MS016061 (gene) Bitter gourd (TR) v1

BLAST of MS016061 vs. NCBI nr
Match: XP_022144357.1 (enoyl-CoA hydratase 2, peroxisomal [Momordica charantia])

HSP 1 Score: 578.9 bits (1491), Expect = 2.3e-161
Identity = 280/283 (98.94%), Postives = 282/283 (99.65%), Query Frame = 0

Query: 1   DRDVALYALGIGACCREAVDADELKYVYDENGQEYIEVLPTFAALFTLGTMERGLDLPGL 60
           +RDVALYALGIGACCREAVDADELKYVYDENGQEYIEVLPTFAALFTLGTMERGLDLPGL
Sbjct: 27  ERDVALYALGIGACCREAVDADELKYVYDENGQEYIEVLPTFAALFTLGTMERGLDLPGL 86

Query: 61  SYDPKLLLHGQQYIELYKPLPSSGNLDNKVSLAGLHDKGKAAILEIETKSYEKNSGQLLC 120
           SYDPKLLLHGQQYIELYKPLPSSGNLDNKVSLAGLHDKGKAAILEIETKSYEKNSGQLLC
Sbjct: 87  SYDPKLLLHGQQYIELYKPLPSSGNLDNKVSLAGLHDKGKAAILEIETKSYEKNSGQLLC 146

Query: 121 MNRTTLFLRGAGGFSNSSHPYSYTNYRKDQASVGRIPKTQPFAVYEDCTRPSQALLYRLS 180
           MNRTTLFLRGAGGFSNSSHPYSYTNYRKDQ SVGRIPKTQPFAVY+DCTRPSQALLYRLS
Sbjct: 147 MNRTTLFLRGAGGFSNSSHPYSYTNYRKDQESVGRIPKTQPFAVYKDCTRPSQALLYRLS 206

Query: 181 GDYNPLHSDPMIAKIAGFSKPILHGLCTLGFAVRAVIKCICKGDSRKVKCLSGKFLLHVY 240
           GDYNPLHSDPMIAKIAGFSKPILHGLCTLGFAVRAVIKCICKGDSRKVKCLSGKFLLHVY
Sbjct: 207 GDYNPLHSDPMIAKIAGFSKPILHGLCTLGFAVRAVIKCICKGDSRKVKCLSGKFLLHVY 266

Query: 241 PGETLVTEMWLDGSRVIYQTKVKERDRSVLSGYVDLHHATSSL 284
           PGETLVTEMWLDGSRVIYQTKVKERDRSVLSGYVDLHHATSSL
Sbjct: 267 PGETLVTEMWLDGSRVIYQTKVKERDRSVLSGYVDLHHATSSL 309

BLAST of MS016061 vs. NCBI nr
Match: XP_023547182.1 (enoyl-CoA hydratase 2, peroxisomal [Cucurbita pepo subsp. pepo])

HSP 1 Score: 523.1 bits (1346), Expect = 1.5e-144
Identity = 250/283 (88.34%), Postives = 267/283 (94.35%), Query Frame = 0

Query: 1   DRDVALYALGIGACCREAVDADELKYVYDENGQEYIEVLPTFAALFTLGTMERGLDLPGL 60
           +RDVALYALGIGAC REAVDADELKYVY+ENGQEYIEVLPTFAALF   TM  GL+LPGL
Sbjct: 27  ERDVALYALGIGACGREAVDADELKYVYNENGQEYIEVLPTFAALFIHKTMGTGLNLPGL 86

Query: 61  SYDPKLLLHGQQYIELYKPLPSSGNLDNKVSLAGLHDKGKAAILEIETKSYEKNSGQLLC 120
           SYDPKLLLHGQQYIELYKPLPS G LDNK+SLAGLHDKGKAAILEIETKSY+K SGQLLC
Sbjct: 87  SYDPKLLLHGQQYIELYKPLPSRGYLDNKISLAGLHDKGKAAILEIETKSYDKMSGQLLC 146

Query: 121 MNRTTLFLRGAGGFSNSSHPYSYTNYRKDQASVGRIPKTQPFAVYEDCTRPSQALLYRLS 180
           MNRTTLFLRGAGGFS+SSHPYSYTNY K+Q S G+IPKTQPF VYEDCTRPSQAL+YRLS
Sbjct: 147 MNRTTLFLRGAGGFSSSSHPYSYTNYPKNQGSAGKIPKTQPFMVYEDCTRPSQALVYRLS 206

Query: 181 GDYNPLHSDPMIAKIAGFSKPILHGLCTLGFAVRAVIKCICKGDSRKVKCLSGKFLLHVY 240
           GDYNPLHSDP++AKIAGFSKPILHGLCTLGFAVRAVIKC+CKGDS KVKC+SGKFLLHVY
Sbjct: 207 GDYNPLHSDPIVAKIAGFSKPILHGLCTLGFAVRAVIKCVCKGDSSKVKCISGKFLLHVY 266

Query: 241 PGETLVTEMWLDGSRVIYQTKVKERDRSVLSGYVDLHHATSSL 284
           PGETLVTEMWL+GSRV+YQTKVKER+R+VLSGYVDLHH  SSL
Sbjct: 267 PGETLVTEMWLEGSRVLYQTKVKERNRAVLSGYVDLHHVASSL 309

