MS015308 (gene) Bitter gourd (TR) v1

Overview
NameMS015308
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
Descriptiontranscription factor bHLH30-like
Locationscaffold2: 2471628 .. 2474419 (+)
RNA-Seq ExpressionMS015308
SyntenyMS015308
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
TGCTCACAAACAATAGAGAATATGTTTCAAGAACAGCTTCTTCTCCAACATCAGCTCCAAAACAATACCAACGATAATATTATATATGGAGATCATCATCAGCATCATCTTCATCATGGAGTACTTGGAGGTGGTGGAAGAGGGTTGATTTTCCCGGCAGAAGTAATCTCGCCGGTTCTCCACCCGCCGTGGCCACCGCTCCCGGTCCGACAGCACAACCCATTTCTGGTCCCCGCTCCTCTTCCTCCTCCTCGCCCATCATCCGCGGGATATTCGAACTTAAACTAACAGCACATACCGATCCAGGAAAAATATAAAATAACGACTAACTAAGCTTCAACCATAAATTCGACAAATAAGTGAATTTTTTCTCTCTTTCTCATTTATTAGTTGAATTTATTGTCGAAGTTTAGTTAGTTGTTATTCTATATTTTTCTGGATTTTGGTGCGCTGTTAGTTTAAGTATGAATACTCGGCATCATCTTCATCATTTGGTAGTTTGTTTAACCGGAGACGTACTAATTGTGTGCAGTTTGGTTACGACGGTCCATCGGCGGCGGACCACCTGGGGCGGATCATATCGAGCACGCTTGGACCGGTGGGTTCGGGAGCTCCGTTTGGGCTACAAGCGGAGCTTGGGAAGATGAGTGCTCAAGAAATTATGGATGCTAAAGCTCTGGCAGCTTCTAAAAGCCATAGTGAAGCTGAGCGGAGAAGAAGAGAAAGAATCAATAACCATCTGGCTAAGCTCCGTAGTTTGCTTCCAAGTACCACTAAAGTAAGCACCTTTTTCTTCTCTATTAAATTCCCATATTTCATATAACCATTTATTCATGGGCTTTCACTCTTTGGAACAACACCATGCTCTCCATTCTTTTATCTTATTGATTCTCACATACCCTTTGGATCTAAGTTACAATCTTTGAATCATATACAAATACAAATTCCTTGCATAATATATCCAAATCTTAGAAATTTCTAAGATTTCATTGTCAACTTTTGGGTCTATACCTGGTTTCACCCAAAAAGATGAAAATGTTATCCTAGTGAATAATGTTTCCTACTCACCACTAAAAATTTGTCCGCTATTTACTTTCTTCAATTCTCTTTCTCCATTTCTCTCTCCTACCTGTCAAAATTTTAATAGTCCACAAAAAAAAGTATTTTTCTATATCCGCCTAGTTCATAGTTTCAAGCTAAGGAGAGGGATATTGTTAGAATTACTCATAGTTCTTACAACTTCGTGGAAGAATATCAAAGGATATGTTTTGTTTTTGTCTTGAAACATCTTGTAGTTAGGAAGGACGGAGGGGGAAGAAGAAGAAGAGGAAAGGAGAAAAAGAAAATAACCGTTAATGTCGCTGGATTGATATGATAAAGAGGAACCAAAGTTGCAGATGGAAAGGCTTTTCGGTTGGGAAATTTGTTGCCGGATGTCGGTCAAAAAAAACTACGTTAGAGAAATCAAAAGAAATAAAGACAGACAAATTCAGCATTCTTGTCAGTCACCCCCCCTTAGACTAGAGACACCCTTCTCAACTCAGCCCCACTACCAATGAACCTAAACCCAAAACACATCCCCCACTACTAACTAATTACCCAATGCCCCAAAACTTAGAATTTTCTTGGTGGATTATTTTGTACTCCCTTAGTAATAAACAATGTTTCAAAAAAAAAAAAAAATTAAAACTTGAATGAAAGAATAAGAAATTATTTGAAATGTATAAGAAGTAGTTAAAATATGGAGATAAATTTTCAACTTAACATCTAACCCTTATTAAGATAGTTACTTTTAAGAGTCTGTTCAAGGGAGCCCTCTGACAATTGAAAGATGATAAGGAATCTTAGAAAAGATCGATTGAGACTTCTTCATCACTACGTGTTTGTCCAGTCTAGGACAGCAGAGATCTTCCAATCTACGTGCGTGCGAAGAGTCTGGTCTGAATTTAGGTCTAGTTTGCCCGTCTGTTGCTAGGTTCGGGAATGGTGCCAGGCGGGGCGCTGTCATGCCCAACAGCTCTACTACTACGACCTTCACTTGATTTGGATGGTTGGCATAGATCTCATGAGACGCAAACGACCATATTAACTTACATGCCTATCCCAAAACTTTCTTACTTTTAGCGGTTCATAAATATTAGTGAGATTCATTTACATATGATTTTATGTCTCATTTGTTATGTTGTTTTTGTTTTTTGTTTTTTTATACCCAATTTAAGCCTAATGGTAGTAGTAGTAGAATAGGGTAAGCTATATATGTTGATAAAAGCAAAAGTAAAAGGAGATGAAGTTAATTAAAAAGGAAGGCATTGGGGGGATGGTGAGGTTTTTTTTTTGTTCATGTACAGACGGACAAAGCTTCATTACTAGCAGAAGTAATACAGCACGTGAAGGAGCTAAAGCGCCAAACGTGTGCAATAGCGGAAACAAGCCCAATTCCCAGCGAAGCCGACGAGCTAACTGTCGATGACGCCTCCGATGAGAGCGGTGGCGGCAAGTTCGTAATAAAGGCCTCTCTTTGCTGCGACGACCGCTCCGACCTCCTCCCCGACCTCATCAAAACACTCAAATCTCTGCGTTTACGGACGCTAAAAGCTGAGATCACCACCCTCGGCGGTCGCGTTAGAAATGTCTTGTTCGTCACCGGAGACGACAACGACAGCAGCAGCAACAATAGCATAAGCTCTATTCAAGAAGCACTCAAGGCTGTCATGGAGAAGCCCGGAGGAGGGGATGATTCTTCTTCCGGGAATATTAAAAGACAAAGGACAAATAACAACATCAATATCCTT

