MS016062 (gene) Bitter gourd (TR) v1

BLAST of MS016062 vs. NCBI nr
Match: XP_022144395.1 (L-ascorbate oxidase homolog [Momordica charantia])

HSP 1 Score: 1121.7 bits (2900), Expect = 0.0e+00
Identity = 539/541 (99.63%), Postives = 539/541 (99.63%), Query Frame = 0

Query: 1   MAIASAPLLCLCVSFFAIVAADDPYRFFNWNVTYGTIYPLGLPQQGILINGQFPGPDIHS 60
           MAIASAPLLCLCVSFFAIVAADDPYRFFNWNVTYGTIYPLGLPQQGILINGQFPGPDIHS
Sbjct: 1   MAIASAPLLCLCVSFFAIVAADDPYRFFNWNVTYGTIYPLGLPQQGILINGQFPGPDIHS 60

Query: 61  VTNDNLIINVFNSLDEPFLLSWNGIQQRRNSYEDGVYGTTCPIPPGKNFTYILQVKDQIG 120
           VTNDNLIINVFNSLDEPFLLSWNGIQQRRNSYEDGVYGTTCPIPPGKNFTYILQVKDQIG
Sbjct: 61  VTNDNLIINVFNSLDEPFLLSWNGIQQRRNSYEDGVYGTTCPIPPGKNFTYILQVKDQIG 120

Query: 121 SFYYFPSLSFHKAAGGFGGIRILSRPRIPVPFPDPAGDYTVLIGDWYQANHTTLRAQLDN 180
           SFYYFPSL FHKAAGGFGGIRILSRPRIPVPFPDPAGDYTVLIGDWYQANHTTLRAQLDN
Sbjct: 121 SFYYFPSLGFHKAAGGFGGIRILSRPRIPVPFPDPAGDYTVLIGDWYQANHTTLRAQLDN 180

Query: 181 GTMLPLPDGILINGRGPNRTAPFNVEQGKTYRLRISNVGLQNSLNFRIQGHRLKVVEVEG 240
           GTMLPLPDGILINGRGPNRTAPFNVEQGKTYRLRISNVGLQNSLNFRIQGHRLKVVEVEG
Sbjct: 181 GTMLPLPDGILINGRGPNRTAPFNVEQGKTYRLRISNVGLQNSLNFRIQGHRLKVVEVEG 240

Query: 241 THTLQTEFSSLDIHVGQSYSVLVTADQPAQDFYVVVSSRFTTPILTTTAILRYANSAGSV 300
           THTLQTEFSSLDIHVGQ YSVLVTADQPAQDFYVVVSSRFTTPILTTTAILRYANSAGSV
Sbjct: 241 THTLQTEFSSLDIHVGQFYSVLVTADQPAQDFYVVVSSRFTTPILTTTAILRYANSAGSV 300

Query: 301 QGPPPGGPTIQIDWSLNQARAIRTNLTASGPRPNPQGSYHYGLINTTKTIVLSNSAGFVN 360
           QGPPPGGPTIQIDWSLNQARAIRTNLTASGPRPNPQGSYHYGLINTTKTIVLSNSAGFVN
Sbjct: 301 QGPPPGGPTIQIDWSLNQARAIRTNLTASGPRPNPQGSYHYGLINTTKTIVLSNSAGFVN 360

Query: 361 GKQRYAVNSVSFIPADTPLKVADFFKIGGVFRVGSISDWPTGGGIYQDTSVMGADYRAYV 420
           GKQRYAVNSVSFIPADTPLKVADFFKIGGVFRVGSISDWPTGGGIYQDTSVMGADYRAYV
Sbjct: 361 GKQRYAVNSVSFIPADTPLKVADFFKIGGVFRVGSISDWPTGGGIYQDTSVMGADYRAYV 420

Query: 421 EIVFQNNEDLIQSWHLDGYSFFVVGMDGGQWTSNSRNQYNLRDAIARCTTQVYPKSWTAI 480
           EIVFQNNEDLIQSWHLDGYSFFVVGMDGGQWTSNSRNQYNLRDAIARCTTQVYPKSWTAI
Sbjct: 421 EIVFQNNEDLIQSWHLDGYSFFVVGMDGGQWTSNSRNQYNLRDAIARCTTQVYPKSWTAI 480

Query: 481 YVALDNVGMWNVRSEFWARQYLGQQFYLRVYTPSTSLRDEYPIPKNALLCGRATGRRTRP 540
           YVALDNVGMWNVRSEFWARQYLGQQFYLRVYTPSTSLRDEYPIPKNALLCGRATGRRTRP
Sbjct: 481 YVALDNVGMWNVRSEFWARQYLGQQFYLRVYTPSTSLRDEYPIPKNALLCGRATGRRTRP 540

Query: 541 L 542
           L
Sbjct: 541 L 541

BLAST of MS016062 vs. NCBI nr
Match: XP_023547180.1 (L-ascorbate oxidase homolog [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1094.0 bits (2828), Expect = 0.0e+00
Identity = 523/546 (95.79%), Postives = 535/546 (97.99%), Query Frame = 0

Query: 1   MAIAS-----APLLCLCVSFFAIVAADDPYRFFNWNVTYGTIYPLGLPQQGILINGQFPG 60
           MAI+S      PLLCLC+SFFAIV ADDPYRFFNWNVTYGTIYPLGLPQQGILINGQFPG
Sbjct: 1   MAISSMRSSLPPLLCLCISFFAIVGADDPYRFFNWNVTYGTIYPLGLPQQGILINGQFPG 60

Query: 61  PDIHSVTNDNLIINVFNSLDEPFLLSWNGIQQRRNSYEDGVYGTTCPIPPGKNFTYILQV 120
           PDIHSVTNDN+IINV+NSLDEPFLLSWNGIQQRRNSYEDGVYGTTCPIPPGKNFTYILQ+
Sbjct: 61  PDIHSVTNDNIIINVYNSLDEPFLLSWNGIQQRRNSYEDGVYGTTCPIPPGKNFTYILQM 120

Query: 121 KDQIGSFYYFPSLSFHKAAGGFGGIRILSRPRIPVPFPDPAGDYTVLIGDWYQANHTTLR 180
           KDQIGSFYYFPSL FHKAAGGFGGIRILSRPRIPVPFPDPAGDYTVLIGDWYQANHTTLR
Sbjct: 121 KDQIGSFYYFPSLGFHKAAGGFGGIRILSRPRIPVPFPDPAGDYTVLIGDWYQANHTTLR 180

Query: 181 AQLDNGTMLPLPDGILINGRGPNRTAPFNVEQGKTYRLRISNVGLQNSLNFRIQGHRLKV 240
           AQLDNG+MLPLPDGILINGRGPNRTAPFNVEQGKTYRLRISNVGLQ+SLNFRIQGHRLKV
Sbjct: 181 AQLDNGSMLPLPDGILINGRGPNRTAPFNVEQGKTYRLRISNVGLQSSLNFRIQGHRLKV 240

Query: 241 VEVEGTHTLQTEFSSLDIHVGQSYSVLVTADQPAQDFYVVVSSRFTTPILTTTAILRYAN 300
           VEVEGTH+LQTEFSSLDIHVGQSYSVLVTADQPAQDFY+VVSSRFTTPILTTTAILRYAN
Sbjct: 241 VEVEGTHSLQTEFSSLDIHVGQSYSVLVTADQPAQDFYIVVSSRFTTPILTTTAILRYAN 300

Query: 301 SAGSVQGPPPGGPTIQIDWSLNQARAIRTNLTASGPRPNPQGSYHYGLINTTKTIVLSNS 360
           SAGSVQGPPPGGPTIQIDWSLNQARAIRTNLTASGPRPNPQGSYHYGLINTTKTIVLSNS
Sbjct: 301 SAGSVQGPPPGGPTIQIDWSLNQARAIRTNLTASGPRPNPQGSYHYGLINTTKTIVLSNS 360

Query: 361 AGFVNGKQRYAVNSVSFIPADTPLKVADFFKIGGVFRVGSISDWPTGGGIYQDTSVMGAD 420
           AG VNGKQRYAVNSVSFIPADTPLKVADFFKIGGVFRVGSISDWP G GIY+DTSVMGAD
Sbjct: 361 AGLVNGKQRYAVNSVSFIPADTPLKVADFFKIGGVFRVGSISDWPNGAGIYEDTSVMGAD 420

Query: 421 YRAYVEIVFQNNEDLIQSWHLDGYSFFVVGMDGGQWTSNSRNQYNLRDAIARCTTQVYPK 480
           YRA+VEIVFQNNEDLIQSWHLDGYSFFVVGMDGGQWTSNSRNQYNLRDAIARCTTQVYPK
Sbjct: 421 YRAFVEIVFQNNEDLIQSWHLDGYSFFVVGMDGGQWTSNSRNQYNLRDAIARCTTQVYPK 480

Query: 481 SWTAIYVALDNVGMWNVRSEFWARQYLGQQFYLRVYTPSTSLRDEYPIPKNALLCGRATG 540
           SWTAIYVALDNVGMWN+RSEFWARQYLGQQFYLRVYTPSTSLRDEYPIPKNALLCGRATG
Sbjct: 481 SWTAIYVALDNVGMWNLRSEFWARQYLGQQFYLRVYTPSTSLRDEYPIPKNALLCGRATG 540

Query: 541 RRTRPL 542
           RRTRPL
Sbjct: 541 RRTRPL 546

BLAST of MS016062 vs. NCBI nr
Match: KAG6598531.1 (L-ascorbate oxidase-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1093.6 bits (2827), Expect = 0.0e+00
Identity = 523/546 (95.79%), Postives = 535/546 (97.99%), Query Frame = 0

Query: 1   MAIAS-----APLLCLCVSFFAIVAADDPYRFFNWNVTYGTIYPLGLPQQGILINGQFPG 60
           MAI+S      PLLCLC+SFFAIV ADDPYRFFNWNVTYGTIYPLGLPQQGILINGQFPG
Sbjct: 1   MAISSMRSSLPPLLCLCISFFAIVGADDPYRFFNWNVTYGTIYPLGLPQQGILINGQFPG 60

Query: 61  PDIHSVTNDNLIINVFNSLDEPFLLSWNGIQQRRNSYEDGVYGTTCPIPPGKNFTYILQV 120
           PDIHSVTNDN+IINVFNSLDEPFLLSWNGIQQRRNSYEDGVYGTTCPIPPGKNFTYILQ+
Sbjct: 61  PDIHSVTNDNIIINVFNSLDEPFLLSWNGIQQRRNSYEDGVYGTTCPIPPGKNFTYILQM 120

Query: 121 KDQIGSFYYFPSLSFHKAAGGFGGIRILSRPRIPVPFPDPAGDYTVLIGDWYQANHTTLR 180
           KDQIGSFYYFPSL FHKAAGGFGGIRILSRPRIPVPFPDPAGDYTVLIGDWYQANHTTLR
Sbjct: 121 KDQIGSFYYFPSLGFHKAAGGFGGIRILSRPRIPVPFPDPAGDYTVLIGDWYQANHTTLR 180

Query: 181 AQLDNGTMLPLPDGILINGRGPNRTAPFNVEQGKTYRLRISNVGLQNSLNFRIQGHRLKV 240
           AQLDNG+MLPLPDGILINGRGPNRTAPFNVEQGKTYRLRISNVGLQ+SLNFRIQGHRLKV
Sbjct: 181 AQLDNGSMLPLPDGILINGRGPNRTAPFNVEQGKTYRLRISNVGLQSSLNFRIQGHRLKV 240

