MS015493 (gene) Bitter gourd (TR) v1

Overview


Name	MS015493
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	RNase H domain-containing protein
Location	scaffold468: 186214 .. 186546 (-)
RNA-Seq Expression	MS015493
Synteny	MS015493

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

AAAAAAGTTGATGAGGTACATGTTTCGGTTTGTTGGTCAATGCCTCCAAAAGGGGTCTTTAAATGTAACACTGATGCCGCGTGTGACTCTAGAAGAATGAAATCAGGGATCGGGGTTGTCTTTCGAGATGATCAAAATTTCCTTCAGGCGGCGATGCTAGCAACTTTCCCCGAAATATCCCCTCTCTCGGCTAAAGTGCTCGCGATTCGGGAGGCAGTTAGGTTGGCAGCAAGGTTGGGGTGCCCCCGGGTGTTGTTTGAATCAGATTCTTTGATGGTAATACAATTGATTCGAGGCGATTTAGAGTGGCAAGGAGAAATTGCAACTTTGGTT

mRNA sequence

AAAAAAGTTGATGAGGTACATGTTTCGGTTTGTTGGTCAATGCCTCCAAAAGGGGTCTTTAAATGTAACACTGATGCCGCGTGTGACTCTAGAAGAATGAAATCAGGGATCGGGGTTGTCTTTCGAGATGATCAAAATTTCCTTCAGGCGGCGATGCTAGCAACTTTCCCCGAAATATCCCCTCTCTCGGCTAAAGTGCTCGCGATTCGGGAGGCAGTTAGGTTGGCAGCAAGGTTGGGGTGCCCCCGGGTGTTGTTTGAATCAGATTCTTTGATGGTAATACAATTGATTCGAGGCGATTTAGAGTGGCAAGGAGAAATTGCAACTTTGGTT

Coding sequence (CDS)

AAAAAAGTTGATGAGGTACATGTTTCGGTTTGTTGGTCAATGCCTCCAAAAGGGGTCTTTAAATGTAACACTGATGCCGCGTGTGACTCTAGAAGAATGAAATCAGGGATCGGGGTTGTCTTTCGAGATGATCAAAATTTCCTTCAGGCGGCGATGCTAGCAACTTTCCCCGAAATATCCCCTCTCTCGGCTAAAGTGCTCGCGATTCGGGAGGCAGTTAGGTTGGCAGCAAGGTTGGGGTGCCCCCGGGTGTTGTTTGAATCAGATTCTTTGATGGTAATACAATTGATTCGAGGCGATTTAGAGTGGCAAGGAGAAATTGCAACTTTGGTT

Protein sequence

KKVDEVHVSVCWSMPPKGVFKCNTDAACDSRRMKSGIGVVFRDDQNFLQAAMLATFPEISPLSAKVLAIREAVRLAARLGCPRVLFESDSLMVIQLIRGDLEWQGEIATLV

Homology

[Close]

BLAST of MS015493 vs. NCBI nr
Match: XP_022158489.1 (uncharacterized protein LOC111024968 [Momordica charantia])

HSP 1 Score: 71.6 bits (174), Expect = 4.7e-09
Identity = 43/100 (43.00%), Postives = 56/100 (56.00%), Query Frame = 0


Query: 12  WSMPPKGVFKCNTDAACDSRRMKSGIGVVFRDDQNFLQAAMLATFPEISPLSAKVLAIRE 71
           W  P  GV K NTDAA       SG+GV+ R+    +  AM+      +PL AK+LAIRE
Sbjct: 67  WRPPLGGVVKMNTDAAVSEE--GSGVGVLLREGNAEIVGAMVDFHMVKTPLLAKILAIRE 126

Query: 72  AVRLAARLGCPRVLFESDSLMVIQLIRGDLEWQGEIATLV 112
            + LA RLG  RV+ E+DSL  + LI     W+GE  + V
Sbjct: 127 GLSLATRLGVHRVVVETDSLEALNLIGDKSPWKGEAVSWV 164

[Close]

BLAST of MS015493 vs. NCBI nr
Match: GAU48087.1 (hypothetical protein TSUD_238260 [Trifolium subterraneum])

HSP 1 Score: 70.5 bits (171), Expect = 1.0e-08
Identity = 38/101 (37.62%), Postives = 51/101 (50.50%), Query Frame = 0


Query: 12  WSMPPKGVFKCNTDAACDSRRMKSGIGVVFRDDQNFLQAAMLATFP-EISPLSAKVLAIR 71
           W  PP    KCN D A      K GIG+ FRD+      A   TFP E++    +  A++
Sbjct: 198 WEKPPANWLKCNVDGAIFMTERKFGIGICFRDNSGSFVQAHTMTFPFEVTAAECEATAMK 257

