MS015728 (gene) Bitter gourd (TR) v1

Overview
NameMS015728
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
Descriptionwall-associated receptor kinase 2-like
Locationscaffold983: 645734 .. 648178 (+)
RNA-Seq ExpressionMS015728
SyntenyMS015728
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTCGTCGTCCAATGGAGACGCTTATGCGACTAATCATCACGGTGGTTCAAATAGGCATACTTTCAGCGGCGGCGGCGACGGCGGCGGCGGGAGCTTCTCAAGCCTTACCGGGGTGCGAGGAGTGGTGCGGCGATGTGCAGATTCCATATCCGTTCGGCATGAAAGAAGGGTGTTATCTGAATGAAAAATTCTTCATTTCGTGTAACAAAACTCACAACGATTCTCCAAAGGCGTTTCTACAAGGCGGCAACCTCAACGTGACAAATATTTCCATCTCCGGCGAGCTGAACATCTTGCATTTCTGGGCCCGAGATTGCTACCCACCAAACACCGTTCCCGATTTATCCACCGCGTTTCTCAACACGGAGTTCGCCGTTTCCAGCGCCAAAAACAAGTTCACCGTCATCGGCTGCGACACCTTCTCTTTCATCTCCGGCCTCGTCGGCGGGACCCAATTCTACAAGAGCGCCTGCGTGGCGCTCTGCGATGACATCACCACCGTTAAAGACGGTGCCTGCTCCGGCAATGGCTGCTGCCAGCTGGAGATCCCCAAAGGCCTCAACAATTTGAACTACACAGTGAGCAGCTTCGACAATCACACTAATGTGCTGAGTTTCAATCCCTGTGGGTACGCATTTGTAATTGAGGAAGATAAGTTCAATTTCTCCTCCAAATATATTCGCCATTTTCCGCTGGAGAGAGTTCCGTTGGTGCTTGATTGGGCCATCAGTAACAACACTTGCGAGAATACAACCAATTGCATCTGTGGACCCAGCAGCCAAAAGGTTAACTTCGTCGAGGATGGATCGGAATATCGTTGCCAGTGCTCTGATGGCTTTGAGGGGAATCCATATCTCCCTCGAGGTTGTCAAGGTAAATAAAATTAATCACATGCGATAACATCGTATCATTATGGAGATACTTGGGTTTGTTGTTAAAGTTGGGATTCTTTTTGTTTTTTTTTTTTTTTCAGATGTTGATGAATGCAAGGATGGAAGGCATGACTGTAAGTTTGAGTGTGTTAATACAGAAGGAAACTATACGTGCAATTGTCCTGAGGGTTTTAGAGGAGATGGAAGACGCGAGGGAGAAGGTTGCACCAGAAACTCAAAGTCTTTCGTTCAAATCATCGTTGGTGAGCTAATTTAATTTGGTGGTTCTATTTGATTTCTAAGTGGGTTTTTGTTTTTAGGGTTTCTAAATGTTTATTGGTGAAATGAAATAATTATAATGCAGGAGTTACTGTGGGGTTCACAGTTTTGTTGATTGGGCTTACATGGTTCTACTTGGGCTATAGAAAGTGGAAGTTCGTCAAATTGAAAGAGGAATTTTTTGAGAAAAATGGAGGCTTGATGCTCCAACAGCATCTTTCTCAATGGCAAACGTCGGCCGACATGGTCAGAATCTTCACCCAAGAGGAACTGGAGAAGGCCACGAACAAGTACGATGAGAGCGCAGTGGTCGGAAAAGGCGGATACGGCACCGTTTACAAAGGAGTTTTGGAGGATGGTTTGGTAGTTGCAATAAAGAAATCGAAATTAGTGGACCAATCCCAAACTTCCCAGTTCATCAACGAAGTGATGGTTCTGTCCCAAATCAACCACCGCAACGTGGTCAAGCTCTTGGGCTGCTGTTTGGAGACGCAAGTTCCATTATTGGTGTATGAGTTCATCACGAACGGCACACTTTACGACCACATCCATGACAAAGCGAATCACGATTATTCCCTTCCATGGGAAGCCCGCTTGAGAATTGCTTCGGAAACTGCGGGGGTAATTTCGTATTTGCATTCCTCAGCCTCCACTCCAATCATCCACAGAGACATCAAGACCACCAACATACTATTAGACCAAAACTACACTGCCAAAGTCTCTGATTTCGGCGCTTCCAAGTTGGTTCCGTTGGATCAAACTCAGCTGTCTACGATGGTGCAAGGGACTCTCGGGTATCTGGACCCGGAGTACTTGTTGACGAGCGAGTTGACGGAGAAGAGCGACGTGTACAGCTTTGGAATAGTGCTTATGGAGCTCATAACGGGGAAGAAGGCGGTGAGTTTCGAGGGGCCGGAAGCGGAGAGGAATCTGGCGATGTATGTGCTGTGTGCAATGAAGGAGGATCGTTTGGAAGAAGTTGTGGAGAAGGGAATGGCGAGGGAGGGGAAGTTGGAGCAGATAAAAGAAGTGGCAAAGGTGGCAAAAGAGTGTTTGAGAGTAAGGGGGGAGGAGAGACCGAGCATGAAGGAGGTAGCCATGGAGTTGGAGGGATTGAGAGTTTTGGTTGAGAATAAATGGGTTAATGACAATAATTTAATCAATGCAGAAGAGATGGTGGCTTATTTGTTGGAGGATAATAATGGAGCTTCAGAATCTACATTATTGAGCATGAATAATGTTGTGGACGATAGCATGAAGGTTCAGATTTTGCCACGGATCCATGATGGAAGA

mRNA sequence

CTTCGTCGTCCAATGGAGACGCTTATGCGACTAATCATCACGGTGGTTCAAATAGGCATACTTTCAGCGGCGGCGGCGACGGCGGCGGCGGGAGCTTCTCAAGCCTTACCGGGGTGCGAGGAGTGGTGCGGCGATGTGCAGATTCCATATCCGTTCGGCATGAAAGAAGGGTGTTATCTGAATGAAAAATTCTTCATTTCGTGTAACAAAACTCACAACGATTCTCCAAAGGCGTTTCTACAAGGCGGCAACCTCAACGTGACAAATATTTCCATCTCCGGCGAGCTGAACATCTTGCATTTCTGGGCCCGAGATTGCTACCCACCAAACACCGTTCCCGATTTATCCACCGCGTTTCTCAACACGGAGTTCGCCGTTTCCAGCGCCAAAAACAAGTTCACCGTCATCGGCTGCGACACCTTCTCTTTCATCTCCGGCCTCGTCGGCGGGACCCAATTCTACAAGAGCGCCTGCGTGGCGCTCTGCGATGACATCACCACCGTTAAAGACGGTGCCTGCTCCGGCAATGGCTGCTGCCAGCTGGAGATCCCCAAAGGCCTCAACAATTTGAACTACACAGTGAGCAGCTTCGACAATCACACTAATGTGCTGAGTTTCAATCCCTGTGGGTACGCATTTGTAATTGAGGAAGATAAGTTCAATTTCTCCTCCAAATATATTCGCCATTTTCCGCTGGAGAGAGTTCCGTTGGTGCTTGATTGGGCCATCAGTAACAACACTTGCGAGAATACAACCAATTGCATCTGTGGACCCAGCAGCCAAAAGGTTAACTTCGTCGAGGATGGATCGGAATATCGTTGCCAGTGCTCTGATGGCTTTGAGGGGAATCCATATCTCCCTCGAGGTTGTCAAGATGTTGATGAATGCAAGGATGGAAGGCATGACTGTAAGTTTGAGTGTGTTAATACAGAAGGAAACTATACGTGCAATTGTCCTGAGGGTTTTAGAGGAGATGGAAGACGCGAGGGAGAAGGTTGCACCAGAAACTCAAAGTCTTTCGTTCAAATCATCGTTGGAGTTACTGTGGGGTTCACAGTTTTGTTGATTGGGCTTACATGGTTCTACTTGGGCTATAGAAAGTGGAAGTTCGTCAAATTGAAAGAGGAATTTTTTGAGAAAAATGGAGGCTTGATGCTCCAACAGCATCTTTCTCAATGGCAAACGTCGGCCGACATGGTCAGAATCTTCACCCAAGAGGAACTGGAGAAGGCCACGAACAAGTACGATGAGAGCGCAGTGGTCGGAAAAGGCGGATACGGCACCGTTTACAAAGGAGTTTTGGAGGATGGTTTGGTAGTTGCAATAAAGAAATCGAAATTAGTGGACCAATCCCAAACTTCCCAGTTCATCAACGAAGTGATGGTTCTGTCCCAAATCAACCACCGCAACGTGGTCAAGCTCTTGGGCTGCTGTTTGGAGACGCAAGTTCCATTATTGGTGTATGAGTTCATCACGAACGGCACACTTTACGACCACATCCATGACAAAGCGAATCACGATTATTCCCTTCCATGGGAAGCCCGCTTGAGAATTGCTTCGGAAACTGCGGGGGTAATTTCGTATTTGCATTCCTCAGCCTCCACTCCAATCATCCACAGAGACATCAAGACCACCAACATACTATTAGACCAAAACTACACTGCCAAAGTCTCTGATTTCGGCGCTTCCAAGTTGGTTCCGTTGGATCAAACTCAGCTGTCTACGATGGTGCAAGGGACTCTCGGGTATCTGGACCCGGAGTACTTGTTGACGAGCGAGTTGACGGAGAAGAGCGACGTGTACAGCTTTGGAATAGTGCTTATGGAGCTCATAACGGGGAAGAAGGCGGTGAGTTTCGAGGGGCCGGAAGCGGAGAGGAATCTGGCGATGTATGTGCTGTGTGCAATGAAGGAGGATCGTTTGGAAGAAGTTGTGGAGAAGGGAATGGCGAGGGAGGGGAAGTTGGAGCAGATAAAAGAAGTGGCAAAGGTGGCAAAAGAGTGTTTGAGAGTAAGGGGGGAGGAGAGACCGAGCATGAAGGAGGTAGCCATGGAGTTGGAGGGATTGAGAGTTTTGGTTGAGAATAAATGGGTTAATGACAATAATTTAATCAATGCAGAAGAGATGGTGGCTTATTTGTTGGAGGATAATAATGGAGCTTCAGAATCTACATTATTGAGCATGAATAATGTTGTGGACGATAGCATGAAGGTTCAGATTTTGCCACGGATCCATGATGGAAGA

Coding sequence (CDS)

CTTCGTCGTCCAATGGAGACGCTTATGCGACTAATCATCACGGTGGTTCAAATAGGCATACTTTCAGCGGCGGCGGCGACGGCGGCGGCGGGAGCTTCTCAAGCCTTACCGGGGTGCGAGGAGTGGTGCGGCGATGTGCAGATTCCATATCCGTTCGGCATGAAAGAAGGGTGTTATCTGAATGAAAAATTCTTCATTTCGTGTAACAAAACTCACAACGATTCTCCAAAGGCGTTTCTACAAGGCGGCAACCTCAACGTGACAAATATTTCCATCTCCGGCGAGCTGAACATCTTGCATTTCTGGGCCCGAGATTGCTACCCACCAAACACCGTTCCCGATTTATCCACCGCGTTTCTCAACACGGAGTTCGCCGTTTCCAGCGCCAAAAACAAGTTCACCGTCATCGGCTGCGACACCTTCTCTTTCATCTCCGGCCTCGTCGGCGGGACCCAATTCTACAAGAGCGCCTGCGTGGCGCTCTGCGATGACATCACCACCGTTAAAGACGGTGCCTGCTCCGGCAATGGCTGCTGCCAGCTGGAGATCCCCAAAGGCCTCAACAATTTGAACTACACAGTGAGCAGCTTCGACAATCACACTAATGTGCTGAGTTTCAATCCCTGTGGGTACGCATTTGTAATTGAGGAAGATAAGTTCAATTTCTCCTCCAAATATATTCGCCATTTTCCGCTGGAGAGAGTTCCGTTGGTGCTTGATTGGGCCATCAGTAACAACACTTGCGAGAATACAACCAATTGCATCTGTGGACCCAGCAGCCAAAAGGTTAACTTCGTCGAGGATGGATCGGAATATCGTTGCCAGTGCTCTGATGGCTTTGAGGGGAATCCATATCTCCCTCGAGGTTGTCAAGATGTTGATGAATGCAAGGATGGAAGGCATGACTGTAAGTTTGAGTGTGTTAATACAGAAGGAAACTATACGTGCAATTGTCCTGAGGGTTTTAGAGGAGATGGAAGACGCGAGGGAGAAGGTTGCACCAGAAACTCAAAGTCTTTCGTTCAAATCATCGTTGGAGTTACTGTGGGGTTCACAGTTTTGTTGATTGGGCTTACATGGTTCTACTTGGGCTATAGAAAGTGGAAGTTCGTCAAATTGAAAGAGGAATTTTTTGAGAAAAATGGAGGCTTGATGCTCCAACAGCATCTTTCTCAATGGCAAACGTCGGCCGACATGGTCAGAATCTTCACCCAAGAGGAACTGGAGAAGGCCACGAACAAGTACGATGAGAGCGCAGTGGTCGGAAAAGGCGGATACGGCACCGTTTACAAAGGAGTTTTGGAGGATGGTTTGGTAGTTGCAATAAAGAAATCGAAATTAGTGGACCAATCCCAAACTTCCCAGTTCATCAACGAAGTGATGGTTCTGTCCCAAATCAACCACCGCAACGTGGTCAAGCTCTTGGGCTGCTGTTTGGAGACGCAAGTTCCATTATTGGTGTATGAGTTCATCACGAACGGCACACTTTACGACCACATCCATGACAAAGCGAATCACGATTATTCCCTTCCATGGGAAGCCCGCTTGAGAATTGCTTCGGAAACTGCGGGGGTAATTTCGTATTTGCATTCCTCAGCCTCCACTCCAATCATCCACAGAGACATCAAGACCACCAACATACTATTAGACCAAAACTACACTGCCAAAGTCTCTGATTTCGGCGCTTCCAAGTTGGTTCCGTTGGATCAAACTCAGCTGTCTACGATGGTGCAAGGGACTCTCGGGTATCTGGACCCGGAGTACTTGTTGACGAGCGAGTTGACGGAGAAGAGCGACGTGTACAGCTTTGGAATAGTGCTTATGGAGCTCATAACGGGGAAGAAGGCGGTGAGTTTCGAGGGGCCGGAAGCGGAGAGGAATCTGGCGATGTATGTGCTGTGTGCAATGAAGGAGGATCGTTTGGAAGAAGTTGTGGAGAAGGGAATGGCGAGGGAGGGGAAGTTGGAGCAGATAAAAGAAGTGGCAAAGGTGGCAAAAGAGTGTTTGAGAGTAAGGGGGGAGGAGAGACCGAGCATGAAGGAGGTAGCCATGGAGTTGGAGGGATTGAGAGTTTTGGTTGAGAATAAATGGGTTAATGACAATAATTTAATCAATGCAGAAGAGATGGTGGCTTATTTGTTGGAGGATAATAATGGAGCTTCAGAATCTACATTATTGAGCATGAATAATGTTGTGGACGATAGCATGAAGGTTCAGATTTTGCCACGGATCCATGATGGAAGA

Protein sequence

LRRPMETLMRLIITVVQIGILSAAAATAAAGASQALPGCEEWCGDVQIPYPFGMKEGCYLNEKFFISCNKTHNDSPKAFLQGGNLNVTNISISGELNILHFWARDCYPPNTVPDLSTAFLNTEFAVSSAKNKFTVIGCDTFSFISGLVGGTQFYKSACVALCDDITTVKDGACSGNGCCQLEIPKGLNNLNYTVSSFDNHTNVLSFNPCGYAFVIEEDKFNFSSKYIRHFPLERVPLVLDWAISNNTCENTTNCICGPSSQKVNFVEDGSEYRCQCSDGFEGNPYLPRGCQDVDECKDGRHDCKFECVNTEGNYTCNCPEGFRGDGRREGEGCTRNSKSFVQIIVGVTVGFTVLLIGLTWFYLGYRKWKFVKLKEEFFEKNGGLMLQQHLSQWQTSADMVRIFTQEELEKATNKYDESAVVGKGGYGTVYKGVLEDGLVVAIKKSKLVDQSQTSQFINEVMVLSQINHRNVVKLLGCCLETQVPLLVYEFITNGTLYDHIHDKANHDYSLPWEARLRIASETAGVISYLHSSASTPIIHRDIKTTNILLDQNYTAKVSDFGASKLVPLDQTQLSTMVQGTLGYLDPEYLLTSELTEKSDVYSFGIVLMELITGKKAVSFEGPEAERNLAMYVLCAMKEDRLEEVVEKGMAREGKLEQIKEVAKVAKECLRVRGEERPSMKEVAMELEGLRVLVENKWVNDNNLINAEEMVAYLLEDNNGASESTLLSMNNVVDDSMKVQILPRIHDGR
Homology
BLAST of MS015728 vs. NCBI nr
Match: XP_022141581.1 (putative wall-associated receptor kinase-like 16 [Momordica charantia])