Query: 241 VEVEGTHTLQTEFSSLDIHVGQSYSVLVTADQPAQDFYVVVSSRFTTPILTTTAILRYAN 300
           VEVEGTH+LQTEFSSLDIHVGQSYSVLVTADQPAQDFY+VVSSRFTTPILTTTAILRYAN
Sbjct: 241 VEVEGTHSLQTEFSSLDIHVGQSYSVLVTADQPAQDFYIVVSSRFTTPILTTTAILRYAN 300

Query: 301 SAGSVQGPPPGGPTIQIDWSLNQARAIRTNLTASGPRPNPQGSYHYGLINTTKTIVLSNS 360
           SAGSVQGPPPGGPTIQIDWSLNQARAIRTNLTASGPRPNPQGSYHYGLINTTKTIVLSNS
Sbjct: 301 SAGSVQGPPPGGPTIQIDWSLNQARAIRTNLTASGPRPNPQGSYHYGLINTTKTIVLSNS 360

Query: 361 AGFVNGKQRYAVNSVSFIPADTPLKVADFFKIGGVFRVGSISDWPTGGGIYQDTSVMGAD 420
           AG VNGKQRYAVNSVSFIPADTPLKVADFFKIGGVFRVGSIS+WP G GIY+DTSVMGAD
Sbjct: 361 AGLVNGKQRYAVNSVSFIPADTPLKVADFFKIGGVFRVGSISEWPNGAGIYEDTSVMGAD 420

Query: 421 YRAYVEIVFQNNEDLIQSWHLDGYSFFVVGMDGGQWTSNSRNQYNLRDAIARCTTQVYPK 480
           YRA+VEIVFQNNEDLIQSWHLDGYSFFVVGMDGGQWTSNSRNQYNLRDAIARCTTQVYPK
Sbjct: 421 YRAFVEIVFQNNEDLIQSWHLDGYSFFVVGMDGGQWTSNSRNQYNLRDAIARCTTQVYPK 480

Query: 481 SWTAIYVALDNVGMWNVRSEFWARQYLGQQFYLRVYTPSTSLRDEYPIPKNALLCGRATG 540
           SWTAIYVALDNVGMWN+RSEFWARQYLGQQFYLRVYTPSTSLRDEYPIPKNALLCGRATG
Sbjct: 481 SWTAIYVALDNVGMWNLRSEFWARQYLGQQFYLRVYTPSTSLRDEYPIPKNALLCGRATG 540

Query: 541 RRTRPL 542
           RRTRPL
Sbjct: 541 RRTRPL 546

BLAST of MS016062 vs. NCBI nr
Match: XP_022997333.1 (L-ascorbate oxidase homolog [Cucurbita maxima])

HSP 1 Score: 1092.0 bits (2823), Expect = 0.0e+00
Identity = 521/546 (95.42%), Postives = 534/546 (97.80%), Query Frame = 0

Query: 1   MAIAS-----APLLCLCVSFFAIVAADDPYRFFNWNVTYGTIYPLGLPQQGILINGQFPG 60
           MAI+S      PLLCLC+SFFAIV ADDPYRFFNWNVTYGTIYPLGLPQQGILINGQFPG
Sbjct: 1   MAISSMRSSLPPLLCLCISFFAIVGADDPYRFFNWNVTYGTIYPLGLPQQGILINGQFPG 60

Query: 61  PDIHSVTNDNLIINVFNSLDEPFLLSWNGIQQRRNSYEDGVYGTTCPIPPGKNFTYILQV 120
           PDIHSVTNDN+IINV+NSLDEPFLLSWNGIQQRRNSYEDGVYGTTCPIPPGKNFTYILQ+
Sbjct: 61  PDIHSVTNDNIIINVYNSLDEPFLLSWNGIQQRRNSYEDGVYGTTCPIPPGKNFTYILQM 120

Query: 121 KDQIGSFYYFPSLSFHKAAGGFGGIRILSRPRIPVPFPDPAGDYTVLIGDWYQANHTTLR 180
           KDQIGSFYYFPSL FHKAAGGFGGIRILSRPRIPVPFPDPAGDYTVL+GDWYQANHTTLR
Sbjct: 121 KDQIGSFYYFPSLGFHKAAGGFGGIRILSRPRIPVPFPDPAGDYTVLVGDWYQANHTTLR 180

Query: 181 AQLDNGTMLPLPDGILINGRGPNRTAPFNVEQGKTYRLRISNVGLQNSLNFRIQGHRLKV 240
           AQLDNG+MLPLPDGILINGRGPNRTAPFNVEQGKTYRLRISNVGLQ+SLNFRIQGHRLKV
Sbjct: 181 AQLDNGSMLPLPDGILINGRGPNRTAPFNVEQGKTYRLRISNVGLQSSLNFRIQGHRLKV 240

Query: 241 VEVEGTHTLQTEFSSLDIHVGQSYSVLVTADQPAQDFYVVVSSRFTTPILTTTAILRYAN 300
           VEVEGTH+LQTEFSSLDIHVGQSYSVLVTADQPAQDFY+VVSSRFTTPILTTT ILRYAN
Sbjct: 241 VEVEGTHSLQTEFSSLDIHVGQSYSVLVTADQPAQDFYIVVSSRFTTPILTTTTILRYAN 300

Query: 301 SAGSVQGPPPGGPTIQIDWSLNQARAIRTNLTASGPRPNPQGSYHYGLINTTKTIVLSNS 360
           SAGSVQGPPPGGPTIQIDWSLNQARAIRTNLTASGPRPNPQGSYHYGLINTTKTIVLSNS
Sbjct: 301 SAGSVQGPPPGGPTIQIDWSLNQARAIRTNLTASGPRPNPQGSYHYGLINTTKTIVLSNS 360

Query: 361 AGFVNGKQRYAVNSVSFIPADTPLKVADFFKIGGVFRVGSISDWPTGGGIYQDTSVMGAD 420
           AG VNGKQRYAVNSVSFIPADTPLKVADFFKIGGVFRVGSISDWP G GIY+DTSVMGAD
Sbjct: 361 AGLVNGKQRYAVNSVSFIPADTPLKVADFFKIGGVFRVGSISDWPNGAGIYEDTSVMGAD 420

Query: 421 YRAYVEIVFQNNEDLIQSWHLDGYSFFVVGMDGGQWTSNSRNQYNLRDAIARCTTQVYPK 480
           YRA+VEIVFQNNEDLIQSWHLDGYSFFVVGMDGGQWTSNSRNQYNLRDAIARCTTQVYPK
Sbjct: 421 YRAFVEIVFQNNEDLIQSWHLDGYSFFVVGMDGGQWTSNSRNQYNLRDAIARCTTQVYPK 480

Query: 481 SWTAIYVALDNVGMWNVRSEFWARQYLGQQFYLRVYTPSTSLRDEYPIPKNALLCGRATG 540
           SWTAIYVALDNVGMWN+RSEFWARQYLGQQFYLRVYTPSTSLRDEYPIPKNALLCGRATG
Sbjct: 481 SWTAIYVALDNVGMWNLRSEFWARQYLGQQFYLRVYTPSTSLRDEYPIPKNALLCGRATG 540

Query: 541 RRTRPL 542
           RRTRPL
Sbjct: 541 RRTRPL 546

BLAST of MS016062 vs. NCBI nr
Match: XP_022961945.1 (L-ascorbate oxidase homolog [Cucurbita moschata])

HSP 1 Score: 1091.3 bits (2821), Expect = 0.0e+00
Identity = 522/546 (95.60%), Postives = 533/546 (97.62%), Query Frame = 0

Query: 1   MAIAS-----APLLCLCVSFFAIVAADDPYRFFNWNVTYGTIYPLGLPQQGILINGQFPG 60
           MAI+S      PLLCLC+SFFAIV ADDPYRFFNWNVTYGTIYPLGLPQQGILINGQFPG
Sbjct: 1   MAISSMRSSLPPLLCLCISFFAIVGADDPYRFFNWNVTYGTIYPLGLPQQGILINGQFPG 60

Query: 61  PDIHSVTNDNLIINVFNSLDEPFLLSWNGIQQRRNSYEDGVYGTTCPIPPGKNFTYILQV 120
           PDIHSVTNDN+IINVFNSLDEPFLLSWNGIQQRRNSYEDGVYGTTCPIPPGKNFTYILQ+
Sbjct: 61  PDIHSVTNDNIIINVFNSLDEPFLLSWNGIQQRRNSYEDGVYGTTCPIPPGKNFTYILQM 120

Query: 121 KDQIGSFYYFPSLSFHKAAGGFGGIRILSRPRIPVPFPDPAGDYTVLIGDWYQANHTTLR 180
           KDQIGSFYYFPSL FHKAAGGFGGIRILSRPRIPVPFPDPAGDYTVLIGDWYQANHTTLR
Sbjct: 121 KDQIGSFYYFPSLGFHKAAGGFGGIRILSRPRIPVPFPDPAGDYTVLIGDWYQANHTTLR 180

Query: 181 AQLDNGTMLPLPDGILINGRGPNRTAPFNVEQGKTYRLRISNVGLQNSLNFRIQGHRLKV 240
           AQLDNG+MLPLPDGILINGRGPNRTAPFNVEQGKTYRLRISNVGLQ+SLNFRIQGHRLKV
Sbjct: 181 AQLDNGSMLPLPDGILINGRGPNRTAPFNVEQGKTYRLRISNVGLQSSLNFRIQGHRLKV 240

Query: 241 VEVEGTHTLQTEFSSLDIHVGQSYSVLVTADQPAQDFYVVVSSRFTTPILTTTAILRYAN 300
           VEVEGTH+LQTEFSSLDIHVGQSYSVLVTADQPAQDFY+VVSSRFTTPILTTT ILRYAN
Sbjct: 241 VEVEGTHSLQTEFSSLDIHVGQSYSVLVTADQPAQDFYIVVSSRFTTPILTTTTILRYAN 300

Query: 301 SAGSVQGPPPGGPTIQIDWSLNQARAIRTNLTASGPRPNPQGSYHYGLINTTKTIVLSNS 360
           SAGSVQGPPPGGPTIQIDWSLNQARAIRTNLTASGPRPNPQGSYHYGLI TTKTIVLSNS
Sbjct: 301 SAGSVQGPPPGGPTIQIDWSLNQARAIRTNLTASGPRPNPQGSYHYGLITTTKTIVLSNS 360

Query: 361 AGFVNGKQRYAVNSVSFIPADTPLKVADFFKIGGVFRVGSISDWPTGGGIYQDTSVMGAD 420
           AG VNGKQRYAVNSVSFIPADTPLKVADFFKIGGVFRVGSISDWP G GIY+DTSVMGAD
Sbjct: 361 AGLVNGKQRYAVNSVSFIPADTPLKVADFFKIGGVFRVGSISDWPNGAGIYEDTSVMGAD 420

Query: 421 YRAYVEIVFQNNEDLIQSWHLDGYSFFVVGMDGGQWTSNSRNQYNLRDAIARCTTQVYPK 480
           YRA+VEIVFQNNEDLIQSWHLDGYSFFVVGMDGGQWTSNSRNQYNLRDAIARCTTQVYPK
Sbjct: 421 YRAFVEIVFQNNEDLIQSWHLDGYSFFVVGMDGGQWTSNSRNQYNLRDAIARCTTQVYPK 480

