MS015416 (gene) Bitter gourd (TR) v1

Overview


Name	MS015416
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Ribonuclease H-like superfamily protein
Location	scaffold586: 197109 .. 197519 (-)
RNA-Seq Expression	MS015416
Synteny	MS015416

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

CCTCCTCCCCTCCATACCTGGGCACTGAACGTTGATGCTTCTTGGTCGGCAGTTCATCAGATGGGCGGTCTCGGCTGGGTACTTCGGAACTGGGAGGGCGCTATTATTCTTGCGGGGAACAAATCAGTGGATCACGGTCCTAGTAGCATCAAGGCATTGGAAGCTATGGCCGTTCTTAAAGGGCTTCGGGCGATTAACTATCTTGTGACTTTTCCAATCCGAGTGGAATCTGATTCTGCGGAGGTAGTATCGCTCTTGAGAGGAGAAGATACCGATTTGACAGAAATTTTCTGGGTTGTTAATGAAATTCGTTATTGGCAAGATTCGGGTGGGGTTTGTGATTTTGTAAAGGTTGATCGGATTGCGAATTACTGTGCCCATCACCTTGCGCGTCGTGCTTCAGAGAGTAGG

mRNA sequence

CCTCCTCCCCTCCATACCTGGGCACTGAACGTTGATGCTTCTTGGTCGGCAGTTCATCAGATGGGCGGTCTCGGCTGGGTACTTCGGAACTGGGAGGGCGCTATTATTCTTGCGGGGAACAAATCAGTGGATCACGGTCCTAGTAGCATCAAGGCATTGGAAGCTATGGCCGTTCTTAAAGGGCTTCGGGCGATTAACTATCTTGTGACTTTTCCAATCCGAGTGGAATCTGATTCTGCGGAGGTAGTATCGCTCTTGAGAGGAGAAGATACCGATTTGACAGAAATTTTCTGGGTTGTTAATGAAATTCGTTATTGGCAAGATTCGGGTGGGGTTTGTGATTTTGTAAAGGTTGATCGGATTGCGAATTACTGTGCCCATCACCTTGCGCGTCGTGCTTCAGAGAGTAGG

Coding sequence (CDS)

CCTCCTCCCCTCCATACCTGGGCACTGAACGTTGATGCTTCTTGGTCGGCAGTTCATCAGATGGGCGGTCTCGGCTGGGTACTTCGGAACTGGGAGGGCGCTATTATTCTTGCGGGGAACAAATCAGTGGATCACGGTCCTAGTAGCATCAAGGCATTGGAAGCTATGGCCGTTCTTAAAGGGCTTCGGGCGATTAACTATCTTGTGACTTTTCCAATCCGAGTGGAATCTGATTCTGCGGAGGTAGTATCGCTCTTGAGAGGAGAAGATACCGATTTGACAGAAATTTTCTGGGTTGTTAATGAAATTCGTTATTGGCAAGATTCGGGTGGGGTTTGTGATTTTGTAAAGGTTGATCGGATTGCGAATTACTGTGCCCATCACCTTGCGCGTCGTGCTTCAGAGAGTAGG

Protein sequence

PPPLHTWALNVDASWSAVHQMGGLGWVLRNWEGAIILAGNKSVDHGPSSIKALEAMAVLKGLRAINYLVTFPIRVESDSAEVVSLLRGEDTDLTEIFWVVNEIRYWQDSGGVCDFVKVDRIANYCAHHLARRASESR

Homology

[Close]

BLAST of MS015416 vs. NCBI nr
Match: XP_022155262.1 (uncharacterized protein LOC111022403 [Momordica charantia])

HSP 1 Score: 137.1 bits (344), Expect = 1.1e-28
Identity = 70/138 (50.72%), Postives = 93/138 (67.39%), Query Frame = 0


