MS015671 (gene) Bitter gourd (TR) v1

BLAST of MS015671 vs. NCBI nr
Match: XP_022993952.1 (nodulation-signaling pathway 2 protein [Cucurbita maxima])

HSP 1 Score: 631.7 bits (1628), Expect = 4.8e-177
Identity = 333/453 (73.51%), Postives = 376/453 (83.00%), Query Frame = 0

Query: 17  DFHDLIDSMMDADVPTYA---AEVDGSSNCNSEEEESTEAEDQEEELKGLRLYHLLMAAA 76
           DFHDL DS+M  D   Y+    EVDG +NCNS    + + E++EEE K LRLYHLLMA A
Sbjct: 27  DFHDLFDSIMSIDALPYSPQVVEVDGGTNCNSPSNPAEDREEEEEEFKSLRLYHLLMAVA 86

Query: 77  DALYGEHKSRDLAHVILLRLNELVSPSHGTNLERLAAYYTQALQGLLECTA---GVGKSH 136
           DA++G++KSR+LA VIL+RLNELVS SHGTNLERL AYY QA Q LL+C A   GV    
Sbjct: 87  DAMFGDNKSRELAQVILIRLNELVSTSHGTNLERLTAYYAQAFQDLLDCAAVSGGVPNKP 146

Query: 137 HHLHRDDHTPGDVVAAFQLLQEMSPYVKFGHFTANQAILEAVGDDRRVHIVDYDIMEGIQ 196
           HHLHRDDH+P DV+AAFQLLQ+MSPYVKFGHFTANQAILEAV DDRRVHIVDY+IMEGIQ
Sbjct: 147 HHLHRDDHSPTDVLAAFQLLQDMSPYVKFGHFTANQAILEAVADDRRVHIVDYNIMEGIQ 206

Query: 197 WASLMQAFVSRKDGPPAPHLRITAISRGGSTARRSIGTVQETGRRLVAFAASIGQPFSFH 256
           WASLMQAFVSRKD PPAPHLRIT ISRG S +RR IGTVQETGRRLVAFAASIGQPFSFH
Sbjct: 207 WASLMQAFVSRKDSPPAPHLRITVISRGPS-SRRWIGTVQETGRRLVAFAASIGQPFSFH 266

Query: 257 QCKLESDESFRAAGLKLVKGEAVVVNCMLHLPHFRHRTPESIGSFLRGAEALSPRVVCLV 316
           QCKL+SDESFR +GLKLV+GEA+VVNC+LHLPHF + +PESI SFL GA+ L+PR+V LV
Sbjct: 267 QCKLDSDESFRPSGLKLVRGEALVVNCVLHLPHFSNSSPESIASFLAGAKTLNPRLVTLV 326

Query: 317 EEEIGHG---IGDYKVEFVESLERYSAIYESLEVGLPMNNRGRALVERVFLGPQIWGSVG 376
           EEEI HG    GDYKV+F++SLERYSAIY+SLE G+PM NR R LVE+VFLGP+I  ++ 
Sbjct: 327 EEEIEHGPTIDGDYKVQFLDSLERYSAIYDSLEAGIPMKNRARGLVEKVFLGPRISATLR 386

Query: 377 RMGQPRTAAGS------WGEWLGKMGMKAAPISFANHCQARLLVGLFNDGYIVEELSNNK 436
           R+GQP+ AA +      WGE L KMG+KAA ISFANHCQARLLV LFNDGY VEEL +NK
Sbjct: 387 RIGQPQRAATAAEENCLWGERLEKMGLKAAAISFANHCQARLLVDLFNDGYRVEELGSNK 446

Query: 437 LVLGWKSKRLLSASIWASSSSPSSSSSSSSDSE 455
           LVLGWKSKRLLS SIWASSS  SSSSSSSSDSE
Sbjct: 447 LVLGWKSKRLLSVSIWASSS--SSSSSSSSDSE 476

BLAST of MS015671 vs. NCBI nr
Match: XP_023551167.1 (nodulation-signaling pathway 2 protein isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 627.5 bits (1617), Expect = 9.1e-176
Identity = 333/453 (73.51%), Postives = 373/453 (82.34%), Query Frame = 0

Query: 17  DFHDLIDSMMDADVPTYA---AEVDGSSNCNSEEEESTEAEDQEEELKGLRLYHLLMAAA 76
           DFHDL DS+M  D   Y+    EVDG +NCNS    + + E++EEE K LRLYHLLMA A
Sbjct: 27  DFHDLFDSIMSIDALPYSPQVVEVDGGTNCNSPSNPAEDREEEEEEFKSLRLYHLLMAVA 86

Query: 77  DALYGEHKSRDLAHVILLRLNELVSPSHGTNLERLAAYYTQALQGLLECTA---GVGKSH 136
           DA++G+HKSRDLA VIL+RLNELVS SHGTNLERL AYY Q+ Q LL+  A   G     
Sbjct: 87  DAMFGDHKSRDLAQVILIRLNELVSTSHGTNLERLTAYYAQSFQDLLDYAAVSGGGSNKP 146

Query: 137 HHLHRDDHTPGDVVAAFQLLQEMSPYVKFGHFTANQAILEAVGDDRRVHIVDYDIMEGIQ 196
           HHLHRDDH+P DV+AAFQLLQEMSPYVKFGHFTANQAILEAV DDRRVHIVDY+IMEGIQ
Sbjct: 147 HHLHRDDHSPTDVLAAFQLLQEMSPYVKFGHFTANQAILEAVADDRRVHIVDYNIMEGIQ 206

Query: 197 WASLMQAFVSRKDGPPAPHLRITAISRGGSTARRSIGTVQETGRRLVAFAASIGQPFSFH 256
           WASLMQA VSRKD PPAPHLRITAISRG S +RR IGTVQETGRRLVAFAASIGQPFSFH
Sbjct: 207 WASLMQALVSRKDSPPAPHLRITAISRGPS-SRRWIGTVQETGRRLVAFAASIGQPFSFH 266

Query: 257 QCKLESDESFRAAGLKLVKGEAVVVNCMLHLPHFRHRTPESIGSFLRGAEALSPRVVCLV 316
           QCKL+SDESFR +GLKLV+GEA+VVNC+LHLPHF + +PESI SFL GA+ L+PR+V LV
Sbjct: 267 QCKLDSDESFRPSGLKLVRGEALVVNCVLHLPHFSNTSPESIDSFLAGAKTLNPRLVTLV 326

Query: 317 EEEIGHG---IGDYKVEFVESLERYSAIYESLEVGLPMNNRGRALVERVFLGPQIWGSVG 376
           EEEI HG    GDYKV+F++SLERYSAIY+SLE G+PM NR R LVE+VFLGP+I  ++ 
Sbjct: 327 EEEIEHGPTIDGDYKVQFLDSLERYSAIYDSLEAGIPMKNRARGLVEKVFLGPRISATLK 386

Query: 377 RMGQPRTAAG------SWGEWLGKMGMKAAPISFANHCQARLLVGLFNDGYIVEELSNNK 436
           R+GQP+ AA       SWGE L KMG+KAA ISFANHCQARLLV LFNDGY VEEL +NK
Sbjct: 387 RIGQPQRAATAAEENCSWGERLEKMGLKAAAISFANHCQARLLVDLFNDGYRVEELGSNK 446

Query: 437 LVLGWKSKRLLSASIWASSSSPSSSSSSSSDSE 455
           LVLGWKSKRLLS SIWASSS   SSSSSSSDSE
Sbjct: 447 LVLGWKSKRLLSVSIWASSS--LSSSSSSSDSE 476

BLAST of MS015671 vs. NCBI nr
Match: KAG6578977.1 (Protein NODULATION SIGNALING PATHWAY 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 626.7 bits (1615), Expect = 1.6e-175
Identity = 331/453 (73.07%), Postives = 373/453 (82.34%), Query Frame = 0

Query: 17  DFHDLIDSMMDADVPTYA---AEVDGSSNCNSEEEESTEAEDQEEELKGLRLYHLLMAAA 76
           DFHDL DS+M  D   Y+    E+DG +NCNS    +   E++EEE + LRLYHLLMA A
Sbjct: 27  DFHDLFDSIMSIDALPYSPQVVEIDGGTNCNSPSNPAEGREEEEEEFRSLRLYHLLMAVA 86

Query: 77  DALYGEHKSRDLAHVILLRLNELVSPSHGTNLERLAAYYTQALQGLLECTA---GVGKSH 136
           DA++G+HKSR+LA VIL+RLNELVS SHGTNLERL AYY QA Q LL+C A   G     
Sbjct: 87  DAMFGDHKSRELAQVILIRLNELVSTSHGTNLERLTAYYAQAFQDLLDCAAVSGGGPNKP 146

Query: 137 HHLHRDDHTPGDVVAAFQLLQEMSPYVKFGHFTANQAILEAVGDDRRVHIVDYDIMEGIQ 196
           HHLHRDDH+P DV+AAFQLLQEMSPYVKFGHFTANQAILEAV DDRRVHIVDY+IMEGIQ
Sbjct: 147 HHLHRDDHSPTDVLAAFQLLQEMSPYVKFGHFTANQAILEAVADDRRVHIVDYNIMEGIQ 206