Query: 481 SWTAIYVALDNVGMWNVRSEFWARQYLGQQFYLRVYTPSTSLRDEYPIPKNALLCGRATG 540
           SWTAIYVALDNVGMWN+RSEFWARQYLGQQFYLRVYTPSTSLRDEYPIPKNALLCGRATG
Sbjct: 481 SWTAIYVALDNVGMWNLRSEFWARQYLGQQFYLRVYTPSTSLRDEYPIPKNALLCGRATG 540

Query: 541 RRTRPL 542
           RRTRPL
Sbjct: 541 RRTRPL 546

BLAST of MS016062 vs. ExPASy Swiss-Prot
Match: Q00624 (L-ascorbate oxidase homolog OS=Brassica napus OX=3708 GN=Bp10 PE=2 SV=1)

HSP 1 Score: 608.6 bits (1568), Expect = 6.8e-173
Identity = 293/534 (54.87%), Postives = 378/534 (70.79%), Query Frame = 0

Query: 6   APLLCLCVSFFAIVAADDPYRFFNWNVTYGTIYPLGLPQQGILINGQFPGPDIHSVTNDN 65
           A  L L  +   +V A+DPY    WNVTYGT  PLG+PQQ ILINGQFPGP+I+S +N+N
Sbjct: 8   AACLYLAAAATVVVHAEDPYFHHVWNVTYGTASPLGVPQQVILINGQFPGPNINSTSNNN 67

Query: 66  LIINVFNSLDEPFLLSWNGIQQRRNSYEDGVYGTTCPIPPGKNFTYILQVKDQIGSFYYF 125
           +IINVFN+LDEPFLL+WNGIQ R+N ++DG  GT CPI PG N+TY  Q KDQIGS++Y+
Sbjct: 68  VIINVFNNLDEPFLLTWNGIQHRKNCWQDGTPGTMCPIMPGTNYTYHFQPKDQIGSYFYY 127

Query: 126 PSLSFHKAAGGFGGIRILSRPRIPVPFPDPAGDYTVLIGDWYQANHTTLRAQLDNGTMLP 185
           P+   H+AAGG+GG+R+ SR  IPVP+ DP  DYTVLIGDWY  +HT L+  LD G  + 
Sbjct: 128 PTTGMHRAAGGYGGLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHTQLKKFLDGGRTIG 187

Query: 186 LPDGILIN---GRGPNRTAP-FNVEQGKTYRLRISNVGLQNSLNFRIQGHRLKVVEVEGT 245
            PDGI+IN   G+G    AP F ++ GKTYR+RI NVG++ S+NFRIQ H++K+VE+EG+
Sbjct: 188 RPDGIVINGKSGKGDGSDAPLFTLKPGKTYRVRICNVGVKTSINFRIQNHKMKLVEMEGS 247

Query: 246 HTLQTEFSSLDIHVGQSYSVLVTADQPAQDFYVVVSSRFTTPILTTTAILRYANSAGSVQ 305
           H LQ ++ SLD+HVGQ +  +VTA+Q  +D+Y+V SSRF   ++TTT +LRY    G   
Sbjct: 248 HVLQNDYDSLDVHVGQCFGTIVTANQEPKDYYMVASSRFLKTVITTTGLLRYEGGKGPAS 307

Query: 306 GPPPGGPTIQIDWSLNQARAIRTNLTASGPRPNPQGSYHYGLINTTKTIVLSNSAGFVNG 365
              P GP +   WSLNQ R+ R NLTAS  RPNPQGSYHYG IN T+TI L N+ G V+G
Sbjct: 308 SQLPAGP-VGWAWSLNQFRSFRWNLTASAARPNPQGSYHYGKINITRTIKLVNTQGKVDG 367

Query: 366 KQRYAVNSVSFIPADTPLKVADFFKIGG-VFRVGSISDWPTGGGIYQ---DTSVMGADYR 425
           K R+A+N VS    +TPLK+A++F I   VF+  +I+D PT   I     + +V+   +R
Sbjct: 368 KLRFALNGVSHTEPETPLKLAEYFGISDKVFKYDTITDDPTPEQIKNIKIEPNVLNITHR 427

Query: 426 AYVEIVFQNNEDLIQSWHLDGYSFFVVGMDGGQWTSNSRNQYNLRDAIARCTTQVYPKSW 485
            +VE+VF+N+E  +QSWHLDGYSFF V ++ G WT   R  YNL DA++R T QVYPK W
Sbjct: 428 TFVEVVFENHEKSVQSWHLDGYSFFSVAVEPGTWTPEKRKNYNLLDAVSRHTVQVYPKCW 487

Query: 486 TAIYVALDNVGMWNVRSEFWARQYLGQQFYLRVYTPSTSLRDEYPIPKNALLCG 532
            AI +  DN GMWNVRSE   R+YLGQQ Y  V +P  SLRDEY +P+ +L CG
Sbjct: 488 AAILLTFDNCGMWNVRSENTERRYLGQQLYASVLSPEKSLRDEYNMPETSLQCG 540

BLAST of MS016062 vs. ExPASy Swiss-Prot
Match: P29162 (L-ascorbate oxidase homolog OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 595.9 bits (1535), Expect = 4.6e-169
Identity = 287/540 (53.15%), Postives = 370/540 (68.52%), Query Frame = 0

Query: 6   APLLCLCVSFFAIVAADDPYRFFNWNVTYGTIYPLGLPQQGILINGQFPGPDIHSVTNDN 65
           A LLCL V     V A+DPY +FNWNVTYGTI PLG+PQQGILINGQFPGP I+  +N+N
Sbjct: 10  ALLLCLSVG----VIAEDPYLYFNWNVTYGTIAPLGVPQQGILINGQFPGPRINCTSNNN 69

Query: 66  LIINVFNSLDEPFLLSWNGIQQRRNSYEDGVYGTTCPIPPGKNFTYILQVKDQIGSFYYF 125
           +++NVFN+LDEPFL +WNG+Q R+NS++DG  GT CPI PG+NFTY  QVKDQIGS+ YF
Sbjct: 70  IVVNVFNNLDEPFLFTWNGVQHRKNSWQDGTPGTMCPIMPGQNFTYRFQVKDQIGSYSYF 129

Query: 126 PSLSFHKAAGGFGGIRILSRPRIPVPFPDPAGDYTVLIGDWYQANHTTLRAQLDNGTMLP 185
           P+ + H+AAGG+G + + SR  IPVPF +PA +Y V +GDWY   H TL+  LD G  + 
Sbjct: 130 PTTALHRAAGGYGALNVHSRALIPVPFDNPADEYNVFVGDWYNKGHKTLKKILDGGRTIG 189

Query: 186 LPDGILINGR----GPNRTAPFNVEQGKTYRLRISNVGLQNSLNFRIQGHRLKVVEVEGT 245
            PDGI+ING+    G  +   F +E GKTYR R  N+G+++S+N R QGH +K+VE+EG+
Sbjct: 190 RPDGIIINGKSAKVGEAKEPLFTMEAGKTYRYRFCNLGMRSSVNIRFQGHPMKLVELEGS 249

Query: 246 HTLQTEFSSLDIHVGQSYSVLVTADQPAQDFYVVVSSRFTTPILTTTAILRYANSAGSVQ 305
           HT+Q  + SLD+HVGQ  SVLVTADQ  +D+Y+VVSSRF    L++ AI+RYAN  G   
Sbjct: 250 HTVQNIYDSLDLHVGQCLSVLVTADQEPKDYYLVVSSRFLKQALSSVAIIRYANGKGPAS 309

Query: 306 ---GPPPGGPTIQIDWSLNQARAIRTNLTASGPRPNPQGSYHYGLINTTKTIVLSNSAGF 365
                PP   T  I WS+NQ R+ R NLTAS  RPNPQGSYHYG IN T+TI + NS   
Sbjct: 310 PELPTPPPENTEGIAWSMNQFRSFRWNLTASAARPNPQGSYHYGQINITRTIKIFNSMSQ 369

Query: 366 VNGKQRYAVNSVSFIPADTPLKVADFF-KIGGVFRVGSISDWPTG--GGIYQDTSVMGAD 425
           V GK RY +N +S    +TPLK+ ++F      F+   ++D        +   T+V  A 
Sbjct: 370 VGGKLRYGLNGISHTNGETPLKLVEYFGATNKAFKYDLMADEAPADPSKLTIATNVKNAT 429

Query: 426 YRAYVEIVFQNNEDLIQSWHLDGYSFFVVGMDGGQWTSNSRNQYNLRDAIARCTTQVYPK 485
           YR +VEI+F+N+E  I+++HLDGYSFF V ++ G+W+   R  YNL D ++R   QVYP 
Sbjct: 430 YRNFVEIIFENHEKTIRTYHLDGYSFFAVAVEPGRWSPEKRKNYNLVDGLSRNNIQVYPN 489

Query: 486 SWTAIYVALDNVGMWNVRSEFWARQYLGQQFYLRVYTPSTSLRDEYPIPKNALLCGRATG 536
           SW AI +  DN GMWN+RSE W + YLG+Q Y  V +PS SLRDEY IP N  LCG   G
Sbjct: 490 SWAAIMLTFDNAGMWNLRSEMWEKTYLGEQLYFSVLSPSRSLRDEYNIPDNHPLCGIVKG 545

BLAST of MS016062 vs. ExPASy Swiss-Prot
Match: Q9SU40 (Monocopper oxidase-like protein SKU5 OS=Arabidopsis thaliana OX=3702 GN=SKU5 PE=1 SV=1)

HSP 1 Score: 548.9 bits (1413), Expect = 6.4e-155
Identity = 280/543 (51.57%), Postives = 358/543 (65.93%), Query Frame = 0

Query: 11  LCVSFFAIVA---ADDPYRFFNWNVTYGTIYPLGLPQQGILINGQFPGPDIHSVTNDNLI 70
           L + FF  ++   A DPY F+N+ V+Y T  PLG+PQQ I ING+FPGP I+  TN+NL+
Sbjct: 7   LLLVFFVNISFCFAADPYSFYNFEVSYITASPLGVPQQVIAINGKFPGPTINVTTNENLV 66

Query: 71  INVFNSLDEPFLLSWNGIQQRRNSYEDGVYGTTCPIPPGKNFTYILQVKDQIGSFYYFPS 130
           +NV N LDE  LL WNGIQQRR S++DGV GT CPIPP  N+TY  QVKDQIGSF+YFPS
Sbjct: 67  VNVRNKLDEGLLLHWNGIQQRRVSWQDGVLGTNCPIPPKWNWTYEFQVKDQIGSFFYFPS 126

Query: 131 LSFHKAAGGFGGIRILSRPRIPVPFPDPAGDYTVLIGDWYQANHTTLRAQLDNGTMLPLP 190
           L F +A+GGFG   +  R  IPVPF  P GD TV IGDWY  NHT LR  LD+G  L +P
Sbjct: 127 LHFQRASGGFGSFVVNPRAIIPVPFSTPDGDITVTIGDWYIRNHTALRKALDDGKDLGMP 186

