MS015407 (gene) Bitter gourd (TR) v1

Overview


Name	MS015407
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	AMP dependent ligase
Location	scaffold586: 141891 .. 143615 (-)
RNA-Seq Expression	MS015407
Synteny	MS015407

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

CCCCATTTGCAGAGAGAGAGAAACAGAGGAAATCGAAATAACAAATTATCAGTTACCAATTCCATGGAAGATCTGAAGCCAAGCCCTGCAAATTCCTCTCCTCTTACGCCAATTGGCTTCCTCGACAGAGCCGCCACCGTCTACGGCGACTGCCCTTCAGTCATCTACGGCTCCACTGCCTACACGTGGTCCCAGACCCACCGTCGATGTCTTCAACTCGCTTCTTCCCTCTCATCCAACGGCATCCACAGGGGCCACGTCGTCTCCGTCGTCGCTCCCAACACCCCCGCCATGTACGAGCTCCAGTTCGCCGTTCCGATGGCCGGCGCCGTCCTCAACAACATCAATCTCCGCCTCGACGCTCGTACCATCTCTATCCTCCTCCGCCATAGCGAATCCAAGCTCGTCTTCGTCGATCAAGCCTCTTGCTCCCTTATCTCCGACGCCTTGGCCTTGTTTCCGCCGGAAGCCAGACGGCCGGCGCTCGTCCTGATCGCCGATGACGCTGCGGCGGAGGAGTCGTCTTCTTCACCGGTTTCCGGAGATTTCGTCGACACGTATGAGGGAATGGTGGAGAAAGGGAATCCAGAGTTCCGGTGGATTCGGCCGGCGAGTGATTGGGATCCGATTGTTTTGAATTACACTTCCGGAACGACGTCGTCGCCGAAAGGAGTGGTCCATTGCCACAGAGGAGTTTTCATGGTAGCTCTGGATTCGCTTCTAGAATGGGGCGTCCCTAAGCAATCAGTTTATTTATGGACTTTGCCGATCTTCCACGCCAATGGATGGTGCTTCCCGTGGGGAATCGCCGCCATAGGCGGCACAAATGTCTGTCTCAGAAAATTCGACGCGCCATTGATTTTTCGCCTAATTCATCGGCACAACGTGACGCACATGTGCGGAGCTCCAGTGGTGCTCAATATGCTCACGAACTCCCCGAAGAATAAGCCGCTCGATCGGCCAGTGAAGATCCTCACCGCCGGCGCGCCGCCGCCTGCAGCGGTTCTGTTCCGCACCGAATCGTTGGGTTTCGATGTGAGTCATGGGTACGGACTAACAGAGACCGCCGGGCTGGTGGTGGTGTGCTCGTGGAAGCCGCAGTGGAAGCGGCTACCGGCGGCGGAGAGAGCGCGGTTGAAGGCGCGACAGGGGGTGAGAACGATGGCAATAACAGAGGCGGACGTTGTGGATCCGGAGACTGGCAAGAGCGTGAAACGCGACGGATCGTCAATAGGAGAGATCGTTCTGAAAGGTGGATCGATCATGCTCGGTTACCTCAAAGATCCGGAAGCGACATCCAAATCGATGATCGAGGAGGGGTGGTTCTTCACAGGAGACGTCGGCGTGATGCACGGCGACGGGTATTTGGAGATCAAGGACCGATCGAAGGACGTGATCATAAGCGGAGGAGAGAACCTGAGCAGCGTGGAGGTAGAATCGGTACTGTACACAAATCCGGCGGTGAACGAGGCGGCGGTGGTGGCGCGACCGGATGAGTTCTGGGGGGAGACGCCGTGCGCGTTCGTGAGCTTGAGAGAAGGATTGAGTCGGAGGCCGACGGAGGAAGAGATAATTGAGTACTGCAGAGGGAAGCTGCCGCGGTTTATGGTGCCGAAGACGGTGGTTTTCAAGGCAGAACTGCCGAAGACGTCGACGGGGAAGATCAAGAAGTTCGTGCTTAGGGAGATTGCCAAGTCGATGGGCCCTGCACGGGCGAGTCGCTTG

mRNA sequence

CCCCATTTGCAGAGAGAGAGAAACAGAGGAAATCGAAATAACAAATTATCAGTTACCAATTCCATGGAAGATCTGAAGCCAAGCCCTGCAAATTCCTCTCCTCTTACGCCAATTGGCTTCCTCGACAGAGCCGCCACCGTCTACGGCGACTGCCCTTCAGTCATCTACGGCTCCACTGCCTACACGTGGTCCCAGACCCACCGTCGATGTCTTCAACTCGCTTCTTCCCTCTCATCCAACGGCATCCACAGGGGCCACGTCGTCTCCGTCGTCGCTCCCAACACCCCCGCCATGTACGAGCTCCAGTTCGCCGTTCCGATGGCCGGCGCCGTCCTCAACAACATCAATCTCCGCCTCGACGCTCGTACCATCTCTATCCTCCTCCGCCATAGCGAATCCAAGCTCGTCTTCGTCGATCAAGCCTCTTGCTCCCTTATCTCCGACGCCTTGGCCTTGTTTCCGCCGGAAGCCAGACGGCCGGCGCTCGTCCTGATCGCCGATGACGCTGCGGCGGAGGAGTCGTCTTCTTCACCGGTTTCCGGAGATTTCGTCGACACGTATGAGGGAATGGTGGAGAAAGGGAATCCAGAGTTCCGGTGGATTCGGCCGGCGAGTGATTGGGATCCGATTGTTTTGAATTACACTTCCGGAACGACGTCGTCGCCGAAAGGAGTGGTCCATTGCCACAGAGGAGTTTTCATGGTAGCTCTGGATTCGCTTCTAGAATGGGGCGTCCCTAAGCAATCAGTTTATTTATGGACTTTGCCGATCTTCCACGCCAATGGATGGTGCTTCCCGTGGGGAATCGCCGCCATAGGCGGCACAAATGTCTGTCTCAGAAAATTCGACGCGCCATTGATTTTTCGCCTAATTCATCGGCACAACGTGACGCACATGTGCGGAGCTCCAGTGGTGCTCAATATGCTCACGAACTCCCCGAAGAATAAGCCGCTCGATCGGCCAGTGAAGATCCTCACCGCCGGCGCGCCGCCGCCTGCAGCGGTTCTGTTCCGCACCGAATCGTTGGGTTTCGATGTGAGTCATGGGTACGGACTAACAGAGACCGCCGGGCTGGTGGTGGTGTGCTCGTGGAAGCCGCAGTGGAAGCGGCTACCGGCGGCGGAGAGAGCGCGGTTGAAGGCGCGACAGGGGGTGAGAACGATGGCAATAACAGAGGCGGACGTTGTGGATCCGGAGACTGGCAAGAGCGTGAAACGCGACGGATCGTCAATAGGAGAGATCGTTCTGAAAGGTGGATCGATCATGCTCGGTTACCTCAAAGATCCGGAAGCGACATCCAAATCGATGATCGAGGAGGGGTGGTTCTTCACAGGAGACGTCGGCGTGATGCACGGCGACGGGTATTTGGAGATCAAGGACCGATCGAAGGACGTGATCATAAGCGGAGGAGAGAACCTGAGCAGCGTGGAGGTAGAATCGGTACTGTACACAAATCCGGCGGTGAACGAGGCGGCGGTGGTGGCGCGACCGGATGAGTTCTGGGGGGAGACGCCGTGCGCGTTCGTGAGCTTGAGAGAAGGATTGAGTCGGAGGCCGACGGAGGAAGAGATAATTGAGTACTGCAGAGGGAAGCTGCCGCGGTTTATGGTGCCGAAGACGGTGGTTTTCAAGGCAGAACTGCCGAAGACGTCGACGGGGAAGATCAAGAAGTTCGTGCTTAGGGAGATTGCCAAGTCGATGGGCCCTGCACGGGCGAGTCGCTTG

Coding sequence (CDS)

CCCCATTTGCAGAGAGAGAGAAACAGAGGAAATCGAAATAACAAATTATCAGTTACCAATTCCATGGAAGATCTGAAGCCAAGCCCTGCAAATTCCTCTCCTCTTACGCCAATTGGCTTCCTCGACAGAGCCGCCACCGTCTACGGCGACTGCCCTTCAGTCATCTACGGCTCCACTGCCTACACGTGGTCCCAGACCCACCGTCGATGTCTTCAACTCGCTTCTTCCCTCTCATCCAACGGCATCCACAGGGGCCACGTCGTCTCCGTCGTCGCTCCCAACACCCCCGCCATGTACGAGCTCCAGTTCGCCGTTCCGATGGCCGGCGCCGTCCTCAACAACATCAATCTCCGCCTCGACGCTCGTACCATCTCTATCCTCCTCCGCCATAGCGAATCCAAGCTCGTCTTCGTCGATCAAGCCTCTTGCTCCCTTATCTCCGACGCCTTGGCCTTGTTTCCGCCGGAAGCCAGACGGCCGGCGCTCGTCCTGATCGCCGATGACGCTGCGGCGGAGGAGTCGTCTTCTTCACCGGTTTCCGGAGATTTCGTCGACACGTATGAGGGAATGGTGGAGAAAGGGAATCCAGAGTTCCGGTGGATTCGGCCGGCGAGTGATTGGGATCCGATTGTTTTGAATTACACTTCCGGAACGACGTCGTCGCCGAAAGGAGTGGTCCATTGCCACAGAGGAGTTTTCATGGTAGCTCTGGATTCGCTTCTAGAATGGGGCGTCCCTAAGCAATCAGTTTATTTATGGACTTTGCCGATCTTCCACGCCAATGGATGGTGCTTCCCGTGGGGAATCGCCGCCATAGGCGGCACAAATGTCTGTCTCAGAAAATTCGACGCGCCATTGATTTTTCGCCTAATTCATCGGCACAACGTGACGCACATGTGCGGAGCTCCAGTGGTGCTCAATATGCTCACGAACTCCCCGAAGAATAAGCCGCTCGATCGGCCAGTGAAGATCCTCACCGCCGGCGCGCCGCCGCCTGCAGCGGTTCTGTTCCGCACCGAATCGTTGGGTTTCGATGTGAGTCATGGGTACGGACTAACAGAGACCGCCGGGCTGGTGGTGGTGTGCTCGTGGAAGCCGCAGTGGAAGCGGCTACCGGCGGCGGAGAGAGCGCGGTTGAAGGCGCGACAGGGGGTGAGAACGATGGCAATAACAGAGGCGGACGTTGTGGATCCGGAGACTGGCAAGAGCGTGAAACGCGACGGATCGTCAATAGGAGAGATCGTTCTGAAAGGTGGATCGATCATGCTCGGTTACCTCAAAGATCCGGAAGCGACATCCAAATCGATGATCGAGGAGGGGTGGTTCTTCACAGGAGACGTCGGCGTGATGCACGGCGACGGGTATTTGGAGATCAAGGACCGATCGAAGGACGTGATCATAAGCGGAGGAGAGAACCTGAGCAGCGTGGAGGTAGAATCGGTACTGTACACAAATCCGGCGGTGAACGAGGCGGCGGTGGTGGCGCGACCGGATGAGTTCTGGGGGGAGACGCCGTGCGCGTTCGTGAGCTTGAGAGAAGGATTGAGTCGGAGGCCGACGGAGGAAGAGATAATTGAGTACTGCAGAGGGAAGCTGCCGCGGTTTATGGTGCCGAAGACGGTGGTTTTCAAGGCAGAACTGCCGAAGACGTCGACGGGGAAGATCAAGAAGTTCGTGCTTAGGGAGATTGCCAAGTCGATGGGCCCTGCACGGGCGAGTCGCTTG

Protein sequence

PHLQRERNRGNRNNKLSVTNSMEDLKPSPANSSPLTPIGFLDRAATVYGDCPSVIYGSTAYTWSQTHRRCLQLASSLSSNGIHRGHVVSVVAPNTPAMYELQFAVPMAGAVLNNINLRLDARTISILLRHSESKLVFVDQASCSLISDALALFPPEARRPALVLIADDAAAEESSSSPVSGDFVDTYEGMVEKGNPEFRWIRPASDWDPIVLNYTSGTTSSPKGVVHCHRGVFMVALDSLLEWGVPKQSVYLWTLPIFHANGWCFPWGIAAIGGTNVCLRKFDAPLIFRLIHRHNVTHMCGAPVVLNMLTNSPKNKPLDRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETAGLVVVCSWKPQWKRLPAAERARLKARQGVRTMAITEADVVDPETGKSVKRDGSSIGEIVLKGGSIMLGYLKDPEATSKSMIEEGWFFTGDVGVMHGDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPDEFWGETPCAFVSLREGLSRRPTEEEIIEYCRGKLPRFMVPKTVVFKAELPKTSTGKIKKFVLREIAKSMGPARASRL

Homology

[Close]