mRNA sequence

TGCTCACAAACAATAGAGAATATGTTTCAAGAACAGCTTCTTCTCCAACATCAGCTCCAAAACAATACCAACGATAATATTATATATGGAGATCATCATCAGCATCATCTTCATCATGGAGTACTTGGAGGTGGTGGAAGAGGGTTGATTTTCCCGGCAGAAGTAATCTCGCCGGTTCTCCACCCGCCGTGGCCACCGCTCCCGTTTAAGTATGAATACTCGGCATCATCTTCATCATTTGGTAGTTTGTTTAACCGGAGACGTACTAATTGTGTGCAGTTTGGTTACGACGGTCCATCGGCGGCGGACCACCTGGGGCGGATCATATCGAGCACGCTTGGACCGGTGGGTTCGGGAGCTCCGTTTGGGCTACAAGCGGAGCTTGGGAAGATGAGTGCTCAAGAAATTATGGATGCTAAAGCTCTGGCAGCTTCTAAAAGCCATAGTGAAGCTGAGCGGAGAAGAAGAGAAAGAATCAATAACCATCTGGCTAAGCTCCGTAGTTTGCTTCCAAGTACCACTAAAACGGACAAAGCTTCATTACTAGCAGAAGTAATACAGCACGTGAAGGAGCTAAAGCGCCAAACGTGTGCAATAGCGGAAACAAGCCCAATTCCCAGCGAAGCCGACGAGCTAACTGTCGATGACGCCTCCGATGAGAGCGGTGGCGGCAAGTTCGTAATAAAGGCCTCTCTTTGCTGCGACGACCGCTCCGACCTCCTCCCCGACCTCATCAAAACACTCAAATCTCTGCGTTTACGGACGCTAAAAGCTGAGATCACCACCCTCGGCGGTCGCGTTAGAAATGTCTTGTTCGTCACCGGAGACGACAACGACAGCAGCAGCAACAATAGCATAAGCTCTATTCAAGAAGCACTCAAGGCTGTCATGGAGAAGCCCGGAGGAGGGGATGATTCTTCTTCCGGGAATATTAAAAGACAAAGGACAAATAACAACATCAATATCCTT