Query: 72  EAVRLAARLGCPRVLFESDSLMVIQLIRGDLEWQGEIATLV 112
            A+ LA   G  RVLFESD   V+  +R D  +  E+ TL+
Sbjct: 258 HALALALSNGFERVLFESDCQQVVNALRNDYLYANELGTLL 298

[Close]

BLAST of MS015493 vs. NCBI nr
Match: GAU36089.1 (hypothetical protein TSUD_277000 [Trifolium subterraneum])

HSP 1 Score: 70.1 bits (170), Expect = 1.4e-08
Identity = 38/101 (37.62%), Postives = 51/101 (50.50%), Query Frame = 0


Query: 12  WSMPPKGVFKCNTDAACDSRRMKSGIGVVFRDDQNFLQAAMLATFP-EISPLSAKVLAIR 71
           W  PP    KCN D A      K GIG+ FRD       A   TFP E++ +  +  A++
Sbjct: 799 WEKPPVNWLKCNVDGAIFMTEGKFGIGICFRDSSGSFVQARTMTFPFEVTDVECEATAMK 858

Query: 72  EAVRLAARLGCPRVLFESDSLMVIQLIRGDLEWQGEIATLV 112
            A+ LA   G  RVLFESD   V+  +R D  +  E+ TL+
Sbjct: 859 HALALALSNGFERVLFESDCQQVVNALRNDYLYANELGTLL 899

[Close]

BLAST of MS015493 vs. NCBI nr
Match: XP_022141766.1 (uncharacterized protein LOC111012047 [Momordica charantia])

HSP 1 Score: 69.3 bits (168), Expect = 2.3e-08
Identity = 34/67 (50.75%), Postives = 45/67 (67.16%), Query Frame = 0


Query: 12  WSMPPKGVFKCNTDAACDSRRMKSGIGVVFRDDQNFLQAAMLATFPEISPLSAKVLAIRE 71
           W  P KG  K N DAA D R  + G+GV+ RDD+N + AA++++    SPL A++ AIRE
Sbjct: 88  WKPPSKGTVKLNVDAAFDVRNQRGGVGVLVRDDRNSILAALISSHNVNSPLLAEICAIRE 147

Query: 72  AVRLAAR 79
           AVRLA R
Sbjct: 148 AVRLAER 154

[Close]

BLAST of MS015493 vs. NCBI nr
Match: XP_024195872.1 (uncharacterized protein LOC112199038 [Rosa chinensis])

HSP 1 Score: 68.6 bits (166), Expect = 4.0e-08
Identity = 40/100 (40.00%), Postives = 58/100 (58.00%), Query Frame = 0


Query: 12  WSMPPKGVFKCNTDAACDSRRMKSGIGVVFRDDQ-NFLQAAMLATFPEISPLSAKVLAIR 71
           WS P  GV KCN D A  +   ++  G+VFR++  NFL AA        SP   +V+A+ 
Sbjct: 251 WSAPVSGVLKCNADGAFVAHSRRAAYGLVFRNETGNFLAAAAAPLRQMSSPFHTEVMAML 310

Query: 72  EAVRLAARLGCPRVLFESDSLMVIQLIRGDLEWQGEIATL 111
           E++RLA  L    V+FESD L++IQ +  D   + +I+TL
Sbjct: 311 ESMRLAETLHYKNVIFESDCLLLIQALAND---EPDISTL 347

[Close]

BLAST of MS015493 vs. ExPASy TrEMBL
Match: A0A6J1DZK3 (uncharacterized protein LOC111024968 OS=Momordica charantia OX=3673 GN=LOC111024968 PE=4 SV=1)

HSP 1 Score: 71.6 bits (174), Expect = 2.3e-09
Identity = 43/100 (43.00%), Postives = 56/100 (56.00%), Query Frame = 0


Query: 12  WSMPPKGVFKCNTDAACDSRRMKSGIGVVFRDDQNFLQAAMLATFPEISPLSAKVLAIRE 71
           W  P  GV K NTDAA       SG+GV+ R+    +  AM+      +PL AK+LAIRE
Sbjct: 67  WRPPLGGVVKMNTDAAVSEE--GSGVGVLLREGNAEIVGAMVDFHMVKTPLLAKILAIRE 126

