MS015396 (gene) Bitter gourd (TR) v1

Overview


Name	MS015396
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	WRKY domain-containing protein
Location	scaffold586: 28734 .. 31243 (-)
RNA-Seq Expression	MS015396
Synteny	MS015396

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATGGAACAATATTTCCCATCAACTTCCCTCTCGCTGCACCAAATGCTGATTTCTCGAGCTCCCGAGCTCGCCGGGATCCATGGAGCTGGCCCGTTACAGCGCCCGCACGCCTCCGGTGGGTTTCCGGTGTTTCGCGATGTTTCTATCGACGACATGTCGGTCGATGGAGTGAGGAGTATGAAGGATGACGTCGTTTCGCTGAGTAGAAGCGTCCGTTTTGTTGATGGGTCAGCCGGGGAGGGAGTGGATGAGTTGGAGAAGAAAGCGGCGGGGGCCGGGACGGGTACAAGGACGACGGGGCCGGAGGCAGGGCGGGGAGATTTTGAGAAGAAGAAGAGGAGGCAGAGATATGCCTTTCAAACCAGGAGTCAAGTTGATATTCTTGATGATGGTTATAGATGGAGAAAATATGGACAAAAGGCTGTGAAGAACAACAAATTCCCAAGGTAATTCAATTCTACTTTCTTGTTGGTTTTTCTTTTTCTTTTTTTTAACTTTTATATATAATAGTGCATGTGTTGCGTTGAGATTCAAACCTTATTCGATTCATATTGAAGGTTGTTTGTTCGTGGATTTTTTTTTTCGGCAAAATTTGCATAAAAAAATTAGAGTAATAATTTGAATCTACTCTATTTTAAGAGAAGTTTAGAGAGTAATTTAGGAATTAATAGTAATTTAGTTGGCATTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATATCGGTGGTAATTGAATTTTTATTATTCTCAAATGTTGATAATCCACCTTAGATATAGTTACTTTATAATAAAGTTTGGCAACATGAATATAGTTAACTTAAGACACGTATATTCGACCAAAAAATCTCACATGTTGTTGTACTAAAAAGATATATATGTTTGAGTAACAAATCGTTTTTATTTTTATTTTTATTATTATTATTATTTTGCATTCTTTAACATCAATTTTATTCTTAATATATTGAAAATTTTCAAGGACAAATATACTAAAATTTATGTTAATAGAGGAGAGTTGATGTGTCATTAAATTTTGTTCATATATTTGGATATGTAGGTATGAGAAATCCATTTCAAGCGAGTCAAATTTTATTGATTATGCCAATTGTCATTTTTGTATGTCAAATAGCATTGGAGTTATATTCATGTGTTCAAATAAAATTAAACAATAAGTTAATAATACATCAACTTTTTTAAATAAATTTTAATAGTTCTTTTTTAGGGAATAAACTAATTGAAAACTTGAAAACTTTTTATATATCAAGGGCAAGGGTAAAATTGAGAATCCCAAAAATCAATAAACCAACCATTGACATTTGGTAGCGTTACCACTCATAAATCTTTTTAGGTTGAAATCTTTGTTAATATTGGATAACAATTTTTGTACCTTTTAACATTGGATAGATATGATAATTATGTTCTTAGCGTTAAAAGATGCACTTAAAAAGTCAATGGTATTAATTATTTTAAAGTTAAATATAATTTTTCTTCTCTATAATTTAAGTGCTTCAATTTGTTGATATGATTTTATAAACCAACAATGCCTACTCATGCTGTTATGATAAGGTAACCATATTATCTTAAAATCGCTTTCAAATTCTAAGCCAAATTAAAAAAAAAAAAAAAAAGAATTTCAAAATATTATTTTTGTTTTTTTAAGTTTGATTAAGAATTCATATTTGTTTTAAAGAAAATGAAAAACATACTAAATAAATAATGAAAACATAAGTATAACTTTTAAAAACAAAAAAAAAACAAAAAACGAAATCATTGTCAAACCAAATCAACAATCCTAAAGGTAGAAAAATAAAATAAAACAAAACTCATTTGATAACAATTAATCAATATTAGATTGATTTTTTTTAGTACAACTAGTGGGGCGGGAGATTCGAATTCATGGCTTTGACCGGTGACATTTACCGTTGAGCTAATTTCATATTGACTCAATACCTAGATTAATTAGCCATATATGTCATATATCTTTTTATAGTTGATTACTAGTAACAACCAATTAATTAGCACTCCTTCCGTAAGGTCAAAGATTCAAATCCACGTATCCTAAATATATAAAGATGACTAGAATATAATGTATGAGTGAAGGAACCTTCAATAGCGGGAAATGTATATGTGTCTTAACAACTAAACTATATACTCGACTTCAGAGTAATTAATTAAACCCTATATTCAATACTTAACTAATAATAAACTAGCTAGCGTAGATAATGAAGTTAATAATGATTAGGGTTGATTGAATTATTTGGGTGAAATAATTATGTTGATAACATTTTGTTTGGGTGATGAATTGAAAAATTGCAGAAGCTACTATAGATGTACACACCAAGGTTGCAAAGTGAAGAAGCAAGTGCAAAGACTGACTAAAGATGAAGCTGTGGTTGTGACCACTTATGAAGGCATTCACTCTCATCCCATTGACAAATCCACTGATAACTTTGAGCATATTTTGACTCAGATGCAAATTTACACTTCTCAT

