MS015356 (gene) Bitter gourd (TR) v1

Overview
NameMS015356
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionAdenine nucleotide alpha hydrolases-like superfamily protein
Locationscaffold2: 2810570 .. 2812048 (-)
RNA-Seq ExpressionMS015356
SyntenyMS015356
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTCCCCCTTCCAGAATGGCTGAAGCAGTCGAGAAGAGGGTGATGGTGGCCATAGACGAGAGCGAGTGTAGCTACTATGCCCTCATCTGGGTGCTCGAAAATCTTCAACAATCCCTAGCCGAGTCCCCCCTTTTCGTCTTCACGGCTCTTCCTCCGCCCACGATTTACACCTTCGGCGCTGGTGCTGCTGCATCGCTTGGCCTTGCGCGCACGTATTGTCACGTTACATCCAGTTAAGCATTTCTAATTTTACTTTACATACATGTAACTTCGCTAATATTTGATGTATAGAGAGAACATAACGATGATCCGTGGGCTGTCAAGTGATGAACTGAACTAATCCGGGCCTTTTAGGTCTTATGGAATTTTATTATAGTTTGAATTAGTTTGTCTGTACTTGCTGATGGATTTAAAAATAAAAAAGCATCGATTAAGACGTTGTGATGTTCTGATCAGGTCAATTTTATTGTGCACGACAGATACGGAGTTGGCTAATTCGATCCAAGAGAATGACAAGAAAGTCAGATGCGCCCTCCTTGAGAAAGCAAAGGATATATGTGCTGAAAGAGGGGTATGATTGGATTCTTACTTTCATGTCAAGTTTTCACGGTGTTCTGTATTTTGATTCAACAGGACAACTCTGACCTCCAAATTTGAACAATTTGGATCGAGAACATACGTATACTCATATTTGAAGAACTTTTTTTTAATGTTTATTTCTTGATTGCATAAATTTGATGCTACGTAAAATTAGGAATGCAAAATTGTTTATCGTCTGCAGGTGGCTGCTATATCAATCACAGAGGTTGGGAATCCTGGAACAACCATATGTGATGCAGTTGAAAAGCTCAATATAAATATGCTTGTCTTAGGTGATCGTGGCCTTGGGAGAATTAAGAGGTTGGTCTTCATTTTCTTATTTATACAACCAGTTGTGCTCAGAGGTTAGACTTTATTAATCAACATTTGCCTTCCAGAAGCGTTTAGCTTCGACATCTCAAATTTTTTCTGACGTTCGTTGTTGCCAAAACACATGCATAAGTAAGTTCATAGCTACTAGTAGATTATACTCATTCTAATGAGTAAATATCTATCATTTTCAAATGATGAAACTGAAGAGTTTTCTAGCTTATGTCCTTGCTTCTTATTAGTTGCAGAATGTCTATCAACTTATATGAAATAATTGTACATTTTTCAGCTTTTAATTTCTTACTGAGATATCCTACAACCCAGTTTGTTGCTGCCTGACTTTCAATTCGTTAGAATTTTGAGATTTTTAGCTTTCGGTATGATCACCTAATAGTACCTGTGCAACTTTTTTTCTTCTTTTTATCGATTTTGCCCCTTTTTGACTATAATGGGTTTATGTACCGGATGCTGACTGTATCAATATATGTCTCATACAGAGCTCTTATAGGGAGTGTGAGCAACTACTGTGTTCAAAATGCCAAGTGTCCTGTCCTTGTTGTGAAGAAACCA

mRNA sequence

ATTCCCCCTTCCAGAATGGCTGAAGCAGTCGAGAAGAGGGTGATGGTGGCCATAGACGAGAGCGAGTGTAGCTACTATGCCCTCATCTGGGTGCTCGAAAATCTTCAACAATCCCTAGCCGAGTCCCCCCTTTTCGTCTTCACGGCTCTTCCTCCGCCCACGATTTACACCTTCGGCGCTGGTGCTGCTGCATCGCTTGGCCTTGCGCGCACGTATTGTCACGTTACATCCAATACGGAGTTGGCTAATTCGATCCAAGAGAATGACAAGAAAGTCAGATGCGCCCTCCTTGAGAAAGCAAAGGATATATGTGCTGAAAGAGGGGTGGCTGCTATATCAATCACAGAGGTTGGGAATCCTGGAACAACCATATGTGATGCAGTTGAAAAGCTCAATATAAATATGCTTGTCTTAGGTGATCGTGGCCTTGGGAGAATTAAGAGAGCTCTTATAGGGAGTGTGAGCAACTACTGTGTTCAAAATGCCAAGTGTCCTGTCCTTGTTGTGAAGAAACCA

Coding sequence (CDS)