Query: 72  AVRLAARLGCPRVLFESDSLMVIQLIRGDLEWQGEIATLV 112
            + LA RLG  RV+ E+DSL  + LI     W+GE  + V
Sbjct: 127 GLSLATRLGVHRVVVETDSLEALNLIGDKSPWKGEAVSWV 164

[Close]

BLAST of MS015493 vs. ExPASy TrEMBL
Match: A0A2Z6PPU2 (Reverse transcriptase domain-containing protein OS=Trifolium subterraneum OX=3900 GN=TSUD_238260 PE=4 SV=1)

HSP 1 Score: 70.5 bits (171), Expect = 5.1e-09
Identity = 38/101 (37.62%), Postives = 51/101 (50.50%), Query Frame = 0


Query: 12  WSMPPKGVFKCNTDAACDSRRMKSGIGVVFRDDQNFLQAAMLATFP-EISPLSAKVLAIR 71
           W  PP    KCN D A      K GIG+ FRD+      A   TFP E++    +  A++
Sbjct: 198 WEKPPANWLKCNVDGAIFMTERKFGIGICFRDNSGSFVQAHTMTFPFEVTAAECEATAMK 257

Query: 72  EAVRLAARLGCPRVLFESDSLMVIQLIRGDLEWQGEIATLV 112
            A+ LA   G  RVLFESD   V+  +R D  +  E+ TL+
Sbjct: 258 HALALALSNGFERVLFESDCQQVVNALRNDYLYANELGTLL 298

[Close]

BLAST of MS015493 vs. ExPASy TrEMBL
Match: A0A2Z6NX36 (Reverse transcriptase domain-containing protein OS=Trifolium subterraneum OX=3900 GN=TSUD_277000 PE=4 SV=1)

HSP 1 Score: 70.1 bits (170), Expect = 6.6e-09
Identity = 38/101 (37.62%), Postives = 51/101 (50.50%), Query Frame = 0


Query: 12  WSMPPKGVFKCNTDAACDSRRMKSGIGVVFRDDQNFLQAAMLATFP-EISPLSAKVLAIR 71
           W  PP    KCN D A      K GIG+ FRD       A   TFP E++ +  +  A++
Sbjct: 799 WEKPPVNWLKCNVDGAIFMTEGKFGIGICFRDSSGSFVQARTMTFPFEVTDVECEATAMK 858

Query: 72  EAVRLAARLGCPRVLFESDSLMVIQLIRGDLEWQGEIATLV 112
            A+ LA   G  RVLFESD   V+  +R D  +  E+ TL+
Sbjct: 859 HALALALSNGFERVLFESDCQQVVNALRNDYLYANELGTLL 899

[Close]

BLAST of MS015493 vs. ExPASy TrEMBL
Match: A0A6J1CK80 (uncharacterized protein LOC111012047 OS=Momordica charantia OX=3673 GN=LOC111012047 PE=4 SV=1)

HSP 1 Score: 69.3 bits (168), Expect = 1.1e-08
Identity = 34/67 (50.75%), Postives = 45/67 (67.16%), Query Frame = 0


Query: 12  WSMPPKGVFKCNTDAACDSRRMKSGIGVVFRDDQNFLQAAMLATFPEISPLSAKVLAIRE 71
           W  P KG  K N DAA D R  + G+GV+ RDD+N + AA++++    SPL A++ AIRE
Sbjct: 88  WKPPSKGTVKLNVDAAFDVRNQRGGVGVLVRDDRNSILAALISSHNVNSPLLAEICAIRE 147

Query: 72  AVRLAAR 79
           AVRLA R
Sbjct: 148 AVRLAER 154

[Close]

BLAST of MS015493 vs. ExPASy TrEMBL
Match: A0A2Z6MC97 (RNase H domain-containing protein OS=Trifolium subterraneum OX=3900 GN=TSUD_381600 PE=4 SV=1)

HSP 1 Score: 68.6 bits (166), Expect = 1.9e-08
Identity = 38/101 (37.62%), Postives = 51/101 (50.50%), Query Frame = 0


Query: 12  WSMPPKGVFKCNTDAACDSRRMKSGIGVVFRDDQNFLQAAMLATFP-EISPLSAKVLAIR 71
           W  PP    KCN D A      K GIG+ FRD       A   TFP E++ +  +  A++
Sbjct: 414 WEKPPVNWLKCNVDGAIFMTEGKFGIGICFRDSSGSFVQAHTMTFPFEVTAVECEATAMK 473

Query: 72  EAVRLAARLGCPRVLFESDSLMVIQLIRGDLEWQGEIATLV 112
            A+ LA   G  RVLFESD   V+  +R D  +  E+ TL+
Sbjct: 474 LALALALSNGFERVLFESDCQQVVNALRNDYLYANELGTLL 514