Query: 1   PPPLHTWALNVDASWSAVHQMGGLGWVLRNWEGAIILAGNKSVDHGPSSIKALEAMAVLK 60
           PPP+H W LN DASWS     GG+GW++R+W+G I+LAGN+ V+   +++K LEA A+L+
Sbjct: 73  PPPMHIWTLNADASWSDSTHRGGIGWIIRSWDGDIVLAGNRFVE-ACNNVKLLEASAILE 132

Query: 61  GLRAINYL-VTFPIRVESDSAEVVSLLRGEDTDLTEIFWVVNEIRYWQDSGGVCDFVKVD 120
           GLR +  L V  P+ +E+DSAEV SLL  +  DLT+  WVV EI   +DS  +  F KV 
Sbjct: 133 GLRNLTNLGVLRPLHIETDSAEVESLLNRKREDLTKTGWVVEEILNLRDSCEILAFAKVG 192

Query: 121 RIANYCAHHLARRASESR 138
           R  N CAH LA+RAS  R
Sbjct: 193 RETNGCAHSLAQRASVLR 209

[Close]

BLAST of MS015416 vs. NCBI nr
Match: XP_022143535.1 (uncharacterized protein LOC111013412 [Momordica charantia])

HSP 1 Score: 100.1 bits (248), Expect = 1.5e-17
Identity = 54/136 (39.71%), Postives = 76/136 (55.88%), Query Frame = 0


Query: 1   PPPLHTWALNVDASWSAVHQMGGLGWVLRNWEGAIILAGNKSVDHGPSSIKALEAMAVLK 60
           PP  ++W LN +A+W A    GG+GW+LR+ +G +I A  + +     +I  LE MA+ +
Sbjct: 81  PPTSNSWKLNTNAAWRADTNTGGIGWILRDEKGEVIKASCRII-RAERNITYLEVMAICE 140

Query: 61  GLRAINYLVTFPIRVESDSAEVVSLLRGEDTDLTEIFWVVNEIRYWQDSGGVCDFVKVDR 120
           GLRAI      PI +ESDS E + LL  +  D TEI W++ EI        +     + R
Sbjct: 141 GLRAIRQEHCRPIHLESDSLEAIHLLHRQCQDQTEIIWLLEEIWQMMKDMEIVSMRHISR 200

Query: 121 IANYCAHHLARRASES 137
            AN  AH LARRA E+
Sbjct: 201 EANKVAHGLARRAMEN 215

[Close]

BLAST of MS015416 vs. NCBI nr
Match: XP_022156777.1 (uncharacterized protein LOC111023608 [Momordica charantia])

HSP 1 Score: 95.5 bits (236), Expect = 3.8e-16
Identity = 55/144 (38.19%), Postives = 78/144 (54.17%), Query Frame = 0


Query: 1   PPPLHTWALNVDASWSAVHQMGGLGWVLRNWEGAIILAGNKSVDHGPSSIKALEAMAVLK 60
           PP  ++W LN DA+W A    GG+GW+LR+ +G +I A  + +     +I  LE MA+ +
Sbjct: 81  PPTSNSWKLNTDAAWRADTNTGGIGWILRDEKGEVIKADCRII-RTERNITYLEVMAICE 140

Query: 61  GLRAINYLVTFPIR--------VESDSAEVVSLLRGEDTDLTEIFWVVNEIRYWQDSGGV 120
           GLRAI      PI+        +ESDS E + LL  +  D TEI W++ EI    +   +
Sbjct: 141 GLRAIRQEHCRPIQQEHCRPIHLESDSLEAIHLLHRQCQDQTEIIWLLEEIWQMMEDMKI 200

Query: 121 CDFVKVDRIANYCAHHLARRASES 137
                + R AN  AH LARRA E+
Sbjct: 201 VSMRHISREANKVAHDLARRAMEN 223

[Close]

BLAST of MS015416 vs. NCBI nr
Match: XP_022143317.1 (uncharacterized protein LOC111013216 [Momordica charantia])

