MS015815 (gene) Bitter gourd (TR) v1

Overview


Name	MS015815
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Histidine--tRNA ligase
Location	scaffold943_2: 136568 .. 141343 (-)
RNA-Seq Expression	MS015815
Synteny	MS015815

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTGCGAAGCCTGCGTCTCTCCTTATCTTCCGATCCCGCCTCAATCTATACTTCAAGCCCTTCATTTTCTCTTCTTCATCCCTCGCTTTCACTATCACTCTCAGATTTCCCTTTCCGAAGCCCTCATTCTTCGCCAATCGCACGTTTCATTCTTCTCTCAGCGCCGTCTCTTCCGCCCAGTCTCTCGGCGACAGCGGCAGCGCTGGCGGCAGGTCGGGGGCGCTTTCTCCATCGCCGGTCACGGAAGAACTGCAGAAGATCGATGTCAACCCTCCTAAGGGAACTCGAGATTTCCCACCCGAAGATATGCGACTCCGCAACTGGCTATTCCATAATTTCAGGGAGGTTTGTTGTCCTGTTTGGTTGAATTCTAGGAAGGTTCGTTGTTCTTTTGTGTCTCTGAGCTCTGGTTTCTGTTGTTTAATAGGTTTCGCGAGTCTACGGGTTTGAGGAGATTGATTTTCCGGTGCTCGAGTCGGAGGCGCTGTTCATTAGGAAAGCGGGTGAAGAAATAAGAGACCAGGTTGGTGTTGGTGTAATTGTTTGAGTTGGGTTCCGCCTGTTTACTGATTTTGTAGTTTTGGACTAATTTGTTAAATTGAGTGAAATTAAAAAGGAAAAATCATGATTCTGCAAATAATTTTGGCTGTATCTTATAATTTACTCTCAAACTGTTAGTATGAGTATCTGCCTTTAATTTCCTGCTCGAATTCAGTTGCACTCAGTAGCAGGACTTCGCATGAATAGGCAAGTGATTGATATTCAGTGATAGAGGGGCTGATTTTCCATCTGCCATTTTGGGATATGGGAGTGATTCTCTAGCATGCAACTTTCTTTGATATTTTGTAGAACATCTATAGGTAGGGAGAAATAGAAGAATTTCTAGAAGGCTTGGAAGTCTGAAGTATGGTTTGAGTTACATTACATCCTTTGGGTGAGAATGTCTAAGGATTTTTATAACTTATATTTATGTCTTATTTTCAACCATTGTAGCCAATCTCTTATTCTCAACTTGTATTGGCTCCTTTAATTGTCGTTTTTTTATGCTCGGTTGTTTTCTTTCATTTGCTTCAATGAAAGCTTGGTTTTTTCATAAAGGAAATTAAAAGATATTAATACCACAGCAGATAGGAAAGTTGTACAAGAAATGAGACAATTTAAAGAGCCAAATGGAATCCTGATATCTAATTCTTACTCGTTGACTGACTAAAGATGAAAAATAAGAGTCTTTTCCATTTTAAGCAATTTGTGCATCAGTTTTTGTTTTTTTTTATTGAAGCTATATTTGTGTAGAAGTTTAAGGTGGTAACCAACTTTTGCGATATACCAACCCAATTTGTATTAGCGATGAAATTTTTATGGTAACCATTTTATCATTTTTTAACTTGTTTCCTTACCTATCCTTTACGGTTTTAGAGCTGACAACTACAAGTAATCCACAATATACTGTTTTGGTGTTTCACTTTATTCACATTTCAAATTGTTTGGGGCTTTTATTATGGGTACATCTGCCTGCTTCTTCTTCTTCTTTTATGTTTTTCTCCCCTTATATGTTGCTACTTGAAATTGTAACATTTTGGCCAAAGAATATTGTAATAAAACCTCAAATAATGTGGAAACTTTATGGCTTGTATTAGTGATTTTATTTTGTGCTTTTTGTTTATGTAGCTCTATTGTTTTGAAGATCGGGGAAATCGTCGAGTTGCTTTACGGCCTGAGCTTACCCCTTCTTTGGCAAGATTAGTGATCCAGAAAGGGTACTTTGATTAATCTCAAAATGTGTTTTCTGTATTACATGATTTTTTATATTGGGTCTGTGAAAATTTAATCTGTCTATTTATTTATTTTTAATTATCTTTGTATCTAGGTACAGAGGCCAGAATTTGATCTCCATCTTTTTTCCTGTCCAGAAAATCTCTGTCCCTCCCATTGAAATGGTTTGCTATAGGACAATGCTGGCGATATGAGAGAATGACGAGAGGTCGGCGTCGTGAGCATTACCAATGGAATATGGATATCATTGGTGTACCCGAGGTTACGGTATGTGAAAAGTTTTAAACTTGCTTGTGGAACTGTATCTGATTTAGTATTTCTTTGCTAGTTAGAAATAGCTCATTTTTGCAATATAAGAAATCAATAATCATGCAACTTTCTATACCTTCGTACGTAGTCTTAAGAATAGTTTATGCTAGAAAACTTCCTCTCTCCTCATGTCATATGTCCCATTTCATCTTCGTGCTGTGGAAACTGAAAAAACGAATTTAGTTCACTATTAAAGGTTCATCTCGGGCATGGTCTGATAGTAATTTAGAATACCCTTTGGTATCTGCCTTGAATTAATATTGAAACTTGTTCCTGGTATACAAATACTCATGTGTGCCCATTTTATTTATACTTATATTTTAACTTCTTTGTACTCTCTAGGCTGAAGCAGAGCTTCTATCCTCCATTGTTACTTTTTTCAAGCGAATTGGAATTACAGCAGCTGATGTTGGATTTAAGGTTTCCAGCCGAAAGGTATCTTTTATGTTATGCATGTTGTGTGATGGTTACAATAGATGCAGTTTATAGCTTATAGGCTTTCGTAGTTATTAAGAGTTCAATAGTGAACCAAAGAGTTCAATAGTTATAGTTGGAGTTAATCCCCAATTTTTTTTGTTTGATGGTAGGTTTTGCAAGAAGTATTAGAATGTTTTGGAATACCTGAAAATATGTTTGGCAAGGTTTGCATTATCATAGACAAGGTTAGTGATCTAAGATTCAAGTTCCACAAATTTAGTTTCAATGTTTCTGTGCTTGTTTCCTAGCATGTTTGGATGCAAAAATTAATTACCTCTGTCTCTCTCTGTGTTTTCCCGCTGGCTATTATTGTTAAGAACAGATAGAGAAAATTCCATTAGATGAGATGAAGAAGGAACTGAAAGCTGTGGGTGTATCAGAGGAGGCTATTGAGGAGCTGTTGCAAGTTCTTTCAGTAAAGTCTTTGACAAAGCTAGAAGGTTATTTCCTACATAAAATGGTGCCTTGAGGAATCGGTTTATGTTTTCTTGAATGAAATTTTCAAAATAAATATTTTCATGTTGATAGAGTCTCTCTACAAATAACTTGGAGTGGATGTGATAACTCTAATTGTGGCATTGAAATTGATTGAAGTGTGATTGTTATTCTTGCATAGAGATAATTGGAGGTGGTGGGGAAGCGGTGTCTGATCTGAAGCAGCTGTTTTCTCTCGCTGAAAAGTTTGGGTACGCTGATTGGATCCAATTCGACGCCTCCATTGTCCGCGGCCTTGCTTATTATACTGGGATTGTTTTTGAGGTTAGTTTCGTCGTTAACATCCGATAATTGATACTAACTCCTCCCAAGAACTTGTTTGTCTCGATGATTTCATTTCATAGGTTTAACATATATTGTCATTTACTGAAATAATGCCACGTCAACGCTCTAATCTACTGTCAATACATCTAAAATTAAGTTGAACTTATTTCAGAAAGTAATGGAATAAATGTGGGAAAAGCAGCTTCATTAATGGCCTAAAGTCCTAAATCTAGAAGTATAATATAATGTTATGATGTAAAATCTTTAATAATAGAAAGTTTGTAAACACACCCTTGTTTTACCTCCTTTCTTCAGTCAATATTTGTTCTTAAGCAGCTATGTCTCCATATGTATAGAATAGAAGTTATAGTTAATTAGCTATGGCTATTTCTTGCGAATTGATTTATAGGAAAAATTGGTAAACTGATCAAAATTGTATTCTTCTCTCTTGTCCTCGTGTGAGTTCAATTAAAGAAAAGTCTAATGTCATGACCATTGTTCTTTTCTTTTTTAAAAATAATAATTATATTATTCTTCTTTTCATAAATAATTTTGGTGGTATGAACGAATCAGGGATTTGATAGAGGAGGAAAACTACGAGCCATATGTGGTGGGGGTCGGTACGATCATTTGCTTTCTACTTTCGGTGGCGATGATATTCCTGCGTGTGGCTTCGGCTTTGGCGATGCTGTTATAGTTGAGGTAAGTCTTAAGCTGATCTCCGCGCTACTAATCAAGATTCTACCTATTCTTTCTCTATATTAGAAACACTAGAGTGCATGAATAGTCAAATTAGACCTAAACCTTAAACTTGAATCAATGTTGTAACCAATACCTTTGAGTTGAGTTACATTAGAAAATTGTCCACGATCCAACTAAAAAACCTCTTAAAGTTGATAAAATAATCAACTTAGCTACAAATTTTCAAAATAAACAAAATAAGGGAGGGAACTGAAAGTTCAAAGTTAAAATTAGTCAAGTAGAAAGGATCACACTTTTTAGTCATAATTCTCTTTGGCTACTCCAGTCTTAGATATTTGTTATGGTCTTGGTGGGTGGGTTTTACCTGTAGTTGCTGAAGGAAAAGAATCTGGTGCCACAACTGAGTCTTGAAGTAGAGAACATAGTGTGTGCTCTGGATCCTGATCTCCAAGGTGCAGCTTCTACAGTTGCTACACTTCTCAGGGAAAAAGGTCAAAGTGTTGATTTGGTTTTGGAGAACAAACCTCTTAAGTGGTATCTCTCTCTTCTCCTTTCCTTCACCAAACATAAATGGTGATCGGATTTTCTGATGCAAAATGGTTGCTTATCTTTTGCAGGGTGTTCAAACGAGCTGCCCGGATTAACGCTCAGCGGCTCATTTTGGTCGGAAGTTCAGAATGGCAACGAGGTATGGTCGGAGTCAAAAATCTTTCGTCGGGCGAACAGTGTGAGGTCAAACTTGATGAACTAAAC