BLAST of MS016061 vs. NCBI nr
Match: XP_022961946.1 (enoyl-CoA hydratase 2, peroxisomal [Cucurbita moschata])

HSP 1 Score: 523.1 bits (1346), Expect = 1.5e-144
Identity = 250/283 (88.34%), Postives = 267/283 (94.35%), Query Frame = 0

Query: 1   DRDVALYALGIGACCREAVDADELKYVYDENGQEYIEVLPTFAALFTLGTMERGLDLPGL 60
           +RDVALYALGIGAC REAVDADELKYVY+ENGQEYIEVLPTFAALF   TM  GL+LPGL
Sbjct: 27  ERDVALYALGIGACGREAVDADELKYVYNENGQEYIEVLPTFAALFIHKTMGTGLNLPGL 86

Query: 61  SYDPKLLLHGQQYIELYKPLPSSGNLDNKVSLAGLHDKGKAAILEIETKSYEKNSGQLLC 120
           SYDPKLLLHGQQYIELYKPLPS G LDNK+SLAGLHDKGKAAILEIETKSY+K SGQLLC
Sbjct: 87  SYDPKLLLHGQQYIELYKPLPSRGYLDNKISLAGLHDKGKAAILEIETKSYDKMSGQLLC 146

Query: 121 MNRTTLFLRGAGGFSNSSHPYSYTNYRKDQASVGRIPKTQPFAVYEDCTRPSQALLYRLS 180
           MNRTTLFLRGAGGFS+SSHPYSYTNY K+Q S G+IPKTQPF VYEDCTRPSQAL+YRLS
Sbjct: 147 MNRTTLFLRGAGGFSSSSHPYSYTNYPKNQGSAGKIPKTQPFMVYEDCTRPSQALVYRLS 206

Query: 181 GDYNPLHSDPMIAKIAGFSKPILHGLCTLGFAVRAVIKCICKGDSRKVKCLSGKFLLHVY 240
           GDYNPLHSDP++AKIAGFSKPILHGLCTLGFAVRAVIKC+CKGDS KVKC+SGKFLLHVY
Sbjct: 207 GDYNPLHSDPIVAKIAGFSKPILHGLCTLGFAVRAVIKCVCKGDSSKVKCISGKFLLHVY 266

Query: 241 PGETLVTEMWLDGSRVIYQTKVKERDRSVLSGYVDLHHATSSL 284
           PGETLVTEMWL+GSRV+YQTKVKER+R+VLSGYVDLHH  SSL
Sbjct: 267 PGETLVTEMWLEGSRVLYQTKVKERNRAVLSGYVDLHHVASSL 309

BLAST of MS016061 vs. NCBI nr
Match: KAG7029461.1 (Enoyl-CoA hydratase 2, peroxisomal [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 522.3 bits (1344), Expect = 2.6e-144
Identity = 250/283 (88.34%), Postives = 266/283 (93.99%), Query Frame = 0

Query: 1   DRDVALYALGIGACCREAVDADELKYVYDENGQEYIEVLPTFAALFTLGTMERGLDLPGL 60
           +RDVALYALGIGAC REAVDADELKYVY+ENGQEYIEVLPTFAALF   TM  GL+LPGL
Sbjct: 27  ERDVALYALGIGACGREAVDADELKYVYNENGQEYIEVLPTFAALFIHKTMGTGLNLPGL 86

Query: 61  SYDPKLLLHGQQYIELYKPLPSSGNLDNKVSLAGLHDKGKAAILEIETKSYEKNSGQLLC 120
           SYDPKLLLHGQQYIELYKPLPS G LDNK+SLAGLHDKGKAAILEIETKSY+K SGQLLC
Sbjct: 87  SYDPKLLLHGQQYIELYKPLPSRGCLDNKISLAGLHDKGKAAILEIETKSYDKMSGQLLC 146

Query: 121 MNRTTLFLRGAGGFSNSSHPYSYTNYRKDQASVGRIPKTQPFAVYEDCTRPSQALLYRLS 180
           MNRTTLFLRGAGGFSNSSHPYSYTNY K+Q S G+IPKTQPF VYEDCTRPSQAL+YRLS
Sbjct: 147 MNRTTLFLRGAGGFSNSSHPYSYTNYPKNQGSAGKIPKTQPFMVYEDCTRPSQALVYRLS 206

Query: 181 GDYNPLHSDPMIAKIAGFSKPILHGLCTLGFAVRAVIKCICKGDSRKVKCLSGKFLLHVY 240
           GDYNPLHSDP++AKIAGFS PILHGLCTLGFAVRAVIKC+CKGDS KVKC+SGKFLLHVY
Sbjct: 207 GDYNPLHSDPIVAKIAGFSMPILHGLCTLGFAVRAVIKCVCKGDSSKVKCISGKFLLHVY 266

Query: 241 PGETLVTEMWLDGSRVIYQTKVKERDRSVLSGYVDLHHATSSL 284
           PGETLVTEMWL+GSRV+YQTKVKER+R+VLSGYVDLHH  SSL
Sbjct: 267 PGETLVTEMWLEGSRVLYQTKVKERNRAVLSGYVDLHHVASSL 309