HSP 1 Score: 1502.3 bits (3888), Expect = 0.0e+00
Identity = 741/744 (99.60%), Postives = 743/744 (99.87%), Query Frame = 0

Query: 5   METLMRLIITVVQIGILSAAAATAAAGASQALPGCEEWCGDVQIPYPFGMKEGCYLNEKF 64
           METLMRLIIT VQIGILSAAAATAAAGASQALPGCEEWCGDVQIPYPFGMKEGCYLNEKF
Sbjct: 1   METLMRLIITAVQIGILSAAAATAAAGASQALPGCEEWCGDVQIPYPFGMKEGCYLNEKF 60

Query: 65  FISCNKTHNDSPKAFLQGGNLNVTNISISGELNILHFWARDCYPPNTVPDLSTAFLNTEF 124
           FISCNKTHNDSPKAFLQGGNLNVTNISISGELNILHFWARDCYPPNT+PDLSTAFLNTEF
Sbjct: 61  FISCNKTHNDSPKAFLQGGNLNVTNISISGELNILHFWARDCYPPNTLPDLSTAFLNTEF 120

Query: 125 AVSSAKNKFTVIGCDTFSFISGLVGGTQFYKSACVALCDDITTVKDGACSGNGCCQLEIP 184
           AVSSAKNKFTVIGCDTFSFISGLVGGTQFYKSACVALCDDITTVKDGACSGNGCCQLEIP
Sbjct: 121 AVSSAKNKFTVIGCDTFSFISGLVGGTQFYKSACVALCDDITTVKDGACSGNGCCQLEIP 180

Query: 185 KGLNNLNYTVSSFDNHTNVLSFNPCGYAFVIEEDKFNFSSKYIRHFPLERVPLVLDWAIS 244
           KGLNNLNYTVSSFDNHTNVLSFNPCGYAFVIEEDKFNFSSKYIRHFPLERVPLVLDWAIS
Sbjct: 181 KGLNNLNYTVSSFDNHTNVLSFNPCGYAFVIEEDKFNFSSKYIRHFPLERVPLVLDWAIS 240

Query: 245 NNTCENTTNCICGPSSQKVNFVEDGSEYRCQCSDGFEGNPYLPRGCQDVDECKDGRHDCK 304
           NNTCENTTNCICGPSS+KVNFVEDGSEYRCQCSDGFEGNPYLPRGCQDVDECKDGRHDCK
Sbjct: 241 NNTCENTTNCICGPSSRKVNFVEDGSEYRCQCSDGFEGNPYLPRGCQDVDECKDGRHDCK 300

Query: 305 FECVNTEGNYTCNCPEGFRGDGRREGEGCTRNSKSFVQIIVGVTVGFTVLLIGLTWFYLG 364
           FECVNTEGNYTCNCPEGFRGDGRREGEGCTRNSKSFVQIIVGVTVGFTVLLIGLTWFYLG
Sbjct: 301 FECVNTEGNYTCNCPEGFRGDGRREGEGCTRNSKSFVQIIVGVTVGFTVLLIGLTWFYLG 360

Query: 365 YRKWKFVKLKEEFFEKNGGLMLQQHLSQWQTSADMVRIFTQEELEKATNKYDESAVVGKG 424
           YRKWKFVKLKEEFFEKNGGLMLQQHLSQWQTSADMVRIFTQEELEKATNKYDESAVVGKG
Sbjct: 361 YRKWKFVKLKEEFFEKNGGLMLQQHLSQWQTSADMVRIFTQEELEKATNKYDESAVVGKG 420

Query: 425 GYGTVYKGVLEDGLVVAIKKSKLVDQSQTSQFINEVMVLSQINHRNVVKLLGCCLETQVP 484
           GYGTVYKGVLEDGLVVAIKKSKLVDQSQTSQFINEVMVLSQINHRNVVKLLGCCLETQVP
Sbjct: 421 GYGTVYKGVLEDGLVVAIKKSKLVDQSQTSQFINEVMVLSQINHRNVVKLLGCCLETQVP 480

Query: 485 LLVYEFITNGTLYDHIHDKANHDYSLPWEARLRIASETAGVISYLHSSASTPIIHRDIKT 544
           LLVYEFITNGTLYDHIHDKANHDYSLPWEARLRIASETAGVISYLHSSASTPIIHRDIKT
Sbjct: 481 LLVYEFITNGTLYDHIHDKANHDYSLPWEARLRIASETAGVISYLHSSASTPIIHRDIKT 540

Query: 545 TNILLDQNYTAKVSDFGASKLVPLDQTQLSTMVQGTLGYLDPEYLLTSELTEKSDVYSFG 604
           TNILLDQNYTAKVSDFGASKLVPLDQTQLSTMVQGTLGYLDPEYLLTSELTEKSDVYSFG
Sbjct: 541 TNILLDQNYTAKVSDFGASKLVPLDQTQLSTMVQGTLGYLDPEYLLTSELTEKSDVYSFG 600

Query: 605 IVLMELITGKKAVSFEGPEAERNLAMYVLCAMKEDRLEEVVEKGMAREGKLEQIKEVAKV 664
           IVLMELITGKKAVSFEGPEAERNLAMYVLCAMKEDRLEEVVEKGMAREGKLEQIKEVAKV
Sbjct: 601 IVLMELITGKKAVSFEGPEAERNLAMYVLCAMKEDRLEEVVEKGMAREGKLEQIKEVAKV 660

Query: 665 AKECLRVRGEERPSMKEVAMELEGLRVLVENKWVNDNNLINAEEMVAYLLEDNNGASEST 724
           AKECLRVRGEERPSMKEVAMELEGLRVLVENKWVNDNNLINAEEMVAYLLEDNNGASEST
Sbjct: 661 AKECLRVRGEERPSMKEVAMELEGLRVLVENKWVNDNNLINAEEMVAYLLEDNNGASEST 720

Query: 725 LLSMNNVVDDSMKVQILPRIHDGR 749
           LLSMNNVVDDSMKVQILPRIHDGR
Sbjct: 721 LLSMNNVVDDSMKVQILPRIHDGR 744

BLAST of MS015728 vs. NCBI nr
Match: XP_023512416.1 (wall-associated receptor kinase 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1130.2 bits (2922), Expect = 0.0e+00
Identity = 572/756 (75.66%), Postives = 641/756 (84.79%), Query Frame = 0

Query: 1   LRRPMETLMRLIITVVQIGILSAAAATAAAGASQALPGCEEWCGDVQIPYPFGMKEGCYL 60
           + R +ETL+RL+I  V I ILS+        ASQAL GC + CGD+QIPYPFG +EGCYL
Sbjct: 1   MERQIETLIRLMI--VNILILSSPVY-----ASQALLGCPDRCGDLQIPYPFGTREGCYL 60

Query: 61  NEKFFISCNKTHNDSPKAFLQGGNLNVTNISISGELNILHFWARDCYPPNTVPDL--STA 120
           N+ F I+CN TH D P+ FL+ GN+ VTNISISGEL ILHF A+DCYP N   D   ++A
Sbjct: 61  NKNFLITCNTTHFDPPRPFLRTGNIQVTNISISGELQILHFGAKDCYPKNNSVDARRTSA 120

Query: 121 FLN-TEFAVSSAKNKFTVIGCDTFSFISGLVGGTQFYKSACVALCDDITTVKDGACSGNG 180
            LN + F VSS KNKFTVIGCDT++F+SG + G Q Y++ACVALCD+ITTV+DGACSGNG
Sbjct: 121 TLNLSTFTVSSTKNKFTVIGCDTYAFLSGQIEG-QSYRTACVALCDNITTVRDGACSGNG 180

Query: 181 CCQLEIPKGLNNLNYTVSSFDNHTNVLSFNPCGYAFVIEEDKFNFSSKYIRHFPLERVPL 240
           CCQL+IP GL  L Y V SFDNHT+VLSFNPCGYAFV EEDKF+FS+ YIR FP  +VP+
Sbjct: 181 CCQLDIPSGLKGLRYRVRSFDNHTDVLSFNPCGYAFVTEEDKFHFSAAYIRDFPQRQVPV 240

Query: 241 VLDWAISNNTCENT---TNCICGPSSQKVNFVEDGSEYRCQCSDGFEGNPYLPRGCQDVD 300
           VLDW ISN TC      +NC+CGP+S  VN + DGSEYRC C DGFEGNPYLPRGCQD+D
Sbjct: 241 VLDWGISNTTCSTANKKSNCVCGPNSMMVNPLPDGSEYRCGCLDGFEGNPYLPRGCQDID 300

Query: 301 ECKDGR-HDCKFECVNTEGNYTCNCPEGFRGDGRREGEGCTRNSKSFVQIIVGVTVGFTV 360
           EC+D R +DCKFECVNTEGNYTCNCPEGF+GDGRR GEGCTR+SKSFVQ+I+GV+VGFTV
Sbjct: 301 ECRDERLNDCKFECVNTEGNYTCNCPEGFKGDGRRGGEGCTRSSKSFVQVIIGVSVGFTV 360

Query: 361 LLIGLTWFYLGYRKWKFVKLKEEFFEKNGGLMLQQHLSQWQTSADMVRIFTQEELEKATN 420
           L+IG TW YLGYRKWK +KLKE+FFE+NGGLMLQ+HLSQW++S DMV IFTQEEL+KATN
Sbjct: 361 LVIGSTWLYLGYRKWKLIKLKEKFFEENGGLMLQRHLSQWKSSTDMVTIFTQEELDKATN 420

Query: 421 KYDESAVVGKGGYGTVYKGVLEDGLVVAIKKSKLVDQSQTSQFINEVMVLSQINHRNVVK 480
           KYDESAV+GKGGYGTVYKG+L DG VVAIKKSKLVDQSQTSQFINEV+VLSQINHRNVVK
Sbjct: 421 KYDESAVIGKGGYGTVYKGILPDGSVVAIKKSKLVDQSQTSQFINEVIVLSQINHRNVVK 480

Query: 481 LLGCCLETQVPLLVYEFITNGTLYDHIHDKANHDYSLPWEARLRIASETAGVISYLHSSA 540
           LLGCCLETQVPLLVYEF+TNGTL+DHIHD   H   L WEARLRIASETAGVISYLHSSA
Sbjct: 481 LLGCCLETQVPLLVYEFVTNGTLFDHIHDTTKH-VPLSWEARLRIASETAGVISYLHSSA 540

Query: 541 STPIIHRDIKTTNILLDQNYTAKVSDFGASKLVPLDQTQLSTMVQGTLGYLDPEYLLTSE 600
           STPIIHRDIKTTNILLD NY AKVSDFGASKLV LDQTQLSTMVQGTLGYLDPEYLLTSE
Sbjct: 541 STPIIHRDIKTTNILLDDNYNAKVSDFGASKLVTLDQTQLSTMVQGTLGYLDPEYLLTSE 600

Query: 601 LTEKSDVYSFGIVLMELITGKKAVSFEGPEAERNLAMYVLCAMKEDRLEEVVEKGMAREG 660
           LTEKSDVYSFGIVL+ELITGKKAVSFEGPEAERNLAMYVLCAMKEDRL EVVEKGMAREG
Sbjct: 601 LTEKSDVYSFGIVLLELITGKKAVSFEGPEAERNLAMYVLCAMKEDRLGEVVEKGMAREG 660

Query: 661 KLEQIKEVAKVAKECLRVRGEERPSMKEVAMELEGLRVLVENKWVNDNNLINAEEMVAYL 720
           +  QIKEV KVA++CLR+ GEERPSMKEVAMELEGLRV+VE++WVN+ NL+         
Sbjct: 661 EFGQIKEVGKVARKCLRISGEERPSMKEVAMELEGLRVMVEHEWVNEENLV--------- 720

Query: 721 LEDNNGASESTLLS-MNNVVDDSMKVQILPRIHDGR 749
               +GAS   ++S   NVVDDSMKVQILP IHDGR
Sbjct: 721 ---ADGASNMFVVSGSTNVVDDSMKVQILPLIHDGR 735

BLAST of MS015728 vs. NCBI nr
Match: XP_022960687.1 (wall-associated receptor kinase 3-like [Cucurbita moschata])

HSP 1 Score: 1121.7 bits (2900), Expect = 0.0e+00
Identity = 569/755 (75.36%), Postives = 641/755 (84.90%), Query Frame = 0

Query: 3    RPMETLMRLIITVVQIGILSAAAATAAAGASQALPGCEEWCGDVQIPYPFGMKEGCYLNE 62
            R +ETL+RL+I  V I ILS+        ASQAL GC + CGD+QIPYPFG +EGCYLN+
Sbjct: 710  RQIETLIRLMI--VNILILSSPVY-----ASQALLGCPDRCGDLQIPYPFGTREGCYLNK 769

Query: 63   KFFISCNKTHNDSPKAFLQGGNLNVTNISISGELNILHFWARDCYPPNTVPDL--STAFL 122
             F I+CN TH + P+ FL+ GN+ VTNISISGEL ILHF A+DCYP N   D   ++A L
Sbjct: 770  NFLITCNTTHFNPPRPFLRTGNIQVTNISISGELQILHFGAKDCYPKNNSLDARRTSATL 829

Query: 123  N-TEFAVSSAKNKFTVIGCDTFSFISGLVGGTQFYKSACVALCDDITTVKDGACSGNGCC 182
            N + F VSS KNKFTVIGCDT++F+SG + G Q Y++ACVALCD+ITTV+DGACSGNGCC
Sbjct: 830  NLSTFTVSSTKNKFTVIGCDTYAFLSGQIEG-QSYRTACVALCDNITTVRDGACSGNGCC 889

Query: 183  QLEIPKGLNNLNYTVSSFDNHTNVLSFNPCGYAFVIEEDKFNFSSKYIRHFPLERVPLVL 242
            QL+IP GL  L Y V SFDNHT+VLSFNPCGYAFV EEDKF+FS+ YIR FP  +VP+VL
Sbjct: 890  QLDIPSGLKGLRYRVRSFDNHTDVLSFNPCGYAFVTEEDKFHFSAAYIRDFPQRQVPVVL 949

Query: 243  DWAISNNTC---ENTTNCICGPSSQKVNFVEDGSEYRCQCSDGFEGNPYLPRGCQDVDEC 302
            DW ISN TC    N +NCICGP+S  VN + DGSEYRC+C DGFEGNPYLPRGCQD+DEC
Sbjct: 950  DWGISNTTCSTANNKSNCICGPNSMMVNPLHDGSEYRCRCLDGFEGNPYLPRGCQDIDEC 1009

Query: 303  KDGR-HDCKFECVNTEGNYTCNCPEGFRGDGRREGEGCTRNSKSFVQIIVGVTVGFTVLL 362
            +D R +DCKFECVNT+GNYTCNCPEGF+GDGRR GEGCTR+SKSFVQ+I+GV+VGFTVL+
Sbjct: 1010 RDERLNDCKFECVNTKGNYTCNCPEGFKGDGRRGGEGCTRSSKSFVQVIIGVSVGFTVLV 1069

Query: 363  IGLTWFYLGYRKWKFVKLKEEFFEKNGGLMLQQHLSQWQTSADMVRIFTQEELEKATNKY 422
            IG TW YLGYRKWK +KLKE+FFE+NGGLMLQ+HLSQW++S D V IFTQEEL+KATNKY
Sbjct: 1070 IGSTWLYLGYRKWKLIKLKEKFFEENGGLMLQRHLSQWKSSTDTVTIFTQEELDKATNKY 1129

Query: 423  DESAVVGKGGYGTVYKGVLEDGLVVAIKKSKLVDQSQTSQFINEVMVLSQINHRNVVKLL 482
            DESAV+GKGGYGTVYKG L DG VVAIKKSKLVDQSQTSQFINEV+VLSQINHRNVVKLL
Sbjct: 1130 DESAVIGKGGYGTVYKGFLPDGSVVAIKKSKLVDQSQTSQFINEVIVLSQINHRNVVKLL 1189

Query: 483  GCCLETQVPLLVYEFITNGTLYDHIHDKANHDYSLPWEARLRIASETAGVISYLHSSAST 542
            GCCLETQVPLLVYEF+TNGTL+DHIHD   H   L WEARLRIASETAGVISYLHSSAST
Sbjct: 1190 GCCLETQVPLLVYEFVTNGTLFDHIHDTTKH-VPLSWEARLRIASETAGVISYLHSSAST 1249

Query: 543  PIIHRDIKTTNILLDQNYTAKVSDFGASKLVPLDQTQLSTMVQGTLGYLDPEYLLTSELT 602
            PIIHRDIKTTNILLD NY AKVSDFGASKLVPLDQTQLSTMVQGTLGYLDPEYLLTSELT
Sbjct: 1250 PIIHRDIKTTNILLDDNYNAKVSDFGASKLVPLDQTQLSTMVQGTLGYLDPEYLLTSELT 1309