mRNA sequence

ATGGCTGCGAAGCCTGCGTCTCTCCTTATCTTCCGATCCCGCCTCAATCTATACTTCAAGCCCTTCATTTTCTCTTCTTCATCCCTCGCTTTCACTATCACTCTCAGATTTCCCTTTCCGAAGCCCTCATTCTTCGCCAATCGCACGTTTCATTCTTCTCTCAGCGCCGTCTCTTCCGCCCAGTCTCTCGGCGACAGCGGCAGCGCTGGCGGCAGGTCGGGGGCGCTTTCTCCATCGCCGGTCACGGAAGAACTGCAGAAGATCGATGTCAACCCTCCTAAGGGAACTCGAGATTTCCCACCCGAAGATATGCGACTCCGCAACTGGCTATTCCATAATTTCAGGGAGGTTTCGCGAGTCTACGGGTTTGAGGAGATTGATTTTCCGGTGCTCGAGTCGGAGGCGCTGTTCATTAGGAAAGCGGGTGAAGAAATAAGAGACCAGCTCTATTGTTTTGAAGATCGGGGAAATCGTCGAGTTGCTTTACGGCCTGAGCTTACCCCTTCTTTGGCAAGATTAGTGATCCAGAAAGGAAAATCTCTGTCCCTCCCATTGAAATGGTTTGCTATAGGACAATGCTGGCGATATGAGAGAATGACGAGAGGTCGGCGTCGTGAGCATTACCAATGGAATATGGATATCATTGGTGTACCCGAGGTTACGGCTGAAGCAGAGCTTCTATCCTCCATTGTTACTTTTTTCAAGCGAATTGGAATTACAGCAGCTGATGTTGGATTTAAGGTTTCCAGCCGAAAGGTTTTGCAAGAAGTATTAGAATGTTTTGGAATACCTGAAAATATGTTTGGCAAGGTTTGCATTATCATAGACAAGATAGAGAAAATTCCATTAGATGAGATGAAGAAGGAACTGAAAGCTGTGGGTGTATCAGAGGAGGCTATTGAGGAGCTGTTGCAAGTTCTTTCAGTAAAGTCTTTGACAAAGCTAGAAGAGATAATTGGAGGTGGTGGGGAAGCGGTGTCTGATCTGAAGCAGCTGTTTTCTCTCGCTGAAAAGTTTGGGTACGCTGATTGGATCCAATTCGACGCCTCCATTGTCCGCGGCCTTGCTTATTATACTGGGATTGTTTTTGAGGGATTTGATAGAGGAGGAAAACTACGAGCCATATGTGGTGGGGGTCGGTACGATCATTTGCTTTCTACTTTCGGTGGCGATGATATTCCTGCGTGTGGCTTCGGCTTTGGCGATGCTGTTATAGTTGAGTTGCTGAAGGAAAAGAATCTGGTGCCACAACTGAGTCTTGAAGTAGAGAACATAGTGTGTGCTCTGGATCCTGATCTCCAAGGTGCAGCTTCTACAGTTGCTACACTTCTCAGGGAAAAAGGTCAAAGTGTTGATTTGGTTTTGGAGAACAAACCTCTTAAGTGGGTGTTCAAACGAGCTGCCCGGATTAACGCTCAGCGGCTCATTTTGGTCGGAAGTTCAGAATGGCAACGAGGTATGGTCGGAGTCAAAAATCTTTCGTCGGGCGAACAGTGTGAGGTCAAACTTGATGAACTAAAC

Coding sequence (CDS)

ATGGCTGCGAAGCCTGCGTCTCTCCTTATCTTCCGATCCCGCCTCAATCTATACTTCAAGCCCTTCATTTTCTCTTCTTCATCCCTCGCTTTCACTATCACTCTCAGATTTCCCTTTCCGAAGCCCTCATTCTTCGCCAATCGCACGTTTCATTCTTCTCTCAGCGCCGTCTCTTCCGCCCAGTCTCTCGGCGACAGCGGCAGCGCTGGCGGCAGGTCGGGGGCGCTTTCTCCATCGCCGGTCACGGAAGAACTGCAGAAGATCGATGTCAACCCTCCTAAGGGAACTCGAGATTTCCCACCCGAAGATATGCGACTCCGCAACTGGCTATTCCATAATTTCAGGGAGGTTTCGCGAGTCTACGGGTTTGAGGAGATTGATTTTCCGGTGCTCGAGTCGGAGGCGCTGTTCATTAGGAAAGCGGGTGAAGAAATAAGAGACCAGCTCTATTGTTTTGAAGATCGGGGAAATCGTCGAGTTGCTTTACGGCCTGAGCTTACCCCTTCTTTGGCAAGATTAGTGATCCAGAAAGGAAAATCTCTGTCCCTCCCATTGAAATGGTTTGCTATAGGACAATGCTGGCGATATGAGAGAATGACGAGAGGTCGGCGTCGTGAGCATTACCAATGGAATATGGATATCATTGGTGTACCCGAGGTTACGGCTGAAGCAGAGCTTCTATCCTCCATTGTTACTTTTTTCAAGCGAATTGGAATTACAGCAGCTGATGTTGGATTTAAGGTTTCCAGCCGAAAGGTTTTGCAAGAAGTATTAGAATGTTTTGGAATACCTGAAAATATGTTTGGCAAGGTTTGCATTATCATAGACAAGATAGAGAAAATTCCATTAGATGAGATGAAGAAGGAACTGAAAGCTGTGGGTGTATCAGAGGAGGCTATTGAGGAGCTGTTGCAAGTTCTTTCAGTAAAGTCTTTGACAAAGCTAGAAGAGATAATTGGAGGTGGTGGGGAAGCGGTGTCTGATCTGAAGCAGCTGTTTTCTCTCGCTGAAAAGTTTGGGTACGCTGATTGGATCCAATTCGACGCCTCCATTGTCCGCGGCCTTGCTTATTATACTGGGATTGTTTTTGAGGGATTTGATAGAGGAGGAAAACTACGAGCCATATGTGGTGGGGGTCGGTACGATCATTTGCTTTCTACTTTCGGTGGCGATGATATTCCTGCGTGTGGCTTCGGCTTTGGCGATGCTGTTATAGTTGAGTTGCTGAAGGAAAAGAATCTGGTGCCACAACTGAGTCTTGAAGTAGAGAACATAGTGTGTGCTCTGGATCCTGATCTCCAAGGTGCAGCTTCTACAGTTGCTACACTTCTCAGGGAAAAAGGTCAAAGTGTTGATTTGGTTTTGGAGAACAAACCTCTTAAGTGGGTGTTCAAACGAGCTGCCCGGATTAACGCTCAGCGGCTCATTTTGGTCGGAAGTTCAGAATGGCAACGAGGTATGGTCGGAGTCAAAAATCTTTCGTCGGGCGAACAGTGTGAGGTCAAACTTGATGAACTAAAC

Protein sequence

MAAKPASLLIFRSRLNLYFKPFIFSSSSLAFTITLRFPFPKPSFFANRTFHSSLSAVSSAQSLGDSGSAGGRSGALSPSPVTEELQKIDVNPPKGTRDFPPEDMRLRNWLFHNFREVSRVYGFEEIDFPVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKSLSLPLKWFAIGQCWRYERMTRGRRREHYQWNMDIIGVPEVTAEAELLSSIVTFFKRIGITAADVGFKVSSRKVLQEVLECFGIPENMFGKVCIIIDKIEKIPLDEMKKELKAVGVSEEAIEELLQVLSVKSLTKLEEIIGGGGEAVSDLKQLFSLAEKFGYADWIQFDASIVRGLAYYTGIVFEGFDRGGKLRAICGGGRYDHLLSTFGGDDIPACGFGFGDAVIVELLKEKNLVPQLSLEVENIVCALDPDLQGAASTVATLLREKGQSVDLVLENKPLKWVFKRAARINAQRLILVGSSEWQRGMVGVKNLSSGEQCEVKLDELN

Homology

[Close]

BLAST of MS015815 vs. NCBI nr
Match: XP_022143841.1 (histidine--tRNA ligase, chloroplastic/mitochondrial isoform X1 [Momordica charantia])

HSP 1 Score: 995.3 bits (2572), Expect = 1.9e-286
Identity = 506/507 (99.80%), Postives = 507/507 (100.00%), Query Frame = 0


Query: 1   MAAKPASLLIFRSRLNLYFKPFIFSSSSLAFTITLRFPFPKPSFFANRTFHSSLSAVSSA 60
           MAAKPASLLIFRSRLNLYFKPFIFSSSSLAFTITLRFPFPKPSFFANRTFHSSLSAVSSA
Sbjct: 1   MAAKPASLLIFRSRLNLYFKPFIFSSSSLAFTITLRFPFPKPSFFANRTFHSSLSAVSSA 60

Query: 61  QSLGDSGSAGGRSGALSPSPVTEELQKIDVNPPKGTRDFPPEDMRLRNWLFHNFREVSRV 120
           QSLGDSGSAGGRSGALSPSPVTEELQKIDVNPPKGTRDFPPEDMRLRNWLFHNFREVSRV
Sbjct: 61  QSLGDSGSAGGRSGALSPSPVTEELQKIDVNPPKGTRDFPPEDMRLRNWLFHNFREVSRV 120

Query: 121 YGFEEIDFPVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKS 180
           YGFEEIDFPVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKS
Sbjct: 121 YGFEEIDFPVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKS 180

Query: 181 LSLPLKWFAIGQCWRYERMTRGRRREHYQWNMDIIGVPEVTAEAELLSSIVTFFKRIGIT 240
           LSLPLKWFAIGQCWRYERMTRGRRREHYQWNMDIIGVPEVTAEAELLSSIVTFFKRIGIT
Sbjct: 181 LSLPLKWFAIGQCWRYERMTRGRRREHYQWNMDIIGVPEVTAEAELLSSIVTFFKRIGIT 240

Query: 241 AADVGFKVSSRKVLQEVLECFGIPENMFGKVCIIIDKIEKIPLDEMKKELKAVGVSEEAI 300
           AADVGFKVSSRKVLQEVLECFGIPENMFGKVCIIIDKIEKIPLDEMKKELKAVGVSEEAI
Sbjct: 241 AADVGFKVSSRKVLQEVLECFGIPENMFGKVCIIIDKIEKIPLDEMKKELKAVGVSEEAI 300

Query: 301 EELLQVLSVKSLTKLEEIIGGGGEAVSDLKQLFSLAEKFGYADWIQFDASIVRGLAYYTG 360
           EELLQVLSVKSLTKLEEIIGGGGEAVSDLKQLFSLAEKFGYADWIQFDASIVRGLAYYTG
Sbjct: 301 EELLQVLSVKSLTKLEEIIGGGGEAVSDLKQLFSLAEKFGYADWIQFDASIVRGLAYYTG 360

Query: 361 IVFEGFDRGGKLRAICGGGRYDHLLSTFGGDDIPACGFGFGDAVIVELLKEKNLVPQLSL 420
           IVFEGFDRGGKLRAICGGGRYDHLLSTFGGDDIPACGFGFGDAVIVELLKEK+LVPQLSL
Sbjct: 361 IVFEGFDRGGKLRAICGGGRYDHLLSTFGGDDIPACGFGFGDAVIVELLKEKSLVPQLSL 420

Query: 421 EVENIVCALDPDLQGAASTVATLLREKGQSVDLVLENKPLKWVFKRAARINAQRLILVGS 480
           EVENIVCALDPDLQGAASTVATLLREKGQSVDLVLENKPLKWVFKRAARINAQRLILVGS
Sbjct: 421 EVENIVCALDPDLQGAASTVATLLREKGQSVDLVLENKPLKWVFKRAARINAQRLILVGS 480

Query: 481 SEWQRGMVGVKNLSSGEQCEVKLDELN 508
           SEWQRGMVGVKNLSSGEQCEVKLDELN
Sbjct: 481 SEWQRGMVGVKNLSSGEQCEVKLDELN 507

[Close]