Query: 197 WASLMQAFVSRKDGPPAPHLRITAISRGGSTARRSIGTVQETGRRLVAFAASIGQPFSFH 256
           WASLMQA VSRKD PPAPHLRITAISRG S +RR IGTVQETGRRLVAFAASIGQPFSFH
Sbjct: 207 WASLMQALVSRKDSPPAPHLRITAISRGPS-SRRWIGTVQETGRRLVAFAASIGQPFSFH 266

Query: 257 QCKLESDESFRAAGLKLVKGEAVVVNCMLHLPHFRHRTPESIGSFLRGAEALSPRVVCLV 316
           QCKL+SDESFR +GLKLV+GEA+VVNC+LHLPHF + +PESI SFL GA+ L+PR+V LV
Sbjct: 267 QCKLDSDESFRPSGLKLVRGEALVVNCVLHLPHFSNTSPESIASFLAGAKTLNPRLVTLV 326

Query: 317 EEEIGHG---IGDYKVEFVESLERYSAIYESLEVGLPMNNRGRALVERVFLGPQIWGSVG 376
           EEEI HG    GDYKV+F++SLERYSAIY+SLE G+PM NR R LVE+VFLGP+I  ++ 
Sbjct: 327 EEEIEHGPTIDGDYKVQFLDSLERYSAIYDSLEAGIPMKNRARGLVEKVFLGPRISATLR 386

Query: 377 RMGQPRTAAGS------WGEWLGKMGMKAAPISFANHCQARLLVGLFNDGYIVEELSNNK 436
           R+GQP+ AA +      WGE L KMG+KAA ISFANHCQARLLV LFNDGY VEEL +NK
Sbjct: 387 RIGQPQRAATAAEENCLWGERLEKMGLKAAAISFANHCQARLLVDLFNDGYRVEELGSNK 446

Query: 437 LVLGWKSKRLLSASIWASSSSPSSSSSSSSDSE 455
           LVLGWKSKRLLS SIWASSS   SSSSSSSDSE
Sbjct: 447 LVLGWKSKRLLSVSIWASSS--LSSSSSSSDSE 476

BLAST of MS015671 vs. NCBI nr
Match: KAG7016501.1 (Nodulation-signaling pathway 2 protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 625.9 bits (1613), Expect = 2.7e-175
Identity = 330/453 (72.85%), Postives = 373/453 (82.34%), Query Frame = 0

Query: 17  DFHDLIDSMMDADVPTYA---AEVDGSSNCNSEEEESTEAEDQEEELKGLRLYHLLMAAA 76
           DFHDL DS+M  D   Y+    E+DG +NCNS    +   E++EEE + LRLYHLLMA A
Sbjct: 27  DFHDLFDSIMSIDALPYSPQVVEIDGGTNCNSPSNPAEGREEEEEEFRSLRLYHLLMAVA 86

Query: 77  DALYGEHKSRDLAHVILLRLNELVSPSHGTNLERLAAYYTQALQGLLECTA---GVGKSH 136
           DA++G+HKSR+LA VIL+RLNELVS SHGTNLER+ AYY QA Q LL+C A   G     
Sbjct: 87  DAMFGDHKSRELAQVILIRLNELVSTSHGTNLERITAYYAQAFQDLLDCAAVSGGGPNKP 146

Query: 137 HHLHRDDHTPGDVVAAFQLLQEMSPYVKFGHFTANQAILEAVGDDRRVHIVDYDIMEGIQ 196
           HHLHRDDH+P DV+AAFQLLQEMSPYVKFGHFTANQAILEAV DDRRVHIVDY+IMEGIQ
Sbjct: 147 HHLHRDDHSPTDVLAAFQLLQEMSPYVKFGHFTANQAILEAVADDRRVHIVDYNIMEGIQ 206

Query: 197 WASLMQAFVSRKDGPPAPHLRITAISRGGSTARRSIGTVQETGRRLVAFAASIGQPFSFH 256
           WASLMQA VSRKD PPAPHLRITAISRG S +RR IGTVQETGRRLVAFAASIGQPFSFH
Sbjct: 207 WASLMQALVSRKDSPPAPHLRITAISRGPS-SRRWIGTVQETGRRLVAFAASIGQPFSFH 266

Query: 257 QCKLESDESFRAAGLKLVKGEAVVVNCMLHLPHFRHRTPESIGSFLRGAEALSPRVVCLV 316
           QCKL+SDESFR +GLKLV+GEA+VVNC+LHLPHF + +PESI SFL GA+ L+PR+V LV
Sbjct: 267 QCKLDSDESFRPSGLKLVRGEALVVNCVLHLPHFSNTSPESIASFLAGAKTLNPRLVTLV 326

Query: 317 EEEIGHG---IGDYKVEFVESLERYSAIYESLEVGLPMNNRGRALVERVFLGPQIWGSVG 376
           EEEI HG    GDYKV+F++SLERYSAIY+SLE G+PM NR R LVE+VFLGP+I  ++ 
Sbjct: 327 EEEIEHGPTIDGDYKVQFLDSLERYSAIYDSLEAGIPMKNRARGLVEKVFLGPRISATLR 386

Query: 377 RMGQPRTAAGS------WGEWLGKMGMKAAPISFANHCQARLLVGLFNDGYIVEELSNNK 436
           R+GQP+ AA +      WGE L KMG+KAA ISFANHCQARLLV LFNDGY VEEL +NK
Sbjct: 387 RIGQPQRAATAAEENCLWGERLEKMGLKAAAISFANHCQARLLVDLFNDGYRVEELGSNK 446

Query: 437 LVLGWKSKRLLSASIWASSSSPSSSSSSSSDSE 455
           LVLGWKSKRLLS SIWASSS   SSSSSSSDSE
Sbjct: 447 LVLGWKSKRLLSVSIWASSS--LSSSSSSSDSE 476

BLAST of MS015671 vs. NCBI nr
Match: XP_022938560.1 (nodulation-signaling pathway 2 protein [Cucurbita moschata])

HSP 1 Score: 622.9 bits (1605), Expect = 2.2e-174
Identity = 329/453 (72.63%), Postives = 372/453 (82.12%), Query Frame = 0

Query: 17  DFHDLIDSMMDADVPTYA---AEVDGSSNCNSEEEESTEAEDQEEELKGLRLYHLLMAAA 76
           DFHDL DS+M  D   Y+    E+DG +NCNS    +   E++EEE + LRLYHLLMA A
Sbjct: 27  DFHDLFDSIMSIDALPYSPQVVEIDGGTNCNSPSNPAEGREEEEEEFRSLRLYHLLMAVA 86

Query: 77  DALYGEHKSRDLAHVILLRLNELVSPSHGTNLERLAAYYTQALQGLLECTA---GVGKSH 136
           DA++G+HKSR+LA VIL+RLNELVS SHGTNLER+ AYY QA Q LL+C A   G     
Sbjct: 87  DAMFGDHKSRELAQVILIRLNELVSTSHGTNLERITAYYAQAFQDLLDCAAVSGGGPNKP 146

Query: 137 HHLHRDDHTPGDVVAAFQLLQEMSPYVKFGHFTANQAILEAVGDDRRVHIVDYDIMEGIQ 196
           HHLHRDDH+P DV+AAFQLLQEMSPYVKFGHFTANQAILEAV DDRRVHIVDY+IMEGIQ
Sbjct: 147 HHLHRDDHSPTDVLAAFQLLQEMSPYVKFGHFTANQAILEAVADDRRVHIVDYNIMEGIQ 206

Query: 197 WASLMQAFVSRKDGPPAPHLRITAISRGGSTARRSIGTVQETGRRLVAFAASIGQPFSFH 256
           WASLMQA VSRKD PPAPHLRITAISRG S +RR IGTVQETGRRLVAFAASIGQPFSFH
Sbjct: 207 WASLMQALVSRKDSPPAPHLRITAISRGPS-SRRWIGTVQETGRRLVAFAASIGQPFSFH 266

Query: 257 QCKLESDESFRAAGLKLVKGEAVVVNCMLHLPHFRHRTPESIGSFLRGAEALSPRVVCLV 316
           QCKL+SDESFR +GLKLV+GEA+VVNC+LHLPHF + +PESI SFL GA+ L+PR+V LV
Sbjct: 267 QCKLDSDESFRPSGLKLVRGEALVVNCVLHLPHFSNTSPESIASFLAGAKTLNPRLVTLV 326

Query: 317 EEEIGHG---IGDYKVEFVESLERYSAIYESLEVGLPMNNRGRALVERVFLGPQIWGSVG 376
           EEEI HG    GDYKV+F++SLERYSAIY+SLE G+PM NR R LVE+VFLGP+I  ++ 
Sbjct: 327 EEEIEHGPTIDGDYKVQFLDSLERYSAIYDSLEAGIPMKNRARGLVEKVFLGPRISATLR 386