Query: 603  EKSDVYSFGIVLMELITGKKAVSFEGPEAERNLAMYVLCAMKEDRLEEVVEKG-MAREGK 662
            EKSDVYSFGIVL+ELITGKKAVSFEGPEAERNLAMYVLCAMKEDRL +VVEKG MARE +
Sbjct: 1310 EKSDVYSFGIVLLELITGKKAVSFEGPEAERNLAMYVLCAMKEDRLGDVVEKGIMARERE 1369

Query: 663  LEQIKEVAKVAKECLRVRGEERPSMKEVAMELEGLRVLVENKWVNDNNLINAEEMVAYLL 722
             EQIKEV KVA++CLR+ GEERPSMKEVAMELEGLRV+VE++WVN+ N++          
Sbjct: 1370 FEQIKEVGKVARKCLRISGEERPSMKEVAMELEGLRVMVEHQWVNEENMV---------- 1429

Query: 723  EDNNGASESTLLS-MNNVVDDSMKVQILPRIHDGR 749
               +GAS   ++S   NVVDDSMKVQ+LP IHDGR
Sbjct: 1430 --ADGASHMFVVSGSTNVVDDSMKVQVLPLIHDGR 1443

BLAST of MS015728 vs. NCBI nr
Match: XP_022989770.1 (wall-associated receptor kinase 2-like [Cucurbita maxima])

HSP 1 Score: 1120.9 bits (2898), Expect = 0.0e+00
Identity = 564/754 (74.80%), Postives = 640/754 (84.88%), Query Frame = 0

Query: 3   RPMETLMRLIITVVQIGILSAAAATAAAGASQALPGCEEWCGDVQIPYPFGMKEGCYLNE 62
           R ++T +RL+I  + I ILS    +  A ASQAL GC++ CGD+QIPYPFG +EGCYLN+
Sbjct: 3   RQIKTHIRLMI--INILILS---PSVYAVASQALLGCDDRCGDLQIPYPFGTREGCYLNK 62

Query: 63  KFFISCNKTHNDSPKAFLQGGNLNVTNISISGELNILHFWARDCYPPNTVPDL--STAFL 122
            F I+CN TH D P+ FL+ GN+ VTNISISGEL ILHF A+DCYP N   D   ++A L
Sbjct: 63  NFLITCNTTHFDPPRPFLRTGNIQVTNISISGELQILHFGAKDCYPKNNSLDARRTSATL 122

Query: 123 N-TEFAVSSAKNKFTVIGCDTFSFISGLVGGTQFYKSACVALCDDITTVKDGACSGNGCC 182
           N + F VSS KNKFTVIGCDT++F+SG + G Q Y++ACVALCD+ITTV+DGACSGNGCC
Sbjct: 123 NLSTFTVSSTKNKFTVIGCDTYAFLSGQIEG-QSYRTACVALCDNITTVRDGACSGNGCC 182

Query: 183 QLEIPKGLNNLNYTVSSFDNHTNVLSFNPCGYAFVIEEDKFNFSSKYIRHFPLERVPLVL 242
           QL+IP GL  L Y V SFDNHT+V SFNPCGYAFV EEDKF+FS+ YIR+FP  +VP+VL
Sbjct: 183 QLDIPSGLKGLRYRVRSFDNHTDVFSFNPCGYAFVTEEDKFHFSAAYIRNFPQRQVPVVL 242

Query: 243 DWAISNNTC---ENTTNCICGPSSQKVNFVEDGSEYRCQCSDGFEGNPYLPRGCQDVDEC 302
           DW ISN TC    N +NCICGP+S  VN + DGSEYRC C DGFEGNPYLPRGCQD+DEC
Sbjct: 243 DWGISNTTCSTANNKSNCICGPNSMMVNVLPDGSEYRCGCLDGFEGNPYLPRGCQDIDEC 302

Query: 303 KDGR-HDCKFECVNTEGNYTCNCPEGFRGDGRREGEGCTRNSKSFVQIIVGVTVGFTVLL 362
           +D R +DCKFECVNT+GNYTCNCPEGF+GDGRR GEGCTR+SKSFVQ+I+GV+VGFTVL+
Sbjct: 303 RDERLNDCKFECVNTKGNYTCNCPEGFKGDGRRGGEGCTRSSKSFVQVIIGVSVGFTVLV 362

Query: 363 IGLTWFYLGYRKWKFVKLKEEFFEKNGGLMLQQHLSQWQTSADMVRIFTQEELEKATNKY 422
           IG TW YLGYRKWK +KLKE+FFE+NGGLMLQ+HLSQW++S D V IFTQEEL+KATNKY
Sbjct: 363 IGSTWLYLGYRKWKLIKLKEKFFEENGGLMLQRHLSQWKSSTDTVTIFTQEELDKATNKY 422

Query: 423 DESAVVGKGGYGTVYKGVLEDGLVVAIKKSKLVDQSQTSQFINEVMVLSQINHRNVVKLL 482
           DESAV+GKGGYGTVYKG+L DG VVAIKKSKLVDQSQTSQFINEV+VLSQINHRNVVKLL
Sbjct: 423 DESAVIGKGGYGTVYKGILPDGSVVAIKKSKLVDQSQTSQFINEVIVLSQINHRNVVKLL 482

Query: 483 GCCLETQVPLLVYEFITNGTLYDHIHDKANHDYSLPWEARLRIASETAGVISYLHSSAST 542
           GCCLETQVPLLVYEF+TNGTL+DHIHD   H   L W+ARLRIA ETAGVISYLHSSAST
Sbjct: 483 GCCLETQVPLLVYEFVTNGTLFDHIHDTTKH-VPLSWKARLRIALETAGVISYLHSSAST 542

Query: 543 PIIHRDIKTTNILLDQNYTAKVSDFGASKLVPLDQTQLSTMVQGTLGYLDPEYLLTSELT 602
           PIIHRDIKTTNILLD NY AKVSDFGASKLVPLDQTQLSTMVQGTLGYLDPEYLLTSELT
Sbjct: 543 PIIHRDIKTTNILLDDNYNAKVSDFGASKLVPLDQTQLSTMVQGTLGYLDPEYLLTSELT 602

Query: 603 EKSDVYSFGIVLMELITGKKAVSFEGPEAERNLAMYVLCAMKEDRLEEVVEKGMAREGKL 662
           EKSDVYSFGIVL+ELITGKKAVSFEGPEAERNLAMYVLCAMKEDRL EVVEKGMAREG+ 
Sbjct: 603 EKSDVYSFGIVLLELITGKKAVSFEGPEAERNLAMYVLCAMKEDRLGEVVEKGMAREGEF 662

Query: 663 EQIKEVAKVAKECLRVRGEERPSMKEVAMELEGLRVLVENKWVNDNNLINAEEMVAYLLE 722
           EQIK+V KVA++CLR+ GEERPSMKEV MELEGLRV+ E++WV++ NL+           
Sbjct: 663 EQIKDVGKVARKCLRISGEERPSMKEVVMELEGLRVMAEHEWVDEENLV----------- 722

Query: 723 DNNGASESTLLS-MNNVVDDSMKVQILPRIHDGR 749
             +GAS   ++S   NVVDDSMKVQ+LP IHDGR
Sbjct: 723 -ADGASNIFVVSGSTNVVDDSMKVQVLPLIHDGR 737

BLAST of MS015728 vs. NCBI nr
Match: XP_038884307.1 (wall-associated receptor kinase 2-like [Benincasa hispida])

HSP 1 Score: 1109.4 bits (2868), Expect = 0.0e+00
Identity = 560/762 (73.49%), Postives = 636/762 (83.46%), Query Frame = 0

Query: 3   RPMETLMR------LIITVVQIGILSAAAATAAAGASQALPGCEEWCGDVQIPYPFGMKE 62
           RP ETL+R      L I +    +++A   T A  ASQALPGC+EWCGD+QIPYPFG +E
Sbjct: 3   RPTETLIRLTAVKILAILLSAFVVVTATTNTTATVASQALPGCDEWCGDLQIPYPFGTRE 62

Query: 63  GCYLNEKFFISCNKTHNDSPKAFLQGGNLNVTNISISGELNILHFWARDCYPPNTVPDL- 122
           GCYLNE+FFI+C+ T  DSPKAFL+GG++ VTNISISGEL IL+  A++C+PP   PD+ 
Sbjct: 63  GCYLNERFFINCSTTRQDSPKAFLRGGSVPVTNISISGELQILNLVAQECHPPKIGPDVF 122

Query: 123 STAFLNTEFAVSSAKNKFTVIGCDTFSFISGLVGGTQFYKSACVALCDDITTVKDGACSG 182
           S  FL+  + +S  KNKF  +GC+TF+ I G     Q +++ACVALCDDI +VKDGACSG
Sbjct: 123 SNVFLDLSYIISRTKNKFIAMGCNTFAHIFGRDIKGQSFRTACVALCDDIGSVKDGACSG 182

Query: 183 NGCCQLEIPKGLNNLNYTVSSFDNHTNVLSFNPCGYAFVIEEDKFNFSSKYIRHFPLERV 242
           NGCCQLEIP  LN+L +TVSS +N  NV SFNPCGYAF++E++ FNFSSKYIR+FP  RV
Sbjct: 183 NGCCQLEIPSNLNHLEFTVSSLNNDVNVSSFNPCGYAFLVEQNSFNFSSKYIRNFPENRV 242

Query: 243 PLVLDWAISNNTC---ENTTNCICGPSSQKVNFVEDGSEYRCQCSDGFEGNPYLPRGCQD 302
             VLDWAISN+TC   EN TNCICG +S KV+ ++D S YRCQC +GFEGNPYLP GCQD
Sbjct: 243 LAVLDWAISNDTCVTAENKTNCICGRNSTKVDLLDDPSRYRCQCLEGFEGNPYLPEGCQD 302

Query: 303 VDECKD-GRHDCKFECVNTEGNYTCNCPEGFRGDGRREGEGCTRNSKSFVQIIVGVTVGF 362
           +DECKD   +DC FECVN  GNYTCNCP+GF+GDGRR GEGCTR SKSFVQIIVG+TVGF
Sbjct: 303 IDECKDESLNDCSFECVNRIGNYTCNCPKGFKGDGRRGGEGCTRESKSFVQIIVGLTVGF 362

Query: 363 TVLLIGLTWFYLGYRKWKFVKLKEEFFEKNGGLMLQQHLSQWQTSADMVRIFTQEELEKA 422
            VL+I  TW YLGYRKWKF+KLKE+FFEKNGGLMLQQHLSQWQTS DMVRIFT+EEL+KA
Sbjct: 363 AVLVIASTWLYLGYRKWKFIKLKEKFFEKNGGLMLQQHLSQWQTSTDMVRIFTREELDKA 422

Query: 423 TNKYDESAVVGKGGYGTVYKGVLEDGLVVAIKKSKLVDQSQTSQFINEVMVLSQINHRNV 482
           TNKYDESAVVGKGGYGTVYKGVL+DG VVAIKKSKLVDQSQTSQFINEV+VLSQINHRNV
Sbjct: 423 TNKYDESAVVGKGGYGTVYKGVLDDGSVVAIKKSKLVDQSQTSQFINEVIVLSQINHRNV 482

Query: 483 VKLLGCCLETQVPLLVYEFITNGTLYDHIHDKANHDYSLPWEARLRIASETAGVISYLHS 542
           VKLLGCCLET+VPLLVYEFITNGTLY+++HDK +  ++L WEARLRIASETAGVISYLHS
Sbjct: 483 VKLLGCCLETEVPLLVYEFITNGTLYEYVHDKTSDRHTLSWEARLRIASETAGVISYLHS 542

Query: 543 SASTPIIHRDIKTTNILLDQNYTAKVSDFGASKLVPLDQTQLSTMVQGTLGYLDPEYLLT 602
           SASTPIIHRDIKTTNILLD NYTAKVSDFGASKLVP+DQTQLSTMVQGTLGYLDPEYLLT
Sbjct: 543 SASTPIIHRDIKTTNILLDHNYTAKVSDFGASKLVPVDQTQLSTMVQGTLGYLDPEYLLT 602

Query: 603 SELTEKSDVYSFGIVLMELITGKKAVSFEGPEAERNLAMYVLCAMKEDRLEEVVEKGMAR 662
           SELTEKSDVYSFGIVL+ELITGKKAVSFEG E ERNLAMYV+CAMKEDRL EVVEKG+A 
Sbjct: 603 SELTEKSDVYSFGIVLLELITGKKAVSFEGSEVERNLAMYVMCAMKEDRLTEVVEKGIAT 662

Query: 663 EGKLEQIKEVAKVAKECLRVRGEERPSMKEVAMELEGLRVLVENKWVNDNNLINAEEMVA 722
           E   EQIK+VAK+A +CLR+RGEERPSMKEVAMELEGLR L+E    N+      E MV 
Sbjct: 663 EANFEQIKQVAKLATKCLRIRGEERPSMKEVAMELEGLRGLIE---ANEKLGSTGEGMVG 722

Query: 723 YLLEDNNGASES-----TLLSMNNVVDDSMKVQILPRIHDGR 749
           YL+  +NGASES     T     N+VDDSMKV ILP IH+GR
Sbjct: 723 YLV-PSNGASESIFNQFTASGSTNIVDDSMKVHILPLIHNGR 760

BLAST of MS015728 vs. ExPASy Swiss-Prot
Match: Q9LMP1 (Wall-associated receptor kinase 2 OS=Arabidopsis thaliana OX=3702 GN=WAK2 PE=1 SV=1)

HSP 1 Score: 617.1 bits (1590), Expect = 2.7e-175
Identity = 326/675 (48.30%), Postives = 451/675 (66.81%), Query Frame = 0

Query: 39  CEEWCGDVQIPYPFGMKEGCYL--NEKFFISCNKTHNDSPKAFLQGGNLNVTNISISGEL 98
           C+  CG+V + YPFG   GCY   +E F ++C    N+  K F   GN+ V N+S+SG+L
Sbjct: 29  CQTRCGNVAVEYPFGTSPGCYYPGDESFNLTC----NEQEKLFF--GNMPVINMSLSGQL 88

Query: 99  NILHFWARDCYPPNTVPDLSTAFLNT--EFAVSSAKNKFTVIGCDTFSFISGLVGGTQFY 158
            +    +R CY          A   T   F +S   N+FTV+GC++++F+     G + Y
Sbjct: 89  RVRLVRSRVCYDSQGKQTDYIAQRTTLGNFTLSEL-NRFTVVGCNSYAFLR--TSGVEKY 148

Query: 159 KSACVALCDDITTVKDGACSGNGCCQLEIPKGLNNLNYTVSSFDNHTNVLSFNPCGYAFV 218
            + C+++CD  TT K+G+CSG GCCQ+ +P+G + +     SF NH  V  FNPC YAF+
Sbjct: 149 STGCISICDSATT-KNGSCSGEGCCQIPVPRGYSFVRVKPHSFHNHPTVHLFNPCTYAFL 208

Query: 219 IEEDKFNFSS----KYIRHFPLERVPLVLDWAISNNTCENTT-NCICGPSSQKVNFVEDG 278
           +E+  F+F +      +R+  +   P+VLDW+I + TC+      +CG +S   +    G
Sbjct: 209 VEDGMFDFHALEDLNNLRN--VTTFPVVLDWSIGDKTCKQVEYRGVCGGNSTCFDST-GG 268

Query: 279 SEYRCQCSDGFEGNPYLPRGCQDVDECKDGRHDCK--FECVNTEGNYTCNCPEGFRGDGR 338
           + Y C+C +GFEGNPYLP GCQD++EC   RH+C     C NT+G++ CNCP G+R D  
Sbjct: 269 TGYNCKCLEGFEGNPYLPNGCQDINECISSRHNCSEHSTCENTKGSFNCNCPSGYRKDSL 328

Query: 339 REGEGCTRNSK----SFVQIIVGVTVGFTVLLIGLTWFYLGYRKWKFVKLKEEFFEKNGG 398
                CTR  +     + QI +G T+GF+V+++G++      +  K  +L+++FFE+NGG
Sbjct: 329 ---NSCTRKVRPEYFRWTQIFLGTTIGFSVIMLGISCLQQKIKHRKNTELRQKFFEQNGG 388

Query: 399 LMLQQHLSQWQTSADMVRIFTQEELEKATNKYDESAVVGKGGYGTVYKGVLEDGLVVAIK 458
            ML Q +S    S   V+IFT++ +++ATN Y ES ++G+GG GTVYKG+L D  +VAIK
Sbjct: 389 GMLIQRVSGAGPSNVDVKIFTEKGMKEATNGYHESRILGQGGQGTVYKGILPDNSIVAIK 448

Query: 459 KSKLVDQSQTSQFINEVMVLSQINHRNVVKLLGCCLETQVPLLVYEFITNGTLYDHIHDK 518
           K++L ++SQ  QFINEV+VLSQINHRNVVK+LGCCLET+VPLLVYEFI +GTL+DH+H  
Sbjct: 449 KARLGNRSQVEQFINEVLVLSQINHRNVVKVLGCCLETEVPLLVYEFINSGTLFDHLHGS 508