BLAST of MS015815 vs. NCBI nr
Match: XP_022143842.1 (histidine--tRNA ligase, chloroplastic/mitochondrial isoform X2 [Momordica charantia])

HSP 1 Score: 983.0 bits (2540), Expect = 9.5e-283
Identity = 500/502 (99.60%), Postives = 501/502 (99.80%), Query Frame = 0


Query: 1   MAAKPASLLIFRSRLNLYFKPFIFSSSSLAFTITLRFPFPKPSFFANRTFHSSLSAVSSA 60
           MAAKPASLLIFRSRLNLYFKPFIFSSSSLAFTITLRFPFPKPSFFANRTFHSSLSAVSSA
Sbjct: 1   MAAKPASLLIFRSRLNLYFKPFIFSSSSLAFTITLRFPFPKPSFFANRTFHSSLSAVSSA 60

Query: 61  QSLGDSGSAGGRSGALSPSPVTEELQKIDVNPPKGTRDFPPEDMRLRNWLFHNFREVSRV 120
           QSLGDSGSAGGRSGALSPSPVTEELQKIDVNPPKGTRDFPPEDMRLRNWLFHNFREVSRV
Sbjct: 61  QSLGDSGSAGGRSGALSPSPVTEELQKIDVNPPKGTRDFPPEDMRLRNWLFHNFREVSRV 120

Query: 121 YGFEEIDFPVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKS 180
           YGFEEIDFPVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKS
Sbjct: 121 YGFEEIDFPVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKS 180

Query: 181 LSLPLKWFAIGQCWRYERMTRGRRREHYQWNMDIIGVPEVTAEAELLSSIVTFFKRIGIT 240
           LSLPLKWFAIGQCWRYERMTRGRRREHYQWNMDIIGVPEVTAEAELLSSIVTFFKRIGIT
Sbjct: 181 LSLPLKWFAIGQCWRYERMTRGRRREHYQWNMDIIGVPEVTAEAELLSSIVTFFKRIGIT 240

Query: 241 AADVGFKVSSRKVLQEVLECFGIPENMFGKVCIIIDKIEKIPLDEMKKELKAVGVSEEAI 300
           AADVGFKVSSRKVLQEVLECFGIPENMFGKVCIIIDKIEKIPLDEMKKELKAVGVSEEAI
Sbjct: 241 AADVGFKVSSRKVLQEVLECFGIPENMFGKVCIIIDKIEKIPLDEMKKELKAVGVSEEAI 300

Query: 301 EELLQVLSVKSLTKLEEIIGGGGEAVSDLKQLFSLAEKFGYADWIQFDASIVRGLAYYTG 360
           EELLQVLSVKSLTKLEEIIGGGGEAVSDLKQLFSLAEKFGYADWIQFDASIVRGLAYYTG
Sbjct: 301 EELLQVLSVKSLTKLEEIIGGGGEAVSDLKQLFSLAEKFGYADWIQFDASIVRGLAYYTG 360

Query: 361 IVFEGFDRGGKLRAICGGGRYDHLLSTFGGDDIPACGFGFGDAVIVELLKEKNLVPQLSL 420
           IVFEGFDRGGKLRAICGGGRYDHLLSTFGGDDIPACGFGFGDAVIVELLKEK+LVPQLSL
Sbjct: 361 IVFEGFDRGGKLRAICGGGRYDHLLSTFGGDDIPACGFGFGDAVIVELLKEKSLVPQLSL 420

Query: 421 EVENIVCALDPDLQGAASTVATLLREKGQSVDLVLENKPLKWVFKRAARINAQRLILVGS 480
           EVENIVCALDPDLQGAASTVATLLREKGQSVDLVLENKPLKWVFKRAARINAQRLILVGS
Sbjct: 421 EVENIVCALDPDLQGAASTVATLLREKGQSVDLVLENKPLKWVFKRAARINAQRLILVGS 480

Query: 481 SEWQRGMVGVKNLSSGEQCEVK 503
           SEWQRGMVGVKNLSSGEQCE K
Sbjct: 481 SEWQRGMVGVKNLSSGEQCEEK 502

[Close]

BLAST of MS015815 vs. NCBI nr
Match: XP_022143843.1 (histidine--tRNA ligase, chloroplastic/mitochondrial isoform X3 [Momordica charantia])

HSP 1 Score: 955.7 bits (2469), Expect = 1.6e-274
Identity = 487/491 (99.19%), Postives = 488/491 (99.39%), Query Frame = 0


Query: 1   MAAKPASLLIFRSRLNLYFKPFIFSSSSLAFTITLRFPFPKPSFFANRTFHSSLSAVSSA 60
           MAAKPASLLIFRSRLNLYFKPFIFSSSSLAFTITLRFPFPKPSFFANRTFHSSLSAVSSA
Sbjct: 1   MAAKPASLLIFRSRLNLYFKPFIFSSSSLAFTITLRFPFPKPSFFANRTFHSSLSAVSSA 60

Query: 61  QSLGDSGSAGGRSGALSPSPVTEELQKIDVNPPKGTRDFPPEDMRLRNWLFHNFREVSRV 120
           QSLGDSGSAGGRSGALSPSPVTEELQKIDVNPPKGTRDFPPEDMRLRNWLFHNFREVSRV
Sbjct: 61  QSLGDSGSAGGRSGALSPSPVTEELQKIDVNPPKGTRDFPPEDMRLRNWLFHNFREVSRV 120

Query: 121 YGFEEIDFPVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKS 180
           YGFEEIDFPVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKS
Sbjct: 121 YGFEEIDFPVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKS 180

Query: 181 LSLPLKWFAIGQCWRYERMTRGRRREHYQWNMDIIGVPEVTAEAELLSSIVTFFKRIGIT 240
           LSLPLKWFAIGQCWRYERMTRGRRREHYQWNMDIIGVPEVTAEAELLSSIVTFFKRIGIT
Sbjct: 181 LSLPLKWFAIGQCWRYERMTRGRRREHYQWNMDIIGVPEVTAEAELLSSIVTFFKRIGIT 240

Query: 241 AADVGFKVSSRKVLQEVLECFGIPENMFGKVCIIIDKIEKIPLDEMKKELKAVGVSEEAI 300
           AADVGFKVSSRKVLQEVLECFGIPENMFGKVCIIIDKIEKIPLDEMKKELKAVGVSEEAI
Sbjct: 241 AADVGFKVSSRKVLQEVLECFGIPENMFGKVCIIIDKIEKIPLDEMKKELKAVGVSEEAI 300

Query: 301 EELLQVLSVKSLTKLEEIIGGGGEAVSDLKQLFSLAEKFGYADWIQFDASIVRGLAYYTG 360
           EELLQVLSVKSLTKLEEIIGGGGEAVSDLKQLFSLAEKFGYADWIQFDASIVRGLAYYTG
Sbjct: 301 EELLQVLSVKSLTKLEEIIGGGGEAVSDLKQLFSLAEKFGYADWIQFDASIVRGLAYYTG 360

Query: 361 IVFEGFDRGGKLRAICGGGRYDHLLSTFGGDDIPACGFGFGDAVIVELLKEKNLVPQLSL 420
           IVFEGFDRGGKLRAICGGGRYDHLLSTFGGDDIPACGFGFGDAVIVELLKEK+LVPQLSL
Sbjct: 361 IVFEGFDRGGKLRAICGGGRYDHLLSTFGGDDIPACGFGFGDAVIVELLKEKSLVPQLSL 420

Query: 421 EVENIVCALDPDLQGAASTVATLLREKGQSVDLVLENKPLKWVFKRAARINAQRLILVGS 480
           EVENIVCALDPDLQGAASTVATLLREKGQSVDLVLENKPLKWVFKRAARINAQRLILVGS
Sbjct: 421 EVENIVCALDPDLQGAASTVATLLREKGQSVDLVLENKPLKWVFKRAARINAQRLILVGS 480

Query: 481 SEWQRGMVGVK 492
           SEWQRG   VK
Sbjct: 481 SEWQRGKKEVK 491

[Close]

BLAST of MS015815 vs. NCBI nr
Match: XP_038883346.1 (histidine--tRNA ligase, chloroplastic/mitochondrial [Benincasa hispida])

HSP 1 Score: 929.5 bits (2401), Expect = 1.3e-266
Identity = 460/507 (90.73%), Postives = 493/507 (97.24%), Query Frame = 0


Query: 1   MAAKPASLLIFRSRLNLYFKPFIFSSSSLAFTITLRFPFPKPSFFANRTFHSSLSAVSSA 60
           MA KP+SLLIF+ RLNL  KPFIFSSSSLAF+I L+FPFPKPSFF NR FHSSL+AVSSA
Sbjct: 1   MATKPSSLLIFQPRLNLRLKPFIFSSSSLAFSIILKFPFPKPSFFVNRNFHSSLTAVSSA 60

Query: 61  QSLGDSGSAGGRSGALSPSPVTEELQKIDVNPPKGTRDFPPEDMRLRNWLFHNFREVSRV 120
           QS+ D G++GGRSGALSP+PVTEELQKIDVNPPKGTRDFPPEDMRLRNWLF+NFREVSRV
Sbjct: 61  QSITDGGTSGGRSGALSPAPVTEELQKIDVNPPKGTRDFPPEDMRLRNWLFNNFREVSRV 120

Query: 121 YGFEEIDFPVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKS 180
           YGFEE+D+PVLESEALF+RKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKS
Sbjct: 121 YGFEEVDYPVLESEALFVRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKS 180

Query: 181 LSLPLKWFAIGQCWRYERMTRGRRREHYQWNMDIIGVPEVTAEAELLSSIVTFFKRIGIT 240
           LSLP+KWFAIGQCWRYERMTRGRRREHYQWNMDIIGVPEVTAEAELLSSIVTFFKR+GIT
Sbjct: 181 LSLPIKWFAIGQCWRYERMTRGRRREHYQWNMDIIGVPEVTAEAELLSSIVTFFKRVGIT 240

Query: 241 AADVGFKVSSRKVLQEVLECFGIPENMFGKVCIIIDKIEKIPLDEMKKELKAVGVSEEAI 300
           A+DVGFKVSSRKVLQ+VL+C+GIPENMFGKVCIIIDKIEKIPLDE+KKELK VG+SE+AI
Sbjct: 241 ASDVGFKVSSRKVLQDVLKCYGIPENMFGKVCIIIDKIEKIPLDEIKKELKVVGISEDAI 300

Query: 301 EELLQVLSVKSLTKLEEIIGGGGEAVSDLKQLFSLAEKFGYADWIQFDASIVRGLAYYTG 360
           EELLQVLSVKSLTKLEE++GGGGEA+SDLKQLFSLAEKFGY+DWIQFDASIVRGLAYYTG
Sbjct: 301 EELLQVLSVKSLTKLEEVLGGGGEALSDLKQLFSLAEKFGYSDWIQFDASIVRGLAYYTG 360

Query: 361 IVFEGFDRGGKLRAICGGGRYDHLLSTFGGDDIPACGFGFGDAVIVELLKEKNLVPQLSL 420
           IVFEGFDRGGKLRAICGGGRYDHLLSTFGGDDIPACGFGFGDAVIVELLKEK L+P+L+ 
Sbjct: 361 IVFEGFDRGGKLRAICGGGRYDHLLSTFGGDDIPACGFGFGDAVIVELLKEKKLLPELNA 420

