGGGAGGATGTCCGTGATGCATTCATTTCTTTGAGTGCGGGTTCGCTTGCTGAGCTTCCAGCTGGAAGTGTCATTGGTACAGCTTCACTGAGAAGAAAGTCTCAGCTACTGAATAGATATCCATCCCTCAAAGTGCTGGAGAATTTTCGGGGCAATGTCCAAACAAGGTTGAGAAAACTGAATGAAGGAGTGGTCCAAGCAACACTATTGGCACTAGCTGGACTTCGGCGTTTAAACATGACAGAAAACGTTACTTCAGTTCTCTCAATTGATGAAATGCTTCCAGCCGTGGCGCAGGGAGCAATTGGTATTGCCTGTCGAAGCAACGATGATAAAATGGCCAATTACTTGGCCTCATTAAACCATGAGGAAACCAGACTGGCTGTTGTTTGTGAGAGAGCTTTCCTCGAGACGCTTGATGGATCTTGTCGAACTCCAATCGCAGGATATGCTTCCAAAGATGAAGATGGTAACTGTATATTTAAAGGGTTGGTGGCCTCCCCGGATGGAACCCGAGTTCTCGAAACTTCTCGAAGAGGTGCATACGCCATCGACGATATGGTTGCAATGGGAAAGGATGCCGGAAAGGAGCTTCTTTCTCGAGCAGGTCCCGGTTTTTTCGATAGC

Coding sequence (CDS)

ATGGGTGTTTTCTCTTCTCCTCCTATAACCCACTCTCCAATGGCTCGTCCCTGTAATGTTGGGTCCCTCTCCCTTCTTGGGTTCTCCTCACTTTCCCTTAAACCTCCGGCTTTCTGTAATGGGGGCAAGAAATTTGGTGGTTTAGGGCTCGTCAGAGCCTCTGTTGCAGTGGAGCAGCAGACGGAGAAGACCAAGGTTGCTCTCGTCAGAATTGGGACCAGAGGAAGCCCCTTAGCACTTGCCCAGGCTTATGAGACGAGGGACAAACTCATGGCTATCCATCCCGAGCTAGCTGAAGATGGGGCCATTCAGATCATTATAATCAAAACAACCGGCGACAAAATACTGTCGCAGCCACTTGCAGACATTGGTGGGAAAGGCTTATTTACTAAAGAGATCGATGATGCACTTATTAATAGTGACATAGACATTGCTGTTCACTCAATGAAAGATGTACCGACTTACTTACCTGAAAAAACGATCCTCCCATGTAATCTTCCGAGGGAGGATGTCCGTGATGCATTCATTTCTTTGAGTGCGGGTTCGCTTGCTGAGCTTCCAGCTGGAAGTGTCATTGGTACAGCTTCACTGAGAAGAAAGTCTCAGCTACTGAATAGATATCCATCCCTCAAAGTGCTGGAGAATTTTCGGGGCAATGTCCAAACAAGGTTGAGAAAACTGAATGAAGGAGTGGTCCAAGCAACACTATTGGCACTAGCTGGACTTCGGCGTTTAAACATGACAGAAAACGTTACTTCAGTTCTCTCAATTGATGAAATGCTTCCAGCCGTGGCGCAGGGAGCAATTGGTATTGCCTGTCGAAGCAACGATGATAAAATGGCCAATTACTTGGCCTCATTAAACCATGAGGAAACCAGACTGGCTGTTGTTTGTGAGAGAGCTTTCCTCGAGACGCTTGATGGATCTTGTCGAACTCCAATCGCAGGATATGCTTCCAAAGATGAAGATGGTAACTGTATATTTAAAGGGTTGGTGGCCTCCCCGGATGGAACCCGAGTTCTCGAAACTTCTCGAAGAGGTGCATACGCCATCGACGATATGGTTGCAATGGGAAAGGATGCCGGAAAGGAGCTTCTTTCTCGAGCAGGTCCCGGTTTTTTCGATAGC

Protein sequence

MGVFSSPPITHSPMARPCNVGSLSLLGFSSLSLKPPAFCNGGKKFGGLGLVRASVAVEQQTEKTKVALVRIGTRGSPLALAQAYETRDKLMAIHPELAEDGAIQIIIIKTTGDKILSQPLADIGGKGLFTKEIDDALINSDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSAGSLAELPAGSVIGTASLRRKSQLLNRYPSLKVLENFRGNVQTRLRKLNEGVVQATLLALAGLRRLNMTENVTSVLSIDEMLPAVAQGAIGIACRSNDDKMANYLASLNHEETRLAVVCERAFLETLDGSCRTPIAGYASKDEDGNCIFKGLVASPDGTRVLETSRRGAYAIDDMVAMGKDAGKELLSRAGPGFFDS

Homology

[Close]

BLAST of MS016069 vs. NCBI nr
Match: XP_022152701.1 (porphobilinogen deaminase, chloroplastic [Momordica charantia])

HSP 1 Score: 728.0 bits (1878), Expect = 4.1e-206
Identity = 374/376 (99.47%), Postives = 374/376 (99.47%), Query Frame = 0


Query: 1   MGVFSSPPITHSPMARPCNVGSLSLLGFSSLSLKPPAFCNGGKKFGGLGLVRASVAVEQQ 60
           MGVFSSPPITHSPMARPCNVGSLS LGFSSLSLKPPAFCNGGKKFGGLGLVRASVAVEQQ
Sbjct: 1   MGVFSSPPITHSPMARPCNVGSLSPLGFSSLSLKPPAFCNGGKKFGGLGLVRASVAVEQQ 60

Query: 61  TEKTKVALVRIGTRGSPLALAQAYETRDKLMAIHPELAEDGAIQIIIIKTTGDKILSQPL 120
           TEKTKVALVRIGTRGSPLALAQAYETRDKLMAIHPELAEDGAIQIIIIKTTGDKILSQPL
Sbjct: 61  TEKTKVALVRIGTRGSPLALAQAYETRDKLMAIHPELAEDGAIQIIIIKTTGDKILSQPL 120

Query: 121 ADIGGKGLFTKEIDDALINSDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSA 180
           ADIGGKGLFTKEIDDALINSDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSA
Sbjct: 121 ADIGGKGLFTKEIDDALINSDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSA 180

Query: 181 GSLAELPAGSVIGTASLRRKSQLLNRYPSLKVLENFRGNVQTRLRKLNEGVVQATLLALA 240
           GSLAELPAGSVIGTASLRRKSQLLNRYPSLKVLENFRGNVQTRLRKLNEGVVQATLLALA
Sbjct: 181 GSLAELPAGSVIGTASLRRKSQLLNRYPSLKVLENFRGNVQTRLRKLNEGVVQATLLALA 240

Query: 241 GLRRLNMTENVTSVLSIDEMLPAVAQGAIGIACRSNDDKMANYLASLNHEETRLAVVCER 300
           GLRRLNMTENVTSVLSIDEMLPAVAQGAIGIACRSNDDKMANYLASLNHEETRLAVVCER
Sbjct: 241 GLRRLNMTENVTSVLSIDEMLPAVAQGAIGIACRSNDDKMANYLASLNHEETRLAVVCER 300