Query: 191 DGILINGRGPNR-----------TAPFNVEQGKTYRLRISNVGLQNSLNFRIQGHRLKVV 250
           DG+LING+GP R                V  GKTYRLR+SNVG+  SLNFRIQGH L + 
Sbjct: 187 DGVLINGKGPYRYNDTLVADGIDFETITVHPGKTYRLRVSNVGISTSLNFRIQGHNLVLA 246

Query: 251 EVEGTHTLQTEFSSLDIHVGQSYSVLVTADQPA-QDFYVVVSSRFTTPIL----TTTAIL 310
           E EG++T+Q  ++SLDIHVGQSYS LVT DQ A  D+Y+V S+R     +    T   IL
Sbjct: 247 ESEGSYTVQQNYTSLDIHVGQSYSFLVTMDQNASSDYYIVASARVVNETIWRRVTGVGIL 306

Query: 311 RYANSAGSVQGPPPGGPTIQID--WSLNQARAIRTNLTASGPRPNPQGSYHYGLINTTKT 370
           +Y NS G  +G  P GP  + D  +S+NQAR+IR N++ASG RPNPQGS+ YG IN T  
Sbjct: 307 KYTNSKGKAKGQLPPGPQDEFDKTFSMNQARSIRWNVSASGARPNPQGSFKYGSINVTDV 366

Query: 371 IVLSNSAGF-VNGKQRYAVNSVSFIPADTPLKVADFFKIGGVFRVGSISDWPTGGGIYQD 430
            VL N     ++GK+R  +N +SF    TP+++AD  K+  V+++      P  G     
Sbjct: 367 YVLRNMPPVTISGKRRTTLNGISFKNPSTPIRLADKLKVKDVYKL-DFPKRPLTGPAKVA 426

Query: 431 TSVMGADYRAYVEIVFQNNEDLIQSWHLDGYSFFVVGMDGGQWTSNSRNQYNLRDAIARC 490
           TS++   YR ++E+V QNN+  +QS+H+ GY+FFVVGMD G+WT NSR  YN  D IAR 
Sbjct: 427 TSIINGTYRGFMEVVLQNNDTKMQSYHMSGYAFFVVGMDYGEWTENSRGTYNKWDGIARS 486

Query: 491 TTQVYPKSWTAIYVALDNVGMWNVRSEFWARQYLGQQFYLRVYTPSTSLRDEYPIPKNAL 532
           T QVYP +W+AI ++LDN G WN+R+E     YLGQ+ Y+RV  P  + + E+  P N L
Sbjct: 487 TIQVYPGAWSAILISLDNPGAWNLRTENLDSWYLGQETYVRVVNPDENNKTEFGHPDNVL 546

BLAST of MS016062 vs. ExPASy Swiss-Prot
Match: Q8VXX5 (Monocopper oxidase-like protein SKS1 OS=Arabidopsis thaliana OX=3702 GN=SKS1 PE=2 SV=1)

HSP 1 Score: 542.3 bits (1396), Expect = 6.0e-153
Identity = 272/542 (50.18%), Postives = 361/542 (66.61%), Query Frame = 0

Query: 8   LLCLCVSFFAIVAADDPYRFFNWNVTYGTIYPLGLPQQGILINGQFPGPDIHSVTNDNLI 67
           LLC  +   A+  A DP+  +++ V+Y T  PLG+PQQ I +NGQFPGP +++ TN N++
Sbjct: 12  LLCFAL-LSAVSFAADPFVSYDFRVSYLTASPLGVPQQVIAVNGQFPGPLLNATTNYNVV 71

Query: 68  INVFNSLDEPFLLSWNGIQQRRNSYEDGVYGTTCPIPPGKNFTYILQVKDQIGSFYYFPS 127
           +NVFN LDEP LL+W GIQ RRNS++DGV GT CPIPP  NFTY  QVKDQIGSF+Y PS
Sbjct: 72  VNVFNHLDEPLLLTWPGIQMRRNSWQDGVLGTNCPIPPRWNFTYQFQVKDQIGSFFYSPS 131

Query: 128 LSFHKAAGGFGGIRILSRPRIPVPFPDPAGDYTVLIGDWYQANHTTLRAQLDNGTMLPLP 187
           L+F +A+GGFG I I +R  IP+PFP P G+   +IGDWY  +H  LR  LD+G  L +P
Sbjct: 132 LNFQRASGGFGPIVINNRDIIPIPFPQPDGELIFIIGDWYTQDHKALRRALDSGKELGMP 191

Query: 188 DGILINGRGP---NRTAP-------FNVEQGKTYRLRISNVGLQNSLNFRIQGHRLKVVE 247
           DG+LING+GP   N + P       F+VE GKTYR+R+ NVG+  SLNFRIQ H L +VE
Sbjct: 192 DGVLINGKGPYKYNSSVPDGIDYLTFHVEPGKTYRIRVHNVGISTSLNFRIQNHSLLLVE 251

Query: 248 VEGTHTLQTEFSSLDIHVGQSYSVLVTADQPA-QDFYVVVSSRFTTPI----LTTTAILR 307
            EG +T Q  F+  D+HVGQSYS LVT DQ A  D+Y+V S+RF        +T  AIL 
Sbjct: 252 TEGHYTSQANFTDFDVHVGQSYSFLVTMDQDATSDYYIVASARFVNETVWQRVTGVAILH 311

Query: 308 YANSAGSVQGPPPGGPT-IQIDWS-LNQARAIRTNLTASGPRPNPQGSYHYGLINTTKTI 367
           Y+NS G V GP P   T +   WS ++Q + IR N +ASG RPNPQGS+HYG IN T T 
Sbjct: 312 YSNSKGPVSGPLPVPKTDVSSPWSAMSQPKTIRQNTSASGARPNPQGSFHYGQINITNTY 371

Query: 368 VL-SNSAGFVNGKQRYAVNSVSFIPADTPLKVADFFKIGGVFRVGSISDWPTGGGIYQDT 427
           +L S     +NG  R  +N +SF+   TP+++AD  K+ G +++    D P    +  D 
Sbjct: 372 ILRSLPPTIINGALRATLNGISFVNPSTPVRLADRNKVKGAYKL-DFPDRPFNRPLRLDR 431

Query: 428 SVMGADYRAYVEIVFQNNEDLIQSWHLDGYSFFVVGMDGGQWTSNSRNQYNLRDAIARCT 487
           S++ A Y+ ++++VFQNN+  IQS+H+DGYSFFVVGMD G W+ + +  YN  DAI+R T
Sbjct: 432 SMINATYKGFIQVVFQNNDTKIQSFHVDGYSFFVVGMDFGIWSEDKKGSYNNWDAISRST 491

Query: 488 TQVYPKSWTAIYVALDNVGMWNVRSEFWARQYLGQQFYLRVYTPSTSLRDEYPIPKNALL 532
            +VYP  WTA+ ++LDNVG+WN+R E   R YLG++ Y+R+  P    + E   P N L 
Sbjct: 492 IEVYPGGWTAVLISLDNVGVWNIRVENLDRWYLGEETYMRITNPEEDGKTEMDPPDNVLY 551

BLAST of MS016062 vs. ExPASy Swiss-Prot
Match: Q9FHN6 (Monocopper oxidase-like protein SKS2 OS=Arabidopsis thaliana OX=3702 GN=SKS2 PE=2 SV=1)

HSP 1 Score: 529.3 bits (1362), Expect = 5.3e-149
Identity = 258/535 (48.22%), Postives = 357/535 (66.73%), Query Frame = 0

Query: 16  FAIVAADDPYRFFNWNVTYGTIYPLGLPQQGILINGQFPGPDIHSVTNDNLIINVFNSLD 75
           F    A DPY  +++ ++Y T  PLG+PQQ I +NG+FPGP I++ TN N+ +NV N LD
Sbjct: 18  FGFSFAGDPYVSYDFTLSYITASPLGVPQQVIAVNGKFPGPVINATTNYNVHVNVLNHLD 77

Query: 76  EPFLLSWNGIQQRRNSYEDGVYGTTCPIPPGKNFTYILQVKDQIGSFYYFPSLSFHKAAG 135
           EP LL+W G+Q RRNS++DGV GT CPIPP  NFTY  Q+KDQIGS++Y PSL+F +A+G
Sbjct: 78  EPLLLTWPGVQMRRNSWQDGVLGTNCPIPPNWNFTYDFQLKDQIGSYFYSPSLNFQRASG 137

Query: 136 GFGGIRILSRPRIPVPFPDPAGDYTVLIGDWYQANHTTLRAQLDNGTMLPLPDGILINGR 195
           GFG + I +R  +P+PF +P G+   +IGDWY  NHT LR  LD+G  L +PDG+LING+
Sbjct: 138 GFGALIINNRDLVPIPFTEPDGEIIFIIGDWYTQNHTALRRILDSGKELGMPDGVLINGK 197

Query: 196 GP---NRTAP-------FNVEQGKTYRLRISNVGLQNSLNFRIQGHRLKVVEVEGTHTLQ 255
           GP   N + P        NV+ GKTYR+R+ NVG+  SLNFRIQ H+L ++E EG +T Q
Sbjct: 198 GPFKYNSSVPDGIEHETVNVDPGKTYRIRVHNVGISTSLNFRIQNHKLLLIETEGRYTSQ 257

Query: 256 TEFSSLDIHVGQSYSVLVTADQPA-QDFYVVVSSRFTTPI----LTTTAILRYANSAGSV 315
             F+  D+HVGQSYS LVT DQ A  D+Y+V S+RF        +T   IL Y+NS G  
Sbjct: 258 MNFTDFDVHVGQSYSFLVTMDQNATSDYYIVASARFVNETVWQRVTGVGILHYSNSKGPA 317

Query: 316 QGPPPGGPT-IQIDWS-LNQARAIRTNLTASGPRPNPQGSYHYGLINTTKTIVL-SNSAG 375
            GP P   T +   WS +NQ RAI+ N +ASG RPNPQGS+HYG IN T+T +L S    
Sbjct: 318 SGPLPVSATDVNHPWSAMNQPRAIKQNTSASGARPNPQGSFHYGQINITRTYILRSLPPT 377

Query: 376 FVNGKQRYAVNSVSFIPADTPLKVADFFKIGGVFRVGSISDWPTGGGIYQ-DTSVMGADY 435
            +NGK R  +N +SF+   TP+++AD  K+ G + +    D P    + +  +S++ A Y
Sbjct: 378 KINGKLRATLNGISFVNPSTPMRLADDHKVKGDYML-DFPDRPLDEKLPRLSSSIINATY 437

Query: 436 RAYVEIVFQNNEDLIQSWHLDGYSFFVVGMDGGQWTSNSRNQYNLRDAIARCTTQVYPKS 495
           + +++++FQNN+  IQS+H+DGY+F+VV MD G W+ +  + YN  DA+AR T +VYP +
Sbjct: 438 KGFIQVIFQNNDTKIQSFHIDGYAFYVVAMDFGIWSEDRNSSYNNWDAVARSTVEVYPGA 497

Query: 496 WTAIYVALDNVGMWNVRSEFWARQYLGQQFYLRVYTPSTSLRDEYPIPKNALLCG 532
           WTA+ ++LDNVG+WN+R E   R YLGQ+ Y+R+  P  +   E   P+N + CG
Sbjct: 498 WTAVLISLDNVGVWNIRVENLDRWYLGQETYMRIINPEENGSTEMDPPENVMYCG 551

BLAST of MS016062 vs. ExPASy TrEMBL
Match: A0A6J1CRH9 (L-ascorbate oxidase homolog OS=Momordica charantia OX=3673 GN=LOC111014083 PE=3 SV=1)