Query: 421 EVENIVCALDPDLQGAASTVATLLREKGQSVDLVLENKPLKWVFKRAARINAQRLILVGS 480
           EVENI+CALDPDLQGAASTVAT+LREKGQSVDLVLENKPLKWVFKRA RINA+RLILVGS
Sbjct: 421 EVENIICALDPDLQGAASTVATILREKGQSVDLVLENKPLKWVFKRATRINARRLILVGS 480

Query: 481 SEWQRGMVGVKNLSSGEQCEVKLDELN 508
           SEWQRGMVGVK+LSSGEQ EVKLDELN
Sbjct: 481 SEWQRGMVGVKDLSSGEQREVKLDELN 507

[Close]

BLAST of MS015815 vs. NCBI nr
Match: XP_022978407.1 (histidine--tRNA ligase, chloroplastic/mitochondrial isoform X2 [Cucurbita maxima])

HSP 1 Score: 927.2 bits (2395), Expect = 6.2e-266
Identity = 465/507 (91.72%), Postives = 490/507 (96.65%), Query Frame = 0


Query: 1   MAAKPASLLIFRSRLNLYFKPFIFSSSSLAFTITLRFPFPKPSFFANRTFHSSLSAVSSA 60
           MAAKP+SLLIF+ RLNL  KPFIFSS+SLAF+I LR PFPKPSFF NR FHSSLSAVSSA
Sbjct: 1   MAAKPSSLLIFQPRLNLRLKPFIFSSTSLAFSIILRIPFPKPSFFGNRKFHSSLSAVSSA 60

Query: 61  QSLGDSGSAGGRSGALSPSPVTEELQKIDVNPPKGTRDFPPEDMRLRNWLFHNFREVSRV 120
           QS+GD G+ GGRSGALSP+ VTEELQKIDVNPPKGTRDFPPEDMRLRNWLF+NFREVSRV
Sbjct: 61  QSIGDGGNGGGRSGALSPTLVTEELQKIDVNPPKGTRDFPPEDMRLRNWLFNNFREVSRV 120

Query: 121 YGFEEIDFPVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKS 180
           YGFEE+DFPVLESEALFIRKAGEEIR+QLYCFEDRGNRRVALRPELTPSLARLVIQKGKS
Sbjct: 121 YGFEEVDFPVLESEALFIRKAGEEIREQLYCFEDRGNRRVALRPELTPSLARLVIQKGKS 180

Query: 181 LSLPLKWFAIGQCWRYERMTRGRRREHYQWNMDIIGVPEVTAEAELLSSIVTFFKRIGIT 240
           LSLPLKWFAIGQCWRYERMTRGRRREHYQWNMDIIGVPEVTAEAELLSSIV FFKRIG+T
Sbjct: 181 LSLPLKWFAIGQCWRYERMTRGRRREHYQWNMDIIGVPEVTAEAELLSSIVAFFKRIGLT 240

Query: 241 AADVGFKVSSRKVLQEVLECFGIPENMFGKVCIIIDKIEKIPLDEMKKELKAVGVSEEAI 300
           AADVGFKVSSRKVLQEVL+C+GIPENMFGKVCIIIDKIEKIPLDEMKKELK VGVSE+AI
Sbjct: 241 AADVGFKVSSRKVLQEVLKCYGIPENMFGKVCIIIDKIEKIPLDEMKKELKVVGVSEDAI 300

Query: 301 EELLQVLSVKSLTKLEEIIGGGGEAVSDLKQLFSLAEKFGYADWIQFDASIVRGLAYYTG 360
           EE+LQVLSVKSLTKLEEI+GGGGEA+SDLKQLFSLAEKFGY+DWIQFDASIVRGLAYYTG
Sbjct: 301 EEMLQVLSVKSLTKLEEILGGGGEAMSDLKQLFSLAEKFGYSDWIQFDASIVRGLAYYTG 360

Query: 361 IVFEGFDRGGKLRAICGGGRYDHLLSTFGGDDIPACGFGFGDAVIVELLKEKNLVPQLSL 420
           IVFEGFDRGGKLRAICGGGRYD LLSTFGGDD+PACGFGFGDAVIVELLKEK L+P+LS+
Sbjct: 361 IVFEGFDRGGKLRAICGGGRYDRLLSTFGGDDLPACGFGFGDAVIVELLKEKKLLPELSV 420

Query: 421 EVENIVCALDPDLQGAASTVATLLREKGQSVDLVLENKPLKWVFKRAARINAQRLILVGS 480
           EVENIVCALDPDLQGAAS VAT+LREKGQ+VDLVLENKPLKWVFKRA RINAQRLILVGS
Sbjct: 421 EVENIVCALDPDLQGAASRVATILREKGQTVDLVLENKPLKWVFKRATRINAQRLILVGS 480

Query: 481 SEWQRGMVGVKNLSSGEQCEVKLDELN 508
           SEWQRGMVGVK+LSSGEQ EVKLDELN
Sbjct: 481 SEWQRGMVGVKDLSSGEQREVKLDELN 507

[Close]

BLAST of MS015815 vs. ExPASy Swiss-Prot
Match: O82413 (Histidine--tRNA ligase, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At3g46100 PE=2 SV=1)

HSP 1 Score: 742.3 bits (1915), Expect = 3.7e-213
Identity = 363/443 (81.94%), Postives = 413/443 (93.23%), Query Frame = 0


Query: 66  SGSAGGRSGALSPSPVTEE--LQKIDVNPPKGTRDFPPEDMRLRNWLFHNFREVSRVYGF 125
           + + GGRSG++    VTEE   +KIDVNPPKGTRDFPPEDMRLRNWLF++F+EVSR+YG+
Sbjct: 43  AANGGGRSGSIVAPLVTEEDFHKKIDVNPPKGTRDFPPEDMRLRNWLFNHFKEVSRLYGY 102

Query: 126 EEIDFPVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKSLSL 185
           EE+D+PVLE+EALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKS+SL
Sbjct: 103 EEVDYPVLETEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKSVSL 162

Query: 186 PLKWFAIGQCWRYERMTRGRRREHYQWNMDIIGVPEVTAEAELLSSIVTFFKRIGITAAD 245
           PLKWFAIGQCWRYERMTRGRRREHYQWNMDIIGVP+VTAEAEL+SSIVTFFKRIGITA+D
Sbjct: 163 PLKWFAIGQCWRYERMTRGRRREHYQWNMDIIGVPQVTAEAELISSIVTFFKRIGITASD 222

Query: 246 VGFKVSSRKVLQEVLECFGIPENMFGKVCIIIDKIEKIPLDEMKKELKAVGVSEEAIEEL 305
           VGFKVSSRKVLQE+L+ +G+PE++FG+VCIIIDKIEKIP+DE+KKEL   G+SE+AIE+L
Sbjct: 223 VGFKVSSRKVLQELLKKYGVPEDLFGRVCIIIDKIEKIPIDEIKKELGFTGISEDAIEQL 282

Query: 306 LQVLSVKSLTKLEEIIGGGGEAVSDLKQLFSLAEKFGYADWIQFDASIVRGLAYYTGIVF 365
           LQVLSVKSL  LE+IIGG GEA++DLKQLFSLAEKFGY++WIQFDAS+VRGLAYYTGIVF
Sbjct: 283 LQVLSVKSLDDLEDIIGGAGEAIADLKQLFSLAEKFGYSEWIQFDASVVRGLAYYTGIVF 342

Query: 366 EGFDRGGKLRAICGGGRYDHLLSTFGGDDIPACGFGFGDAVIVELLKEKNLVPQLSLEVE 425
           EGFDR GKLRAICGGGRYD LLST+GGDD PACGFGFGDAVIVELLKEK+L+P+L  EVE
Sbjct: 343 EGFDRKGKLRAICGGGRYDRLLSTYGGDDFPACGFGFGDAVIVELLKEKDLLPELGQEVE 402

Query: 426 NIVCALDPDLQGAASTVATLLREKGQSVDLVLENKPLKWVFKRAARINAQRLILVGSSEW 485
           NIVCALD DLQGAA+TVAT LR+KGQ+VDLVLE+KPLKWVFKRAAR+NA+RL+LVG +EW
Sbjct: 403 NIVCALDKDLQGAAATVATALRDKGQTVDLVLESKPLKWVFKRAARVNARRLVLVGKTEW 462

Query: 486 QRGMVGVKNLSSGEQCEVKLDEL 507
           + G V VK LSSGEQ +VKL +L
Sbjct: 463 EDGSVSVKVLSSGEQFQVKLSDL 485

[Close]

BLAST of MS015815 vs. ExPASy Swiss-Prot
Match: Q9HLX5 (Histidine--tRNA ligase OS=Thermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165) OX=273075 GN=hisS PE=1 SV=1)

HSP 1 Score: 275.0 bits (702), Expect = 1.7e-72
Identity = 151/424 (35.61%), Postives = 244/424 (57.55%), Query Frame = 0


Query: 85  LQKIDVNPPKGTRDFPPEDMRLRNWLFHNFREVSRVYGFEEIDFPVLESEALFIRKAGEE 144
           + ++ +   +G RDF PEDM +  ++F    E +  +GF  IDFP LE   L+  K+GEE
Sbjct: 1   MYRLQIEKIRGFRDFYPEDMDVEKFIFKTAEEAAEAFGFRRIDFPSLEYLDLYRIKSGEE 60

Query: 145 IRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKSLSLPLKWFAIGQCWRYERMTRGRR 204
           +  Q Y F D+G R V L PE TPS  R+V  + K L  PL+W++  + WRYE    GR 
Sbjct: 61  LLQQTYSFVDKGGREVTLIPEATPSTVRMVTSR-KDLQRPLRWYSFPKVWRYEEPQAGRY 120

Query: 205 REHYQWNMDIIGVPEVTAEAELLSSIVTFFKRIGITAADVGFKVSSRKVLQEVLECFGIP 264
           REHYQ+N DI G     A+AE+++   +   R+G+       +++SRK+++E++   G+ 
Sbjct: 121 REHYQFNADIFGSDSPEADAEVIALASSILDRLGLQDI-YEIRINSRKIMEEIIG--GMT 180

Query: 265 ENMFGKVCIIIDKIEKIPLDEMKKELKAVGVSEEAIEELLQVLS-VKSLTKLEEIIGGGG 324
            +    V  IID+  KI  +E   +L++ G+ E+ +  +  + S  + + ++  I G   
Sbjct: 181 SSDPFSVFSIIDRYHKISREEFVDQLRSAGIGEDGVSMIADLCSGTRGIDEMARITGKSS 240

Query: 325 EAVSDLKQLFSLAEKFGYADWIQFDASIVRGLAYYTGIVFEGFDRGGKLRAICGGGRYDH 384
           E ++ +  +  L   +G  + +++D SIVRGL+YYTGIVFE +DR G+ RAI GGGRYD+
Sbjct: 241 EEIARMAAVEDLLASYGVKN-VRYDFSIVRGLSYYTGIVFEAYDRSGQFRAILGGGRYDN 300

Query: 385 LLSTFGGDDIPACGFGFGDAVIVELLKEKNLVPQLSLEVENI-VCALDPDLQGAASTVAT 444
           L S   G+ +PA GFG GDAVI  LLK +N+  Q+  E +++ +C +        +  + 
Sbjct: 301 LASLMSGESVPAVGFGMGDAVISLLLKRENV--QIPREKKSVYICRVGKINSSIMNEYSR 360