Query: 377 RMGQPRTAAGS------WGEWLGKMGMKAAPISFANHCQARLLVGLFNDGYIVEELSNNK 436
           R+ QP+ AA +      WGE L KMG+KAA ISFANHCQARLLV LFNDGY VEEL +NK
Sbjct: 387 RIEQPQRAATAAEENCLWGERLEKMGLKAAAISFANHCQARLLVDLFNDGYRVEELGSNK 446

Query: 437 LVLGWKSKRLLSASIWASSSSPSSSSSSSSDSE 455
           LVLGWKSKRLLS SIWASSS   SSSSSSSDSE
Sbjct: 447 LVLGWKSKRLLSVSIWASSS--LSSSSSSSDSE 476

BLAST of MS015671 vs. ExPASy Swiss-Prot
Match: Q2PEG7 (Protein NODULATION SIGNALING PATHWAY 2 OS=Lotus japonicus OX=34305 GN=NSP2 PE=1 SV=1)

HSP 1 Score: 502.3 bits (1292), Expect = 5.8e-141
Identity = 272/465 (58.49%), Postives = 332/465 (71.40%), Query Frame = 0

Query: 1   DYCSPDADWEAFPG-AGDFHDLIDSMMDADVPTYAAEVDGSSNCNSEEEESTEA------ 60
           ++ SP  +W+AF G   DFH LIDSM+D D  T  A  D +++  SEEEE  EA      
Sbjct: 35  NHWSPVVNWDAFTGNQDDFHHLIDSMID-DNNTGPAFSDHTASTTSEEEEEEEATTTTMT 94

Query: 61  ----------EDQEEELKGLRLYHLLMAAADALYGEHKSRDLAHVILLRLNELVSPSHGT 120
                     E  +++ KGLRL HLLMA A+AL G +K+R+LA VIL+RL ELVS + GT
Sbjct: 95  TTTTTTTTTPEAADDDFKGLRLVHLLMAGAEALTGANKNRELARVILVRLKELVSHTDGT 154

Query: 121 NLERLAAYYTQALQGLLECTAGVGKS---HHHLHRDDHTP-GDVVAAFQLLQEMSPYVKF 180
           N+ERLAAY+T+ALQGLLE   G   S   HH +    H P  D +AAFQLLQ+MSPYVKF
Sbjct: 155 NMERLAAYFTEALQGLLEGAGGAYNSSSKHHVIGGPHHEPQNDALAAFQLLQDMSPYVKF 214

Query: 181 GHFTANQAILEAVGDDRRVHIVDYDIMEGIQWASLMQAFVSRKDGPPAPHLRITAISRGG 240
           GHFTANQAI+EAV  +RRVHIVDYDIMEG+QWASLMQA  S  +G   PHLRITA+SR G
Sbjct: 215 GHFTANQAIVEAVAHERRVHIVDYDIMEGVQWASLMQALASNPNG---PHLRITALSRSG 274

Query: 241 STARRSIGTVQETGRRLVAFAASIGQPFSFHQCKLESDESFRAAGLKLVKGEAVVVNCML 300
              RRS+ TVQETGRRL AFA S+GQPFSFH  +LESDE+FR AGLKLV+GEA+V NCML
Sbjct: 275 -VGRRSMATVQETGRRLTAFATSLGQPFSFHHSRLESDETFRPAGLKLVRGEALVFNCML 334

Query: 301 HLPHFRHRTPESIGSFLRGAEALSPRVVCLVEEEIGHGIGDYKVEFVESLERYSAIYESL 360
           +LPH  +R+P S+ SFL  A+AL PR+V +VEEE+G  +G +   F++SL  +SA+++SL
Sbjct: 335 NLPHLTYRSPNSVASFLTAAKALRPRLVTVVEEEVGSALGGFVERFMDSLHHFSAVFDSL 394

Query: 361 EVGLPMNNRGRALVERVFLGPQIWGSVGRM---GQPRTAAGSWGEWLGKMGMKAAPISFA 420
           E G PM  R RALVERVFLGP+I GS+ R+   G      GSW EWL   G     +S A
Sbjct: 395 EAGFPMQGRARALVERVFLGPRIVGSLARIYRTGGGGEERGSWREWLRAAGFSGVAVSSA 454

Query: 421 NHCQARLLVGLFNDGYIVEELSNNKLVLGWKSKRLLSASIWASSS 442
           NHCQ+ LL+GLFNDGY VEEL +NKLVL WK++RLLSAS+W  SS
Sbjct: 455 NHCQSNLLLGLFNDGYRVEELGSNKLVLHWKTRRLLSASLWTCSS 494

BLAST of MS015671 vs. ExPASy Swiss-Prot
Match: Q5NE24 (Protein NODULATION SIGNALING PATHWAY 2 OS=Medicago truncatula OX=3880 GN=NSP2 PE=1 SV=1)

HSP 1 Score: 498.0 bits (1281), Expect = 1.1e-139
Identity = 262/477 (54.93%), Postives = 341/477 (71.49%), Query Frame = 0

Query: 1   DYCSPDADWEAFPGA-GDFHDLIDSMMDADVPTYAAEVDGSSNCNSEEEESTEAEDQE-- 60
           ++ SP  +W+ F GA  DFH L+D++++ D  T   ++  S    +    +T+ E++E  
Sbjct: 35  NHWSPIVNWDTFTGAPDDFHHLMDTIIE-DRTTVLEQLSPSITTTTTTTTTTDEEEEEME 94

Query: 61  ----------------EELKGLRLYHLLMAAADALYGEHKSRDLAHVILLRLNELVSP-S 120
                           ++ KGL+L HLLMA A+AL G  K+RDLA VIL+RL ELVS  +
Sbjct: 95  TTTTTTTTAIKTHEVGDDSKGLKLVHLLMAGAEALTGSTKNRDLARVILIRLKELVSQHA 154

Query: 121 HGTNLERLAAYYTQALQGLLECTAGVGKSHHHLHRDDH---------TPGDVVAAFQLLQ 180
           +G+N+ERLAA++T+AL GLLE   G   +HHH + + H            D +AAFQLLQ
Sbjct: 155 NGSNMERLAAHFTEALHGLLEGAGGAHNNHHHHNNNKHYLTTNGPHDNQNDTLAAFQLLQ 214

Query: 181 EMSPYVKFGHFTANQAILEAVGDDRRVHIVDYDIMEGIQWASLMQAFVSRKDGPPAPHLR 240
           +MSPYVKFGHFTANQAI+EAV  +RRVH++DYDIMEG+QWASL+Q+  S  +G   PHLR
Sbjct: 215 DMSPYVKFGHFTANQAIIEAVAHERRVHVIDYDIMEGVQWASLIQSLASNNNG---PHLR 274

Query: 241 ITAISRGGSTARRSIGTVQETGRRLVAFAASIGQPFSFHQCKLESDESFRAAGLKLVKGE 300
           ITA+SR G T RRSI TVQETGRRL +FAAS+GQPFSFH C+L+SDE+FR + LKLV+GE
Sbjct: 275 ITALSRTG-TGRRSIATVQETGRRLTSFAASLGQPFSFHHCRLDSDETFRPSALKLVRGE 334

Query: 301 AVVVNCMLHLPHFRHRTPESIGSFLRGAEALSPRVVCLVEEEIGHGIGDYKVEFVESLER 360
           A+V NCML+LPH  +R PES+ SFL GA+ L+P++V LVEEE+G  IG +   F++SL  
Sbjct: 335 ALVFNCMLNLPHLSYRAPESVASFLNGAKTLNPKLVTLVEEEVGSVIGGFVERFMDSLHH 394

Query: 361 YSAIYESLEVGLPMNNRGRALVERVFLGPQIWGSVGRMGQP--RTAAGSWGEWLGKMGMK 420
           YSA+++SLE G PM NR R LVERVF GP+I GS+GR+ +        SWGEWLG++G +
Sbjct: 395 YSAVFDSLEAGFPMQNRARTLVERVFFGPRIAGSLGRIYRTGGEEERRSWGEWLGEVGFR 454

Query: 421 AAPISFANHCQARLLVGLFNDGYIVEE--LSNNKLVLGWKSKRLLSASIWASSSSPS 445
             P+SFANHCQA+LL+GLFNDGY VEE  + +NKLVL WKS+RLLSAS+W  SSS S
Sbjct: 455 GVPVSFANHCQAKLLLGLFNDGYRVEEVGVGSNKLVLDWKSRRLLSASLWTCSSSDS 506

BLAST of MS015671 vs. ExPASy Swiss-Prot
Match: Q84Q92 (Protein NODULATION SIGNALING PATHWAY 2 OS=Oryza sativa subsp. japonica OX=39947 GN=NSP2 PE=1 SV=1)

HSP 1 Score: 416.8 bits (1070), Expect = 3.2e-115
Identity = 254/504 (50.40%), Postives = 306/504 (60.71%), Query Frame = 0