Query: 519 ANHDYSLPWEARLRIASETAGVISYLHSSASTPIIHRDIKTTNILLDQNYTAKVSDFGAS 578
             +D SL WE RLRIA+E AG ++YLHSSAS PIIHRDIKT NILLD+N TAKV+DFGAS
Sbjct: 509 L-YDSSLTWEHRLRIATEVAGSLAYLHSSASIPIIHRDIKTANILLDKNLTAKVADFGAS 568

Query: 579 KLVPLDQTQLSTMVQGTLGYLDPEYLLTSELTEKSDVYSFGIVLMELITGKKAVSFEGPE 638
           +L+P+D+ QL+T+VQGTLGYLDPEY  T  L EKSDVYSFG+VLMEL++G+KA+ FE P 
Sbjct: 569 RLIPMDKEQLTTIVQGTLGYLDPEYYNTGLLNEKSDVYSFGVVLMELLSGQKALCFERPH 628

Query: 639 AERNLAMYVLCAMKEDRLEEVVEKGMAREGKLEQIKEVAKVAKECLRVRGEERPSMKEVA 698
             +NL      A K +R  E+++  +  E    +I+E A++A EC R+ GEERP MKEVA
Sbjct: 629 CPKNLVSCFASATKNNRFHEIIDGQVMNEDNQREIQEAARIAAECTRLMGEERPRMKEVA 686

BLAST of MS015728 vs. ExPASy Swiss-Prot
Match: Q9LMN7 (Wall-associated receptor kinase 5 OS=Arabidopsis thaliana OX=3702 GN=WAK5 PE=2 SV=1)

HSP 1 Score: 599.4 bits (1544), Expect = 5.7e-170
Identity = 330/708 (46.61%), Postives = 450/708 (63.56%), Query Frame = 0

Query: 33  SQALPGCEEWCGDVQIPYPFGMKEGCYL--NEKFFISCNKTHNDSPKAFLQGGNLNVTNI 92
           +Q    C+  CGDV I YPFG+  GCY   ++ F I+C +   D P       N+ V N 
Sbjct: 23  AQPRDDCQTRCGDVPIDYPFGISTGCYYPGDDSFNITCEE---DKPNVL---SNIEVLNF 82

Query: 93  SISGELNILHFWARDCYPPNTVPDLSTAFLNTEFAVSSAKNKFTVIGCDTFSFISGLVGG 152
           + SG+L  L   +  CY   T  D  + +   +    S  NKFT++GC+ ++ +S    G
Sbjct: 83  NHSGQLRGLIPRSTVCYDQQTNNDFESLWFRLDNLSFSPNNKFTLVGCNAWALLSTF--G 142

Query: 153 TQFYKSACVALCDDITTVKDGACSGNGCCQLE--IPKGLNNLNYTVSSFDNHTNVLSFNP 212
            Q Y + C++LC D     +  C+G GCC+ E  IP   + +    S F+N T+V  FNP
Sbjct: 143 IQNYSTGCMSLC-DTPPPPNSKCNGVGCCRTEVSIPLDSHRIETQPSRFENMTSVEHFNP 202

Query: 213 CGYAFVIEEDKFNFSS----KYIRHFPLERVPLVLDWAISNNTCENTT-NCICGPSSQKV 272
           C YAF +E+  FNFSS    K +R+  + R P++LDW+I N TCE      ICG +S   
Sbjct: 203 CSYAFFVEDGMFNFSSLEDLKDLRN--VTRFPVLLDWSIGNQTCEQVVGRNICGGNSTCF 262

Query: 273 NFVEDGSEYRCQCSDGFEGNPYLPRGCQDVDECKDGRHDCK--FECVNTEGNYTCNCPEG 332
           +    G  Y C+C  GF+GNPYL  GCQD++EC    H+C     C NT G++ C CP G
Sbjct: 263 DSTR-GKGYNCKCLQGFDGNPYLSDGCQDINECTTRIHNCSDTSTCENTLGSFHCQCPSG 322

Query: 333 FRGDGRREGEGCTRNSK------SFVQIIVGVTVGFTVLLIGLTWFYLGYRKWKFVKLKE 392
              D       C    K       +  +++G T+GF ++L+ +++     R  K  +L++
Sbjct: 323 --SDLNTTTMSCIDTPKEEPKYLGWTTVLLGTTIGFLIILLTISYIQQKMRHRKNTELRQ 382

Query: 393 EFFEKNGGLMLQQHLSQWQTSADMVRIFTQEELEKATNKYDESAVVGKGGYGTVYKGVLE 452
           +FFE+NGG ML Q LS    S   V+IFT+E +++AT+ Y+ES ++G+GG GTVYKG+L+
Sbjct: 383 QFFEQNGGGMLIQRLSGAGPSNVDVKIFTEEGMKEATDGYNESRILGQGGQGTVYKGILQ 442

Query: 453 DGLVVAIKKSKLVDQSQTSQFINEVMVLSQINHRNVVKLLGCCLETQVPLLVYEFITNGT 512
           D  +VAIKK++L D+SQ  QFINEV+VLSQINHRNVVKLLGCCLET+VPLLVYEFI++GT
Sbjct: 443 DNSIVAIKKARLGDRSQVEQFINEVLVLSQINHRNVVKLLGCCLETEVPLLVYEFISSGT 502

Query: 513 LYDHIHDKANHDYSLPWEARLRIASETAGVISYLHSSASTPIIHRDIKTTNILLDQNYTA 572
           L+DH+H  +  D SL WE RLRIA E AG ++YLHS AS PIIHRD+KT NILLD+N TA
Sbjct: 503 LFDHLHG-SMFDSSLTWEHRLRIAIEVAGTLAYLHSYASIPIIHRDVKTANILLDENLTA 562

Query: 573 KVSDFGASKLVPLDQTQLSTMVQGTLGYLDPEYLLTSELTEKSDVYSFGIVLMELITGKK 632
           KV+DFGAS+L+P+DQ QL+TMVQGTLGYLDPEY  T  L EKSDVYSFG+VLMEL++G+K
Sbjct: 563 KVADFGASRLIPMDQEQLTTMVQGTLGYLDPEYYNTGLLNEKSDVYSFGVVLMELLSGEK 622

Query: 633 AVSFEGPEAERNLAMYVLCAMKEDRLEEVVEKGMAREGKLEQIKEVAKVAKECLRVRGEE 692
           A+ FE P++ ++L  Y + AMKE+RL E+++  +  E    +I+E A++A EC R+ GEE
Sbjct: 623 ALCFERPQSSKHLVSYFVSAMKENRLHEIIDGQVMNEYNQREIQESARIAVECTRIMGEE 682

Query: 693 RPSMKEVAMELEGLRVLVENKWVNDNNLINAEEMVAYLLEDNNGASES 724
           RPSMKEVA ELE LRV       +D      E ++   +    G + S
Sbjct: 683 RPSMKEVAAELEALRVKTTKHQWSDQYPKEVEHLLGVQILSTQGDTSS 715

BLAST of MS015728 vs. ExPASy Swiss-Prot
Match: Q39191 (Wall-associated receptor kinase 1 OS=Arabidopsis thaliana OX=3702 GN=WAK1 PE=1 SV=2)

HSP 1 Score: 597.0 bits (1538), Expect = 2.8e-169
Identity = 331/716 (46.23%), Postives = 449/716 (62.71%), Query Frame = 0

Query: 22  SAAAATAAAGASQALPGCEEWCGDVQIPYPFGMKEGCYL--NEKFFISCNKTHNDSPKAF 81
           S A      G  Q    C+  CG++ I YPFG+  GCY   NE F I+C +   D P   
Sbjct: 15  SLACTQLVKGQHQPGENCQNKCGNITIEYPFGISSGCYYPGNESFSITCKE---DRPHVL 74

Query: 82  LQGGNLNVTNISISGELNILHFWARDCYPPNTVPDLSTAFLNTEFAVSSAKNKFTVIGCD 141
               ++ V N + SG+L +L   +  CY          +    E    SA NK T +GC+
Sbjct: 75  ---SDIEVANFNHSGQLQVLLNRSSTCYDEQGKKTEEDSSFTLENLSLSANNKLTAVGCN 134

Query: 142 TFSFISGLVGGTQFYKSACVALCDDITTVKDGACSGNGCCQLEIPKGLNNLNYTVSS--F 201
             S +     G Q Y +AC++LCD      DG C+G GCC++++   L++  +  +S   
Sbjct: 135 ALSLLDTF--GMQNYSTACLSLCDS-PPEADGECNGRGCCRVDVSAPLDSYTFETTSGRI 194

Query: 202 DNHTNVLSFNPCGYAFVIEEDKFNFSS--KYIRHFPLERVPLVLDWAISNNTCENT-TNC 261
            + T+   F+PC YAF++E+DKFNFSS    +    + R P++LDW++ N TCE   +  
Sbjct: 195 KHMTSFHDFSPCTYAFLVEDDKFNFSSTEDLLNLRNVMRFPVLLDWSVGNQTCEQVGSTS 254

Query: 262 ICGPSSQKVNFVEDGSEYRCQCSDGFEGNPYLPRGCQDVDECKDG----RHDCK--FECV 321
           ICG +S  ++       Y C+C++GF+GNPYL  GCQDV+EC       RH+C     C 
Sbjct: 255 ICGGNSTCLDSTPRNG-YICRCNEGFDGNPYLSAGCQDVNECTTSSTIHRHNCSDPKTCR 314

Query: 322 NTEGNYTCNCPEGFRGDGRREGEGCTRNSKSFVQIIVGVTVGFTVLLIGLTWFYLGYRKW 381
           N  G + C C  G+R D       C R   ++  I++  T+GF V+L+G+       +  
Sbjct: 315 NKVGGFYCKCQSGYRLD--TTTMSCKRKEFAWTTILLVTTIGFLVILLGVACIQQRMKHL 374

Query: 382 KFVKLKEEFFEKNGGLMLQQHLSQWQTSADMVRIFTQEELEKATNKYDESAVVGKGGYGT 441
           K  KL+E+FFE+NGG ML Q LS    S   V+IFT++ ++KATN Y ES ++G+GG GT
Sbjct: 375 KDTKLREQFFEQNGGGMLTQRLSGAGPSNVDVKIFTEDGMKKATNGYAESRILGQGGQGT 434

Query: 442 VYKGVLEDGLVVAIKKSKLVDQSQTSQFINEVMVLSQINHRNVVKLLGCCLETQVPLLVY 501
           VYKG+L D  +VAIKK++L D SQ  QFINEV+VLSQINHRNVVKLLGCCLET+VPLLVY
Sbjct: 435 VYKGILPDNSIVAIKKARLGDSSQVEQFINEVLVLSQINHRNVVKLLGCCLETEVPLLVY 494

Query: 502 EFITNGTLYDHIHDKANHDYSLPWEARLRIASETAGVISYLHSSASTPIIHRDIKTTNIL 561
           EFITNGTL+DH+H  +  D SL WE RL+IA E AG ++YLHSSAS PIIHRDIKT NIL
Sbjct: 495 EFITNGTLFDHLHG-SMIDSSLTWEHRLKIAIEVAGTLAYLHSSASIPIIHRDIKTANIL 554

Query: 562 LDQNYTAKVSDFGASKLVPLDQTQLSTMVQGTLGYLDPEYLLTSELTEKSDVYSFGIVLM 621
           LD N TAKV+DFGAS+L+P+D+ +L TMVQGTLGYLDPEY  T  L EKSDVYSFG+VLM
Sbjct: 555 LDVNLTAKVADFGASRLIPMDKEELETMVQGTLGYLDPEYYNTGLLNEKSDVYSFGVVLM 614

Query: 622 ELITGKKAVSFEGPEAERNLAMYVLCAMKEDRLEEVVEKGMAREGKLEQIKEVAKVAKEC 681
           EL++G+KA+ F+ P++ ++L  Y   A KE+RL+E++   +  E  L++I+E A++A EC
Sbjct: 615 ELLSGQKALCFKRPQSSKHLVSYFATATKENRLDEIIGGEVMNEDNLKEIQEAARIAAEC 674

Query: 682 LRVRGEERPSMKEVAMELEGLRV-LVENKWVNDNNLINAEEMVAYLLEDNNGASES 724
            R+ GEERP MKEVA +LE LRV   ++KW +     N   +  ++L      S S
Sbjct: 675 TRLMGEERPRMKEVAAKLEALRVEKTKHKWSDQYPEENEHLIGGHILSAQGETSSS 717

BLAST of MS015728 vs. ExPASy Swiss-Prot
Match: Q9LMN8 (Wall-associated receptor kinase 3 OS=Arabidopsis thaliana OX=3702 GN=WAK3 PE=2 SV=2)

HSP 1 Score: 587.4 bits (1513), Expect = 2.3e-166
Identity = 335/734 (45.64%), Postives = 457/734 (62.26%), Query Frame = 0

Query: 15  VVQIGILSAAAATAAAGASQALPGCEEWCGDVQIPYPFGMKEGCYL--NEKFFISCNKTH 74
           V  + I   A      G  Q    C+  CG+V I YPFG+  GCY   ++ F ++C    
Sbjct: 7   VFLVVIFFLAYTQLVKGQHQPREDCKLKCGNVTIEYPFGISTGCYYPGDDNFNLTC---- 66

Query: 75  NDSPKAFLQGGNLNVTNISISGELNILHFWARDCYPPNTVPDLSTAF---LNTEFAVSSA 134
               +  L  G + VTNIS SG +++L     +CY      +  TA    L + F++SS 
Sbjct: 67  -VVEEKLLLFGIIQVTNISHSGHVSVLFERFSECYEQKNETN-GTALGYQLGSSFSLSS- 126

Query: 135 KNKFTVIGCDTFSFISGLVGGTQFYKSACVALCDDITTVKDGACSGNGCCQLE---IP-- 194
            NKFT++GC+  S +S    G Q Y + C++LC+      +G C+G GCC  E   +P  
Sbjct: 127 NNKFTLVGCNALSLLSTF--GKQNYSTGCLSLCNS-QPEANGRCNGVGCCTTEDFSVPFD 186

Query: 195 ---------KGLNNLNYTVSSFDNHTNVLSFNPCGYAFVIEEDKFNF-SSKYIRHF-PLE 254
                    +  N +N ++  F  +T+V  FNPC YAF++E+ KFNF SSK +++   + 
Sbjct: 187 SDTFQFGSVRLRNQVNNSLDLF--NTSVYQFNPCTYAFLVEDGKFNFDSSKDLKNLRNVT 246

Query: 255 RVPLVLDWAISNNTCENT-TNCICGPSSQKVNFVEDGSEYRCQCSDGFEGNPYLPRGCQD 314
           R P+ LDW+I N TCE   +  ICG +S   N       Y C+C++G++GNPY   GC+D
Sbjct: 247 RFPVALDWSIGNQTCEQAGSTRICGKNSSCYNSTTRNG-YICKCNEGYDGNPYRSEGCKD 306

Query: 315 VDECKDGRHDCK--FECVNTEGNYTCNCPEGFRGDGRREGEGCTRNSKSFVQIIVGVTVG 374
           +DEC    H+C     C N +G + C CP G+          CTR      +I + + +G
Sbjct: 307 IDECISDTHNCSDPKTCRNRDGGFDCKCPSGY---DLNSSMSCTRPEYKRTRIFLVIIIG 366

Query: 375 FTVLLIGLTWFYLGYRKWKFVKLKEEFFEKNGGLMLQQHLSQWQTSADMVRIFTQEELEK 434
             VLL+         ++ K+ KL+ +FFE+NGG ML Q LS    S    +IFT+E +++
Sbjct: 367 VLVLLLAAICIQHATKQRKYTKLRRQFFEQNGGGMLIQRLSGAGLSNIDFKIFTEEGMKE 426

Query: 435 ATNKYDESAVVGKGGYGTVYKGVLEDGLVVAIKKSKLVDQSQTSQFINEVMVLSQINHRN 494
           ATN YDES ++G+GG GTVYKG+L D  +VAIKK++L D  Q  QFI+EV+VLSQINHRN
Sbjct: 427 ATNGYDESRILGQGGQGTVYKGILPDNTIVAIKKARLADSRQVDQFIHEVLVLSQINHRN 486

Query: 495 VVKLLGCCLETQVPLLVYEFITNGTLYDHIHDKANHDYSLPWEARLRIASETAGVISYLH 554
           VVK+LGCCLET+VPLLVYEFITNGTL+DH+H     D SL WE RLRIA E AG ++YLH
Sbjct: 487 VVKILGCCLETEVPLLVYEFITNGTLFDHLHGSI-FDSSLTWEHRLRIAIEVAGTLAYLH 546

Query: 555 SSASTPIIHRDIKTTNILLDQNYTAKVSDFGASKLVPLDQTQLSTMVQGTLGYLDPEYLL 614
           SSAS PIIHRDIKT NILLD+N TAKV+DFGASKL+P+D+ QL+TMVQGTLGYLDPEY  
Sbjct: 547 SSASIPIIHRDIKTANILLDENLTAKVADFGASKLIPMDKEQLTTMVQGTLGYLDPEYYT 606