mRNA sequence

ATGATGGAACAATATTTCCCATCAACTTCCCTCTCGCTGCACCAAATGCTGATTTCTCGAGCTCCCGAGCTCGCCGGGATCCATGGAGCTGGCCCGTTACAGCGCCCGCACGCCTCCGGTGGGTTTCCGGTGTTTCGCGATGTTTCTATCGACGACATGTCGGTCGATGGAGTGAGGAGTATGAAGGATGACGTCGTTTCGCTGAGTAGAAGCGTCCGTTTTGTTGATGGGTCAGCCGGGGAGGGAGTGGATGAGTTGGAGAAGAAAGCGGCGGGGGCCGGGACGGGTACAAGGACGACGGGGCCGGAGGCAGGGCGGGGAGATTTTGAGAAGAAGAAGAGGAGGCAGAGATATGCCTTTCAAACCAGGAGTCAAGTTGATATTCTTGATGATGGTTATAGATGGAGAAAATATGGACAAAAGGCTGTGAAGAACAACAAATTCCCAAGAAGCTACTATAGATGTACACACCAAGGTTGCAAAGTGAAGAAGCAAGTGCAAAGACTGACTAAAGATGAAGCTGTGGTTGTGACCACTTATGAAGGCATTCACTCTCATCCCATTGACAAATCCACTGATAACTTTGAGCATATTTTGACTCAGATGCAAATTTACACTTCTCAT

Coding sequence (CDS)

ATGATGGAACAATATTTCCCATCAACTTCCCTCTCGCTGCACCAAATGCTGATTTCTCGAGCTCCCGAGCTCGCCGGGATCCATGGAGCTGGCCCGTTACAGCGCCCGCACGCCTCCGGTGGGTTTCCGGTGTTTCGCGATGTTTCTATCGACGACATGTCGGTCGATGGAGTGAGGAGTATGAAGGATGACGTCGTTTCGCTGAGTAGAAGCGTCCGTTTTGTTGATGGGTCAGCCGGGGAGGGAGTGGATGAGTTGGAGAAGAAAGCGGCGGGGGCCGGGACGGGTACAAGGACGACGGGGCCGGAGGCAGGGCGGGGAGATTTTGAGAAGAAGAAGAGGAGGCAGAGATATGCCTTTCAAACCAGGAGTCAAGTTGATATTCTTGATGATGGTTATAGATGGAGAAAATATGGACAAAAGGCTGTGAAGAACAACAAATTCCCAAGAAGCTACTATAGATGTACACACCAAGGTTGCAAAGTGAAGAAGCAAGTGCAAAGACTGACTAAAGATGAAGCTGTGGTTGTGACCACTTATGAAGGCATTCACTCTCATCCCATTGACAAATCCACTGATAACTTTGAGCATATTTTGACTCAGATGCAAATTTACACTTCTCAT

Protein sequence

MMEQYFPSTSLSLHQMLISRAPELAGIHGAGPLQRPHASGGFPVFRDVSIDDMSVDGVRSMKDDVVSLSRSVRFVDGSAGEGVDELEKKAAGAGTGTRTTGPEAGRGDFEKKKRRQRYAFQTRSQVDILDDGYRWRKYGQKAVKNNKFPRSYYRCTHQGCKVKKQVQRLTKDEAVVVTTYEGIHSHPIDKSTDNFEHILTQMQIYTSH

Homology

[Close]

BLAST of MS015396 vs. NCBI nr
Match: XP_022145186.1 (probable WRKY transcription factor 75 [Momordica charantia])

HSP 1 Score: 412.5 bits (1059), Expect = 2.1e-111
Identity = 205/208 (98.56%), Postives = 207/208 (99.52%), Query Frame = 0


Query: 1   MMEQYFPSTSLSLHQMLISRAPELAGIHGAGPLQRPHASGGFPVFRDVSIDDMSVDGVRS 60
           MMEQYFPSTSLSLHQMLISRAPELAGIHGAGPLQRPHASGGFPVFRDVSIDDMSVDGVRS
Sbjct: 1   MMEQYFPSTSLSLHQMLISRAPELAGIHGAGPLQRPHASGGFPVFRDVSIDDMSVDGVRS 60

Query: 61  MKDDVVSLSRSVRFVDGSAGEGVDELEKKAAGAGTGTRTTGPEAGRGDFEKKKRRQRYAF 120
           MKDDVVSLSRSVRFVDGSAGEGV+ELEKKAAGAGT TRTTGPEAGRGDFEKKKRRQRYAF
Sbjct: 61  MKDDVVSLSRSVRFVDGSAGEGVEELEKKAAGAGTSTRTTGPEAGRGDFEKKKRRQRYAF 120

Query: 121 QTRSQVDILDDGYRWRKYGQKAVKNNKFPRSYYRCTHQGCKVKKQVQRLTKDEAVVVTTY 180
           QTRSQVDILDDGYRWRKYGQKAVKNNKFPRSYYRCTHQGCKVKKQVQRLTKDEAVV+TTY
Sbjct: 121 QTRSQVDILDDGYRWRKYGQKAVKNNKFPRSYYRCTHQGCKVKKQVQRLTKDEAVVMTTY 180

Query: 181 EGIHSHPIDKSTDNFEHILTQMQIYTSH 209
           EGIHSHPIDKSTDNFEHILTQMQIYTSH
Sbjct: 181 EGIHSHPIDKSTDNFEHILTQMQIYTSH 208

[Close]

BLAST of MS015396 vs. NCBI nr
Match: XP_038902977.1 (probable WRKY transcription factor 50 [Benincasa hispida])

HSP 1 Score: 272.3 bits (695), Expect = 3.4e-69
Identity = 151/206 (73.30%), Postives = 162/206 (78.64%), Query Frame = 0


