MS015520 (gene) Bitter gourd (TR) v1

Overview


Name	MS015520
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Ribonuclease
Location	scaffold300: 236316 .. 240122 (-)
RNA-Seq Expression	MS015520
Synteny	MS015520

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCTTCGCAAGAGCCACTCCCCAAATGGGCTTCCAAGCCTTGTGTAATGGGCATCGATGAAGCTGGTCGAGGCCCTGTTTTAGGTTTTCTATTTTTCCTATTCGCTTCATTCCATTTCTGTATCTTTAATTTCGCTTCGATCATCATATTTTGGGATGCGAATTTGAGAACCCCTTGAAATCTTACTGCATTTGATCTCAGGGCCAATGGTGTATGGATGCCTGTACTGCGCTAGTTCCTATTTGGAAGAACTTTCGTCTTTGAGTTTTGCAGGTTTAGCTCGTCACCGAAATTTATTCTCTTATTTCGTTTACTTTTCTTCCAACATTTGAAGCTTTGGGTGCAGACTCAAAAACGCTGAAAGAAGAGAAGAGGGAGGAATTGTTTGAAAATTTGAAGGCTAATGAATCAATTGGATGGGCAGTTGATGTCATAGATCCACGGGAGCTATCATCCAAAATGTTAAATAAGTCAGTAATCGGTTGTTTTTAAATTTTTTCATTTGCCTACGTACTCTAACTTCCACTTTACATAATGAATTCGATGGTATAGAAAATGGAACTTAGCTGAAATCTCGTCATTCTTGACAAATGGTATTAAATGACCTCTTCTCAAGAGCTTAAGTTGATTTTGTGTGAACTTTATGTATATTTATATATTCTTAACACTCTCCCTTGCTTATGGACTCAGAATACATGAATCTAGATGTTAGTCCGAGTAGAACACATGAATTATTATATGAAAATGAAAGAGTGGGAAGATAATATTTCAATGATTTTAACTCACAACTACCTAGACCCAAAGACTTAGATATTAGTTCCTGTATTGATCCTATATTAAATTACTACTAGATTAAAGAACTTAAATTGATTCGTCAGGGTAAATTTATATTTTTAGTATCTTTCTTTAACAAATAGAAGACAAGATAAGAAAACCTATCTTCATCTAAAGTTAGGAAAGGGTCAGCAAAGGACTGGAACATCGAACATGGCTACATATGCTTTCCCTCAACCTTCCATTTTTGTTAATTTATGCACATCAAAGGGTGACTTTTGGTTTGAAGGGCTAGTTTCCCCCCGCTTACTTTATTAAGAAATATTACTTGTTTAAGTCGAGTCCGTGAAGTAAGATGCTAACATATTGTATTTTTGGGTTTTAGTTTCTAAAATATGTCTTTATTGGTCAACAGAGTTAAGATCAACCTAAATGAAATATCGCATGACTCTGCAATGGGTCTTGTCAGCAAGGTTCTAGGCATGGGAGTTCTTTTAACTGAGGTAAATCTCTTCCCGGTTACTTGCACTCATTAGTCGGTTATGTGATTGTAATATTGTAATAGCATAGCTATAAGTTCTTCATGGTGAGGAAGTCCAATGAATGGCCTTTGGTAAATTCCATGTATCTTACGATCATGATTTTATTTGTACCATCTGAAGAAGAGAGAGAATGAGGAATCTTGAACTATACATGTTATCATTTCATGTTTTTCTTTTTGCAAAAGACAAACAAAATCAAGCTATCGGTTATTTTCTATTCATTTGAATGCCTTTTTCTTGTTAATTAAAGATCTCCAAAGTTAACAAGAAAATAAATGAACTACTTTTCTTTGTTGATTACAATAGTAGGGTGTTAGACATTCTTCACAAAGAGGAAGATCTTTAAAAAGTGAATTTGGTTGTGTTTTCATGATTTATTCAGGTCATTAGTCAATAATATCAGAGAGGGAAAAAAAATCCTCATTAAGCATACCATATCTTTTATTGCCTTCATAGTTTTCTTGTTTATCCATTGATGATCTGAAGTCATCCGCAGATTTGTTCTACTCATTCCTTTTCAGATTTTATGCATTCATTCTTACCTTAGGGTGCCCCATGAACAATGTTAGATTAATCACTTGCATTTATTTTCAGGTTTATGTGGATACTGTTGGAGACCCTGAAAAGTACAGAATAAAATTATCTGAAAGATTTCCCAACGTTAAATTTGTCGTGGCAAAGAAGGCTGATAGTTTATATCCTGTTGTCAGTGGGGCAAGCATAGTCGCAAAGGTAATGCTAAAACTTTCTTTCTAGATCTGGCACTCCAAACTGTTTCCCACACTCAAATTCATCATCTCGACTTGTAGGTCACAAGAGATCGTGCGTTGCGGGAATGGGCATTAGAAGAAACAGCTGAAAACATGCATAGAAATTTTGGTTCTGGATACCCTGGAGGTGAATTTCACCTGCTATATGTTATTTGCAGCATAAAATCTCACGTTTTCACTGCACAAGGTGAAATCAATAATGCTCTTATGATTTAATCTATATAGATCCAACAACTAAATCTTGGTTAGAAGATCACAGACATTCGGTATTTGGGTTCCCCACATTGGTCCGCTTCAGTTGGGGTACATGCAATCCATATTTCAAAGACATTGTTGAAGTATCATGGTTAGTTGTTACTCAGCTCATCCAGCCATTTACATTTTCTTTATGCTCTCCATGGTACATCAAGGACAGGCGTTTTTTGCTATAATCTGCATGCTTCATGGTACCATTTTTTTATGATGGACGAGTCACATAATTTTCATAAATTTCCACATGAATCGAACTGTCAGTATTTTAGTTAAAAAATCAGACTATTATGTTTTTAAGACTTGTTCAAGTGCACTTGTCCAGAATATTATTAGTTGTCACAACGTATCAATCCTTATACTAACTATGTGGTTTTAAGTGCTTTGGTTGAATATGAAATTTGGTAATTCATAATAGACATTTTTTTATTGGTGTAATTCGCTTTACTGGGGTGTGCAGTTCTTTTCCATTCTGGATTTAAACCTGTTTTGTACTCAATGAGTTGTACCTTTATTCTAGTCCATTGTTTGGTTAAGTGCATCTGCTTTAGAGTTTGGACTTCCAAATAGTTTTGGTTCCTTCGCGGTGTCTACTATGTTGAAGCACATCTATCTTTTTTGTTTTTGTTTTCCTTTTTTGATCTCAGAGCCAGTTTATATGCATATTTTTTACTCATCTTACGAGACAATTTCTTGACACTACAATATTTGGTTGTCACGAAACTCATAAAATTTTAATGTCTTAAGTGGCCACTGTGAAGTTTAAACCCATTTTCCCGAAGCCACATTTACTTTTTTTTCTTTTGCAAGTTTCAATTCCACTAGACCAAACGAGTGTAGTTACCCCATCTTAGCTTTCAGTAAATGAACATATTGCATTATGTGATCCATGTGTTTACCTTTGAGCTCATATATTGAAAAATAGATGTCAAAACTTCTCATATTTAGAACCATCATGGGTGGTTTATTGGTTCAAAGGAGCTAGAATTAATTAAGAGGGAATGACTTCAAGCCCCATGATGGCCACCTACTTTAGGAATTAGTAATTCCTAAATGTTTTCTAGTACCAAATGTTGTAGGATCAGGTGGTTGGCCCGTGAGATTAGTCGAGGTGCGCATAAGCTGGCCCGGACACTCACGGATATCAAAAAAAAAAAAAACTTCTCATACTTCAACTTCCCTAACACTCACTCACAAACTTCAAATTGATTTTGTTACTAAGATTCTCTTGATTACCTCATCACAATGATGTCATACCATATCTTGTTTTCCTTTTTTTGTGCTTGGATTCACAGGGAATCAGACAAAGTGGATGAAGATGGGGCAAATGCTGGAAGTGGTAAGAAACAATTAAGACTAAGCAATGTTGGTATCACCACATCCAAGAGAAAGAGTGAAGAAATTGAATCAAATGCCAAAGGACGCTGCAAGTTTTTCCAGGCTAGAAAACTCGAACAACTCACTCACTTT