Query: 445 LLREKGQSVDLVLENKPLKWVFKRAARINAQRLILVGSSEWQRGMVGVKNLSSGEQCEVK 504
            LRE+G +V + +  + L    K A+ I A   ++ G  + +RG+V ++N+ +G Q  V 
Sbjct: 361 KLRERGMNVTVEIMERGLSAQLKYASAIGADFAVIFGERDLERGVVTIRNMYTGSQENVG 417

Query: 505 LDEL 507
           LD +
Sbjct: 421 LDSV 417

[Close]

BLAST of MS015815 vs. ExPASy Swiss-Prot
Match: B1ZUW6 (Histidine--tRNA ligase OS=Opitutus terrae (strain DSM 11246 / JCM 15787 / PB90-1) OX=452637 GN=hisS PE=3 SV=1)

HSP 1 Score: 265.8 bits (678), Expect = 1.0e-69
Identity = 151/419 (36.04%), Postives = 236/419 (56.32%), Query Frame = 0


Query: 95  GTRDFPPEDMRLRNWLFHNFREVSRVYGFEEIDFPVLESEALFIRKAGEEIRDQLYCFED 154
           G R+F P+ +  RN +F  +R+ +  +GF E D PVLE   L+  K+G+EI  QL+ F D
Sbjct: 9   GFREFYPDALARRNHIFRLWRQTAVAFGFAEYDAPVLEPLELYKTKSGDEIEAQLFSFTD 68

Query: 155 RGNRRVALRPELTPSLARLVIQKGKSLSLPLKWFAIGQCWRYERMTRGRRREHYQWNMDI 214
           +G R VALRPE+TP++ R+V  K  +L  P+KWF+I + +RYER  +GR R  +Q+N D+
Sbjct: 69  KGGREVALRPEMTPTVCRMVGAKANALKRPIKWFSIAEYYRYERAQKGRERAFFQFNADL 128

Query: 215 IGVPEVTAEAELLSSIVTFFKRIGITAADVGFKVSSRKVLQEVLECFGIPENMFGKVCII 274
              P   AE EL++ +    K  G+T  D   ++S R +    LE  G+ E     V   
Sbjct: 129 FAEPGPEAEIELIALLTQCLKAFGLTEQDFYIRLSDRNLWFFYLEALGLDEPRIRAVLSA 188

Query: 275 IDKIEKIPLDEMKKELKAVGVSEEAIEE-LLQVLSVKSLTKLEEIIGG-GGEAVS----D 334
           +DK EK+  D  K   +  G  + A+++ +L+ L +K+L  LE+ +   GGE ++    D
Sbjct: 189 VDKFEKVGDDAFKPYAEQFGPLDLALKQRVLEFLQIKTLASLEQTLAPLGGEKLAARLGD 248

Query: 335 LKQLFSLAEKFGYADWIQFDASIVRGLAYYTGIVFEGFDRGGKLRAICGGGRYDHLLSTF 394
            ++L       G + +I+ D  +VRGLAYYTG VFE FDR G+LRAI GGGRY+ L+   
Sbjct: 249 WRKLLDGLAAMGLSPFIEVDLGVVRGLAYYTGFVFEAFDRKGELRAIAGGGRYNDLVKKL 308

Query: 395 GGDDIPACGFGFGDAVIVELLKEKNLVPQLSLEVENIVCALDPDLQ-GAASTVATLLREK 454
           GG D+PA GF  GD  +  LL  + L+P   ++  ++ C +    +  AA      LR  
Sbjct: 309 GGPDLPAVGFAIGDVTLGLLLDARGLMPAF-VQASDVYCVIGGAAERQAAFADVNALRAA 368

Query: 455 GQSVDLVLENKPLKWVFKRAARINAQRLILVGSSEWQRGMVGVKNLSSGEQCEVKLDEL 507
           G  VD  L++ P    FK AA   A+  ++ G  E  + +V +++L++  + +V  +++
Sbjct: 369 GFKVDYPLKDVPFGKQFKLAADSGAKLALIYGPDELAKNVVKLRDLTTRTETDVPREQV 426

[Close]

BLAST of MS015815 vs. ExPASy Swiss-Prot
Match: Q8U431 (Histidine--tRNA ligase OS=Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) OX=186497 GN=hisS PE=3 SV=1)

HSP 1 Score: 264.2 bits (674), Expect = 3.0e-69
Identity = 148/423 (34.99%), Postives = 240/423 (56.74%), Query Frame = 0


Query: 94  KGTRDFPPEDMRLRNWLFHNFREVSRVYGFEEIDFPVLESEALFIRKAGEEIRDQLYCFE 153
           KGTRDF PE+M  R W+F   RE    YGF+E+  P +E   LF  ++GEE+  QLY F+
Sbjct: 6   KGTRDFLPEEMVKRRWVFERIRERFETYGFKEVLTPTMEYTKLFELRSGEEVVKQLYAFK 65

Query: 154 DRGNRRVALRPELTPSLARLVIQKGKSLSLPLKWFAIGQCWRYERMTRGRRREHYQWNMD 213
           D+G R V+LRP++T S+ARL +   ++   P+KW+ I   +RYE    GR RE +Q  ++
Sbjct: 66  DKGGRDVSLRPDMTSSVARLYVNMFQTAPKPIKWYYIANMFRYEEPQSGRYREFWQAGVE 125

Query: 214 IIGVPEVTAEAELLSSIVTFFKRIGITAADVGFKVSSRKVLQEVLECFGIPENMFGKVCI 273
           +IG  +V A+AE+++  V  +   G+    V   +  R +L E  +  G+ +++   +  
Sbjct: 126 LIGSDKVEADAEVIALFVESYLATGLREFTV--NIGDRILLDEFAKMLGVKDDI--GLMR 185

Query: 274 IIDKIEKIPLDEMKKELKAVGVSEEAIEELLQVLSVKS-----LTKLEEIIGG--GGEAV 333
           IIDK +K+P ++    LK  G+ +  IE++L+++++K      L   EE+         +
Sbjct: 186 IIDKKDKLPQEDFINALKEFGLDKNGIEKVLELINIKGKPDEVLPLAEELFTSEVAKNEI 245

Query: 334 SDLKQLFSLAEKFGYADWIQFDASIVRGLAYYTGIVFEGF---DRGGKLRAICGGGRYDH 393
           + L  L  L E +   DWI+ D  I RG  YYT IVFE     D G  + +I GGGRYD+
Sbjct: 246 NRLYALIDLLEAYEVKDWIRIDLGIARGFDYYTSIVFEAIAPNDLG--IGSIGGGGRYDN 305

Query: 394 LLSTFGGDDIPACGFGFGDAVIVELLKEKNLVPQLSLEVENIVCALDPDLQGAASTVATL 453
           L+  FGG   PA GF  G   ++ +L+ K L+P++ +  +  V  +   ++  A  +AT 
Sbjct: 306 LIEVFGGKPTPATGFAIGIERLIPILEAKGLIPEVQIAPDVYVVPIGERMKKVAIRIATS 365

Query: 454 LREKGQSVDLVLENKPLKWVFKRAARINAQRLILVGSSEWQRGMVGVKNLSSGEQCEVKL 507
           LR+ G   ++ L  + LK     A ++  ++ I+VG  + +RG+V V+++ SGEQ EVK+
Sbjct: 366 LRKSGIKTEIELMGRKLKKAMDYANKVGVKKTIIVGEKDLERGVVTVRDMESGEQNEVKI 422

[Close]

BLAST of MS015815 vs. ExPASy Swiss-Prot
Match: O58028 (Histidine--tRNA ligase OS=Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) OX=70601 GN=hisS PE=3 SV=1)

HSP 1 Score: 261.9 bits (668), Expect = 1.5e-68
Identity = 155/421 (36.82%), Postives = 234/421 (55.58%), Query Frame = 0


Query: 94  KGTRDFPPEDMRLRNWLFHNFREVSRVYGFEEIDFPVLESEALFIRKAGEEIRDQLYCFE 153
           KGTRDF PEDM  R W+F   REV   YGF+EI  PV+E   LF  ++GEE+  QLY F+
Sbjct: 6   KGTRDFLPEDMAKRRWVFEKIREVFEAYGFKEILTPVMEYTKLFQLRSGEEVVKQLYAFK 65

Query: 154 DRGNRRVALRPELTPSLARLVIQKGKSLSLPLKWFAIGQCWRYERMTRGRRREHYQWNMD 213
           D+G R V+LRP++T S+ARL +   +    P+KW+ I   +RYE    GR RE +Q  ++
Sbjct: 66  DKGGRDVSLRPDMTSSVARLYVNSFQMAPKPIKWYYIANMFRYEEPQSGRYREFWQAGVE 125

Query: 214 IIGVPEVTAEAELLSSIVTFFKRIGITAADVGFKVSSRKVLQEVLECFGIPENMFGKVCI 273
           +IG  +V A+AE+++  V  +   G+   D    +  R +L E     G+ +++   +  
Sbjct: 126 LIGSDKVEADAEVIALFVESYLSTGL--RDFTVNIGDRILLDEFAGMLGVSDDI--GLMR 185

Query: 274 IIDKIEKIPLDEMKKELKAVGVSEEAIEELLQVLSVKS-----LTKLEEIIGG--GGEAV 333
           IIDK +K+  +E    LK  G+ E  IE++L+++ +K      L   EE+       E +
Sbjct: 186 IIDKRDKLSQEEFINLLKEFGLGESEIEKVLELVEIKGRPDNVLPLAEELFKSKKAKEEI 245

Query: 334 SDLKQLFSLAEKFGYADWIQFDASIVRGLAYYTGIVFEGFDRGG-KLRAICGGGRYDHLL 393
           S L +L  L E +G  DWI+ D  I RG  YYT IVFE        + +I GGGRYD+L+
Sbjct: 246 SKLYKLTDLLEWYGVKDWIRIDLGIARGFDYYTSIVFEAISPNELGIGSIGGGGRYDNLI 305

Query: 394 STFGGDDIPACGFGFGDAVIVELLKEKNLVPQLSLEVENIVCALDPDLQGAASTVATLLR 453
             FGG   PA GF  G   ++ +L+ K L+P+     E  V  L  D++  A  +A  LR
Sbjct: 306 EIFGGKPTPATGFAIGIERLLPILEWKGLIPKPQTGPEVFVIPL-KDMEKVAINIAVKLR 365

Query: 454 EKGQSVDLVLENKPLKWVFKRAARINAQRLILVGSSEWQRGMVGVKNLSSGEQCEVKLDE 507
            +    D+ L  + L      A R+ A+ +I+VG  + +RG+V ++++ SGEQ  V L+E
Sbjct: 366 REKIKTDIELSGRKLGKALDYANRVGAKLVIIVGKRDVERGVVTIRDMESGEQYNVSLNE 421

[Close]

BLAST of MS015815 vs. ExPASy TrEMBL
Match: A0A6J1CRI8 (Histidine--tRNA ligase OS=Momordica charantia OX=3673 GN=LOC111013661 PE=3 SV=1)