Query: 301 AFLETLDGSCRTPIAGYASKDEDGNCIFKGLVASPDGTRVLETSRRGAYAIDDMVAMGKD 360
           AFLE LDGSCRTPIAGYASKDEDGNCIFKGLVASPDGTRVLETSRRGAYAIDDMVAMGKD
Sbjct: 301 AFLEMLDGSCRTPIAGYASKDEDGNCIFKGLVASPDGTRVLETSRRGAYAIDDMVAMGKD 360

Query: 361 AGKELLSRAGPGFFDS 377
           AGKELLSRAGPGFFDS
Sbjct: 361 AGKELLSRAGPGFFDS 376

[Close]

BLAST of MS016069 vs. NCBI nr
Match: XP_038906286.1 (porphobilinogen deaminase, chloroplastic [Benincasa hispida])

HSP 1 Score: 694.1 bits (1790), Expect = 6.6e-196
Identity = 353/376 (93.88%), Postives = 365/376 (97.07%), Query Frame = 0


Query: 1   MGVFSSPPITHSPMARPCNVGSLSLLGFSSLSLKPPAFCNGGKKFGGLGLVRASVAVEQQ 60
           MGV SSP ++HSPM RP NVGSLSLLGFSSLSLKPPAF NG KKF G GL+RASVAVEQQ
Sbjct: 1   MGVLSSPSVSHSPMPRPRNVGSLSLLGFSSLSLKPPAFSNGAKKFHGFGLIRASVAVEQQ 60

Query: 61  TEKTKVALVRIGTRGSPLALAQAYETRDKLMAIHPELAEDGAIQIIIIKTTGDKILSQPL 120
           TEKTKVALVRIGTRGSPLALAQA+ETRDKLMA HPELAEDGAIQI++IKTTGDKILSQPL
Sbjct: 61  TEKTKVALVRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPL 120

Query: 121 ADIGGKGLFTKEIDDALINSDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSA 180
           ADIGGKGLFTKEIDDALIN DIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSA
Sbjct: 121 ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSA 180

Query: 181 GSLAELPAGSVIGTASLRRKSQLLNRYPSLKVLENFRGNVQTRLRKLNEGVVQATLLALA 240
           GSLAELPAGS+IGTASLRRKSQLLNRYPSLKVLENFRGNVQTRLRKLNEGVVQATLLALA
Sbjct: 181 GSLAELPAGSIIGTASLRRKSQLLNRYPSLKVLENFRGNVQTRLRKLNEGVVQATLLALA 240

Query: 241 GLRRLNMTENVTSVLSIDEMLPAVAQGAIGIACRSNDDKMANYLASLNHEETRLAVVCER 300
           GLRRLNMTENVTS+LSIDEMLPAVAQGAIGIACR++DDKMANYLASLNHEETRLAVVCER
Sbjct: 241 GLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRTDDDKMANYLASLNHEETRLAVVCER 300

Query: 301 AFLETLDGSCRTPIAGYASKDEDGNCIFKGLVASPDGTRVLETSRRGAYAIDDMVAMGKD 360
           AFLETLDGSCRTPIAGYAS+DEDGNCIFKGLVASPDGTRVLETSRRG YAIDDM+AMGKD
Sbjct: 301 AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAIDDMIAMGKD 360

Query: 361 AGKELLSRAGPGFFDS 377
           AGKELLSRAGPGFFDS
Sbjct: 361 AGKELLSRAGPGFFDS 376

[Close]

BLAST of MS016069 vs. NCBI nr
Match: XP_022949290.1 (porphobilinogen deaminase, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 689.9 bits (1779), Expect = 1.2e-194
Identity = 352/376 (93.62%), Postives = 362/376 (96.28%), Query Frame = 0


Query: 1   MGVFSSPPITHSPMARPCNVGSLSLLGFSSLSLKPPAFCNGGKKFGGLGLVRASVAVEQQ 60
           MGVFSSP I+H PMARPCN+GSLS  GFSSLSLKPPAF  GGKKF G GL+RASVAVEQQ
Sbjct: 1   MGVFSSPSISHYPMARPCNLGSLSRHGFSSLSLKPPAFSTGGKKFHGFGLIRASVAVEQQ 60

Query: 61  TEKTKVALVRIGTRGSPLALAQAYETRDKLMAIHPELAEDGAIQIIIIKTTGDKILSQPL 120
            EKTKVALVRIGTRGSPLALAQA+ETR+KLMAIHPELAEDGAIQI+IIKTTGDKILSQPL
Sbjct: 61  AEKTKVALVRIGTRGSPLALAQAHETREKLMAIHPELAEDGAIQIVIIKTTGDKILSQPL 120

Query: 121 ADIGGKGLFTKEIDDALINSDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSA 180
           ADIGGKGLFTKEIDDALIN DIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLS 
Sbjct: 121 ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSV 180

Query: 181 GSLAELPAGSVIGTASLRRKSQLLNRYPSLKVLENFRGNVQTRLRKLNEGVVQATLLALA 240
           GSLAELPAGSVIGTASLRRKSQLLNRYPSL VLENFRGNVQTRLRKLNEGVVQATLLALA
Sbjct: 181 GSLAELPAGSVIGTASLRRKSQLLNRYPSLNVLENFRGNVQTRLRKLNEGVVQATLLALA 240

Query: 241 GLRRLNMTENVTSVLSIDEMLPAVAQGAIGIACRSNDDKMANYLASLNHEETRLAVVCER 300
           GLRRLNMTE VTS+LSIDEMLPAVAQGAIGIACRS+DDKMANYLASLNHEETRLAVVCER
Sbjct: 241 GLRRLNMTETVTSILSIDEMLPAVAQGAIGIACRSDDDKMANYLASLNHEETRLAVVCER 300

Query: 301 AFLETLDGSCRTPIAGYASKDEDGNCIFKGLVASPDGTRVLETSRRGAYAIDDMVAMGKD 360
           AFLETLDGSCRTPIAGYAS+DEDGNCIFKGLVASPDGTRVLETSRRG YAIDDM+AMGKD
Sbjct: 301 AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAIDDMIAMGKD 360

Query: 361 AGKELLSRAGPGFFDS 377
           AGKELLSRAGPGFFDS
Sbjct: 361 AGKELLSRAGPGFFDS 376

[Close]

BLAST of MS016069 vs. NCBI nr
Match: KAG7036601.1 (Porphobilinogen deaminase, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 688.7 bits (1776), Expect = 2.8e-194
Identity = 351/376 (93.35%), Postives = 362/376 (96.28%), Query Frame = 0


Query: 1   MGVFSSPPITHSPMARPCNVGSLSLLGFSSLSLKPPAFCNGGKKFGGLGLVRASVAVEQQ 60
           MGVFSSP I+H PMARPCN+GSLS  GFSSLSLKPPAF  GGKKF G GL+RASVAVEQQ
Sbjct: 1   MGVFSSPSISHYPMARPCNLGSLSRHGFSSLSLKPPAFSTGGKKFHGFGLIRASVAVEQQ 60