HSP 1 Score: 1121.7 bits (2900), Expect = 0.0e+00
Identity = 539/541 (99.63%), Postives = 539/541 (99.63%), Query Frame = 0

Query: 1   MAIASAPLLCLCVSFFAIVAADDPYRFFNWNVTYGTIYPLGLPQQGILINGQFPGPDIHS 60
           MAIASAPLLCLCVSFFAIVAADDPYRFFNWNVTYGTIYPLGLPQQGILINGQFPGPDIHS
Sbjct: 1   MAIASAPLLCLCVSFFAIVAADDPYRFFNWNVTYGTIYPLGLPQQGILINGQFPGPDIHS 60

Query: 61  VTNDNLIINVFNSLDEPFLLSWNGIQQRRNSYEDGVYGTTCPIPPGKNFTYILQVKDQIG 120
           VTNDNLIINVFNSLDEPFLLSWNGIQQRRNSYEDGVYGTTCPIPPGKNFTYILQVKDQIG
Sbjct: 61  VTNDNLIINVFNSLDEPFLLSWNGIQQRRNSYEDGVYGTTCPIPPGKNFTYILQVKDQIG 120

Query: 121 SFYYFPSLSFHKAAGGFGGIRILSRPRIPVPFPDPAGDYTVLIGDWYQANHTTLRAQLDN 180
           SFYYFPSL FHKAAGGFGGIRILSRPRIPVPFPDPAGDYTVLIGDWYQANHTTLRAQLDN
Sbjct: 121 SFYYFPSLGFHKAAGGFGGIRILSRPRIPVPFPDPAGDYTVLIGDWYQANHTTLRAQLDN 180

Query: 181 GTMLPLPDGILINGRGPNRTAPFNVEQGKTYRLRISNVGLQNSLNFRIQGHRLKVVEVEG 240
           GTMLPLPDGILINGRGPNRTAPFNVEQGKTYRLRISNVGLQNSLNFRIQGHRLKVVEVEG
Sbjct: 181 GTMLPLPDGILINGRGPNRTAPFNVEQGKTYRLRISNVGLQNSLNFRIQGHRLKVVEVEG 240

Query: 241 THTLQTEFSSLDIHVGQSYSVLVTADQPAQDFYVVVSSRFTTPILTTTAILRYANSAGSV 300
           THTLQTEFSSLDIHVGQ YSVLVTADQPAQDFYVVVSSRFTTPILTTTAILRYANSAGSV
Sbjct: 241 THTLQTEFSSLDIHVGQFYSVLVTADQPAQDFYVVVSSRFTTPILTTTAILRYANSAGSV 300

Query: 301 QGPPPGGPTIQIDWSLNQARAIRTNLTASGPRPNPQGSYHYGLINTTKTIVLSNSAGFVN 360
           QGPPPGGPTIQIDWSLNQARAIRTNLTASGPRPNPQGSYHYGLINTTKTIVLSNSAGFVN
Sbjct: 301 QGPPPGGPTIQIDWSLNQARAIRTNLTASGPRPNPQGSYHYGLINTTKTIVLSNSAGFVN 360

Query: 361 GKQRYAVNSVSFIPADTPLKVADFFKIGGVFRVGSISDWPTGGGIYQDTSVMGADYRAYV 420
           GKQRYAVNSVSFIPADTPLKVADFFKIGGVFRVGSISDWPTGGGIYQDTSVMGADYRAYV
Sbjct: 361 GKQRYAVNSVSFIPADTPLKVADFFKIGGVFRVGSISDWPTGGGIYQDTSVMGADYRAYV 420

Query: 421 EIVFQNNEDLIQSWHLDGYSFFVVGMDGGQWTSNSRNQYNLRDAIARCTTQVYPKSWTAI 480
           EIVFQNNEDLIQSWHLDGYSFFVVGMDGGQWTSNSRNQYNLRDAIARCTTQVYPKSWTAI
Sbjct: 421 EIVFQNNEDLIQSWHLDGYSFFVVGMDGGQWTSNSRNQYNLRDAIARCTTQVYPKSWTAI 480

Query: 481 YVALDNVGMWNVRSEFWARQYLGQQFYLRVYTPSTSLRDEYPIPKNALLCGRATGRRTRP 540
           YVALDNVGMWNVRSEFWARQYLGQQFYLRVYTPSTSLRDEYPIPKNALLCGRATGRRTRP
Sbjct: 481 YVALDNVGMWNVRSEFWARQYLGQQFYLRVYTPSTSLRDEYPIPKNALLCGRATGRRTRP 540

Query: 541 L 542
           L
Sbjct: 541 L 541

BLAST of MS016062 vs. ExPASy TrEMBL
Match: A0A6J1KDJ5 (L-ascorbate oxidase homolog OS=Cucurbita maxima OX=3661 GN=LOC111492278 PE=3 SV=1)

HSP 1 Score: 1092.0 bits (2823), Expect = 0.0e+00
Identity = 521/546 (95.42%), Postives = 534/546 (97.80%), Query Frame = 0

Query: 1   MAIAS-----APLLCLCVSFFAIVAADDPYRFFNWNVTYGTIYPLGLPQQGILINGQFPG 60
           MAI+S      PLLCLC+SFFAIV ADDPYRFFNWNVTYGTIYPLGLPQQGILINGQFPG
Sbjct: 1   MAISSMRSSLPPLLCLCISFFAIVGADDPYRFFNWNVTYGTIYPLGLPQQGILINGQFPG 60

Query: 61  PDIHSVTNDNLIINVFNSLDEPFLLSWNGIQQRRNSYEDGVYGTTCPIPPGKNFTYILQV 120
           PDIHSVTNDN+IINV+NSLDEPFLLSWNGIQQRRNSYEDGVYGTTCPIPPGKNFTYILQ+
Sbjct: 61  PDIHSVTNDNIIINVYNSLDEPFLLSWNGIQQRRNSYEDGVYGTTCPIPPGKNFTYILQM 120

Query: 121 KDQIGSFYYFPSLSFHKAAGGFGGIRILSRPRIPVPFPDPAGDYTVLIGDWYQANHTTLR 180
           KDQIGSFYYFPSL FHKAAGGFGGIRILSRPRIPVPFPDPAGDYTVL+GDWYQANHTTLR
Sbjct: 121 KDQIGSFYYFPSLGFHKAAGGFGGIRILSRPRIPVPFPDPAGDYTVLVGDWYQANHTTLR 180

Query: 181 AQLDNGTMLPLPDGILINGRGPNRTAPFNVEQGKTYRLRISNVGLQNSLNFRIQGHRLKV 240
           AQLDNG+MLPLPDGILINGRGPNRTAPFNVEQGKTYRLRISNVGLQ+SLNFRIQGHRLKV
Sbjct: 181 AQLDNGSMLPLPDGILINGRGPNRTAPFNVEQGKTYRLRISNVGLQSSLNFRIQGHRLKV 240

Query: 241 VEVEGTHTLQTEFSSLDIHVGQSYSVLVTADQPAQDFYVVVSSRFTTPILTTTAILRYAN 300
           VEVEGTH+LQTEFSSLDIHVGQSYSVLVTADQPAQDFY+VVSSRFTTPILTTT ILRYAN
Sbjct: 241 VEVEGTHSLQTEFSSLDIHVGQSYSVLVTADQPAQDFYIVVSSRFTTPILTTTTILRYAN 300

Query: 301 SAGSVQGPPPGGPTIQIDWSLNQARAIRTNLTASGPRPNPQGSYHYGLINTTKTIVLSNS 360
           SAGSVQGPPPGGPTIQIDWSLNQARAIRTNLTASGPRPNPQGSYHYGLINTTKTIVLSNS
Sbjct: 301 SAGSVQGPPPGGPTIQIDWSLNQARAIRTNLTASGPRPNPQGSYHYGLINTTKTIVLSNS 360

Query: 361 AGFVNGKQRYAVNSVSFIPADTPLKVADFFKIGGVFRVGSISDWPTGGGIYQDTSVMGAD 420
           AG VNGKQRYAVNSVSFIPADTPLKVADFFKIGGVFRVGSISDWP G GIY+DTSVMGAD
Sbjct: 361 AGLVNGKQRYAVNSVSFIPADTPLKVADFFKIGGVFRVGSISDWPNGAGIYEDTSVMGAD 420

Query: 421 YRAYVEIVFQNNEDLIQSWHLDGYSFFVVGMDGGQWTSNSRNQYNLRDAIARCTTQVYPK 480
           YRA+VEIVFQNNEDLIQSWHLDGYSFFVVGMDGGQWTSNSRNQYNLRDAIARCTTQVYPK
Sbjct: 421 YRAFVEIVFQNNEDLIQSWHLDGYSFFVVGMDGGQWTSNSRNQYNLRDAIARCTTQVYPK 480

Query: 481 SWTAIYVALDNVGMWNVRSEFWARQYLGQQFYLRVYTPSTSLRDEYPIPKNALLCGRATG 540
           SWTAIYVALDNVGMWN+RSEFWARQYLGQQFYLRVYTPSTSLRDEYPIPKNALLCGRATG
Sbjct: 481 SWTAIYVALDNVGMWNLRSEFWARQYLGQQFYLRVYTPSTSLRDEYPIPKNALLCGRATG 540

Query: 541 RRTRPL 542
           RRTRPL
Sbjct: 541 RRTRPL 546

BLAST of MS016062 vs. ExPASy TrEMBL
Match: A0A6J1HDN3 (L-ascorbate oxidase homolog OS=Cucurbita moschata OX=3662 GN=LOC111462565 PE=3 SV=1)

HSP 1 Score: 1091.3 bits (2821), Expect = 0.0e+00
Identity = 522/546 (95.60%), Postives = 533/546 (97.62%), Query Frame = 0

Query: 1   MAIAS-----APLLCLCVSFFAIVAADDPYRFFNWNVTYGTIYPLGLPQQGILINGQFPG 60
           MAI+S      PLLCLC+SFFAIV ADDPYRFFNWNVTYGTIYPLGLPQQGILINGQFPG
Sbjct: 1   MAISSMRSSLPPLLCLCISFFAIVGADDPYRFFNWNVTYGTIYPLGLPQQGILINGQFPG 60

Query: 61  PDIHSVTNDNLIINVFNSLDEPFLLSWNGIQQRRNSYEDGVYGTTCPIPPGKNFTYILQV 120
           PDIHSVTNDN+IINVFNSLDEPFLLSWNGIQQRRNSYEDGVYGTTCPIPPGKNFTYILQ+
Sbjct: 61  PDIHSVTNDNIIINVFNSLDEPFLLSWNGIQQRRNSYEDGVYGTTCPIPPGKNFTYILQM 120

Query: 121 KDQIGSFYYFPSLSFHKAAGGFGGIRILSRPRIPVPFPDPAGDYTVLIGDWYQANHTTLR 180
           KDQIGSFYYFPSL FHKAAGGFGGIRILSRPRIPVPFPDPAGDYTVLIGDWYQANHTTLR
Sbjct: 121 KDQIGSFYYFPSLGFHKAAGGFGGIRILSRPRIPVPFPDPAGDYTVLIGDWYQANHTTLR 180