Query: 8   STSLSLHQMLISRAPELAGIHGAGPL-QRPHASGGFPVFRDVSIDDMSVDGVRSMKDD-- 67
           S+SLS HQML SRA EL G HG     Q P  SGGFP+ RDV  DDMS D   SMKDD  
Sbjct: 15  SSSLSFHQMLFSRASELVGSHGGDHYRQPPPVSGGFPLSRDVLGDDMSFDASSSMKDDDV 74

Query: 68  VVSLSRSVRFVDGSAGEGVDELEKKAAGAGTGTRTTGPEAGRGDFEKKK--RRQRYAFQT 127
           +VSLS+S R VD SA  GVD LEKK       T TTG   GRGD +KKK  R +R+AFQT
Sbjct: 75  IVSLSKSFR-VDESARIGVDGLEKKEVATAGATTTTG--TGRGDHDKKKKMRSRRFAFQT 134

Query: 128 RSQVDILDDGYRWRKYGQKAVKNNKFPRSYYRCTHQGCKVKKQVQRLTKDEAVVVTTYEG 187
           RSQVDILDDGYRWRKYGQKAVKNNKFPRSYYRCTHQGCKVKKQVQRLT+DE VVVTTYEG
Sbjct: 135 RSQVDILDDGYRWRKYGQKAVKNNKFPRSYYRCTHQGCKVKKQVQRLTRDEGVVVTTYEG 194

Query: 188 IHSHPIDKSTDNFEHILTQMQIYTSH 209
           IHSHPI+KSTDNFEHIL+QMQIYTS+
Sbjct: 195 IHSHPIEKSTDNFEHILSQMQIYTSY 217

[Close]

BLAST of MS015396 vs. NCBI nr
Match: XP_022972157.1 (probable WRKY transcription factor 75 [Cucurbita maxima])

HSP 1 Score: 243.0 bits (619), Expect = 2.2e-60
Identity = 137/215 (63.72%), Postives = 152/215 (70.70%), Query Frame = 0


Query: 1   MMEQY-------FPSTSLSLHQMLISRAPELAGIHGAGPLQRPHASGGFPVFRDVSIDDM 60
           MME Y         S SLS  + LISRA E  GIHG+   +    SGGFP+F DVS   M
Sbjct: 1   MMESYPDFLSASSSSNSLSFQKTLISRASEPTGIHGS-HFREAGISGGFPLFPDVSRSYM 60

Query: 61  SVDGVRSMKDDVVSLSRSVRFVDGSAGEGVDELEKKAAGAGTGTRTTGPEAGRGDFEKKK 120
           SVD   SMK+D+VS+S+     DGSAGE V+       G    T       G  D +KK 
Sbjct: 61  SVDTRSSMKEDMVSVSKGFH-ADGSAGEVVE-------GGAETTEMAAARQGDNDKKKKT 120

Query: 121 RRQRYAFQTRSQVDILDDGYRWRKYGQKAVKNNKFPRSYYRCTHQGCKVKKQVQRLTKDE 180
           R +R+AFQTRSQVDILDDGYRWRKYGQK VKNNKFPRSYY+CTHQGCKVKKQVQRLT+DE
Sbjct: 121 RSRRFAFQTRSQVDILDDGYRWRKYGQKGVKNNKFPRSYYKCTHQGCKVKKQVQRLTRDE 180

Query: 181 AVVVTTYEGIHSHPIDKSTDNFEHILTQMQIYTSH 209
            VVVTTYEGIHSHPI KSTDNFEHILTQMQIYTS+
Sbjct: 181 GVVVTTYEGIHSHPIQKSTDNFEHILTQMQIYTSY 206

[Close]

BLAST of MS015396 vs. NCBI nr
Match: XP_022932710.1 (probable WRKY transcription factor 75 [Cucurbita moschata])

HSP 1 Score: 239.2 bits (609), Expect = 3.2e-59
Identity = 137/215 (63.72%), Postives = 152/215 (70.70%), Query Frame = 0


Query: 1   MMEQY-------FPSTSLSLHQMLISRAPELAGIHGAGPLQRPHASGGFPVFRDVSIDDM 60
           MME Y         STSLS  +MLISRA E  GIHG+   +    SGGFP+F DVS   M
Sbjct: 1   MMESYPDFLSASSSSTSLSFQKMLISRASEPTGIHGS-HFREAGISGGFPLFPDVSGSYM 60

Query: 61  SVDGVRSMKDDVVSLSRSVRFVDGSAGEGVDELEKKAAGAGTGTRTTGPEAGRGDFEKKK 120
           SV    SMK+D+VS+S+      GSA E V+       G    T  T    G  D +KK 
Sbjct: 61  SVGTRSSMKEDMVSVSK------GSAVEVVE-------GGAETTEMTAARQGDNDKKKKT 120

Query: 121 RRQRYAFQTRSQVDILDDGYRWRKYGQKAVKNNKFPRSYYRCTHQGCKVKKQVQRLTKDE 180
           R +R+AFQTRSQVDILDDGYRWRKYGQK VKNNKFPRSYY+CTHQGCKVKKQVQRLT+DE
Sbjct: 121 RSRRFAFQTRSQVDILDDGYRWRKYGQKGVKNNKFPRSYYKCTHQGCKVKKQVQRLTRDE 180

Query: 181 AVVVTTYEGIHSHPIDKSTDNFEHILTQMQIYTSH 209
            VVVTTYEGIHSHPI KSTDNFEHILTQMQIYTS+
Sbjct: 181 GVVVTTYEGIHSHPIQKSTDNFEHILTQMQIYTSY 201

[Close]

BLAST of MS015396 vs. NCBI nr
Match: XP_023539084.1 (probable WRKY transcription factor 75 isoform X1 [Cucurbita pepo subsp. pepo] >AXF54192.1 WRKY1 [Cucurbita pepo])