mRNA sequence

ATGTCTTCGCAAGAGCCACTCCCCAAATGGGCTTCCAAGCCTTGTGTAATGGGCATCGATGAAGCTGGTCGAGGCCCTGTTTTAGGGCCAATGGTGTATGGATGCCTGTACTGCGCTAGTTCCTATTTGGAAGAACTTTCGTCTTTGAGTTTTGCAGACTCAAAAACGCTGAAAGAAGAGAAGAGGGAGGAATTGTTTGAAAATTTGAAGGCTAATGAATCAATTGGATGGGCAGTTGATGTCATAGATCCACGGGAGCTATCATCCAAAATGTTAAATAAAGTTAAGATCAACCTAAATGAAATATCGCATGACTCTGCAATGGGTCTTGTCAGCAAGGTTCTAGGCATGGGAGTTCTTTTAACTGAGGTTTATGTGGATACTGTTGGAGACCCTGAAAAGTACAGAATAAAATTATCTGAAAGATTTCCCAACGTTAAATTTGTCGTGGCAAAGAAGGCTGATAGTTTATATCCTGTTGTCAGTGGGGCAAGCATAGTCGCAAAGGTCACAAGAGATCGTGCGTTGCGGGAATGGGCATTAGAAGAAACAGCTGAAAACATGCATAGAAATTTTGGTTCTGGATACCCTGGAGATCCAACAACTAAATCTTGGTTAGAAGATCACAGACATTCGGTATTTGGGTTCCCCACATTGGTCCGCTTCAGTTGGGGTACATGCAATCCATATTTCAAAGACATTGTTGAAGTATCATGGGAATCAGACAAAGTGGATGAAGATGGGGCAAATGCTGGAAGTGGTAAGAAACAATTAAGACTAAGCAATGTTGGTATCACCACATCCAAGAGAAAGAGTGAAGAAATTGAATCAAATGCCAAAGGACGCTGCAAGTTTTTCCAGGCTAGAAAACTCGAACAACTCACTCACTTT

Coding sequence (CDS)

ATGTCTTCGCAAGAGCCACTCCCCAAATGGGCTTCCAAGCCTTGTGTAATGGGCATCGATGAAGCTGGTCGAGGCCCTGTTTTAGGGCCAATGGTGTATGGATGCCTGTACTGCGCTAGTTCCTATTTGGAAGAACTTTCGTCTTTGAGTTTTGCAGACTCAAAAACGCTGAAAGAAGAGAAGAGGGAGGAATTGTTTGAAAATTTGAAGGCTAATGAATCAATTGGATGGGCAGTTGATGTCATAGATCCACGGGAGCTATCATCCAAAATGTTAAATAAAGTTAAGATCAACCTAAATGAAATATCGCATGACTCTGCAATGGGTCTTGTCAGCAAGGTTCTAGGCATGGGAGTTCTTTTAACTGAGGTTTATGTGGATACTGTTGGAGACCCTGAAAAGTACAGAATAAAATTATCTGAAAGATTTCCCAACGTTAAATTTGTCGTGGCAAAGAAGGCTGATAGTTTATATCCTGTTGTCAGTGGGGCAAGCATAGTCGCAAAGGTCACAAGAGATCGTGCGTTGCGGGAATGGGCATTAGAAGAAACAGCTGAAAACATGCATAGAAATTTTGGTTCTGGATACCCTGGAGATCCAACAACTAAATCTTGGTTAGAAGATCACAGACATTCGGTATTTGGGTTCCCCACATTGGTCCGCTTCAGTTGGGGTACATGCAATCCATATTTCAAAGACATTGTTGAAGTATCATGGGAATCAGACAAAGTGGATGAAGATGGGGCAAATGCTGGAAGTGGTAAGAAACAATTAAGACTAAGCAATGTTGGTATCACCACATCCAAGAGAAAGAGTGAAGAAATTGAATCAAATGCCAAAGGACGCTGCAAGTTTTTCCAGGCTAGAAAACTCGAACAACTCACTCACTTT

Protein sequence

MSSQEPLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYCASSYLEELSSLSFADSKTLKEEKREELFENLKANESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVSKVLGMGVLLTEVYVDTVGDPEKYRIKLSERFPNVKFVVAKKADSLYPVVSGASIVAKVTRDRALREWALEETAENMHRNFGSGYPGDPTTKSWLEDHRHSVFGFPTLVRFSWGTCNPYFKDIVEVSWESDKVDEDGANAGSGKKQLRLSNVGITTSKRKSEEIESNAKGRCKFFQARKLEQLTHF

Homology

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BLAST of MS015520 vs. NCBI nr
Match: XP_022145114.1 (ribonuclease H2 subunit A [Momordica charantia])

HSP 1 Score: 598.6 bits (1542), Expect = 3.0e-167
Identity = 297/297 (100.00%), Postives = 297/297 (100.00%), Query Frame = 0


Query: 1   MSSQEPLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYCASSYLEELSSLSFADSKTLKEE 60
           MSSQEPLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYCASSYLEELSSLSFADSKTLKEE
Sbjct: 1   MSSQEPLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYCASSYLEELSSLSFADSKTLKEE 60

Query: 61  KREELFENLKANESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVSKVLGMGVL 120
           KREELFENLKANESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVSKVLGMGVL
Sbjct: 61  KREELFENLKANESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVSKVLGMGVL 120

Query: 121 LTEVYVDTVGDPEKYRIKLSERFPNVKFVVAKKADSLYPVVSGASIVAKVTRDRALREWA 180
           LTEVYVDTVGDPEKYRIKLSERFPNVKFVVAKKADSLYPVVSGASIVAKVTRDRALREWA
Sbjct: 121 LTEVYVDTVGDPEKYRIKLSERFPNVKFVVAKKADSLYPVVSGASIVAKVTRDRALREWA 180