ATTCCCCCTTCCAGAATGGCTGAAGCAGTCGAGAAGAGGGTGATGGTGGCCATAGACGAGAGCGAGTGTAGCTACTATGCCCTCATCTGGGTGCTCGAAAATCTTCAACAATCCCTAGCCGAGTCCCCCCTTTTCGTCTTCACGGCTCTTCCTCCGCCCACGATTTACACCTTCGGCGCTGGTGCTGCTGCATCGCTTGGCCTTGCGCGCACGTATTGTCACGTTACATCCAATACGGAGTTGGCTAATTCGATCCAAGAGAATGACAAGAAAGTCAGATGCGCCCTCCTTGAGAAAGCAAAGGATATATGTGCTGAAAGAGGGGTGGCTGCTATATCAATCACAGAGGTTGGGAATCCTGGAACAACCATATGTGATGCAGTTGAAAAGCTCAATATAAATATGCTTGTCTTAGGTGATCGTGGCCTTGGGAGAATTAAGAGAGCTCTTATAGGGAGTGTGAGCAACTACTGTGTTCAAAATGCCAAGTGTCCTGTCCTTGTTGTGAAGAAACCA

Protein sequence

IPPSRMAEAVEKRVMVAIDESECSYYALIWVLENLQQSLAESPLFVFTALPPPTIYTFGAGAAASLGLARTYCHVTSNTELANSIQENDKKVRCALLEKAKDICAERGVAAISITEVGNPGTTICDAVEKLNINMLVLGDRGLGRIKRALIGSVSNYCVQNAKCPVLVVKKP
Homology
BLAST of MS015356 vs. NCBI nr
Match: XP_022150587.1 (uncharacterized protein LOC111018690 isoform X1 [Momordica charantia] >XP_022150588.1 uncharacterized protein LOC111018690 isoform X1 [Momordica charantia] >XP_022150589.1 uncharacterized protein LOC111018690 isoform X1 [Momordica charantia])

HSP 1 Score: 328.9 bits (842), Expect = 2.5e-86
Identity = 167/167 (100.00%), Postives = 167/167 (100.00%), Query Frame = 0

Query: 6   MAEAVEKRVMVAIDESECSYYALIWVLENLQQSLAESPLFVFTALPPPTIYTFGAGAAAS 65
           MAEAVEKRVMVAIDESECSYYALIWVLENLQQSLAESPLFVFTALPPPTIYTFGAGAAAS
Sbjct: 1   MAEAVEKRVMVAIDESECSYYALIWVLENLQQSLAESPLFVFTALPPPTIYTFGAGAAAS 60

Query: 66  LGLARTYCHVTSNTELANSIQENDKKVRCALLEKAKDICAERGVAAISITEVGNPGTTIC 125
           LGLARTYCHVTSNTELANSIQENDKKVRCALLEKAKDICAERGVAAISITEVGNPGTTIC
Sbjct: 61  LGLARTYCHVTSNTELANSIQENDKKVRCALLEKAKDICAERGVAAISITEVGNPGTTIC 120

Query: 126 DAVEKLNINMLVLGDRGLGRIKRALIGSVSNYCVQNAKCPVLVVKKP 173
           DAVEKLNINMLVLGDRGLGRIKRALIGSVSNYCVQNAKCPVLVVKKP
Sbjct: 121 DAVEKLNINMLVLGDRGLGRIKRALIGSVSNYCVQNAKCPVLVVKKP 167

BLAST of MS015356 vs. NCBI nr
Match: XP_022150590.1 (uncharacterized protein LOC111018690 isoform X2 [Momordica charantia])

HSP 1 Score: 280.4 bits (716), Expect = 1.0e-71
Identity = 143/143 (100.00%), Postives = 143/143 (100.00%), Query Frame = 0

Query: 6   MAEAVEKRVMVAIDESECSYYALIWVLENLQQSLAESPLFVFTALPPPTIYTFGAGAAAS 65
           MAEAVEKRVMVAIDESECSYYALIWVLENLQQSLAESPLFVFTALPPPTIYTFGAGAAAS
Sbjct: 1   MAEAVEKRVMVAIDESECSYYALIWVLENLQQSLAESPLFVFTALPPPTIYTFGAGAAAS 60

Query: 66  LGLARTYCHVTSNTELANSIQENDKKVRCALLEKAKDICAERGVAAISITEVGNPGTTIC 125
           LGLARTYCHVTSNTELANSIQENDKKVRCALLEKAKDICAERGVAAISITEVGNPGTTIC
Sbjct: 61  LGLARTYCHVTSNTELANSIQENDKKVRCALLEKAKDICAERGVAAISITEVGNPGTTIC 120

Query: 126 DAVEKLNINMLVLGDRGLGRIKR 149
           DAVEKLNINMLVLGDRGLGRIKR
Sbjct: 121 DAVEKLNINMLVLGDRGLGRIKR 143

BLAST of MS015356 vs. NCBI nr
Match: KAG6573952.1 (Acyl-acyl carrier protein thioesterase ATL3, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 276.2 bits (705), Expect = 2.0e-70
Identity = 144/177 (81.36%), Postives = 156/177 (88.14%), Query Frame = 0