BLAST of MS015407 vs. NCBI nr
Match: XP_022939278.1 (probable acyl-activating enzyme 5, peroxisomal [Cucurbita moschata] >KAG6579057.1 putative acyl-activating enzyme 5, peroxisomal, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 963.0 bits (2488), Expect = 1.2e-276
Identity = 468/557 (84.02%), Postives = 511/557 (91.74%), Query Frame = 0


Query: 22  MEDLKPSPANSSPLTPIGFLDRAATVYGDCPSVIYGSTAYTWSQTHRRCLQLASSLSSNG 81
           MEDLKP+PANSSPLTPIGFL+RAA VYGDCPS+IY ST YTW++THRRCLQLASSL S G
Sbjct: 1   MEDLKPNPANSSPLTPIGFLERAAVVYGDCPSIIYNSTTYTWTETHRRCLQLASSLVSIG 60

Query: 82  IHRGHVVSVVAPNTPAMYELQFAVPMAGAVLNNINLRLDARTISILLRHSESKLVFVDQA 141
           I RG VVSVV+PNT AMYEL FAVPMAGAVLN INLRLDARTIS++LRH E+ L+FVDQA
Sbjct: 61  IQRGQVVSVVSPNTAAMYELHFAVPMAGAVLNTINLRLDARTISVILRHCETNLIFVDQA 120

Query: 142 SCSLISDALALFPPEARRPALVLIADDAAAEESSSSPV---SGDFVDTYEGMVEKGNPEF 201
           SC LISDALALFPPE +RP L+LIAD+AAAE+SSS+       DFVD+YE MV+KG+P F
Sbjct: 121 SCGLISDALALFPPEMKRPPLILIADNAAAEDSSSASSVWNRADFVDSYEEMVQKGDPLF 180

Query: 202 RWIRPASDWDPIVLNYTSGTTSSPKGVVHCHRGVFMVALDSLLEWGVPKQSVYLWTLPIF 261
           +WIRPAS+WDPIVLNYTSGTTSSPKGVVHCHRG+F V LDSL+EWGVPK+SVYLWTLP+F
Sbjct: 181 QWIRPASEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVTLDSLIEWGVPKESVYLWTLPMF 240

Query: 262 HANGWCFPWGIAAIGGTNVCLRKFDAPLIFRLIHRHNVTHMCGAPVVLNMLTNSPKNKPL 321
           HANGW FPWGIAA+GGTNVC+RKFDA  IF LIHR++VTH+CGAPVVLNMLTNSP N+PL
Sbjct: 241 HANGWSFPWGIAAVGGTNVCIRKFDAAAIFALIHRYHVTHLCGAPVVLNMLTNSPDNRPL 300

Query: 322 DRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETAGLVVVCSWKPQWKRLPAAERAR 381
           DRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTET GLV+ CSWK  W RLPAAERAR
Sbjct: 301 DRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETGGLVICCSWKSGWNRLPAAERAR 360

Query: 382 LKARQGVRTMAITEADVVDPETGKSVKRDGSSIGEIVLKGGSIMLGYLKDPEATSKSMIE 441
           LKARQGVRT+A+  ADV+DPETGKSVKRDG+SIGEIVL+G S+MLGYLKDPEAT+K+M  
Sbjct: 361 LKARQGVRTLAMAVADVLDPETGKSVKRDGTSIGEIVLRGSSMMLGYLKDPEATAKAMTA 420

Query: 442 EGWFFTGDVGVMHGDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPD 501
           EGWFFTGDVGVMH DGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPD
Sbjct: 421 EGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPD 480

Query: 502 EFWGETPCAFVSLREGLSRRPTEEEIIEYCRGKLPRFMVPKTVVFKAELPKTSTGKIKKF 561
           EFWGETPCAFVSL+EGL+RRPTE EIIEYCRGKLPRFMVPKTVVF AELPKTSTGKI+KF
Sbjct: 481 EFWGETPCAFVSLKEGLTRRPTEGEIIEYCRGKLPRFMVPKTVVFTAELPKTSTGKIQKF 540

Query: 562 VLREIAKSMGPARASRL 576
           VLRE+AKSMGPA  SRL
Sbjct: 541 VLREMAKSMGPAGKSRL 557

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BLAST of MS015407 vs. NCBI nr
Match: XP_022993747.1 (probable acyl-activating enzyme 5, peroxisomal [Cucurbita maxima])

HSP 1 Score: 959.5 bits (2479), Expect = 1.3e-275
Identity = 468/561 (83.42%), Postives = 510/561 (90.91%), Query Frame = 0


Query: 22  MEDLKPSPANSSPLTPIGFLDRAATVYGDCPSVIYGSTAYTWSQTHRRCLQLASSLSSNG 81
           MEDLKP+PANSSPLTPIGFL+RAA VYGDCPS+IY ST YTW++THRRCLQLASSL S G
Sbjct: 1   MEDLKPNPANSSPLTPIGFLERAAVVYGDCPSIIYNSTTYTWTETHRRCLQLASSLVSIG 60

Query: 82  IHRGHVVSVVAPNTPAMYELQFAVPMAGAVLNNINLRLDARTISILLRHSESKLVFVDQA 141
           I RG VVSVV+PNTPAMYEL FAVPMAGAVLN INLRLD+RTIS++LRH E+ L+FVDQA
Sbjct: 61  IQRGQVVSVVSPNTPAMYELHFAVPMAGAVLNTINLRLDSRTISVILRHCETNLIFVDQA 120

Query: 142 SCSLISDALALFPPEARRPALVLIADDAAAEESSSSPVS-------GDFVDTYEGMVEKG 201
           SC LISDALALFPPE +RP L+LIAD+AAAE+SSSS  S        DFVD+YE MV+KG
Sbjct: 121 SCGLISDALALFPPEMKRPPLILIADNAAAEDSSSSSSSASSVSNRADFVDSYEEMVQKG 180

Query: 202 NPEFRWIRPASDWDPIVLNYTSGTTSSPKGVVHCHRGVFMVALDSLLEWGVPKQSVYLWT 261
           +P F+WIRP S+WDPIVLNYTSGTTSSPKGVVHCHRG+F V LDSL+EWGV KQSVYLWT
Sbjct: 181 DPLFQWIRPVSEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVTLDSLIEWGVSKQSVYLWT 240

Query: 262 LPIFHANGWCFPWGIAAIGGTNVCLRKFDAPLIFRLIHRHNVTHMCGAPVVLNMLTNSPK 321
           LP+FHANGW FPWGIAA+GGTNVC+RKFDA  IF LIHR++VTH+CGAPVVLNMLTNSP 
Sbjct: 241 LPMFHANGWSFPWGIAAVGGTNVCIRKFDAATIFALIHRYHVTHLCGAPVVLNMLTNSPD 300

Query: 322 NKPLDRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETAGLVVVCSWKPQWKRLPAA 381
           N+PLDRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTET GLV+ CSWK  W RLPAA
Sbjct: 301 NRPLDRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETGGLVICCSWKSGWNRLPAA 360

Query: 382 ERARLKARQGVRTMAITEADVVDPETGKSVKRDGSSIGEIVLKGGSIMLGYLKDPEATSK 441
           ERARLKARQGVRT+A+  ADV+ PETGKSVKRDG+SIGEIVL+G S+MLGYLKDPEAT+K
Sbjct: 361 ERARLKARQGVRTLAMAVADVLCPETGKSVKRDGTSIGEIVLRGSSMMLGYLKDPEATAK 420

Query: 442 SMIEEGWFFTGDVGVMHGDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVV 501
           +M  EGWFFTGDVGVMH DGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVV
Sbjct: 421 AMTAEGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVV 480

Query: 502 ARPDEFWGETPCAFVSLREGLSRRPTEEEIIEYCRGKLPRFMVPKTVVFKAELPKTSTGK 561
           ARPDEFWGETPCAFVSL+EGL+RRPTE EIIEYCRGKLPRFMVPKTVVF AELPKTSTGK
Sbjct: 481 ARPDEFWGETPCAFVSLKEGLTRRPTEGEIIEYCRGKLPRFMVPKTVVFMAELPKTSTGK 540

Query: 562 IKKFVLREIAKSMGPARASRL 576
           I+KFVLRE+AKSMGPA  SRL
Sbjct: 541 IQKFVLREMAKSMGPAGRSRL 561

[Close]

BLAST of MS015407 vs. NCBI nr
Match: XP_023550909.1 (probable acyl-activating enzyme 5, peroxisomal [Cucurbita pepo subsp. pepo])

HSP 1 Score: 959.5 bits (2479), Expect = 1.3e-275
Identity = 468/561 (83.42%), Postives = 511/561 (91.09%), Query Frame = 0


Query: 22  MEDLKPSPANSSPLTPIGFLDRAATVYGDCPSVIYGSTAYTWSQTHRRCLQLASSLSSNG 81
           MEDLKP+PANSSPLTPIGFL+RAA VYGDC S+IY ST YTW++THRRCLQLASSL S G
Sbjct: 1   MEDLKPNPANSSPLTPIGFLERAAVVYGDCRSIIYDSTTYTWTETHRRCLQLASSLVSIG 60

Query: 82  IHRGHVVSVVAPNTPAMYELQFAVPMAGAVLNNINLRLDARTISILLRHSESKLVFVDQA 141
           I RG VVSVV+PNT AMYEL FAVPMAGAVLN INLRLDARTIS++LRH E+ L+FVDQA
Sbjct: 61  IQRGQVVSVVSPNTAAMYELHFAVPMAGAVLNTINLRLDARTISVILRHCETNLIFVDQA 120

Query: 142 SCSLISDALALFPPEARRPALVLIADDAAAEESSSSPVS-------GDFVDTYEGMVEKG 201
           SC LISDALALFPPE +RP L+LIAD+AAAE+SSSS  S        DFVD+YE MV+KG
Sbjct: 121 SCGLISDALALFPPEMKRPPLILIADNAAAEDSSSSSSSASSVLNRADFVDSYEEMVQKG 180

Query: 202 NPEFRWIRPASDWDPIVLNYTSGTTSSPKGVVHCHRGVFMVALDSLLEWGVPKQSVYLWT 261
           +P F+WIRPAS+WDPIVLNYTSGTTSSPKGVVHCHRG+F V LDSL+EWGVPK+SVYLWT
Sbjct: 181 DPLFQWIRPASEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVTLDSLIEWGVPKESVYLWT 240

Query: 262 LPIFHANGWCFPWGIAAIGGTNVCLRKFDAPLIFRLIHRHNVTHMCGAPVVLNMLTNSPK 321
           LP+FHANGW FPWGIAA+GGTNVC+RKFDA  IF LIHR++VTH+CGAPVVLNMLTNSP 
Sbjct: 241 LPMFHANGWSFPWGIAAVGGTNVCIRKFDAAAIFALIHRYHVTHLCGAPVVLNMLTNSPD 300

Query: 322 NKPLDRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETAGLVVVCSWKPQWKRLPAA 381
           N+PLDRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTET GLV+ CSWK  W RLPAA
Sbjct: 301 NRPLDRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETGGLVICCSWKSGWNRLPAA 360

Query: 382 ERARLKARQGVRTMAITEADVVDPETGKSVKRDGSSIGEIVLKGGSIMLGYLKDPEATSK 441
           +RARLKARQGVRT+A+  ADV+DPETGKSVKRDG+SIGEIVL+G S+MLGYLKDPEAT+K
Sbjct: 361 DRARLKARQGVRTLAMAVADVLDPETGKSVKRDGTSIGEIVLRGSSMMLGYLKDPEATAK 420

Query: 442 SMIEEGWFFTGDVGVMHGDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVV 501
           +M  EGWFFTGDVGVMH DGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVV
Sbjct: 421 AMTAEGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVV 480

Query: 502 ARPDEFWGETPCAFVSLREGLSRRPTEEEIIEYCRGKLPRFMVPKTVVFKAELPKTSTGK 561
           ARPDEFWGETPCAFVSL+EGL+RRPTE EIIEYCRGKLPRFMVPKTVVF AELPKTSTGK
Sbjct: 481 ARPDEFWGETPCAFVSLKEGLTRRPTEGEIIEYCRGKLPRFMVPKTVVFTAELPKTSTGK 540

Query: 562 IKKFVLREIAKSMGPARASRL 576
           I+KFVLRE+AKSMGPA  SRL
Sbjct: 541 IQKFVLREMAKSMGPAGKSRL 561

[Close]

BLAST of MS015407 vs. NCBI nr
Match: XP_038877068.1 (probable acyl-activating enzyme 5, peroxisomal [Benincasa hispida])

HSP 1 Score: 950.7 bits (2456), Expect = 6.0e-273
Identity = 464/576 (80.56%), Postives = 519/576 (90.10%), Query Frame = 0


Query: 4   QRERNRGNRNNKLSVTNSMEDLKPSPANSSPLTPIGFLDRAATVYGDCPSVIYGSTAYTW 63
           + +++  NR   +S + SMEDLKPS ANSSPLTPIGFL+RA+ +YGD PS+IY ST YTW
Sbjct: 10  REKQSNHNRATTISQSISMEDLKPSLANSSPLTPIGFLERASVIYGDSPSIIYNSTTYTW 69