BLAST of MS016061 vs. NCBI nr
Match: XP_022997336.1 (enoyl-CoA hydratase 2, peroxisomal [Cucurbita maxima])

HSP 1 Score: 520.0 bits (1338), Expect = 1.3e-143
Identity = 248/283 (87.63%), Postives = 266/283 (93.99%), Query Frame = 0

Query: 1   DRDVALYALGIGACCREAVDADELKYVYDENGQEYIEVLPTFAALFTLGTMERGLDLPGL 60
           +RDVALYALGIGAC REAVDADELKYVY+ENGQEYIEVLPTFAALF   TM  GL+LPGL
Sbjct: 27  ERDVALYALGIGACGREAVDADELKYVYNENGQEYIEVLPTFAALFIHKTMGTGLNLPGL 86

Query: 61  SYDPKLLLHGQQYIELYKPLPSSGNLDNKVSLAGLHDKGKAAILEIETKSYEKNSGQLLC 120
           SYDPKLLLHGQQYIELYKPLPS G LDNK+SLAGLHDKGKAAILEIETKSY+K SGQLLC
Sbjct: 87  SYDPKLLLHGQQYIELYKPLPSRGYLDNKISLAGLHDKGKAAILEIETKSYDKRSGQLLC 146

Query: 121 MNRTTLFLRGAGGFSNSSHPYSYTNYRKDQASVGRIPKTQPFAVYEDCTRPSQALLYRLS 180
           MNRTTLFLRGAGGFS+SSHPYSYTNY K+Q S G+IPKTQPF V+EDCTRPSQAL+YRLS
Sbjct: 147 MNRTTLFLRGAGGFSSSSHPYSYTNYPKNQGSAGKIPKTQPFMVFEDCTRPSQALVYRLS 206

Query: 181 GDYNPLHSDPMIAKIAGFSKPILHGLCTLGFAVRAVIKCICKGDSRKVKCLSGKFLLHVY 240
           GDYNPLHSDP++AKIAGFSKPILHGLCTLGFAVRAVIKC+CKGDS KVKC+SGKFLLHVY
Sbjct: 207 GDYNPLHSDPIVAKIAGFSKPILHGLCTLGFAVRAVIKCVCKGDSSKVKCISGKFLLHVY 266

Query: 241 PGETLVTEMWLDGSRVIYQTKVKERDRSVLSGYVDLHHATSSL 284
           PGE LVTEMWL+GSRV+YQTKVKER+R+VLSGYVDLHH  SSL
Sbjct: 267 PGEILVTEMWLEGSRVLYQTKVKERNRAVLSGYVDLHHVASSL 309

BLAST of MS016061 vs. ExPASy Swiss-Prot
Match: Q8VYI3 (Enoyl-CoA hydratase 2, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=ECH2 PE=1 SV=1)

HSP 1 Score: 433.0 bits (1112), Expect = 2.7e-120
Identity = 201/283 (71.02%), Postives = 243/283 (85.87%), Query Frame = 0

Query: 1   DRDVALYALGIGACCREAVDADELKYVYDENGQEYIEVLPTFAALFTLGTMERGLDLPGL 60
           +RDVA+YALGIGAC ++AVD+DELK+VY  NGQ+ I+VLPTFA+LFTLG++  GLDLPG 
Sbjct: 27  ERDVAIYALGIGACGQDAVDSDELKFVYHRNGQDLIQVLPTFASLFTLGSLTEGLDLPGF 86

Query: 61  SYDPKLLLHGQQYIELYKPLPSSGNLDNKVSLAGLHDKGKAAILEIETKSYEKNSGQLLC 120
            YDP LLLHGQQYIE+Y+PLPS  +L NKVSLAGL DKGKAAILE+ET+SYE+ SG+LLC
Sbjct: 87  KYDPSLLLHGQQYIEIYRPLPSKASLINKVSLAGLQDKGKAAILELETRSYEEGSGELLC 146

Query: 121 MNRTTLFLRGAGGFSNSSHPYSYTNYRKDQASVGRIPKTQPFAVYEDCTRPSQALLYRLS 180
           MNRTT+FLRGAGGFSNSS P+SY NY  +Q    +IP+ QP  V E+ T+PSQALLYRLS
Sbjct: 147 MNRTTVFLRGAGGFSNSSQPFSYKNYPSNQGLAVKIPQRQPLTVCEERTQPSQALLYRLS 206

Query: 181 GDYNPLHSDPMIAKIAGFSKPILHGLCTLGFAVRAVIKCICKGDSRKVKCLSGKFLLHVY 240
           GDYNPLHSDP  AK+AGF +PILHGLCTLGFA++A+IKC+CKGD   VK +SG+FL  V+
Sbjct: 207 GDYNPLHSDPEFAKLAGFPRPILHGLCTLGFAIKAIIKCVCKGDPTAVKTISGRFLTTVF 266

Query: 241 PGETLVTEMWLDGSRVIYQTKVKERDRSVLSGYVDLHHATSSL 284
           PGETL+TEMWL+G RVIYQTKVKER+++VL+GYVD+   +SSL
Sbjct: 267 PGETLITEMWLEGLRVIYQTKVKERNKTVLAGYVDIRGLSSSL 309