HSP 1 Score: 92.8 bits (229), Expect = 2.4e-15
Identity = 44/93 (47.31%), Postives = 60/93 (64.52%), Query Frame = 0


Query: 2   PPLHTWALNVDASWSAVHQMGGLGWVLRNWEGAIILAGNKSVDHGPSSIKALEAMAVLKG 61
           PP + W LN DASWS   ++GG+GW+L +  G I+LAGN  +      I ALE M +++G
Sbjct: 108 PPTNCWKLNTDASWSEEREVGGIGWILCDCRGEIVLAGNCKI-REKKEINALELMTIIRG 167

Query: 62  LRAINYLVTFPIRVESDSAEVVSLLRGEDTDLT 95
           L+ IN     PI +ESDS EV+ L++ ED DLT
Sbjct: 168 LQFINMQSRSPIYLESDSVEVIRLMKKEDVDLT 199

[Close]

BLAST of MS015416 vs. NCBI nr
Match: XP_022148737.1 (uncharacterized protein LOC111017329 [Momordica charantia])

HSP 1 Score: 87.0 bits (214), Expect = 1.3e-13
Identity = 45/133 (33.83%), Postives = 75/133 (56.39%), Query Frame = 0


Query: 3   PLHTWALNVDASWSAVHQMGGLGWVLRNWEGAIILAGNKSVDHGPSSIKALEAMAVLKGL 62
           P + W LN DA+W A   +GGLGW++RN +  I  AG +++      I  LE MA+  G+
Sbjct: 9   PDNFWKLNTDAAWHANQGVGGLGWIIRNEKREIKKAGGQTITDA-RDITYLELMAIWLGI 68

Query: 63  RAINYLVTFPIRVESDSAEVVSLLRGEDTDLTEIFWVVNEIRYWQDSGGVCDFVKVDRIA 122
            A+    +  + +ES+S E + L++G   ++TEI W+V +I+ + +   +  F  V R  
Sbjct: 69  EAVVSSSSVSLIIESESLEAIHLIQGIQQNVTEILWLVQDIKNFGERARLKRFQHVQRDG 128

Query: 123 NYCAHHLARRASE 136
           N  A  +A RA++
Sbjct: 129 NSVAQEIANRAAD 140

[Close]

BLAST of MS015416 vs. ExPASy TrEMBL
Match: A0A6J1DNV9 (uncharacterized protein LOC111022403 OS=Momordica charantia OX=3673 GN=LOC111022403 PE=4 SV=1)

HSP 1 Score: 137.1 bits (344), Expect = 5.5e-29
Identity = 70/138 (50.72%), Postives = 93/138 (67.39%), Query Frame = 0


Query: 1   PPPLHTWALNVDASWSAVHQMGGLGWVLRNWEGAIILAGNKSVDHGPSSIKALEAMAVLK 60
           PPP+H W LN DASWS     GG+GW++R+W+G I+LAGN+ V+   +++K LEA A+L+
Sbjct: 73  PPPMHIWTLNADASWSDSTHRGGIGWIIRSWDGDIVLAGNRFVE-ACNNVKLLEASAILE 132

Query: 61  GLRAINYL-VTFPIRVESDSAEVVSLLRGEDTDLTEIFWVVNEIRYWQDSGGVCDFVKVD 120
           GLR +  L V  P+ +E+DSAEV SLL  +  DLT+  WVV EI   +DS  +  F KV 
Sbjct: 133 GLRNLTNLGVLRPLHIETDSAEVESLLNRKREDLTKTGWVVEEILNLRDSCEILAFAKVG 192

Query: 121 RIANYCAHHLARRASESR 138
           R  N CAH LA+RAS  R
Sbjct: 193 RETNGCAHSLAQRASVLR 209

[Close]

BLAST of MS015416 vs. ExPASy TrEMBL
Match: A0A6J1CP26 (uncharacterized protein LOC111013412 OS=Momordica charantia OX=3673 GN=LOC111013412 PE=4 SV=1)