Query: 61  TEKTKVALVRIGTRGSPLALAQAYETRDKLMAIHPELAEDGAIQIIIIKTTGDKILSQPL 120
            EKTKVALVRIGTRGSPLALAQA+ETR+KLMAIHPELAEDGAIQI+IIKTTGDKILSQPL
Sbjct: 61  AEKTKVALVRIGTRGSPLALAQAHETREKLMAIHPELAEDGAIQIVIIKTTGDKILSQPL 120

Query: 121 ADIGGKGLFTKEIDDALINSDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSA 180
           ADIGGKGLFTKEIDDALIN DIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLS 
Sbjct: 121 ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSV 180

Query: 181 GSLAELPAGSVIGTASLRRKSQLLNRYPSLKVLENFRGNVQTRLRKLNEGVVQATLLALA 240
           GSLAELPAGSVIGTASLRRKSQLLNRYPSL VLENFRGNVQTRLRKLNEGVVQATLLALA
Sbjct: 181 GSLAELPAGSVIGTASLRRKSQLLNRYPSLNVLENFRGNVQTRLRKLNEGVVQATLLALA 240

Query: 241 GLRRLNMTENVTSVLSIDEMLPAVAQGAIGIACRSNDDKMANYLASLNHEETRLAVVCER 300
           GLRRLNMTE VTS+LSIDEMLPAVAQGAIGIACRS+DDKMANYL+SLNHEETRLAVVCER
Sbjct: 241 GLRRLNMTETVTSILSIDEMLPAVAQGAIGIACRSDDDKMANYLSSLNHEETRLAVVCER 300

Query: 301 AFLETLDGSCRTPIAGYASKDEDGNCIFKGLVASPDGTRVLETSRRGAYAIDDMVAMGKD 360
           AFLETLDGSCRTPIAGYAS+DEDGNCIFKGLVASPDGTRVLETSRRG YAIDDM+AMGKD
Sbjct: 301 AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAIDDMIAMGKD 360

Query: 361 AGKELLSRAGPGFFDS 377
           AGKELLSRAGPGFFDS
Sbjct: 361 AGKELLSRAGPGFFDS 376

[Close]

BLAST of MS016069 vs. NCBI nr
Match: XP_023523547.1 (porphobilinogen deaminase, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 687.6 bits (1773), Expect = 6.1e-194
Identity = 351/376 (93.35%), Postives = 361/376 (96.01%), Query Frame = 0


Query: 1   MGVFSSPPITHSPMARPCNVGSLSLLGFSSLSLKPPAFCNGGKKFGGLGLVRASVAVEQQ 60
           MGVFSSP I+H PMARPCN+GSLS  GFSSLSLKPPAF  G KKF G GL+RASVAVEQQ
Sbjct: 18  MGVFSSPSISHYPMARPCNLGSLSRHGFSSLSLKPPAFSTGAKKFHGFGLIRASVAVEQQ 77

Query: 61  TEKTKVALVRIGTRGSPLALAQAYETRDKLMAIHPELAEDGAIQIIIIKTTGDKILSQPL 120
            EKTKVALVRIGTRGSPLALAQA+ETR+KLMAIHPELAEDGAIQI+IIKTTGDKILSQPL
Sbjct: 78  AEKTKVALVRIGTRGSPLALAQAHETREKLMAIHPELAEDGAIQIVIIKTTGDKILSQPL 137

Query: 121 ADIGGKGLFTKEIDDALINSDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSA 180
           ADIGGKGLFTKEIDDALIN DIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLS 
Sbjct: 138 ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSV 197

Query: 181 GSLAELPAGSVIGTASLRRKSQLLNRYPSLKVLENFRGNVQTRLRKLNEGVVQATLLALA 240
           GSLAELPAGSVIGTASLRRKSQLLNRYPSL VLENFRGNVQTRLRKLNEGVVQATLLALA
Sbjct: 198 GSLAELPAGSVIGTASLRRKSQLLNRYPSLNVLENFRGNVQTRLRKLNEGVVQATLLALA 257

Query: 241 GLRRLNMTENVTSVLSIDEMLPAVAQGAIGIACRSNDDKMANYLASLNHEETRLAVVCER 300
           GLRRLNMTE VTS+LSIDEMLPAVAQGAIGIACRS+DDKMANYLASLNHEETRLAVVCER
Sbjct: 258 GLRRLNMTETVTSILSIDEMLPAVAQGAIGIACRSDDDKMANYLASLNHEETRLAVVCER 317

Query: 301 AFLETLDGSCRTPIAGYASKDEDGNCIFKGLVASPDGTRVLETSRRGAYAIDDMVAMGKD 360
           AFLETLDGSCRTPIAGYAS+DEDGNCIFKGLVASPDGTRVLETSRRG YAIDDM+AMGKD
Sbjct: 318 AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAIDDMIAMGKD 377

Query: 361 AGKELLSRAGPGFFDS 377
           AGKELLSRAGPGFFDS
Sbjct: 378 AGKELLSRAGPGFFDS 393

[Close]

BLAST of MS016069 vs. ExPASy Swiss-Prot
Match: Q43082 (Porphobilinogen deaminase, chloroplastic OS=Pisum sativum OX=3888 GN=HEMC PE=1 SV=1)

HSP 1 Score: 561.2 bits (1445), Expect = 8.7e-159
Identity = 286/349 (81.95%), Postives = 316/349 (90.54%), Query Frame = 0


Query: 28  FSSLSLKPPAFCNGGKKFGGLGLVRASVAVEQQTEKTKVALVRIGTRGSPLALAQAYETR 87
           FSS    P + C           +RAS+AVEQQT++ K AL+RIGTRGSPLALAQA+ETR
Sbjct: 31  FSSFKTSPFSKCR----------IRASLAVEQQTQQNKTALIRIGTRGSPLALAQAHETR 90

Query: 88  DKLMAIHPELAEDGAIQIIIIKTTGDKILSQPLADIGGKGLFTKEIDDALINSDIDIAVH 147
           DKLMA H ELAE+GAIQI+IIKTTGDKILSQPLADIGGKGLFTKEID+ALIN DIDIAVH
Sbjct: 91  DKLMASHTELAEEGAIQIVIIKTTGDKILSQPLADIGGKGLFTKEIDEALINGDIDIAVH 150

Query: 148 SMKDVPTYLPEKTILPCNLPREDVRDAFISLSAGSLAELPAGSVIGTASLRRKSQLLNRY 207
           SMKDVPTYLPE+TILPCNLPREDVRDAFISLSA SLA+LPAGSVIGTASLRRKSQ+L+RY
Sbjct: 151 SMKDVPTYLPEETILPCNLPREDVRDAFISLSAASLADLPAGSVIGTASLRRKSQILHRY 210

Query: 208 PSLKVLENFRGNVQTRLRKLNEGVVQATLLALAGLRRLNMTENVTSVLSIDEMLPAVAQG 267
           PSL V +NFRGNVQTRLRKL+EGVV+ATLLALAGL+RLNMTENVTS LSID+MLPAVAQG
Sbjct: 211 PSLTVQDNFRGNVQTRLRKLSEGVVKATLLALAGLKRLNMTENVTSTLSIDDMLPAVAQG 270