HSP 1 Score: 995.3 bits (2572), Expect = 9.0e-287
Identity = 506/507 (99.80%), Postives = 507/507 (100.00%), Query Frame = 0


Query: 1   MAAKPASLLIFRSRLNLYFKPFIFSSSSLAFTITLRFPFPKPSFFANRTFHSSLSAVSSA 60
           MAAKPASLLIFRSRLNLYFKPFIFSSSSLAFTITLRFPFPKPSFFANRTFHSSLSAVSSA
Sbjct: 1   MAAKPASLLIFRSRLNLYFKPFIFSSSSLAFTITLRFPFPKPSFFANRTFHSSLSAVSSA 60

Query: 61  QSLGDSGSAGGRSGALSPSPVTEELQKIDVNPPKGTRDFPPEDMRLRNWLFHNFREVSRV 120
           QSLGDSGSAGGRSGALSPSPVTEELQKIDVNPPKGTRDFPPEDMRLRNWLFHNFREVSRV
Sbjct: 61  QSLGDSGSAGGRSGALSPSPVTEELQKIDVNPPKGTRDFPPEDMRLRNWLFHNFREVSRV 120

Query: 121 YGFEEIDFPVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKS 180
           YGFEEIDFPVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKS
Sbjct: 121 YGFEEIDFPVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKS 180

Query: 181 LSLPLKWFAIGQCWRYERMTRGRRREHYQWNMDIIGVPEVTAEAELLSSIVTFFKRIGIT 240
           LSLPLKWFAIGQCWRYERMTRGRRREHYQWNMDIIGVPEVTAEAELLSSIVTFFKRIGIT
Sbjct: 181 LSLPLKWFAIGQCWRYERMTRGRRREHYQWNMDIIGVPEVTAEAELLSSIVTFFKRIGIT 240

Query: 241 AADVGFKVSSRKVLQEVLECFGIPENMFGKVCIIIDKIEKIPLDEMKKELKAVGVSEEAI 300
           AADVGFKVSSRKVLQEVLECFGIPENMFGKVCIIIDKIEKIPLDEMKKELKAVGVSEEAI
Sbjct: 241 AADVGFKVSSRKVLQEVLECFGIPENMFGKVCIIIDKIEKIPLDEMKKELKAVGVSEEAI 300

Query: 301 EELLQVLSVKSLTKLEEIIGGGGEAVSDLKQLFSLAEKFGYADWIQFDASIVRGLAYYTG 360
           EELLQVLSVKSLTKLEEIIGGGGEAVSDLKQLFSLAEKFGYADWIQFDASIVRGLAYYTG
Sbjct: 301 EELLQVLSVKSLTKLEEIIGGGGEAVSDLKQLFSLAEKFGYADWIQFDASIVRGLAYYTG 360

Query: 361 IVFEGFDRGGKLRAICGGGRYDHLLSTFGGDDIPACGFGFGDAVIVELLKEKNLVPQLSL 420
           IVFEGFDRGGKLRAICGGGRYDHLLSTFGGDDIPACGFGFGDAVIVELLKEK+LVPQLSL
Sbjct: 361 IVFEGFDRGGKLRAICGGGRYDHLLSTFGGDDIPACGFGFGDAVIVELLKEKSLVPQLSL 420

Query: 421 EVENIVCALDPDLQGAASTVATLLREKGQSVDLVLENKPLKWVFKRAARINAQRLILVGS 480
           EVENIVCALDPDLQGAASTVATLLREKGQSVDLVLENKPLKWVFKRAARINAQRLILVGS
Sbjct: 421 EVENIVCALDPDLQGAASTVATLLREKGQSVDLVLENKPLKWVFKRAARINAQRLILVGS 480

Query: 481 SEWQRGMVGVKNLSSGEQCEVKLDELN 508
           SEWQRGMVGVKNLSSGEQCEVKLDELN
Sbjct: 481 SEWQRGMVGVKNLSSGEQCEVKLDELN 507

[Close]

BLAST of MS015815 vs. ExPASy TrEMBL
Match: A0A6J1CS13 (Histidine--tRNA ligase OS=Momordica charantia OX=3673 GN=LOC111013661 PE=3 SV=1)

HSP 1 Score: 983.0 bits (2540), Expect = 4.6e-283
Identity = 500/502 (99.60%), Postives = 501/502 (99.80%), Query Frame = 0


Query: 1   MAAKPASLLIFRSRLNLYFKPFIFSSSSLAFTITLRFPFPKPSFFANRTFHSSLSAVSSA 60
           MAAKPASLLIFRSRLNLYFKPFIFSSSSLAFTITLRFPFPKPSFFANRTFHSSLSAVSSA
Sbjct: 1   MAAKPASLLIFRSRLNLYFKPFIFSSSSLAFTITLRFPFPKPSFFANRTFHSSLSAVSSA 60

Query: 61  QSLGDSGSAGGRSGALSPSPVTEELQKIDVNPPKGTRDFPPEDMRLRNWLFHNFREVSRV 120
           QSLGDSGSAGGRSGALSPSPVTEELQKIDVNPPKGTRDFPPEDMRLRNWLFHNFREVSRV
Sbjct: 61  QSLGDSGSAGGRSGALSPSPVTEELQKIDVNPPKGTRDFPPEDMRLRNWLFHNFREVSRV 120

Query: 121 YGFEEIDFPVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKS 180
           YGFEEIDFPVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKS
Sbjct: 121 YGFEEIDFPVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKS 180

Query: 181 LSLPLKWFAIGQCWRYERMTRGRRREHYQWNMDIIGVPEVTAEAELLSSIVTFFKRIGIT 240
           LSLPLKWFAIGQCWRYERMTRGRRREHYQWNMDIIGVPEVTAEAELLSSIVTFFKRIGIT
Sbjct: 181 LSLPLKWFAIGQCWRYERMTRGRRREHYQWNMDIIGVPEVTAEAELLSSIVTFFKRIGIT 240

Query: 241 AADVGFKVSSRKVLQEVLECFGIPENMFGKVCIIIDKIEKIPLDEMKKELKAVGVSEEAI 300
           AADVGFKVSSRKVLQEVLECFGIPENMFGKVCIIIDKIEKIPLDEMKKELKAVGVSEEAI
Sbjct: 241 AADVGFKVSSRKVLQEVLECFGIPENMFGKVCIIIDKIEKIPLDEMKKELKAVGVSEEAI 300

Query: 301 EELLQVLSVKSLTKLEEIIGGGGEAVSDLKQLFSLAEKFGYADWIQFDASIVRGLAYYTG 360
           EELLQVLSVKSLTKLEEIIGGGGEAVSDLKQLFSLAEKFGYADWIQFDASIVRGLAYYTG
Sbjct: 301 EELLQVLSVKSLTKLEEIIGGGGEAVSDLKQLFSLAEKFGYADWIQFDASIVRGLAYYTG 360

Query: 361 IVFEGFDRGGKLRAICGGGRYDHLLSTFGGDDIPACGFGFGDAVIVELLKEKNLVPQLSL 420
           IVFEGFDRGGKLRAICGGGRYDHLLSTFGGDDIPACGFGFGDAVIVELLKEK+LVPQLSL
Sbjct: 361 IVFEGFDRGGKLRAICGGGRYDHLLSTFGGDDIPACGFGFGDAVIVELLKEKSLVPQLSL 420

Query: 421 EVENIVCALDPDLQGAASTVATLLREKGQSVDLVLENKPLKWVFKRAARINAQRLILVGS 480
           EVENIVCALDPDLQGAASTVATLLREKGQSVDLVLENKPLKWVFKRAARINAQRLILVGS
Sbjct: 421 EVENIVCALDPDLQGAASTVATLLREKGQSVDLVLENKPLKWVFKRAARINAQRLILVGS 480

Query: 481 SEWQRGMVGVKNLSSGEQCEVK 503
           SEWQRGMVGVKNLSSGEQCE K
Sbjct: 481 SEWQRGMVGVKNLSSGEQCEEK 502

[Close]

BLAST of MS015815 vs. ExPASy TrEMBL
Match: A0A6J1CRQ7 (Histidine--tRNA ligase OS=Momordica charantia OX=3673 GN=LOC111013661 PE=3 SV=1)

HSP 1 Score: 955.7 bits (2469), Expect = 7.9e-275
Identity = 487/491 (99.19%), Postives = 488/491 (99.39%), Query Frame = 0


Query: 1   MAAKPASLLIFRSRLNLYFKPFIFSSSSLAFTITLRFPFPKPSFFANRTFHSSLSAVSSA 60
           MAAKPASLLIFRSRLNLYFKPFIFSSSSLAFTITLRFPFPKPSFFANRTFHSSLSAVSSA
Sbjct: 1   MAAKPASLLIFRSRLNLYFKPFIFSSSSLAFTITLRFPFPKPSFFANRTFHSSLSAVSSA 60

Query: 61  QSLGDSGSAGGRSGALSPSPVTEELQKIDVNPPKGTRDFPPEDMRLRNWLFHNFREVSRV 120
           QSLGDSGSAGGRSGALSPSPVTEELQKIDVNPPKGTRDFPPEDMRLRNWLFHNFREVSRV
Sbjct: 61  QSLGDSGSAGGRSGALSPSPVTEELQKIDVNPPKGTRDFPPEDMRLRNWLFHNFREVSRV 120

Query: 121 YGFEEIDFPVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKS 180
           YGFEEIDFPVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKS
Sbjct: 121 YGFEEIDFPVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKS 180

Query: 181 LSLPLKWFAIGQCWRYERMTRGRRREHYQWNMDIIGVPEVTAEAELLSSIVTFFKRIGIT 240
           LSLPLKWFAIGQCWRYERMTRGRRREHYQWNMDIIGVPEVTAEAELLSSIVTFFKRIGIT
Sbjct: 181 LSLPLKWFAIGQCWRYERMTRGRRREHYQWNMDIIGVPEVTAEAELLSSIVTFFKRIGIT 240

Query: 241 AADVGFKVSSRKVLQEVLECFGIPENMFGKVCIIIDKIEKIPLDEMKKELKAVGVSEEAI 300
           AADVGFKVSSRKVLQEVLECFGIPENMFGKVCIIIDKIEKIPLDEMKKELKAVGVSEEAI
Sbjct: 241 AADVGFKVSSRKVLQEVLECFGIPENMFGKVCIIIDKIEKIPLDEMKKELKAVGVSEEAI 300

Query: 301 EELLQVLSVKSLTKLEEIIGGGGEAVSDLKQLFSLAEKFGYADWIQFDASIVRGLAYYTG 360
           EELLQVLSVKSLTKLEEIIGGGGEAVSDLKQLFSLAEKFGYADWIQFDASIVRGLAYYTG
Sbjct: 301 EELLQVLSVKSLTKLEEIIGGGGEAVSDLKQLFSLAEKFGYADWIQFDASIVRGLAYYTG 360

Query: 361 IVFEGFDRGGKLRAICGGGRYDHLLSTFGGDDIPACGFGFGDAVIVELLKEKNLVPQLSL 420
           IVFEGFDRGGKLRAICGGGRYDHLLSTFGGDDIPACGFGFGDAVIVELLKEK+LVPQLSL
Sbjct: 361 IVFEGFDRGGKLRAICGGGRYDHLLSTFGGDDIPACGFGFGDAVIVELLKEKSLVPQLSL 420