Coding sequence (CDS)

TGCTCACAAACAATAGAGAATATGTTTCAAGAACAGCTTCTTCTCCAACATCAGCTCCAAAACAATACCAACGATAATATTATATATGGAGATCATCATCAGCATCATCTTCATCATGGAGTACTTGGAGGTGGTGGAAGAGGGTTGATTTTCCCGGCAGAAGTAATCTCGCCGGTTCTCCACCCGCCGTGGCCACCGCTCCCGTTTAAGTATGAATACTCGGCATCATCTTCATCATTTGGTAGTTTGTTTAACCGGAGACGTACTAATTGTGTGCAGTTTGGTTACGACGGTCCATCGGCGGCGGACCACCTGGGGCGGATCATATCGAGCACGCTTGGACCGGTGGGTTCGGGAGCTCCGTTTGGGCTACAAGCGGAGCTTGGGAAGATGAGTGCTCAAGAAATTATGGATGCTAAAGCTCTGGCAGCTTCTAAAAGCCATAGTGAAGCTGAGCGGAGAAGAAGAGAAAGAATCAATAACCATCTGGCTAAGCTCCGTAGTTTGCTTCCAAGTACCACTAAAACGGACAAAGCTTCATTACTAGCAGAAGTAATACAGCACGTGAAGGAGCTAAAGCGCCAAACGTGTGCAATAGCGGAAACAAGCCCAATTCCCAGCGAAGCCGACGAGCTAACTGTCGATGACGCCTCCGATGAGAGCGGTGGCGGCAAGTTCGTAATAAAGGCCTCTCTTTGCTGCGACGACCGCTCCGACCTCCTCCCCGACCTCATCAAAACACTCAAATCTCTGCGTTTACGGACGCTAAAAGCTGAGATCACCACCCTCGGCGGTCGCGTTAGAAATGTCTTGTTCGTCACCGGAGACGACAACGACAGCAGCAGCAACAATAGCATAAGCTCTATTCAAGAAGCACTCAAGGCTGTCATGGAGAAGCCCGGAGGAGGGGATGATTCTTCTTCCGGGAATATTAAAAGACAAAGGACAAATAACAACATCAATATCCTT

Protein sequence

CSQTIENMFQEQLLLQHQLQNNTNDNIIYGDHHQHHLHHGVLGGGGRGLIFPAEVISPVLHPPWPPLPFKYEYSASSSSFGSLFNRRRTNCVQFGYDGPSAADHLGRIISSTLGPVGSGAPFGLQAELGKMSAQEIMDAKALAASKSHSEAERRRRERINNHLAKLRSLLPSTTKTDKASLLAEVIQHVKELKRQTCAIAETSPIPSEADELTVDDASDESGGGKFVIKASLCCDDRSDLLPDLIKTLKSLRLRTLKAEITTLGGRVRNVLFVTGDDNDSSSNNSISSIQEALKAVMEKPGGGDDSSSGNIKRQRTNNNINIL
Homology
BLAST of MS015308 vs. NCBI nr
Match: XP_022150481.1 (transcription factor bHLH30-like [Momordica charantia])

HSP 1 Score: 560.1 bits (1442), Expect = 1.3e-155
Identity = 298/323 (92.26%), Postives = 299/323 (92.57%), Query Frame = 0

Query: 1   CSQTIENMFQEQLLLQHQLQNNTNDNIIYGDHHQHHLHHGVLGGGGRGLIFPAEVISPVL 60
           CSQTIENMFQEQLLLQHQLQNNTNDNIIYGDHHQHHLHHGVLGGGGRGLIFPAEVISPVL
Sbjct: 19  CSQTIENMFQEQLLLQHQLQNNTNDNIIYGDHHQHHLHHGVLGGGGRGLIFPAEVISPVL 78