HSP 1 Score: 100.1 bits (248), Expect = 7.4e-18
Identity = 54/136 (39.71%), Postives = 76/136 (55.88%), Query Frame = 0


Query: 1   PPPLHTWALNVDASWSAVHQMGGLGWVLRNWEGAIILAGNKSVDHGPSSIKALEAMAVLK 60
           PP  ++W LN +A+W A    GG+GW+LR+ +G +I A  + +     +I  LE MA+ +
Sbjct: 81  PPTSNSWKLNTNAAWRADTNTGGIGWILRDEKGEVIKASCRII-RAERNITYLEVMAICE 140

Query: 61  GLRAINYLVTFPIRVESDSAEVVSLLRGEDTDLTEIFWVVNEIRYWQDSGGVCDFVKVDR 120
           GLRAI      PI +ESDS E + LL  +  D TEI W++ EI        +     + R
Sbjct: 141 GLRAIRQEHCRPIHLESDSLEAIHLLHRQCQDQTEIIWLLEEIWQMMKDMEIVSMRHISR 200

Query: 121 IANYCAHHLARRASES 137
            AN  AH LARRA E+
Sbjct: 201 EANKVAHGLARRAMEN 215

[Close]

BLAST of MS015416 vs. ExPASy TrEMBL
Match: A0A6J1DSV1 (uncharacterized protein LOC111023608 OS=Momordica charantia OX=3673 GN=LOC111023608 PE=4 SV=1)

HSP 1 Score: 95.5 bits (236), Expect = 1.8e-16
Identity = 55/144 (38.19%), Postives = 78/144 (54.17%), Query Frame = 0


Query: 1   PPPLHTWALNVDASWSAVHQMGGLGWVLRNWEGAIILAGNKSVDHGPSSIKALEAMAVLK 60
           PP  ++W LN DA+W A    GG+GW+LR+ +G +I A  + +     +I  LE MA+ +
Sbjct: 81  PPTSNSWKLNTDAAWRADTNTGGIGWILRDEKGEVIKADCRII-RTERNITYLEVMAICE 140

Query: 61  GLRAINYLVTFPIR--------VESDSAEVVSLLRGEDTDLTEIFWVVNEIRYWQDSGGV 120
           GLRAI      PI+        +ESDS E + LL  +  D TEI W++ EI    +   +
Sbjct: 141 GLRAIRQEHCRPIQQEHCRPIHLESDSLEAIHLLHRQCQDQTEIIWLLEEIWQMMEDMKI 200

Query: 121 CDFVKVDRIANYCAHHLARRASES 137
                + R AN  AH LARRA E+
Sbjct: 201 VSMRHISREANKVAHDLARRAMEN 223

[Close]

BLAST of MS015416 vs. ExPASy TrEMBL
Match: A0A6J1CQG0 (uncharacterized protein LOC111013216 OS=Momordica charantia OX=3673 GN=LOC111013216 PE=4 SV=1)

HSP 1 Score: 92.8 bits (229), Expect = 1.2e-15
Identity = 44/93 (47.31%), Postives = 60/93 (64.52%), Query Frame = 0


Query: 2   PPLHTWALNVDASWSAVHQMGGLGWVLRNWEGAIILAGNKSVDHGPSSIKALEAMAVLKG 61
           PP + W LN DASWS   ++GG+GW+L +  G I+LAGN  +      I ALE M +++G
Sbjct: 108 PPTNCWKLNTDASWSEEREVGGIGWILCDCRGEIVLAGNCKI-REKKEINALELMTIIRG 167

Query: 62  LRAINYLVTFPIRVESDSAEVVSLLRGEDTDLT 95
           L+ IN     PI +ESDS EV+ L++ ED DLT
Sbjct: 168 LQFINMQSRSPIYLESDSVEVIRLMKKEDVDLT 199

[Close]