Query: 64  SQTHRRCLQLASSLSSNGIHRGHVVSVVAPNTPAMYELQFAVPMAGAVLNNINLRLDART 123
           S+T+RRCL++ASSLSS GI +G+VVSV+APNTP MYELQFAVPMAGAVLN INLRLDART
Sbjct: 70  SETYRRCLRVASSLSSIGIQKGYVVSVIAPNTPPMYELQFAVPMAGAVLNCINLRLDART 129

Query: 124 ISILLRHSESKLVFVDQASCSLISDALALFPPEARRPALVLIADDAAAEE----SSSSPV 183
           ISILLRH ESKL+F+DQASC LISDALALFPPE +RP LVLIADD A E+    SSSS +
Sbjct: 130 ISILLRHCESKLIFIDQASCGLISDALALFPPETKRPLLVLIADDTAVEDSLSSSSSSVI 189

Query: 184 SGDFVDTYEGMVEKGNPEFRWIRPASDWDPIVLNYTSGTTSSPKGVVHCHRGVFMVALDS 243
            G+FVD+YE MV+KG+ EFRWIRP S+WDPIV+NYTSGTTSSPKGVVH HRG+F V LDS
Sbjct: 190 GGEFVDSYEEMVQKGDDEFRWIRPVSEWDPIVMNYTSGTTSSPKGVVHSHRGIFAVTLDS 249

Query: 244 LLEWGVPKQSVYLWTLPIFHANGWCFPWGIAAIGGTNVCLRKFDAPLIFRLIHRHNVTHM 303
           L+EWGVPKQSVYLWTLP+FHANGW FP+GIAA+GGTNVC+RKFDA LIF LIHRH+VTH+
Sbjct: 250 LIEWGVPKQSVYLWTLPMFHANGWSFPFGIAAVGGTNVCIRKFDAALIFSLIHRHHVTHL 309

Query: 304 CGAPVVLNMLTNSPKNKPLDRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETAGLV 363
           CGAPVVLNMLTNSP N+PL+RPVKILTAGAPPPAAVLFRTESLGFDV HGYGLTET GLV
Sbjct: 310 CGAPVVLNMLTNSPDNQPLERPVKILTAGAPPPAAVLFRTESLGFDVGHGYGLTETGGLV 369

Query: 364 VVCSWKPQWKRLPAAERARLKARQGVRTMAITEADVVDPETGKSVKRDGSSIGEIVLKGG 423
           + CSWK +W RLPA ERARLKARQGVRT+A++E DV+D ETGKSVKRDG+SIGEIVL+GG
Sbjct: 370 IYCSWKSKWNRLPATERARLKARQGVRTLAMSEVDVLDSETGKSVKRDGASIGEIVLRGG 429

Query: 424 SIMLGYLKDPEATSKSMIEEGWFFTGDVGVMHGDGYLEIKDRSKDVIISGGENLSSVEVE 483
           S+MLGY KDPEAT K+M +EGWFFTGDVGVMH DGYLEIKDRSKDVIISGGENLSSVEVE
Sbjct: 430 SMMLGYFKDPEATKKAMTDEGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVE 489

Query: 484 SVLYTNPAVNEAAVVARPDEFWGETPCAFVSLREGLSRRPTEEEIIEYCRGKLPRFMVPK 543
           SVLYTNPAVNEAAVVARPDEFWGETPCAFVSLREGL+R PTEEEI+EYCR KLP+FMVPK
Sbjct: 490 SVLYTNPAVNEAAVVARPDEFWGETPCAFVSLREGLTRLPTEEEIMEYCREKLPKFMVPK 549

Query: 544 TVVFKAELPKTSTGKIKKFVLREIAKSMGPARASRL 576
           TVVF  +LPKTSTGKI+KF+LRE AKSMGPAR SRL
Sbjct: 550 TVVFMEKLPKTSTGKIQKFLLREKAKSMGPARTSRL 585

[Close]

BLAST of MS015407 vs. NCBI nr
Match: XP_008463175.1 (PREDICTED: probable acyl-activating enzyme 5, peroxisomal [Cucumis melo] >KAA0046215.1 putative acyl-activating enzyme 5, peroxisomal [Cucumis melo var. makuwa] >TYK18981.1 putative acyl-activating enzyme 5, peroxisomal [Cucumis melo var. makuwa])

HSP 1 Score: 936.0 bits (2418), Expect = 1.5e-268
Identity = 453/555 (81.62%), Postives = 507/555 (91.35%), Query Frame = 0


Query: 22  MEDLKPSPANSSPLTPIGFLDRAATVYGDCPSVIYGSTAYTWSQTHRRCLQLASSLSSNG 81
           M+ LKP+PANSSPLTPI FL+RA+ VY D PS+IY ST++TWSQT+ RCL+LASSLSS G
Sbjct: 1   MDHLKPTPANSSPLTPISFLERASLVYADSPSIIYNSTSFTWSQTYLRCLRLASSLSSLG 60

Query: 82  IHRGHVVSVVAPNTPAMYELQFAVPMAGAVLNNINLRLDARTISILLRHSESKLVFVDQA 141
           IH+GHVVSV++PNTP MYELQF+VPMAGA+L+ INLRLDARTIS+ LRHSESKL+FVDQA
Sbjct: 61  IHKGHVVSVISPNTPPMYELQFSVPMAGAILSCINLRLDARTISVQLRHSESKLLFVDQA 120

Query: 142 SCSLISDALALFPPEARRPALVLIADDAAAEESSSS-PVSGDFVDTYEGMVEKGNPEFRW 201
           S +LISDAL LFPPE++RP LVLIADDAA E+S+SS  + GDFVD YE M++KG+PEFRW
Sbjct: 121 SSALISDALTLFPPESKRPLLVLIADDAAVEDSASSVAIGGDFVDFYEDMIQKGDPEFRW 180

Query: 202 IRPASDWDPIVLNYTSGTTSSPKGVVHCHRGVFMVALDSLLEWGVPKQSVYLWTLPIFHA 261
           IRP S+WDPIV+NYTSGTTSSPKGVVH HRG+F V LDSL+EWGVPK SVYLWTLP+FHA
Sbjct: 181 IRPVSEWDPIVMNYTSGTTSSPKGVVHSHRGIFTVTLDSLIEWGVPKHSVYLWTLPMFHA 240

Query: 262 NGWCFPWGIAAIGGTNVCLRKFDAPLIFRLIHRHNVTHMCGAPVVLNMLTNSPKNKPLDR 321
           NGW FP+ IAA+GGTN+C+RKFDA LIF LI+RH+VTH+CGAPVVLNMLTNSP N+PL+R
Sbjct: 241 NGWSFPFAIAAVGGTNICIRKFDAALIFSLINRHHVTHLCGAPVVLNMLTNSPNNRPLER 300

Query: 322 PVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETAGLVVVCSWKPQWKRLPAAERARLK 381
           PVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTET GLV+ CSWK +W +LPA ERARLK
Sbjct: 301 PVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETGGLVIFCSWKLKWNQLPATERARLK 360

Query: 382 ARQGVRTMAITEADVVDPETGKSVKRDGSSIGEIVLKGGSIMLGYLKDPEATSKSMIEEG 441
           ARQGVRT+A+ EADV+D ETGKSVKRDG SIGEIVL+GGS+MLGY KDPEAT K+M EEG
Sbjct: 361 ARQGVRTLAMAEADVLDSETGKSVKRDGVSIGEIVLRGGSMMLGYFKDPEATKKAMTEEG 420

Query: 442 WFFTGDVGVMHGDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPDEF 501
           WFFTGDVGVMH DGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPDEF
Sbjct: 421 WFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPDEF 480

Query: 502 WGETPCAFVSLREGLSRRPTEEEIIEYCRGKLPRFMVPKTVVFKAELPKTSTGKIKKFVL 561
           WGETPCAFVSLREGLSR PTEEEIIEYCRGKL  FMVPKTVVF  +LPKTSTGKI+KF+L
Sbjct: 481 WGETPCAFVSLREGLSRVPTEEEIIEYCRGKLAGFMVPKTVVFMEQLPKTSTGKIQKFLL 540

Query: 562 REIAKSMGPARASRL 576
           RE+AKSMGP+R SRL
Sbjct: 541 REMAKSMGPSRKSRL 555

[Close]

BLAST of MS015407 vs. ExPASy Swiss-Prot
Match: M4IQQ5 (2-methylpropanoate--CoA ligase CCL4 OS=Humulus lupulus OX=3486 GN=CCL4 PE=1 SV=1)

HSP 1 Score: 847.8 bits (2189), Expect = 7.1e-245
Identity = 394/551 (71.51%), Postives = 482/551 (87.48%), Query Frame = 0


Query: 22  MEDLKPSPANSSPLTPIGFLDRAATVYGDCPSVIYGSTAYTWSQTHRRCLQLASSLSSNG 81
           MEDLKP PA+SSPLTP+GFL+RAATVYGDC SV+Y + +YTWSQTHRRCL LASS++S G
Sbjct: 1   MEDLKPRPASSSPLTPLGFLERAATVYGDCTSVVYDAVSYTWSQTHRRCLCLASSIASLG 60

Query: 82  IHRGHVVSVVAPNTPAMYELQFAVPMAGAVLNNINLRLDARTISILLRHSESKLVFVDQA 141
           I  GHVVSV+APN P MYEL FAVPMAGA+LN +NLRLDARTISILL HSESKL+FVD  
Sbjct: 61  IENGHVVSVLAPNVPQMYELHFAVPMAGAILNAVNLRLDARTISILLHHSESKLIFVDHL 120

Query: 142 SCSLISDALALFPPEARRPALVLIADDAAAEESSSSPVSGDFVDTYEGMVEKGNPEFRWI 201
           S  LI +A+ALFP +A  P LV +AD+  +E  +SS +  +F  +Y+ ++++G+P+F+W+
Sbjct: 121 SRDLILEAIALFPKQAPVPRLVFMADE--SESGNSSELGKEFFCSYKDLIDRGDPDFKWV 180

Query: 202 RPASDWDPIVLNYTSGTTSSPKGVVHCHRGVFMVALDSLLEWGVPKQSVYLWTLPIFHAN 261
            P S+WDP++LNYTSGTTSSPKGVVHCHRG+F++ +DSL++WGVPKQ VYLWTLP+FHAN
Sbjct: 181 MPKSEWDPMILNYTSGTTSSPKGVVHCHRGIFIMTVDSLIDWGVPKQPVYLWTLPMFHAN 240

Query: 262 GWCFPWGIAAIGGTNVCLRKFDAPLIFRLIHRHNVTHMCGAPVVLNMLTNSPKNKPLDRP 321
           GW +PWG+AA+GGTN+CLRKFD+ +I+ +I RH VTHMCGAPVVLNML+N+P ++PL   
Sbjct: 241 GWSYPWGMAAVGGTNICLRKFDSEIIYDMIKRHGVTHMCGAPVVLNMLSNAPGSEPLKTT 300

Query: 322 VKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETAGLVVVCSWKPQWKRLPAAERARLKA 381
           V+I+TAGAPPP+AVLFRTESLGF VSHGYGLTETAGLVV C+WK +W  LPA ERARLK+
Sbjct: 301 VQIMTAGAPPPSAVLFRTESLGFAVSHGYGLTETAGLVVSCAWKKEWNHLPATERARLKS 360

Query: 382 RQGVRTMAITEADVVDPETGKSVKRDGSSIGEIVLKGGSIMLGYLKDPEATSKSMIEEGW 441
           RQGV T+  T+ DVVDP TG +VKRDGS++GE+VL+GGS+MLGYLKDPE T+KSM  +GW
Sbjct: 361 RQGVGTVMQTKIDVVDPVTGAAVKRDGSTLGEVVLRGGSVMLGYLKDPEGTAKSMTADGW 420

Query: 442 FFTGDVGVMHGDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPDEFW 501
           F+TGDVGVMH DGYLEIKDRSKDVIISGGENLSSVEVES+LY++P + EAAVVARPDEFW
Sbjct: 421 FYTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYSHPDILEAAVVARPDEFW 480

Query: 502 GETPCAFVSLREGLSRRPTEEEIIEYCRGKLPRFMVPKTVVFKAELPKTSTGKIKKFVLR 561
           GETPCAFVSL++GL+++PTE+EI+EYCR KLPR+MVPKTVVFK ELPKTSTGK++KF+LR
Sbjct: 481 GETPCAFVSLKKGLTKKPTEKEIVEYCRSKLPRYMVPKTVVFKEELPKTSTGKVQKFILR 540

Query: 562 EIAKSMGPARA 573
           ++A+ MG A A
Sbjct: 541 DMARGMGSATA 549

[Close]

BLAST of MS015407 vs. ExPASy Swiss-Prot
Match: Q9FFE6 (Probable acyl-activating enzyme 5, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=AAE5 PE=1 SV=1)