HSP 1 Score: 237.3 bits (604), Expect = 1.2e-58
Identity = 136/215 (63.26%), Postives = 151/215 (70.23%), Query Frame = 0


Query: 1   MMEQY-------FPSTSLSLHQMLISRAPELAGIHGAGPLQRPHASGGFPVFRDVSIDDM 60
           MME Y         STSLS  +MLISRA E  GIHG+   +    SGGFP+F DVS   M
Sbjct: 1   MMESYPDFLSASSSSTSLSFQKMLISRASEPTGIHGS-QFREAAISGGFPLFPDVSGSYM 60

Query: 61  SVDGVRSMKDDVVSLSRSVRFVDGSAGEGVDELEKKAAGAGTGTRTTGPEAGRGDFEKKK 120
           SV    S K+D+VS+S+      GSA E V+       G    T  T    G  D +KK 
Sbjct: 61  SVGTRSSTKEDMVSVSK------GSAVEVVE-------GGAETTEMTAARQGDNDKKKKT 120

Query: 121 RRQRYAFQTRSQVDILDDGYRWRKYGQKAVKNNKFPRSYYRCTHQGCKVKKQVQRLTKDE 180
           R +R+AFQTRSQVDILDDGYRWRKYGQK VKNNKFPRSYY+CTHQGCKVKKQVQRLT+DE
Sbjct: 121 RSRRFAFQTRSQVDILDDGYRWRKYGQKGVKNNKFPRSYYKCTHQGCKVKKQVQRLTRDE 180

Query: 181 AVVVTTYEGIHSHPIDKSTDNFEHILTQMQIYTSH 209
            VVVTTYEGIHSHPI KSTDNFEHILTQMQIYTS+
Sbjct: 181 GVVVTTYEGIHSHPIQKSTDNFEHILTQMQIYTSY 201

[Close]

BLAST of MS015396 vs. ExPASy Swiss-Prot
Match: Q9FYA2 (Probable WRKY transcription factor 75 OS=Arabidopsis thaliana OX=3702 GN=WRKY75 PE=1 SV=1)

HSP 1 Score: 180.6 bits (457), Expect = 1.8e-44
Identity = 84/98 (85.71%), Postives = 93/98 (94.90%), Query Frame = 0


Query: 110 EKKKRRQRYAFQTRSQVDILDDGYRWRKYGQKAVKNNKFPRSYYRCTHQGCKVKKQVQRL 169
           +KK ++QRYAFQTRSQVDILDDGYRWRKYGQKAVKNNKFPRSYYRCT+ GC VKKQVQRL
Sbjct: 47  KKKGKKQRYAFQTRSQVDILDDGYRWRKYGQKAVKNNKFPRSYYRCTYGGCNVKKQVQRL 106

Query: 170 TKDEAVVVTTYEGIHSHPIDKSTDNFEHILTQMQIYTS 208
           T D+ VVVTTYEG+HSHPI+KST+NFEHILTQMQIY+S
Sbjct: 107 TVDQEVVVTTYEGVHSHPIEKSTENFEHILTQMQIYSS 144

[Close]

BLAST of MS015396 vs. ExPASy Swiss-Prot
Match: Q9S763 (Probable WRKY transcription factor 45 OS=Arabidopsis thaliana OX=3702 GN=WRKY45 PE=1 SV=1)

HSP 1 Score: 162.9 bits (411), Expect = 3.8e-39
Identity = 76/107 (71.03%), Postives = 88/107 (82.24%), Query Frame = 0


Query: 99  TTGPEAGRGDFEKKKRRQRYAFQTRSQVDILDDGYRWRKYGQKAVKNNKFPRSYYRCTHQ 158
           T+  E  R   +KK+R  RYAFQTRSQVDILDDGYRWRKYGQKAVKNN FPRSYY+CT +
Sbjct: 34  TSSEEKPRSKKKKKEREARYAFQTRSQVDILDDGYRWRKYGQKAVKNNPFPRSYYKCTEE 93

Query: 159 GCKVKKQVQRLTKDEAVVVTTYEGIHSHPIDKSTDNFEHILTQMQIY 206
           GC+VKKQVQR   DE VVVTTY+G+H+H +DK +DNF HILTQM I+
Sbjct: 94  GCRVKKQVQRQWGDEGVVVTTYQGVHTHAVDKPSDNFHHILTQMHIF 140

[Close]

BLAST of MS015396 vs. ExPASy Swiss-Prot
Match: Q9FFS3 (Probable WRKY transcription factor 24 OS=Arabidopsis thaliana OX=3702 GN=WRKY24 PE=1 SV=1)

HSP 1 Score: 128.3 bits (321), Expect = 1.0e-28
Identity = 63/106 (59.43%), Postives = 77/106 (72.64%), Query Frame = 0


Query: 103 EAGRGDFEKKKRR-QRYAFQTRSQVDILDDGYRWRKYGQKAVKNNKFPRSYYRCTHQGCK 162
           E G+   EK+ R+  R AF TRS  D+LDDGYRWRKYGQK+VK+N  PRSYYRCT+  C 
Sbjct: 70  EKGKELKEKRSRKVPRIAFHTRSDDDVLDDGYRWRKYGQKSVKHNAHPRSYYRCTYHTCN 129

Query: 163 VKKQVQRLTKDEAVVVTTYEGIHSHPIDKSTDNFEHILTQMQIYTS 208
           VKKQVQRL KD  VVVTTYEG+H+HP +K  +    +L Q+Q  +S
Sbjct: 130 VKKQVQRLAKDPNVVVTTYEGVHNHPCEKLMETLNPLLRQLQFLSS 175