Query: 2   PPSRMAEA------VEKRVMVAIDESECSYYALIWVLENLQQSLAESPLFVFTALPPPTI 61
           P + MAEA      VEKRVMVA+DESECSYYALIWVLENL+QS+A+SPLFVFTALPPPT 
Sbjct: 125 PLAEMAEAKKSNPDVEKRVMVAVDESECSYYALIWVLENLKQSIADSPLFVFTALPPPTS 184

Query: 62  YTFGAGAAASLGLARTYCHVTSNTELANSIQENDKKVRCALLEKAKDICAERGVAAISIT 121
           YT GAG  ASLGLAR+Y  V SNTELA+++QENDKKVRC +LEKAKDICAERGVAAISIT
Sbjct: 185 YTSGAG--ASLGLARSYFPVASNTELAHTLQENDKKVRCGILEKAKDICAERGVAAISIT 244

Query: 122 EVGNPGTTICDAVEKLNINMLVLGDRGLGRIKRALIGSVSNYCVQNAKCPVLVVKKP 173
           EVG PG  IC+ VEKLNIN+LVLGD GLGRIKRALIGSVSNYCVQNAKCPVLVVKKP
Sbjct: 245 EVGEPGRMICETVEKLNINLLVLGDHGLGRIKRALIGSVSNYCVQNAKCPVLVVKKP 299

BLAST of MS015356 vs. NCBI nr
Match: XP_022968136.1 (uncharacterized protein LOC111467462 [Cucurbita maxima])

HSP 1 Score: 275.4 bits (703), Expect = 3.3e-70
Identity = 143/173 (82.66%), Postives = 154/173 (89.02%), Query Frame = 0

Query: 6   MAEA------VEKRVMVAIDESECSYYALIWVLENLQQSLAESPLFVFTALPPPTIYTFG 65
           MAEA      VEKRVMVA+DESECSYYALIWVLENL+QS+A+SPLFVFTALPPPT YT G
Sbjct: 1   MAEAKKSNPDVEKRVMVAVDESECSYYALIWVLENLKQSIADSPLFVFTALPPPTSYTSG 60

Query: 66  AGAAASLGLARTYCHVTSNTELANSIQENDKKVRCALLEKAKDICAERGVAAISITEVGN 125
           AG  ASLGLAR+Y  V SNTELA+++QENDKKVRC +LEKAKDICAERGVAAISITEVG 
Sbjct: 61  AG--ASLGLARSYFPVASNTELAHTLQENDKKVRCGILEKAKDICAERGVAAISITEVGE 120

Query: 126 PGTTICDAVEKLNINMLVLGDRGLGRIKRALIGSVSNYCVQNAKCPVLVVKKP 173
           PG  ICD VEKLNIN+LVLGD G+GRIKRALIGSVSNYCVQNAKCPVLVVKKP
Sbjct: 121 PGRMICDTVEKLNINLLVLGDHGIGRIKRALIGSVSNYCVQNAKCPVLVVKKP 171

BLAST of MS015356 vs. NCBI nr
Match: XP_023541640.1 (universal stress protein A-like protein [Cucurbita pepo subsp. pepo])

HSP 1 Score: 273.9 bits (699), Expect = 9.7e-70
Identity = 140/173 (80.92%), Postives = 155/173 (89.60%), Query Frame = 0

Query: 6   MAEA------VEKRVMVAIDESECSYYALIWVLENLQQSLAESPLFVFTALPPPTIYTFG 65
           MAEA      VEKRVMVA+DESECSYYALIWVLENL+QS+ ++PLF+FTALPPPT YT G
Sbjct: 1   MAEAKKSNPDVEKRVMVAVDESECSYYALIWVLENLKQSITDAPLFIFTALPPPTSYTSG 60

Query: 66  AGAAASLGLARTYCHVTSNTELANSIQENDKKVRCALLEKAKDICAERGVAAISITEVGN 125
           AG  ASLGLAR+Y  V SNTEL++++QENDKKVRC +LEKAKDICAERGVAAISITEVG+
Sbjct: 61  AG--ASLGLARSYFPVASNTELSHTLQENDKKVRCGILEKAKDICAERGVAAISITEVGD 120

Query: 126 PGTTICDAVEKLNINMLVLGDRGLGRIKRALIGSVSNYCVQNAKCPVLVVKKP 173
           PG TICD VEKLNIN+LVLGD G+GRIKRALIGSVSNYCVQNAKCPVLVVKKP
Sbjct: 121 PGRTICDTVEKLNINLLVLGDHGIGRIKRALIGSVSNYCVQNAKCPVLVVKKP 171

BLAST of MS015356 vs. ExPASy Swiss-Prot
Match: Q8LGG8 (Universal stress protein A-like protein OS=Arabidopsis thaliana OX=3702 GN=At3g01520 PE=1 SV=2)

HSP 1 Score: 68.2 bits (165), Expect = 1.1e-10
Identity = 32/97 (32.99%), Postives = 60/97 (61.86%), Query Frame = 0