Query: 268 AIGIACRSNDDKMANYLASLNHEETRLAVVCERAFLETLDGSCRTPIAGYASKDEDGNCI 327
           AIGIACRSNDDKMA YLASLNHEETRLA+ CERAFL TLDGSCRTPIAGYAS+D+DGNC+
Sbjct: 271 AIGIACRSNDDKMAEYLASLNHEETRLAISCERAFLTTLDGSCRTPIAGYASRDKDGNCL 330

Query: 328 FKGLVASPDGTRVLETSRRGAYAIDDMVAMGKDAGKELLSRAGPGFFDS 377
           F+GLVASPDGTRVLETSR G+Y  +DM+ +GKDAG+ELLSRAGPGFF+S
Sbjct: 331 FRGLVASPDGTRVLETSRIGSYTYEDMMKIGKDAGEELLSRAGPGFFNS 369

[Close]

BLAST of MS016069 vs. ExPASy Swiss-Prot
Match: Q43316 (Porphobilinogen deaminase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=HEMC PE=1 SV=1)

HSP 1 Score: 552.7 bits (1423), Expect = 3.1e-156
Identity = 284/368 (77.17%), Postives = 318/368 (86.41%), Query Frame = 0


Query: 9   ITHSPMAR--PCNVGSLSLLGFSSLSLKPPAFCNGGKKFGGLGLVRASVAVEQQTEKTKV 68
           +T  P +R   C++GS+S +GFS   +  PA     +K    G V+A VAVEQ   KT+ 
Sbjct: 16  LTRQPSSRVNTCSLGSVSAIGFSLPQISSPALGKCRRKQSSSGFVKACVAVEQ---KTRT 75

Query: 69  ALVRIGTRGSPLALAQAYETRDKLMAIHPELAEDGAIQIIIIKTTGDKILSQPLADIGGK 128
           A++RIGTRGSPLALAQAYETR+KL   HPEL EDGAI I IIKTTGDKILSQPLADIGGK
Sbjct: 76  AIIRIGTRGSPLALAQAYETREKLKKKHPELVEDGAIHIEIIKTTGDKILSQPLADIGGK 135

Query: 129 GLFTKEIDDALINSDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSAGSLAEL 188
           GLFTKEID+ALIN  IDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFI L+A +LAEL
Sbjct: 136 GLFTKEIDEALINGHIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFICLTAATLAEL 195

Query: 189 PAGSVIGTASLRRKSQLLNRYPSLKVLENFRGNVQTRLRKLNEGVVQATLLALAGLRRLN 248
           PAGSV+GTASLRRKSQ+L++YP+L V ENFRGNVQTRL KL  G VQATLLALAGL+RL+
Sbjct: 196 PAGSVVGTASLRRKSQILHKYPALHVEENFRGNVQTRLSKLQGGKVQATLLALAGLKRLS 255

Query: 249 MTENVTSVLSIDEMLPAVAQGAIGIACRSNDDKMANYLASLNHEETRLAVVCERAFLETL 308
           MTENV S+LS+DEMLPAVAQGAIGIACR++DDKMA YLASLNHEETRLA+ CERAFLETL
Sbjct: 256 MTENVASILSLDEMLPAVAQGAIGIACRTDDDKMATYLASLNHEETRLAISCERAFLETL 315

Query: 309 DGSCRTPIAGYASKDEDGNCIFKGLVASPDGTRVLETSRRGAYAIDDMVAMGKDAGKELL 368
           DGSCRTPIAGYASKDE+GNCIF+GLVASPDGT+VLETSR+G Y  +DMV MGKDAG+ELL
Sbjct: 316 DGSCRTPIAGYASKDEEGNCIFRGLVASPDGTKVLETSRKGPYVYEDMVKMGKDAGQELL 375

Query: 369 SRAGPGFF 375
           SRAGPGFF
Sbjct: 376 SRAGPGFF 380

[Close]

BLAST of MS016069 vs. ExPASy Swiss-Prot
Match: Q6H6D2 (Porphobilinogen deaminase, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=HEMC PE=2 SV=1)

HSP 1 Score: 494.6 bits (1272), Expect = 1.0e-138
Identity = 252/326 (77.30%), Postives = 285/326 (87.42%), Query Frame = 0


Query: 51  VRASVAVEQQTEKTKVALVRIGTRGSPLALAQAYETRDKLMAIHPELAEDGAIQIIIIKT 110
           VRA+VAV+ + +  KV+L+RIGTRGSPLALAQA+ETRDKL A H ELAE+GA++I+IIKT
Sbjct: 33  VRAAVAVQAEAQ-AKVSLIRIGTRGSPLALAQAHETRDKLKAAHSELAEEGAVEIVIIKT 92

Query: 111 TGDKILSQPLADIGGKGLFTKEIDDALINSDIDIAVHSMKDVPTYLPEKTILPCNLPRED 170
           TGD IL +PLADIGGKGLFTKEIDDAL+   IDIAVHSMKDVPTYLPE TILPCNLPRED
Sbjct: 93  TGDMILDKPLADIGGKGLFTKEIDDALLQGRIDIAVHSMKDVPTYLPEGTILPCNLPRED 152

Query: 171 VRDAFISLSAGSLAELPAGSVIGTASLRRKSQLLNRYPSLKVLENFRGNVQTRLRKLNEG 230
           VRDAFI L+A SLAELPAGSV+G+ASLRR+SQ+L +YPSLKV+ NFRGNVQTRLRKL EG
Sbjct: 153 VRDAFICLTASSLAELPAGSVVGSASLRRQSQILYKYPSLKVV-NFRGNVQTRLRKLKEG 212

Query: 231 VVQATLLALAGLRRLNMTENVTSVLSIDEMLPAVAQGAIGIACRSNDDKMANYLASLNHE 290
            V ATLLALAGL+RLNM E  TSVLS+DEMLPAVAQGAIGIACRS+DD M NYL+SLNHE
Sbjct: 213 DVHATLLALAGLKRLNMAETATSVLSVDEMLPAVAQGAIGIACRSSDDTMMNYLSSLNHE 272

Query: 291 ETRLAVVCERAFLETLDGSCRTPIAGYASKDEDGNCIFKGLVASPDGTRVLETSRRGAYA 350
           +TRLAV CER FL  LDG+CRTPIA YAS+D+DGNC F+GL+ASPDG+ V ETSR G Y 
Sbjct: 273 DTRLAVACEREFLSVLDGNCRTPIAAYASRDKDGNCSFRGLLASPDGSTVYETSRTGPYD 332

Query: 351 IDDMVAMGKDAGKELLSRAGPGFFDS 377
            D MV MGKDAG EL ++AGPGFFDS
Sbjct: 333 FDIMVEMGKDAGHELKAKAGPGFFDS 356

[Close]