Query: 181 LEETAENMHRNFGSGYPGDPTTKSWLEDHRHSVFGFPTLVRFSWGTCNPYFKDIVEVSWE 240
           LEETAENMHRNFGSGYPGDPTTKSWLEDHRHSVFGFPTLVRFSWGTCNPYFKDIVEVSWE
Sbjct: 181 LEETAENMHRNFGSGYPGDPTTKSWLEDHRHSVFGFPTLVRFSWGTCNPYFKDIVEVSWE 240

Query: 241 SDKVDEDGANAGSGKKQLRLSNVGITTSKRKSEEIESNAKGRCKFFQARKLEQLTHF 298
           SDKVDEDGANAGSGKKQLRLSNVGITTSKRKSEEIESNAKGRCKFFQARKLEQLTHF
Sbjct: 241 SDKVDEDGANAGSGKKQLRLSNVGITTSKRKSEEIESNAKGRCKFFQARKLEQLTHF 297

[Close]

BLAST of MS015520 vs. NCBI nr
Match: XP_023527419.1 (ribonuclease H2 subunit A [Cucurbita pepo subsp. pepo])

HSP 1 Score: 571.6 bits (1472), Expect = 3.9e-159
Identity = 281/297 (94.61%), Postives = 288/297 (96.97%), Query Frame = 0


Query: 1   MSSQEPLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYCASSYLEELSSLSFADSKTLKEE 60
           MSSQ+PLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYC  SYL+ELSSL+FADSKTLKEE
Sbjct: 1   MSSQDPLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYCTRSYLKELSSLNFADSKTLKEE 60

Query: 61  KREELFENLKANESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVSKVLGMGVL 120
           KREELFE LKANESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLV+KVLGMGVL
Sbjct: 61  KREELFEGLKANESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVTKVLGMGVL 120

Query: 121 LTEVYVDTVGDPEKYRIKLSERFPNVKFVVAKKADSLYPVVSGASIVAKVTRDRALREWA 180
           LTEVYVDTVGDPEKYRIKLSE+FPNVKFVVAKKADSLYPVVSGASIVAKVTRDR LR+WA
Sbjct: 121 LTEVYVDTVGDPEKYRIKLSEKFPNVKFVVAKKADSLYPVVSGASIVAKVTRDRVLRDWA 180

Query: 181 LEETAENMHRNFGSGYPGDPTTKSWLEDHRHSVFGFPTLVRFSWGTCNPYFKDIVEVSWE 240
           LEETAENMHRNFGSGYPGDPTTK WLEDH+HSVFGFPTLVRFSWGTC  YFKDIVEVSWE
Sbjct: 181 LEETAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFPTLVRFSWGTCTSYFKDIVEVSWE 240

Query: 241 SDKVDEDGANAGSGKKQLRLSNVGITTSKRKSEEIESNAKGRCKFFQARKLEQLTHF 298
           SDKVDEDGA  GSGKKQLRLSNVGITTSKRKSEEIESNAKGRCKFFQARKLEQLTHF
Sbjct: 241 SDKVDEDGATGGSGKKQLRLSNVGITTSKRKSEEIESNAKGRCKFFQARKLEQLTHF 297

[Close]

BLAST of MS015520 vs. NCBI nr
Match: XP_038902264.1 (ribonuclease H2 subunit A [Benincasa hispida])

HSP 1 Score: 570.1 bits (1468), Expect = 1.1e-158
Identity = 281/297 (94.61%), Postives = 291/297 (97.98%), Query Frame = 0


Query: 1   MSSQEPLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYCASSYLEELSSLSFADSKTLKEE 60
           MSSQ P+PKWASKPCVMGIDEAGRGPVLGPMVYGCLYCASSYL+ELSSL+FADSKTLKEE
Sbjct: 1   MSSQYPVPKWASKPCVMGIDEAGRGPVLGPMVYGCLYCASSYLKELSSLNFADSKTLKEE 60

Query: 61  KREELFENLKANESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVSKVLGMGVL 120
           KREELFE+LKANESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLV+KVLG+GVL
Sbjct: 61  KREELFEDLKANESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVTKVLGIGVL 120

Query: 121 LTEVYVDTVGDPEKYRIKLSERFPNVKFVVAKKADSLYPVVSGASIVAKVTRDRALREWA 180
           LTEVYVDTVGDPEKYRIKLSE+FP+VKFVVAKKADSLYPVVSGASIVAKVTRDRALREWA
Sbjct: 121 LTEVYVDTVGDPEKYRIKLSEKFPSVKFVVAKKADSLYPVVSGASIVAKVTRDRALREWA 180

Query: 181 LEETAENMHRNFGSGYPGDPTTKSWLEDHRHSVFGFPTLVRFSWGTCNPYFKDIVEVSWE 240
           LEETAENMHRNFGSGYPGDPTTK WLEDH+HSVFGFPTLVRFSWGTC  YFKDIVEVSWE
Sbjct: 181 LEETAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFPTLVRFSWGTCTSYFKDIVEVSWE 240

Query: 241 SDKVDEDGANAGSGKKQLRLSNVGITTSKRKSEEIESNAKGRCKFFQARKLEQLTHF 298
           SDKVDEDGA  GSGKKQLRLS+VGITTSKRKSEEIESNAKGRCKFFQARKLEQLTHF
Sbjct: 241 SDKVDEDGATTGSGKKQLRLSSVGITTSKRKSEEIESNAKGRCKFFQARKLEQLTHF 297

[Close]

BLAST of MS015520 vs. NCBI nr
Match: XP_022979887.1 (ribonuclease H2 subunit A [Cucurbita maxima])

HSP 1 Score: 568.9 bits (1465), Expect = 2.5e-158
Identity = 279/297 (93.94%), Postives = 288/297 (96.97%), Query Frame = 0


Query: 1   MSSQEPLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYCASSYLEELSSLSFADSKTLKEE 60
           MSSQ+PLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYC  SYL+ELSSL+FADSKTLKEE
Sbjct: 1   MSSQDPLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYCTRSYLKELSSLNFADSKTLKEE 60

Query: 61  KREELFENLKANESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVSKVLGMGVL 120
           KREELFE LKANESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLV+KVLGMGVL
Sbjct: 61  KREELFEGLKANESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVTKVLGMGVL 120

Query: 121 LTEVYVDTVGDPEKYRIKLSERFPNVKFVVAKKADSLYPVVSGASIVAKVTRDRALREWA 180
           LTEVYVDTVGDPEKYRIKLSE+FPNVKFVV+KKADSLYPVVSGASIVAKVTRDR LR+WA
Sbjct: 121 LTEVYVDTVGDPEKYRIKLSEKFPNVKFVVSKKADSLYPVVSGASIVAKVTRDRVLRDWA 180