Query: 75  VTSNTELANSIQENDKKVRCALLEKAKDICAERGVAAISITEVGNPGTTICDAVEKLNIN 134
           + ++ E    +++++K     LLE   + C E GV   +  + G+P   IC  V+++  +
Sbjct: 67  IYASPEDFRDMRQSNKAKGLHLLEFFVNKCHEIGVGCEAWIKTGDPKDVICQEVKRVRPD 126

Query: 135 MLVLGDRGLGRIKRALIGSVSNYCVQNAKCPVLVVKK 172
            LV+G RGLGR ++  +G+VS +CV++A+CPV+ +K+
Sbjct: 127 FLVVGSRGLGRFQKVFVGTVSAFCVKHAECPVMTIKR 163

BLAST of MS015356 vs. ExPASy Swiss-Prot
Match: P42297 (Universal stress protein YxiE OS=Bacillus subtilis (strain 168) OX=224308 GN=yxiE PE=3 SV=1)

HSP 1 Score: 60.8 bits (146), Expect = 1.7e-08
Identity = 45/158 (28.48%), Postives = 78/158 (49.37%), Query Frame = 0

Query: 13  RVMVAIDESECSYYALIWVLENLQQSLAESPLFVFTALPPPTIYTFGAGAAASLGLARTY 72
           +++VAID S+ S  AL   +   ++  AE            +I   G  A  +       
Sbjct: 4   KMLVAIDGSDMSAKALDAAVHLAKEQQAEL-----------SILHVGREAVVTTSSLTGI 63

Query: 73  CHVTSNTELANSIQENDKKVRCALLEKAKDICAERGVAAISITEVGNPGTTICDAVEKLN 132
            +V  +    + I+   KK    +LE AK+  AE+GV A +I   G P   I +  ++  
Sbjct: 64  VYVPEH--FIDEIRNEVKKEGLKILENAKEKAAEKGVQAETIYANGEPAHEILNHAKEKG 123

Query: 133 INMLVLGDRGLGRIKRALIGSVSNYCVQNAKCPVLVVK 171
           ++++V+G RG+  +K  ++GSVS+   Q + CPVL+V+
Sbjct: 124 VSLIVVGSRGISGLKEMMLGSVSHKVSQLSTCPVLIVR 148

BLAST of MS015356 vs. ExPASy Swiss-Prot
Match: Q57951 (Universal stress protein MJ0531 OS=Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) OX=243232 GN=MJ0531 PE=3 SV=1)

HSP 1 Score: 56.2 bits (134), Expect = 4.2e-07
Identity = 30/76 (39.47%), Postives = 44/76 (57.89%), Query Frame = 0

Query: 97  LEKAKDICAERGVAAISITEVGNPGTTICDAVEKLNINMLVLGDRGLGRIKRALIGSVSN 156
           L+K K +  E GV   +    G P   I +  EK   +++V+G  G   ++R L+GSV+ 
Sbjct: 92  LKKVKKMAEEWGVKIHTEMLEGVPANEIVEFAEKKKADLIVMGTTGKTGLERILLGSVAE 151

Query: 157 YCVQNAKCPVLVVKKP 173
             ++NA CPVLVVKKP
Sbjct: 152 RVIKNAHCPVLVVKKP 167

BLAST of MS015356 vs. ExPASy Swiss-Prot
Match: P72817 (Universal stress protein Sll1654 OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=sll1654 PE=3 SV=1)

HSP 1 Score: 55.1 bits (131), Expect = 9.3e-07
Identity = 27/74 (36.49%), Postives = 45/74 (60.81%), Query Frame = 0

Query: 96  LLEKAKDICAERGVAAISITEVGNPGTTICDAVEKLNINMLVLGDRGLGRIKRALIGSVS 155
           LLE A+ + +++G+A  +I   G    TICD  +++N +++V+G RGLG     +  SV+
Sbjct: 83  LLEAAQAVFSQQGIATKTIEREGMASFTICDVADEVNADLIVMGCRGLGLTTEGVAESVT 142

Query: 156 NYCVQNAKCPVLVV 170
              +  + CPVLVV
Sbjct: 143 ARVINLSPCPVLVV 156

BLAST of MS015356 vs. ExPASy Swiss-Prot
Match: P74148 (Universal stress protein Sll1388 OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=sll1388 PE=3 SV=1)

HSP 1 Score: 54.3 bits (129), Expect = 1.6e-06
Identity = 49/167 (29.34%), Postives = 80/167 (47.90%), Query Frame = 0

Query: 13  RVMVAIDESECSYYALIWVLENLQQSLA-----ESPLFVFTALPPP----TIYTFGAGAA 72
           +++VA+D SE +        E LQQ++A      S L VF  +P      +IY    G A
Sbjct: 5   KILVALDRSELA-------KEVLQQAIALGQKESSQLMVFYCIPVDSQDLSIYPSFYGEA 64

Query: 73  ASLGLARTYCHVTSNTELANSIQENDKKVRCALLEKAKDICAERGVAAISITEVGNPGTT 132
           A +G ++          +   ++E   + R   L+       E GVA     +VG PG  
Sbjct: 65  A-IGFSQI---------IKEHLEEQQTEAR-EWLQSIVQQVQEDGVACEWDVKVGEPGRW 124