Query: 181 AQLDNGTMLPLPDGILINGRGPNRTAPFNVEQGKTYRLRISNVGLQNSLNFRIQGHRLKV 240
           AQLDNG+MLPLPDGILINGRGPNRTAPFNVEQGKTYRLRISNVGLQ+SLNFRIQGHRLKV
Sbjct: 181 AQLDNGSMLPLPDGILINGRGPNRTAPFNVEQGKTYRLRISNVGLQSSLNFRIQGHRLKV 240

Query: 241 VEVEGTHTLQTEFSSLDIHVGQSYSVLVTADQPAQDFYVVVSSRFTTPILTTTAILRYAN 300
           VEVEGTH+LQTEFSSLDIHVGQSYSVLVTADQPAQDFY+VVSSRFTTPILTTT ILRYAN
Sbjct: 241 VEVEGTHSLQTEFSSLDIHVGQSYSVLVTADQPAQDFYIVVSSRFTTPILTTTTILRYAN 300

Query: 301 SAGSVQGPPPGGPTIQIDWSLNQARAIRTNLTASGPRPNPQGSYHYGLINTTKTIVLSNS 360
           SAGSVQGPPPGGPTIQIDWSLNQARAIRTNLTASGPRPNPQGSYHYGLI TTKTIVLSNS
Sbjct: 301 SAGSVQGPPPGGPTIQIDWSLNQARAIRTNLTASGPRPNPQGSYHYGLITTTKTIVLSNS 360

Query: 361 AGFVNGKQRYAVNSVSFIPADTPLKVADFFKIGGVFRVGSISDWPTGGGIYQDTSVMGAD 420
           AG VNGKQRYAVNSVSFIPADTPLKVADFFKIGGVFRVGSISDWP G GIY+DTSVMGAD
Sbjct: 361 AGLVNGKQRYAVNSVSFIPADTPLKVADFFKIGGVFRVGSISDWPNGAGIYEDTSVMGAD 420

Query: 421 YRAYVEIVFQNNEDLIQSWHLDGYSFFVVGMDGGQWTSNSRNQYNLRDAIARCTTQVYPK 480
           YRA+VEIVFQNNEDLIQSWHLDGYSFFVVGMDGGQWTSNSRNQYNLRDAIARCTTQVYPK
Sbjct: 421 YRAFVEIVFQNNEDLIQSWHLDGYSFFVVGMDGGQWTSNSRNQYNLRDAIARCTTQVYPK 480

Query: 481 SWTAIYVALDNVGMWNVRSEFWARQYLGQQFYLRVYTPSTSLRDEYPIPKNALLCGRATG 540
           SWTAIYVALDNVGMWN+RSEFWARQYLGQQFYLRVYTPSTSLRDEYPIPKNALLCGRATG
Sbjct: 481 SWTAIYVALDNVGMWNLRSEFWARQYLGQQFYLRVYTPSTSLRDEYPIPKNALLCGRATG 540

Query: 541 RRTRPL 542
           RRTRPL
Sbjct: 541 RRTRPL 546

BLAST of MS016062 vs. ExPASy TrEMBL
Match: A0A1S3BCD0 (L-ascorbate oxidase homolog OS=Cucumis melo OX=3656 GN=LOC103488372 PE=3 SV=1)

HSP 1 Score: 1073.5 bits (2775), Expect = 2.0e-310
Identity = 509/534 (95.32%), Postives = 524/534 (98.13%), Query Frame = 0

Query: 8   LLCLCVSFFAIVAADDPYRFFNWNVTYGTIYPLGLPQQGILINGQFPGPDIHSVTNDNLI 67
           LL L +SFFAIV A+DPYRFF+WNVTYGTIYPLGLPQQGILINGQFPGPDIHSVTNDN+I
Sbjct: 5   LLFLFISFFAIVGAEDPYRFFDWNVTYGTIYPLGLPQQGILINGQFPGPDIHSVTNDNII 64

Query: 68  INVFNSLDEPFLLSWNGIQQRRNSYEDGVYGTTCPIPPGKNFTYILQVKDQIGSFYYFPS 127
           INVFNSLDEPFLLSWNGIQQRRNSYEDGVYGTTCPIPPGKNFTYILQ+KDQIGSFYYFPS
Sbjct: 65  INVFNSLDEPFLLSWNGIQQRRNSYEDGVYGTTCPIPPGKNFTYILQMKDQIGSFYYFPS 124

Query: 128 LSFHKAAGGFGGIRILSRPRIPVPFPDPAGDYTVLIGDWYQANHTTLRAQLDNGTMLPLP 187
           L FHKAAGGFGGIRILSRPRIPVPFPDPAGDYTVLIGDWYQANHTTLRAQLDNG+MLPLP
Sbjct: 125 LGFHKAAGGFGGIRILSRPRIPVPFPDPAGDYTVLIGDWYQANHTTLRAQLDNGSMLPLP 184

Query: 188 DGILINGRGPNRTAPFNVEQGKTYRLRISNVGLQNSLNFRIQGHRLKVVEVEGTHTLQTE 247
           DGILINGRGPNRTA  NVEQGKTYRLRISNVGLQ+SLNFRIQGHR+KVVEVEGTHTLQTE
Sbjct: 185 DGILINGRGPNRTASINVEQGKTYRLRISNVGLQSSLNFRIQGHRMKVVEVEGTHTLQTE 244

Query: 248 FSSLDIHVGQSYSVLVTADQPAQDFYVVVSSRFTTPILTTTAILRYANSAGSVQGPPPGG 307
           FSSLD+HVGQSYSVLVTADQPAQDFY+VVSSRFTTPILTTTAILRYANSAGSVQGPPPGG
Sbjct: 245 FSSLDVHVGQSYSVLVTADQPAQDFYIVVSSRFTTPILTTTAILRYANSAGSVQGPPPGG 304

Query: 308 PTIQIDWSLNQARAIRTNLTASGPRPNPQGSYHYGLINTTKTIVLSNSAGFVNGKQRYAV 367
           PTIQIDWSLNQARAIRTNLTASGPRPNPQGSYHYG+INTT+TIVLSNSAG VNGKQRYAV
Sbjct: 305 PTIQIDWSLNQARAIRTNLTASGPRPNPQGSYHYGMINTTRTIVLSNSAGIVNGKQRYAV 364

Query: 368 NSVSFIPADTPLKVADFFKIGGVFRVGSISDWPTGGGIYQDTSVMGADYRAYVEIVFQNN 427
           NSVSFIPADTPLKVADFFKIGGVFRVGSISDWP GGGIYQDTSVMGADYRA+VEIVFQNN
Sbjct: 365 NSVSFIPADTPLKVADFFKIGGVFRVGSISDWPNGGGIYQDTSVMGADYRAFVEIVFQNN 424

Query: 428 EDLIQSWHLDGYSFFVVGMDGGQWTSNSRNQYNLRDAIARCTTQVYPKSWTAIYVALDNV 487
           EDLIQSWH DGYSFFVVGMDGGQWTSNSRNQYNLRDAIARCT QVYPKSW+AIYVALDNV
Sbjct: 425 EDLIQSWHFDGYSFFVVGMDGGQWTSNSRNQYNLRDAIARCTVQVYPKSWSAIYVALDNV 484

Query: 488 GMWNVRSEFWARQYLGQQFYLRVYTPSTSLRDEYPIPKNALLCGRATGRRTRPL 542
           GMWN+RSEFWARQYLGQQFYLRVYTPSTSLRDEYPIPKNALLCGRATGRRTRPL
Sbjct: 485 GMWNIRSEFWARQYLGQQFYLRVYTPSTSLRDEYPIPKNALLCGRATGRRTRPL 538

BLAST of MS016062 vs. ExPASy TrEMBL
Match: A0A0A0LN97 (Multicopper oxidase OS=Cucumis sativus OX=3659 GN=Csa_2G348200 PE=3 SV=1)

HSP 1 Score: 1061.2 bits (2743), Expect = 1.4e-306
Identity = 505/535 (94.39%), Postives = 524/535 (97.94%), Query Frame = 0

Query: 8   LLCLCVS-FFAIVAADDPYRFFNWNVTYGTIYPLGLPQQGILINGQFPGPDIHSVTNDNL 67
           LL L +S FFAIV A+DPYRFF+WNVTYGTIYPLGLPQQGILINGQFPGPDIHSVTNDN+
Sbjct: 5   LLFLFISFFFAIVRAEDPYRFFDWNVTYGTIYPLGLPQQGILINGQFPGPDIHSVTNDNI 64

Query: 68  IINVFNSLDEPFLLSWNGIQQRRNSYEDGVYGTTCPIPPGKNFTYILQVKDQIGSFYYFP 127
           IINVFNSLDEPFLLSWNGIQQRRNSYEDGVYGTTCPIPPGKNFTYILQ+KDQIGSFYYFP
Sbjct: 65  IINVFNSLDEPFLLSWNGIQQRRNSYEDGVYGTTCPIPPGKNFTYILQMKDQIGSFYYFP 124

Query: 128 SLSFHKAAGGFGGIRILSRPRIPVPFPDPAGDYTVLIGDWYQANHTTLRAQLDNGTMLPL 187
           SL FHKAAGGFGGIRILSRPRIPVPFPDPAGDYTVLIGDWYQANHTTLRAQLDNG+MLPL
Sbjct: 125 SLGFHKAAGGFGGIRILSRPRIPVPFPDPAGDYTVLIGDWYQANHTTLRAQLDNGSMLPL 184

Query: 188 PDGILINGRGPNRTAPFNVEQGKTYRLRISNVGLQNSLNFRIQGHRLKVVEVEGTHTLQT 247
           PDGILINGRGPNRTA  NVEQGKTYRLRISNVGLQ+SLNFRIQGHR+KVVEVEGTHTLQT
Sbjct: 185 PDGILINGRGPNRTASINVEQGKTYRLRISNVGLQSSLNFRIQGHRMKVVEVEGTHTLQT 244

Query: 248 EFSSLDIHVGQSYSVLVTADQPAQDFYVVVSSRFTTPILTTTAILRYANSAGSVQGPPPG 307
           EFSSLDIHVGQSYSVLVTADQPAQDFY+VVSSRFTTPIL+TTAILRYANSAGSVQGPPPG
Sbjct: 245 EFSSLDIHVGQSYSVLVTADQPAQDFYIVVSSRFTTPILSTTAILRYANSAGSVQGPPPG 304

Query: 308 GPTIQIDWSLNQARAIRTNLTASGPRPNPQGSYHYGLINTTKTIVLSNSAGFVNGKQRYA 367
           GPTIQIDWSLNQARAIRTNLTASGPRPNPQGSYHYG+I TT+TIV++NSAG V+GKQRYA
Sbjct: 305 GPTIQIDWSLNQARAIRTNLTASGPRPNPQGSYHYGMITTTRTIVVANSAGIVDGKQRYA 364

Query: 368 VNSVSFIPADTPLKVADFFKIGGVFRVGSISDWPTGGGIYQDTSVMGADYRAYVEIVFQN 427
           VNSVSFIPADTPLKVADFFKIGGVFRVGSISDWP GGGIYQDTSVMGADYRA+VEIVFQN
Sbjct: 365 VNSVSFIPADTPLKVADFFKIGGVFRVGSISDWPNGGGIYQDTSVMGADYRAFVEIVFQN 424