[Close]

BLAST of MS015396 vs. ExPASy Swiss-Prot
Match: Q8VWQ4 (Probable WRKY transcription factor 56 OS=Arabidopsis thaliana OX=3702 GN=WRKY56 PE=1 SV=1)

HSP 1 Score: 128.3 bits (321), Expect = 1.0e-28
Identity = 60/98 (61.22%), Postives = 72/98 (73.47%), Query Frame = 0


Query: 106 RGDFEKKKRRQRYAFQTRSQVDILDDGYRWRKYGQKAVKNNKFPRSYYRCTHQGCKVKKQ 165
           +G  ++    QR AF TRS  D+LDDGYRWRKYGQK+VKNN  PRSYYRCT+  C VKKQ
Sbjct: 90  KGKGKRTLAMQRIAFHTRSDDDVLDDGYRWRKYGQKSVKNNAHPRSYYRCTYHTCNVKKQ 149

Query: 166 VQRLTKDEAVVVTTYEGIHSHPIDKSTDNFEHILTQMQ 204
           VQRL KD  VVVTTYEG+H+HP +K  +    +L Q+Q
Sbjct: 150 VQRLAKDPNVVVTTYEGVHNHPCEKLMETLSPLLRQLQ 187

[Close]

BLAST of MS015396 vs. ExPASy Swiss-Prot
Match: Q8GY11 (Probable WRKY transcription factor 43 OS=Arabidopsis thaliana OX=3702 GN=WRKY43 PE=1 SV=1)

HSP 1 Score: 124.4 bits (311), Expect = 1.5e-27
Identity = 55/94 (58.51%), Postives = 73/94 (77.66%), Query Frame = 0


Query: 110 EKKKRRQRYAFQTRSQVDILDDGYRWRKYGQKAVKNNKFPRSYYRCTHQGCKVKKQVQRL 169
           +KK +  R++F+T+S  DILDDGYRWRKYGQK+VKN+ +PRSYYRCT   C VKKQVQRL
Sbjct: 10  DKKMKNPRFSFRTKSDADILDDGYRWRKYGQKSVKNSLYPRSYYRCTQHMCNVKKQVQRL 69

Query: 170 TKDEAVVVTTYEGIHSHPIDKSTDNFEHILTQMQ 204
           +K+ ++V TTYEGIH+HP ++       +L Q+Q
Sbjct: 70  SKETSIVETTYEGIHNHPCEELMQTLTPLLHQLQ 103

[Close]

BLAST of MS015396 vs. ExPASy TrEMBL
Match: A0A6J1CVL3 (probable WRKY transcription factor 75 OS=Momordica charantia OX=3673 GN=LOC111014691 PE=4 SV=1)

HSP 1 Score: 412.5 bits (1059), Expect = 1.0e-111
Identity = 205/208 (98.56%), Postives = 207/208 (99.52%), Query Frame = 0


Query: 1   MMEQYFPSTSLSLHQMLISRAPELAGIHGAGPLQRPHASGGFPVFRDVSIDDMSVDGVRS 60
           MMEQYFPSTSLSLHQMLISRAPELAGIHGAGPLQRPHASGGFPVFRDVSIDDMSVDGVRS
Sbjct: 1   MMEQYFPSTSLSLHQMLISRAPELAGIHGAGPLQRPHASGGFPVFRDVSIDDMSVDGVRS 60

Query: 61  MKDDVVSLSRSVRFVDGSAGEGVDELEKKAAGAGTGTRTTGPEAGRGDFEKKKRRQRYAF 120
           MKDDVVSLSRSVRFVDGSAGEGV+ELEKKAAGAGT TRTTGPEAGRGDFEKKKRRQRYAF
Sbjct: 61  MKDDVVSLSRSVRFVDGSAGEGVEELEKKAAGAGTSTRTTGPEAGRGDFEKKKRRQRYAF 120

Query: 121 QTRSQVDILDDGYRWRKYGQKAVKNNKFPRSYYRCTHQGCKVKKQVQRLTKDEAVVVTTY 180
           QTRSQVDILDDGYRWRKYGQKAVKNNKFPRSYYRCTHQGCKVKKQVQRLTKDEAVV+TTY
Sbjct: 121 QTRSQVDILDDGYRWRKYGQKAVKNNKFPRSYYRCTHQGCKVKKQVQRLTKDEAVVMTTY 180

Query: 181 EGIHSHPIDKSTDNFEHILTQMQIYTSH 209
           EGIHSHPIDKSTDNFEHILTQMQIYTSH
Sbjct: 181 EGIHSHPIDKSTDNFEHILTQMQIYTSH 208

[Close]

BLAST of MS015396 vs. ExPASy TrEMBL
Match: A0A6J1I419 (probable WRKY transcription factor 75 OS=Cucurbita maxima OX=3661 GN=LOC111470780 PE=4 SV=1)

HSP 1 Score: 243.0 bits (619), Expect = 1.1e-60
Identity = 137/215 (63.72%), Postives = 152/215 (70.70%), Query Frame = 0


Query: 1   MMEQY-------FPSTSLSLHQMLISRAPELAGIHGAGPLQRPHASGGFPVFRDVSIDDM 60
           MME Y         S SLS  + LISRA E  GIHG+   +    SGGFP+F DVS   M
Sbjct: 1   MMESYPDFLSASSSSNSLSFQKTLISRASEPTGIHGS-HFREAGISGGFPLFPDVSRSYM 60