Query: 133 ICDAVEKLNINMLVLGDRGLGRIKRALIGSVSNYCVQNAKCPVLVVK 171
           I D  +  + +++VLG RGL  +    +GSVS+Y + + +C VL+V+
Sbjct: 125 IRDMAKNWDADLVVLGRRGLKGLAEVFLGSVSSYVIHHVQCSVLIVQ 153

BLAST of MS015356 vs. ExPASy TrEMBL
Match: A0A6J1D9U3 (uncharacterized protein LOC111018690 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111018690 PE=4 SV=1)

HSP 1 Score: 328.9 bits (842), Expect = 1.2e-86
Identity = 167/167 (100.00%), Postives = 167/167 (100.00%), Query Frame = 0

Query: 6   MAEAVEKRVMVAIDESECSYYALIWVLENLQQSLAESPLFVFTALPPPTIYTFGAGAAAS 65
           MAEAVEKRVMVAIDESECSYYALIWVLENLQQSLAESPLFVFTALPPPTIYTFGAGAAAS
Sbjct: 1   MAEAVEKRVMVAIDESECSYYALIWVLENLQQSLAESPLFVFTALPPPTIYTFGAGAAAS 60

Query: 66  LGLARTYCHVTSNTELANSIQENDKKVRCALLEKAKDICAERGVAAISITEVGNPGTTIC 125
           LGLARTYCHVTSNTELANSIQENDKKVRCALLEKAKDICAERGVAAISITEVGNPGTTIC
Sbjct: 61  LGLARTYCHVTSNTELANSIQENDKKVRCALLEKAKDICAERGVAAISITEVGNPGTTIC 120

Query: 126 DAVEKLNINMLVLGDRGLGRIKRALIGSVSNYCVQNAKCPVLVVKKP 173
           DAVEKLNINMLVLGDRGLGRIKRALIGSVSNYCVQNAKCPVLVVKKP
Sbjct: 121 DAVEKLNINMLVLGDRGLGRIKRALIGSVSNYCVQNAKCPVLVVKKP 167

BLAST of MS015356 vs. ExPASy TrEMBL
Match: A0A6J1D8W2 (uncharacterized protein LOC111018690 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111018690 PE=4 SV=1)

HSP 1 Score: 280.4 bits (716), Expect = 5.0e-72
Identity = 143/143 (100.00%), Postives = 143/143 (100.00%), Query Frame = 0

Query: 6   MAEAVEKRVMVAIDESECSYYALIWVLENLQQSLAESPLFVFTALPPPTIYTFGAGAAAS 65
           MAEAVEKRVMVAIDESECSYYALIWVLENLQQSLAESPLFVFTALPPPTIYTFGAGAAAS
Sbjct: 1   MAEAVEKRVMVAIDESECSYYALIWVLENLQQSLAESPLFVFTALPPPTIYTFGAGAAAS 60

Query: 66  LGLARTYCHVTSNTELANSIQENDKKVRCALLEKAKDICAERGVAAISITEVGNPGTTIC 125
           LGLARTYCHVTSNTELANSIQENDKKVRCALLEKAKDICAERGVAAISITEVGNPGTTIC
Sbjct: 61  LGLARTYCHVTSNTELANSIQENDKKVRCALLEKAKDICAERGVAAISITEVGNPGTTIC 120

Query: 126 DAVEKLNINMLVLGDRGLGRIKR 149
           DAVEKLNINMLVLGDRGLGRIKR
Sbjct: 121 DAVEKLNINMLVLGDRGLGRIKR 143

BLAST of MS015356 vs. ExPASy TrEMBL
Match: A0A6J1HU14 (uncharacterized protein LOC111467462 OS=Cucurbita maxima OX=3661 GN=LOC111467462 PE=4 SV=1)

HSP 1 Score: 275.4 bits (703), Expect = 1.6e-70
Identity = 143/173 (82.66%), Postives = 154/173 (89.02%), Query Frame = 0

Query: 6   MAEA------VEKRVMVAIDESECSYYALIWVLENLQQSLAESPLFVFTALPPPTIYTFG 65
           MAEA      VEKRVMVA+DESECSYYALIWVLENL+QS+A+SPLFVFTALPPPT YT G
Sbjct: 1   MAEAKKSNPDVEKRVMVAVDESECSYYALIWVLENLKQSIADSPLFVFTALPPPTSYTSG 60

Query: 66  AGAAASLGLARTYCHVTSNTELANSIQENDKKVRCALLEKAKDICAERGVAAISITEVGN 125
           AG  ASLGLAR+Y  V SNTELA+++QENDKKVRC +LEKAKDICAERGVAAISITEVG 
Sbjct: 61  AG--ASLGLARSYFPVASNTELAHTLQENDKKVRCGILEKAKDICAERGVAAISITEVGE 120