HSP 1 Score: 805.1 bits (2078), Expect = 5.3e-232
Identity = 389/557 (69.84%), Postives = 468/557 (84.02%), Query Frame = 0


Query: 22  MEDLKPSPANSSPLTPIGFLDRAATVYGDCPSVIYGS-TAYTWSQTHRRCLQLASSLSSN 81
           ME +KP  ANS PLTPIGFL+RAATVYGDC S++YGS T YTW +T+ RCL++ASSLSS 
Sbjct: 1   MEQMKPCAANSPPLTPIGFLERAATVYGDCTSIVYGSNTVYTWRETNLRCLRVASSLSSI 60

Query: 82  GIHRGHVVSVVAPNTPAMYELQFAVPMAGAVLNNINLRLDARTISILLRHSESKLVFVDQ 141
           GI R  VVSV++PNTPAMYELQFAVPM+GA+LNNIN RLDART+S+LLRH  SKL+FVD 
Sbjct: 61  GIGRSDVVSVLSPNTPAMYELQFAVPMSGAILNNINTRLDARTVSVLLRHCGSKLLFVDV 120

Query: 142 ASCSLISDALALFPPEARRPALVLIADDAAAEESSSSPVSG--DFVDTYEGMVEKGNPEF 201
            S  L  +A+++   +   P LV IAD    EE   + V+    F  TY+ ++ +G+ +F
Sbjct: 121 FSVDLAVEAISMMTTDP--PILVFIAD--KEEEGGDADVADRTKFSYTYDDLIHRGDLDF 180

Query: 202 RWIRPASDWDPIVLNYTSGTTSSPKGVVHCHRGVFMVALDSLLEWGVPKQSVYLWTLPIF 261
           +WIRP S+WDP+VLNYTSGTTS+PKGVVHCHRG+F++++DSL++W VPK  VYLWTLPIF
Sbjct: 181 KWIRPESEWDPVVLNYTSGTTSAPKGVVHCHRGIFVMSIDSLIDWTVPKNPVYLWTLPIF 240

Query: 262 HANGWCFPWGIAAIGGTNVCLRKFDAPLIFRLIHRHNVTHMCGAPVVLNMLTNSPKNKPL 321
           HANGW +PWGIAA+GGTNVCLRKFDAPLI+RLI  H VTHMCGAPVVLNML+ + + +PL
Sbjct: 241 HANGWSYPWGIAAVGGTNVCLRKFDAPLIYRLIRDHGVTHMCGAPVVLNMLSATNEFQPL 300

Query: 322 DRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETAGLVVVCSWKPQWKRLPAAERAR 381
           +RPV ILTAGAPPPAAVL R ES+GF +SHGYGLTETAGL V C+WKPQW RLPA++RAR
Sbjct: 301 NRPVNILTAGAPPPAAVLLRAESIGFVISHGYGLTETAGLNVSCAWKPQWNRLPASDRAR 360

Query: 382 LKARQGVRTMAITEADVVDPETGKSVKRDGSSIGEIVLKGGSIMLGYLKDPEATSKSMIE 441
           LKARQGVRT+  TE DVVDPE+G+SV+R+G ++GEIV++G SIMLGYLKDP  T K+ ++
Sbjct: 361 LKARQGVRTVGFTEIDVVDPESGRSVERNGETVGEIVMRGSSIMLGYLKDPVGTEKA-LK 420

Query: 442 EGWFFTGDVGVMHGDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPD 501
            GWF+TGDVGV+H DGYLEIKDRSKD+II+GGEN+SSVEVE+VLYTNPAVNE AVVARPD
Sbjct: 421 NGWFYTGDVGVIHSDGYLEIKDRSKDIIITGGENVSSVEVETVLYTNPAVNEVAVVARPD 480

Query: 502 EFWGETPCAFVSLREGLSRRPTEEEIIEYCRGKLPRFMVPKTVVFKAELPKTSTGKIKKF 561
            FWGETPCAFVSL+ GL++RPTE E+IEYCR K+P++MVPKTV F  ELPKTSTGK+ KF
Sbjct: 481 VFWGETPCAFVSLKSGLTQRPTEVEMIEYCRKKMPKYMVPKTVSFVDELPKTSTGKVMKF 540

Query: 562 VLREIAKSMGPARASRL 576
           VLREIAK MG  R SR+
Sbjct: 541 VLREIAKKMGTTRLSRM 552

[Close]

BLAST of MS015407 vs. ExPASy Swiss-Prot
Match: Q9FFE9 (Probable acyl-activating enzyme 6 OS=Arabidopsis thaliana OX=3702 GN=AAE6 PE=2 SV=1)

HSP 1 Score: 793.5 bits (2048), Expect = 1.6e-228
Identity = 378/550 (68.73%), Postives = 464/550 (84.36%), Query Frame = 0


Query: 22  MEDLKPSPANSSPLTPIGFLDRAATVYGDCPSVIYGS-TAYTWSQTHRRCLQLASSLSSN 81
           ME++KP  ANS PLTPIGFL+RAATVYGDC S++YGS T YTW +T+ RCL++ASSLSS 
Sbjct: 1   MEEMKPCAANSPPLTPIGFLERAATVYGDCTSIVYGSNTVYTWRETNLRCLRVASSLSSI 60

Query: 82  GIHRGHVVSVVAPNTPAMYELQFAVPMAGAVLNNINLRLDARTISILLRHSESKLVFVDQ 141
           GI R  VVSV++PNTPAMYELQFAVPM+GA+LNNIN RLDART+S+LLRH ESKL+FVD 
Sbjct: 61  GIGRSDVVSVLSPNTPAMYELQFAVPMSGAILNNINTRLDARTVSVLLRHCESKLLFVDV 120

Query: 142 ASCSLISDALALFPPEARRPALVLIADDAAAEESSSSPVS--GDFVDTYEGMVEKGNPEF 201
            S  L  +A+++   +   P LV+IAD    EE   + V+    F  TY+ ++E+G+P F
Sbjct: 121 FSVDLAVEAVSMMTTDP--PILVVIAD--KEEEGGVADVADLSKFSYTYDDLIERGDPGF 180

Query: 202 RWIRPASDWDPIVLNYTSGTTSSPKGVVHCHRGVFMVALDSLLEWGVPKQSVYLWTLPIF 261
           +WIRP S+WDP+VLNYTSGTTS+PKGVVHCHRG+F++++DSL++W VPK  VYLWTLPIF
Sbjct: 181 KWIRPESEWDPVVLNYTSGTTSAPKGVVHCHRGIFVMSVDSLIDWAVPKNPVYLWTLPIF 240

Query: 262 HANGWCFPWGIAAIGGTNVCLRKFDAPLIFRLIHRHNVTHMCGAPVVLNMLTNSPKNKPL 321
           H+NGW  PWGIAA+GGTNVCLRKFDAPLI+RLI  H VTHMCGAPVVLNML+ + +++PL
Sbjct: 241 HSNGWTNPWGIAAVGGTNVCLRKFDAPLIYRLIRDHGVTHMCGAPVVLNMLSATQESQPL 300

Query: 322 DRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETAGLVVVCSWKPQWKRLPAAERAR 381
           + PV ILTAG+PPPA VL R ES+GF +SHGYGLTETAG++V C+WKP+W  LPA++RAR
Sbjct: 301 NHPVNILTAGSPPPATVLLRAESIGFVISHGYGLTETAGVIVSCAWKPKWNHLPASDRAR 360

Query: 382 LKARQGVRTMAITEADVVDPETGKSVKRDGSSIGEIVLKGGSIMLGYLKDPEATSKSMIE 441
           LKARQGVRT+  TE DVVDPE+G SV+R+G ++GEIV++G S+MLGYLKDP  T K+ ++
Sbjct: 361 LKARQGVRTVGFTEIDVVDPESGLSVERNGETVGEIVMRGSSVMLGYLKDPVGTEKA-LK 420

Query: 442 EGWFFTGDVGVMHGDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPD 501
            GWF+TGDVGV+H DGYLEIKDRSKD+II+GGEN+SSVEVE+VLYT PAVNE AVVARPD
Sbjct: 421 NGWFYTGDVGVIHSDGYLEIKDRSKDIIITGGENVSSVEVETVLYTIPAVNEVAVVARPD 480

Query: 502 EFWGETPCAFVSLREGLSRRPTEEEIIEYCRGKLPRFMVPKTVVFKAELPKTSTGKIKKF 561
           EFWGETPCAFVSL+ G S +PTEEE++EYCR K+P++MVPKTV F  ELPK+STGK+ KF
Sbjct: 481 EFWGETPCAFVSLKNGFSGKPTEEELMEYCRKKMPKYMVPKTVSFMDELPKSSTGKVTKF 540

Query: 562 VLREIAKSMG 569
           VLR+IAK MG
Sbjct: 541 VLRDIAKKMG 545

[Close]

BLAST of MS015407 vs. ExPASy Swiss-Prot
Match: M4ISH2 (Probable CoA ligase CCL11 OS=Humulus lupulus OX=3486 GN=CCL11 PE=2 SV=1)

HSP 1 Score: 778.5 bits (2009), Expect = 5.3e-224
Identity = 380/554 (68.59%), Postives = 453/554 (81.77%), Query Frame = 0


Query: 22  MEDLKPSPANSSPLTPIGFLDRAATVYGDCPSVIYGSTAYTWSQTHRRCLQLASSLSS-N 81
           ME+LKP  ANS PLTP+GFL+RAATVYGDC SV+Y   +YTWSQTH RCL++AS + S  
Sbjct: 1   MEELKPRFANSCPLTPLGFLERAATVYGDCISVVYDDLSYTWSQTHTRCLRVASCIESCL 60

Query: 82  GIHRGHVVSVVAPNTPAMYELQFAVPMAGAVLNNINLRLDARTISILLRHSESKLVFVDQ 141
           G+ +G VVSVVAPN PAMYEL FAVPMAG VLNNIN RL+A TIS++LRHSESKLVFVDQ
Sbjct: 61  GVKKGQVVSVVAPNVPAMYELNFAVPMAGVVLNNINTRLNAVTISVMLRHSESKLVFVDQ 120

Query: 142 ASCSLISDALALFPPEARRPALVLIADDAAAEESSSSPVSGDFVDTYEGMVEKGNPE-FR 201
            S  L+ DA++LFP     P LVLIAD+   E  +       FV  YE +VEKG+ + F+
Sbjct: 121 LSVRLVLDAVSLFPKNTPTPLLVLIADNITGENLTVED-REHFVCCYEDLVEKGDDKTFK 180

Query: 202 WIRPASDWDPIVLNYTSGTTSSPKGVVHCHRGVFMVALDSLLEWGVPKQSVYLWTLPIFH 261
           W+RP S+WDPIVLNYTSGTTSSPKGVVH HR VF+V LDSL++WGVPKQ VYLWTLP+FH
Sbjct: 181 WVRPISEWDPIVLNYTSGTTSSPKGVVHSHRSVFVVTLDSLIDWGVPKQPVYLWTLPMFH 240

Query: 262 ANGWCFPWGIAAIGGTNVCLRKFDAPLIFRLIHRHNVTHMCGAPVVLNMLTNSPKNKPLD 321
           ANGW + WGIAA+GGTN+CLR+FD  +IF LI RH VTHMC AP+VLNML+NSP  +PL 
Sbjct: 241 ANGWGYTWGIAAVGGTNICLRRFDGEIIFNLIRRHRVTHMCAAPIVLNMLSNSPNAEPLP 300

Query: 322 RPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETAGLVVVCSWKPQWKRLPAAERARL 381
            PV ++T GAPPPAAVL RTESLGF +SHGYG+TE  G+VV C+WK +W RLPA E+ARL
Sbjct: 301 NPVHVMTGGAPPPAAVLLRTESLGFVISHGYGMTEMLGVVVSCAWKREWNRLPATEQARL 360

Query: 382 KARQGVRTMAITEADVVDPETGKSVKRDGSSIGEIVLKGGSIMLGYLKDPEATSKSMIEE 441
           K+RQGVRT A+ E DVVDP +G SVKRDG ++GEIVLKG SIMLGYLK+  AT+K +  +
Sbjct: 361 KSRQGVRTAAMMEVDVVDPNSGVSVKRDGLTMGEIVLKGSSIMLGYLKNSAATAKCIRAD 420

Query: 442 GWFFTGDVGVMHGDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPDE 501
           GWF+TGD+ VMH DGYLEIKDRSKDVIISGGEN+SSVEVES LY++PAV+EAAVVA PDE
Sbjct: 421 GWFYTGDMAVMHPDGYLEIKDRSKDVIISGGENVSSVEVESALYSHPAVDEAAVVACPDE 480

Query: 502 FWGETPCAFVSLREGLSRRPTEEEIIEYCRGKLPRFMVPKTVVFKAELPKTSTGKIKKFV 561
           +WGETP AFV+L++G+  RPTE+EI+EYCR KL  FMVPK VVF+ +LPKTSTGKI+KF 
Sbjct: 481 YWGETPFAFVTLKKGMRVRPTEKEILEYCREKLAHFMVPKVVVFRDKLPKTSTGKIQKFT 540