Query: 181 LEETAENMHRNFGSGYPGDPTTKSWLEDHRHSVFGFPTLVRFSWGTCNPYFKDIVEVSWE 240
           LEETAENMHRNFGSGYPGDPTTK WLEDH+HSVFGFP+LVRFSWGTC  YFKDIVEVSWE
Sbjct: 181 LEETAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFPSLVRFSWGTCTSYFKDIVEVSWE 240

Query: 241 SDKVDEDGANAGSGKKQLRLSNVGITTSKRKSEEIESNAKGRCKFFQARKLEQLTHF 298
           SDKVDEDGA  GSGKKQLRLSNVGITTSKRKSEEIESNAKGRCKFFQARKLEQLTHF
Sbjct: 241 SDKVDEDGATGGSGKKQLRLSNVGITTSKRKSEEIESNAKGRCKFFQARKLEQLTHF 297

[Close]

BLAST of MS015520 vs. NCBI nr
Match: XP_022955431.1 (ribonuclease H2 subunit A [Cucurbita moschata])

HSP 1 Score: 568.2 bits (1463), Expect = 4.3e-158
Identity = 279/297 (93.94%), Postives = 286/297 (96.30%), Query Frame = 0


Query: 1   MSSQEPLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYCASSYLEELSSLSFADSKTLKEE 60
           MSSQ+PLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYC  SYL+ELSSL+FADSK LKEE
Sbjct: 1   MSSQDPLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYCTRSYLKELSSLNFADSKALKEE 60

Query: 61  KREELFENLKANESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVSKVLGMGVL 120
           KREELFE LK NESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLV+KVLGMGVL
Sbjct: 61  KREELFEGLKTNESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVTKVLGMGVL 120

Query: 121 LTEVYVDTVGDPEKYRIKLSERFPNVKFVVAKKADSLYPVVSGASIVAKVTRDRALREWA 180
           LTEVYVDTVGDPEKYRIKLSE+FPNVKFVVAKKADSLYPVVSGASIVAKVTRDR LR+WA
Sbjct: 121 LTEVYVDTVGDPEKYRIKLSEKFPNVKFVVAKKADSLYPVVSGASIVAKVTRDRVLRDWA 180

Query: 181 LEETAENMHRNFGSGYPGDPTTKSWLEDHRHSVFGFPTLVRFSWGTCNPYFKDIVEVSWE 240
           LEETAENMHRNFGSGYPGDPTTK WLEDH+HSVFGFPTLVRFSWGTC  YFKDIVEVSWE
Sbjct: 181 LEETAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFPTLVRFSWGTCTSYFKDIVEVSWE 240

Query: 241 SDKVDEDGANAGSGKKQLRLSNVGITTSKRKSEEIESNAKGRCKFFQARKLEQLTHF 298
           SDKVDEDGA  GSGKKQLRLSNVGITTSKRKSEEIESNAKGRCKFFQARKLEQLTHF
Sbjct: 241 SDKVDEDGATGGSGKKQLRLSNVGITTSKRKSEEIESNAKGRCKFFQARKLEQLTHF 297

[Close]

BLAST of MS015520 vs. ExPASy Swiss-Prot
Match: Q9SEZ6 (Ribonuclease H2 subunit A OS=Arabidopsis thaliana OX=3702 GN=At2g25100 PE=2 SV=2)

HSP 1 Score: 443.7 bits (1140), Expect = 1.6e-123
Identity = 212/297 (71.38%), Postives = 256/297 (86.20%), Query Frame = 0


Query: 1   MSSQEPLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYCASSYLEELSSLSFADSKTLKEE 60
           M S+   P+WAS+PC+MGIDEAGRGPVLGPMVYGC+YC  SY   L+SL FADSKTLKEE
Sbjct: 1   MESECLTPEWASQPCLMGIDEAGRGPVLGPMVYGCMYCPISYQSSLASLHFADSKTLKEE 60

Query: 61  KREELFENLKANESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVSKVLGMGVL 120
           KREEL+E+LK ++S+GWAVDVIDPRELS+KML K K NLNEISH+SAMGL+ +VL MGVL
Sbjct: 61  KREELYESLKLDKSLGWAVDVIDPRELSAKMLAKNKTNLNEISHNSAMGLIKRVLDMGVL 120

Query: 121 LTEVYVDTVGDPEKYRIKLSERFPNVKFVVAKKADSLYPVVSGASIVAKVTRDRALREWA 180
           LTE Y+DTVGDP+KYRIKLSERFP++KFVV+KKADSL+P+VSGASIVAKVTRDRAL+EW 
Sbjct: 121 LTEAYLDTVGDPDKYRIKLSERFPSIKFVVSKKADSLFPIVSGASIVAKVTRDRALKEWL 180

Query: 181 LEETAENMHRNFGSGYPGDPTTKSWLEDHRHSVFGFPTLVRFSWGTCNPYFKDIVEVSWE 240
           +EET E+++RNFGSGYPGDP TK+WL  H+HSVFGFP+LVRFSWGTC  + K  VEV+WE
Sbjct: 181 VEETGEDINRNFGSGYPGDPETKAWLVQHKHSVFGFPSLVRFSWGTCTTHLKGEVEVAWE 240

Query: 241 SDKVDEDGANAGSGKKQLRLSNVGITTSKRKSEEIESNAKGRCKFFQARKLEQLTHF 298
           +D+ +E G N  S K+Q +LS+ G  T +++SEEIES+ KGRCKF QARK++QLT F
Sbjct: 241 ADENEESG-NGSSSKRQAKLSSFGFKTCEKRSEEIESSGKGRCKFLQARKIQQLTQF 296

[Close]

BLAST of MS015520 vs. ExPASy Swiss-Prot
Match: Q5U209 (Ribonuclease H2 subunit A OS=Rattus norvegicus OX=10116 GN=Rnaseh2a PE=2 SV=1)

HSP 1 Score: 256.1 bits (653), Expect = 4.7e-67
Identity = 123/250 (49.20%), Postives = 166/250 (66.40%), Query Frame = 0


Query: 6   PLPKWASK-PCVMGIDEAGRGPVLGPMVYGCLYCASSYLEELSSLSFADSKTLKEEKREE 65
           P+P    K PCV+G+DEAGRGPVLGPMVY   YC  S L +L +L  ADSKTL E +RE 
Sbjct: 19  PVPAVCLKEPCVLGVDEAGRGPVLGPMVYAICYCPLSRLADLEALKVADSKTLTENERER 78

Query: 66  LFENLKAN-ESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVSKVLGMGVLLTE 125
           LF  ++ + + +GWA+D++ P  +S+ ML +VK NLN +SHD+A GL+   +   V +T+
Sbjct: 79  LFAKMEEDGDFVGWALDILSPNLISTSMLGRVKYNLNSMSHDTAAGLIQHAMDQNVKVTQ 138

Query: 126 VYVDTVGDPEKYRIKLSERFPNVKFVVAKKADSLYPVVSGASIVAKVTRDRALREWALEE 185
           V+VDTVG PE Y+ +L +RFP ++  V  KADSL+PVVS ASI+AKV RD+A++ W   E
Sbjct: 139 VFVDTVGMPETYQARLQQRFPGIEVTVKAKADSLFPVVSAASIIAKVARDQAVKNWQFVE 198