Query: 615 TSELTEKSDVYSFGIVLMELITGKKAVSFEGPEAERNLAMYVLCAMKEDRLEEVVEKGMA 674
           T  L EKSDVYSFG+VLMEL++G+KA+ FE P+A ++L  Y + A +E+RL E+++  + 
Sbjct: 607 TGLLNEKSDVYSFGVVLMELLSGQKALCFERPQASKHLVSYFVSATEENRLHEIIDDQVL 666

Query: 675 REGKLEQIKEVAKVAKECLRVRGEERPSMKEVAMELEGLRV-LVENKWVNDNNLINAEEM 724
            E  L++I+E A++A EC R+ GEERP MKEVA +LE LRV   ++KW +     N   +
Sbjct: 667 NEDNLKEIQEAARIAAECTRLMGEERPRMKEVAAKLEALRVEKTKHKWSDQYPEENEHLI 723

BLAST of MS015728 vs. ExPASy Swiss-Prot
Match: Q9LMN6 (Wall-associated receptor kinase 4 OS=Arabidopsis thaliana OX=3702 GN=WAK4 PE=2 SV=1)

HSP 1 Score: 563.1 bits (1450), Expect = 4.6e-159
Identity = 315/690 (45.65%), Postives = 429/690 (62.17%), Query Frame = 0

Query: 34  QALPGCEEWCGDVQIPYPFGMKEGCYLNE--KFFISCNKTHNDSPKAFLQGGNLNVTNIS 93
           Q LP C E CG+V + YPFG   GC+  E   F +SC      +   F +G  L V  IS
Sbjct: 23  QTLPRCPEKCGNVTLEYPFGFSPGCWRAEDPSFNLSC-----VNENLFYKG--LEVVEIS 82

Query: 94  ISGELNILHFWARDCYPPNTVPDLSTAF-LNTEFAVSSAKNKFTVIGCDTFSFISGLVGG 153
            S +L +L+  +  CY         T +  N      S  N  T +GC++++F+S    G
Sbjct: 83  HSSQLRVLYPASYICYNSKGKFAKGTYYWSNLGNLTLSGNNTITALGCNSYAFVSS--NG 142

Query: 154 TQFYKSACVALCDDITTVKDGACSGNGCCQLEIPKGLNNLNYTVSSFDNHTNV--LSFNP 213
           T+     C++ CD ++   +G C+G GCCQ  +P G N L      FDN T+V  +S   
Sbjct: 143 TRRNSVGCISACDALSHEANGECNGEGCCQNPVPAGNNWLIVRSYRFDNDTSVQPISEGQ 202

Query: 214 CGYAFVIEEDKFNFSSKYIRHFPLER---VPLVLDWAISNNTCENTTNCICGPSSQKVNF 273
           C YAF++E  KF +++     +   R    P+VLDW+I   TC       CG +    N 
Sbjct: 203 CIYAFLVENGKFKYNASDKYSYLQNRNVGFPVVLDWSIRGETCGQVGEKKCGVNGICSNS 262

Query: 274 VEDGSEYRCQCSDGFEGNPYLPRGCQDVDECKDG----RHDCKFE--CVNTEGNYTCNCP 333
              G  Y C+C  GF+GNPYL  GCQD++EC       +H+C  +  C N  G++ CNC 
Sbjct: 263 A-SGIGYTCKCKGGFQGNPYLQNGCQDINECTTANPIHKHNCSGDSTCENKLGHFRCNC- 322

Query: 334 EGFRGDGRREGEGCTRNSK--------SFVQIIVGVTVGFTVLLIGLTWFYLGYRKWKFV 393
                  R E    T   K         +  I++G T+GF V+L+ ++      +  K  
Sbjct: 323 -----RSRYELNTTTNTCKPKGNPEYVEWTTIVLGTTIGFLVILLAISCIEHKMKNTKDT 382

Query: 394 KLKEEFFEKNGGLMLQQHLSQWQTSADMVRIFTQEELEKATNKYDESAVVGKGGYGTVYK 453
           +L+++FFE+NGG ML Q LS    S   V+IFT+E +++AT+ YDE+ ++G+GG GTVYK
Sbjct: 383 ELRQQFFEQNGGGMLMQRLSGAGPSNVDVKIFTEEGMKEATDGYDENRILGQGGQGTVYK 442

Query: 454 GVLEDGLVVAIKKSKLVDQSQTSQFINEVMVLSQINHRNVVKLLGCCLETQVPLLVYEFI 513
           G+L D  +VAIKK++L D SQ  QFINEV+VLSQINHRNVVKLLGCCLET+VPLLVYEFI
Sbjct: 443 GILPDNSIVAIKKARLGDNSQVEQFINEVLVLSQINHRNVVKLLGCCLETEVPLLVYEFI 502

Query: 514 TNGTLYDHIHDKANHDYSLPWEARLRIASETAGVISYLHSSASTPIIHRDIKTTNILLDQ 573
           ++GTL+DH+H  +  D SL WE RLR+A E AG ++YLHSSAS PIIHRDIKT NILLD+
Sbjct: 503 SSGTLFDHLHG-SMFDSSLTWEHRLRMAVEIAGTLAYLHSSASIPIIHRDIKTANILLDE 562

Query: 574 NYTAKVSDFGASKLVPLDQTQLSTMVQGTLGYLDPEYLLTSELTEKSDVYSFGIVLMELI 633
           N TAKV+DFGAS+L+P+D+  L+TMVQGTLGYLDPEY  T  L EKSDVYSFG+VLMEL+
Sbjct: 563 NLTAKVADFGASRLIPMDKEDLATMVQGTLGYLDPEYYNTGLLNEKSDVYSFGVVLMELL 622

Query: 634 TGKKAVSFEGPEAERNLAMYVLCAMKEDRLEEVVEKGMAREGKLEQIKEVAKVAKECLRV 693
           +G+KA+ FE P+  +++  Y   A KE+RL E+++  +  E    +I++ A++A EC R+
Sbjct: 623 SGQKALCFERPQTSKHIVSYFASATKENRLHEIIDGQVMNENNQREIQKAARIAVECTRL 682

Query: 694 RGEERPSMKEVAMELEGLRVL-VENKWVND 701
            GEERP MKEVA ELE LRV   ++KW ++
Sbjct: 683 TGEERPGMKEVAAELEALRVTKTKHKWSDE 695

BLAST of MS015728 vs. ExPASy TrEMBL
Match: A0A6J1CJM0 (putative wall-associated receptor kinase-like 16 OS=Momordica charantia OX=3673 GN=LOC111011910 PE=4 SV=1)

HSP 1 Score: 1502.3 bits (3888), Expect = 0.0e+00
Identity = 741/744 (99.60%), Postives = 743/744 (99.87%), Query Frame = 0

Query: 5   METLMRLIITVVQIGILSAAAATAAAGASQALPGCEEWCGDVQIPYPFGMKEGCYLNEKF 64
           METLMRLIIT VQIGILSAAAATAAAGASQALPGCEEWCGDVQIPYPFGMKEGCYLNEKF
Sbjct: 1   METLMRLIITAVQIGILSAAAATAAAGASQALPGCEEWCGDVQIPYPFGMKEGCYLNEKF 60

Query: 65  FISCNKTHNDSPKAFLQGGNLNVTNISISGELNILHFWARDCYPPNTVPDLSTAFLNTEF 124
           FISCNKTHNDSPKAFLQGGNLNVTNISISGELNILHFWARDCYPPNT+PDLSTAFLNTEF
Sbjct: 61  FISCNKTHNDSPKAFLQGGNLNVTNISISGELNILHFWARDCYPPNTLPDLSTAFLNTEF 120

Query: 125 AVSSAKNKFTVIGCDTFSFISGLVGGTQFYKSACVALCDDITTVKDGACSGNGCCQLEIP 184
           AVSSAKNKFTVIGCDTFSFISGLVGGTQFYKSACVALCDDITTVKDGACSGNGCCQLEIP
Sbjct: 121 AVSSAKNKFTVIGCDTFSFISGLVGGTQFYKSACVALCDDITTVKDGACSGNGCCQLEIP 180

Query: 185 KGLNNLNYTVSSFDNHTNVLSFNPCGYAFVIEEDKFNFSSKYIRHFPLERVPLVLDWAIS 244
           KGLNNLNYTVSSFDNHTNVLSFNPCGYAFVIEEDKFNFSSKYIRHFPLERVPLVLDWAIS
Sbjct: 181 KGLNNLNYTVSSFDNHTNVLSFNPCGYAFVIEEDKFNFSSKYIRHFPLERVPLVLDWAIS 240

Query: 245 NNTCENTTNCICGPSSQKVNFVEDGSEYRCQCSDGFEGNPYLPRGCQDVDECKDGRHDCK 304
           NNTCENTTNCICGPSS+KVNFVEDGSEYRCQCSDGFEGNPYLPRGCQDVDECKDGRHDCK
Sbjct: 241 NNTCENTTNCICGPSSRKVNFVEDGSEYRCQCSDGFEGNPYLPRGCQDVDECKDGRHDCK 300

Query: 305 FECVNTEGNYTCNCPEGFRGDGRREGEGCTRNSKSFVQIIVGVTVGFTVLLIGLTWFYLG 364
           FECVNTEGNYTCNCPEGFRGDGRREGEGCTRNSKSFVQIIVGVTVGFTVLLIGLTWFYLG
Sbjct: 301 FECVNTEGNYTCNCPEGFRGDGRREGEGCTRNSKSFVQIIVGVTVGFTVLLIGLTWFYLG 360

Query: 365 YRKWKFVKLKEEFFEKNGGLMLQQHLSQWQTSADMVRIFTQEELEKATNKYDESAVVGKG 424
           YRKWKFVKLKEEFFEKNGGLMLQQHLSQWQTSADMVRIFTQEELEKATNKYDESAVVGKG
Sbjct: 361 YRKWKFVKLKEEFFEKNGGLMLQQHLSQWQTSADMVRIFTQEELEKATNKYDESAVVGKG 420

Query: 425 GYGTVYKGVLEDGLVVAIKKSKLVDQSQTSQFINEVMVLSQINHRNVVKLLGCCLETQVP 484
           GYGTVYKGVLEDGLVVAIKKSKLVDQSQTSQFINEVMVLSQINHRNVVKLLGCCLETQVP
Sbjct: 421 GYGTVYKGVLEDGLVVAIKKSKLVDQSQTSQFINEVMVLSQINHRNVVKLLGCCLETQVP 480

Query: 485 LLVYEFITNGTLYDHIHDKANHDYSLPWEARLRIASETAGVISYLHSSASTPIIHRDIKT 544
           LLVYEFITNGTLYDHIHDKANHDYSLPWEARLRIASETAGVISYLHSSASTPIIHRDIKT
Sbjct: 481 LLVYEFITNGTLYDHIHDKANHDYSLPWEARLRIASETAGVISYLHSSASTPIIHRDIKT 540

Query: 545 TNILLDQNYTAKVSDFGASKLVPLDQTQLSTMVQGTLGYLDPEYLLTSELTEKSDVYSFG 604
           TNILLDQNYTAKVSDFGASKLVPLDQTQLSTMVQGTLGYLDPEYLLTSELTEKSDVYSFG
Sbjct: 541 TNILLDQNYTAKVSDFGASKLVPLDQTQLSTMVQGTLGYLDPEYLLTSELTEKSDVYSFG 600

Query: 605 IVLMELITGKKAVSFEGPEAERNLAMYVLCAMKEDRLEEVVEKGMAREGKLEQIKEVAKV 664
           IVLMELITGKKAVSFEGPEAERNLAMYVLCAMKEDRLEEVVEKGMAREGKLEQIKEVAKV
Sbjct: 601 IVLMELITGKKAVSFEGPEAERNLAMYVLCAMKEDRLEEVVEKGMAREGKLEQIKEVAKV 660

Query: 665 AKECLRVRGEERPSMKEVAMELEGLRVLVENKWVNDNNLINAEEMVAYLLEDNNGASEST 724
           AKECLRVRGEERPSMKEVAMELEGLRVLVENKWVNDNNLINAEEMVAYLLEDNNGASEST
Sbjct: 661 AKECLRVRGEERPSMKEVAMELEGLRVLVENKWVNDNNLINAEEMVAYLLEDNNGASEST 720

Query: 725 LLSMNNVVDDSMKVQILPRIHDGR 749
           LLSMNNVVDDSMKVQILPRIHDGR
Sbjct: 721 LLSMNNVVDDSMKVQILPRIHDGR 744

BLAST of MS015728 vs. ExPASy TrEMBL
Match: A0A6J1H843 (wall-associated receptor kinase 3-like OS=Cucurbita moschata OX=3662 GN=LOC111461403 PE=4 SV=1)

HSP 1 Score: 1121.7 bits (2900), Expect = 0.0e+00
Identity = 569/755 (75.36%), Postives = 641/755 (84.90%), Query Frame = 0

Query: 3    RPMETLMRLIITVVQIGILSAAAATAAAGASQALPGCEEWCGDVQIPYPFGMKEGCYLNE 62
            R +ETL+RL+I  V I ILS+        ASQAL GC + CGD+QIPYPFG +EGCYLN+
Sbjct: 710  RQIETLIRLMI--VNILILSSPVY-----ASQALLGCPDRCGDLQIPYPFGTREGCYLNK 769

Query: 63   KFFISCNKTHNDSPKAFLQGGNLNVTNISISGELNILHFWARDCYPPNTVPDL--STAFL 122
             F I+CN TH + P+ FL+ GN+ VTNISISGEL ILHF A+DCYP N   D   ++A L
Sbjct: 770  NFLITCNTTHFNPPRPFLRTGNIQVTNISISGELQILHFGAKDCYPKNNSLDARRTSATL 829

Query: 123  N-TEFAVSSAKNKFTVIGCDTFSFISGLVGGTQFYKSACVALCDDITTVKDGACSGNGCC 182
            N + F VSS KNKFTVIGCDT++F+SG + G Q Y++ACVALCD+ITTV+DGACSGNGCC
Sbjct: 830  NLSTFTVSSTKNKFTVIGCDTYAFLSGQIEG-QSYRTACVALCDNITTVRDGACSGNGCC 889

Query: 183  QLEIPKGLNNLNYTVSSFDNHTNVLSFNPCGYAFVIEEDKFNFSSKYIRHFPLERVPLVL 242
            QL+IP GL  L Y V SFDNHT+VLSFNPCGYAFV EEDKF+FS+ YIR FP  +VP+VL
Sbjct: 890  QLDIPSGLKGLRYRVRSFDNHTDVLSFNPCGYAFVTEEDKFHFSAAYIRDFPQRQVPVVL 949

Query: 243  DWAISNNTC---ENTTNCICGPSSQKVNFVEDGSEYRCQCSDGFEGNPYLPRGCQDVDEC 302
            DW ISN TC    N +NCICGP+S  VN + DGSEYRC+C DGFEGNPYLPRGCQD+DEC
Sbjct: 950  DWGISNTTCSTANNKSNCICGPNSMMVNPLHDGSEYRCRCLDGFEGNPYLPRGCQDIDEC 1009

Query: 303  KDGR-HDCKFECVNTEGNYTCNCPEGFRGDGRREGEGCTRNSKSFVQIIVGVTVGFTVLL 362
            +D R +DCKFECVNT+GNYTCNCPEGF+GDGRR GEGCTR+SKSFVQ+I+GV+VGFTVL+
Sbjct: 1010 RDERLNDCKFECVNTKGNYTCNCPEGFKGDGRRGGEGCTRSSKSFVQVIIGVSVGFTVLV 1069

Query: 363  IGLTWFYLGYRKWKFVKLKEEFFEKNGGLMLQQHLSQWQTSADMVRIFTQEELEKATNKY 422
            IG TW YLGYRKWK +KLKE+FFE+NGGLMLQ+HLSQW++S D V IFTQEEL+KATNKY
Sbjct: 1070 IGSTWLYLGYRKWKLIKLKEKFFEENGGLMLQRHLSQWKSSTDTVTIFTQEELDKATNKY 1129

Query: 423  DESAVVGKGGYGTVYKGVLEDGLVVAIKKSKLVDQSQTSQFINEVMVLSQINHRNVVKLL 482
            DESAV+GKGGYGTVYKG L DG VVAIKKSKLVDQSQTSQFINEV+VLSQINHRNVVKLL
Sbjct: 1130 DESAVIGKGGYGTVYKGFLPDGSVVAIKKSKLVDQSQTSQFINEVIVLSQINHRNVVKLL 1189

Query: 483  GCCLETQVPLLVYEFITNGTLYDHIHDKANHDYSLPWEARLRIASETAGVISYLHSSAST 542
            GCCLETQVPLLVYEF+TNGTL+DHIHD   H   L WEARLRIASETAGVISYLHSSAST
Sbjct: 1190 GCCLETQVPLLVYEFVTNGTLFDHIHDTTKH-VPLSWEARLRIASETAGVISYLHSSAST 1249

Query: 543  PIIHRDIKTTNILLDQNYTAKVSDFGASKLVPLDQTQLSTMVQGTLGYLDPEYLLTSELT 602
            PIIHRDIKTTNILLD NY AKVSDFGASKLVPLDQTQLSTMVQGTLGYLDPEYLLTSELT
Sbjct: 1250 PIIHRDIKTTNILLDDNYNAKVSDFGASKLVPLDQTQLSTMVQGTLGYLDPEYLLTSELT 1309