Query: 61  SVDGVRSMKDDVVSLSRSVRFVDGSAGEGVDELEKKAAGAGTGTRTTGPEAGRGDFEKKK 120
           SVD   SMK+D+VS+S+     DGSAGE V+       G    T       G  D +KK 
Sbjct: 61  SVDTRSSMKEDMVSVSKGFH-ADGSAGEVVE-------GGAETTEMAAARQGDNDKKKKT 120

Query: 121 RRQRYAFQTRSQVDILDDGYRWRKYGQKAVKNNKFPRSYYRCTHQGCKVKKQVQRLTKDE 180
           R +R+AFQTRSQVDILDDGYRWRKYGQK VKNNKFPRSYY+CTHQGCKVKKQVQRLT+DE
Sbjct: 121 RSRRFAFQTRSQVDILDDGYRWRKYGQKGVKNNKFPRSYYKCTHQGCKVKKQVQRLTRDE 180

Query: 181 AVVVTTYEGIHSHPIDKSTDNFEHILTQMQIYTSH 209
            VVVTTYEGIHSHPI KSTDNFEHILTQMQIYTS+
Sbjct: 181 GVVVTTYEGIHSHPIQKSTDNFEHILTQMQIYTSY 206

[Close]

BLAST of MS015396 vs. ExPASy TrEMBL
Match: A0A6J1F2W9 (probable WRKY transcription factor 75 OS=Cucurbita moschata OX=3662 GN=LOC111439177 PE=4 SV=1)

HSP 1 Score: 239.2 bits (609), Expect = 1.5e-59
Identity = 137/215 (63.72%), Postives = 152/215 (70.70%), Query Frame = 0


Query: 1   MMEQY-------FPSTSLSLHQMLISRAPELAGIHGAGPLQRPHASGGFPVFRDVSIDDM 60
           MME Y         STSLS  +MLISRA E  GIHG+   +    SGGFP+F DVS   M
Sbjct: 1   MMESYPDFLSASSSSTSLSFQKMLISRASEPTGIHGS-HFREAGISGGFPLFPDVSGSYM 60

Query: 61  SVDGVRSMKDDVVSLSRSVRFVDGSAGEGVDELEKKAAGAGTGTRTTGPEAGRGDFEKKK 120
           SV    SMK+D+VS+S+      GSA E V+       G    T  T    G  D +KK 
Sbjct: 61  SVGTRSSMKEDMVSVSK------GSAVEVVE-------GGAETTEMTAARQGDNDKKKKT 120

Query: 121 RRQRYAFQTRSQVDILDDGYRWRKYGQKAVKNNKFPRSYYRCTHQGCKVKKQVQRLTKDE 180
           R +R+AFQTRSQVDILDDGYRWRKYGQK VKNNKFPRSYY+CTHQGCKVKKQVQRLT+DE
Sbjct: 121 RSRRFAFQTRSQVDILDDGYRWRKYGQKGVKNNKFPRSYYKCTHQGCKVKKQVQRLTRDE 180

Query: 181 AVVVTTYEGIHSHPIDKSTDNFEHILTQMQIYTSH 209
            VVVTTYEGIHSHPI KSTDNFEHILTQMQIYTS+
Sbjct: 181 GVVVTTYEGIHSHPIQKSTDNFEHILTQMQIYTSY 201

[Close]

BLAST of MS015396 vs. ExPASy TrEMBL
Match: A0A345BTD5 (WRKY1 OS=Cucurbita pepo OX=3663 PE=2 SV=1)

HSP 1 Score: 237.3 bits (604), Expect = 5.9e-59
Identity = 136/215 (63.26%), Postives = 151/215 (70.23%), Query Frame = 0


Query: 1   MMEQY-------FPSTSLSLHQMLISRAPELAGIHGAGPLQRPHASGGFPVFRDVSIDDM 60
           MME Y         STSLS  +MLISRA E  GIHG+   +    SGGFP+F DVS   M
Sbjct: 1   MMESYPDFLSASSSSTSLSFQKMLISRASEPTGIHGS-QFREAAISGGFPLFPDVSGSYM 60

Query: 61  SVDGVRSMKDDVVSLSRSVRFVDGSAGEGVDELEKKAAGAGTGTRTTGPEAGRGDFEKKK 120
           SV    S K+D+VS+S+      GSA E V+       G    T  T    G  D +KK 
Sbjct: 61  SVGTRSSTKEDMVSVSK------GSAVEVVE-------GGAETTEMTAARQGDNDKKKKT 120

Query: 121 RRQRYAFQTRSQVDILDDGYRWRKYGQKAVKNNKFPRSYYRCTHQGCKVKKQVQRLTKDE 180
           R +R+AFQTRSQVDILDDGYRWRKYGQK VKNNKFPRSYY+CTHQGCKVKKQVQRLT+DE
Sbjct: 121 RSRRFAFQTRSQVDILDDGYRWRKYGQKGVKNNKFPRSYYKCTHQGCKVKKQVQRLTRDE 180

Query: 181 AVVVTTYEGIHSHPIDKSTDNFEHILTQMQIYTSH 209
            VVVTTYEGIHSHPI KSTDNFEHILTQMQIYTS+
Sbjct: 181 GVVVTTYEGIHSHPIQKSTDNFEHILTQMQIYTSY 201

[Close]

BLAST of MS015396 vs. ExPASy TrEMBL
Match: A0A5D3D1A5 (Putative WRKY transcription factor 48 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold130G001590 PE=4 SV=1)

HSP 1 Score: 236.1 bits (601), Expect = 1.3e-58
Identity = 137/215 (63.72%), Postives = 154/215 (71.63%), Query Frame = 0