BLAST of MS016061 vs. ExPASy Swiss-Prot
Match: P51659 (Peroxisomal multifunctional enzyme type 2 OS=Homo sapiens OX=9606 GN=HSD17B4 PE=1 SV=3)

HSP 1 Score: 216.1 bits (549), Expect = 5.2e-55
Identity = 118/278 (42.45%), Postives = 165/278 (59.35%), Query Frame = 0

Query: 3   DVALYALGIGACCREAVDADELKYVYDENGQEYIEVLPTFAALFTLGTMERG--LDLPGL 62
           +  +YALG+GA  +   D  +LK++Y+  G      LPTF  +    +M  G   ++PGL
Sbjct: 343 EAIMYALGVGASIK---DPKDLKFIYE--GSSDFSCLPTFGVIIGQKSMMGGGLAEIPGL 402

Query: 63  SYDPKLLLHGQQYIELYKPLPSSGNLDNKVSLAGLHDKGKAAILEIETKSYEKNSGQLLC 122
           S +   +LHG+QY+ELYKPLP +G L  +  +A + DKG   ++ ++  SY +   +L+C
Sbjct: 403 SINFAKVLHGEQYLELYKPLPRAGKLKCEAVVADVLDKGSGVVIIMDVYSYSEK--ELIC 462

Query: 123 MNRTTLFLRGAGGFSNSSHPYSYTNYRKDQASVG-RIPKTQPFAVYEDCTRPSQALLYRL 182
            N+ +LFL G+GGF              D+  V   IP   P AV  D T  +QA LYRL
Sbjct: 463 HNQFSLFLVGSGGFGGKR--------TSDKVKVAVAIPNRPPDAVLTDTTSLNQAALYRL 522

Query: 183 SGDYNPLHSDPMIAKIAGFSKPILHGLCTLGFAVRAVIKCICKGDSRKVKCLSGKFLLHV 242
           SGD+NPLH DP  A +AGF KPILHGLCT GF+ R V++     D  + K +  +F   V
Sbjct: 523 SGDWNPLHIDPNFASLAGFDKPILHGLCTFGFSARRVLQQFADNDVSRFKAIKARFAKPV 582

Query: 243 YPGETLVTEMWLDGSRVIYQTKVKERDRSVLS-GYVDL 277
           YPG+TL TEMW +G+R+ +QTKV+E    V+S  YVDL
Sbjct: 583 YPGQTLQTEMWKEGNRIHFQTKVQETGDIVISNAYVDL 605

BLAST of MS016061 vs. ExPASy Swiss-Prot
Match: P51660 (Peroxisomal multifunctional enzyme type 2 OS=Mus musculus OX=10090 GN=Hsd17b4 PE=1 SV=3)

HSP 1 Score: 214.2 bits (544), Expect = 2.0e-54
Identity = 118/274 (43.07%), Postives = 164/274 (59.85%), Query Frame = 0

Query: 6   LYALGIGACCREAVDADELKYVYDENGQEYIEVLPTFAALFTLGTMERG--LDLPGLSYD 65
           +YALG+GA  +   D   LK+VY+  G      LPTF  +    +M  G   ++PGLS++
Sbjct: 345 MYALGVGASVKNPKD---LKFVYE--GSADFSCLPTFGVIVAQKSMMNGGLAEVPGLSFN 404

Query: 66  PKLLLHGQQYIELYKPLPSSGNLDNKVSLAGLHDKGKAAILEIETKSYEKNSGQLLCMNR 125
               LHG+QY+ELYKPLP SG L  +  +A + DKG   ++ ++  SY  +  +L+C N+
Sbjct: 405 FAKALHGEQYLELYKPLPRSGELKCEAVIADILDKGSGVVIVMDVYSY--SGKELICYNQ 464

Query: 126 TTLFLRGAGGFSNSSHPYSYTNYRKDQASVGRIPKTQPFAVYEDCTRPSQALLYRLSGDY 185
            ++F+ G+GGF             K +A+V  +P   P AV  D T  +QA LYRLSGD+
Sbjct: 465 FSVFVVGSGGFGGK------RTSEKLKAAVA-VPNRPPDAVLRDATSLNQAALYRLSGDW 524

Query: 186 NPLHSDPMIAKIAGFSKPILHGLCTLGFAVRAVIKCICKGDSRKVKCLSGKFLLHVYPGE 245
           NPLH DP  A +AGF KPILHGLCT GF+ R V++     D  + K +  +F   VYPG+
Sbjct: 525 NPLHIDPDFASVAGFEKPILHGLCTFGFSARHVLQQFADNDVSRFKAIKVRFAKPVYPGQ 584

Query: 246 TLVTEMWLDGSRVIYQTKVKERDRSVLS-GYVDL 277
           TL TEMW +G+R+ +QTKV E    V+S  YVDL
Sbjct: 585 TLQTEMWKEGNRIHFQTKVHETGDVVISNAYVDL 604