BLAST of MS015416 vs. ExPASy TrEMBL
Match: A0A6J1D5W1 (uncharacterized protein LOC111017329 OS=Momordica charantia OX=3673 GN=LOC111017329 PE=4 SV=1)

HSP 1 Score: 87.0 bits (214), Expect = 6.5e-14
Identity = 45/133 (33.83%), Postives = 75/133 (56.39%), Query Frame = 0


Query: 3   PLHTWALNVDASWSAVHQMGGLGWVLRNWEGAIILAGNKSVDHGPSSIKALEAMAVLKGL 62
           P + W LN DA+W A   +GGLGW++RN +  I  AG +++      I  LE MA+  G+
Sbjct: 9   PDNFWKLNTDAAWHANQGVGGLGWIIRNEKREIKKAGGQTITDA-RDITYLELMAIWLGI 68

Query: 63  RAINYLVTFPIRVESDSAEVVSLLRGEDTDLTEIFWVVNEIRYWQDSGGVCDFVKVDRIA 122
            A+    +  + +ES+S E + L++G   ++TEI W+V +I+ + +   +  F  V R  
Sbjct: 69  EAVVSSSSVSLIIESESLEAIHLIQGIQQNVTEILWLVQDIKNFGERARLKRFQHVQRDG 128

Query: 123 NYCAHHLARRASE 136
           N  A  +A RA++
Sbjct: 129 NSVAQEIANRAAD 140

[Close]

BLAST of MS015416 vs. TAIR 10
Match: AT2G02650.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 52.0 bits (123), Expect = 4.4e-07
Identity = 40/131 (30.53%), Postives = 59/131 (45.04%), Query Frame = 0


Query: 1   PPPLHTWALNVDASWSAVHQMGGLGWVLRNWEGAIILAGNKSVDHGPSSIKALEAMAVLK 60
           PPP      N D+ ++        GW +R   G I+L GN  +     S+ A EA+  L 
Sbjct: 208 PPPEGWVKCNFDSGYTQGSPYTRSGWTIRECNGHIVLCGNAKLQSSTCSLHA-EALGFLH 267

Query: 61  GLRAINYLVTFPIRVESDSAEVVSLL-RGEDTDLTEIFWVVNEIRYWQDSGGVCDFVKVD 120
            L+ I       +  ESDS  +V+L+  GED  L  +  ++ +IR+W      C    V+
Sbjct: 268 ALQVIWAHGLRYVWFESDSKSLVTLINNGEDHSL--LGTLIYDIRHWMLKLPYCSLEFVN 327

Query: 121 RIANYCAHHLA 131
           R  N  A  LA
Sbjct: 328 RERNSAADALA 335

[Close]

BLAST of MS015416 vs. TAIR 10
Match: AT4G29090.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 51.6 bits (122), Expect = 5.8e-07
Identity = 44/146 (30.14%), Postives = 69/146 (47.26%), Query Frame = 0


Query: 2   PPLHTWA-LNVDASWSAVHQMGGLGWVLRNWEGAIILAGNKSVDHGPSSIKA-LEAM--A 61
           PP H W   N DA+W+  ++  G+GWVLRN +G +   G +++    S ++A LEAM  A
Sbjct: 422 PPPHQWVKCNTDATWNRDNERCGIGWVLRNEKGEVKWMGARALPKLKSVLEAELEAMRWA 481

Query: 62  VLKGLR-AINYLVTFPIRVESDSAEVVSLLRGED---------TDLTEIFWVVNEIRYWQ 121
           VL   R   NY++      ESDS  ++ +L  ++          DL  +     E++   
Sbjct: 482 VLSLSRFQYNYVI-----FESDSQVLIEILNNDEIWPSLKPTIQDLQRLLSQFTEVK--- 541

Query: 122 DSGGVCDFVKVDRIANYCAHHLARRA 134
                  FV + R  N  A  +AR +
Sbjct: 542 -------FVFIPREGNTLAERVARES 552