Query: 186 TAENMHRNFGSGYPGDPTTKSWLEDHRHSVFGFPTLVRFSWGTCNPYF-KDIVEVSWESD 245
           + + +  ++GSGYP DP TK+WL  H   VFGFP  VRFSW T      K+   V+WE  
Sbjct: 199 SLQGLDSDYGSGYPNDPKTKAWLRKHVDPVFGFPQFVRFSWSTAQAILEKEAESVTWEDS 258

Query: 246 KVDEDGANAG 253
             +ED    G
Sbjct: 259 AAEEDPEGPG 268

[Close]

BLAST of MS015520 vs. ExPASy Swiss-Prot
Match: Q9CWY8 (Ribonuclease H2 subunit A OS=Mus musculus OX=10090 GN=Rnaseh2a PE=1 SV=2)

HSP 1 Score: 254.2 bits (648), Expect = 1.8e-66
Identity = 124/250 (49.60%), Postives = 165/250 (66.00%), Query Frame = 0


Query: 6   PLPKWASK-PCVMGIDEAGRGPVLGPMVYGCLYCASSYLEELSSLSFADSKTLKEEKREE 65
           P+P    K PCV+G+DEAGRGPVLGPMVY   YC  S L +L +L  ADSKTL E +RE 
Sbjct: 19  PVPAVCLKEPCVLGVDEAGRGPVLGPMVYAICYCPLSRLADLEALKVADSKTLTENERER 78

Query: 66  LFENLKAN-ESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVSKVLGMGVLLTE 125
           LF  ++ + + +GWA+DV+ P  +S+ ML +VK NLN +SHD+A GL+   L   V +T+
Sbjct: 79  LFAKMEEDGDFVGWALDVLSPNLISTSMLGRVKYNLNSLSHDTAAGLIQYALDQNVNVTQ 138

Query: 126 VYVDTVGDPEKYRIKLSERFPNVKFVVAKKADSLYPVVSGASIVAKVTRDRALREWALEE 185
           V+VDTVG PE Y+ +L + FP ++  V  KADSL+PVVS ASI AKV RD+A++ W   E
Sbjct: 139 VFVDTVGMPETYQARLQQHFPGIEVTVKAKADSLFPVVSAASIFAKVARDKAVKNWQFVE 198

Query: 186 TAENMHRNFGSGYPGDPTTKSWLEDHRHSVFGFPTLVRFSWGTCNPYF-KDIVEVSWESD 245
             +++  ++GSGYP DP TK+WL  H   VFGFP  VRFSW T      K+  +V WE  
Sbjct: 199 NLQDLDSDYGSGYPNDPKTKAWLRKHVDPVFGFPQFVRFSWSTAQAILEKEAEDVIWEDS 258

Query: 246 KVDEDGANAG 253
           + +ED    G
Sbjct: 259 EAEEDPERPG 268

[Close]

BLAST of MS015520 vs. ExPASy Swiss-Prot
Match: O75792 (Ribonuclease H2 subunit A OS=Homo sapiens OX=9606 GN=RNASEH2A PE=1 SV=2)

HSP 1 Score: 252.3 bits (643), Expect = 6.8e-66
Identity = 132/292 (45.21%), Postives = 182/292 (62.33%), Query Frame = 0


Query: 6   PLPKWASK-PCVMGIDEAGRGPVLGPMVYGCLYCASSYLEELSSLSFADSKTLKEEKREE 65
           P+P    K PCV+G+DEAGRGPVLGPMVY   YC    L +L +L  ADSKTL E +RE 
Sbjct: 19  PVPAVCRKEPCVLGVDEAGRGPVLGPMVYAICYCPLPRLADLEALKVADSKTLLESERER 78

Query: 66  LFENLKANESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVSKVLGMGVLLTEV 125
           LF  ++  + +GWA+DV+ P  +S+ ML +VK NLN +SHD+A GL+   L  GV +T+V
Sbjct: 79  LFAKMEDTDFVGWALDVLSPNLISTSMLGRVKYNLNSLSHDTATGLIQYALDQGVNVTQV 138

Query: 126 YVDTVGDPEKYRIKLSERFPNVKFVVAKKADSLYPVVSGASIVAKVTRDRALREWALEET 185
           +VDTVG PE Y+ +L + FP ++  V  KAD+LYPVVS ASI AKV RD+A+++W   E 
Sbjct: 139 FVDTVGMPETYQARLQQSFPGIEVTVKAKADALYPVVSAASICAKVARDQAVKKWQFVEK 198

Query: 186 AENMHRNFGSGYPGDPTTKSWLEDHRHSVFGFPTLVRFSWGTCNPYF-KDIVEVSWESDK 245
            +++  ++GSGYP DP TK+WL++H   VFGFP  VRFSW T      K+  +V WE   
Sbjct: 199 LQDLDTDYGSGYPNDPKTKAWLKEHVEPVFGFPQFVRFSWRTAQTILEKEAEDVIWED-- 258

Query: 246 VDEDGANAGSGKKQLRLSNVGITTSKRKSEEIESNAKGRCKFFQARKLEQLT 296
                 +A   ++ LR       TS   +E  ++  +   ++F  R LE  T
Sbjct: 259 ------SASENQEGLRK-----ITSYFLNEGSQARPRSSHRYFLERGLESAT 297

[Close]

BLAST of MS015520 vs. ExPASy Swiss-Prot
Match: Q2TBT5 (Ribonuclease H2 subunit A OS=Bos taurus OX=9913 GN=RNASEH2A PE=1 SV=1)

HSP 1 Score: 250.0 bits (637), Expect = 3.4e-65
Identity = 137/297 (46.13%), Postives = 181/297 (60.94%), Query Frame = 0


Query: 1   MSSQEPLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYCASSYLEELSSLSFADSKTLKEE 60
           +SS  P P    +PCV+G+DEAGRGPVLGPMVY   YC  S LE+L +L  ADSKTL E 
Sbjct: 16  LSSPVP-PVCLKEPCVLGVDEAGRGPVLGPMVYAICYCPLSRLEDLEALKVADSKTLSES 75

Query: 61  KREELFENLKAN-ESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVSKVLGMGV 120
           +R+ LF  ++ + + +GWA+DV+ P  +S+ ML +VK NLN +SHD+A GLV   L  GV
Sbjct: 76  ERDRLFAKMEEDGDFVGWALDVLSPNLISTSMLGRVKYNLNALSHDTATGLVQFALDQGV 135

Query: 121 LLTEVYVDTVGDPEKYRIKLSERFPNVKFVVAKKADSLYPVVSGASIVAKVTRDRALREW 180
            + +V+VDTVG PE Y+ +L +RFP ++  V  KAD+LYPVVS ASI AKV RD+A++ W
Sbjct: 136 NVAQVFVDTVGLPETYQERLQQRFPGIEVTVKAKADALYPVVSAASICAKVARDQAVKNW 195