Query: 562 LREIAKSMGPARAS 574
           L+EI K+MG + +S
Sbjct: 541 LKEIVKTMGYSSSS 553

[Close]

BLAST of MS015407 vs. ExPASy Swiss-Prot
Match: Q9LQS1 (Probable acyl-activating enzyme 8 OS=Arabidopsis thaliana OX=3702 GN=AAE8 PE=2 SV=1)

HSP 1 Score: 760.0 bits (1961), Expect = 2.0e-218
Identity = 369/548 (67.34%), Postives = 446/548 (81.39%), Query Frame = 0


Query: 22  MEDLKPSPANSSPLTPIGFLDRAATVYGDCPSVIYG-STAYTWSQTHRRCLQLASSLSSN 81
           MEDLKPS ANS PLT +GFL+RAATVYGDC S++YG ST YTW +T+ RCL +AS+LSS 
Sbjct: 1   MEDLKPSAANSLPLTLLGFLERAATVYGDCTSIVYGNSTVYTWRETNHRCLCVASALSSI 60

Query: 82  GIHRGHVVSVVAPNTPAMYELQFAVPMAGAVLNNINLRLDARTISILLRHSESKLVFVDQ 141
           GI R  VVSV++ NTP MYELQF+VPM+GA+LNNIN RLDART+S+LLRH ESKL+FVD 
Sbjct: 61  GIGRSDVVSVLSANTPEMYELQFSVPMSGAILNNINTRLDARTVSVLLRHCESKLLFVDF 120

Query: 142 ASCSLISDALALFPPEARRPALVLIADDAAAEESSSSPVSGDFVDTYEGMVEKGNPEFRW 201
               L  +A+ +       P LVLIA++   E  +       F   Y  ++ +GNP+F+W
Sbjct: 121 FYSDLAVEAITML---LNPPILVLIANEEEEEGGAEVTERSKFCYLYSDLITRGNPDFKW 180

Query: 202 IRPASDWDPIVLNYTSGTTSSPKGVVHCHRGVFMVALDSLLEWGVPKQSVYLWTLPIFHA 261
           IRP S+WDPIV+NYTSGTTSSPKGVVHCHRG+F++ LDSL +W VPK  VYLWTLPIFHA
Sbjct: 181 IRPGSEWDPIVVNYTSGTTSSPKGVVHCHRGIFVMTLDSLTDWAVPKTPVYLWTLPIFHA 240

Query: 262 NGWCFPWGIAAIGGTNVCLRKFDAPLIFRLIHRHNVTHMCGAPVVLNMLTNSPK-NKPLD 321
           NGW +PWGIAA+GGTNVC+RK  AP I+ LI  H VTHM GAP+VL +L+ S + ++PL 
Sbjct: 241 NGWTYPWGIAAVGGTNVCVRKLHAPSIYHLIRDHGVTHMYGAPIVLQILSASQESDQPLK 300

Query: 322 RPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETAGLVVVCSWKPQWKRLPAAERARL 381
            PV  LTAG+ PPA VL R ESLGF VSHGYGLTETAG++V C+WKP W RLPA+++A+L
Sbjct: 301 SPVNFLTAGSSPPATVLLRAESLGFIVSHGYGLTETAGVIVSCAWKPNWNRLPASDQAQL 360

Query: 382 KARQGVRTMAITEADVVDPETGKSVKRDGSSIGEIVLKGGSIMLGYLKDPEATSKSMIEE 441
           K+RQGVRT+  +E DVVDPE+G+SV+RDG ++GEIVL+G SIMLGYLK+P  T  S  + 
Sbjct: 361 KSRQGVRTVGFSEIDVVDPESGRSVERDGETVGEIVLRGSSIMLGYLKNPIGTQNS-FKN 420

Query: 442 GWFFTGDVGVMHGDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPDE 501
           GWFFTGD+GV+HGDGYLEIKDRSKDVIISGGEN+SSVEVE+VLYTNPAVNEAAVVARPDE
Sbjct: 421 GWFFTGDLGVIHGDGYLEIKDRSKDVIISGGENVSSVEVEAVLYTNPAVNEAAVVARPDE 480

Query: 502 FWGETPCAFVSLREGLSRRPTEEEIIEYCRGKLPRFMVPKTVVFKAELPKTSTGKIKKFV 561
           FWGETPCAFVSL+ GL+R+PT++EIIEYC+ K+PR+M PKTV F  ELPKTSTGKI K +
Sbjct: 481 FWGETPCAFVSLKPGLTRKPTDKEIIEYCKYKMPRYMAPKTVSFLEELPKTSTGKIIKSL 540

Query: 562 LREIAKSM 568
           L+EIAK+M
Sbjct: 541 LKEIAKNM 544

[Close]

BLAST of MS015407 vs. ExPASy TrEMBL
Match: A0A6J1FL76 (probable acyl-activating enzyme 5, peroxisomal OS=Cucurbita moschata OX=3662 GN=LOC111445243 PE=4 SV=1)

HSP 1 Score: 963.0 bits (2488), Expect = 5.6e-277
Identity = 468/557 (84.02%), Postives = 511/557 (91.74%), Query Frame = 0


Query: 22  MEDLKPSPANSSPLTPIGFLDRAATVYGDCPSVIYGSTAYTWSQTHRRCLQLASSLSSNG 81
           MEDLKP+PANSSPLTPIGFL+RAA VYGDCPS+IY ST YTW++THRRCLQLASSL S G
Sbjct: 1   MEDLKPNPANSSPLTPIGFLERAAVVYGDCPSIIYNSTTYTWTETHRRCLQLASSLVSIG 60

Query: 82  IHRGHVVSVVAPNTPAMYELQFAVPMAGAVLNNINLRLDARTISILLRHSESKLVFVDQA 141
           I RG VVSVV+PNT AMYEL FAVPMAGAVLN INLRLDARTIS++LRH E+ L+FVDQA
Sbjct: 61  IQRGQVVSVVSPNTAAMYELHFAVPMAGAVLNTINLRLDARTISVILRHCETNLIFVDQA 120

Query: 142 SCSLISDALALFPPEARRPALVLIADDAAAEESSSSPV---SGDFVDTYEGMVEKGNPEF 201
           SC LISDALALFPPE +RP L+LIAD+AAAE+SSS+       DFVD+YE MV+KG+P F
Sbjct: 121 SCGLISDALALFPPEMKRPPLILIADNAAAEDSSSASSVWNRADFVDSYEEMVQKGDPLF 180

Query: 202 RWIRPASDWDPIVLNYTSGTTSSPKGVVHCHRGVFMVALDSLLEWGVPKQSVYLWTLPIF 261
           +WIRPAS+WDPIVLNYTSGTTSSPKGVVHCHRG+F V LDSL+EWGVPK+SVYLWTLP+F
Sbjct: 181 QWIRPASEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVTLDSLIEWGVPKESVYLWTLPMF 240

Query: 262 HANGWCFPWGIAAIGGTNVCLRKFDAPLIFRLIHRHNVTHMCGAPVVLNMLTNSPKNKPL 321
           HANGW FPWGIAA+GGTNVC+RKFDA  IF LIHR++VTH+CGAPVVLNMLTNSP N+PL
Sbjct: 241 HANGWSFPWGIAAVGGTNVCIRKFDAAAIFALIHRYHVTHLCGAPVVLNMLTNSPDNRPL 300

Query: 322 DRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETAGLVVVCSWKPQWKRLPAAERAR 381
           DRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTET GLV+ CSWK  W RLPAAERAR
Sbjct: 301 DRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETGGLVICCSWKSGWNRLPAAERAR 360

Query: 382 LKARQGVRTMAITEADVVDPETGKSVKRDGSSIGEIVLKGGSIMLGYLKDPEATSKSMIE 441
           LKARQGVRT+A+  ADV+DPETGKSVKRDG+SIGEIVL+G S+MLGYLKDPEAT+K+M  
Sbjct: 361 LKARQGVRTLAMAVADVLDPETGKSVKRDGTSIGEIVLRGSSMMLGYLKDPEATAKAMTA 420

Query: 442 EGWFFTGDVGVMHGDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPD 501
           EGWFFTGDVGVMH DGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPD
Sbjct: 421 EGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPD 480

Query: 502 EFWGETPCAFVSLREGLSRRPTEEEIIEYCRGKLPRFMVPKTVVFKAELPKTSTGKIKKF 561
           EFWGETPCAFVSL+EGL+RRPTE EIIEYCRGKLPRFMVPKTVVF AELPKTSTGKI+KF
Sbjct: 481 EFWGETPCAFVSLKEGLTRRPTEGEIIEYCRGKLPRFMVPKTVVFTAELPKTSTGKIQKF 540

Query: 562 VLREIAKSMGPARASRL 576
           VLRE+AKSMGPA  SRL
Sbjct: 541 VLREMAKSMGPAGKSRL 557

[Close]

BLAST of MS015407 vs. ExPASy TrEMBL
Match: A0A6J1JTQ2 (probable acyl-activating enzyme 5, peroxisomal OS=Cucurbita maxima OX=3661 GN=LOC111489657 PE=4 SV=1)

HSP 1 Score: 959.5 bits (2479), Expect = 6.2e-276
Identity = 468/561 (83.42%), Postives = 510/561 (90.91%), Query Frame = 0


Query: 22  MEDLKPSPANSSPLTPIGFLDRAATVYGDCPSVIYGSTAYTWSQTHRRCLQLASSLSSNG 81
           MEDLKP+PANSSPLTPIGFL+RAA VYGDCPS+IY ST YTW++THRRCLQLASSL S G
Sbjct: 1   MEDLKPNPANSSPLTPIGFLERAAVVYGDCPSIIYNSTTYTWTETHRRCLQLASSLVSIG 60

Query: 82  IHRGHVVSVVAPNTPAMYELQFAVPMAGAVLNNINLRLDARTISILLRHSESKLVFVDQA 141
           I RG VVSVV+PNTPAMYEL FAVPMAGAVLN INLRLD+RTIS++LRH E+ L+FVDQA
Sbjct: 61  IQRGQVVSVVSPNTPAMYELHFAVPMAGAVLNTINLRLDSRTISVILRHCETNLIFVDQA 120

Query: 142 SCSLISDALALFPPEARRPALVLIADDAAAEESSSSPVS-------GDFVDTYEGMVEKG 201
           SC LISDALALFPPE +RP L+LIAD+AAAE+SSSS  S        DFVD+YE MV+KG
Sbjct: 121 SCGLISDALALFPPEMKRPPLILIADNAAAEDSSSSSSSASSVSNRADFVDSYEEMVQKG 180

Query: 202 NPEFRWIRPASDWDPIVLNYTSGTTSSPKGVVHCHRGVFMVALDSLLEWGVPKQSVYLWT 261
           +P F+WIRP S+WDPIVLNYTSGTTSSPKGVVHCHRG+F V LDSL+EWGV KQSVYLWT
Sbjct: 181 DPLFQWIRPVSEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVTLDSLIEWGVSKQSVYLWT 240

Query: 262 LPIFHANGWCFPWGIAAIGGTNVCLRKFDAPLIFRLIHRHNVTHMCGAPVVLNMLTNSPK 321
           LP+FHANGW FPWGIAA+GGTNVC+RKFDA  IF LIHR++VTH+CGAPVVLNMLTNSP 
Sbjct: 241 LPMFHANGWSFPWGIAAVGGTNVCIRKFDAATIFALIHRYHVTHLCGAPVVLNMLTNSPD 300

Query: 322 NKPLDRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETAGLVVVCSWKPQWKRLPAA 381
           N+PLDRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTET GLV+ CSWK  W RLPAA
Sbjct: 301 NRPLDRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETGGLVICCSWKSGWNRLPAA 360

Query: 382 ERARLKARQGVRTMAITEADVVDPETGKSVKRDGSSIGEIVLKGGSIMLGYLKDPEATSK 441
           ERARLKARQGVRT+A+  ADV+ PETGKSVKRDG+SIGEIVL+G S+MLGYLKDPEAT+K
Sbjct: 361 ERARLKARQGVRTLAMAVADVLCPETGKSVKRDGTSIGEIVLRGSSMMLGYLKDPEATAK 420

Query: 442 SMIEEGWFFTGDVGVMHGDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVV 501
           +M  EGWFFTGDVGVMH DGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVV
Sbjct: 421 AMTAEGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVV 480

Query: 502 ARPDEFWGETPCAFVSLREGLSRRPTEEEIIEYCRGKLPRFMVPKTVVFKAELPKTSTGK 561
           ARPDEFWGETPCAFVSL+EGL+RRPTE EIIEYCRGKLPRFMVPKTVVF AELPKTSTGK
Sbjct: 481 ARPDEFWGETPCAFVSLKEGLTRRPTEGEIIEYCRGKLPRFMVPKTVVFMAELPKTSTGK 540