Query: 4   SPDADWEAF-----PGAGDFHDLIDSMMDADVPTYAAEVDGSSNC--------------N 63
           SP ADW  F      G  D H LI+SM+  D      + DG +                N
Sbjct: 43  SPVADWANFCCSDDDGGHDLHGLIESML-CDDTLVGVDDDGQAGLHHADMFRDDLYCYGN 102

Query: 64  SEEEESTEAEDQEEEL----------KGLRLYHLLMAAADALYGEHKSRDLAHVILLRLN 123
                ST   +    +          KGLRL HLLMAAA+AL G HKSR+LA VIL+RL 
Sbjct: 103 GSNPSSTTTTNPGSPVFDDPTQGCPEKGLRLLHLLMAAAEALSGPHKSRELARVILVRLK 162

Query: 124 ELVS-----PSHGTNLERLAAYYTQALQGLLECTAGVG---------KSHHHLHRDDHTP 183
           E+VS      +  +N+ERLAA++T ALQGLL+ +  VG          SHHH        
Sbjct: 163 EMVSHTASANAAASNMERLAAHFTDALQGLLDGSHPVGGSGRQAAAAASHHH-------A 222

Query: 184 GDVVAAFQLLQEMSPYVKFGHFTANQAILEAVGDDRRVHIVDYDIMEGIQWASLMQAFVS 243
           GDV+ AFQ+LQ+MSPY+KFGHFTANQAILEAV  DRRVHIVDYDI EGIQWASLMQA  S
Sbjct: 223 GDVLTAFQMLQDMSPYMKFGHFTANQAILEAVSGDRRVHIVDYDIAEGIQWASLMQAMTS 282

Query: 244 RKDGPPAPHLRITAISRGGSTARRSIGTVQETGRRLVAFAASIGQPFSFHQCKLESDESF 303
           R DG PAPHLRITA+SR G    R+   VQE GRRL AFAASIGQPFSF QC+L+SDE F
Sbjct: 283 RADGVPAPHLRITAVSRSGGGGARA---VQEAGRRLSAFAASIGQPFSFGQCRLDSDERF 342

Query: 304 RAAGLKLVKGEAVVVNCMLHLPHFR---HRTPESIGSFLRGAEALSPRVVCLVEEE---- 363
           R A +++VKGEA+V NC+LH         R   S+ SFL G  AL  ++V +VEEE    
Sbjct: 343 RPATVRMVKGEALVANCVLHQAAATTTIRRPTGSVASFLSGMAALGAKLVTVVEEEGEAE 402

Query: 364 ---IGHGIGD-----YKVEFVESLERYSAIYESLEVGLPMNNRGRALVERVFLGPQIWGS 423
               G   GD     +  +F+E L RYSA+++SLE G P  +R R LVERV L P I G+
Sbjct: 403 KDDDGDSAGDAAAGGFVRQFMEELHRYSAVWDSLEAGFPTQSRVRGLVERVILAPNIAGA 462

Query: 424 VGRMGQPRTAAG--SWGEWLGKMGMKAAPISFANHCQARLLVGLFNDGYIVEELSNNKLV 448
           V R  +     G   WG+W+   G  A P+S  NH QARLL+GLFNDGY VEE   NK+V
Sbjct: 463 VSRAYRGVDGEGRCGWGQWMRGSGFTAVPLSCFNHSQARLLLGLFNDGYTVEETGPNKIV 522

BLAST of MS015671 vs. ExPASy Swiss-Prot
Match: Q9SUF5 (Scarecrow-like protein 26 OS=Arabidopsis thaliana OX=3702 GN=SCL26 PE=2 SV=1)

HSP 1 Score: 411.4 bits (1056), Expect = 1.3e-113
Identity = 232/452 (51.33%), Postives = 297/452 (65.71%), Query Frame = 0

Query: 6   DADWEAFPGAGDFHDLIDS-------MMDADVPTYAAEVDGSSNCNSEEEESTEA----- 65
           D DW+      DF D+I+S       MM+ +        D    CNS     + A     
Sbjct: 37  DIDWDC-----DFRDVIESIMGDEGAMMEPESEAVPMLHDQEGLCNSASTGLSVADGVSF 96

Query: 66  -EDQEEELKGLRLYHLLMAAADALYGEHKSRDLAHVILLRLNELVSPSHGTNLERLAAYY 125
            E + +E KGLRL HLL+AAADA  G +KSR+L  VIL RL +LVSP   TN+ERLAA++
Sbjct: 97  GEPKTDESKGLRLVHLLVAAADASTGANKSRELTRVILARLKDLVSPGDRTNMERLAAHF 156

Query: 126 TQALQGLLECTAGVGKSHHHLHRDD-HTPGDVVAAFQLLQEMSPYVKFGHFTANQAILEA 185
           T  L  LLE  + +       HRDD +   DV++AF+LLQ MSPYV FG+ TA QAILEA
Sbjct: 157 TNGLSKLLERDSVLCPQQ---HRDDVYDQADVISAFELLQNMSPYVNFGYLTATQAILEA 216

Query: 186 VGDDRRVHIVDYDIMEGIQWASLMQAFVSRKDGPPAPHLRITAISRGGSTARRSIGTVQE 245
           V  +RR+HIVDYDI EG+QWASLMQA VSR  GP A HLRITA+SR  +  ++S+  VQE
Sbjct: 217 VKYERRIHIVDYDINEGVQWASLMQALVSRNTGPSAQHLRITALSR-ATNGKKSVAAVQE 276

Query: 246 TGRRLVAFAASIGQPFSFHQCKLESDESFRAAGLKLVKGEAVVVNCMLHLPHFRHRTPES 305
           TGRRL AFA SIGQPFS+  CKL+++ +F  + LKLV+GEAVV+NCMLHLP F H+TP S
Sbjct: 277 TGRRLTAFADSIGQPFSYQHCKLDTN-AFSTSSLKLVRGEAVVINCMLHLPRFSHQTPSS 336

Query: 306 IGSFLRGAEALSPRVVCLVEEEIG-HGIGDYKVEFVESLERYSAIYESLEVGLPMNNRGR 365
           + SFL  A+ L+P++V LV EE+G  G   +   F++ L ++SAI++SLE GL + N  R
Sbjct: 337 VISFLSEAKTLNPKLVTLVHEEVGLMGNQGFLYRFMDLLHQFSAIFDSLEAGLSIANPAR 396

Query: 366 ALVERVFLGPQIWGSVGRM---GQPRTAAGSWGEWLGKMGMKAAPISFANHCQARLLVGL 425
             VERVF+GP +   + R+        +  SW +WL   G K   +SF N CQA+LL+ L
Sbjct: 397 GFVERVFIGPWVANWLTRITANDAEVESFASWPQWLETNGFKPLEVSFTNRCQAKLLLSL 456

Query: 426 FNDGYIVEELSNNKLVLGWKSKRLLSASIWAS 440
           FNDG+ VEEL  N LVLGWKS+RL+SAS WAS
Sbjct: 457 FNDGFRVEELGQNGLVLGWKSRRLVSASFWAS 478

BLAST of MS015671 vs. ExPASy Swiss-Prot
Match: A0A0M4FMK2 (GRAS family protein RAM1 OS=Petunia hybrida OX=4102 GN=RAM1 PE=2 SV=1)

HSP 1 Score: 203.0 bits (515), Expect = 7.3e-51
Identity = 141/394 (35.79%), Postives = 206/394 (52.28%), Query Frame = 0

Query: 53  AEDQEEELKGLRLYHLLMAAADALYGEHKSRDLAHVILLRLNELVSPSHGTNLERLAAYY 112
           A DQE++  GL+L HLL+A A+A+  E     LA   L  LN +V+P  G +++R+A+ +
Sbjct: 319 APDQEQD-SGLQLVHLLLACAEAVSKEDYM--LARRYLHHLNRVVTPL-GDSMQRVASCF 378

Query: 113 TQALQGLLECTAGVGKSHHHLHRDDHTPG---DVVAAFQLLQEMSPYVKFGHFTANQAIL 172
           T+AL   L  T     S   L   +  P    +++  +Q+L +  PYVKF HFTANQAI 
Sbjct: 379 TEALSARLAATLATKPSTSALKPFNPFPPNSLEILKIYQILYQACPYVKFAHFTANQAIF 438

Query: 173 EAVGDDRRVHIVDYDIMEGIQWASLMQAFVSRKDGPPAPHLRITAISRGGSTARRSIGTV 232
           EA   + RVHI+D DI++G QW + MQA  +R  G  AP LRIT +         S   V
Sbjct: 439 EAFEAEERVHIIDLDILQGYQWPAFMQALAARPGG--APFLRITGVG-------PSPEAV 498

Query: 233 QETGRRLVAFAASIGQPFSFHQCKLESDESFRAAGLKLVKGEAVVVNCMLHLPHFRHRTP 292
           +ETGR L   A S+  PF FH    E  E  +        GEA+ VN +  L    HR P
Sbjct: 499 RETGRCLTELAHSLHVPFEFHPVG-EQLEDLKPHMFNRRVGEALAVNSVNRL----HRVP 558