Query: 181 ALEETAENMHRNFGSGYPGDPTTKSWLEDHRHSVFGFPTLVRFSWGTCNPYFKDIVE-VS 240
              E  +++  ++GSGYP DP TK+WL  H   VFGFP  VRFSW T     +   E V 
Sbjct: 196 KFVEKLQDLDTDYGSGYPNDPKTKAWLRKHVDPVFGFPQFVRFSWRTAQSILESEAEDVK 255

Query: 241 WESDKVDEDGANAGSGKKQLRLSNVGITTSKRKSEEIESNAKGRCKFFQARKLEQLT 296
           WE     E G   G GK                SE  ++  +   ++FQ R LE  T
Sbjct: 256 WED---SETGDPKGPGK-----------IKSYFSESPQTCLRLPHRYFQERGLESAT 297

[Close]

BLAST of MS015520 vs. ExPASy TrEMBL
Match: A0A6J1CU93 (Ribonuclease OS=Momordica charantia OX=3673 GN=LOC111014631 PE=3 SV=1)

HSP 1 Score: 598.6 bits (1542), Expect = 1.4e-167
Identity = 297/297 (100.00%), Postives = 297/297 (100.00%), Query Frame = 0


Query: 1   MSSQEPLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYCASSYLEELSSLSFADSKTLKEE 60
           MSSQEPLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYCASSYLEELSSLSFADSKTLKEE
Sbjct: 1   MSSQEPLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYCASSYLEELSSLSFADSKTLKEE 60

Query: 61  KREELFENLKANESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVSKVLGMGVL 120
           KREELFENLKANESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVSKVLGMGVL
Sbjct: 61  KREELFENLKANESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVSKVLGMGVL 120

Query: 121 LTEVYVDTVGDPEKYRIKLSERFPNVKFVVAKKADSLYPVVSGASIVAKVTRDRALREWA 180
           LTEVYVDTVGDPEKYRIKLSERFPNVKFVVAKKADSLYPVVSGASIVAKVTRDRALREWA
Sbjct: 121 LTEVYVDTVGDPEKYRIKLSERFPNVKFVVAKKADSLYPVVSGASIVAKVTRDRALREWA 180

Query: 181 LEETAENMHRNFGSGYPGDPTTKSWLEDHRHSVFGFPTLVRFSWGTCNPYFKDIVEVSWE 240
           LEETAENMHRNFGSGYPGDPTTKSWLEDHRHSVFGFPTLVRFSWGTCNPYFKDIVEVSWE
Sbjct: 181 LEETAENMHRNFGSGYPGDPTTKSWLEDHRHSVFGFPTLVRFSWGTCNPYFKDIVEVSWE 240

Query: 241 SDKVDEDGANAGSGKKQLRLSNVGITTSKRKSEEIESNAKGRCKFFQARKLEQLTHF 298
           SDKVDEDGANAGSGKKQLRLSNVGITTSKRKSEEIESNAKGRCKFFQARKLEQLTHF
Sbjct: 241 SDKVDEDGANAGSGKKQLRLSNVGITTSKRKSEEIESNAKGRCKFFQARKLEQLTHF 297

[Close]

BLAST of MS015520 vs. ExPASy TrEMBL
Match: A0A6J1IPY0 (Ribonuclease OS=Cucurbita maxima OX=3661 GN=LOC111479443 PE=3 SV=1)

HSP 1 Score: 568.9 bits (1465), Expect = 1.2e-158
Identity = 279/297 (93.94%), Postives = 288/297 (96.97%), Query Frame = 0


Query: 1   MSSQEPLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYCASSYLEELSSLSFADSKTLKEE 60
           MSSQ+PLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYC  SYL+ELSSL+FADSKTLKEE
Sbjct: 1   MSSQDPLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYCTRSYLKELSSLNFADSKTLKEE 60

Query: 61  KREELFENLKANESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVSKVLGMGVL 120
           KREELFE LKANESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLV+KVLGMGVL
Sbjct: 61  KREELFEGLKANESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVTKVLGMGVL 120

Query: 121 LTEVYVDTVGDPEKYRIKLSERFPNVKFVVAKKADSLYPVVSGASIVAKVTRDRALREWA 180
           LTEVYVDTVGDPEKYRIKLSE+FPNVKFVV+KKADSLYPVVSGASIVAKVTRDR LR+WA
Sbjct: 121 LTEVYVDTVGDPEKYRIKLSEKFPNVKFVVSKKADSLYPVVSGASIVAKVTRDRVLRDWA 180

Query: 181 LEETAENMHRNFGSGYPGDPTTKSWLEDHRHSVFGFPTLVRFSWGTCNPYFKDIVEVSWE 240
           LEETAENMHRNFGSGYPGDPTTK WLEDH+HSVFGFP+LVRFSWGTC  YFKDIVEVSWE
Sbjct: 181 LEETAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFPSLVRFSWGTCTSYFKDIVEVSWE 240

Query: 241 SDKVDEDGANAGSGKKQLRLSNVGITTSKRKSEEIESNAKGRCKFFQARKLEQLTHF 298
           SDKVDEDGA  GSGKKQLRLSNVGITTSKRKSEEIESNAKGRCKFFQARKLEQLTHF
Sbjct: 241 SDKVDEDGATGGSGKKQLRLSNVGITTSKRKSEEIESNAKGRCKFFQARKLEQLTHF 297

[Close]

BLAST of MS015520 vs. ExPASy TrEMBL
Match: A0A6J1GTR5 (Ribonuclease OS=Cucurbita moschata OX=3662 GN=LOC111457459 PE=3 SV=1)

HSP 1 Score: 568.2 bits (1463), Expect = 2.1e-158
Identity = 279/297 (93.94%), Postives = 286/297 (96.30%), Query Frame = 0


Query: 1   MSSQEPLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYCASSYLEELSSLSFADSKTLKEE 60
           MSSQ+PLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYC  SYL+ELSSL+FADSK LKEE
Sbjct: 1   MSSQDPLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYCTRSYLKELSSLNFADSKALKEE 60

Query: 61  KREELFENLKANESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVSKVLGMGVL 120
           KREELFE LK NESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLV+KVLGMGVL
Sbjct: 61  KREELFEGLKTNESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVTKVLGMGVL 120

Query: 121 LTEVYVDTVGDPEKYRIKLSERFPNVKFVVAKKADSLYPVVSGASIVAKVTRDRALREWA 180
           LTEVYVDTVGDPEKYRIKLSE+FPNVKFVVAKKADSLYPVVSGASIVAKVTRDR LR+WA
Sbjct: 121 LTEVYVDTVGDPEKYRIKLSEKFPNVKFVVAKKADSLYPVVSGASIVAKVTRDRVLRDWA 180

Query: 181 LEETAENMHRNFGSGYPGDPTTKSWLEDHRHSVFGFPTLVRFSWGTCNPYFKDIVEVSWE 240
           LEETAENMHRNFGSGYPGDPTTK WLEDH+HSVFGFPTLVRFSWGTC  YFKDIVEVSWE
Sbjct: 181 LEETAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFPTLVRFSWGTCTSYFKDIVEVSWE 240