Query: 61  HPPWPPLPFKYEYSASSSSFGSLFNRRRTNCVQFGYDGPSAADHLGRIISSTLGPVGSGA 120
           HPPWPPLP                  R+ N   FGYDGPSAADHLGRIISSTLGPVGSGA
Sbjct: 79  HPPWPPLPV-----------------RQHNPFLFGYDGPSAADHLGRIISSTLGPVGSGA 138

Query: 121 PFGLQAELGKMSAQEIMDAKALAASKSHSEAERRRRERINNHLAKLRSLLPSTTKTDKAS 180
           PFGLQAELGKMSAQEIMDAKALAASKSHSEAERRRRERINNHLAKLRSLLPSTTKTDKAS
Sbjct: 139 PFGLQAELGKMSAQEIMDAKALAASKSHSEAERRRRERINNHLAKLRSLLPSTTKTDKAS 198

Query: 181 LLAEVIQHVKELKRQTCAIAETSPIPSEADELTVDDASDESGGGKFVIKASLCCDDRSDL 240
           LLAEVIQHVKELKRQTCAIAETSPIPSEADELTVDDASDESGGGKFVIKASLCCDDRSDL
Sbjct: 199 LLAEVIQHVKELKRQTCAIAETSPIPSEADELTVDDASDESGGGKFVIKASLCCDDRSDL 258

Query: 241 LPDLIKTLKSLRLRTLKAEITTLGGRVRNVLFVTGDDNDSSSNNSISSIQEALKAVMEKP 300
           LPDLIKTLKSLRLRTLKAEITTLGGRVRNVLFVT DD DSSSNNSISSIQEALKAVMEKP
Sbjct: 259 LPDLIKTLKSLRLRTLKAEITTLGGRVRNVLFVTRDDEDSSSNNSISSIQEALKAVMEKP 318

Query: 301 GGGDDSSSGNIKRQRTNNNINIL 324
           GGGDDSSSGNIKRQRTNNNINIL
Sbjct: 319 GGGDDSSSGNIKRQRTNNNINIL 324

BLAST of MS015308 vs. NCBI nr
Match: XP_022945399.1 (transcription factor bHLH30-like [Cucurbita moschata])

HSP 1 Score: 456.1 bits (1172), Expect = 2.6e-124
Identity = 270/361 (74.79%), Postives = 286/361 (79.22%), Query Frame = 0

Query: 1   CSQTIENMFQEQLLLQHQLQNNTNDNIIYGDHHQHHLHHGVLGGGGRGLIFPAEVISPVL 60
           CSQTIENMFQEQLLL  QLQNNT D+IIYGDHH H        G GRGLIFPA+V+SPVL
Sbjct: 18  CSQTIENMFQEQLLLHQQLQNNTTDHIIYGDHHHH--------GAGRGLIFPADVVSPVL 77

Query: 61  HPPWPPL----------PFKYEYSASSSSFGSLFNRRRTNCVQFGYDGPSAADHLGRIIS 120
           +  WPPL          P       SSSS+G+LFNRR  NC+QF YDG S+ADHLGRIIS
Sbjct: 78  N-SWPPLNPFLIPPPPPPPPPTSLCSSSSYGNLFNRRPANCLQFAYDGTSSADHLGRIIS 137

Query: 121 STLGPV---GSGAPFGLQAELGKMSAQEIMDAKALAASKSHSEAERRRRERINNHLAKLR 180
           +TLGPV   GS APFGLQAELGKMSAQEIMDAKALAASKSHSEAERRRRERINNHLAKLR
Sbjct: 138 TTLGPVVHHGSTAPFGLQAELGKMSAQEIMDAKALAASKSHSEAERRRRERINNHLAKLR 197

Query: 181 SLLPSTTKTDKASLLAEVIQHVKELKRQTCAIAETSPIPSEADELTVDDASDES--GGGK 240
           SLLPSTTKTDKASLLAEVIQHVKELKRQT  IAETSPIP++ DELTVDDASDE    G K
Sbjct: 198 SLLPSTTKTDKASLLAEVIQHVKELKRQTSLIAETSPIPTDIDELTVDDASDEMMINGAK 257