Query: 293 -ESIGSFLRGAEALSPRVVCLVEEEIGHGIGDYKVEFVESLERYSAIYESLEVGLPMNNR 352
              IG+ L      +P +V +VE+E  H    +   F+E+L  YSAI++SL+   P ++ 
Sbjct: 559 VNCIGNLLAMIRDQAPNIVTIVEQEASHNGPYFLGRFLEALHYYSAIFDSLDATFPADSS 618

Query: 353 GRALVERVFLGPQIWGSVGRMGQPRTA----AGSWGEWLGKMGMKAAPISFANHCQARLL 412
            RA +E+    P+I   V   G  R         W   +   G K   +S     Q+++L
Sbjct: 619 QRAKLEQYIFAPEIMNIVSCEGPERVVRHERLEKWRRLMEGKGFKGVALSANAVTQSKIL 678

Query: 413 VGLFN-DGYIVEELSNNKLVLGWKSKRLLSASIW 438
           +GL++ DGY + E  +  L+LGW+ + +L+AS W
Sbjct: 679 LGLYSCDGYKLTE-DDGCLLLGWQDRAILAASAW 693

BLAST of MS015671 vs. ExPASy TrEMBL
Match: A0A6J1JUC4 (nodulation-signaling pathway 2 protein OS=Cucurbita maxima OX=3661 GN=LOC111489805 PE=3 SV=1)

HSP 1 Score: 631.7 bits (1628), Expect = 2.3e-177
Identity = 333/453 (73.51%), Postives = 376/453 (83.00%), Query Frame = 0

Query: 17  DFHDLIDSMMDADVPTYA---AEVDGSSNCNSEEEESTEAEDQEEELKGLRLYHLLMAAA 76
           DFHDL DS+M  D   Y+    EVDG +NCNS    + + E++EEE K LRLYHLLMA A
Sbjct: 27  DFHDLFDSIMSIDALPYSPQVVEVDGGTNCNSPSNPAEDREEEEEEFKSLRLYHLLMAVA 86

Query: 77  DALYGEHKSRDLAHVILLRLNELVSPSHGTNLERLAAYYTQALQGLLECTA---GVGKSH 136
           DA++G++KSR+LA VIL+RLNELVS SHGTNLERL AYY QA Q LL+C A   GV    
Sbjct: 87  DAMFGDNKSRELAQVILIRLNELVSTSHGTNLERLTAYYAQAFQDLLDCAAVSGGVPNKP 146

Query: 137 HHLHRDDHTPGDVVAAFQLLQEMSPYVKFGHFTANQAILEAVGDDRRVHIVDYDIMEGIQ 196
           HHLHRDDH+P DV+AAFQLLQ+MSPYVKFGHFTANQAILEAV DDRRVHIVDY+IMEGIQ
Sbjct: 147 HHLHRDDHSPTDVLAAFQLLQDMSPYVKFGHFTANQAILEAVADDRRVHIVDYNIMEGIQ 206

Query: 197 WASLMQAFVSRKDGPPAPHLRITAISRGGSTARRSIGTVQETGRRLVAFAASIGQPFSFH 256
           WASLMQAFVSRKD PPAPHLRIT ISRG S +RR IGTVQETGRRLVAFAASIGQPFSFH
Sbjct: 207 WASLMQAFVSRKDSPPAPHLRITVISRGPS-SRRWIGTVQETGRRLVAFAASIGQPFSFH 266

Query: 257 QCKLESDESFRAAGLKLVKGEAVVVNCMLHLPHFRHRTPESIGSFLRGAEALSPRVVCLV 316
           QCKL+SDESFR +GLKLV+GEA+VVNC+LHLPHF + +PESI SFL GA+ L+PR+V LV
Sbjct: 267 QCKLDSDESFRPSGLKLVRGEALVVNCVLHLPHFSNSSPESIASFLAGAKTLNPRLVTLV 326

Query: 317 EEEIGHG---IGDYKVEFVESLERYSAIYESLEVGLPMNNRGRALVERVFLGPQIWGSVG 376
           EEEI HG    GDYKV+F++SLERYSAIY+SLE G+PM NR R LVE+VFLGP+I  ++ 
Sbjct: 327 EEEIEHGPTIDGDYKVQFLDSLERYSAIYDSLEAGIPMKNRARGLVEKVFLGPRISATLR 386

Query: 377 RMGQPRTAAGS------WGEWLGKMGMKAAPISFANHCQARLLVGLFNDGYIVEELSNNK 436
           R+GQP+ AA +      WGE L KMG+KAA ISFANHCQARLLV LFNDGY VEEL +NK
Sbjct: 387 RIGQPQRAATAAEENCLWGERLEKMGLKAAAISFANHCQARLLVDLFNDGYRVEELGSNK 446

Query: 437 LVLGWKSKRLLSASIWASSSSPSSSSSSSSDSE 455
           LVLGWKSKRLLS SIWASSS  SSSSSSSSDSE
Sbjct: 447 LVLGWKSKRLLSVSIWASSS--SSSSSSSSDSE 476

BLAST of MS015671 vs. ExPASy TrEMBL
Match: A0A6J1FK48 (nodulation-signaling pathway 2 protein OS=Cucurbita moschata OX=3662 GN=LOC111444761 PE=3 SV=1)

HSP 1 Score: 622.9 bits (1605), Expect = 1.1e-174
Identity = 329/453 (72.63%), Postives = 372/453 (82.12%), Query Frame = 0

Query: 17  DFHDLIDSMMDADVPTYA---AEVDGSSNCNSEEEESTEAEDQEEELKGLRLYHLLMAAA 76
           DFHDL DS+M  D   Y+    E+DG +NCNS    +   E++EEE + LRLYHLLMA A
Sbjct: 27  DFHDLFDSIMSIDALPYSPQVVEIDGGTNCNSPSNPAEGREEEEEEFRSLRLYHLLMAVA 86

Query: 77  DALYGEHKSRDLAHVILLRLNELVSPSHGTNLERLAAYYTQALQGLLECTA---GVGKSH 136
           DA++G+HKSR+LA VIL+RLNELVS SHGTNLER+ AYY QA Q LL+C A   G     
Sbjct: 87  DAMFGDHKSRELAQVILIRLNELVSTSHGTNLERITAYYAQAFQDLLDCAAVSGGGPNKP 146

Query: 137 HHLHRDDHTPGDVVAAFQLLQEMSPYVKFGHFTANQAILEAVGDDRRVHIVDYDIMEGIQ 196
           HHLHRDDH+P DV+AAFQLLQEMSPYVKFGHFTANQAILEAV DDRRVHIVDY+IMEGIQ
Sbjct: 147 HHLHRDDHSPTDVLAAFQLLQEMSPYVKFGHFTANQAILEAVADDRRVHIVDYNIMEGIQ 206

Query: 197 WASLMQAFVSRKDGPPAPHLRITAISRGGSTARRSIGTVQETGRRLVAFAASIGQPFSFH 256
           WASLMQA VSRKD PPAPHLRITAISRG S +RR IGTVQETGRRLVAFAASIGQPFSFH
Sbjct: 207 WASLMQALVSRKDSPPAPHLRITAISRGPS-SRRWIGTVQETGRRLVAFAASIGQPFSFH 266

Query: 257 QCKLESDESFRAAGLKLVKGEAVVVNCMLHLPHFRHRTPESIGSFLRGAEALSPRVVCLV 316
           QCKL+SDESFR +GLKLV+GEA+VVNC+LHLPHF + +PESI SFL GA+ L+PR+V LV
Sbjct: 267 QCKLDSDESFRPSGLKLVRGEALVVNCVLHLPHFSNTSPESIASFLAGAKTLNPRLVTLV 326

Query: 317 EEEIGHG---IGDYKVEFVESLERYSAIYESLEVGLPMNNRGRALVERVFLGPQIWGSVG 376
           EEEI HG    GDYKV+F++SLERYSAIY+SLE G+PM NR R LVE+VFLGP+I  ++ 
Sbjct: 327 EEEIEHGPTIDGDYKVQFLDSLERYSAIYDSLEAGIPMKNRARGLVEKVFLGPRISATLR 386

Query: 377 RMGQPRTAAGS------WGEWLGKMGMKAAPISFANHCQARLLVGLFNDGYIVEELSNNK 436
           R+ QP+ AA +      WGE L KMG+KAA ISFANHCQARLLV LFNDGY VEEL +NK
Sbjct: 387 RIEQPQRAATAAEENCLWGERLEKMGLKAAAISFANHCQARLLVDLFNDGYRVEELGSNK 446

Query: 437 LVLGWKSKRLLSASIWASSSSPSSSSSSSSDSE 455
           LVLGWKSKRLLS SIWASSS   SSSSSSSDSE
Sbjct: 447 LVLGWKSKRLLSVSIWASSS--LSSSSSSSDSE 476

BLAST of MS015671 vs. ExPASy TrEMBL
Match: A0A5A7V767 (Nodulation-signaling pathway 2 protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1415G00580 PE=3 SV=1)