Query: 241 SDKVDEDGANAGSGKKQLRLSNVGITTSKRKSEEIESNAKGRCKFFQARKLEQLTHF 298
           SDKVDEDGA  GSGKKQLRLSNVGITTSKRKSEEIESNAKGRCKFFQARKLEQLTHF
Sbjct: 241 SDKVDEDGATGGSGKKQLRLSNVGITTSKRKSEEIESNAKGRCKFFQARKLEQLTHF 297

[Close]

BLAST of MS015520 vs. ExPASy TrEMBL
Match: A0A5A7TT92 (Ribonuclease OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold130G001310 PE=3 SV=1)

HSP 1 Score: 562.4 bits (1448), Expect = 1.1e-156
Identity = 277/297 (93.27%), Postives = 287/297 (96.63%), Query Frame = 0


Query: 1   MSSQEPLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYCASSYLEELSSLSFADSKTLKEE 60
           MS Q+ LPKWASKPCVMGIDEAGRGPVLGPMVYGCLYCASSYL+ELSSL+FADSKTLKEE
Sbjct: 1   MSLQDSLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYCASSYLKELSSLNFADSKTLKEE 60

Query: 61  KREELFENLKANESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVSKVLGMGVL 120
           KREELFE+LKANESIGWAVDVIDPRELSSKMLNKVKINLNEISH SAMGLV+KVL MGVL
Sbjct: 61  KREELFEDLKANESIGWAVDVIDPRELSSKMLNKVKINLNEISHGSAMGLVTKVLSMGVL 120

Query: 121 LTEVYVDTVGDPEKYRIKLSERFPNVKFVVAKKADSLYPVVSGASIVAKVTRDRALREWA 180
           LTEVYVDTVGDPEKYRIKLSE+FPN+KFVVAKKADSLYPVVSGASIVAKVTRDRALREWA
Sbjct: 121 LTEVYVDTVGDPEKYRIKLSEKFPNIKFVVAKKADSLYPVVSGASIVAKVTRDRALREWA 180

Query: 181 LEETAENMHRNFGSGYPGDPTTKSWLEDHRHSVFGFPTLVRFSWGTCNPYFKDIVEVSWE 240
           LEETAENMHRNFGSGYPGDPTTK WLEDH+HSVFGFPTLVRFSWGTC  YFKD+VEVSWE
Sbjct: 181 LEETAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFPTLVRFSWGTCTSYFKDVVEVSWE 240

Query: 241 SDKVDEDGANAGSGKKQLRLSNVGITTSKRKSEEIESNAKGRCKFFQARKLEQLTHF 298
           SDKVDEDGA  GSGKKQLRLSNVGITTSKRKSEEIESNAKGRCKFFQARKLEQL+ F
Sbjct: 241 SDKVDEDGATGGSGKKQLRLSNVGITTSKRKSEEIESNAKGRCKFFQARKLEQLSRF 297

[Close]

BLAST of MS015520 vs. ExPASy TrEMBL
Match: A0A1S3BXP1 (Ribonuclease OS=Cucumis melo OX=3656 GN=LOC103494542 PE=3 SV=1)

HSP 1 Score: 562.4 bits (1448), Expect = 1.1e-156
Identity = 277/297 (93.27%), Postives = 287/297 (96.63%), Query Frame = 0


Query: 1   MSSQEPLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYCASSYLEELSSLSFADSKTLKEE 60
           MS Q+ LPKWASKPCVMGIDEAGRGPVLGPMVYGCLYCASSYL+ELSSL+FADSKTLKEE
Sbjct: 1   MSLQDSLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYCASSYLKELSSLNFADSKTLKEE 60

Query: 61  KREELFENLKANESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVSKVLGMGVL 120
           KREELFE+LKANESIGWAVDVIDPRELSSKMLNKVKINLNEISH SAMGLV+KVL MGVL
Sbjct: 61  KREELFEDLKANESIGWAVDVIDPRELSSKMLNKVKINLNEISHGSAMGLVTKVLSMGVL 120

Query: 121 LTEVYVDTVGDPEKYRIKLSERFPNVKFVVAKKADSLYPVVSGASIVAKVTRDRALREWA 180
           LTEVYVDTVGDPEKYRIKLSE+FPN+KFVVAKKADSLYPVVSGASIVAKVTRDRALREWA
Sbjct: 121 LTEVYVDTVGDPEKYRIKLSEKFPNIKFVVAKKADSLYPVVSGASIVAKVTRDRALREWA 180

Query: 181 LEETAENMHRNFGSGYPGDPTTKSWLEDHRHSVFGFPTLVRFSWGTCNPYFKDIVEVSWE 240
           LEETAENMHRNFGSGYPGDPTTK WLEDH+HSVFGFPTLVRFSWGTC  YFKD+VEVSWE
Sbjct: 181 LEETAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFPTLVRFSWGTCTSYFKDVVEVSWE 240

Query: 241 SDKVDEDGANAGSGKKQLRLSNVGITTSKRKSEEIESNAKGRCKFFQARKLEQLTHF 298
           SDKVDEDGA  GSGKKQLRLSNVGITTSKRKSEEIESNAKGRCKFFQARKLEQL+ F
Sbjct: 241 SDKVDEDGATGGSGKKQLRLSNVGITTSKRKSEEIESNAKGRCKFFQARKLEQLSRF 297

[Close]

BLAST of MS015520 vs. TAIR 10
Match: AT2G25100.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 443.7 bits (1140), Expect = 1.1e-124
Identity = 212/297 (71.38%), Postives = 256/297 (86.20%), Query Frame = 0


Query: 1   MSSQEPLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYCASSYLEELSSLSFADSKTLKEE 60
           M S+   P+WAS+PC+MGIDEAGRGPVLGPMVYGC+YC  SY   L+SL FADSKTLKEE
Sbjct: 1   MESECLTPEWASQPCLMGIDEAGRGPVLGPMVYGCMYCPISYQSSLASLHFADSKTLKEE 60

Query: 61  KREELFENLKANESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVSKVLGMGVL 120
           KREEL+E+LK ++S+GWAVDVIDPRELS+KML K K NLNEISH+SAMGL+ +VL MGVL
Sbjct: 61  KREELYESLKLDKSLGWAVDVIDPRELSAKMLAKNKTNLNEISHNSAMGLIKRVLDMGVL 120

Query: 121 LTEVYVDTVGDPEKYRIKLSERFPNVKFVVAKKADSLYPVVSGASIVAKVTRDRALREWA 180
           LTE Y+DTVGDP+KYRIKLSERFP++KFVV+KKADSL+P+VSGASIVAKVTRDRAL+EW 
Sbjct: 121 LTEAYLDTVGDPDKYRIKLSERFPSIKFVVSKKADSLFPIVSGASIVAKVTRDRALKEWL 180