[Close]

BLAST of MS015493 vs. TAIR 10
Match: AT2G34320.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 55.1 bits (131), Expect = 4.3e-08
Identity = 32/100 (32.00%), Postives = 50/100 (50.00%), Query Frame = 0


Query: 5   EVHVSVCWSMPPKGVFKCNTDAACDSRRMKSGIGVVFRDDQNFLQAAMLATFPEI-SPLS 64
           E ++SV W  PP    KCNTDA       + GIG + R++   +        P   + L 
Sbjct: 130 ERNLSVQWKAPPYQWVKCNTDATWQLENPRCGIGWILRNESGGVLWMGARALPRTKNVLE 189

Query: 65  AKVLAIREAVRLAARLGCPRVLFESDSLMVIQLIRGDLEW 104
           A++ A+R AV   +R    R++FESD+  ++ L+  D  W
Sbjct: 190 AELEALRWAVLTMSRFNYKRIIFESDAQALVNLLNSDDFW 229

[Close]

BLAST of MS015493 vs. TAIR 10
Match: AT4G29090.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 55.1 bits (131), Expect = 4.3e-08
Identity = 35/102 (34.31%), Postives = 56/102 (54.90%), Query Frame = 0


Query: 5   EVHVSVC--WSMPPKGVFKCNTDAACDSRRMKSGIGVVFRDDQNFLQAAMLATFPEI-SP 64
           +V+ S C  W  PP    KCNTDA  +    + GIG V R+++  ++       P++ S 
Sbjct: 411 QVNRSSCGRWRPPPHQWVKCNTDATWNRDNERCGIGWVLRNEKGEVKWMGARALPKLKSV 470

Query: 65  LSAKVLAIREAVRLAARLGCPRVLFESDSLMVIQLIRGDLEW 104
           L A++ A+R AV   +R     V+FESDS ++I+++  D  W
Sbjct: 471 LEAELEAMRWAVLSLSRFQYNYVIFESDSQVLIEILNNDEIW 512

The following BLAST results are available for this feature:

BLAST of MS015493 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022158489.1	4.7e-09	43.00	uncharacterized protein LOC111024968 [Momordica charantia]	[more]
GAU48087.1	1.0e-08	37.62	hypothetical protein TSUD_238260 [Trifolium subterraneum]	[more]
GAU36089.1	1.4e-08	37.62	hypothetical protein TSUD_277000 [Trifolium subterraneum]	[more]
XP_022141766.1	2.3e-08	50.75	uncharacterized protein LOC111012047 [Momordica charantia]	[more]
XP_024195872.1	4.0e-08	40.00	uncharacterized protein LOC112199038 [Rosa chinensis]	[more]

BLAST of MS015493 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of MS015493 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DZK3	2.3e-09	43.00	uncharacterized protein LOC111024968 OS=Momordica charantia OX=3673 GN=LOC111024...	[more]
A0A2Z6PPU2	5.1e-09	37.62	Reverse transcriptase domain-containing protein OS=Trifolium subterraneum OX=390...	[more]
A0A2Z6NX36	6.6e-09	37.62	Reverse transcriptase domain-containing protein OS=Trifolium subterraneum OX=390...	[more]
A0A6J1CK80	1.1e-08	50.75	uncharacterized protein LOC111012047 OS=Momordica charantia OX=3673 GN=LOC111012...	[more]
A0A2Z6MC97	1.9e-08	37.62	RNase H domain-containing protein OS=Trifolium subterraneum OX=3900 GN=TSUD_3816...	[more]

BLAST of MS015493 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G34320.1	4.3e-08	32.00	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT4G29090.1	4.3e-08	34.31	Ribonuclease H-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002156	Ribonuclease H domain	PFAM	PF13456	RVT_3	coord: 23..102 e-value: 3.0E-13 score: 49.7
IPR036397	Ribonuclease H superfamily	GENE3D	3.30.420.10		coord: 18..110 e-value: 4.7E-11 score: 44.8
None	No IPR available	PANTHER	PTHR47074	BNAC02G40300D PROTEIN	coord: 8..106
IPR044730	Ribonuclease H-like domain, plant type	CDD	cd06222	RNase_H_like	coord: 22..111 e-value: 7.91718E-14 score: 60.7908
IPR012337	Ribonuclease H-like superfamily	SUPERFAMILY	53098	Ribonuclease H-like	coord: 19..103

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS015493.1	MS015493.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0004523	RNA-DNA hybrid ribonuclease activity