HSP 1 Score: 611.3 bits (1575), Expect = 3.3e-171
Identity = 330/468 (70.51%), Postives = 370/468 (79.06%), Query Frame = 0

Query: 1   DYCSPDADWEAFPGA-GDFHDLIDSMMDADVPTYAAEVDGSSNCNSEEEESTEAEDQEEE 60
           +Y SP  DWE F G   DF D+ DS +  + P +                 TE  D+E+E
Sbjct: 35  NYSSPIVDWETFSGTHNDFLDVFDSTICIENPPFL----------------TEDRDEEDE 94

Query: 61  LKGLRLYHLLMAAADALYGEHKSRDLAHVILLRLNELVSPSHGTNLERLAAYYTQALQGL 120
            KGLRLYHLL+AAADA++G+H+S DLAHVIL+RLNELVSPSHGTNLERL AYY QA Q L
Sbjct: 95  FKGLRLYHLLIAAADAVFGDHRSCDLAHVILVRLNELVSPSHGTNLERLTAYYAQAFQDL 154

Query: 121 LECTAGVGKSHHH----LHRDDHTPGDVVAAFQLLQEMSPYVKFGHFTANQAILEAVGDD 180
           L+      K+HHH     HRDDHTP DV+AAFQLLQEMSPYVKFGHFTANQAILEAV +D
Sbjct: 155 LDSAPVSNKTHHHNHHIHHRDDHTPTDVLAAFQLLQEMSPYVKFGHFTANQAILEAVAED 214

Query: 181 RRVHIVDYDIMEGIQWASLMQAFVSRKDGPPAPHLRITAISRGGSTARRSIGTVQETGRR 240
           RRVHIVDYDIMEGIQWASLMQAFVS    P APHLRITAISR G+  RRSIGTVQETGRR
Sbjct: 215 RRVHIVDYDIMEGIQWASLMQAFVS---SPSAPHLRITAISR-GANGRRSIGTVQETGRR 274

Query: 241 LVAFAASIGQPFSFHQCKLESDESFRAAGLKLVKGEAVVVNCMLHLPHFRHRTPESIGSF 300
           LVAFAASIGQPFSFHQCKL+SDESFR +GLKLVKGEA+VVNCMLHLPHF +R+PESI SF
Sbjct: 275 LVAFAASIGQPFSFHQCKLDSDESFRPSGLKLVKGEALVVNCMLHLPHFSYRSPESIASF 334

Query: 301 LRGAEALSPRVVCLVEEEIGHG---IGDYKVEFVESLERYSAIYESLEVGLPMNNRGRAL 360
           L GA++LSPR+V LVEEEIGHG    GDYKV+F++SLERYSAIY+SLE  +PM NR RAL
Sbjct: 335 LSGAKSLSPRIVTLVEEEIGHGPTIDGDYKVQFLDSLERYSAIYDSLEAVIPMKNRARAL 394

Query: 361 VERVFLGPQIWGSVGRMGQPRTAAGS------WGEWLGKMGMKAAPISFANHCQARLLVG 420
           VERVFLGP+I  ++ R+GQ R +  +      WGE L KMG+K   ISFANHCQARLL+G
Sbjct: 395 VERVFLGPRISATLRRIGQRRWSTAAMEENCLWGEKLEKMGLKTVKISFANHCQARLLLG 454

Query: 421 LFNDGYIVEELSNNKLVLGWKSKRLLSASIWASSSSPSSSSSSSSDSE 455
           LFNDGY VEEL NNKLVLGWKSKRLLS SIW SS+S SSSSSS SDSE
Sbjct: 455 LFNDGYRVEELGNNKLVLGWKSKRLLSVSIWGSSTS-SSSSSSLSDSE 481

BLAST of MS015671 vs. ExPASy TrEMBL
Match: A0A1S3BE54 (nodulation-signaling pathway 2 protein OS=Cucumis melo OX=3656 GN=LOC103488636 PE=3 SV=1)

HSP 1 Score: 611.3 bits (1575), Expect = 3.3e-171
Identity = 330/468 (70.51%), Postives = 370/468 (79.06%), Query Frame = 0

Query: 1   DYCSPDADWEAFPGA-GDFHDLIDSMMDADVPTYAAEVDGSSNCNSEEEESTEAEDQEEE 60
           +Y SP  DWE F G   DF D+ DS +  + P +                 TE  D+E+E
Sbjct: 35  NYSSPIVDWETFSGTHNDFLDVFDSTICIENPPFL----------------TEDRDEEDE 94

Query: 61  LKGLRLYHLLMAAADALYGEHKSRDLAHVILLRLNELVSPSHGTNLERLAAYYTQALQGL 120
            KGLRLYHLL+AAADA++G+H+S DLAHVIL+RLNELVSPSHGTNLERL AYY QA Q L
Sbjct: 95  FKGLRLYHLLIAAADAVFGDHRSCDLAHVILVRLNELVSPSHGTNLERLTAYYAQAFQDL 154

Query: 121 LECTAGVGKSHHH----LHRDDHTPGDVVAAFQLLQEMSPYVKFGHFTANQAILEAVGDD 180
           L+      K+HHH     HRDDHTP DV+AAFQLLQEMSPYVKFGHFTANQAILEAV +D
Sbjct: 155 LDSAPVSNKTHHHNHHIHHRDDHTPTDVLAAFQLLQEMSPYVKFGHFTANQAILEAVAED 214

Query: 181 RRVHIVDYDIMEGIQWASLMQAFVSRKDGPPAPHLRITAISRGGSTARRSIGTVQETGRR 240
           RRVHIVDYDIMEGIQWASLMQAFVS    P APHLRITAISR G+  RRSIGTVQETGRR
Sbjct: 215 RRVHIVDYDIMEGIQWASLMQAFVS---SPSAPHLRITAISR-GANGRRSIGTVQETGRR 274

Query: 241 LVAFAASIGQPFSFHQCKLESDESFRAAGLKLVKGEAVVVNCMLHLPHFRHRTPESIGSF 300
           LVAFAASIGQPFSFHQCKL+SDESFR +GLKLVKGEA+VVNCMLHLPHF +R+PESI SF
Sbjct: 275 LVAFAASIGQPFSFHQCKLDSDESFRPSGLKLVKGEALVVNCMLHLPHFSYRSPESIASF 334

Query: 301 LRGAEALSPRVVCLVEEEIGHG---IGDYKVEFVESLERYSAIYESLEVGLPMNNRGRAL 360
           L GA++LSPR+V LVEEEIGHG    GDYKV+F++SLERYSAIY+SLE  +PM NR RAL
Sbjct: 335 LSGAKSLSPRIVTLVEEEIGHGPTIDGDYKVQFLDSLERYSAIYDSLEAVIPMKNRARAL 394

Query: 361 VERVFLGPQIWGSVGRMGQPRTAAGS------WGEWLGKMGMKAAPISFANHCQARLLVG 420
           VERVFLGP+I  ++ R+GQ R +  +      WGE L KMG+K   ISFANHCQARLL+G
Sbjct: 395 VERVFLGPRISATLRRIGQRRWSTAAMEENCLWGEKLEKMGLKTVKISFANHCQARLLLG 454

Query: 421 LFNDGYIVEELSNNKLVLGWKSKRLLSASIWASSSSPSSSSSSSSDSE 455
           LFNDGY VEEL NNKLVLGWKSKRLLS SIW SS+S SSSSSS SDSE
Sbjct: 455 LFNDGYRVEELGNNKLVLGWKSKRLLSVSIWGSSTS-SSSSSSLSDSE 481

BLAST of MS015671 vs. ExPASy TrEMBL
Match: A0A0A0KDX4 (GRAS domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G040600 PE=3 SV=1)

HSP 1 Score: 607.1 bits (1564), Expect = 6.2e-170
Identity = 329/468 (70.30%), Postives = 364/468 (77.78%), Query Frame = 0

Query: 1   DYCSPDADWEAFPGA-GDFHDLIDSMMDADVPTYAAEVDGSSNCNSEEEESTEAEDQEEE 60
           +Y SP  DWE F G   DF D+ DS +  + P +                 TE  D+E+E
Sbjct: 35  NYGSPIVDWETFSGTHNDFLDVFDSTICIENPPFL----------------TEDRDEEDE 94

Query: 61  LKGLRLYHLLMAAADALYGEHKSRDLAHVILLRLNELVSPSHGTNLERLAAYYTQALQGL 120
            KGLRLYHLL AAADA+ G+HKS DLAHVIL+RLNELVSPSHGTNLERL AYY QA Q L
Sbjct: 95  FKGLRLYHLLTAAADAVLGDHKSCDLAHVILVRLNELVSPSHGTNLERLTAYYAQAFQDL 154

Query: 121 LECTAGVGKSHHHLH----RDDHTPGDVVAAFQLLQEMSPYVKFGHFTANQAILEAVGDD 180
           L+      K+HHH H    RDDHTP DV+AAFQLLQEMSPYVKFGHFTANQAILEAV +D
Sbjct: 155 LDSAPVANKTHHHNHHIHQRDDHTPTDVLAAFQLLQEMSPYVKFGHFTANQAILEAVAED 214