[Close]

BLAST of MS015416 vs. TAIR 10
Match: AT1G52990.1 (thioredoxin family protein )

HSP 1 Score: 47.0 bits (110), Expect = 1.4e-05
Identity = 32/127 (25.20%), Postives = 60/127 (47.24%), Query Frame = 0


Query: 10  NVDASWSAVHQMGGLGWVLRNWEGAIILAGNKSVDHGPSSIKALEAMAVLKGLRAINYLV 69
           N DAS      + GLGW++RN +G ++  G      G  + +  E  A++  ++A +   
Sbjct: 51  NYDASHHEGDVVSGLGWLIRNSQGTVLECGMGKF-QGRMTPEEAECSALIWAIQATSAFG 110

Query: 70  TFPIRVESDSAEVVSLLRGEDTDLTEIFWVVNEIRYWQDSGGVCDFVKVDRIANYCAHHL 129
              +  E D++ V  L+  + +D   +   ++ I+ W  S    +F+   R  N CA  L
Sbjct: 111 YTKVIFEGDNSNVNRLINTK-SDNPRLKHYLDTIKSWIPSFTSTEFIFTHREQNQCADTL 170

Query: 130 ARRASES 137
            ++A +S
Sbjct: 171 VKKAIKS 175

The following BLAST results are available for this feature:

BLAST of MS015416 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022155262.1	1.1e-28	50.72	uncharacterized protein LOC111022403 [Momordica charantia]	[more]
XP_022143535.1	1.5e-17	39.71	uncharacterized protein LOC111013412 [Momordica charantia]	[more]
XP_022156777.1	3.8e-16	38.19	uncharacterized protein LOC111023608 [Momordica charantia]	[more]
XP_022143317.1	2.4e-15	47.31	uncharacterized protein LOC111013216 [Momordica charantia]	[more]
XP_022148737.1	1.3e-13	33.83	uncharacterized protein LOC111017329 [Momordica charantia]	[more]

BLAST of MS015416 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of MS015416 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DNV9	5.5e-29	50.72	uncharacterized protein LOC111022403 OS=Momordica charantia OX=3673 GN=LOC111022...	[more]
A0A6J1CP26	7.4e-18	39.71	uncharacterized protein LOC111013412 OS=Momordica charantia OX=3673 GN=LOC111013...	[more]
A0A6J1DSV1	1.8e-16	38.19	uncharacterized protein LOC111023608 OS=Momordica charantia OX=3673 GN=LOC111023...	[more]
A0A6J1CQG0	1.2e-15	47.31	uncharacterized protein LOC111013216 OS=Momordica charantia OX=3673 GN=LOC111013...	[more]
A0A6J1D5W1	6.5e-14	33.83	uncharacterized protein LOC111017329 OS=Momordica charantia OX=3673 GN=LOC111017...	[more]

BLAST of MS015416 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G02650.1	4.4e-07	30.53	Ribonuclease H-like superfamily protein	[more]
AT4G29090.1	5.8e-07	30.14	Ribonuclease H-like superfamily protein	[more]
AT1G52990.1	1.4e-05	25.20	thioredoxin family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036397	Ribonuclease H superfamily	GENE3D	3.30.420.10		coord: 7..137 e-value: 1.1E-12 score: 50.1
IPR002156	Ribonuclease H domain	PFAM	PF13456	RVT_3	coord: 10..133 e-value: 2.7E-20 score: 72.4
None	No IPR available	PANTHER	PTHR47723	OS05G0353850 PROTEIN	coord: 1..134
IPR044730	Ribonuclease H-like domain, plant type	CDD	cd06222	RNase_H_like	coord: 9..131 e-value: 6.55803E-18 score: 71.9616
IPR012337	Ribonuclease H-like superfamily	SUPERFAMILY	53098	Ribonuclease H-like	coord: 6..135

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS015416.1	MS015416.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0004523	RNA-DNA hybrid ribonuclease activity