BLAST of MS016061 vs. ExPASy Swiss-Prot
Match: P97852 (Peroxisomal multifunctional enzyme type 2 OS=Rattus norvegicus OX=10116 GN=Hsd17b4 PE=1 SV=3)

HSP 1 Score: 211.5 bits (537), Expect = 1.3e-53
Identity = 118/274 (43.07%), Postives = 164/274 (59.85%), Query Frame = 0

Query: 6   LYALGIGACCREAVDADELKYVYDENGQEYIEVLPTFAALFTLGTMERG--LDLPGLSYD 65
           +YALG+GA  +   D   LK+VY+  G      LPTF  +    ++  G   ++PGLS +
Sbjct: 345 MYALGVGASVKNPKD---LKFVYE--GSADFSCLPTFGVIVAQKSLMSGGLAEVPGLSIN 404

Query: 66  PKLLLHGQQYIELYKPLPSSGNLDNKVSLAGLHDKGKAAILEIETKSYEKNSGQLLCMNR 125
              +LHG+QY+ELYKPLP SG L  +  +A + DKG   ++ ++  SY  +  +L+C N+
Sbjct: 405 FAKVLHGEQYLELYKPLPRSGELKCEAVIADILDKGSGIVIVMDVYSY--SGKELICYNQ 464

Query: 126 TTLFLRGAGGFSNSSHPYSYTNYRKDQASVGRIPKTQPFAVYEDCTRPSQALLYRLSGDY 185
            ++F+ G+GGF             K +A+V  +P   P AV  D T  +QA LYRLSGD 
Sbjct: 465 FSVFVVGSGGFGGK------RTSEKLKAAVA-VPSRPPDAVLRDTTSLNQAALYRLSGDS 524

Query: 186 NPLHSDPMIAKIAGFSKPILHGLCTLGFAVRAVIKCICKGDSRKVKCLSGKFLLHVYPGE 245
           NPLH DP  A IAGF KPILHGLCT GF+ R V++     D  + K +  +F   VYPG+
Sbjct: 525 NPLHIDPSFASIAGFEKPILHGLCTFGFSARHVLQQFADNDVSRFKAIKVRFAKPVYPGQ 584

Query: 246 TLVTEMWLDGSRVIYQTKVKERDRSVLS-GYVDL 277
           TL TEMW +G+R+ +QTKV+E    V+S  YVDL
Sbjct: 585 TLQTEMWKEGNRIHFQTKVQETGDIVISNAYVDL 604

BLAST of MS016061 vs. ExPASy Swiss-Prot
Match: Q01373 (Peroxisomal hydratase-dehydrogenase-epimerase OS=Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) OX=367110 GN=fox-2 PE=1 SV=1)

HSP 1 Score: 201.8 bits (512), Expect = 1.0e-50
Identity = 117/274 (42.70%), Postives = 159/274 (58.03%), Query Frame = 0

Query: 1   DRDVALYALGIGACCREAVDADELKYVYDENGQEYIEVLPTFAALFTLGT-MERGLDLPG 60
           +RDV LY LGIGA         ++KY+++  G E  EV+PTF  +    T M    D   
Sbjct: 632 ERDVCLYNLGIGA------KRTDIKYIFE--GNEDFEVVPTFGVIPPFNTEMPFSFDDIV 691

Query: 61  LSYDPKLLLHGQQYIELYK-PLPSSGNLDNKVSLAGLHDKGKAAILEIETKSYEKNSGQL 120
            ++ P +LLHG+QY+E+ K P+P+SG L +K  L  + DKG AAI++    ++   +G+ 
Sbjct: 692 PNFSPMMLLHGEQYLEVRKYPIPTSGRLVSKGKLLEVVDKGSAAIVKQGITTFNAETGEE 751

Query: 121 LCMNRTTLFLRGAGGFSNSSHPYSYTNYRKDQASVGRIPKTQPFAVYEDCTRPSQALLYR 180
           L  N  T+FLRG GGF     P      R    +  + P   P AV E  T   QA +YR
Sbjct: 752 LFYNEMTVFLRGCGGFGGQKKPAD----RGASTAANKPPARSPDAVVEVQTTEEQAAIYR 811

Query: 181 LSGDYNPLHSDPMIAKIAGFSKPILHGLCTLGFAVRAVIKCICKGDSRKVKCLSGKFLLH 240
           LSGDYNPLH DP  AK+ GF  PILHGLC+ G A +AV +   K  + KV     +F   
Sbjct: 812 LSGDYNPLHVDPAFAKVGGFKVPILHGLCSFGIAGKAVYEKYGKFKNIKV-----RFAGT 871

Query: 241 VYPGETLVTEMWLDGSRVIYQTKVKERDRSVLSG 273
           V PG+TLVTEMW +G++V++QTKVKE  +  +SG
Sbjct: 872 VNPGQTLVTEMWKEGNKVVFQTKVKETGKLAISG 888

BLAST of MS016061 vs. ExPASy TrEMBL
Match: A0A6J1CTG7 (enoyl-CoA hydratase 2, peroxisomal OS=Momordica charantia OX=3673 GN=LOC111014062 PE=4 SV=1)

HSP 1 Score: 578.9 bits (1491), Expect = 1.1e-161
Identity = 280/283 (98.94%), Postives = 282/283 (99.65%), Query Frame = 0