Query: 181 RRVHIVDYDIMEGIQWASLMQAFVSRKDGPPAPHLRITAISRGGSTARRSIGTVQETGRR 240
           RRVHIVDYDIMEGIQWASLMQAFVS    P APHLRITAISR G+  RRSIGTVQETGRR
Sbjct: 215 RRVHIVDYDIMEGIQWASLMQAFVS---SPSAPHLRITAISR-GANGRRSIGTVQETGRR 274

Query: 241 LVAFAASIGQPFSFHQCKLESDESFRAAGLKLVKGEAVVVNCMLHLPHFRHRTPESIGSF 300
           LVAFAASIGQPFSFHQCKL+SDESFR +GLKLVKGEA+VVNCMLHLPHF +R+PESI SF
Sbjct: 275 LVAFAASIGQPFSFHQCKLDSDESFRPSGLKLVKGEALVVNCMLHLPHFSYRSPESIASF 334

Query: 301 LRGAEALSPRVVCLVEEEIGHG---IGDYKVEFVESLERYSAIYESLEVGLPMNNRGRAL 360
           L GA++LSPR+V LVEEEIGHG    GDYKV+F++SLERYSAIY+SLE  +PM NR RAL
Sbjct: 335 LSGAKSLSPRIVTLVEEEIGHGPTIDGDYKVQFLDSLERYSAIYDSLEAVIPMKNRARAL 394

Query: 361 VERVFLGPQIWGSVGRMGQPRTAAGS------WGEWLGKMGMKAAPISFANHCQARLLVG 420
           VERVFLGP+I  ++ R+GQ R +         WGE L KMG+K   ISFANHCQARLL+G
Sbjct: 395 VERVFLGPRISATLRRIGQRRWSTAEMEDNCLWGEKLEKMGLKTVKISFANHCQARLLLG 454

Query: 421 LFNDGYIVEELSNNKLVLGWKSKRLLSASIWASSSSPSSSSSSSSDSE 455
           LFNDGY VEEL NNKLVLGWKSKRLLS SIW S    SSSSSS SDSE
Sbjct: 455 LFNDGYRVEELGNNKLVLGWKSKRLLSVSIWGS----SSSSSSLSDSE 478

BLAST of MS015671 vs. TAIR 10
Match: AT4G08250.1 (GRAS family transcription factor )

HSP 1 Score: 411.4 bits (1056), Expect = 9.6e-115
Identity = 232/452 (51.33%), Postives = 297/452 (65.71%), Query Frame = 0

Query: 6   DADWEAFPGAGDFHDLIDS-------MMDADVPTYAAEVDGSSNCNSEEEESTEA----- 65
           D DW+      DF D+I+S       MM+ +        D    CNS     + A     
Sbjct: 37  DIDWDC-----DFRDVIESIMGDEGAMMEPESEAVPMLHDQEGLCNSASTGLSVADGVSF 96

Query: 66  -EDQEEELKGLRLYHLLMAAADALYGEHKSRDLAHVILLRLNELVSPSHGTNLERLAAYY 125
            E + +E KGLRL HLL+AAADA  G +KSR+L  VIL RL +LVSP   TN+ERLAA++
Sbjct: 97  GEPKTDESKGLRLVHLLVAAADASTGANKSRELTRVILARLKDLVSPGDRTNMERLAAHF 156

Query: 126 TQALQGLLECTAGVGKSHHHLHRDD-HTPGDVVAAFQLLQEMSPYVKFGHFTANQAILEA 185
           T  L  LLE  + +       HRDD +   DV++AF+LLQ MSPYV FG+ TA QAILEA
Sbjct: 157 TNGLSKLLERDSVLCPQQ---HRDDVYDQADVISAFELLQNMSPYVNFGYLTATQAILEA 216

Query: 186 VGDDRRVHIVDYDIMEGIQWASLMQAFVSRKDGPPAPHLRITAISRGGSTARRSIGTVQE 245
           V  +RR+HIVDYDI EG+QWASLMQA VSR  GP A HLRITA+SR  +  ++S+  VQE
Sbjct: 217 VKYERRIHIVDYDINEGVQWASLMQALVSRNTGPSAQHLRITALSR-ATNGKKSVAAVQE 276

Query: 246 TGRRLVAFAASIGQPFSFHQCKLESDESFRAAGLKLVKGEAVVVNCMLHLPHFRHRTPES 305
           TGRRL AFA SIGQPFS+  CKL+++ +F  + LKLV+GEAVV+NCMLHLP F H+TP S
Sbjct: 277 TGRRLTAFADSIGQPFSYQHCKLDTN-AFSTSSLKLVRGEAVVINCMLHLPRFSHQTPSS 336

Query: 306 IGSFLRGAEALSPRVVCLVEEEIG-HGIGDYKVEFVESLERYSAIYESLEVGLPMNNRGR 365
           + SFL  A+ L+P++V LV EE+G  G   +   F++ L ++SAI++SLE GL + N  R
Sbjct: 337 VISFLSEAKTLNPKLVTLVHEEVGLMGNQGFLYRFMDLLHQFSAIFDSLEAGLSIANPAR 396

Query: 366 ALVERVFLGPQIWGSVGRM---GQPRTAAGSWGEWLGKMGMKAAPISFANHCQARLLVGL 425
             VERVF+GP +   + R+        +  SW +WL   G K   +SF N CQA+LL+ L
Sbjct: 397 GFVERVFIGPWVANWLTRITANDAEVESFASWPQWLETNGFKPLEVSFTNRCQAKLLLSL 456

Query: 426 FNDGYIVEELSNNKLVLGWKSKRLLSASIWAS 440
           FNDG+ VEEL  N LVLGWKS+RL+SAS WAS
Sbjct: 457 FNDGFRVEELGQNGLVLGWKSRRLVSASFWAS 478

BLAST of MS015671 vs. TAIR 10
Match: AT3G54220.1 (GRAS family transcription factor )

HSP 1 Score: 191.8 bits (486), Expect = 1.2e-48
Identity = 137/400 (34.25%), Postives = 211/400 (52.75%), Query Frame = 0

Query: 48  EESTEAEDQEEELKGLRLYHLLMAAADALYGEHKSRDLAHVILLRLNELVSPSHGTNLER 107
           E   E + Q+++ +GL L  LL+  A+A+  ++     A+ +LL +++L +P +GT+ +R
Sbjct: 273 ERKEEIKRQKQDEEGLHLLTLLLQCAEAVSADNLEE--ANKLLLEISQLSTP-YGTSAQR 332

Query: 108 LAAYYTQALQG-LLECTAGVGKSHHHLHRDDHTPGDVVAAFQLLQEMSPYVKFGHFTANQ 167
           +AAY+++A+   LL    G+  +             +V+AFQ+   +SP VKF HFTANQ
Sbjct: 333 VAAYFSEAMSARLLNSCLGIYAALPSRWMPQTHSLKMVSAFQVFNGISPLVKFSHFTANQ 392

Query: 168 AILEAVGDDRRVHIVDYDIMEGIQWASLMQAFVSRKDGPPAPHLRITAISRGGSTARRSI 227
           AI EA   +  VHI+D DIM+G+QW  L     SR  GP  PH+R+T +         S+
Sbjct: 393 AIQEAFEKEDSVHIIDLDIMQGLQWPGLFHILASRPGGP--PHVRLTGLG-------TSM 452

Query: 228 GTVQETGRRLVAFAASIGQPFSFHQCKL-ESDESFRAAGLKLVKGEAVVVNCMLHLPHFR 287
             +Q TG+RL  FA  +G PF F  C L E   +     L + K EAV V+    L H  
Sbjct: 453 EALQATGKRLSDFADKLGLPFEF--CPLAEKVGNLDTERLNVRKREAVAVH---WLQHSL 512

Query: 288 HRTPESIGSFLRGAEALSPRVVCLVEEEIGHGIGDYKVEFVESLERYSAIYESLEVGLPM 347
           +    S    L   + L+P+VV +VE+++ H  G +   FVE++  YSA+++SL      
Sbjct: 513 YDVTGSDAHTLWLLQRLAPKVVTVVEQDLSHA-GSFLGRFVEAIHYYSALFDSLGASYGE 572

Query: 348 NNRGRALVERVFLGPQIWGSVGRMGQPRTAA---GSWGEWLGKMGMKAAPISFANHCQAR 407
            +  R +VE+  L  +I   +   G  R+      SW E + + G K   ++     QA 
Sbjct: 573 ESEERHVVEQQLLSKEIRNVLAVGGPSRSGEVKFESWREKMQQCGFKGISLAGNAATQAT 632

Query: 408 LLVGLF-NDGYIVEELSNNKLVLGWKSKRLLSASIWASSS 442
           LL+G+F +DGY + +  N  L LGWK   LL+AS W   S
Sbjct: 633 LLLGMFPSDGYTLVD-DNGTLKLGWKDLSLLTASAWTPRS 653