Query: 603  EKSDVYSFGIVLMELITGKKAVSFEGPEAERNLAMYVLCAMKEDRLEEVVEKG-MAREGK 662
            EKSDVYSFGIVL+ELITGKKAVSFEGPEAERNLAMYVLCAMKEDRL +VVEKG MARE +
Sbjct: 1310 EKSDVYSFGIVLLELITGKKAVSFEGPEAERNLAMYVLCAMKEDRLGDVVEKGIMARERE 1369

Query: 663  LEQIKEVAKVAKECLRVRGEERPSMKEVAMELEGLRVLVENKWVNDNNLINAEEMVAYLL 722
             EQIKEV KVA++CLR+ GEERPSMKEVAMELEGLRV+VE++WVN+ N++          
Sbjct: 1370 FEQIKEVGKVARKCLRISGEERPSMKEVAMELEGLRVMVEHQWVNEENMV---------- 1429

Query: 723  EDNNGASESTLLS-MNNVVDDSMKVQILPRIHDGR 749
               +GAS   ++S   NVVDDSMKVQ+LP IHDGR
Sbjct: 1430 --ADGASHMFVVSGSTNVVDDSMKVQVLPLIHDGR 1443

BLAST of MS015728 vs. ExPASy TrEMBL
Match: A0A6J1JNA5 (wall-associated receptor kinase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111486858 PE=4 SV=1)

HSP 1 Score: 1120.9 bits (2898), Expect = 0.0e+00
Identity = 564/754 (74.80%), Postives = 640/754 (84.88%), Query Frame = 0

Query: 3   RPMETLMRLIITVVQIGILSAAAATAAAGASQALPGCEEWCGDVQIPYPFGMKEGCYLNE 62
           R ++T +RL+I  + I ILS    +  A ASQAL GC++ CGD+QIPYPFG +EGCYLN+
Sbjct: 3   RQIKTHIRLMI--INILILS---PSVYAVASQALLGCDDRCGDLQIPYPFGTREGCYLNK 62

Query: 63  KFFISCNKTHNDSPKAFLQGGNLNVTNISISGELNILHFWARDCYPPNTVPDL--STAFL 122
            F I+CN TH D P+ FL+ GN+ VTNISISGEL ILHF A+DCYP N   D   ++A L
Sbjct: 63  NFLITCNTTHFDPPRPFLRTGNIQVTNISISGELQILHFGAKDCYPKNNSLDARRTSATL 122

Query: 123 N-TEFAVSSAKNKFTVIGCDTFSFISGLVGGTQFYKSACVALCDDITTVKDGACSGNGCC 182
           N + F VSS KNKFTVIGCDT++F+SG + G Q Y++ACVALCD+ITTV+DGACSGNGCC
Sbjct: 123 NLSTFTVSSTKNKFTVIGCDTYAFLSGQIEG-QSYRTACVALCDNITTVRDGACSGNGCC 182

Query: 183 QLEIPKGLNNLNYTVSSFDNHTNVLSFNPCGYAFVIEEDKFNFSSKYIRHFPLERVPLVL 242
           QL+IP GL  L Y V SFDNHT+V SFNPCGYAFV EEDKF+FS+ YIR+FP  +VP+VL
Sbjct: 183 QLDIPSGLKGLRYRVRSFDNHTDVFSFNPCGYAFVTEEDKFHFSAAYIRNFPQRQVPVVL 242

Query: 243 DWAISNNTC---ENTTNCICGPSSQKVNFVEDGSEYRCQCSDGFEGNPYLPRGCQDVDEC 302
           DW ISN TC    N +NCICGP+S  VN + DGSEYRC C DGFEGNPYLPRGCQD+DEC
Sbjct: 243 DWGISNTTCSTANNKSNCICGPNSMMVNVLPDGSEYRCGCLDGFEGNPYLPRGCQDIDEC 302

Query: 303 KDGR-HDCKFECVNTEGNYTCNCPEGFRGDGRREGEGCTRNSKSFVQIIVGVTVGFTVLL 362
           +D R +DCKFECVNT+GNYTCNCPEGF+GDGRR GEGCTR+SKSFVQ+I+GV+VGFTVL+
Sbjct: 303 RDERLNDCKFECVNTKGNYTCNCPEGFKGDGRRGGEGCTRSSKSFVQVIIGVSVGFTVLV 362

Query: 363 IGLTWFYLGYRKWKFVKLKEEFFEKNGGLMLQQHLSQWQTSADMVRIFTQEELEKATNKY 422
           IG TW YLGYRKWK +KLKE+FFE+NGGLMLQ+HLSQW++S D V IFTQEEL+KATNKY
Sbjct: 363 IGSTWLYLGYRKWKLIKLKEKFFEENGGLMLQRHLSQWKSSTDTVTIFTQEELDKATNKY 422

Query: 423 DESAVVGKGGYGTVYKGVLEDGLVVAIKKSKLVDQSQTSQFINEVMVLSQINHRNVVKLL 482
           DESAV+GKGGYGTVYKG+L DG VVAIKKSKLVDQSQTSQFINEV+VLSQINHRNVVKLL
Sbjct: 423 DESAVIGKGGYGTVYKGILPDGSVVAIKKSKLVDQSQTSQFINEVIVLSQINHRNVVKLL 482

Query: 483 GCCLETQVPLLVYEFITNGTLYDHIHDKANHDYSLPWEARLRIASETAGVISYLHSSAST 542
           GCCLETQVPLLVYEF+TNGTL+DHIHD   H   L W+ARLRIA ETAGVISYLHSSAST
Sbjct: 483 GCCLETQVPLLVYEFVTNGTLFDHIHDTTKH-VPLSWKARLRIALETAGVISYLHSSAST 542

Query: 543 PIIHRDIKTTNILLDQNYTAKVSDFGASKLVPLDQTQLSTMVQGTLGYLDPEYLLTSELT 602
           PIIHRDIKTTNILLD NY AKVSDFGASKLVPLDQTQLSTMVQGTLGYLDPEYLLTSELT
Sbjct: 543 PIIHRDIKTTNILLDDNYNAKVSDFGASKLVPLDQTQLSTMVQGTLGYLDPEYLLTSELT 602

Query: 603 EKSDVYSFGIVLMELITGKKAVSFEGPEAERNLAMYVLCAMKEDRLEEVVEKGMAREGKL 662
           EKSDVYSFGIVL+ELITGKKAVSFEGPEAERNLAMYVLCAMKEDRL EVVEKGMAREG+ 
Sbjct: 603 EKSDVYSFGIVLLELITGKKAVSFEGPEAERNLAMYVLCAMKEDRLGEVVEKGMAREGEF 662

Query: 663 EQIKEVAKVAKECLRVRGEERPSMKEVAMELEGLRVLVENKWVNDNNLINAEEMVAYLLE 722
           EQIK+V KVA++CLR+ GEERPSMKEV MELEGLRV+ E++WV++ NL+           
Sbjct: 663 EQIKDVGKVARKCLRISGEERPSMKEVVMELEGLRVMAEHEWVDEENLV----------- 722

Query: 723 DNNGASESTLLS-MNNVVDDSMKVQILPRIHDGR 749
             +GAS   ++S   NVVDDSMKVQ+LP IHDGR
Sbjct: 723 -ADGASNIFVVSGSTNVVDDSMKVQVLPLIHDGR 737

BLAST of MS015728 vs. ExPASy TrEMBL
Match: A0A6J1CJ33 (putative wall-associated receptor kinase-like 16 OS=Momordica charantia OX=3673 GN=LOC111011918 PE=4 SV=1)

HSP 1 Score: 1044.3 bits (2699), Expect = 2.5e-301
Identity = 543/745 (72.89%), Postives = 615/745 (82.55%), Query Frame = 0

Query: 16  VQIGILSAAAATAAAGASQALPGCEEWCGDVQIPYPFGMKEGCYLNEKFFISC---NKTH 75
           V I ILS         ASQALPGC+EWCGD++IPYPFGMKE CYLN+ F I+C   N  +
Sbjct: 3   VNIAILSTPII-----ASQALPGCDEWCGDLRIPYPFGMKEECYLNKTFSINCKKPNNNN 62

Query: 76  NDSPKAFLQGGNLNVTNISISGELNILHFWARDCY------PPNTVPDLSTAFLNTEFAV 135
           N+ P AFL   N++VT I+ISGEL+++    RDCY        ++VP++++      F V
Sbjct: 63  NEPPTAFLMDTNISVTKIAISGELHVMQPIVRDCYNVPTHHNDSSVPNITSLSAPAIFPV 122

Query: 136 SSAKNKFTVIGCDTFSFISGLVGGTQFYKSACVALCDDITTVKDGACSGNGCCQLEIPKG 195
           ++AKNKF  +GC+T   I G + G++ Y S CV++C + + + +G+CSG+GCCQLEIPKG
Sbjct: 123 AAAKNKFVTVGCNTLGLIGGELNGSE-YVSGCVSVCLNESAIVNGSCSGSGCCQLEIPKG 182

Query: 196 LNNLNYTVSSFD-NHTNVLSFNPCGYAFVIEEDKFNFSSKYIRHFPLERVPLVLDWAISN 255
           L NL+  + S   N+T  L FNPCGYAFV E++ F FSSKYI +F  E V +VLDW I+N
Sbjct: 183 LRNLSLAMYSIPLNYT--LDFNPCGYAFVTEDEGFEFSSKYITNFGDEEVGVVLDWGITN 242

Query: 256 NTCENTTNCICGP-SSQKVNFVEDGSEYRCQCSDGFEGNPYLPRGCQDVDECKDGRHDCK 315
            T  +     CG  S++  +F +DGS+YRC+C  GF+GNPYLP+GCQD+DECK+  HDCK
Sbjct: 243 QTNYHQ----CGQNSTRNSSFSDDGSQYRCECFKGFDGNPYLPQGCQDIDECKNDTHDCK 302

Query: 316 FECVNTEGNYTCNCPEGFRGDGRREGEGCTRNSKSFVQIIVGVTVGFTVLLIGLTWFYLG 375
           +ECVNTEGNYTCNCP+ F+GDGRR GEGCT NSKS++ II+GV VGFTVLLI  + FYLG
Sbjct: 303 YECVNTEGNYTCNCPKDFKGDGRRGGEGCTPNSKSYIHIILGVGVGFTVLLIVSSRFYLG 362

Query: 376 YRKWKFVKLKEEFFEKNGGLMLQQHLSQWQTSADMVRIFTQEELEKATNKYDESAVVGKG 435
           YRKWKF+KLKEEFFEKNGGLMLQ HLSQWQ+SADMVRIFTQEELEKATNKYDESAVVGKG
Sbjct: 363 YRKWKFLKLKEEFFEKNGGLMLQHHLSQWQSSADMVRIFTQEELEKATNKYDESAVVGKG 422

Query: 436 GYGTVYKGVLEDGLVVAIKKSKLVDQSQTSQFINEVMVLSQINHRNVVKLLGCCLETQVP 495
           GYGTVYKGVLEDGLVVAIKKSKLVDQSQTSQFINEVMVLSQINHRNVVKLLGCCLETQVP
Sbjct: 423 GYGTVYKGVLEDGLVVAIKKSKLVDQSQTSQFINEVMVLSQINHRNVVKLLGCCLETQVP 482

Query: 496 LLVYEFITNGTLYDHIHDKANHDYSLPWEARLRIASETAGVISYLHSSASTPIIHRDIKT 555
           LLVYEFITNGTLYDHIHDK NH+ SLPWEARLRIASETAGVISYLHSSASTPIIHRDIKT
Sbjct: 483 LLVYEFITNGTLYDHIHDKDNHN-SLPWEARLRIASETAGVISYLHSSASTPIIHRDIKT 542

Query: 556 TNILLDQNYTAKVSDFGASKLVPLDQTQLSTMVQGTLGYLDPEYLLTSELTEKSDVYSFG 615
           TNILLDQNYTAKVSDFGASKLVPLDQTQLSTMVQGTLGYLDPEYLLTSELTEKSDVYSFG
Sbjct: 543 TNILLDQNYTAKVSDFGASKLVPLDQTQLSTMVQGTLGYLDPEYLLTSELTEKSDVYSFG 602

Query: 616 IVLMELITGKKAVSFEGPEAERNLAMYVLCAMKEDRLEEVVEKGMAREGKLEQIKEVAKV 675
           IVLMELITGKKAVSF+GPEAERNLAMYVL AMKEDRLEEVVEK MAREG LEQIKEVAKV
Sbjct: 603 IVLMELITGKKAVSFKGPEAERNLAMYVLSAMKEDRLEEVVEKEMAREGTLEQIKEVAKV 662

Query: 676 AKECLRVRGEERPSMKEVAMELEGLRVL-VENKWVNDNNLINAEEMVAYLLEDNNGASES 735
           AKECLRVRGEERPSMKEVAMELEGLRVL VE+ WVND N  NAEEM A LL+ +   S+ 
Sbjct: 663 AKECLRVRGEERPSMKEVAMELEGLRVLQVEHSWVNDKNSSNAEEM-ACLLDGDIELSQY 722

Query: 736 TLLSMNNVVDDSMKVQILPRIHDGR 749
            +    N V DS+K QI+ +IHDGR
Sbjct: 723 VVSDSINTVGDSIKAQIVSQIHDGR 733

BLAST of MS015728 vs. ExPASy TrEMBL
Match: A0A1S3B3B1 (wall-associated receptor kinase 2-like OS=Cucumis melo OX=3656 GN=LOC103485676 PE=4 SV=1)

HSP 1 Score: 1044.3 bits (2699), Expect = 2.5e-301
Identity = 537/769 (69.83%), Postives = 630/769 (81.92%), Query Frame = 0

Query: 3   RPMETLMRL----IITVVQIGILSAAAATAA--AGASQALPGCEEWCGDVQIPYPFGMKE 62
           RP ETL+RL    II ++    + A+A T A    ASQALPGC+EWCGDV+IPYPFGM+E
Sbjct: 3   RPTETLIRLTAVKIIVILSAAFVVASATTNATVTVASQALPGCDEWCGDVRIPYPFGMRE 62

Query: 63  GCYLNEKFFISCNKTHNDSPKAFLQ--GGNLNVTNIS-ISGELNILHFWARDCYPPNTV- 122
           GCYLN + F+    +   SP + +   G N+ +TNIS I+GE+ +L+  A+ C+PP+T  
Sbjct: 63  GCYLNNETFLLQCLSPAGSPNSLMPVLGPNI-ITNISIITGEIKLLNRVAQACHPPDTAT 122

Query: 123 ---PDLSTAFLNTEFAVSSAKNKFTVIGCDTFSFISGLVGGTQFYKSACVALCDDITTVK 182
              PD+     N  + +S  KNKF VIGCDTF+ +SG V   Q ++S C+ALCD+I TVK
Sbjct: 123 DVFPDMVVGIPN--YTISHTKNKFVVIGCDTFAIVSGNVVDGQSFQSGCLALCDNIGTVK 182

Query: 183 DGACSGNGCCQLEIPKGLNNLNYTVSSFDNHTNVLSFNPCGYAFVIEEDKFNFSSKYIRH 242
           DGACSG+GCCQL++P GL+++ + V+S  NHTNV SFNPCGYAFV E D F+FSSKYIR+
Sbjct: 183 DGACSGSGCCQLDLPSGLDSMVFEVASLTNHTNVSSFNPCGYAFVTERDSFDFSSKYIRN 242

Query: 243 FPLERVPLVLDWAISNNTC---ENTTNCICGPSSQKVNFVEDGSEYRCQCSDGFEGNPYL 302
           FP + VP+VLDWAISN+TC   +N TNC+CG +S KV+ ++D S YRCQC DGFEGNPYL
Sbjct: 243 FPEKIVPVVLDWAISNDTCVTAKNKTNCVCGKNSTKVDLLDDPSRYRCQCLDGFEGNPYL 302

Query: 303 PRGCQDVDECKDGR-HDCKFECVNTEGNYTCNCPEGFRGDGRREGEGCTRNSKSFVQIIV 362
           P GCQD+DECKD   +DC+FECVNT G+YTCNCPE F+GDG+R+G+GCTRNSKSFVQIIV
Sbjct: 303 PDGCQDIDECKDDNLNDCRFECVNTIGSYTCNCPEDFKGDGKRQGDGCTRNSKSFVQIIV 362

Query: 363 GVTVGFTVLLIGLTWFYLGYRKWKFVKLKEEFFEKNGGLMLQQHLSQWQTSAD-MVRIFT 422
           GVTVGFTVL+IG  W YLGY+KWKF+KLKE+FFEKNGGLMLQQHLSQWQ S D MVRIFT
Sbjct: 363 GVTVGFTVLVIGSAWLYLGYKKWKFLKLKEKFFEKNGGLMLQQHLSQWQASTDHMVRIFT 422