Query: 562 IKKFVLREIAKSMGPARASRL 576
           I+KFVLRE+AKSMGPA  SRL
Sbjct: 541 IQKFVLREMAKSMGPAGRSRL 561

[Close]

BLAST of MS015407 vs. ExPASy TrEMBL
Match: A0A5A7TXV0 (Putative acyl-activating enzyme 5, peroxisomal OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold418G00770 PE=4 SV=1)

HSP 1 Score: 936.0 bits (2418), Expect = 7.3e-269
Identity = 453/555 (81.62%), Postives = 507/555 (91.35%), Query Frame = 0


Query: 22  MEDLKPSPANSSPLTPIGFLDRAATVYGDCPSVIYGSTAYTWSQTHRRCLQLASSLSSNG 81
           M+ LKP+PANSSPLTPI FL+RA+ VY D PS+IY ST++TWSQT+ RCL+LASSLSS G
Sbjct: 1   MDHLKPTPANSSPLTPISFLERASLVYADSPSIIYNSTSFTWSQTYLRCLRLASSLSSLG 60

Query: 82  IHRGHVVSVVAPNTPAMYELQFAVPMAGAVLNNINLRLDARTISILLRHSESKLVFVDQA 141
           IH+GHVVSV++PNTP MYELQF+VPMAGA+L+ INLRLDARTIS+ LRHSESKL+FVDQA
Sbjct: 61  IHKGHVVSVISPNTPPMYELQFSVPMAGAILSCINLRLDARTISVQLRHSESKLLFVDQA 120

Query: 142 SCSLISDALALFPPEARRPALVLIADDAAAEESSSS-PVSGDFVDTYEGMVEKGNPEFRW 201
           S +LISDAL LFPPE++RP LVLIADDAA E+S+SS  + GDFVD YE M++KG+PEFRW
Sbjct: 121 SSALISDALTLFPPESKRPLLVLIADDAAVEDSASSVAIGGDFVDFYEDMIQKGDPEFRW 180

Query: 202 IRPASDWDPIVLNYTSGTTSSPKGVVHCHRGVFMVALDSLLEWGVPKQSVYLWTLPIFHA 261
           IRP S+WDPIV+NYTSGTTSSPKGVVH HRG+F V LDSL+EWGVPK SVYLWTLP+FHA
Sbjct: 181 IRPVSEWDPIVMNYTSGTTSSPKGVVHSHRGIFTVTLDSLIEWGVPKHSVYLWTLPMFHA 240

Query: 262 NGWCFPWGIAAIGGTNVCLRKFDAPLIFRLIHRHNVTHMCGAPVVLNMLTNSPKNKPLDR 321
           NGW FP+ IAA+GGTN+C+RKFDA LIF LI+RH+VTH+CGAPVVLNMLTNSP N+PL+R
Sbjct: 241 NGWSFPFAIAAVGGTNICIRKFDAALIFSLINRHHVTHLCGAPVVLNMLTNSPNNRPLER 300

Query: 322 PVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETAGLVVVCSWKPQWKRLPAAERARLK 381
           PVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTET GLV+ CSWK +W +LPA ERARLK
Sbjct: 301 PVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETGGLVIFCSWKLKWNQLPATERARLK 360

Query: 382 ARQGVRTMAITEADVVDPETGKSVKRDGSSIGEIVLKGGSIMLGYLKDPEATSKSMIEEG 441
           ARQGVRT+A+ EADV+D ETGKSVKRDG SIGEIVL+GGS+MLGY KDPEAT K+M EEG
Sbjct: 361 ARQGVRTLAMAEADVLDSETGKSVKRDGVSIGEIVLRGGSMMLGYFKDPEATKKAMTEEG 420

Query: 442 WFFTGDVGVMHGDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPDEF 501
           WFFTGDVGVMH DGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPDEF
Sbjct: 421 WFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPDEF 480

Query: 502 WGETPCAFVSLREGLSRRPTEEEIIEYCRGKLPRFMVPKTVVFKAELPKTSTGKIKKFVL 561
           WGETPCAFVSLREGLSR PTEEEIIEYCRGKL  FMVPKTVVF  +LPKTSTGKI+KF+L
Sbjct: 481 WGETPCAFVSLREGLSRVPTEEEIIEYCRGKLAGFMVPKTVVFMEQLPKTSTGKIQKFLL 540

Query: 562 REIAKSMGPARASRL 576
           RE+AKSMGP+R SRL
Sbjct: 541 REMAKSMGPSRKSRL 555

[Close]

BLAST of MS015407 vs. ExPASy TrEMBL
Match: A0A1S3CIM6 (probable acyl-activating enzyme 5, peroxisomal OS=Cucumis melo OX=3656 GN=LOC103501383 PE=4 SV=1)

HSP 1 Score: 936.0 bits (2418), Expect = 7.3e-269
Identity = 453/555 (81.62%), Postives = 507/555 (91.35%), Query Frame = 0


Query: 22  MEDLKPSPANSSPLTPIGFLDRAATVYGDCPSVIYGSTAYTWSQTHRRCLQLASSLSSNG 81
           M+ LKP+PANSSPLTPI FL+RA+ VY D PS+IY ST++TWSQT+ RCL+LASSLSS G
Sbjct: 1   MDHLKPTPANSSPLTPISFLERASLVYADSPSIIYNSTSFTWSQTYLRCLRLASSLSSLG 60

Query: 82  IHRGHVVSVVAPNTPAMYELQFAVPMAGAVLNNINLRLDARTISILLRHSESKLVFVDQA 141
           IH+GHVVSV++PNTP MYELQF+VPMAGA+L+ INLRLDARTIS+ LRHSESKL+FVDQA
Sbjct: 61  IHKGHVVSVISPNTPPMYELQFSVPMAGAILSCINLRLDARTISVQLRHSESKLLFVDQA 120

Query: 142 SCSLISDALALFPPEARRPALVLIADDAAAEESSSS-PVSGDFVDTYEGMVEKGNPEFRW 201
           S +LISDAL LFPPE++RP LVLIADDAA E+S+SS  + GDFVD YE M++KG+PEFRW
Sbjct: 121 SSALISDALTLFPPESKRPLLVLIADDAAVEDSASSVAIGGDFVDFYEDMIQKGDPEFRW 180

Query: 202 IRPASDWDPIVLNYTSGTTSSPKGVVHCHRGVFMVALDSLLEWGVPKQSVYLWTLPIFHA 261
           IRP S+WDPIV+NYTSGTTSSPKGVVH HRG+F V LDSL+EWGVPK SVYLWTLP+FHA
Sbjct: 181 IRPVSEWDPIVMNYTSGTTSSPKGVVHSHRGIFTVTLDSLIEWGVPKHSVYLWTLPMFHA 240

Query: 262 NGWCFPWGIAAIGGTNVCLRKFDAPLIFRLIHRHNVTHMCGAPVVLNMLTNSPKNKPLDR 321
           NGW FP+ IAA+GGTN+C+RKFDA LIF LI+RH+VTH+CGAPVVLNMLTNSP N+PL+R
Sbjct: 241 NGWSFPFAIAAVGGTNICIRKFDAALIFSLINRHHVTHLCGAPVVLNMLTNSPNNRPLER 300

Query: 322 PVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETAGLVVVCSWKPQWKRLPAAERARLK 381
           PVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTET GLV+ CSWK +W +LPA ERARLK
Sbjct: 301 PVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETGGLVIFCSWKLKWNQLPATERARLK 360

Query: 382 ARQGVRTMAITEADVVDPETGKSVKRDGSSIGEIVLKGGSIMLGYLKDPEATSKSMIEEG 441
           ARQGVRT+A+ EADV+D ETGKSVKRDG SIGEIVL+GGS+MLGY KDPEAT K+M EEG
Sbjct: 361 ARQGVRTLAMAEADVLDSETGKSVKRDGVSIGEIVLRGGSMMLGYFKDPEATKKAMTEEG 420

Query: 442 WFFTGDVGVMHGDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPDEF 501
           WFFTGDVGVMH DGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPDEF
Sbjct: 421 WFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPDEF 480

Query: 502 WGETPCAFVSLREGLSRRPTEEEIIEYCRGKLPRFMVPKTVVFKAELPKTSTGKIKKFVL 561
           WGETPCAFVSLREGLSR PTEEEIIEYCRGKL  FMVPKTVVF  +LPKTSTGKI+KF+L
Sbjct: 481 WGETPCAFVSLREGLSRVPTEEEIIEYCRGKLAGFMVPKTVVFMEQLPKTSTGKIQKFLL 540

Query: 562 REIAKSMGPARASRL 576
           RE+AKSMGP+R SRL
Sbjct: 541 REMAKSMGPSRKSRL 555

[Close]

BLAST of MS015407 vs. ExPASy TrEMBL
Match: A0A0A0KT33 (AMP dependent ligase OS=Cucumis sativus OX=3659 GN=Csa_5G317890 PE=4 SV=1)

HSP 1 Score: 929.9 bits (2402), Expect = 5.3e-267
Identity = 453/561 (80.75%), Postives = 506/561 (90.20%), Query Frame = 0


Query: 16  LSVTNSMEDLKPSPANSSPLTPIGFLDRAATVYGDCPSVIYGSTAYTWSQTHRRCLQLAS 75
           LS+  SME LKP+PANSSPLTPIGFL+RA+ +Y D PS+IY ST +TWSQT+RRCL+LAS
Sbjct: 16  LSLPISMEHLKPTPANSSPLTPIGFLERASLIYADSPSIIYSSTTFTWSQTYRRCLRLAS 75

Query: 76  SLSSNGIHRGHVVSVVAPNTPAMYELQFAVPMAGAVLNNINLRLDARTISILLRHSESKL 135
           SLSS GIH+GHVVSV+APNTP MYELQFAVPMAGA+L+ INLRLDARTIS+ LRHSESKL
Sbjct: 76  SLSSIGIHKGHVVSVIAPNTPPMYELQFAVPMAGAILSCINLRLDARTISVQLRHSESKL 135

Query: 136 VFVDQASCSLISDALALFPPEARRPALVLIADDAAAEESSSS-PVSGDFVDTYEGMVEKG 195
           +FVDQAS  LISDALALFPPEA+RP LVLI+DDAA E+S+SS  + GD VD YE MV+KG
Sbjct: 136 LFVDQASSGLISDALALFPPEAKRPLLVLISDDAAVEDSASSVAIGGDVVDLYEDMVQKG 195

Query: 196 NPEFRWIRPASDWDPIVLNYTSGTTSSPKGVVHCHRGVFMVALDSLLEWGVPKQSVYLWT 255
           +PEFRWIRP S+WDPIV+NYTSGTTSSPKGVVH HRG+F V LDSL+EWGVPK SVYLWT
Sbjct: 196 DPEFRWIRPVSEWDPIVMNYTSGTTSSPKGVVHSHRGIFTVTLDSLIEWGVPKHSVYLWT 255

Query: 256 LPIFHANGWCFPWGIAAIGGTNVCLRKFDAPLIFRLIHRHNVTHMCGAPVVLNMLTNSPK 315
           LP+FHANGW FP+ IAA+GGTN+C+RKFDA LIF LI+RH+VTHMCGAPVVLNMLTNSP 
Sbjct: 256 LPMFHANGWSFPFAIAAVGGTNICIRKFDAALIFSLINRHHVTHMCGAPVVLNMLTNSPN 315

Query: 316 NKPLDRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETAGLVVVCSWKPQWKRLPAA 375
           N+PL+RPVKILTAGAPPPAAVL RTESLGFDVSHGYGLTET GLV+ CSWK +W +LPA 
Sbjct: 316 NRPLERPVKILTAGAPPPAAVLLRTESLGFDVSHGYGLTETGGLVIFCSWKLEWNQLPAT 375

Query: 376 ERARLKARQGVRTMAITEADVVDPETGKSVKRDGSSIGEIVLKGGSIMLGYLKDPEATSK 435
           ERARLKARQGVRT+A+ EADV+D  TGK V+RDG SIGEIV++GGS+MLGY KDPEAT K
Sbjct: 376 ERARLKARQGVRTLAMAEADVLDSGTGKRVQRDGVSIGEIVIRGGSMMLGYFKDPEATKK 435

Query: 436 SMIEEGWFFTGDVGVMHGDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVV 495
           +M EEGWFFTGDVGVMH DGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVV
Sbjct: 436 AMTEEGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVV 495

Query: 496 ARPDEFWGETPCAFVSLREGLSRRPTEEEIIEYCRGKLPRFMVPKTVVFKAELPKTSTGK 555
           ARPDEFWGETPCAFVSLREGL+R PTEEEIIEYC+GKL  FMVPKTVVF  +LPKTSTGK
Sbjct: 496 ARPDEFWGETPCAFVSLREGLTRVPTEEEIIEYCKGKLAGFMVPKTVVFMEQLPKTSTGK 555

Query: 556 IKKFVLREIAKSMGPARASRL 576
           I+KF+LRE+AKSMG +  SRL
Sbjct: 556 IQKFLLREMAKSMGCSPKSRL 576

[Close]