BLAST of MS015671 vs. TAIR 10
Match: AT1G55580.1 (GRAS family transcription factor )

HSP 1 Score: 177.6 bits (449), Expect = 2.3e-44
Identity = 137/447 (30.65%), Postives = 203/447 (45.41%), Query Frame = 0

Query: 40  SSNCNSEEEESTEAED------QEEELKGLRLYHLLMAAADALYGEHKSRDLAHVILLRL 99
           SS+ +SE+  +T  E+                +HL      A     +S   A   LL +
Sbjct: 7   SSSSSSEDATATTTENPPPLCIASSSAATSASHHLRRLLFTAANFVSQSNFTAAQNLLSI 66

Query: 100 NELVSPSHGTNLERLAAYYTQALQGLLE--------------------------CTAGVG 159
             L S  HG + ERL   +T+AL   +                            T+ V 
Sbjct: 67  LSLNSSPHGDSTERLVHLFTKALSVRINRQQQDQTAETVATWTTNEMTMSNSTVFTSSVC 126

Query: 160 KSHHHLHRDDHTPGDVVAAFQL-LQEMSPYVKFGHFTANQAILEA--VGDDRRVHIVDYD 219
           K    L R  +   D  + + L L +++P+++FGH TANQAIL+A    D+  +HI+D D
Sbjct: 127 K-EQFLFRTKNNNSDFESCYYLWLNQLTPFIRFGHLTANQAILDATETNDNGALHILDLD 186

Query: 220 IMEGIQWASLMQAFVSRKDGP--PAPHLRITAISRGGSTARRSIGTVQETGRRLVAFAAS 279
           I +G+QW  LMQA   R   P  P P LRIT          R +  +  TG RL  FA S
Sbjct: 187 ISQGLQWPPLMQALAERSSNPSSPPPSLRITGCG-------RDVTGLNRTGDRLTRFADS 246

Query: 280 IGQPFSFHQCKLESDE------SFRAAGLKLVKGEAVVVNCMLHLPHFRHRTPESIGSFL 339
           +G  F FH   +  ++        R   L  V+GE + VNC+  L    +   + IG FL
Sbjct: 247 LGLQFQFHTLVIVEEDLAGLLLQIRLLALSAVQGETIAVNCVHFLHKIFNDDGDMIGHFL 306

Query: 340 RGAEALSPRVVCLVEEEIGHGIGDYKVEFVESLERYSAIYESLEVGLPMNNRGRALVERV 399
              ++L+ R+V + E E  HG   +   F E+++ Y AI++SLE  LP N+R R  +E+ 
Sbjct: 307 SAIKSLNSRIVTMAEREANHGDHSFLNRFSEAVDHYMAIFDSLEATLPPNSRERLTLEQR 366

Query: 400 FLGPQIWGSVG----RMGQPRTAAGSWGEWLGKMGMKAAPISFANHCQARLLVGLF--ND 438
           + G +I   V        Q       W E + + G    PI      QA+LL+ L   ++
Sbjct: 367 WFGKEILDVVAAEETERKQRHRRFEIWEEMMKRFGFVNVPIGSFALSQAKLLLRLHYPSE 426

BLAST of MS015671 vs. TAIR 10
Match: AT3G03450.1 (RGA-like 2 )

HSP 1 Score: 173.3 bits (438), Expect = 4.4e-43
Identity = 130/411 (31.63%), Postives = 204/411 (49.64%), Query Frame = 0

Query: 43  CNSEEEESTEAEDQEEELKGLRLYHLLMAAADALYGEHKSRDLAHVILLRLNELVSPSHG 102
           C S +E +      + +  G+RL H L+A A+A++ E  + +LA  ++ R+  L     G
Sbjct: 158 CESSDESTRSVVLVDSQETGVRLVHALVACAEAIHQE--NLNLADALVKRVGTLAGSQAG 217

Query: 103 TNLERLAAYYTQALQGLLECTAGVGKSHHHLHRDDHTPGDVVAA----FQLLQEM----- 162
             + ++A Y+ QAL                ++RD     DV AA    F+ + EM     
Sbjct: 218 A-MGKVATYFAQALA-------------RRIYRDYTAETDVCAAVNPSFEEVLEMHFYES 277

Query: 163 SPYVKFGHFTANQAILEAVGDDRRVHIVDYDIMEGIQWASLMQAFVSRKDGPPAPHLRIT 222
            PY+KF HFTANQAILEAV   RRVH++D  + +G+QW +LMQA   R  GPP+   R+T
Sbjct: 278 CPYLKFAHFTANQAILEAVTTARRVHVIDLGLNQGMQWPALMQALALRPGGPPS--FRLT 337

Query: 223 AISRGGSTARRSIGTVQETGRRLVAFAASIGQPFSFHQCKLESDESFRAAGLKL-VKGEA 282
            I   G     +  ++Q+ G +L  FA ++G  F F     ES         +   + E 
Sbjct: 338 GI---GPPQTENSDSLQQLGWKLAQFAQNMGVEFEFKGLAAESLSDLEPEMFETRPESET 397

Query: 283 VVVNCMLHLPHFRHRTPESIGSFLRGAEALSPRVVCLVEEEIGHGIGDYKVEFVESLERY 342
           +VVN +  L     R+  SI   L   +A+ P +V +VE+E  H    +   F E+L  Y
Sbjct: 398 LVVNSVFELHRLLARS-GSIEKLLNTVKAIKPSIVTVVEQEANHNGIVFLDRFNEALHYY 457

Query: 343 SAIYESLEVGLPMNNRGRALVERVFLGPQIWGSVGRMGQPRT----AAGSWGEWLGKMGM 402
           S++++SLE    + ++ R + E V+LG QI   V   G  R      A  W   +   G 
Sbjct: 458 SSLFDSLEDSYSLPSQDRVMSE-VYLGRQILNVVAAEGSDRVERHETAAQWRIRMKSAGF 517

Query: 403 KAAPISFANHCQARLLVGLF--NDGYIVEELSNNKLVLGWKSKRLLSASIW 438
               +  +   QA +L+ L+   DGY VEE ++  L++GW+++ L++ S W
Sbjct: 518 DPIHLGSSAFKQASMLLSLYATGDGYRVEE-NDGCLMIGWQTRPLITTSAW 544

BLAST of MS015671 vs. TAIR 10
Match: AT5G48150.1 (GRAS family transcription factor )

HSP 1 Score: 169.5 bits (428), Expect = 6.3e-42
Identity = 116/362 (32.04%), Postives = 186/362 (51.38%), Query Frame = 0

Query: 85  LAHVILLRLNELVSPSHGTNLERLAAYYTQALQGLLECTAGVGKS-HHHLHR-DDHTPGD 144
           +AH ++ +L ++VS S G  ++RL AY    L+GL+   A  G S +  L+R  +    +
Sbjct: 137 MAHSMMEKLRQMVSVS-GEPIQRLGAY---LLEGLVAQLASSGSSIYKALNRCPEPASTE 196

Query: 145 VVAAFQLLQEMSPYVKFGHFTANQAILEAVGDDRRVHIVDYDIMEGIQWASLMQAFVSRK 204
           +++   +L E+ PY KFG+ +AN AI EA+ ++ RVHI+D+ I +G QW +L+QAF +R 
Sbjct: 197 LLSYMHILYEVCPYFKFGYMSANGAIAEAMKEENRVHIIDFQIGQGSQWVTLIQAFAARP 256

Query: 205 DGPPAPHLRITAISRGGSTARRSIGTVQETGRRLVAFAASIGQPFSFHQCKLESDESFRA 264
            GP  P +RIT I    S   R  G +   G RL   A     PF F+   +   E  + 
Sbjct: 257 GGP--PRIRITGIDDMTSAYARG-GGLSIVGNRLAKLAKQFNVPFEFNSVSVSVSE-VKP 316

Query: 265 AGLKLVKGEAVVVN---CMLHLPHFRHRTPESIGSFLRGAEALSPRVVCLVEEEIGHGIG 324
             L +  GEA+ VN    + H+P     T       LR  ++LSP+VV LVE+E      
Sbjct: 317 KNLGVRPGEALAVNFAFVLHHMPDESVSTENHRDRLLRMVKSLSPKVVTLVEQESNTNTA 376

Query: 325 DYKVEFVESLERYSAIYESLEVGLPMNNRGRALVERVFLGPQIWGSVGRMGQPRT----A 384
            +   F+E++  Y+A++ES++V LP +++ R  VE+  L   +   +   G  R      
Sbjct: 377 AFFPRFMETMNYYAAMFESIDVTLPRDHKQRINVEQHCLARDVVNIIACEGADRVERHEL 436

Query: 385 AGSWGEWLGKMGMKAAPISFANHCQARLLVGLFNDGYIVEELSNNKLVLGWKSKRLLSAS 438
            G W    G  G    P+S   +   + L+  ++D Y +EE  +  L LGW  + L+++ 
Sbjct: 437 LGKWRSRFGMAGFTPYPLSPLVNSTIKSLLRNYSDKYRLEE-RDGALYLGWMHRDLVASC 489