BLAST of MS016069 vs. ExPASy Swiss-Prot
Match: A1B853 (Porphobilinogen deaminase OS=Paracoccus denitrificans (strain Pd 1222) OX=318586 GN=hemC PE=3 SV=1)

HSP 1 Score: 312.4 bits (799), Expect = 7.0e-84
Identity = 170/307 (55.37%), Postives = 219/307 (71.34%), Query Frame = 0


Query: 69  VRIGTRGSPLALAQAYETRDKLMAIHPELAEDGAIQIIIIKTTGDKILSQPLADIGGKGL 128
           +RIGTRGS LALAQA+ETRD+LMA H  LA D A +I++IKTTGD++L +PL +IGGKGL
Sbjct: 11  IRIGTRGSALALAQAHETRDRLMAAH-GLAAD-AFRIVVIKTTGDRVLDRPLKEIGGKGL 70

Query: 129 FTKEIDDALINSDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSAGSLAELPA 188
           FT+EI+DAL+  +IDIAVHSMKD+PT  PE  ++ C LPREDVRDAF+S    +++ELP 
Sbjct: 71  FTREIEDALLAHEIDIAVHSMKDMPTIQPEGLVIDCYLPREDVRDAFVSAQFAAISELPQ 130

Query: 189 GSVIGTASLRRKSQLLNRYPSLKVLENFRGNVQTRLRKLNEGVVQATLLALAGLRRLNMT 248
           G+V+G++SLRR++QL  R P LK++E FRGNVQTRL+KL +GV  AT LA+AGL RL M 
Sbjct: 131 GAVVGSSSLRRRAQLAARRPDLKLVE-FRGNVQTRLKKLEDGVAVATFLAMAGLTRLGML 190

Query: 249 ENVTSVLSIDEMLPAVAQGAIGIACRSNDDKMANYLASLNHEETRLAVVCERAFLETLDG 308
                 +  DEMLPAVAQG IG+  R++D + A+ LA+++  ++ L V  ERAFL  LDG
Sbjct: 191 HVARGAVEPDEMLPAVAQGCIGVERRADDARTASLLAAISDRDSALRVTAERAFLARLDG 250

Query: 309 SCRTPIAGYASKDEDGNCIFKGLVASPDGTRVLETSRRGAYAIDDMVAMGKDAGKELLSR 368
           SC+TPIAG A    D     +G +  PDG+ V+   R G  A  D  AMG D  +EL  R
Sbjct: 251 SCQTPIAGLAELQGD-RLRLRGEILRPDGSEVIAAERVGPAA--DGAAMGTDLAEELRGR 310

Query: 369 AGPGFFD 376
           A   FFD
Sbjct: 311 APADFFD 311

[Close]

BLAST of MS016069 vs. ExPASy Swiss-Prot
Match: Q3SVF3 (Porphobilinogen deaminase OS=Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255) OX=323098 GN=hemC PE=3 SV=1)

HSP 1 Score: 310.5 bits (794), Expect = 2.7e-83
Identity = 172/316 (54.43%), Postives = 216/316 (68.35%), Query Frame = 0


Query: 59  QQTEKTKVALVRIGTRGSPLALAQAYETRDKLMAIHPELAEDGAIQIIIIKTTGDKILSQ 118
           Q +++T + L  IGTRGSPLALAQA+E RD+L   H    E   I I  I+T+GD I  +
Sbjct: 2   QSSDETDI-LATIGTRGSPLALAQAHEVRDRLARAHQVAPE--RIAIKTIRTSGDAIQDR 61

Query: 119 PLADIGGKGLFTKEIDDALINSDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISL 178
           PL D+GGKGLFTKEI++AL+   ID AVHS KDVPT+LP+ T LP  LPREDVRD FIS 
Sbjct: 62  PLFDVGGKGLFTKEIEEALLAGTIDFAVHSSKDVPTFLPDATWLPAFLPREDVRDVFISP 121

Query: 179 SAGSLAELPAGSVIGTASLRRKSQLLNRYPSLKVLENFRGNVQTRLRKLNEGVVQATLLA 238
            AGSL +LPAG+ +GTASLRR++ +L   P LKV  + RGNV+TRLRK++ G   ATLLA
Sbjct: 122 HAGSLNDLPAGATVGTASLRRQAMVLKLRPDLKV-NSLRGNVETRLRKISVGEADATLLA 181

Query: 239 LAGLRRLNMTENVTSVLSIDEMLPAVAQGAIGIACRSNDDKMANYLASLNHEETRLAVVC 298
           LAGL RL + +  T +L  DE LPAV QGAI I  R +DD++  ++ ++   ET +A+  
Sbjct: 182 LAGLNRLGLQDKATRILETDEFLPAVGQGAIAIESRRDDDRINAFVKAIGDPETEVALSA 241

Query: 299 ERAFLETLDGSCRTPIAGYASKDEDGNCIFKGLVASPDGTRVLETSRRGAYAIDDMVAMG 358
           ER+FL  LDGSCRTPI G+   + D    F+GL+ SPDGT   ET+R GA A  D  A+G
Sbjct: 242 ERSFLALLDGSCRTPIGGHCRVNGD-RIDFRGLIISPDGTEFYETTREGARA--DAAALG 301

Query: 359 KDAGKELLSRAGPGFF 375
            DA  EL  RAG  FF
Sbjct: 302 ADAAHELRERAGEKFF 310

[Close]

BLAST of MS016069 vs. ExPASy TrEMBL
Match: A0A6J1DII9 (Hydroxymethylbilane synthase OS=Momordica charantia OX=3673 GN=LOC111020357 PE=3 SV=1)

HSP 1 Score: 728.0 bits (1878), Expect = 2.0e-206
Identity = 374/376 (99.47%), Postives = 374/376 (99.47%), Query Frame = 0


Query: 1   MGVFSSPPITHSPMARPCNVGSLSLLGFSSLSLKPPAFCNGGKKFGGLGLVRASVAVEQQ 60
           MGVFSSPPITHSPMARPCNVGSLS LGFSSLSLKPPAFCNGGKKFGGLGLVRASVAVEQQ
Sbjct: 1   MGVFSSPPITHSPMARPCNVGSLSPLGFSSLSLKPPAFCNGGKKFGGLGLVRASVAVEQQ 60

Query: 61  TEKTKVALVRIGTRGSPLALAQAYETRDKLMAIHPELAEDGAIQIIIIKTTGDKILSQPL 120
           TEKTKVALVRIGTRGSPLALAQAYETRDKLMAIHPELAEDGAIQIIIIKTTGDKILSQPL
Sbjct: 61  TEKTKVALVRIGTRGSPLALAQAYETRDKLMAIHPELAEDGAIQIIIIKTTGDKILSQPL 120

Query: 121 ADIGGKGLFTKEIDDALINSDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSA 180
           ADIGGKGLFTKEIDDALINSDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSA
Sbjct: 121 ADIGGKGLFTKEIDDALINSDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSA 180