Query: 126 PGTTICDAVEKLNINMLVLGDRGLGRIKRALIGSVSNYCVQNAKCPVLVVKKP 173
           PG  ICD VEKLNIN+LVLGD G+GRIKRALIGSVSNYCVQNAKCPVLVVKKP
Sbjct: 121 PGRMICDTVEKLNINLLVLGDHGIGRIKRALIGSVSNYCVQNAKCPVLVVKKP 171

BLAST of MS015356 vs. ExPASy TrEMBL
Match: A0A6J1G084 (uncharacterized protein LOC111449532 OS=Cucurbita moschata OX=3662 GN=LOC111449532 PE=4 SV=1)

HSP 1 Score: 270.8 bits (691), Expect = 4.0e-69
Identity = 141/173 (81.50%), Postives = 154/173 (89.02%), Query Frame = 0

Query: 6   MAEA------VEKRVMVAIDESECSYYALIWVLENLQQSLAESPLFVFTALPPPTIYTFG 65
           MAEA      VEKRVMVA+DESECSYYALIWVLENL+QS+A+SPLF+FTALPP T YT G
Sbjct: 1   MAEAKRSNPDVEKRVMVAVDESECSYYALIWVLENLKQSIADSPLFIFTALPPRTSYTSG 60

Query: 66  AGAAASLGLARTYCHVTSNTELANSIQENDKKVRCALLEKAKDICAERGVAAISITEVGN 125
           AG  ASLGLAR+   V SNTELA+++QENDKKVRC +LEKAKDICAERGVAAISITEVG+
Sbjct: 61  AG--ASLGLARSCFPVASNTELAHTLQENDKKVRCGILEKAKDICAERGVAAISITEVGD 120

Query: 126 PGTTICDAVEKLNINMLVLGDRGLGRIKRALIGSVSNYCVQNAKCPVLVVKKP 173
           PG TICD VEKLNIN+LVLGD G+GRIKRALIGSVSNYCVQNAKCPVLVVKKP
Sbjct: 121 PGRTICDTVEKLNINLLVLGDHGIGRIKRALIGSVSNYCVQNAKCPVLVVKKP 171

BLAST of MS015356 vs. ExPASy TrEMBL
Match: A0A5A7SXH5 (Universal stress protein YxiE OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G001100 PE=4 SV=1)

HSP 1 Score: 246.1 bits (627), Expect = 1.0e-61
Identity = 129/164 (78.66%), Postives = 142/164 (86.59%), Query Frame = 0

Query: 10  VEKRVMVAIDESECSYYALIWVLENLQQSLAESPLFVFTALPPP-TIYTFGAGAAASLGL 69
           +EKRVMVAIDESE SYYALIWVLENL++S+A SPLF+FTALPPP T YT         GL
Sbjct: 1   MEKRVMVAIDESEYSYYALIWVLENLKESIASSPLFLFTALPPPSTTYT--------SGL 60

Query: 70  ARTYCHVTSNTELANSIQENDKKVRCALLEKAKDICAERGVAAISITEVGNPGTTICDAV 129
           AR+Y  + SNTE  ++IQENDKK+RC LLEKAKDICA RGVAAISITE G+PGTTICD V
Sbjct: 61  ARSYFPLPSNTEFVHTIQENDKKLRCGLLEKAKDICAGRGVAAISITEDGDPGTTICDTV 120

Query: 130 EKLNINMLVLGDRGLGRIKRALIGSVSNYCVQNAKCPVLVVKKP 173
           EKLNI++LVLGDRGLGRIKRALIGSVSNYCVQNAKCPVLVVKKP
Sbjct: 121 EKLNISLLVLGDRGLGRIKRALIGSVSNYCVQNAKCPVLVVKKP 156

BLAST of MS015356 vs. TAIR 10
Match: AT1G68300.1 (Adenine nucleotide alpha hydrolases-like superfamily protein )

HSP 1 Score: 135.6 bits (340), Expect = 3.8e-32
Identity = 78/166 (46.99%), Postives = 99/166 (59.64%), Query Frame = 0

Query: 8   EAVEKRVMVAIDESECSYYALIWVLENLQQSLAESPLFVFTALPP---PTIYTFGAGAAA 67
           ++V K+VMVAIDESECS  AL W L  L+ SLA+S + +FTA P      +Y    GAA 
Sbjct: 6   KSVMKQVMVAIDESECSKRALQWTLVYLKDSLADSDIILFTAQPHLDLSCVYASSYGAAP 65

Query: 68  SLGLARTYCHVTSNTELANSIQENDKKVRCALLEKAKDICAERGVAAISITEVGNPGTTI 127
                          EL NS+QE+ K      L++   ICAE GV    + E GNP   I
Sbjct: 66  --------------IELINSLQESHKNAGLNRLDEGTKICAETGVTPRKVLEFGNPKEAI 125

Query: 128 CDAVEKLNINMLVLGDRGLGRIKRALIGSVSNYCVQNAKCPVLVVK 171
           C+A EKL ++MLV+G  G G ++R  +GSVSNYCV NAKCPVLVV+
Sbjct: 126 CEAAEKLGVDMLVVGSHGKGALQRTFLGSVSNYCVNNAKCPVLVVR 157