Query: 241 FVIKASLCCDDRSDLLPDLIKTLKSLRLRTLKAEITTLGGRVRNVLFVTGDD-------- 300
           FVIKASLCC+DRSDLLPDLIKTLKSLRLRTLKAEITTLGGRVRNVLFVTG+D        
Sbjct: 258 FVIKASLCCEDRSDLLPDLIKTLKSLRLRTLKAEITTLGGRVRNVLFVTGEDDEPSTDHN 317

Query: 301 -NDSSSNN--------------SISSIQEALKAVMEKPGGGDDSSSGNIKRQRTNNNINI 324
            ND+++NN              SISSIQEALKAVMEK  G DDSSSGNIKRQRT NNINI
Sbjct: 318 NNDNNNNNNNPNSDSQQQQQQHSISSIQEALKAVMEKT-GPDDSSSGNIKRQRT-NNINI 367

BLAST of MS015308 vs. NCBI nr
Match: KAG7013054.1 (Transcription factor bHLH30, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 454.5 bits (1168), Expect = 7.5e-124
Identity = 269/360 (74.72%), Postives = 286/360 (79.44%), Query Frame = 0

Query: 1   CSQTIENMFQEQLLLQHQLQNNTNDNIIYGDHHQHHLHHGVLGGGGRGLIFPAEVISPVL 60
           CSQTIENMFQEQLLL  QLQNNT D+IIYGDHH H        G GRGLIFPA+V+SPVL
Sbjct: 19  CSQTIENMFQEQLLLHQQLQNNTTDHIIYGDHHHH--------GAGRGLIFPADVVSPVL 78

Query: 61  HPPWPPL----------PFKYEYSASSSSFGSLFNRRRTNCVQFGYDGPSAADHLGRIIS 120
           +  WPPL          P       SSSS+G+LFNRR  NC+QF YDG S+ADHLGRIIS
Sbjct: 79  N-SWPPLNPFLIPPPPPPPPPTSLCSSSSYGNLFNRRPANCLQFAYDGTSSADHLGRIIS 138

Query: 121 STLGPV---GSGAPFGLQAELGKMSAQEIMDAKALAASKSHSEAERRRRERINNHLAKLR 180
           +TLGPV   GS APFGLQAELGKMSAQEIMDAKALAASKSHSEAERRRRERINNHLAKLR
Sbjct: 139 TTLGPVVHHGSTAPFGLQAELGKMSAQEIMDAKALAASKSHSEAERRRRERINNHLAKLR 198

Query: 181 SLLPSTTKTDKASLLAEVIQHVKELKRQTCAIAETSPIPSEADELTVDDASDES--GGGK 240
           SLLPSTTKTDKASLLAEVIQHVKELKRQT  IAETSPIP++ DELTVDDASDE    G K
Sbjct: 199 SLLPSTTKTDKASLLAEVIQHVKELKRQTSLIAETSPIPTDIDELTVDDASDEMMINGAK 258

Query: 241 FVIKASLCCDDRSDLLPDLIKTLKSLRLRTLKAEITTLGGRVRNVLFVTGDD-------- 300
           FVIKASLCC+DRSDLLPDLIKTLKSLRLRTLKAEITTLGGRVRNVLFVTG+D        
Sbjct: 259 FVIKASLCCEDRSDLLPDLIKTLKSLRLRTLKAEITTLGGRVRNVLFVTGEDDEPSTDHN 318

Query: 301 ---NDSSSNN-----------SISSIQEALKAVMEKPGGGDDSSSGNIKRQRTNNNINIL 324
              N++++NN           SISSIQEALKAVMEK  G DDSSSGNIKRQRT NNINIL
Sbjct: 319 NNNNNNNNNNPNSDSQQQQQHSISSIQEALKAVMEKT-GPDDSSSGNIKRQRT-NNINIL 367

BLAST of MS015308 vs. NCBI nr
Match: KAG6573995.1 (Transcription factor basic helix-loop-helix 30, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 454.1 bits (1167), Expect = 9.8e-124
Identity = 269/361 (74.52%), Postives = 286/361 (79.22%), Query Frame = 0