Query: 421 EVENIVCALDPDLQGAASTVATLLREKGQSVDLVLENKPLKWVFKRAARINAQRLILVGS 480
           EVENIVCALDPDLQGAASTVATLLREKGQSVDLVLENKPLKWVFKRAARINAQRLILVGS
Sbjct: 421 EVENIVCALDPDLQGAASTVATLLREKGQSVDLVLENKPLKWVFKRAARINAQRLILVGS 480

Query: 481 SEWQRGMVGVK 492
           SEWQRG   VK
Sbjct: 481 SEWQRGKKEVK 491

[Close]

BLAST of MS015815 vs. ExPASy TrEMBL
Match: A0A6J1IL10 (Histidine--tRNA ligase OS=Cucurbita maxima OX=3661 GN=LOC111478405 PE=3 SV=1)

HSP 1 Score: 927.2 bits (2395), Expect = 3.0e-266
Identity = 465/507 (91.72%), Postives = 490/507 (96.65%), Query Frame = 0


Query: 1   MAAKPASLLIFRSRLNLYFKPFIFSSSSLAFTITLRFPFPKPSFFANRTFHSSLSAVSSA 60
           MAAKP+SLLIF+ RLNL  KPFIFSS+SLAF+I LR PFPKPSFF NR FHSSLSAVSSA
Sbjct: 1   MAAKPSSLLIFQPRLNLRLKPFIFSSTSLAFSIILRIPFPKPSFFGNRKFHSSLSAVSSA 60

Query: 61  QSLGDSGSAGGRSGALSPSPVTEELQKIDVNPPKGTRDFPPEDMRLRNWLFHNFREVSRV 120
           QS+GD G+ GGRSGALSP+ VTEELQKIDVNPPKGTRDFPPEDMRLRNWLF+NFREVSRV
Sbjct: 61  QSIGDGGNGGGRSGALSPTLVTEELQKIDVNPPKGTRDFPPEDMRLRNWLFNNFREVSRV 120

Query: 121 YGFEEIDFPVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKS 180
           YGFEE+DFPVLESEALFIRKAGEEIR+QLYCFEDRGNRRVALRPELTPSLARLVIQKGKS
Sbjct: 121 YGFEEVDFPVLESEALFIRKAGEEIREQLYCFEDRGNRRVALRPELTPSLARLVIQKGKS 180

Query: 181 LSLPLKWFAIGQCWRYERMTRGRRREHYQWNMDIIGVPEVTAEAELLSSIVTFFKRIGIT 240
           LSLPLKWFAIGQCWRYERMTRGRRREHYQWNMDIIGVPEVTAEAELLSSIV FFKRIG+T
Sbjct: 181 LSLPLKWFAIGQCWRYERMTRGRRREHYQWNMDIIGVPEVTAEAELLSSIVAFFKRIGLT 240

Query: 241 AADVGFKVSSRKVLQEVLECFGIPENMFGKVCIIIDKIEKIPLDEMKKELKAVGVSEEAI 300
           AADVGFKVSSRKVLQEVL+C+GIPENMFGKVCIIIDKIEKIPLDEMKKELK VGVSE+AI
Sbjct: 241 AADVGFKVSSRKVLQEVLKCYGIPENMFGKVCIIIDKIEKIPLDEMKKELKVVGVSEDAI 300

Query: 301 EELLQVLSVKSLTKLEEIIGGGGEAVSDLKQLFSLAEKFGYADWIQFDASIVRGLAYYTG 360
           EE+LQVLSVKSLTKLEEI+GGGGEA+SDLKQLFSLAEKFGY+DWIQFDASIVRGLAYYTG
Sbjct: 301 EEMLQVLSVKSLTKLEEILGGGGEAMSDLKQLFSLAEKFGYSDWIQFDASIVRGLAYYTG 360

Query: 361 IVFEGFDRGGKLRAICGGGRYDHLLSTFGGDDIPACGFGFGDAVIVELLKEKNLVPQLSL 420
           IVFEGFDRGGKLRAICGGGRYD LLSTFGGDD+PACGFGFGDAVIVELLKEK L+P+LS+
Sbjct: 361 IVFEGFDRGGKLRAICGGGRYDRLLSTFGGDDLPACGFGFGDAVIVELLKEKKLLPELSV 420

Query: 421 EVENIVCALDPDLQGAASTVATLLREKGQSVDLVLENKPLKWVFKRAARINAQRLILVGS 480
           EVENIVCALDPDLQGAAS VAT+LREKGQ+VDLVLENKPLKWVFKRA RINAQRLILVGS
Sbjct: 421 EVENIVCALDPDLQGAASRVATILREKGQTVDLVLENKPLKWVFKRATRINAQRLILVGS 480

Query: 481 SEWQRGMVGVKNLSSGEQCEVKLDELN 508
           SEWQRGMVGVK+LSSGEQ EVKLDELN
Sbjct: 481 SEWQRGMVGVKDLSSGEQREVKLDELN 507

[Close]

BLAST of MS015815 vs. ExPASy TrEMBL
Match: A0A6J1EG18 (Histidine--tRNA ligase OS=Cucurbita moschata OX=3662 GN=LOC111433048 PE=3 SV=1)

HSP 1 Score: 921.8 bits (2381), Expect = 1.3e-264
Identity = 464/507 (91.52%), Postives = 487/507 (96.06%), Query Frame = 0


Query: 1   MAAKPASLLIFRSRLNLYFKPFIFSSSSLAFTITLRFPFPKPSFFANRTFHSSLSAVSSA 60
           MAAKP+SLLIF+ RLNL  KPFIFSSSSLAF+I LR PFPK SFF NR FHSSLSAVSSA
Sbjct: 1   MAAKPSSLLIFQPRLNLRLKPFIFSSSSLAFSIILRIPFPKASFFGNRKFHSSLSAVSSA 60

Query: 61  QSLGDSGSAGGRSGALSPSPVTEELQKIDVNPPKGTRDFPPEDMRLRNWLFHNFREVSRV 120
           QS GD G+ GGRSGALSP+ VTEELQKIDVNPPKGTRDFPPEDMRLRNWLF+NFREVSRV
Sbjct: 61  QSTGDGGNGGGRSGALSPTLVTEELQKIDVNPPKGTRDFPPEDMRLRNWLFNNFREVSRV 120

Query: 121 YGFEEIDFPVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKS 180
           YGFEE+DFPVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKS
Sbjct: 121 YGFEEVDFPVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKS 180

Query: 181 LSLPLKWFAIGQCWRYERMTRGRRREHYQWNMDIIGVPEVTAEAELLSSIVTFFKRIGIT 240
           LSLPLKWFAIGQCWRYERMTRGRRREHYQWNMDIIGVPEVTAEAELLSSIV FFKRIG+T
Sbjct: 181 LSLPLKWFAIGQCWRYERMTRGRRREHYQWNMDIIGVPEVTAEAELLSSIVAFFKRIGLT 240

Query: 241 AADVGFKVSSRKVLQEVLECFGIPENMFGKVCIIIDKIEKIPLDEMKKELKAVGVSEEAI 300
           AADVGFKVSSRKVLQEVL+C+GIPENMFGKVCIIIDKIEKIPLDEMKKELK VGVSE+AI
Sbjct: 241 AADVGFKVSSRKVLQEVLKCYGIPENMFGKVCIIIDKIEKIPLDEMKKELKVVGVSEDAI 300

Query: 301 EELLQVLSVKSLTKLEEIIGGGGEAVSDLKQLFSLAEKFGYADWIQFDASIVRGLAYYTG 360
           EE+LQVLSVKSLTKLEEI+GGGGEA+SDLKQLFSLAEKFGY+DWIQFDASIVRGLAYYTG
Sbjct: 301 EEMLQVLSVKSLTKLEEILGGGGEAMSDLKQLFSLAEKFGYSDWIQFDASIVRGLAYYTG 360

Query: 361 IVFEGFDRGGKLRAICGGGRYDHLLSTFGGDDIPACGFGFGDAVIVELLKEKNLVPQLSL 420
           IVFEGFDRGGKLRAICGGGRYD LLSTFGGDD+PACGFGFGDAVIVELLKEK L+P+L++
Sbjct: 361 IVFEGFDRGGKLRAICGGGRYDRLLSTFGGDDLPACGFGFGDAVIVELLKEKKLLPELNV 420

Query: 421 EVENIVCALDPDLQGAASTVATLLREKGQSVDLVLENKPLKWVFKRAARINAQRLILVGS 480
           EVENIVCALDPDLQGAAS VAT+LR KGQ+VDLVLENKPLKWVFKRA RINAQRLILVGS
Sbjct: 421 EVENIVCALDPDLQGAASRVATILRGKGQTVDLVLENKPLKWVFKRATRINAQRLILVGS 480

Query: 481 SEWQRGMVGVKNLSSGEQCEVKLDELN 508
           SEWQRGMVGVK+LSSGEQ EVKLDELN
Sbjct: 481 SEWQRGMVGVKDLSSGEQREVKLDELN 507

[Close]

BLAST of MS015815 vs. TAIR 10
Match: AT3G46100.1 (Histidyl-tRNA synthetase 1 )

HSP 1 Score: 742.3 bits (1915), Expect = 2.6e-214
Identity = 363/443 (81.94%), Postives = 413/443 (93.23%), Query Frame = 0


Query: 66  SGSAGGRSGALSPSPVTEE--LQKIDVNPPKGTRDFPPEDMRLRNWLFHNFREVSRVYGF 125
           + + GGRSG++    VTEE   +KIDVNPPKGTRDFPPEDMRLRNWLF++F+EVSR+YG+
Sbjct: 43  AANGGGRSGSIVAPLVTEEDFHKKIDVNPPKGTRDFPPEDMRLRNWLFNHFKEVSRLYGY 102

Query: 126 EEIDFPVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKSLSL 185
           EE+D+PVLE+EALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKS+SL
Sbjct: 103 EEVDYPVLETEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKSVSL 162

Query: 186 PLKWFAIGQCWRYERMTRGRRREHYQWNMDIIGVPEVTAEAELLSSIVTFFKRIGITAAD 245
           PLKWFAIGQCWRYERMTRGRRREHYQWNMDIIGVP+VTAEAEL+SSIVTFFKRIGITA+D
Sbjct: 163 PLKWFAIGQCWRYERMTRGRRREHYQWNMDIIGVPQVTAEAELISSIVTFFKRIGITASD 222

Query: 246 VGFKVSSRKVLQEVLECFGIPENMFGKVCIIIDKIEKIPLDEMKKELKAVGVSEEAIEEL 305
           VGFKVSSRKVLQE+L+ +G+PE++FG+VCIIIDKIEKIP+DE+KKEL   G+SE+AIE+L
Sbjct: 223 VGFKVSSRKVLQELLKKYGVPEDLFGRVCIIIDKIEKIPIDEIKKELGFTGISEDAIEQL 282

Query: 306 LQVLSVKSLTKLEEIIGGGGEAVSDLKQLFSLAEKFGYADWIQFDASIVRGLAYYTGIVF 365
           LQVLSVKSL  LE+IIGG GEA++DLKQLFSLAEKFGY++WIQFDAS+VRGLAYYTGIVF
Sbjct: 283 LQVLSVKSLDDLEDIIGGAGEAIADLKQLFSLAEKFGYSEWIQFDASVVRGLAYYTGIVF 342