Query: 181 LEETAENMHRNFGSGYPGDPTTKSWLEDHRHSVFGFPTLVRFSWGTCNPYFKDIVEVSWE 240
           +EET E+++RNFGSGYPGDP TK+WL  H+HSVFGFP+LVRFSWGTC  + K  VEV+WE
Sbjct: 181 VEETGEDINRNFGSGYPGDPETKAWLVQHKHSVFGFPSLVRFSWGTCTTHLKGEVEVAWE 240

Query: 241 SDKVDEDGANAGSGKKQLRLSNVGITTSKRKSEEIESNAKGRCKFFQARKLEQLTHF 298
           +D+ +E G N  S K+Q +LS+ G  T +++SEEIES+ KGRCKF QARK++QLT F
Sbjct: 241 ADENEESG-NGSSSKRQAKLSSFGFKTCEKRSEEIESSGKGRCKFLQARKIQQLTQF 296

[Close]

BLAST of MS015520 vs. TAIR 10
Match: AT2G25100.2 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 407.9 bits (1047), Expect = 6.9e-114
Identity = 199/297 (67.00%), Postives = 241/297 (81.14%), Query Frame = 0


Query: 1   MSSQEPLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYCASSYLEELSSLSFADSKTLKEE 60
           M S+   P+WAS+PC+MGIDEAGRGPVLGPMVYGC+YC  SY   L+SL FADSKTLKEE
Sbjct: 1   MESECLTPEWASQPCLMGIDEAGRGPVLGPMVYGCMYCPISYQSSLASLHFADSKTLKEE 60

Query: 61  KREELFENLKANESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVSKVLGMGVL 120
           KREEL+E+LK ++S+GWAVDVIDPRELS+KML                   +KVL MGVL
Sbjct: 61  KREELYESLKLDKSLGWAVDVIDPRELSAKML-------------------AKVLDMGVL 120

Query: 121 LTEVYVDTVGDPEKYRIKLSERFPNVKFVVAKKADSLYPVVSGASIVAKVTRDRALREWA 180
           LTE Y+DTVGDP+KYRIKLSERFP++KFVV+KKADSL+P+VSGASIVAKVTRDRAL+EW 
Sbjct: 121 LTEAYLDTVGDPDKYRIKLSERFPSIKFVVSKKADSLFPIVSGASIVAKVTRDRALKEWL 180

Query: 181 LEETAENMHRNFGSGYPGDPTTKSWLEDHRHSVFGFPTLVRFSWGTCNPYFKDIVEVSWE 240
           +EET E+++RNFGSGYPGDP TK+WL  H+HSVFGFP+LVRFSWGTC  + K  VEV+WE
Sbjct: 181 VEETGEDINRNFGSGYPGDPETKAWLVQHKHSVFGFPSLVRFSWGTCTTHLKGEVEVAWE 240

Query: 241 SDKVDEDGANAGSGKKQLRLSNVGITTSKRKSEEIESNAKGRCKFFQARKLEQLTHF 298
           +D+ +E G N  S K+Q +LS+ G  T +++SEEIES+ KGRCKF QARK++QLT F
Sbjct: 241 ADENEESG-NGSSSKRQAKLSSFGFKTCEKRSEEIESSGKGRCKFLQARKIQQLTQF 277

The following BLAST results are available for this feature:

BLAST of MS015520 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022145114.1	3.0e-167	100.00	ribonuclease H2 subunit A [Momordica charantia]	[more]
XP_023527419.1	3.9e-159	94.61	ribonuclease H2 subunit A [Cucurbita pepo subsp. pepo]	[more]
XP_038902264.1	1.1e-158	94.61	ribonuclease H2 subunit A [Benincasa hispida]	[more]
XP_022979887.1	2.5e-158	93.94	ribonuclease H2 subunit A [Cucurbita maxima]	[more]
XP_022955431.1	4.3e-158	93.94	ribonuclease H2 subunit A [Cucurbita moschata]	[more]

BLAST of MS015520 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SEZ6	1.6e-123	71.38	Ribonuclease H2 subunit A OS=Arabidopsis thaliana OX=3702 GN=At2g25100 PE=2 SV=2	[more]
Q5U209	4.7e-67	49.20	Ribonuclease H2 subunit A OS=Rattus norvegicus OX=10116 GN=Rnaseh2a PE=2 SV=1	[more]
Q9CWY8	1.8e-66	49.60	Ribonuclease H2 subunit A OS=Mus musculus OX=10090 GN=Rnaseh2a PE=1 SV=2	[more]
O75792	6.8e-66	45.21	Ribonuclease H2 subunit A OS=Homo sapiens OX=9606 GN=RNASEH2A PE=1 SV=2	[more]
Q2TBT5	3.4e-65	46.13	Ribonuclease H2 subunit A OS=Bos taurus OX=9913 GN=RNASEH2A PE=1 SV=1	[more]

BLAST of MS015520 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CU93	1.4e-167	100.00	Ribonuclease OS=Momordica charantia OX=3673 GN=LOC111014631 PE=3 SV=1	[more]
A0A6J1IPY0	1.2e-158	93.94	Ribonuclease OS=Cucurbita maxima OX=3661 GN=LOC111479443 PE=3 SV=1	[more]
A0A6J1GTR5	2.1e-158	93.94	Ribonuclease OS=Cucurbita moschata OX=3662 GN=LOC111457459 PE=3 SV=1	[more]
A0A5A7TT92	1.1e-156	93.27	Ribonuclease OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold130G001310 ...	[more]
A0A1S3BXP1	1.1e-156	93.27	Ribonuclease OS=Cucumis melo OX=3656 GN=LOC103494542 PE=3 SV=1	[more]

BLAST of MS015520 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G25100.1	1.1e-124	71.38	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT2G25100.2	6.9e-114	67.00	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR024567	Ribonuclease HII/HIII domain	PFAM	PF01351	RNase_HII	coord: 17..227 e-value: 5.4E-53 score: 179.8
IPR023160	Ribonuclease HII, helix-loop-helix cap domain superfamily	GENE3D	1.10.10.460	Ribonuclease hii. Domain 2	coord: 187..240 e-value: 5.5E-20 score: 72.7
IPR004649	Ribonuclease H2, subunit A	TIGRFAM	TIGR00729	TIGR00729	coord: 16..228 e-value: 1.6E-55 score: 186.3
IPR036397	Ribonuclease H superfamily	GENE3D	3.30.420.10		coord: 1..186 e-value: 1.8E-67 score: 228.8
IPR001352	Ribonuclease HII/HIII	PANTHER	PTHR10954	RIBONUCLEASE H2 SUBUNIT A	coord: 7..296
None	No IPR available	PANTHER	PTHR10954:SF19	RIBONUCLEASE	coord: 7..296
None	No IPR available	CDD	cd07181	RNase_HII_eukaryota_like	coord: 17..236 e-value: 3.39708E-142 score: 397.66
IPR012337	Ribonuclease H-like superfamily	SUPERFAMILY	53098	Ribonuclease H-like	coord: 15..232

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS015520.1	MS015520.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016070	RNA metabolic process
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0003723	RNA binding
molecular_function	GO:0004523	RNA-DNA hybrid ribonuclease activity