Query: 423 QEELEKATNKYDESAVVGKGGYGTVYKGVLEDGLVVAIKKSKLVDQSQTSQFINEVMVLS 482
           +E+L+KATNKYD+SAVVGKGG+GTVYKGVL+DG VVAIKKSKLVDQSQT QFINEV+VLS
Sbjct: 423 KEDLDKATNKYDDSAVVGKGGFGTVYKGVLDDGSVVAIKKSKLVDQSQTDQFINEVIVLS 482

Query: 483 QINHRNVVKLLGCCLETQVPLLVYEFITNGTLYDHIHDKANHDYSLPWEARLRIASETAG 542
           QINHRNVVKLLGCCLET+VPLLVYEFI+NGTLY+++HDK N   SL WEARLRIA+ETAG
Sbjct: 483 QINHRNVVKLLGCCLETEVPLLVYEFISNGTLYEYVHDKTNDRNSLSWEARLRIAAETAG 542

Query: 543 VISYLHSSASTPIIHRDIKTTNILLDQNYTAKVSDFGASKLVPLDQTQLSTMVQGTLGYL 602
           VISYLHSSASTPIIHRDIKTTNILLD NYTAKVSDFGASKLVP+DQTQLSTMVQGTLGYL
Sbjct: 543 VISYLHSSASTPIIHRDIKTTNILLDHNYTAKVSDFGASKLVPMDQTQLSTMVQGTLGYL 602

Query: 603 DPEYLLTSELTEKSDVYSFGIVLMELITGKKAVSFEGPEAERNLAMYVLCAMKEDRLEEV 662
           DPEYLLTSELTEKSDVYSFGIVL+ELITGKKAVSFEGPEAERNLAMYV+CAMKE+RLEEV
Sbjct: 603 DPEYLLTSELTEKSDVYSFGIVLLELITGKKAVSFEGPEAERNLAMYVMCAMKENRLEEV 662

Query: 663 VEKGMAREGKLEQIKEVAKVAKECLRVRGEERPSMKEVAMELEGLRVLVENKWVNDNNLI 722
           VEKGMA +  +EQIKE AK+A  CLR++GEERPSMKEVA +LEGLR L E    N+    
Sbjct: 663 VEKGMATKANIEQIKEAAKLATTCLRIKGEERPSMKEVATDLEGLRGLNE---ANEKLES 722

Query: 723 NAEEMVAYLLEDNNGASESTLLSMN-----NVVDDSMKVQILPRIHDGR 749
             E MV   ++ ++GA EST    +     N+VDDSMKV ILP IH+GR
Sbjct: 723 KGETMVGIWVQ-SDGALESTSKQFSASGSTNIVDDSMKVHILPLIHNGR 764

BLAST of MS015728 vs. TAIR 10
Match: AT1G21270.1 (wall-associated kinase 2 )

HSP 1 Score: 617.1 bits (1590), Expect = 1.9e-176
Identity = 326/675 (48.30%), Postives = 451/675 (66.81%), Query Frame = 0

Query: 39  CEEWCGDVQIPYPFGMKEGCYL--NEKFFISCNKTHNDSPKAFLQGGNLNVTNISISGEL 98
           C+  CG+V + YPFG   GCY   +E F ++C    N+  K F   GN+ V N+S+SG+L
Sbjct: 29  CQTRCGNVAVEYPFGTSPGCYYPGDESFNLTC----NEQEKLFF--GNMPVINMSLSGQL 88

Query: 99  NILHFWARDCYPPNTVPDLSTAFLNT--EFAVSSAKNKFTVIGCDTFSFISGLVGGTQFY 158
            +    +R CY          A   T   F +S   N+FTV+GC++++F+     G + Y
Sbjct: 89  RVRLVRSRVCYDSQGKQTDYIAQRTTLGNFTLSEL-NRFTVVGCNSYAFLR--TSGVEKY 148

Query: 159 KSACVALCDDITTVKDGACSGNGCCQLEIPKGLNNLNYTVSSFDNHTNVLSFNPCGYAFV 218
            + C+++CD  TT K+G+CSG GCCQ+ +P+G + +     SF NH  V  FNPC YAF+
Sbjct: 149 STGCISICDSATT-KNGSCSGEGCCQIPVPRGYSFVRVKPHSFHNHPTVHLFNPCTYAFL 208

Query: 219 IEEDKFNFSS----KYIRHFPLERVPLVLDWAISNNTCENTT-NCICGPSSQKVNFVEDG 278
           +E+  F+F +      +R+  +   P+VLDW+I + TC+      +CG +S   +    G
Sbjct: 209 VEDGMFDFHALEDLNNLRN--VTTFPVVLDWSIGDKTCKQVEYRGVCGGNSTCFDST-GG 268

Query: 279 SEYRCQCSDGFEGNPYLPRGCQDVDECKDGRHDCK--FECVNTEGNYTCNCPEGFRGDGR 338
           + Y C+C +GFEGNPYLP GCQD++EC   RH+C     C NT+G++ CNCP G+R D  
Sbjct: 269 TGYNCKCLEGFEGNPYLPNGCQDINECISSRHNCSEHSTCENTKGSFNCNCPSGYRKDSL 328

Query: 339 REGEGCTRNSK----SFVQIIVGVTVGFTVLLIGLTWFYLGYRKWKFVKLKEEFFEKNGG 398
                CTR  +     + QI +G T+GF+V+++G++      +  K  +L+++FFE+NGG
Sbjct: 329 ---NSCTRKVRPEYFRWTQIFLGTTIGFSVIMLGISCLQQKIKHRKNTELRQKFFEQNGG 388

Query: 399 LMLQQHLSQWQTSADMVRIFTQEELEKATNKYDESAVVGKGGYGTVYKGVLEDGLVVAIK 458
            ML Q +S    S   V+IFT++ +++ATN Y ES ++G+GG GTVYKG+L D  +VAIK
Sbjct: 389 GMLIQRVSGAGPSNVDVKIFTEKGMKEATNGYHESRILGQGGQGTVYKGILPDNSIVAIK 448

Query: 459 KSKLVDQSQTSQFINEVMVLSQINHRNVVKLLGCCLETQVPLLVYEFITNGTLYDHIHDK 518
           K++L ++SQ  QFINEV+VLSQINHRNVVK+LGCCLET+VPLLVYEFI +GTL+DH+H  
Sbjct: 449 KARLGNRSQVEQFINEVLVLSQINHRNVVKVLGCCLETEVPLLVYEFINSGTLFDHLHGS 508

Query: 519 ANHDYSLPWEARLRIASETAGVISYLHSSASTPIIHRDIKTTNILLDQNYTAKVSDFGAS 578
             +D SL WE RLRIA+E AG ++YLHSSAS PIIHRDIKT NILLD+N TAKV+DFGAS
Sbjct: 509 L-YDSSLTWEHRLRIATEVAGSLAYLHSSASIPIIHRDIKTANILLDKNLTAKVADFGAS 568

Query: 579 KLVPLDQTQLSTMVQGTLGYLDPEYLLTSELTEKSDVYSFGIVLMELITGKKAVSFEGPE 638
           +L+P+D+ QL+T+VQGTLGYLDPEY  T  L EKSDVYSFG+VLMEL++G+KA+ FE P 
Sbjct: 569 RLIPMDKEQLTTIVQGTLGYLDPEYYNTGLLNEKSDVYSFGVVLMELLSGQKALCFERPH 628

Query: 639 AERNLAMYVLCAMKEDRLEEVVEKGMAREGKLEQIKEVAKVAKECLRVRGEERPSMKEVA 698
             +NL      A K +R  E+++  +  E    +I+E A++A EC R+ GEERP MKEVA
Sbjct: 629 CPKNLVSCFASATKNNRFHEIIDGQVMNEDNQREIQEAARIAAECTRLMGEERPRMKEVA 686

BLAST of MS015728 vs. TAIR 10
Match: AT1G21230.1 (wall associated kinase 5 )

HSP 1 Score: 599.4 bits (1544), Expect = 4.1e-171
Identity = 330/708 (46.61%), Postives = 450/708 (63.56%), Query Frame = 0

Query: 33  SQALPGCEEWCGDVQIPYPFGMKEGCYL--NEKFFISCNKTHNDSPKAFLQGGNLNVTNI 92
           +Q    C+  CGDV I YPFG+  GCY   ++ F I+C +   D P       N+ V N 
Sbjct: 23  AQPRDDCQTRCGDVPIDYPFGISTGCYYPGDDSFNITCEE---DKPNVL---SNIEVLNF 82

Query: 93  SISGELNILHFWARDCYPPNTVPDLSTAFLNTEFAVSSAKNKFTVIGCDTFSFISGLVGG 152
           + SG+L  L   +  CY   T  D  + +   +    S  NKFT++GC+ ++ +S    G
Sbjct: 83  NHSGQLRGLIPRSTVCYDQQTNNDFESLWFRLDNLSFSPNNKFTLVGCNAWALLSTF--G 142

Query: 153 TQFYKSACVALCDDITTVKDGACSGNGCCQLE--IPKGLNNLNYTVSSFDNHTNVLSFNP 212
            Q Y + C++LC D     +  C+G GCC+ E  IP   + +    S F+N T+V  FNP
Sbjct: 143 IQNYSTGCMSLC-DTPPPPNSKCNGVGCCRTEVSIPLDSHRIETQPSRFENMTSVEHFNP 202

Query: 213 CGYAFVIEEDKFNFSS----KYIRHFPLERVPLVLDWAISNNTCENTT-NCICGPSSQKV 272
           C YAF +E+  FNFSS    K +R+  + R P++LDW+I N TCE      ICG +S   
Sbjct: 203 CSYAFFVEDGMFNFSSLEDLKDLRN--VTRFPVLLDWSIGNQTCEQVVGRNICGGNSTCF 262

Query: 273 NFVEDGSEYRCQCSDGFEGNPYLPRGCQDVDECKDGRHDCK--FECVNTEGNYTCNCPEG 332
           +    G  Y C+C  GF+GNPYL  GCQD++EC    H+C     C NT G++ C CP G
Sbjct: 263 DSTR-GKGYNCKCLQGFDGNPYLSDGCQDINECTTRIHNCSDTSTCENTLGSFHCQCPSG 322

Query: 333 FRGDGRREGEGCTRNSK------SFVQIIVGVTVGFTVLLIGLTWFYLGYRKWKFVKLKE 392
              D       C    K       +  +++G T+GF ++L+ +++     R  K  +L++
Sbjct: 323 --SDLNTTTMSCIDTPKEEPKYLGWTTVLLGTTIGFLIILLTISYIQQKMRHRKNTELRQ 382

Query: 393 EFFEKNGGLMLQQHLSQWQTSADMVRIFTQEELEKATNKYDESAVVGKGGYGTVYKGVLE 452
           +FFE+NGG ML Q LS    S   V+IFT+E +++AT+ Y+ES ++G+GG GTVYKG+L+
Sbjct: 383 QFFEQNGGGMLIQRLSGAGPSNVDVKIFTEEGMKEATDGYNESRILGQGGQGTVYKGILQ 442

Query: 453 DGLVVAIKKSKLVDQSQTSQFINEVMVLSQINHRNVVKLLGCCLETQVPLLVYEFITNGT 512
           D  +VAIKK++L D+SQ  QFINEV+VLSQINHRNVVKLLGCCLET+VPLLVYEFI++GT
Sbjct: 443 DNSIVAIKKARLGDRSQVEQFINEVLVLSQINHRNVVKLLGCCLETEVPLLVYEFISSGT 502

Query: 513 LYDHIHDKANHDYSLPWEARLRIASETAGVISYLHSSASTPIIHRDIKTTNILLDQNYTA 572
           L+DH+H  +  D SL WE RLRIA E AG ++YLHS AS PIIHRD+KT NILLD+N TA
Sbjct: 503 LFDHLHG-SMFDSSLTWEHRLRIAIEVAGTLAYLHSYASIPIIHRDVKTANILLDENLTA 562

Query: 573 KVSDFGASKLVPLDQTQLSTMVQGTLGYLDPEYLLTSELTEKSDVYSFGIVLMELITGKK 632
           KV+DFGAS+L+P+DQ QL+TMVQGTLGYLDPEY  T  L EKSDVYSFG+VLMEL++G+K
Sbjct: 563 KVADFGASRLIPMDQEQLTTMVQGTLGYLDPEYYNTGLLNEKSDVYSFGVVLMELLSGEK 622

Query: 633 AVSFEGPEAERNLAMYVLCAMKEDRLEEVVEKGMAREGKLEQIKEVAKVAKECLRVRGEE 692
           A+ FE P++ ++L  Y + AMKE+RL E+++  +  E    +I+E A++A EC R+ GEE
Sbjct: 623 ALCFERPQSSKHLVSYFVSAMKENRLHEIIDGQVMNEYNQREIQESARIAVECTRIMGEE 682

Query: 693 RPSMKEVAMELEGLRVLVENKWVNDNNLINAEEMVAYLLEDNNGASES 724
           RPSMKEVA ELE LRV       +D      E ++   +    G + S
Sbjct: 683 RPSMKEVAAELEALRVKTTKHQWSDQYPKEVEHLLGVQILSTQGDTSS 715

BLAST of MS015728 vs. TAIR 10
Match: AT1G21250.1 (cell wall-associated kinase )

HSP 1 Score: 597.0 bits (1538), Expect = 2.0e-170
Identity = 331/716 (46.23%), Postives = 449/716 (62.71%), Query Frame = 0

Query: 22  SAAAATAAAGASQALPGCEEWCGDVQIPYPFGMKEGCYL--NEKFFISCNKTHNDSPKAF 81
           S A      G  Q    C+  CG++ I YPFG+  GCY   NE F I+C +   D P   
Sbjct: 15  SLACTQLVKGQHQPGENCQNKCGNITIEYPFGISSGCYYPGNESFSITCKE---DRPHVL 74

Query: 82  LQGGNLNVTNISISGELNILHFWARDCYPPNTVPDLSTAFLNTEFAVSSAKNKFTVIGCD 141
               ++ V N + SG+L +L   +  CY          +    E    SA NK T +GC+
Sbjct: 75  ---SDIEVANFNHSGQLQVLLNRSSTCYDEQGKKTEEDSSFTLENLSLSANNKLTAVGCN 134

Query: 142 TFSFISGLVGGTQFYKSACVALCDDITTVKDGACSGNGCCQLEIPKGLNNLNYTVSS--F 201
             S +     G Q Y +AC++LCD      DG C+G GCC++++   L++  +  +S   
Sbjct: 135 ALSLLDTF--GMQNYSTACLSLCDS-PPEADGECNGRGCCRVDVSAPLDSYTFETTSGRI 194

Query: 202 DNHTNVLSFNPCGYAFVIEEDKFNFSS--KYIRHFPLERVPLVLDWAISNNTCENT-TNC 261
            + T+   F+PC YAF++E+DKFNFSS    +    + R P++LDW++ N TCE   +  
Sbjct: 195 KHMTSFHDFSPCTYAFLVEDDKFNFSSTEDLLNLRNVMRFPVLLDWSVGNQTCEQVGSTS 254

Query: 262 ICGPSSQKVNFVEDGSEYRCQCSDGFEGNPYLPRGCQDVDECKDG----RHDCK--FECV 321
           ICG +S  ++       Y C+C++GF+GNPYL  GCQDV+EC       RH+C     C 
Sbjct: 255 ICGGNSTCLDSTPRNG-YICRCNEGFDGNPYLSAGCQDVNECTTSSTIHRHNCSDPKTCR 314

Query: 322 NTEGNYTCNCPEGFRGDGRREGEGCTRNSKSFVQIIVGVTVGFTVLLIGLTWFYLGYRKW 381
           N  G + C C  G+R D       C R   ++  I++  T+GF V+L+G+       +  
Sbjct: 315 NKVGGFYCKCQSGYRLD--TTTMSCKRKEFAWTTILLVTTIGFLVILLGVACIQQRMKHL 374

Query: 382 KFVKLKEEFFEKNGGLMLQQHLSQWQTSADMVRIFTQEELEKATNKYDESAVVGKGGYGT 441
           K  KL+E+FFE+NGG ML Q LS    S   V+IFT++ ++KATN Y ES ++G+GG GT
Sbjct: 375 KDTKLREQFFEQNGGGMLTQRLSGAGPSNVDVKIFTEDGMKKATNGYAESRILGQGGQGT 434

Query: 442 VYKGVLEDGLVVAIKKSKLVDQSQTSQFINEVMVLSQINHRNVVKLLGCCLETQVPLLVY 501
           VYKG+L D  +VAIKK++L D SQ  QFINEV+VLSQINHRNVVKLLGCCLET+VPLLVY
Sbjct: 435 VYKGILPDNSIVAIKKARLGDSSQVEQFINEVLVLSQINHRNVVKLLGCCLETEVPLLVY 494

Query: 502 EFITNGTLYDHIHDKANHDYSLPWEARLRIASETAGVISYLHSSASTPIIHRDIKTTNIL 561
           EFITNGTL+DH+H  +  D SL WE RL+IA E AG ++YLHSSAS PIIHRDIKT NIL
Sbjct: 495 EFITNGTLFDHLHG-SMIDSSLTWEHRLKIAIEVAGTLAYLHSSASIPIIHRDIKTANIL 554