Query: 181 GSLAELPAGSVIGTASLRRKSQLLNRYPSLKVLENFRGNVQTRLRKLNEGVVQATLLALA 240
           GSLAELPAGSVIGTASLRRKSQLLNRYPSLKVLENFRGNVQTRLRKLNEGVVQATLLALA
Sbjct: 181 GSLAELPAGSVIGTASLRRKSQLLNRYPSLKVLENFRGNVQTRLRKLNEGVVQATLLALA 240

Query: 241 GLRRLNMTENVTSVLSIDEMLPAVAQGAIGIACRSNDDKMANYLASLNHEETRLAVVCER 300
           GLRRLNMTENVTSVLSIDEMLPAVAQGAIGIACRSNDDKMANYLASLNHEETRLAVVCER
Sbjct: 241 GLRRLNMTENVTSVLSIDEMLPAVAQGAIGIACRSNDDKMANYLASLNHEETRLAVVCER 300

Query: 301 AFLETLDGSCRTPIAGYASKDEDGNCIFKGLVASPDGTRVLETSRRGAYAIDDMVAMGKD 360
           AFLE LDGSCRTPIAGYASKDEDGNCIFKGLVASPDGTRVLETSRRGAYAIDDMVAMGKD
Sbjct: 301 AFLEMLDGSCRTPIAGYASKDEDGNCIFKGLVASPDGTRVLETSRRGAYAIDDMVAMGKD 360

Query: 361 AGKELLSRAGPGFFDS 377
           AGKELLSRAGPGFFDS
Sbjct: 361 AGKELLSRAGPGFFDS 376

[Close]

BLAST of MS016069 vs. ExPASy TrEMBL
Match: A0A6J1GCC7 (Hydroxymethylbilane synthase OS=Cucurbita moschata OX=3662 GN=LOC111452689 PE=3 SV=1)

HSP 1 Score: 689.9 bits (1779), Expect = 6.0e-195
Identity = 352/376 (93.62%), Postives = 362/376 (96.28%), Query Frame = 0


Query: 1   MGVFSSPPITHSPMARPCNVGSLSLLGFSSLSLKPPAFCNGGKKFGGLGLVRASVAVEQQ 60
           MGVFSSP I+H PMARPCN+GSLS  GFSSLSLKPPAF  GGKKF G GL+RASVAVEQQ
Sbjct: 1   MGVFSSPSISHYPMARPCNLGSLSRHGFSSLSLKPPAFSTGGKKFHGFGLIRASVAVEQQ 60

Query: 61  TEKTKVALVRIGTRGSPLALAQAYETRDKLMAIHPELAEDGAIQIIIIKTTGDKILSQPL 120
            EKTKVALVRIGTRGSPLALAQA+ETR+KLMAIHPELAEDGAIQI+IIKTTGDKILSQPL
Sbjct: 61  AEKTKVALVRIGTRGSPLALAQAHETREKLMAIHPELAEDGAIQIVIIKTTGDKILSQPL 120

Query: 121 ADIGGKGLFTKEIDDALINSDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSA 180
           ADIGGKGLFTKEIDDALIN DIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLS 
Sbjct: 121 ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSV 180

Query: 181 GSLAELPAGSVIGTASLRRKSQLLNRYPSLKVLENFRGNVQTRLRKLNEGVVQATLLALA 240
           GSLAELPAGSVIGTASLRRKSQLLNRYPSL VLENFRGNVQTRLRKLNEGVVQATLLALA
Sbjct: 181 GSLAELPAGSVIGTASLRRKSQLLNRYPSLNVLENFRGNVQTRLRKLNEGVVQATLLALA 240

Query: 241 GLRRLNMTENVTSVLSIDEMLPAVAQGAIGIACRSNDDKMANYLASLNHEETRLAVVCER 300
           GLRRLNMTE VTS+LSIDEMLPAVAQGAIGIACRS+DDKMANYLASLNHEETRLAVVCER
Sbjct: 241 GLRRLNMTETVTSILSIDEMLPAVAQGAIGIACRSDDDKMANYLASLNHEETRLAVVCER 300

Query: 301 AFLETLDGSCRTPIAGYASKDEDGNCIFKGLVASPDGTRVLETSRRGAYAIDDMVAMGKD 360
           AFLETLDGSCRTPIAGYAS+DEDGNCIFKGLVASPDGTRVLETSRRG YAIDDM+AMGKD
Sbjct: 301 AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAIDDMIAMGKD 360

Query: 361 AGKELLSRAGPGFFDS 377
           AGKELLSRAGPGFFDS
Sbjct: 361 AGKELLSRAGPGFFDS 376

[Close]

BLAST of MS016069 vs. ExPASy TrEMBL
Match: A0A6J1KA97 (Hydroxymethylbilane synthase OS=Cucurbita maxima OX=3661 GN=LOC111493083 PE=3 SV=1)

HSP 1 Score: 686.8 bits (1771), Expect = 5.1e-194
Identity = 351/376 (93.35%), Postives = 362/376 (96.28%), Query Frame = 0


Query: 1   MGVFSSPPITHSPMARPCNVGSLSLLGFSSLSLKPPAFCNGGKKFGGLGLVRASVAVEQQ 60
           MGVFSSP I+H PMARPCN+GSLS  GFSSLSLKPPAF  G K+F G+GL+RASVAVEQQ
Sbjct: 1   MGVFSSPSISHYPMARPCNLGSLSRHGFSSLSLKPPAFSTGDKRFHGVGLIRASVAVEQQ 60

Query: 61  TEKTKVALVRIGTRGSPLALAQAYETRDKLMAIHPELAEDGAIQIIIIKTTGDKILSQPL 120
            EKTKVALVRIGTRGSPLALAQA+ETR+KLMAIHPELAEDGAIQI+IIKTTGDKILSQPL
Sbjct: 61  AEKTKVALVRIGTRGSPLALAQAHETREKLMAIHPELAEDGAIQIVIIKTTGDKILSQPL 120

Query: 121 ADIGGKGLFTKEIDDALINSDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSA 180
           ADIGGKGLFTKEIDDALIN DIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLS 
Sbjct: 121 ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSV 180

Query: 181 GSLAELPAGSVIGTASLRRKSQLLNRYPSLKVLENFRGNVQTRLRKLNEGVVQATLLALA 240
           GSLAELPAGSVIGTASLRRKSQLLNRYPSL VLENFRGNVQTRLRKLNEGVVQATLLALA
Sbjct: 181 GSLAELPAGSVIGTASLRRKSQLLNRYPSLNVLENFRGNVQTRLRKLNEGVVQATLLALA 240

Query: 241 GLRRLNMTENVTSVLSIDEMLPAVAQGAIGIACRSNDDKMANYLASLNHEETRLAVVCER 300
           GLRRLNMTE VTS+LSIDEMLPAVAQGAIGIACRS+DDKMANYLASLNHEETRLAVVCER
Sbjct: 241 GLRRLNMTETVTSILSIDEMLPAVAQGAIGIACRSDDDKMANYLASLNHEETRLAVVCER 300

Query: 301 AFLETLDGSCRTPIAGYASKDEDGNCIFKGLVASPDGTRVLETSRRGAYAIDDMVAMGKD 360
           AFLETLDGSCRTPIAGYAS+DEDGNCIFKGLVASPDGTRVLETSRRG YAIDDMVAMGKD
Sbjct: 301 AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAIDDMVAMGKD 360