Query: 1   DRDVALYALGIGACCREAVDADELKYVYDENGQEYIEVLPTFAALFTLGTMERGLDLPGL 60
           +RDVALYALGIGACCREAVDADELKYVYDENGQEYIEVLPTFAALFTLGTMERGLDLPGL
Sbjct: 27  ERDVALYALGIGACCREAVDADELKYVYDENGQEYIEVLPTFAALFTLGTMERGLDLPGL 86

Query: 61  SYDPKLLLHGQQYIELYKPLPSSGNLDNKVSLAGLHDKGKAAILEIETKSYEKNSGQLLC 120
           SYDPKLLLHGQQYIELYKPLPSSGNLDNKVSLAGLHDKGKAAILEIETKSYEKNSGQLLC
Sbjct: 87  SYDPKLLLHGQQYIELYKPLPSSGNLDNKVSLAGLHDKGKAAILEIETKSYEKNSGQLLC 146

Query: 121 MNRTTLFLRGAGGFSNSSHPYSYTNYRKDQASVGRIPKTQPFAVYEDCTRPSQALLYRLS 180
           MNRTTLFLRGAGGFSNSSHPYSYTNYRKDQ SVGRIPKTQPFAVY+DCTRPSQALLYRLS
Sbjct: 147 MNRTTLFLRGAGGFSNSSHPYSYTNYRKDQESVGRIPKTQPFAVYKDCTRPSQALLYRLS 206

Query: 181 GDYNPLHSDPMIAKIAGFSKPILHGLCTLGFAVRAVIKCICKGDSRKVKCLSGKFLLHVY 240
           GDYNPLHSDPMIAKIAGFSKPILHGLCTLGFAVRAVIKCICKGDSRKVKCLSGKFLLHVY
Sbjct: 207 GDYNPLHSDPMIAKIAGFSKPILHGLCTLGFAVRAVIKCICKGDSRKVKCLSGKFLLHVY 266

Query: 241 PGETLVTEMWLDGSRVIYQTKVKERDRSVLSGYVDLHHATSSL 284
           PGETLVTEMWLDGSRVIYQTKVKERDRSVLSGYVDLHHATSSL
Sbjct: 267 PGETLVTEMWLDGSRVIYQTKVKERDRSVLSGYVDLHHATSSL 309

BLAST of MS016061 vs. ExPASy TrEMBL
Match: A0A6J1HBS0 (enoyl-CoA hydratase 2, peroxisomal OS=Cucurbita moschata OX=3662 GN=LOC111462566 PE=4 SV=1)

HSP 1 Score: 523.1 bits (1346), Expect = 7.3e-145
Identity = 250/283 (88.34%), Postives = 267/283 (94.35%), Query Frame = 0

Query: 1   DRDVALYALGIGACCREAVDADELKYVYDENGQEYIEVLPTFAALFTLGTMERGLDLPGL 60
           +RDVALYALGIGAC REAVDADELKYVY+ENGQEYIEVLPTFAALF   TM  GL+LPGL
Sbjct: 27  ERDVALYALGIGACGREAVDADELKYVYNENGQEYIEVLPTFAALFIHKTMGTGLNLPGL 86

Query: 61  SYDPKLLLHGQQYIELYKPLPSSGNLDNKVSLAGLHDKGKAAILEIETKSYEKNSGQLLC 120
           SYDPKLLLHGQQYIELYKPLPS G LDNK+SLAGLHDKGKAAILEIETKSY+K SGQLLC
Sbjct: 87  SYDPKLLLHGQQYIELYKPLPSRGYLDNKISLAGLHDKGKAAILEIETKSYDKMSGQLLC 146

Query: 121 MNRTTLFLRGAGGFSNSSHPYSYTNYRKDQASVGRIPKTQPFAVYEDCTRPSQALLYRLS 180
           MNRTTLFLRGAGGFS+SSHPYSYTNY K+Q S G+IPKTQPF VYEDCTRPSQAL+YRLS
Sbjct: 147 MNRTTLFLRGAGGFSSSSHPYSYTNYPKNQGSAGKIPKTQPFMVYEDCTRPSQALVYRLS 206

Query: 181 GDYNPLHSDPMIAKIAGFSKPILHGLCTLGFAVRAVIKCICKGDSRKVKCLSGKFLLHVY 240
           GDYNPLHSDP++AKIAGFSKPILHGLCTLGFAVRAVIKC+CKGDS KVKC+SGKFLLHVY
Sbjct: 207 GDYNPLHSDPIVAKIAGFSKPILHGLCTLGFAVRAVIKCVCKGDSSKVKCISGKFLLHVY 266

Query: 241 PGETLVTEMWLDGSRVIYQTKVKERDRSVLSGYVDLHHATSSL 284
           PGETLVTEMWL+GSRV+YQTKVKER+R+VLSGYVDLHH  SSL
Sbjct: 267 PGETLVTEMWLEGSRVLYQTKVKERNRAVLSGYVDLHHVASSL 309

BLAST of MS016061 vs. ExPASy TrEMBL
Match: A0A6J1K779 (enoyl-CoA hydratase 2, peroxisomal OS=Cucurbita maxima OX=3661 GN=LOC111492280 PE=4 SV=1)