BLAST of MS015407 vs. TAIR 10
Match: AT5G16370.1 (acyl activating enzyme 5 )

HSP 1 Score: 805.1 bits (2078), Expect = 3.8e-233
Identity = 389/557 (69.84%), Postives = 468/557 (84.02%), Query Frame = 0


Query: 22  MEDLKPSPANSSPLTPIGFLDRAATVYGDCPSVIYGS-TAYTWSQTHRRCLQLASSLSSN 81
           ME +KP  ANS PLTPIGFL+RAATVYGDC S++YGS T YTW +T+ RCL++ASSLSS 
Sbjct: 1   MEQMKPCAANSPPLTPIGFLERAATVYGDCTSIVYGSNTVYTWRETNLRCLRVASSLSSI 60

Query: 82  GIHRGHVVSVVAPNTPAMYELQFAVPMAGAVLNNINLRLDARTISILLRHSESKLVFVDQ 141
           GI R  VVSV++PNTPAMYELQFAVPM+GA+LNNIN RLDART+S+LLRH  SKL+FVD 
Sbjct: 61  GIGRSDVVSVLSPNTPAMYELQFAVPMSGAILNNINTRLDARTVSVLLRHCGSKLLFVDV 120

Query: 142 ASCSLISDALALFPPEARRPALVLIADDAAAEESSSSPVSG--DFVDTYEGMVEKGNPEF 201
            S  L  +A+++   +   P LV IAD    EE   + V+    F  TY+ ++ +G+ +F
Sbjct: 121 FSVDLAVEAISMMTTDP--PILVFIAD--KEEEGGDADVADRTKFSYTYDDLIHRGDLDF 180

Query: 202 RWIRPASDWDPIVLNYTSGTTSSPKGVVHCHRGVFMVALDSLLEWGVPKQSVYLWTLPIF 261
           +WIRP S+WDP+VLNYTSGTTS+PKGVVHCHRG+F++++DSL++W VPK  VYLWTLPIF
Sbjct: 181 KWIRPESEWDPVVLNYTSGTTSAPKGVVHCHRGIFVMSIDSLIDWTVPKNPVYLWTLPIF 240

Query: 262 HANGWCFPWGIAAIGGTNVCLRKFDAPLIFRLIHRHNVTHMCGAPVVLNMLTNSPKNKPL 321
           HANGW +PWGIAA+GGTNVCLRKFDAPLI+RLI  H VTHMCGAPVVLNML+ + + +PL
Sbjct: 241 HANGWSYPWGIAAVGGTNVCLRKFDAPLIYRLIRDHGVTHMCGAPVVLNMLSATNEFQPL 300

Query: 322 DRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETAGLVVVCSWKPQWKRLPAAERAR 381
           +RPV ILTAGAPPPAAVL R ES+GF +SHGYGLTETAGL V C+WKPQW RLPA++RAR
Sbjct: 301 NRPVNILTAGAPPPAAVLLRAESIGFVISHGYGLTETAGLNVSCAWKPQWNRLPASDRAR 360

Query: 382 LKARQGVRTMAITEADVVDPETGKSVKRDGSSIGEIVLKGGSIMLGYLKDPEATSKSMIE 441
           LKARQGVRT+  TE DVVDPE+G+SV+R+G ++GEIV++G SIMLGYLKDP  T K+ ++
Sbjct: 361 LKARQGVRTVGFTEIDVVDPESGRSVERNGETVGEIVMRGSSIMLGYLKDPVGTEKA-LK 420

Query: 442 EGWFFTGDVGVMHGDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPD 501
            GWF+TGDVGV+H DGYLEIKDRSKD+II+GGEN+SSVEVE+VLYTNPAVNE AVVARPD
Sbjct: 421 NGWFYTGDVGVIHSDGYLEIKDRSKDIIITGGENVSSVEVETVLYTNPAVNEVAVVARPD 480

Query: 502 EFWGETPCAFVSLREGLSRRPTEEEIIEYCRGKLPRFMVPKTVVFKAELPKTSTGKIKKF 561
            FWGETPCAFVSL+ GL++RPTE E+IEYCR K+P++MVPKTV F  ELPKTSTGK+ KF
Sbjct: 481 VFWGETPCAFVSLKSGLTQRPTEVEMIEYCRKKMPKYMVPKTVSFVDELPKTSTGKVMKF 540

Query: 562 VLREIAKSMGPARASRL 576
           VLREIAK MG  R SR+
Sbjct: 541 VLREIAKKMGTTRLSRM 552

[Close]

BLAST of MS015407 vs. TAIR 10
Match: AT5G16340.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 793.5 bits (2048), Expect = 1.1e-229
Identity = 378/550 (68.73%), Postives = 464/550 (84.36%), Query Frame = 0


Query: 22  MEDLKPSPANSSPLTPIGFLDRAATVYGDCPSVIYGS-TAYTWSQTHRRCLQLASSLSSN 81
           ME++KP  ANS PLTPIGFL+RAATVYGDC S++YGS T YTW +T+ RCL++ASSLSS 
Sbjct: 1   MEEMKPCAANSPPLTPIGFLERAATVYGDCTSIVYGSNTVYTWRETNLRCLRVASSLSSI 60

Query: 82  GIHRGHVVSVVAPNTPAMYELQFAVPMAGAVLNNINLRLDARTISILLRHSESKLVFVDQ 141
           GI R  VVSV++PNTPAMYELQFAVPM+GA+LNNIN RLDART+S+LLRH ESKL+FVD 
Sbjct: 61  GIGRSDVVSVLSPNTPAMYELQFAVPMSGAILNNINTRLDARTVSVLLRHCESKLLFVDV 120

Query: 142 ASCSLISDALALFPPEARRPALVLIADDAAAEESSSSPVS--GDFVDTYEGMVEKGNPEF 201
            S  L  +A+++   +   P LV+IAD    EE   + V+    F  TY+ ++E+G+P F
Sbjct: 121 FSVDLAVEAVSMMTTDP--PILVVIAD--KEEEGGVADVADLSKFSYTYDDLIERGDPGF 180

Query: 202 RWIRPASDWDPIVLNYTSGTTSSPKGVVHCHRGVFMVALDSLLEWGVPKQSVYLWTLPIF 261
           +WIRP S+WDP+VLNYTSGTTS+PKGVVHCHRG+F++++DSL++W VPK  VYLWTLPIF
Sbjct: 181 KWIRPESEWDPVVLNYTSGTTSAPKGVVHCHRGIFVMSVDSLIDWAVPKNPVYLWTLPIF 240

Query: 262 HANGWCFPWGIAAIGGTNVCLRKFDAPLIFRLIHRHNVTHMCGAPVVLNMLTNSPKNKPL 321
           H+NGW  PWGIAA+GGTNVCLRKFDAPLI+RLI  H VTHMCGAPVVLNML+ + +++PL
Sbjct: 241 HSNGWTNPWGIAAVGGTNVCLRKFDAPLIYRLIRDHGVTHMCGAPVVLNMLSATQESQPL 300

Query: 322 DRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETAGLVVVCSWKPQWKRLPAAERAR 381
           + PV ILTAG+PPPA VL R ES+GF +SHGYGLTETAG++V C+WKP+W  LPA++RAR
Sbjct: 301 NHPVNILTAGSPPPATVLLRAESIGFVISHGYGLTETAGVIVSCAWKPKWNHLPASDRAR 360

Query: 382 LKARQGVRTMAITEADVVDPETGKSVKRDGSSIGEIVLKGGSIMLGYLKDPEATSKSMIE 441
           LKARQGVRT+  TE DVVDPE+G SV+R+G ++GEIV++G S+MLGYLKDP  T K+ ++
Sbjct: 361 LKARQGVRTVGFTEIDVVDPESGLSVERNGETVGEIVMRGSSVMLGYLKDPVGTEKA-LK 420

Query: 442 EGWFFTGDVGVMHGDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPD 501
            GWF+TGDVGV+H DGYLEIKDRSKD+II+GGEN+SSVEVE+VLYT PAVNE AVVARPD
Sbjct: 421 NGWFYTGDVGVIHSDGYLEIKDRSKDIIITGGENVSSVEVETVLYTIPAVNEVAVVARPD 480

Query: 502 EFWGETPCAFVSLREGLSRRPTEEEIIEYCRGKLPRFMVPKTVVFKAELPKTSTGKIKKF 561
           EFWGETPCAFVSL+ G S +PTEEE++EYCR K+P++MVPKTV F  ELPK+STGK+ KF
Sbjct: 481 EFWGETPCAFVSLKNGFSGKPTEEELMEYCRKKMPKYMVPKTVSFMDELPKSSTGKVTKF 540

Query: 562 VLREIAKSMG 569
           VLR+IAK MG
Sbjct: 541 VLRDIAKKMG 545

[Close]

BLAST of MS015407 vs. TAIR 10
Match: AT1G75960.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 760.0 bits (1961), Expect = 1.4e-219
Identity = 369/548 (67.34%), Postives = 446/548 (81.39%), Query Frame = 0


Query: 22  MEDLKPSPANSSPLTPIGFLDRAATVYGDCPSVIYG-STAYTWSQTHRRCLQLASSLSSN 81
           MEDLKPS ANS PLT +GFL+RAATVYGDC S++YG ST YTW +T+ RCL +AS+LSS 
Sbjct: 1   MEDLKPSAANSLPLTLLGFLERAATVYGDCTSIVYGNSTVYTWRETNHRCLCVASALSSI 60

Query: 82  GIHRGHVVSVVAPNTPAMYELQFAVPMAGAVLNNINLRLDARTISILLRHSESKLVFVDQ 141
           GI R  VVSV++ NTP MYELQF+VPM+GA+LNNIN RLDART+S+LLRH ESKL+FVD 
Sbjct: 61  GIGRSDVVSVLSANTPEMYELQFSVPMSGAILNNINTRLDARTVSVLLRHCESKLLFVDF 120

Query: 142 ASCSLISDALALFPPEARRPALVLIADDAAAEESSSSPVSGDFVDTYEGMVEKGNPEFRW 201
               L  +A+ +       P LVLIA++   E  +       F   Y  ++ +GNP+F+W
Sbjct: 121 FYSDLAVEAITML---LNPPILVLIANEEEEEGGAEVTERSKFCYLYSDLITRGNPDFKW 180

Query: 202 IRPASDWDPIVLNYTSGTTSSPKGVVHCHRGVFMVALDSLLEWGVPKQSVYLWTLPIFHA 261
           IRP S+WDPIV+NYTSGTTSSPKGVVHCHRG+F++ LDSL +W VPK  VYLWTLPIFHA
Sbjct: 181 IRPGSEWDPIVVNYTSGTTSSPKGVVHCHRGIFVMTLDSLTDWAVPKTPVYLWTLPIFHA 240

Query: 262 NGWCFPWGIAAIGGTNVCLRKFDAPLIFRLIHRHNVTHMCGAPVVLNMLTNSPK-NKPLD 321
           NGW +PWGIAA+GGTNVC+RK  AP I+ LI  H VTHM GAP+VL +L+ S + ++PL 
Sbjct: 241 NGWTYPWGIAAVGGTNVCVRKLHAPSIYHLIRDHGVTHMYGAPIVLQILSASQESDQPLK 300

Query: 322 RPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETAGLVVVCSWKPQWKRLPAAERARL 381
            PV  LTAG+ PPA VL R ESLGF VSHGYGLTETAG++V C+WKP W RLPA+++A+L
Sbjct: 301 SPVNFLTAGSSPPATVLLRAESLGFIVSHGYGLTETAGVIVSCAWKPNWNRLPASDQAQL 360

Query: 382 KARQGVRTMAITEADVVDPETGKSVKRDGSSIGEIVLKGGSIMLGYLKDPEATSKSMIEE 441
           K+RQGVRT+  +E DVVDPE+G+SV+RDG ++GEIVL+G SIMLGYLK+P  T  S  + 
Sbjct: 361 KSRQGVRTVGFSEIDVVDPESGRSVERDGETVGEIVLRGSSIMLGYLKNPIGTQNS-FKN 420

Query: 442 GWFFTGDVGVMHGDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPDE 501
           GWFFTGD+GV+HGDGYLEIKDRSKDVIISGGEN+SSVEVE+VLYTNPAVNEAAVVARPDE
Sbjct: 421 GWFFTGDLGVIHGDGYLEIKDRSKDVIISGGENVSSVEVEAVLYTNPAVNEAAVVARPDE 480

Query: 502 FWGETPCAFVSLREGLSRRPTEEEIIEYCRGKLPRFMVPKTVVFKAELPKTSTGKIKKFV 561
           FWGETPCAFVSL+ GL+R+PT++EIIEYC+ K+PR+M PKTV F  ELPKTSTGKI K +
Sbjct: 481 FWGETPCAFVSLKPGLTRKPTDKEIIEYCKYKMPRYMAPKTVSFLEELPKTSTGKIIKSL 540

Query: 562 LREIAKSM 568
           L+EIAK+M
Sbjct: 541 LKEIAKNM 544

[Close]