Query: 562 LDQNYTAKVSDFGASKLVPLDQTQLSTMVQGTLGYLDPEYLLTSELTEKSDVYSFGIVLM 621
           LD N TAKV+DFGAS+L+P+D+ +L TMVQGTLGYLDPEY  T  L EKSDVYSFG+VLM
Sbjct: 555 LDVNLTAKVADFGASRLIPMDKEELETMVQGTLGYLDPEYYNTGLLNEKSDVYSFGVVLM 614

Query: 622 ELITGKKAVSFEGPEAERNLAMYVLCAMKEDRLEEVVEKGMAREGKLEQIKEVAKVAKEC 681
           EL++G+KA+ F+ P++ ++L  Y   A KE+RL+E++   +  E  L++I+E A++A EC
Sbjct: 615 ELLSGQKALCFKRPQSSKHLVSYFATATKENRLDEIIGGEVMNEDNLKEIQEAARIAAEC 674

Query: 682 LRVRGEERPSMKEVAMELEGLRV-LVENKWVNDNNLINAEEMVAYLLEDNNGASES 724
            R+ GEERP MKEVA +LE LRV   ++KW +     N   +  ++L      S S
Sbjct: 675 TRLMGEERPRMKEVAAKLEALRVEKTKHKWSDQYPEENEHLIGGHILSAQGETSSS 717

BLAST of MS015728 vs. TAIR 10
Match: AT1G21240.1 (wall associated kinase 3 )

HSP 1 Score: 587.4 bits (1513), Expect = 1.6e-167
Identity = 335/734 (45.64%), Postives = 457/734 (62.26%), Query Frame = 0

Query: 15  VVQIGILSAAAATAAAGASQALPGCEEWCGDVQIPYPFGMKEGCYL--NEKFFISCNKTH 74
           V  + I   A      G  Q    C+  CG+V I YPFG+  GCY   ++ F ++C    
Sbjct: 7   VFLVVIFFLAYTQLVKGQHQPREDCKLKCGNVTIEYPFGISTGCYYPGDDNFNLTC---- 66

Query: 75  NDSPKAFLQGGNLNVTNISISGELNILHFWARDCYPPNTVPDLSTAF---LNTEFAVSSA 134
               +  L  G + VTNIS SG +++L     +CY      +  TA    L + F++SS 
Sbjct: 67  -VVEEKLLLFGIIQVTNISHSGHVSVLFERFSECYEQKNETN-GTALGYQLGSSFSLSS- 126

Query: 135 KNKFTVIGCDTFSFISGLVGGTQFYKSACVALCDDITTVKDGACSGNGCCQLE---IP-- 194
            NKFT++GC+  S +S    G Q Y + C++LC+      +G C+G GCC  E   +P  
Sbjct: 127 NNKFTLVGCNALSLLSTF--GKQNYSTGCLSLCNS-QPEANGRCNGVGCCTTEDFSVPFD 186

Query: 195 ---------KGLNNLNYTVSSFDNHTNVLSFNPCGYAFVIEEDKFNF-SSKYIRHF-PLE 254
                    +  N +N ++  F  +T+V  FNPC YAF++E+ KFNF SSK +++   + 
Sbjct: 187 SDTFQFGSVRLRNQVNNSLDLF--NTSVYQFNPCTYAFLVEDGKFNFDSSKDLKNLRNVT 246

Query: 255 RVPLVLDWAISNNTCENT-TNCICGPSSQKVNFVEDGSEYRCQCSDGFEGNPYLPRGCQD 314
           R P+ LDW+I N TCE   +  ICG +S   N       Y C+C++G++GNPY   GC+D
Sbjct: 247 RFPVALDWSIGNQTCEQAGSTRICGKNSSCYNSTTRNG-YICKCNEGYDGNPYRSEGCKD 306

Query: 315 VDECKDGRHDCK--FECVNTEGNYTCNCPEGFRGDGRREGEGCTRNSKSFVQIIVGVTVG 374
           +DEC    H+C     C N +G + C CP G+          CTR      +I + + +G
Sbjct: 307 IDECISDTHNCSDPKTCRNRDGGFDCKCPSGY---DLNSSMSCTRPEYKRTRIFLVIIIG 366

Query: 375 FTVLLIGLTWFYLGYRKWKFVKLKEEFFEKNGGLMLQQHLSQWQTSADMVRIFTQEELEK 434
             VLL+         ++ K+ KL+ +FFE+NGG ML Q LS    S    +IFT+E +++
Sbjct: 367 VLVLLLAAICIQHATKQRKYTKLRRQFFEQNGGGMLIQRLSGAGLSNIDFKIFTEEGMKE 426

Query: 435 ATNKYDESAVVGKGGYGTVYKGVLEDGLVVAIKKSKLVDQSQTSQFINEVMVLSQINHRN 494
           ATN YDES ++G+GG GTVYKG+L D  +VAIKK++L D  Q  QFI+EV+VLSQINHRN
Sbjct: 427 ATNGYDESRILGQGGQGTVYKGILPDNTIVAIKKARLADSRQVDQFIHEVLVLSQINHRN 486

Query: 495 VVKLLGCCLETQVPLLVYEFITNGTLYDHIHDKANHDYSLPWEARLRIASETAGVISYLH 554
           VVK+LGCCLET+VPLLVYEFITNGTL+DH+H     D SL WE RLRIA E AG ++YLH
Sbjct: 487 VVKILGCCLETEVPLLVYEFITNGTLFDHLHGSI-FDSSLTWEHRLRIAIEVAGTLAYLH 546

Query: 555 SSASTPIIHRDIKTTNILLDQNYTAKVSDFGASKLVPLDQTQLSTMVQGTLGYLDPEYLL 614
           SSAS PIIHRDIKT NILLD+N TAKV+DFGASKL+P+D+ QL+TMVQGTLGYLDPEY  
Sbjct: 547 SSASIPIIHRDIKTANILLDENLTAKVADFGASKLIPMDKEQLTTMVQGTLGYLDPEYYT 606

Query: 615 TSELTEKSDVYSFGIVLMELITGKKAVSFEGPEAERNLAMYVLCAMKEDRLEEVVEKGMA 674
           T  L EKSDVYSFG+VLMEL++G+KA+ FE P+A ++L  Y + A +E+RL E+++  + 
Sbjct: 607 TGLLNEKSDVYSFGVVLMELLSGQKALCFERPQASKHLVSYFVSATEENRLHEIIDDQVL 666

Query: 675 REGKLEQIKEVAKVAKECLRVRGEERPSMKEVAMELEGLRV-LVENKWVNDNNLINAEEM 724
            E  L++I+E A++A EC R+ GEERP MKEVA +LE LRV   ++KW +     N   +
Sbjct: 667 NEDNLKEIQEAARIAAECTRLMGEERPRMKEVAAKLEALRVEKTKHKWSDQYPEENEHLI 723

BLAST of MS015728 vs. TAIR 10
Match: AT1G21210.1 (wall associated kinase 4 )

HSP 1 Score: 563.1 bits (1450), Expect = 3.2e-160
Identity = 315/690 (45.65%), Postives = 429/690 (62.17%), Query Frame = 0

Query: 34  QALPGCEEWCGDVQIPYPFGMKEGCYLNE--KFFISCNKTHNDSPKAFLQGGNLNVTNIS 93
           Q LP C E CG+V + YPFG   GC+  E   F +SC      +   F +G  L V  IS
Sbjct: 23  QTLPRCPEKCGNVTLEYPFGFSPGCWRAEDPSFNLSC-----VNENLFYKG--LEVVEIS 82

Query: 94  ISGELNILHFWARDCYPPNTVPDLSTAF-LNTEFAVSSAKNKFTVIGCDTFSFISGLVGG 153
            S +L +L+  +  CY         T +  N      S  N  T +GC++++F+S    G
Sbjct: 83  HSSQLRVLYPASYICYNSKGKFAKGTYYWSNLGNLTLSGNNTITALGCNSYAFVSS--NG 142

Query: 154 TQFYKSACVALCDDITTVKDGACSGNGCCQLEIPKGLNNLNYTVSSFDNHTNV--LSFNP 213
           T+     C++ CD ++   +G C+G GCCQ  +P G N L      FDN T+V  +S   
Sbjct: 143 TRRNSVGCISACDALSHEANGECNGEGCCQNPVPAGNNWLIVRSYRFDNDTSVQPISEGQ 202

Query: 214 CGYAFVIEEDKFNFSSKYIRHFPLER---VPLVLDWAISNNTCENTTNCICGPSSQKVNF 273
           C YAF++E  KF +++     +   R    P+VLDW+I   TC       CG +    N 
Sbjct: 203 CIYAFLVENGKFKYNASDKYSYLQNRNVGFPVVLDWSIRGETCGQVGEKKCGVNGICSNS 262

Query: 274 VEDGSEYRCQCSDGFEGNPYLPRGCQDVDECKDG----RHDCKFE--CVNTEGNYTCNCP 333
              G  Y C+C  GF+GNPYL  GCQD++EC       +H+C  +  C N  G++ CNC 
Sbjct: 263 A-SGIGYTCKCKGGFQGNPYLQNGCQDINECTTANPIHKHNCSGDSTCENKLGHFRCNC- 322

Query: 334 EGFRGDGRREGEGCTRNSK--------SFVQIIVGVTVGFTVLLIGLTWFYLGYRKWKFV 393
                  R E    T   K         +  I++G T+GF V+L+ ++      +  K  
Sbjct: 323 -----RSRYELNTTTNTCKPKGNPEYVEWTTIVLGTTIGFLVILLAISCIEHKMKNTKDT 382

Query: 394 KLKEEFFEKNGGLMLQQHLSQWQTSADMVRIFTQEELEKATNKYDESAVVGKGGYGTVYK 453
           +L+++FFE+NGG ML Q LS    S   V+IFT+E +++AT+ YDE+ ++G+GG GTVYK
Sbjct: 383 ELRQQFFEQNGGGMLMQRLSGAGPSNVDVKIFTEEGMKEATDGYDENRILGQGGQGTVYK 442

Query: 454 GVLEDGLVVAIKKSKLVDQSQTSQFINEVMVLSQINHRNVVKLLGCCLETQVPLLVYEFI 513
           G+L D  +VAIKK++L D SQ  QFINEV+VLSQINHRNVVKLLGCCLET+VPLLVYEFI
Sbjct: 443 GILPDNSIVAIKKARLGDNSQVEQFINEVLVLSQINHRNVVKLLGCCLETEVPLLVYEFI 502

Query: 514 TNGTLYDHIHDKANHDYSLPWEARLRIASETAGVISYLHSSASTPIIHRDIKTTNILLDQ 573
           ++GTL+DH+H  +  D SL WE RLR+A E AG ++YLHSSAS PIIHRDIKT NILLD+
Sbjct: 503 SSGTLFDHLHG-SMFDSSLTWEHRLRMAVEIAGTLAYLHSSASIPIIHRDIKTANILLDE 562

Query: 574 NYTAKVSDFGASKLVPLDQTQLSTMVQGTLGYLDPEYLLTSELTEKSDVYSFGIVLMELI 633
           N TAKV+DFGAS+L+P+D+  L+TMVQGTLGYLDPEY  T  L EKSDVYSFG+VLMEL+
Sbjct: 563 NLTAKVADFGASRLIPMDKEDLATMVQGTLGYLDPEYYNTGLLNEKSDVYSFGVVLMELL 622

Query: 634 TGKKAVSFEGPEAERNLAMYVLCAMKEDRLEEVVEKGMAREGKLEQIKEVAKVAKECLRV 693
           +G+KA+ FE P+  +++  Y   A KE+RL E+++  +  E    +I++ A++A EC R+
Sbjct: 623 SGQKALCFERPQTSKHIVSYFASATKENRLHEIIDGQVMNENNQREIQKAARIAVECTRL 682

Query: 694 RGEERPSMKEVAMELEGLRVL-VENKWVND 701
            GEERP MKEVA ELE LRV   ++KW ++
Sbjct: 683 TGEERPGMKEVAAELEALRVTKTKHKWSDE 695

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022141581.10.0e+0099.60putative wall-associated receptor kinase-like 16 [Momordica charantia][more]
XP_023512416.10.0e+0075.66wall-associated receptor kinase 2-like [Cucurbita pepo subsp. pepo][more]
XP_022960687.10.0e+0075.36wall-associated receptor kinase 3-like [Cucurbita moschata][more]
XP_022989770.10.0e+0074.80wall-associated receptor kinase 2-like [Cucurbita maxima][more]
XP_038884307.10.0e+0073.49wall-associated receptor kinase 2-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q9LMP12.7e-17548.30Wall-associated receptor kinase 2 OS=Arabidopsis thaliana OX=3702 GN=WAK2 PE=1 S... [more]
Q9LMN75.7e-17046.61Wall-associated receptor kinase 5 OS=Arabidopsis thaliana OX=3702 GN=WAK5 PE=2 S... [more]
Q391912.8e-16946.23Wall-associated receptor kinase 1 OS=Arabidopsis thaliana OX=3702 GN=WAK1 PE=1 S... [more]
Q9LMN82.3e-16645.64Wall-associated receptor kinase 3 OS=Arabidopsis thaliana OX=3702 GN=WAK3 PE=2 S... [more]
Q9LMN64.6e-15945.65Wall-associated receptor kinase 4 OS=Arabidopsis thaliana OX=3702 GN=WAK4 PE=2 S... [more]
Match NameE-valueIdentityDescription
A0A6J1CJM00.0e+0099.60putative wall-associated receptor kinase-like 16 OS=Momordica charantia OX=3673 ... [more]
A0A6J1H8430.0e+0075.36wall-associated receptor kinase 3-like OS=Cucurbita moschata OX=3662 GN=LOC11146... [more]
A0A6J1JNA50.0e+0074.80wall-associated receptor kinase 2-like OS=Cucurbita maxima OX=3661 GN=LOC1114868... [more]
A0A6J1CJ332.5e-30172.89putative wall-associated receptor kinase-like 16 OS=Momordica charantia OX=3673 ... [more]
A0A1S3B3B12.5e-30169.83wall-associated receptor kinase 2-like OS=Cucumis melo OX=3656 GN=LOC103485676 P... [more]
Match NameE-valueIdentityDescription
AT1G21270.11.9e-17648.30wall-associated kinase 2 [more]
AT1G21230.14.1e-17146.61wall associated kinase 5 [more]
AT1G21250.12.0e-17046.23cell wall-associated kinase [more]
AT1G21240.11.6e-16745.64wall associated kinase 3 [more]
AT1G21210.13.2e-16045.65wall associated kinase 4 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 415..693
e-value: 7.0E-32
score: 121.9
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 415..698
score: 39.156139
IPR000742EGF-like domainSMARTSM00181egf_5coord: 247..291
e-value: 53.0
score: 7.7
coord: 295..334
e-value: 0.013
score: 24.7
IPR000742EGF-like domainPROSITEPS50026EGF_3coord: 292..325
score: 9.157378
IPR001881EGF-like calcium-binding domainSMARTSM00179egfca_6coord: 292..334
e-value: 7.6E-7
score: 38.7
IPR001881EGF-like calcium-binding domainPFAMPF07645EGF_CAcoord: 292..326
e-value: 5.3E-8
score: 32.9
NoneNo IPR availableGENE3D2.10.25.10Laminincoord: 243..333
e-value: 8.3E-13
score: 50.5
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 491..709
e-value: 5.9E-58
score: 197.6
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 386..490
e-value: 3.5E-31
score: 109.4
NoneNo IPR availablePANTHERPTHR27005:SF341WALL-ASSOCIATED RECEPTOR KINASE 2-LIKE ISOFORM X2coord: 19..711
NoneNo IPR availablePANTHERPTHR27005WALL-ASSOCIATED RECEPTOR KINASE-LIKE 21coord: 19..711
NoneNo IPR availableCDDcd00054EGF_CAcoord: 292..325
e-value: 1.39119E-7
score: 46.0906
NoneNo IPR availableCDDcd14066STKc_IRAKcoord: 421..689
e-value: 3.81421E-98
score: 302.655
NoneNo IPR availableSUPERFAMILY57196EGF/Laminincoord: 290..326
IPR001245Serine-threonine/tyrosine-protein kinase, catalytic domainPFAMPF07714PK_Tyr_Ser-Thrcoord: 420..686
e-value: 8.2E-48
score: 162.9
IPR025287Wall-associated receptor kinase, galacturonan-binding domainPFAMPF13947GUB_WAK_bindcoord: 39..94
e-value: 8.5E-9
score: 35.7
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 537..549
IPR000152EGF-type aspartate/asparagine hydroxylation sitePROSITEPS00010ASX_HYDROXYLcoord: 307..318
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 421..444
IPR018097EGF-like calcium-binding, conserved sitePROSITEPS01187EGF_CAcoord: 292..316
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 393..687

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS015728.1MS015728.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
molecular_function GO:0005524 ATP binding
molecular_function GO:0005509 calcium ion binding
molecular_function GO:0030247 polysaccharide binding
molecular_function GO:0004672 protein kinase activity