BLAST of MS015356 vs. TAIR 10
Match: AT3G11930.1 (Adenine nucleotide alpha hydrolases-like superfamily protein )

HSP 1 Score: 111.3 bits (277), Expect = 7.7e-25
Identity = 60/166 (36.14%), Postives = 101/166 (60.84%), Query Frame = 0

Query: 12  KRVMVAIDESECSYYALIWVLEN-----LQQSLAESPLFVFTALPPPTIYTFGAGAAASL 71
           KR++VAIDES+ S+YAL WV+++     L  + AE+   + T +   + +   A   A  
Sbjct: 33  KRMVVAIDESDSSFYALQWVIDHFSNLLLTTAAAEAESGMLTVIHVQSPFNHFAAFPAGP 92

Query: 72  GLARTYCHVTSNTELANSIQENDKKVRCALLEKAKDICAERGVAAISITEVGNPGTTICD 131
           G A  Y    +++ +  S+++  ++   ALL +A  +C  + +   ++   G     IC+
Sbjct: 93  GGATVY----ASSSMIESVKKAQQETSAALLSRALQMCRAKQIRTETLVLEGEAKEMICE 152

Query: 132 AVEKLNINMLVLGDRGLGRIKRALIGSVSNYCVQNAKCPVLVVKKP 173
           AVEK+++++LV+G RGLG+IKRA +GSVS+YC  +A CP+L+VK P
Sbjct: 153 AVEKMHVDLLVVGSRGLGKIKRAFLGSVSDYCAHHANCPILIVKPP 194

BLAST of MS015356 vs. TAIR 10
Match: AT3G11930.4 (Adenine nucleotide alpha hydrolases-like superfamily protein )

HSP 1 Score: 110.9 bits (276), Expect = 1.0e-24
Identity = 61/168 (36.31%), Postives = 98/168 (58.33%), Query Frame = 0

Query: 12  KRVMVAIDESECSYYALIWVLENLQQSL-------AESPLFVFTALPPPTIYTFGAGAAA 71
           KR++VAIDES+ S+YAL WV+++    L       AES +     +  P    F   AA 
Sbjct: 33  KRMVVAIDESDSSFYALQWVIDHFSNLLLTTAAAEAESGMLTVIHVQSP----FNHFAAF 92

Query: 72  SLGLARTYCHVTSNTELANSIQENDKKVRCALLEKAKDICAERGVAAISITEVGNPGTTI 131
             G       V +++ +  S+++  ++   ALL +A  +C  + +   ++   G     I
Sbjct: 93  PAGPGGATAAVYASSSMIESVKKAQQETSAALLSRALQMCRAKQIRTETLVLEGEAKEMI 152

Query: 132 CDAVEKLNINMLVLGDRGLGRIKRALIGSVSNYCVQNAKCPVLVVKKP 173
           C+AVEK+++++LV+G RGLG+IKRA +GSVS+YC  +A CP+L+VK P
Sbjct: 153 CEAVEKMHVDLLVVGSRGLGKIKRAFLGSVSDYCAHHANCPILIVKPP 196

BLAST of MS015356 vs. TAIR 10
Match: AT3G11930.2 (Adenine nucleotide alpha hydrolases-like superfamily protein )

HSP 1 Score: 109.4 bits (272), Expect = 2.9e-24
Identity = 60/166 (36.14%), Postives = 101/166 (60.84%), Query Frame = 0

Query: 12  KRVMVAIDESECSYYALIWVLEN-----LQQSLAESPLFVFTALPPPTIYTFGAGAAASL 71
           KR++VAIDES+ S+YAL WV+++     L  + AE+   + T +   + +   A   A  
Sbjct: 33  KRMVVAIDESDSSFYALQWVIDHFSNLLLTTAAAEAESGMLTVIHVQSPFNHFAAFPAGP 92

Query: 72  GLARTYCHVTSNTELANSIQENDKKVRCALLEKAKDICAERGVAAISITEVGNPGTTICD 131
           G A     V +++ +  S+++  ++   ALL +A  +C  + +   ++   G     IC+
Sbjct: 93  GGATA---VYASSSMIESVKKAQQETSAALLSRALQMCRAKQIRTETLVLEGEAKEMICE 152

Query: 132 AVEKLNINMLVLGDRGLGRIKRALIGSVSNYCVQNAKCPVLVVKKP 173
           AVEK+++++LV+G RGLG+IKRA +GSVS+YC  +A CP+L+VK P
Sbjct: 153 AVEKMHVDLLVVGSRGLGKIKRAFLGSVSDYCAHHANCPILIVKPP 195

BLAST of MS015356 vs. TAIR 10
Match: AT1G09740.1 (Adenine nucleotide alpha hydrolases-like superfamily protein )

HSP 1 Score: 102.1 bits (253), Expect = 4.7e-22
Identity = 60/159 (37.74%), Postives = 92/159 (57.86%), Query Frame = 0