Query: 8   STSLSLHQMLISRAPELAGIHGAGPLQRPHASGGFP-VFRDVSIDDMSVDGVRSMKDD-- 67
           S+SLSL Q+L SRA +  G H   P   P  S GFP +FRDVS DDMS D   S+KD+  
Sbjct: 15  SSSLSLQQILFSRASDHRGHHFRHP---PSVSDGFPMLFRDVSTDDMSFDATSSVKDEDN 74

Query: 68  ---------VVSLSRSVRFVDGSAGEGVDELEKKAAGAGTGTRTTGPEAGRGDFEKKK-- 127
                    VVS+S   R    SA +  +E  +  AGA  G      E G GD +KKK  
Sbjct: 75  NNNNNNNNVVVSVSERFRVDGSSALKKAEEEGEVRAGAMMG------ERGGGDCDKKKKM 134

Query: 128 RRQRYAFQTRSQVDILDDGYRWRKYGQKAVKNNKFPRSYYRCTHQGCKVKKQVQRLTKDE 187
           R +R+AFQTRSQVDILDDGYRWRKYGQKAVKNNKFPRSYYRCTHQGCKVKKQVQRLT+DE
Sbjct: 135 RSRRFAFQTRSQVDILDDGYRWRKYGQKAVKNNKFPRSYYRCTHQGCKVKKQVQRLTRDE 194

Query: 188 AVVVTTYEGIHSHPIDKSTDNFEHILTQMQIYTSH 209
            VVVTTYEGIHSHPI+KSTDNFEHIL+QMQIYTS+
Sbjct: 195 GVVVTTYEGIHSHPIEKSTDNFEHILSQMQIYTSY 220

[Close]

BLAST of MS015396 vs. TAIR 10
Match: AT5G13080.1 (WRKY DNA-binding protein 75 )

HSP 1 Score: 180.6 bits (457), Expect = 1.3e-45
Identity = 84/98 (85.71%), Postives = 93/98 (94.90%), Query Frame = 0


Query: 110 EKKKRRQRYAFQTRSQVDILDDGYRWRKYGQKAVKNNKFPRSYYRCTHQGCKVKKQVQRL 169
           +KK ++QRYAFQTRSQVDILDDGYRWRKYGQKAVKNNKFPRSYYRCT+ GC VKKQVQRL
Sbjct: 47  KKKGKKQRYAFQTRSQVDILDDGYRWRKYGQKAVKNNKFPRSYYRCTYGGCNVKKQVQRL 106

Query: 170 TKDEAVVVTTYEGIHSHPIDKSTDNFEHILTQMQIYTS 208
           T D+ VVVTTYEG+HSHPI+KST+NFEHILTQMQIY+S
Sbjct: 107 TVDQEVVVTTYEGVHSHPIEKSTENFEHILTQMQIYSS 144

[Close]

BLAST of MS015396 vs. TAIR 10
Match: AT3G01970.1 (WRKY DNA-binding protein 45 )

HSP 1 Score: 162.9 bits (411), Expect = 2.7e-40
Identity = 76/107 (71.03%), Postives = 88/107 (82.24%), Query Frame = 0


Query: 99  TTGPEAGRGDFEKKKRRQRYAFQTRSQVDILDDGYRWRKYGQKAVKNNKFPRSYYRCTHQ 158
           T+  E  R   +KK+R  RYAFQTRSQVDILDDGYRWRKYGQKAVKNN FPRSYY+CT +
Sbjct: 34  TSSEEKPRSKKKKKEREARYAFQTRSQVDILDDGYRWRKYGQKAVKNNPFPRSYYKCTEE 93

Query: 159 GCKVKKQVQRLTKDEAVVVTTYEGIHSHPIDKSTDNFEHILTQMQIY 206
           GC+VKKQVQR   DE VVVTTY+G+H+H +DK +DNF HILTQM I+
Sbjct: 94  GCRVKKQVQRQWGDEGVVVTTYQGVHTHAVDKPSDNFHHILTQMHIF 140

[Close]

BLAST of MS015396 vs. TAIR 10
Match: AT1G64000.1 (WRKY DNA-binding protein 56 )

HSP 1 Score: 128.3 bits (321), Expect = 7.4e-30
Identity = 60/98 (61.22%), Postives = 72/98 (73.47%), Query Frame = 0


Query: 106 RGDFEKKKRRQRYAFQTRSQVDILDDGYRWRKYGQKAVKNNKFPRSYYRCTHQGCKVKKQ 165
           +G  ++    QR AF TRS  D+LDDGYRWRKYGQK+VKNN  PRSYYRCT+  C VKKQ
Sbjct: 90  KGKGKRTLAMQRIAFHTRSDDDVLDDGYRWRKYGQKSVKNNAHPRSYYRCTYHTCNVKKQ 149

Query: 166 VQRLTKDEAVVVTTYEGIHSHPIDKSTDNFEHILTQMQ 204
           VQRL KD  VVVTTYEG+H+HP +K  +    +L Q+Q
Sbjct: 150 VQRLAKDPNVVVTTYEGVHNHPCEKLMETLSPLLRQLQ 187

[Close]

BLAST of MS015396 vs. TAIR 10
Match: AT5G41570.1 (WRKY DNA-binding protein 24 )

HSP 1 Score: 128.3 bits (321), Expect = 7.4e-30
Identity = 63/106 (59.43%), Postives = 77/106 (72.64%), Query Frame = 0


Query: 103 EAGRGDFEKKKRR-QRYAFQTRSQVDILDDGYRWRKYGQKAVKNNKFPRSYYRCTHQGCK 162
           E G+   EK+ R+  R AF TRS  D+LDDGYRWRKYGQK+VK+N  PRSYYRCT+  C 
Sbjct: 70  EKGKELKEKRSRKVPRIAFHTRSDDDVLDDGYRWRKYGQKSVKHNAHPRSYYRCTYHTCN 129