Query: 428 NEDLIQSWHLDGYSFFVVGMDGGQWTSNSRNQYNLRDAIARCTTQVYPKSWTAIYVALDN 487
           NEDLIQSWHLDGYSFFVVGMDGGQWTSNSRNQYNLRDAIARCT QVYPKSW+AIYVALDN
Sbjct: 425 NEDLIQSWHLDGYSFFVVGMDGGQWTSNSRNQYNLRDAIARCTVQVYPKSWSAIYVALDN 484

Query: 488 VGMWNVRSEFWARQYLGQQFYLRVYTPSTSLRDEYPIPKNALLCGRATGRRTRPL 542
           VGMWN+R+EFWARQYLGQQFYLRVYTPSTSLRDEYPIPKNALLCGRATGRRTRPL
Sbjct: 485 VGMWNIRTEFWARQYLGQQFYLRVYTPSTSLRDEYPIPKNALLCGRATGRRTRPL 539

BLAST of MS016062 vs. TAIR 10
Match: AT1G76160.1 (SKU5 similar 5 )

HSP 1 Score: 926.8 bits (2394), Expect = 8.1e-270
Identity = 439/536 (81.90%), Postives = 481/536 (89.74%), Query Frame = 0

Query: 6   APLLCLCVSFFAIVAADDPYRFFNWNVTYGTIYPLGLPQQGILINGQFPGPDIHSVTNDN 65
           A  L + +S    V A+DPYRFF WN+TYG IYPLG+ QQGILING FPGPDIHSVTNDN
Sbjct: 8   AAALFIGLSLLFAVTAEDPYRFFEWNITYGDIYPLGVRQQGILINGAFPGPDIHSVTNDN 67

Query: 66  LIINVFNSLDEPFLLSWNGIQQRRNSYEDGVYGTTCPIPPGKNFTYILQVKDQIGSFYYF 125
           LIINV+NSLDEPFLLSWNGIQQRRNS+ DGVYGTTCPIPPGKN+TYILQ+KDQIGSFYYF
Sbjct: 68  LIINVYNSLDEPFLLSWNGIQQRRNSFVDGVYGTTCPIPPGKNYTYILQMKDQIGSFYYF 127

Query: 126 PSLSFHKAAGGFGGIRILSRPRIPVPFPDPAGDYTVLIGDWYQANHTTLRAQLDNGTMLP 185
           PSL FHKAAGGFGGIRILSRPRIPVPFPDPAGD TVLIGDWY+ANHT LRAQLDNG  LP
Sbjct: 128 PSLGFHKAAGGFGGIRILSRPRIPVPFPDPAGDTTVLIGDWYKANHTDLRAQLDNGKKLP 187

Query: 186 LPDGILINGRGPNRTAPFNVEQGKTYRLRISNVGLQNSLNFRIQGHRLKVVEVEGTHTLQ 245
           LPDGILINGR    T   NVEQGKTYR RISNVGLQ+SLNFRIQ H++KVVEVEGTHTLQ
Sbjct: 188 LPDGILINGRSSGAT--LNVEQGKTYRFRISNVGLQDSLNFRIQDHKMKVVEVEGTHTLQ 247

Query: 246 TEFSSLDIHVGQSYSVLVTADQPAQDFYVVVSSRFTTPILTTTAILRYANSAGSVQGPPP 305
           T FSSLD+HVGQSYSVLVTADQ  +D+YVVVSSRFT+ +LTTT I RY+NSAG V GP P
Sbjct: 248 TTFSSLDVHVGQSYSVLVTADQTPRDYYVVVSSRFTSNVLTTTGIFRYSNSAGGVSGPIP 307

Query: 306 GGPTIQIDWSLNQARAIRTNLTASGPRPNPQGSYHYGLINTTKTIVLSNSAGFVNGKQRY 365
           GGPTIQIDWSLNQARAIRTNL+ASGPRPNPQGSYHYG+INTT+TI L++SAG V+GKQRY
Sbjct: 308 GGPTIQIDWSLNQARAIRTNLSASGPRPNPQGSYHYGMINTTRTIRLASSAGQVDGKQRY 367

Query: 366 AVNSVSFIPADTPLKVADFFKIGGVFRVGSISDWPTGGGIYQDTSVMGADYRAYVEIVFQ 425
           AVNSVSF PADTPLK+AD+FKI GV+R GSI   PTGGGIY DTSVM  DYR +VEI+F+
Sbjct: 368 AVNSVSFKPADTPLKIADYFKIDGVYRSGSIQYQPTGGGIYLDTSVMQVDYRTFVEIIFE 427

Query: 426 NNEDLIQSWHLDGYSFFVVGMDGGQWTSNSRNQYNLRDAIARCTTQVYPKSWTAIYVALD 485
           N+ED++QSWHLDGYSF+VVGMDGGQW+ +SRN+YNLRDA+ARCT QVYP SWTAI +ALD
Sbjct: 428 NSEDIVQSWHLDGYSFWVVGMDGGQWSPDSRNEYNLRDAVARCTVQVYPSSWTAILIALD 487

Query: 486 NVGMWNVRSEFWARQYLGQQFYLRVYTPSTSLRDEYPIPKNALLCGRATGRRTRPL 542
           NVGMWN+RSEFWARQYLGQQ YLRVYTPSTSLRDEYPIPKNALLCGRA+GR TRPL
Sbjct: 488 NVGMWNLRSEFWARQYLGQQLYLRVYTPSTSLRDEYPIPKNALLCGRASGRSTRPL 541

BLAST of MS016062 vs. TAIR 10
Match: AT1G41830.1 (SKU5-similar 6 )

HSP 1 Score: 890.6 bits (2300), Expect = 6.4e-259
Identity = 417/534 (78.09%), Postives = 469/534 (87.83%), Query Frame = 0

Query: 8   LLCLCVSFFAIVAADDPYRFFNWNVTYGTIYPLGLPQQGILINGQFPGPDIHSVTNDNLI 67
           +L  C+SFFA V A+ PYRFF+WNVTYG IYPLG+ QQGILINGQFPGPDIHSVTNDNLI
Sbjct: 11  ILLFCLSFFAAVTAESPYRFFDWNVTYGDIYPLGVRQQGILINGQFPGPDIHSVTNDNLI 70

Query: 68  INVFNSLDEPFLLSWNGIQQRRNSYEDGVYGTTCPIPPGKNFTYILQVKDQIGSFYYFPS 127
           INV NSLDEPFL+SWNG+Q RRNSY DG+YGTTCPIPP  N+TYILQVKDQIGSFYYFPS
Sbjct: 71  INVHNSLDEPFLISWNGVQNRRNSYVDGMYGTTCPIPPRSNYTYILQVKDQIGSFYYFPS 130

Query: 128 LSFHKAAGGFGGIRILSRPRIPVPFPDPAGDYTVLIGDWYQANHTTLRAQLDNGTMLPLP 187
           L+FHKAAGGFGGIRILSRP IPVPF DPAGDYTVLIGDWY+ NHT L+++LD G  LP P
Sbjct: 131 LAFHKAAGGFGGIRILSRPGIPVPFADPAGDYTVLIGDWYKFNHTDLKSRLDRGRKLPSP 190

Query: 188 DGILINGRGPNRTAPFNVEQGKTYRLRISNVGLQNSLNFRIQGHRLKVVEVEGTHTLQTE 247
           DGILINGR  +  A  NVEQGKTYRLRISNVGLQ+SLNFRIQ HR+K+VEVEGTHTLQT 
Sbjct: 191 DGILINGR--SNGATLNVEQGKTYRLRISNVGLQDSLNFRIQNHRMKLVEVEGTHTLQTM 250

Query: 248 FSSLDIHVGQSYSVLVTADQPAQDFYVVVSSRFTTPILTTTAILRYANSAGSVQGPPPGG 307
           FSSLD+HVGQSYSVL+TADQ  +D+YVVVSSRFT  I+TTT +LRY+ S+    GP PGG
Sbjct: 251 FSSLDVHVGQSYSVLITADQSPRDYYVVVSSRFTDKIITTTGVLRYSGSSTPASGPIPGG 310

Query: 308 PTIQIDWSLNQARAIRTNLTASGPRPNPQGSYHYGLINTTKTIVLSNSAGFVNGKQRYAV 367
           PTIQ+DWSLNQARAIRTNLTASGPRPNPQGSYHYGLI   +TIV  +SAG +NGKQRY V
Sbjct: 311 PTIQVDWSLNQARAIRTNLTASGPRPNPQGSYHYGLIPLIRTIVFGSSAGQINGKQRYGV 370

Query: 368 NSVSFIPADTPLKVADFFKIGGVFRVGSISDWPTGGGIYQDTSVMGADYRAYVEIVFQNN 427
           NSVSF+PADTPLK+ADFFKI GV+++ SISD PT GG+Y DTSV+  DYR ++EIVF+N 
Sbjct: 371 NSVSFVPADTPLKLADFFKISGVYKINSISDKPTYGGLYLDTSVLQVDYRTFIEIVFENQ 430

Query: 428 EDLIQSWHLDGYSFFVVGMDGGQWTSNSRNQYNLRDAIARCTTQVYPKSWTAIYVALDNV 487
           ED++QS+HL+GYSF+VVGMDGGQW + SRN YNLRDA++R T QVYPKSWTAIY+ALDNV
Sbjct: 431 EDIVQSYHLNGYSFWVVGMDGGQWKTGSRNGYNLRDAVSRSTVQVYPKSWTAIYIALDNV 490

Query: 488 GMWNVRSEFWARQYLGQQFYLRVYTPSTSLRDEYPIPKNALLCGRATGRRTRPL 542
           GMWN+RSEFWARQYLGQQ YLRV+T STSLRDEYPIPKN+ LCGRA GR TRPL
Sbjct: 491 GMWNLRSEFWARQYLGQQLYLRVFTSSTSLRDEYPIPKNSRLCGRARGRHTRPL 542

BLAST of MS016062 vs. TAIR 10
Match: AT1G21850.1 (SKU5 similar 8 )

HSP 1 Score: 839.3 bits (2167), Expect = 1.7e-243
Identity = 397/526 (75.48%), Postives = 449/526 (85.36%), Query Frame = 0

Query: 15  FFAI--VAADDPYRFFNWNVTYGTIYPLGLPQQGILINGQFPGPDIHSVTNDNLIINVFN 74
           FF I  VAA+DPY+FF W+VTYG I PL + QQGILING+FPGPDI +VTNDNLIINVFN
Sbjct: 16  FFLISFVAAEDPYKFFEWHVTYGNISPLKVAQQGILINGKFPGPDIAAVTNDNLIINVFN 75

Query: 75  SLDEPFLLSWNGIQQRRNSYEDGVYGTTCPIPPGKNFTYILQVKDQIGSFYYFPSLSFHK 134
            LDEPFL+SW+GI+  RNSY+DGVYGTTCPIPPGKN+TY LQVKDQIGSFYYFPSL FHK
Sbjct: 76  HLDEPFLISWSGIRNWRNSYQDGVYGTTCPIPPGKNYTYALQVKDQIGSFYYFPSLGFHK 135

Query: 135 AAGGFGGIRILSRPRIPVPFPDPAGDYTVLIGDWYQANHTTLRAQLDNGTMLPLPDGILI 194
           AAGGFG IRI SRPRIPVPFP PAGDYTVLIGDWY+ NH  LRAQLDNG  LP PDGILI
Sbjct: 136 AAGGFGAIRISSRPRIPVPFPAPAGDYTVLIGDWYKTNHKDLRAQLDNGGKLPFPDGILI 195