HSP 1 Score: 520.0 bits (1338), Expect = 6.2e-144
Identity = 248/283 (87.63%), Postives = 266/283 (93.99%), Query Frame = 0

Query: 1   DRDVALYALGIGACCREAVDADELKYVYDENGQEYIEVLPTFAALFTLGTMERGLDLPGL 60
           +RDVALYALGIGAC REAVDADELKYVY+ENGQEYIEVLPTFAALF   TM  GL+LPGL
Sbjct: 27  ERDVALYALGIGACGREAVDADELKYVYNENGQEYIEVLPTFAALFIHKTMGTGLNLPGL 86

Query: 61  SYDPKLLLHGQQYIELYKPLPSSGNLDNKVSLAGLHDKGKAAILEIETKSYEKNSGQLLC 120
           SYDPKLLLHGQQYIELYKPLPS G LDNK+SLAGLHDKGKAAILEIETKSY+K SGQLLC
Sbjct: 87  SYDPKLLLHGQQYIELYKPLPSRGYLDNKISLAGLHDKGKAAILEIETKSYDKRSGQLLC 146

Query: 121 MNRTTLFLRGAGGFSNSSHPYSYTNYRKDQASVGRIPKTQPFAVYEDCTRPSQALLYRLS 180
           MNRTTLFLRGAGGFS+SSHPYSYTNY K+Q S G+IPKTQPF V+EDCTRPSQAL+YRLS
Sbjct: 147 MNRTTLFLRGAGGFSSSSHPYSYTNYPKNQGSAGKIPKTQPFMVFEDCTRPSQALVYRLS 206

Query: 181 GDYNPLHSDPMIAKIAGFSKPILHGLCTLGFAVRAVIKCICKGDSRKVKCLSGKFLLHVY 240
           GDYNPLHSDP++AKIAGFSKPILHGLCTLGFAVRAVIKC+CKGDS KVKC+SGKFLLHVY
Sbjct: 207 GDYNPLHSDPIVAKIAGFSKPILHGLCTLGFAVRAVIKCVCKGDSSKVKCISGKFLLHVY 266

Query: 241 PGETLVTEMWLDGSRVIYQTKVKERDRSVLSGYVDLHHATSSL 284
           PGE LVTEMWL+GSRV+YQTKVKER+R+VLSGYVDLHH  SSL
Sbjct: 267 PGEILVTEMWLEGSRVLYQTKVKERNRAVLSGYVDLHHVASSL 309

BLAST of MS016061 vs. ExPASy TrEMBL
Match: A0A0A0LQJ5 (MaoC-like domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G348190 PE=4 SV=1)

HSP 1 Score: 509.6 bits (1311), Expect = 8.4e-141
Identity = 240/283 (84.81%), Postives = 265/283 (93.64%), Query Frame = 0

Query: 1   DRDVALYALGIGACCREAVDADELKYVYDENGQEYIEVLPTFAALFTLGTMERGLDLPGL 60
           +RDVALYALG+GAC ++AVDADELKYVY+ENGQEYIEVLPTF+ALF + T+  GL+LPGL
Sbjct: 27  ERDVALYALGVGACGQQAVDADELKYVYNENGQEYIEVLPTFSALFIIDTLSTGLNLPGL 86

Query: 61  SYDPKLLLHGQQYIELYKPLPSSGNLDNKVSLAGLHDKGKAAILEIETKSYEKNSGQLLC 120
            YDPKLLLHGQQYIELYKPL SSG+LDNK+SLAGLHDKGKAAILEI TKSY+K SG LLC
Sbjct: 87  LYDPKLLLHGQQYIELYKPLSSSGHLDNKISLAGLHDKGKAAILEIATKSYDKKSGHLLC 146

Query: 121 MNRTTLFLRGAGGFSNSSHPYSYTNYRKDQASVGRIPKTQPFAVYEDCTRPSQALLYRLS 180
           MNRTTLFLRGAGGFS+SS+P+SYTNY KD+ S G+IPKTQPF VYEDCTRPSQALLYRLS
Sbjct: 147 MNRTTLFLRGAGGFSSSSNPFSYTNYPKDEGSAGKIPKTQPFTVYEDCTRPSQALLYRLS 206

Query: 181 GDYNPLHSDPMIAKIAGFSKPILHGLCTLGFAVRAVIKCICKGDSRKVKCLSGKFLLHVY 240
           GDYNPLHSDPM+AKIAGFSKPILHGLCTLGFAVRAVIKC+CKGDS KVKC+ GKFLLHVY
Sbjct: 207 GDYNPLHSDPMVAKIAGFSKPILHGLCTLGFAVRAVIKCVCKGDSSKVKCILGKFLLHVY 266

Query: 241 PGETLVTEMWLDGSRVIYQTKVKERDRSVLSGYVDLHHATSSL 284
           PGETLVTEMWL+GSRV+YQTKVKER+R+VLSGYVDL+H  SSL
Sbjct: 267 PGETLVTEMWLEGSRVLYQTKVKERNRAVLSGYVDLNHVASSL 309