Query: 163 VKKQVQRLTKDEAVVVTTYEGIHSHPIDKSTDNFEHILTQMQIYTS 208
           VKKQVQRL KD  VVVTTYEG+H+HP +K  +    +L Q+Q  +S
Sbjct: 130 VKKQVQRLAKDPNVVVTTYEGVHNHPCEKLMETLNPLLRQLQFLSS 175

[Close]

BLAST of MS015396 vs. TAIR 10
Match: AT2G46130.1 (WRKY DNA-binding protein 43 )

HSP 1 Score: 124.4 bits (311), Expect = 1.1e-28
Identity = 55/94 (58.51%), Postives = 73/94 (77.66%), Query Frame = 0


Query: 110 EKKKRRQRYAFQTRSQVDILDDGYRWRKYGQKAVKNNKFPRSYYRCTHQGCKVKKQVQRL 169
           +KK +  R++F+T+S  DILDDGYRWRKYGQK+VKN+ +PRSYYRCT   C VKKQVQRL
Sbjct: 10  DKKMKNPRFSFRTKSDADILDDGYRWRKYGQKSVKNSLYPRSYYRCTQHMCNVKKQVQRL 69

Query: 170 TKDEAVVVTTYEGIHSHPIDKSTDNFEHILTQMQ 204
           +K+ ++V TTYEGIH+HP ++       +L Q+Q
Sbjct: 70  SKETSIVETTYEGIHNHPCEELMQTLTPLLHQLQ 103

The following BLAST results are available for this feature:

BLAST of MS015396 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022145186.1	2.1e-111	98.56	probable WRKY transcription factor 75 [Momordica charantia]	[more]
XP_038902977.1	3.4e-69	73.30	probable WRKY transcription factor 50 [Benincasa hispida]	[more]
XP_022972157.1	2.2e-60	63.72	probable WRKY transcription factor 75 [Cucurbita maxima]	[more]
XP_022932710.1	3.2e-59	63.72	probable WRKY transcription factor 75 [Cucurbita moschata]	[more]
XP_023539084.1	1.2e-58	63.26	probable WRKY transcription factor 75 isoform X1 [Cucurbita pepo subsp. pepo] >A...	[more]

BLAST of MS015396 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FYA2	1.8e-44	85.71	Probable WRKY transcription factor 75 OS=Arabidopsis thaliana OX=3702 GN=WRKY75 ...	[more]
Q9S763	3.8e-39	71.03	Probable WRKY transcription factor 45 OS=Arabidopsis thaliana OX=3702 GN=WRKY45 ...	[more]
Q9FFS3	1.0e-28	59.43	Probable WRKY transcription factor 24 OS=Arabidopsis thaliana OX=3702 GN=WRKY24 ...	[more]
Q8VWQ4	1.0e-28	61.22	Probable WRKY transcription factor 56 OS=Arabidopsis thaliana OX=3702 GN=WRKY56 ...	[more]
Q8GY11	1.5e-27	58.51	Probable WRKY transcription factor 43 OS=Arabidopsis thaliana OX=3702 GN=WRKY43 ...	[more]

BLAST of MS015396 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CVL3	1.0e-111	98.56	probable WRKY transcription factor 75 OS=Momordica charantia OX=3673 GN=LOC11101...	[more]
A0A6J1I419	1.1e-60	63.72	probable WRKY transcription factor 75 OS=Cucurbita maxima OX=3661 GN=LOC11147078...	[more]
A0A6J1F2W9	1.5e-59	63.72	probable WRKY transcription factor 75 OS=Cucurbita moschata OX=3662 GN=LOC111439...	[more]
A0A345BTD5	5.9e-59	63.26	WRKY1 OS=Cucurbita pepo OX=3663 PE=2 SV=1	[more]
A0A5D3D1A5	1.3e-58	63.72	Putative WRKY transcription factor 48 OS=Cucumis melo var. makuwa OX=1194695 GN=...	[more]

BLAST of MS015396 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G13080.1	1.3e-45	85.71	WRKY DNA-binding protein 75	[more]
AT3G01970.1	2.7e-40	71.03	WRKY DNA-binding protein 45	[more]
AT1G64000.1	7.4e-30	61.22	WRKY DNA-binding protein 56	[more]
AT5G41570.1	7.4e-30	59.43	WRKY DNA-binding protein 24	[more]
AT2G46130.1	1.1e-28	58.51	WRKY DNA-binding protein 43	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003657	WRKY domain	SMART	SM00774	WRKY_cls	coord: 129..188 e-value: 1.5E-38 score: 144.1
IPR003657	WRKY domain	PFAM	PF03106	WRKY	coord: 130..187 e-value: 4.2E-26 score: 91.0
IPR003657	WRKY domain	PROSITE	PS50811	WRKY	coord: 124..189 score: 29.746038
IPR036576	WRKY domain superfamily	GENE3D	2.20.25.80	WRKY domain	coord: 114..188 e-value: 1.8E-33 score: 116.2
IPR036576	WRKY domain superfamily	SUPERFAMILY	118290	WRKY DNA-binding domain	coord: 121..188
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 86..108
None	No IPR available	PANTHER	PTHR31221:SF222	WRKY TRANSCRIPTION FACTOR 75-RELATED	coord: 109..205
IPR044810	WRKY transcription factor, plant	PANTHER	PTHR31221	WRKY TRANSCRIPTION FACTOR PROTEIN 1-RELATED	coord: 109..205

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS015396.1	MS015396.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006355	regulation of transcription, DNA-templated
molecular_function	GO:0003700	DNA-binding transcription factor activity
molecular_function	GO:0043565	sequence-specific DNA binding