Query: 195 NGRGPNRTAPFNVEQGKTYRLRISNVGLQNSLNFRIQGHRLKVVEVEGTHTLQTEFSSLD 254
           NGRG   T   N+E GKTYRLRISNVGLQNSLNFRIQ H++K+VEVEGTHT+QT FSSLD
Sbjct: 196 NGRGSGAT--LNIEPGKTYRLRISNVGLQNSLNFRIQNHKMKLVEVEGTHTIQTPFSSLD 255

Query: 255 IHVGQSYSVLVTADQPAQDFYVVVSSRFTTPILTTTAILRYANSAGSVQGPPPGGPTIQI 314
           +HVGQSYSVL+TADQPA+D+Y+VVSSRFT+ IL T  +L Y+NSAG V GP P  P IQ+
Sbjct: 256 VHVGQSYSVLITADQPAKDYYIVVSSRFTSKILITAGVLHYSNSAGPVSGPIPEAP-IQL 315

Query: 315 DWSLNQARAIRTNLTASGPRPNPQGSYHYGLINTTKTIVLSNSAGFVNGKQRYAVNSVSF 374
            WS +QARAI+TNL ASGPRPNPQG+YHYG I  T+TI L++SAG +NGKQRYAVNS SF
Sbjct: 316 RWSFDQARAIKTNLAASGPRPNPQGTYHYGKIKVTRTIKLASSAGNINGKQRYAVNSASF 375

Query: 375 IPADTPLKVADFFKIGGVFRVGSISDWPTGGGIYQDTSVMGADYRAYVEIVFQNNEDLIQ 434
            P DTPLK+AD+FKI GV+  GSI D PT G IY  TSVM  DY+A+VEIVF+N ED++Q
Sbjct: 376 YPTDTPLKLADYFKIAGVYNPGSIPDQPTHGAIYPVTSVMQTDYKAFVEIVFENWEDIVQ 435

Query: 435 SWHLDGYSFFVVGMDGGQWTSNSRNQYNLRDAIARCTTQVYPKSWTAIYVALDNVGMWNV 494
           +WHLDGYSFFVVGM+ G+W++ SR  YNL DA++RCT QVYP+SWTAIYV+LDNVGMWN+
Sbjct: 436 TWHLDGYSFFVVGMELGKWSAASRKVYNLNDAVSRCTVQVYPRSWTAIYVSLDNVGMWNL 495

Query: 495 RSEFWARQYLGQQFYLRVYTPSTSLRDEYPIPKNALLCGRATGRRT 539
           RSE W RQYLGQQFY+RVYTPSTSLRDEY IPKNALLCGRATG  T
Sbjct: 496 RSELWERQYLGQQFYMRVYTPSTSLRDEYLIPKNALLCGRATGHHT 538

BLAST of MS016062 vs. TAIR 10
Match: AT1G21860.1 (SKU5 similar 7 )

HSP 1 Score: 822.0 bits (2122), Expect = 2.8e-238
Identity = 384/518 (74.13%), Postives = 441/518 (85.14%), Query Frame = 0

Query: 17  AIVAADDPYRFFNWNVTYGTIYPLGLPQQGILINGQFPGPDIHSVTNDNLIINVFNSLDE 76
           ++  A+DPYRFF W+VTYG I PLG+ QQGILING+FPGPDI S+TNDNLIINVFN LDE
Sbjct: 20  SLAFAEDPYRFFEWHVTYGNISPLGVAQQGILINGKFPGPDIISITNDNLIINVFNHLDE 79

Query: 77  PFLLSWNGIQQRRNSYEDGVYGTTCPIPPGKNFTYILQVKDQIGSFYYFPSLSFHKAAGG 136
           PFLLSWNGI+  +NS++DGVYGT CPIPPGKN+TY LQVKDQIGSFYYFPSL FHKAAGG
Sbjct: 80  PFLLSWNGIRNWKNSFQDGVYGTMCPIPPGKNYTYALQVKDQIGSFYYFPSLGFHKAAGG 139

Query: 137 FGGIRILSRPRIPVPFPDPAGDYTVLIGDWYQANHTTLRAQLDNGTMLPLPDGILINGRG 196
           FGGIRI SR  IPVPFP PA DYT+L+GDWY+ NH  L+AQLDNG  LPLPDGILINGR 
Sbjct: 140 FGGIRISSRALIPVPFPTPADDYTLLVGDWYKTNHKDLKAQLDNGGKLPLPDGILINGRS 199

Query: 197 PNRTAPFNVEQGKTYRLRISNVGLQNSLNFRIQGHRLKVVEVEGTHTLQTEFSSLDIHVG 256
              T   N+E GKTYRLRISNVGLQNSLNFRIQ H +K+VEVEG +T+Q  FSSLD+HVG
Sbjct: 200 SGAT--LNIEPGKTYRLRISNVGLQNSLNFRIQNHTMKLVEVEGRYTIQNLFSSLDVHVG 259

Query: 257 QSYSVLVTADQPAQDFYVVVSSRFTTPILTTTAILRYANSAGSVQGPPPGGPTIQIDWSL 316
           QSYSVL+TADQPA+D+YVVVSSRFT+ ILTTT +L Y+NS   V GP P GP I++ WS 
Sbjct: 260 QSYSVLITADQPAKDYYVVVSSRFTSKILTTTGVLHYSNSVAPVSGPIPDGP-IKLSWSF 319

Query: 317 NQARAIRTNLTASGPRPNPQGSYHYGLINTTKTIVLSNSAGFVNGKQRYAVNSVSFIPAD 376
           NQARAIRTNLTASGPRPNPQGSY YG+IN T+TI L+N+ G + GKQRYAVNS SF PAD
Sbjct: 320 NQARAIRTNLTASGPRPNPQGSYRYGVINITRTIRLANNLGHIEGKQRYAVNSASFYPAD 379

Query: 377 TPLKVADFFKIGGVFRVGSISDWPTGGGIYQDTSVMGADYRAYVEIVFQNNEDLIQSWHL 436
           TPLK+ D+FKI GV++ GSISD PT G I+  TSVM AD+RA+VE++F+N+ED++QSWHL
Sbjct: 380 TPLKLVDYFKIDGVYKPGSISDQPTNGAIFPTTSVMQADFRAFVEVIFENSEDIVQSWHL 439

Query: 437 DGYSFFVVGMDGGQWTSNSRNQYNLRDAIARCTTQVYPKSWTAIYVALDNVGMWNVRSEF 496
           DGYSF+VVGM+ G+W+  SR  YNL DAI RCT QVYP+SWTAIY+ALDNVGMWN+RSE 
Sbjct: 440 DGYSFYVVGMELGKWSPASRKVYNLNDAILRCTIQVYPRSWTAIYIALDNVGMWNMRSEI 499

Query: 497 WARQYLGQQFYLRVYTPSTSLRDEYPIPKNALLCGRAT 535
           W RQYLGQQFY+RVYT STSLRDEY IPKNALLCGRA+
Sbjct: 500 WERQYLGQQFYMRVYTTSTSLRDEYLIPKNALLCGRAS 534

BLAST of MS016062 vs. TAIR 10
Match: AT4G22010.1 (SKU5 similar 4 )

HSP 1 Score: 786.2 bits (2029), Expect = 1.7e-227
Identity = 366/523 (69.98%), Postives = 431/523 (82.41%), Query Frame = 0

Query: 19  VAADDPYRFFNWNVTYGTIYPLGLPQQGILINGQFPGPDIHSVTNDNLIINVFNSLDEPF 78
           V  D+PYRFF W +TYG IYPLG+ QQGILINGQFPGP I ++TNDN+II+VFN L EPF
Sbjct: 19  VLGDNPYRFFTWKITYGDIYPLGVKQQGILINGQFPGPHIDAITNDNIIISVFNYLKEPF 78

Query: 79  LLSWNGIQQRRNSYEDGVYGTTCPIPPGKNFTYILQVKDQIGSFYYFPSLSFHKAAGGFG 138
           L+SWNG+QQR+NS++DGV GTTCPIPPGKNFTY++QVKDQIGSFYYFPSL+FHKAAG FG
Sbjct: 79  LISWNGVQQRKNSWQDGVVGTTCPIPPGKNFTYVIQVKDQIGSFYYFPSLAFHKAAGAFG 138

Query: 139 GIRILSRPRIPVPFPDPAGDYTVLIGDWYQANHTTLRAQLDNGTMLPLPDGILINGRGPN 198
            IR+ SRPRIPVPF  P GD+ +L GDWY+ NH  LR  L+ G  LP PDG+LINGRG  
Sbjct: 139 AIRVWSRPRIPVPFSPPDGDFWLLAGDWYKTNHYVLRRLLEAGRNLPNPDGVLINGRGWG 198

Query: 199 RTAPFNVEQGKTYRLRISNVGLQNSLNFRIQGHRLKVVEVEGTHTLQTEFSSLDIHVGQS 258
               F V+ GKTYR RISNVG+  SLNFRIQGH +K+VEVEG+HT+Q  ++SLDIH+GQS
Sbjct: 199 GNT-FTVQPGKTYRFRISNVGVATSLNFRIQGHTMKLVEVEGSHTVQNIYTSLDIHLGQS 258

Query: 259 YSVLVTADQPAQDFYVVVSSRFTTPILTTTAILRYANSAGSVQGPPPGGPTIQIDWSLNQ 318
           YSVLVTA+Q  QD+Y+V+SSRFT  +LTTT+IL Y+NS   V GP P GPT+ I  SL Q
Sbjct: 259 YSVLVTANQAPQDYYIVISSRFTRKVLTTTSILHYSNSRKGVSGPVPNGPTLDIASSLYQ 318

Query: 319 ARAIRTNLTASGPRPNPQGSYHYGLINTTKTIVLSNSAGFVNGKQRYAVNSVSFIPADTP 378
           AR IR NLTASGPRPNPQGSYHYGLI   +TI+L+NSA ++NGKQRYAVN  SF+  DTP
Sbjct: 319 ARTIRRNLTASGPRPNPQGSYHYGLIKPGRTIILANSAPWINGKQRYAVNGASFVAPDTP 378

Query: 379 LKVADFFKIGGVFRVGSISDWPTGG-GIYQDTSVMGADYRAYVEIVFQNNEDLIQSWHLD 438
           LK+AD+FKI GVF +GSI   P+GG G Y  +SVM A++R ++E+VFQN E+ +QSWH+ 
Sbjct: 379 LKLADYFKIPGVFNLGSIPTSPSGGNGGYLQSSVMAANFREFIEVVFQNWENSVQSWHVS 438

Query: 439 GYSFFVVGMDGGQWTSNSRNQYNLRDAIARCTTQVYPKSWTAIYVALDNVGMWNVRSEFW 498
           GYSFFVVGMDGGQWT  SR +YNLRDA++R T QVYP++WTAIY+ALDNVGMWN+RSE W
Sbjct: 439 GYSFFVVGMDGGQWTPGSRAKYNLRDAVSRSTVQVYPRAWTAIYIALDNVGMWNIRSENW 498

Query: 499 ARQYLGQQFYLRVYTPSTSLRDEYPIPKNALLCGRATGRRTRP 541
           ARQYLGQQFYLRVYT STS RDEYP PKNAL+CGRA GR TRP
Sbjct: 499 ARQYLGQQFYLRVYTSSTSYRDEYPPPKNALMCGRAKGRHTRP 540