BLAST of MS015407 vs. TAIR 10
Match: AT1G21540.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 730.3 bits (1884), Expect = 1.2e-210
Identity = 350/552 (63.41%), Postives = 445/552 (80.62%), Query Frame = 0


Query: 22  MEDLKPSPANSSPLTPIGFLDRAATVYGDCPSVIY-GSTAYTWSQTHRRCLQLASSLSSN 81
           ME L P P+NS+PLT +GFLDRAA+VYGDCPS+++  +T +TWS+TH RCL++AS+L+S+
Sbjct: 1   MELLLPHPSNSTPLTVLGFLDRAASVYGDCPSILHTTNTVHTWSETHNRCLRIASALTSS 60

Query: 82  --GIHRGHVVSVVAPNTPAMYELQFAVPMAGAVLNNINLRLDARTISILLRHSESKLVFV 141
             GI+RG VVSVV PN P++YELQFAVPM+GA+LNNIN RLDA  +S+LLRHSESKLVFV
Sbjct: 61  SLGINRGQVVSVVGPNVPSVYELQFAVPMSGAILNNINPRLDAHALSVLLRHSESKLVFV 120

Query: 142 DQASCSLISDALALFPPEARRPALVLIADDAAAEESSSSP-VSGDFVDTYEGMVEKGNPE 201
           D  S S++ +A++ F  +  +P LVL+ DD   E+ S SP  + DF+DTY+G++E+G+  
Sbjct: 121 DPNSISVVLEAVS-FMRQNEKPHLVLLDDD--QEDGSLSPSAASDFLDTYQGVMERGDSR 180

Query: 202 FRWIRPASDWDPIVLNYTSGTTSSPKGVVHCHRGVFMVALDSLLEWGVPKQSVYLWTLPI 261
           F+WIRP ++W P++LNYTSGTTSSPKGVV  HR +FM+ + SLL+W  P + VYLWTLP+
Sbjct: 181 FKWIRPQTEWQPMILNYTSGTTSSPKGVVLSHRAIFMLTVSSLLDWHFPNRPVYLWTLPM 240

Query: 262 FHANGWCFPWGIAAIGGTNVCLRKFDAPLIFRLIHRHNVTHMCGAPVVLNMLTNSPKNKP 321
           FHANGW + WG AA+G TNVC R+ DAP I+ LI +H+VTHMC AP+VLNMLTN P  KP
Sbjct: 241 FHANGWGYTWGTAAVGATNVCTRRVDAPTIYDLIDKHHVTHMCAAPMVLNMLTNYPSRKP 300

Query: 322 LDRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETAGLVVVCSWKPQWKRLPAAERA 381
           L  PV+++TAGAPPPAA++ R E+LGF+V HGYGLTET G VV C+WK +W  L   ERA
Sbjct: 301 LKNPVQVMTAGAPPPAAIISRAETLGFNVGHGYGLTETGGPVVSCAWKAEWDHLDPLERA 360

Query: 382 RLKARQGVRTMAITEADVVDPETGKSVKRDGSSIGEIVLKGGSIMLGYLKDPEATSKSMI 441
           RLK+RQGVRT+   E DV DP TGKSV+ DG S+GEIVLKGGS+MLGY KDPE T+  M 
Sbjct: 361 RLKSRQGVRTIGFAEVDVRDPRTGKSVEHDGVSVGEIVLKGGSVMLGYYKDPEGTAACMR 420

Query: 442 EEGWFFTGDVGVMHGDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARP 501
           E+GWF++GDVGV+H DGYLE+KDRSKDVII GGEN+SS EVE+VLYTNP V EAAVVA+P
Sbjct: 421 EDGWFYSGDVGVIHEDGYLEVKDRSKDVIICGGENISSAEVETVLYTNPVVKEAAVVAKP 480

Query: 502 DEFWGETPCAFVSLREGLSRR--PTEEEIIEYCRGKLPRFMVPKTVVFKAELPKTSTGKI 561
           D+ WGETPCAFVSL+   +     TE EI E+C+ +LP++MVP+ V+F+ ELPKTSTGKI
Sbjct: 481 DKMWGETPCAFVSLKYDSNGNGLVTEREIREFCKTRLPKYMVPRKVIFQEELPKTSTGKI 540

Query: 562 KKFVLREIAKSM 568
           +KF+LR++AKS+
Sbjct: 541 QKFLLRQMAKSL 549

[Close]

BLAST of MS015407 vs. TAIR 10
Match: AT1G21530.2 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 728.0 bits (1878), Expect = 5.8e-210
Identity = 348/553 (62.93%), Postives = 444/553 (80.29%), Query Frame = 0


Query: 22  MEDLKPSPANSSPLTPIGFLDRAATVYGDCPSVIY-GSTAYTWSQTHRRCLQLASSLSSN 81
           ME L P P+NS+PLT +GFLDRAA+VYGDCPS+++  +T +TWS+TH RCL++AS+L+S+
Sbjct: 1   MELLLPHPSNSTPLTVLGFLDRAASVYGDCPSILHTANTVHTWSETHNRCLRIASALTSS 60

Query: 82  --GIHRGHVVSVVAPNTPAMYELQFAVPMAGAVLNNINLRLDARTISILLRHSESKLVFV 141
             GI +G VVSVV PN P++YELQFAVPM+GA+LNNIN RLDA  +S+LLRHSES+LVFV
Sbjct: 61  SIGIKQGQVVSVVGPNVPSVYELQFAVPMSGAILNNINPRLDAHALSVLLRHSESRLVFV 120

Query: 142 DQASCSLISDALALFPPEARRPALVLIADDAAAEESSSSPVSGDFVDTYEGMVEKGNPEF 201
           D  S SL+ +A++LF  +  +P LVL+ DD   + SS+S    DF+DTYE ++E+GN  F
Sbjct: 121 DHRSISLVLEAVSLF-TQHEKPHLVLLDDDQENDSSSAS----DFLDTYEEIMERGNSRF 180

Query: 202 RWIRPASDWDPIVLNYTSGTTSSPKGVVHCHRGVFMVALDSLLEWGVPKQSVYLWTLPIF 261
           +WIRP ++W P+VLNYTSGTTSSPKGVV  HR +FM+ + SLL+W VP + VYLWTLP+F
Sbjct: 181 KWIRPQTEWQPMVLNYTSGTTSSPKGVVLSHRAIFMLTVSSLLDWSVPNRPVYLWTLPMF 240

Query: 262 HANGWCFPWGIAAIGGTNVCLRKFDAPLIFRLIHRHNVTHMCGAPVVLNMLTNSPKNKPL 321
           HANGW + WG AA+G TN+C R+ DAP I+ LI +HNVTHMC AP+VLNML N P + PL
Sbjct: 241 HANGWGYTWGTAAVGATNICTRRVDAPTIYNLIDKHNVTHMCAAPMVLNMLINYPLSTPL 300

Query: 322 DRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETAGLVVVCSWKPQWKRLPAAERAR 381
             PV+++T+GAPPPA ++ R ESLGF+VSH YGLTET+G VV C+WKP+W  L   ERAR
Sbjct: 301 KNPVQVMTSGAPPPATIISRAESLGFNVSHSYGLTETSGPVVSCAWKPKWDHLDPLERAR 360

Query: 382 LKARQGVRTMAITEADVVDPETGKSVKRDGSSIGEIVLKGGSIMLGYLKDPEATSKSMIE 441
           LK+RQGVRT+  TE DV D +TGKSVK DG S+GEIV +G S+MLGY KDP+ T+  M E
Sbjct: 361 LKSRQGVRTLGFTEVDVRDRKTGKSVKHDGVSVGEIVFRGSSVMLGYYKDPQGTAACMRE 420

Query: 442 EGWFFTGDVGVMHGDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPD 501
           +GWF++GD+GV+H DGYLEIKDRSKDVII GGEN+SS E+E+VLYTNP V EAAVVA+PD
Sbjct: 421 DGWFYSGDIGVIHKDGYLEIKDRSKDVIICGGENISSAEIETVLYTNPVVKEAAVVAKPD 480

Query: 502 EFWGETPCAFVSLR---EGLSRRP-TEEEIIEYCRGKLPRFMVPKTVVFKAELPKTSTGK 561
           + WGETPCAFVSL+    G    P TE EI E+C+ KLP++MVP+ V+F+ ELPKTSTGK
Sbjct: 481 KMWGETPCAFVSLKCDNNGDGSVPVTEREIREFCKTKLPKYMVPRKVIFQEELPKTSTGK 540

Query: 562 IKKFVLREIAKSM 568
           I+KF+LR++AK++
Sbjct: 541 IQKFLLRQMAKTL 548

The following BLAST results are available for this feature:

BLAST of MS015407 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022939278.1	1.2e-276	84.02	probable acyl-activating enzyme 5, peroxisomal [Cucurbita moschata] >KAG6579057....	[more]
XP_022993747.1	1.3e-275	83.42	probable acyl-activating enzyme 5, peroxisomal [Cucurbita maxima]	[more]
XP_023550909.1	1.3e-275	83.42	probable acyl-activating enzyme 5, peroxisomal [Cucurbita pepo subsp. pepo]	[more]
XP_038877068.1	6.0e-273	80.56	probable acyl-activating enzyme 5, peroxisomal [Benincasa hispida]	[more]
XP_008463175.1	1.5e-268	81.62	PREDICTED: probable acyl-activating enzyme 5, peroxisomal [Cucumis melo] >KAA004...	[more]

BLAST of MS015407 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
M4IQQ5	7.1e-245	71.51	2-methylpropanoate--CoA ligase CCL4 OS=Humulus lupulus OX=3486 GN=CCL4 PE=1 SV=1	[more]
Q9FFE6	5.3e-232	69.84	Probable acyl-activating enzyme 5, peroxisomal OS=Arabidopsis thaliana OX=3702 G...	[more]
Q9FFE9	1.6e-228	68.73	Probable acyl-activating enzyme 6 OS=Arabidopsis thaliana OX=3702 GN=AAE6 PE=2 S...	[more]
M4ISH2	5.3e-224	68.59	Probable CoA ligase CCL11 OS=Humulus lupulus OX=3486 GN=CCL11 PE=2 SV=1	[more]
Q9LQS1	2.0e-218	67.34	Probable acyl-activating enzyme 8 OS=Arabidopsis thaliana OX=3702 GN=AAE8 PE=2 S...	[more]

BLAST of MS015407 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FL76	5.6e-277	84.02	probable acyl-activating enzyme 5, peroxisomal OS=Cucurbita moschata OX=3662 GN=...	[more]
A0A6J1JTQ2	6.2e-276	83.42	probable acyl-activating enzyme 5, peroxisomal OS=Cucurbita maxima OX=3661 GN=LO...	[more]
A0A5A7TXV0	7.3e-269	81.62	Putative acyl-activating enzyme 5, peroxisomal OS=Cucumis melo var. makuwa OX=11...	[more]
A0A1S3CIM6	7.3e-269	81.62	probable acyl-activating enzyme 5, peroxisomal OS=Cucumis melo OX=3656 GN=LOC103...	[more]
A0A0A0KT33	5.3e-267	80.75	AMP dependent ligase OS=Cucumis sativus OX=3659 GN=Csa_5G317890 PE=4 SV=1	[more]

BLAST of MS015407 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G16370.1	3.8e-233	69.84	acyl activating enzyme 5	[more]
AT5G16340.1	1.1e-229	68.73	AMP-dependent synthetase and ligase family protein	[more]
AT1G75960.1	1.4e-219	67.34	AMP-dependent synthetase and ligase family protein	[more]
AT1G21540.1	1.2e-210	63.41	AMP-dependent synthetase and ligase family protein	[more]
AT1G21530.2	5.8e-210	62.93	AMP-dependent synthetase and ligase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR042099	ANL, N-terminal domain	GENE3D	3.40.50.12780		coord: 21..463 e-value: 5.5E-99 score: 333.5
IPR000873	AMP-dependent synthetase/ligase	PFAM	PF00501	AMP-binding	coord: 42..468 e-value: 9.2E-93 score: 311.1
None	No IPR available	GENE3D	3.30.300.30		coord: 464..573 e-value: 9.9E-30 score: 105.2
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 13..31
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..31
None	No IPR available	PANTHER	PTHR43859:SF50	LONG-CHAIN ACYL-COA SYNTHETASE	coord: 23..570
None	No IPR available	PANTHER	PTHR43859	ACYL-ACTIVATING ENZYME	coord: 23..570
None	No IPR available	CDD	cd12118	ttLC_FACS_AEE21_like	coord: 33..562 e-value: 0.0 score: 828.864
None	No IPR available	SUPERFAMILY	56801	Acetyl-CoA synthetase-like	coord: 30..566
IPR025110	AMP-binding enzyme, C-terminal domain	PFAM	PF13193	AMP-binding_C	coord: 477..554 e-value: 1.1E-23 score: 84.0
IPR020845	AMP-binding, conserved site	PROSITE	PS00455	AMP_BINDING	coord: 212..223

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS015407.1	MS015407.1	mRNA