Query: 14  VMVAIDESECSYYALIWVLENLQ--QSLAESPLFVFTALPPPTIYTFGAGAAASLGLART 73
           V+VA+D SE S  AL W L+NL+   S ++S   V    P P++    +      G   +
Sbjct: 10  VVVAVDGSEVSMEALRWALDNLKLSSSSSDSSFVVLHVQPSPSVAAGVSPGTIPFG-GPS 69

Query: 74  YCHVTSNTELANSIQENDKKVRCALLEKAKDICAERGVAAISITEVGNPGTTICDAVEKL 133
              V + T    +I+++ K++   +LE A  ICAE+ V   +   +G+P   IC+AVE L
Sbjct: 70  GLEVPAFTA---AIEQHQKRITDTILEHASQICAEKSVNVKTQVVIGDPKYKICEAVENL 129

Query: 134 NINMLVLGDRGLGRIKRALIGSVSNYCVQNAKCPVLVVK 171
           + ++LV+G R  GRIKR  +GSVSNYC  +A CPV+++K
Sbjct: 130 HADLLVMGSRAYGRIKRMFLGSVSNYCTNHAHCPVVIIK 164

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022150587.12.5e-86100.00uncharacterized protein LOC111018690 isoform X1 [Momordica charantia] >XP_022150... [more]
XP_022150590.11.0e-71100.00uncharacterized protein LOC111018690 isoform X2 [Momordica charantia][more]
KAG6573952.12.0e-7081.36Acyl-acyl carrier protein thioesterase ATL3, chloroplastic, partial [Cucurbita a... [more]
XP_022968136.13.3e-7082.66uncharacterized protein LOC111467462 [Cucurbita maxima][more]
XP_023541640.19.7e-7080.92universal stress protein A-like protein [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q8LGG81.1e-1032.99Universal stress protein A-like protein OS=Arabidopsis thaliana OX=3702 GN=At3g0... [more]
P422971.7e-0828.48Universal stress protein YxiE OS=Bacillus subtilis (strain 168) OX=224308 GN=yxi... [more]
Q579514.2e-0739.47Universal stress protein MJ0531 OS=Methanocaldococcus jannaschii (strain ATCC 43... [more]
P728179.3e-0736.49Universal stress protein Sll1654 OS=Synechocystis sp. (strain PCC 6803 / Kazusa)... [more]
P741481.6e-0629.34Universal stress protein Sll1388 OS=Synechocystis sp. (strain PCC 6803 / Kazusa)... [more]
Match NameE-valueIdentityDescription
A0A6J1D9U31.2e-86100.00uncharacterized protein LOC111018690 isoform X1 OS=Momordica charantia OX=3673 G... [more]
A0A6J1D8W25.0e-72100.00uncharacterized protein LOC111018690 isoform X2 OS=Momordica charantia OX=3673 G... [more]
A0A6J1HU141.6e-7082.66uncharacterized protein LOC111467462 OS=Cucurbita maxima OX=3661 GN=LOC111467462... [more]
A0A6J1G0844.0e-6981.50uncharacterized protein LOC111449532 OS=Cucurbita moschata OX=3662 GN=LOC1114495... [more]
A0A5A7SXH51.0e-6178.66Universal stress protein YxiE OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc... [more]
Match NameE-valueIdentityDescription
AT1G68300.13.8e-3246.99Adenine nucleotide alpha hydrolases-like superfamily protein [more]
AT3G11930.17.7e-2536.14Adenine nucleotide alpha hydrolases-like superfamily protein [more]
AT3G11930.41.0e-2436.31Adenine nucleotide alpha hydrolases-like superfamily protein [more]
AT3G11930.22.9e-2436.14Adenine nucleotide alpha hydrolases-like superfamily protein [more]
AT1G09740.14.7e-2237.74Adenine nucleotide alpha hydrolases-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006015Universal stress protein A familyPRINTSPR01438UNVRSLSTRESScoord: 148..170
score: 41.39
coord: 11..29
score: 30.32
coord: 130..142
score: 27.38
IPR006016UspAPFAMPF00582Uspcoord: 12..170
e-value: 5.5E-29
score: 101.5
IPR014729Rossmann-like alpha/beta/alpha sandwich foldGENE3D3.40.50.620HUPscoord: 4..172
e-value: 3.2E-39
score: 137.0
NoneNo IPR availablePANTHERPTHR31964:SF55UNIVERSAL STRESS PROTEIN A-LIKE PROTEINcoord: 7..172
NoneNo IPR availablePANTHERPTHR31964ADENINE NUCLEOTIDE ALPHA HYDROLASES-LIKE SUPERFAMILY PROTEINcoord: 7..172
NoneNo IPR availableCDDcd00293USP_Likecoord: 13..169
e-value: 4.4883E-31
score: 107.456
NoneNo IPR availableSUPERFAMILY52402Adenine nucleotide alpha hydrolases-likecoord: 12..171

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS015356.1MS015356.1mRNA