Query: 366 EGFDRGGKLRAICGGGRYDHLLSTFGGDDIPACGFGFGDAVIVELLKEKNLVPQLSLEVE 425
           EGFDR GKLRAICGGGRYD LLST+GGDD PACGFGFGDAVIVELLKEK+L+P+L  EVE
Sbjct: 343 EGFDRKGKLRAICGGGRYDRLLSTYGGDDFPACGFGFGDAVIVELLKEKDLLPELGQEVE 402

Query: 426 NIVCALDPDLQGAASTVATLLREKGQSVDLVLENKPLKWVFKRAARINAQRLILVGSSEW 485
           NIVCALD DLQGAA+TVAT LR+KGQ+VDLVLE+KPLKWVFKRAAR+NA+RL+LVG +EW
Sbjct: 403 NIVCALDKDLQGAAATVATALRDKGQTVDLVLESKPLKWVFKRAARVNARRLVLVGKTEW 462

Query: 486 QRGMVGVKNLSSGEQCEVKLDEL 507
           + G V VK LSSGEQ +VKL +L
Sbjct: 463 EDGSVSVKVLSSGEQFQVKLSDL 485

[Close]

BLAST of MS015815 vs. TAIR 10
Match: AT5G03406.1 (Class II aaRS and biotin synthetases superfamily protein )

HSP 1 Score: 378.3 bits (970), Expect = 1.0e-104
Identity = 178/218 (81.65%), Postives = 205/218 (94.04%), Query Frame = 0


Query: 68  SAGGRSGAL-SPSPVTEELQKIDVNPPKGTRDFPPEDMRLRNWLFHNFREVSRVYGFEEI 127
           + GGRSG++ +P  V EE Q IDVNPPKGTRDFPPEDMRLRNWLF++F+EVSR++G+EE+
Sbjct: 46  NGGGRSGSIVAPLVVNEEFQNIDVNPPKGTRDFPPEDMRLRNWLFNHFKEVSRLFGYEEV 105

Query: 128 DFPVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKSLSLPLK 187
           DFPVLE+EALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKS+SLPLK
Sbjct: 106 DFPVLETEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKSVSLPLK 165

Query: 188 WFAIGQCWRYERMTRGRRREHYQWNMDIIGVPEVTAEAELLSSIVTFFKRIGITAADVGF 247
           WFA+GQCWRYERMTRGRRREHYQWNMDIIGVPEVTAEAEL+SSI+TFFKRIGITA+DVGF
Sbjct: 166 WFAVGQCWRYERMTRGRRREHYQWNMDIIGVPEVTAEAELISSIMTFFKRIGITASDVGF 225

Query: 248 KVSSRKVLQEVLECFGIPENMFGKVCIIIDKIEKIPLD 285
           KVSSRKVLQE L+ +G+PE++FG+VC IIDK+  + ++
Sbjct: 226 KVSSRKVLQEFLKKYGVPEDLFGRVCFIIDKVSNLNIN 263

[Close]

BLAST of MS015815 vs. TAIR 10
Match: AT3G02760.1 (Class II aaRS and biotin synthetases superfamily protein )

HSP 1 Score: 167.2 bits (422), Expect = 3.5e-41
Identity = 131/423 (30.97%), Postives = 206/423 (48.70%), Query Frame = 0


Query: 93  PKGTRDFPPEDMRLRNWLFHNFREVSRVYGFEEIDFPVLESEALFIRKAGEEIRDQLYCF 152
           PKGTRDF  E M +R   F   + V + +G   +D PV E     + K GE+ +  +Y  
Sbjct: 449 PKGTRDFAKEQMAVREKAFSIIQNVFKRHGATALDTPVFELRETLMGKYGEDSK-LVYDI 508

Query: 153 EDRGNRRVALRPELTPSLARLVIQKGKSLSLPLKWFAIGQCWRYERMTRGRRREHYQWNM 212
            D+G    +LR +LT   AR V   G +     K + I + +R +  ++GR RE YQ + 
Sbjct: 509 ADQGGELCSLRYDLTVPFARYVAMNGIT---SFKRYQIAKVYRRDNPSKGRYREFYQCDF 568

Query: 213 DIIGVPE-VTAEAELLSSIVTFFKRIGITAADVGFKVSSRKVLQEVLECFGIPENMFGKV 272
           DI G+ E +  + E++  +      + I   D   K++ RK+L  +LE  G+P   F  +
Sbjct: 569 DIAGLFEPMGPDFEIVKILTELLDELEI--GDYEVKLNHRKLLDGMLEICGVPPEKFRTI 628

Query: 273 CIIIDKIEKIPLDEMKKEL-KAVGVSEEAIEELLQVLSVKS-----LTKL----EEIIG- 332
           C  IDK++K   +++KKE+ +  G+S E  + +   +  K      L+KL     E +  
Sbjct: 629 CSSIDKLDKQSFEQVKKEMVEEKGLSSEIADRIGNFVKEKGAPLELLSKLRQEGSEFLDN 688

Query: 333 -GGGEAVSDLKQLFSLAEKFGYADWIQFDASIVRGLAYYTGIVFEGFDRGGKLRAICGGG 392
               EA+ +L  +F   ++   ++ I FD S+ RGL YYTG++FE    G ++ +I  GG
Sbjct: 689 QSSREALDELSIMFEALKRSKCSERIVFDLSLARGLDYYTGVIFEAVCIGAEVGSIGAGG 748

Query: 393 RYDHLLSTFGGDDIPACGFGFG-DAV--IVELLKEKNLVPQLSLEVENIVCAL-DPDLQG 452
           RYD+L+  FG   +PA G   G + V  I+E L EK        E + +V  + D  L  
Sbjct: 749 RYDNLIGMFGTKQVPAVGMSLGIERVFNIMEELNEKQKQVIRPTETQVLVSIMVDNKLAE 808

Query: 453 AASTVATLLREKGQSVDLVLENKPLKWVFKRAARINAQRLILVGSSEWQRGMVGVKNLSS 499
           AA  V+ L   K  +  LV + K     F RA       +++VG  E     V +K L  
Sbjct: 809 AAELVSQLWGAKINAEYLVSKRKEKH--FNRAKESGIPWMVMVGEKELSGSFVTLKKLEK 863

The following BLAST results are available for this feature:

BLAST of MS015815 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022143841.1	1.9e-286	99.80	histidine--tRNA ligase, chloroplastic/mitochondrial isoform X1 [Momordica charan...	[more]
XP_022143842.1	9.5e-283	99.60	histidine--tRNA ligase, chloroplastic/mitochondrial isoform X2 [Momordica charan...	[more]
XP_022143843.1	1.6e-274	99.19	histidine--tRNA ligase, chloroplastic/mitochondrial isoform X3 [Momordica charan...	[more]
XP_038883346.1	1.3e-266	90.73	histidine--tRNA ligase, chloroplastic/mitochondrial [Benincasa hispida]	[more]
XP_022978407.1	6.2e-266	91.72	histidine--tRNA ligase, chloroplastic/mitochondrial isoform X2 [Cucurbita maxima...	[more]

BLAST of MS015815 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O82413	3.7e-213	81.94	Histidine--tRNA ligase, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3...	[more]
Q9HLX5	1.7e-72	35.61	Histidine--tRNA ligase OS=Thermoplasma acidophilum (strain ATCC 25905 / DSM 1728...	[more]
B1ZUW6	1.0e-69	36.04	Histidine--tRNA ligase OS=Opitutus terrae (strain DSM 11246 / JCM 15787 / PB90-1...	[more]
Q8U431	3.0e-69	34.99	Histidine--tRNA ligase OS=Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JC...	[more]
O58028	1.5e-68	36.82	Histidine--tRNA ligase OS=Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 ...	[more]

BLAST of MS015815 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CRI8	9.0e-287	99.80	Histidine--tRNA ligase OS=Momordica charantia OX=3673 GN=LOC111013661 PE=3 SV=1	[more]
A0A6J1CS13	4.6e-283	99.60	Histidine--tRNA ligase OS=Momordica charantia OX=3673 GN=LOC111013661 PE=3 SV=1	[more]
A0A6J1CRQ7	7.9e-275	99.19	Histidine--tRNA ligase OS=Momordica charantia OX=3673 GN=LOC111013661 PE=3 SV=1	[more]
A0A6J1IL10	3.0e-266	91.72	Histidine--tRNA ligase OS=Cucurbita maxima OX=3661 GN=LOC111478405 PE=3 SV=1	[more]
A0A6J1EG18	1.3e-264	91.52	Histidine--tRNA ligase OS=Cucurbita moschata OX=3662 GN=LOC111433048 PE=3 SV=1	[more]

BLAST of MS015815 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G46100.1	2.6e-214	81.94	Histidyl-tRNA synthetase 1	[more]
AT5G03406.1	1.0e-104	81.65	Class II aaRS and biotin synthetases superfamily protein	[more]
AT3G02760.1	3.5e-41	30.97	Class II aaRS and biotin synthetases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036621	Anticodon-binding domain superfamily	GENE3D	3.40.50.800		coord: 415..507 e-value: 9.2E-18 score: 65.7
IPR041715	Class II Histidinyl-tRNA synthetase (HisRS)-like catalytic core domain	PFAM	PF13393	tRNA-synt_His	coord: 95..399 e-value: 4.5E-47 score: 160.8
IPR041715	Class II Histidinyl-tRNA synthetase (HisRS)-like catalytic core domain	CDD	cd00773	HisRS-like_core	coord: 103..410 e-value: 7.53912E-99 score: 296.821
None	No IPR available	GENE3D	3.30.930.10	Bira Bifunctional Protein; Domain 2	coord: 86..414 e-value: 7.7E-116 score: 388.6
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 65..97
None	No IPR available	PANTHER	PTHR43707:SF1	HISTIDINE--TRNA LIGASE, MITOCHONDRIAL-RELATED	coord: 86..506
None	No IPR available	SUPERFAMILY	52954	Class II aaRS ABD-related	coord: 422..506
None	No IPR available	SUPERFAMILY	55681	Class II aaRS and biotin synthetases	coord: 92..414
IPR004154	Anticodon-binding	PFAM	PF03129	HGTP_anticodon	coord: 428..506 e-value: 1.4E-8 score: 34.7
IPR004516	Histidine-tRNA ligase/ATP phosphoribosyltransferase regulatory subunit	PIRSF	PIRSF001549	His-tRNA_synth	coord: 86..507 e-value: 1.7E-146 score: 486.3
IPR004516	Histidine-tRNA ligase/ATP phosphoribosyltransferase regulatory subunit	PANTHER	PTHR43707	HISTIDYL-TRNA SYNTHETASE	coord: 86..506
IPR015807	Histidine-tRNA ligase	TIGRFAM	TIGR00442	TIGR00442	coord: 92..502 e-value: 1.3E-104 score: 348.3
IPR015807	Histidine-tRNA ligase	HAMAP	MF_00127	His_tRNA_synth	coord: 88..507 score: 25.070925
IPR006195	Aminoacyl-tRNA synthetase, class II	PROSITE	PS50862	AA_TRNA_LIGASE_II	coord: 95..416 score: 13.735352

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS015815.1	MS015815.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006427	histidyl-tRNA aminoacylation
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004821	histidine-tRNA ligase activity