Query: 361 AGKELLSRAGPGFFDS 377
           AGKELLSRAGPGFFDS
Sbjct: 361 AGKELLSRAGPGFFDS 376

[Close]

BLAST of MS016069 vs. ExPASy TrEMBL
Match: A0A6J1IZW2 (Hydroxymethylbilane synthase OS=Cucurbita maxima OX=3661 GN=LOC111481450 PE=3 SV=1)

HSP 1 Score: 677.6 bits (1747), Expect = 3.1e-191
Identity = 344/376 (91.49%), Postives = 361/376 (96.01%), Query Frame = 0


Query: 1   MGVFSSPPITHSPMARPCNVGSLSLLGFSSLSLKPPAFCNGGKKFGGLGLVRASVAVEQQ 60
           M   SSP +++SPMARPCN+ SLSL GFSSLSLKPPAF N  KKF G GL+RASVAVEQQ
Sbjct: 1   MVALSSPSVSNSPMARPCNLASLSLPGFSSLSLKPPAFTNAPKKFHGFGLIRASVAVEQQ 60

Query: 61  TEKTKVALVRIGTRGSPLALAQAYETRDKLMAIHPELAEDGAIQIIIIKTTGDKILSQPL 120
            EKTKVAL+RIGTRGSPLALAQA+ETRDKLMA HPELAEDGAIQI++IKTTGDKILSQPL
Sbjct: 61  PEKTKVALLRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPL 120

Query: 121 ADIGGKGLFTKEIDDALINSDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSA 180
           ADIGGKGLFTKEIDDALIN DIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISL A
Sbjct: 121 ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLRA 180

Query: 181 GSLAELPAGSVIGTASLRRKSQLLNRYPSLKVLENFRGNVQTRLRKLNEGVVQATLLALA 240
           GSLAELPAGSV+GTASLRRKSQLLNRYPSL+VLENFRGNVQTRL+KLNEGVVQATLLALA
Sbjct: 181 GSLAELPAGSVVGTASLRRKSQLLNRYPSLEVLENFRGNVQTRLKKLNEGVVQATLLALA 240

Query: 241 GLRRLNMTENVTSVLSIDEMLPAVAQGAIGIACRSNDDKMANYLASLNHEETRLAVVCER 300
           GLRRLNMTENVTS+LSIDEMLPAVAQGAIGIACRS+DDKMANYLASLNHEETRLAVVCER
Sbjct: 241 GLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDKMANYLASLNHEETRLAVVCER 300

Query: 301 AFLETLDGSCRTPIAGYASKDEDGNCIFKGLVASPDGTRVLETSRRGAYAIDDMVAMGKD 360
           AFLETLDGSCRTPIAGYAS+DEDGNCIFKGLVASPDGTRVLETSRRG+YAIDDM+AMGKD
Sbjct: 301 AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGSYAIDDMIAMGKD 360

Query: 361 AGKELLSRAGPGFFDS 377
           AGKELLSRAGPGFFDS
Sbjct: 361 AGKELLSRAGPGFFDS 376

[Close]

BLAST of MS016069 vs. ExPASy TrEMBL
Match: A0A6J1F354 (Hydroxymethylbilane synthase OS=Cucurbita moschata OX=3662 GN=LOC111441758 PE=3 SV=1)

HSP 1 Score: 673.7 bits (1737), Expect = 4.4e-190
Identity = 342/376 (90.96%), Postives = 358/376 (95.21%), Query Frame = 0


Query: 1   MGVFSSPPITHSPMARPCNVGSLSLLGFSSLSLKPPAFCNGGKKFGGLGLVRASVAVEQQ 60
           M   SSP ++HSPMA PCN+ SLSL GFSSLSLKPPAF NG KK  G GL+RASVAVEQQ
Sbjct: 1   MVALSSPSVSHSPMAHPCNLASLSLPGFSSLSLKPPAFTNGPKKLHGFGLIRASVAVEQQ 60

Query: 61  TEKTKVALVRIGTRGSPLALAQAYETRDKLMAIHPELAEDGAIQIIIIKTTGDKILSQPL 120
             KTKVAL+RIGTRGSPLALAQA+ETRDKLMA HPELAEDGAIQI++IKTTGDKILSQPL
Sbjct: 61  AGKTKVALLRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPL 120

Query: 121 ADIGGKGLFTKEIDDALINSDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSA 180
           ADIGGKGLFTKEIDDALIN DIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISL A
Sbjct: 121 ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLRA 180

Query: 181 GSLAELPAGSVIGTASLRRKSQLLNRYPSLKVLENFRGNVQTRLRKLNEGVVQATLLALA 240
           GSLAELPAGSV+GTASLRRKSQLLNRYPSL+VLENFRGNVQTRL+KLNEGVVQATLLALA
Sbjct: 181 GSLAELPAGSVVGTASLRRKSQLLNRYPSLEVLENFRGNVQTRLKKLNEGVVQATLLALA 240

Query: 241 GLRRLNMTENVTSVLSIDEMLPAVAQGAIGIACRSNDDKMANYLASLNHEETRLAVVCER 300
           GLRRLNMTENVTS+LSIDEMLPAVAQGAIGIACRS+DDKMA YLASLNHEETRLAVVCER
Sbjct: 241 GLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDKMAEYLASLNHEETRLAVVCER 300

Query: 301 AFLETLDGSCRTPIAGYASKDEDGNCIFKGLVASPDGTRVLETSRRGAYAIDDMVAMGKD 360
           AFLETLDGSCRTPIAGYAS+DEDGNCIFKGLVASPDGTRVLETSRRG+YAIDDM+AMGKD
Sbjct: 301 AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGSYAIDDMIAMGKD 360

Query: 361 AGKELLSRAGPGFFDS 377
           AGKELLSRAGPGFFDS
Sbjct: 361 AGKELLSRAGPGFFDS 376

[Close]

BLAST of MS016069 vs. TAIR 10
Match: AT5G08280.1 (hydroxymethylbilane synthase )

HSP 1 Score: 552.7 bits (1423), Expect = 2.2e-157
Identity = 284/368 (77.17%), Postives = 318/368 (86.41%), Query Frame = 0


Query: 9   ITHSPMAR--PCNVGSLSLLGFSSLSLKPPAFCNGGKKFGGLGLVRASVAVEQQTEKTKV 68
           +T  P +R   C++GS+S +GFS   +  PA     +K    G V+A VAVEQ   KT+ 
Sbjct: 16  LTRQPSSRVNTCSLGSVSAIGFSLPQISSPALGKCRRKQSSSGFVKACVAVEQ---KTRT 75

Query: 69  ALVRIGTRGSPLALAQAYETRDKLMAIHPELAEDGAIQIIIIKTTGDKILSQPLADIGGK 128
           A++RIGTRGSPLALAQAYETR+KL   HPEL EDGAI I IIKTTGDKILSQPLADIGGK
Sbjct: 76  AIIRIGTRGSPLALAQAYETREKLKKKHPELVEDGAIHIEIIKTTGDKILSQPLADIGGK 135

Query: 129 GLFTKEIDDALINSDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSAGSLAEL 188
           GLFTKEID+ALIN  IDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFI L+A +LAEL
Sbjct: 136 GLFTKEIDEALINGHIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFICLTAATLAEL 195

Query: 189 PAGSVIGTASLRRKSQLLNRYPSLKVLENFRGNVQTRLRKLNEGVVQATLLALAGLRRLN 248
           PAGSV+GTASLRRKSQ+L++YP+L V ENFRGNVQTRL KL  G VQATLLALAGL+RL+
Sbjct: 196 PAGSVVGTASLRRKSQILHKYPALHVEENFRGNVQTRLSKLQGGKVQATLLALAGLKRLS 255

Query: 249 MTENVTSVLSIDEMLPAVAQGAIGIACRSNDDKMANYLASLNHEETRLAVVCERAFLETL 308
           MTENV S+LS+DEMLPAVAQGAIGIACR++DDKMA YLASLNHEETRLA+ CERAFLETL
Sbjct: 256 MTENVASILSLDEMLPAVAQGAIGIACRTDDDKMATYLASLNHEETRLAISCERAFLETL 315

Query: 309 DGSCRTPIAGYASKDEDGNCIFKGLVASPDGTRVLETSRRGAYAIDDMVAMGKDAGKELL 368
           DGSCRTPIAGYASKDE+GNCIF+GLVASPDGT+VLETSR+G Y  +DMV MGKDAG+ELL
Sbjct: 316 DGSCRTPIAGYASKDEEGNCIFRGLVASPDGTKVLETSRKGPYVYEDMVKMGKDAGQELL 375

Query: 369 SRAGPGFF 375
           SRAGPGFF
Sbjct: 376 SRAGPGFF 380

The following BLAST results are available for this feature:

BLAST of MS016069 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022152701.1	4.1e-206	99.47	porphobilinogen deaminase, chloroplastic [Momordica charantia]	[more]
XP_038906286.1	6.6e-196	93.88	porphobilinogen deaminase, chloroplastic [Benincasa hispida]	[more]
XP_022949290.1	1.2e-194	93.62	porphobilinogen deaminase, chloroplastic-like [Cucurbita moschata]	[more]
KAG7036601.1	2.8e-194	93.35	Porphobilinogen deaminase, chloroplastic [Cucurbita argyrosperma subsp. argyrosp...	[more]
XP_023523547.1	6.1e-194	93.35	porphobilinogen deaminase, chloroplastic-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of MS016069 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q43082	8.7e-159	81.95	Porphobilinogen deaminase, chloroplastic OS=Pisum sativum OX=3888 GN=HEMC PE=1 S...	[more]
Q43316	3.1e-156	77.17	Porphobilinogen deaminase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=HEMC...	[more]
Q6H6D2	1.0e-138	77.30	Porphobilinogen deaminase, chloroplastic OS=Oryza sativa subsp. japonica OX=3994...	[more]
A1B853	7.0e-84	55.37	Porphobilinogen deaminase OS=Paracoccus denitrificans (strain Pd 1222) OX=318586...	[more]
Q3SVF3	2.7e-83	54.43	Porphobilinogen deaminase OS=Nitrobacter winogradskyi (strain ATCC 25391 / DSM 1...	[more]

BLAST of MS016069 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DII9	2.0e-206	99.47	Hydroxymethylbilane synthase OS=Momordica charantia OX=3673 GN=LOC111020357 PE=3...	[more]
A0A6J1GCC7	6.0e-195	93.62	Hydroxymethylbilane synthase OS=Cucurbita moschata OX=3662 GN=LOC111452689 PE=3 ...	[more]
A0A6J1KA97	5.1e-194	93.35	Hydroxymethylbilane synthase OS=Cucurbita maxima OX=3661 GN=LOC111493083 PE=3 SV...	[more]
A0A6J1IZW2	3.1e-191	91.49	Hydroxymethylbilane synthase OS=Cucurbita maxima OX=3661 GN=LOC111481450 PE=3 SV...	[more]
A0A6J1F354	4.4e-190	90.96	Hydroxymethylbilane synthase OS=Cucurbita moschata OX=3662 GN=LOC111441758 PE=3 ...	[more]

BLAST of MS016069 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G08280.1	2.2e-157	77.17	hydroxymethylbilane synthase	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000860	Porphobilinogen deaminase	PRINTS	PR00151	PORPHBDMNASE	coord: 191..208 score: 56.08 coord: 112..132 score: 66.21 coord: 143..162 score: 61.19 coord: 298..315 score: 53.17 coord: 211..228 score: 53.97
IPR000860	Porphobilinogen deaminase	TIGRFAM	TIGR00212	TIGR00212	coord: 69..368 e-value: 1.3E-100 score: 334.2
IPR000860	Porphobilinogen deaminase	PIRSF	PIRSF001438	PBGD	coord: 51..376 e-value: 1.8E-116 score: 386.5
IPR000860	Porphobilinogen deaminase	PANTHER	PTHR11557	PORPHOBILINOGEN DEAMINASE	coord: 15..375
IPR000860	Porphobilinogen deaminase	HAMAP	MF_00260	Porphobil_deam	coord: 68..368 score: 34.057735
None	No IPR available	GENE3D	3.40.190.10		coord: 69..278 e-value: 1.5E-80 score: 271.3
None	No IPR available	GENE3D	3.40.190.10		coord: 170..270 e-value: 1.5E-80 score: 271.3
None	No IPR available	PANTHER	PTHR11557:SF8	BNAC02G01240D PROTEIN	coord: 15..375
None	No IPR available	CDD	cd13648	PBP2_PBGD_1	coord: 68..347 e-value: 0.0 score: 514.269
None	No IPR available	SUPERFAMILY	53850	Periplasmic binding protein-like II	coord: 69..286
IPR022417	Porphobilinogen deaminase, N-terminal	PFAM	PF01379	Porphobil_deam	coord: 69..281 e-value: 1.6E-75 score: 253.2
IPR036803	Porphobilinogen deaminase, C-terminal domain superfamily	GENE3D	3.30.160.40		coord: 289..375 e-value: 7.0E-24 score: 86.0
IPR036803	Porphobilinogen deaminase, C-terminal domain superfamily	SUPERFAMILY	54782	Porphobilinogen deaminase (hydroxymethylbilane synthase), C-terminal domain	coord: 288..375
IPR022418	Porphobilinogen deaminase, C-terminal	PFAM	PF03900	Porphobil_deamC	coord: 294..366 e-value: 3.2E-15 score: 56.3
IPR022419	Porphobilinogen deaminase, dipyrromethane cofactor binding site	PROSITE	PS00533	PORPHOBILINOGEN_DEAM	coord: 299..315

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS016069.1	MS016069.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0018160	peptidyl-pyrromethane cofactor linkage
biological_process	GO:0033014	tetrapyrrole biosynthetic process
molecular_function	GO:0004418	hydroxymethylbilane synthase activity