BLAST of MS016061 vs. ExPASy TrEMBL
Match: A0A1S3BD85 (enoyl-CoA hydratase 2, peroxisomal OS=Cucumis melo OX=3656 GN=LOC103488374 PE=4 SV=1)

HSP 1 Score: 509.6 bits (1311), Expect = 8.4e-141
Identity = 242/283 (85.51%), Postives = 265/283 (93.64%), Query Frame = 0

Query: 1   DRDVALYALGIGACCREAVDADELKYVYDENGQEYIEVLPTFAALFTLGTMERGLDLPGL 60
           +RDVALYALG+GAC ++AVDADELKYVY+ENGQEYIEVLPTF+ALF L T+  GL+LPGL
Sbjct: 27  ERDVALYALGVGACGQQAVDADELKYVYNENGQEYIEVLPTFSALFILDTLSTGLNLPGL 86

Query: 61  SYDPKLLLHGQQYIELYKPLPSSGNLDNKVSLAGLHDKGKAAILEIETKSYEKNSGQLLC 120
           SYDPKLLLHGQQYIELYKPL SSG LDNKVSLAGLHDKGKAAILE+ TKSY+K SGQLLC
Sbjct: 87  SYDPKLLLHGQQYIELYKPLSSSGYLDNKVSLAGLHDKGKAAILEVATKSYDKKSGQLLC 146

Query: 121 MNRTTLFLRGAGGFSNSSHPYSYTNYRKDQASVGRIPKTQPFAVYEDCTRPSQALLYRLS 180
           MNRTTLFLRGAGGFS+SS+P+SYTNY KDQ S G+IPKTQPF VYEDCTRPSQALLYRLS
Sbjct: 147 MNRTTLFLRGAGGFSSSSNPFSYTNYPKDQGSAGKIPKTQPFTVYEDCTRPSQALLYRLS 206

Query: 181 GDYNPLHSDPMIAKIAGFSKPILHGLCTLGFAVRAVIKCICKGDSRKVKCLSGKFLLHVY 240
           GDYNPLHSDPM+AK+AGFSKPILHGLCTLGFAVRAVIKC+CKGDS KVK + GKFLLHVY
Sbjct: 207 GDYNPLHSDPMVAKVAGFSKPILHGLCTLGFAVRAVIKCVCKGDSSKVKRILGKFLLHVY 266

Query: 241 PGETLVTEMWLDGSRVIYQTKVKERDRSVLSGYVDLHHATSSL 284
           PGETLVTEMWL+GSRV+YQTKVKER+R+VLSGYVDL+H  SSL
Sbjct: 267 PGETLVTEMWLEGSRVLYQTKVKERNRAVLSGYVDLNHVASSL 309

BLAST of MS016061 vs. TAIR 10
Match: AT1G76150.1 (enoyl-CoA hydratase 2 )

HSP 1 Score: 433.0 bits (1112), Expect = 1.9e-121
Identity = 201/283 (71.02%), Postives = 243/283 (85.87%), Query Frame = 0

Query: 1   DRDVALYALGIGACCREAVDADELKYVYDENGQEYIEVLPTFAALFTLGTMERGLDLPGL 60
           +RDVA+YALGIGAC ++AVD+DELK+VY  NGQ+ I+VLPTFA+LFTLG++  GLDLPG 
Sbjct: 27  ERDVAIYALGIGACGQDAVDSDELKFVYHRNGQDLIQVLPTFASLFTLGSLTEGLDLPGF 86

Query: 61  SYDPKLLLHGQQYIELYKPLPSSGNLDNKVSLAGLHDKGKAAILEIETKSYEKNSGQLLC 120
            YDP LLLHGQQYIE+Y+PLPS  +L NKVSLAGL DKGKAAILE+ET+SYE+ SG+LLC
Sbjct: 87  KYDPSLLLHGQQYIEIYRPLPSKASLINKVSLAGLQDKGKAAILELETRSYEEGSGELLC 146

Query: 121 MNRTTLFLRGAGGFSNSSHPYSYTNYRKDQASVGRIPKTQPFAVYEDCTRPSQALLYRLS 180
           MNRTT+FLRGAGGFSNSS P+SY NY  +Q    +IP+ QP  V E+ T+PSQALLYRLS
Sbjct: 147 MNRTTVFLRGAGGFSNSSQPFSYKNYPSNQGLAVKIPQRQPLTVCEERTQPSQALLYRLS 206

Query: 181 GDYNPLHSDPMIAKIAGFSKPILHGLCTLGFAVRAVIKCICKGDSRKVKCLSGKFLLHVY 240
           GDYNPLHSDP  AK+AGF +PILHGLCTLGFA++A+IKC+CKGD   VK +SG+FL  V+
Sbjct: 207 GDYNPLHSDPEFAKLAGFPRPILHGLCTLGFAIKAIIKCVCKGDPTAVKTISGRFLTTVF 266

Query: 241 PGETLVTEMWLDGSRVIYQTKVKERDRSVLSGYVDLHHATSSL 284
           PGETL+TEMWL+G RVIYQTKVKER+++VL+GYVD+   +SSL
Sbjct: 267 PGETLITEMWLEGLRVIYQTKVKERNKTVLAGYVDIRGLSSSL 309