Query: 1   CSQTIENMFQEQLLLQHQLQNNTNDNIIYGDHHQHHLHHGVLGGGGRGLIFPAEVISPVL 60
           CSQTIENMFQEQLLL  QLQNNT D+IIYGDHH H        G GRGLIFPA+V+SPVL
Sbjct: 19  CSQTIENMFQEQLLLHQQLQNNTTDHIIYGDHHHH--------GAGRGLIFPADVVSPVL 78

Query: 61  HPPWPPL----------PFKYEYSASSSSFGSLFNRRRTNCVQFGYDGPSAADHLGRIIS 120
           +  WPPL          P       SSSS+G+LFNRR  NC+QF YDG S+ADHLGRIIS
Sbjct: 79  N-SWPPLNPFLIPPPPPPPPPTSLCSSSSYGNLFNRRPANCLQFAYDGTSSADHLGRIIS 138

Query: 121 STLGPV---GSGAPFGLQAELGKMSAQEIMDAKALAASKSHSEAERRRRERINNHLAKLR 180
           +TLGPV   GS APFGLQAELGKMSAQEIMDAKALAASKSHSEAERRRRERINNHLAKLR
Sbjct: 139 TTLGPVVHHGSTAPFGLQAELGKMSAQEIMDAKALAASKSHSEAERRRRERINNHLAKLR 198

Query: 181 SLLPSTTKTDKASLLAEVIQHVKELKRQTCAIAETSPIPSEADELTVDDASDES--GGGK 240
           SLLPSTTKTDKASLLAEVIQHVKELKRQT  IAETSPIP++ DELTVDDASDE    G K
Sbjct: 199 SLLPSTTKTDKASLLAEVIQHVKELKRQTSLIAETSPIPTDIDELTVDDASDEMMINGAK 258

Query: 241 FVIKASLCCDDRSDLLPDLIKTLKSLRLRTLKAEITTLGGRVRNVLFVTGDD-------- 300
           FVIKASLCC+DRSDLLPDLIKTLKSLRLRTLKAEITTLGGRVRNVLFVTG+D        
Sbjct: 259 FVIKASLCCEDRSDLLPDLIKTLKSLRLRTLKAEITTLGGRVRNVLFVTGEDDEPSTDHN 318

Query: 301 ---NDSSSNN------------SISSIQEALKAVMEKPGGGDDSSSGNIKRQRTNNNINI 324
              N++++NN            SISSIQEALKAVMEK  G DDSSSGNIKRQRT NNINI
Sbjct: 319 NNNNNNNNNNPNSDSQQQQQQHSISSIQEALKAVMEKT-GPDDSSSGNIKRQRT-NNINI 368

BLAST of MS015308 vs. NCBI nr
Match: XP_023542825.1 (transcription factor bHLH30-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 453.4 bits (1165), Expect = 1.7e-123
Identity = 268/360 (74.44%), Postives = 285/360 (79.17%), Query Frame = 0

Query: 1   CSQTIENMFQEQLLLQHQLQNNTNDNIIYGDHHQHHLHHGVLGGGGRGLIFPAEVISPVL 60
           CSQTIENMFQEQLLL  QLQNN  D+IIYGDHH H        G GRGLIFPA+V+SPVL
Sbjct: 18  CSQTIENMFQEQLLLHQQLQNNNTDHIIYGDHHHH--------GAGRGLIFPADVVSPVL 77

Query: 61  HPPWPPL----------PFKYEYSASSSSFGSLFNRRRTNCVQFGYDGPSAADHLGRIIS 120
           +  WPPL          P       SSSS+G+LFNRR  NC+QF YDG S+ADHLGRIIS
Sbjct: 78  N-SWPPLNPFLIPPPPPPPPPTSLCSSSSYGNLFNRRPANCLQFAYDGTSSADHLGRIIS 137

Query: 121 STLGPV---GSGAPFGLQAELGKMSAQEIMDAKALAASKSHSEAERRRRERINNHLAKLR 180
           +TLGPV   GS APFGLQAELGKMSAQEIMDAKALAASKSHSEAERRRRERINNHLAKLR
Sbjct: 138 TTLGPVVHHGSAAPFGLQAELGKMSAQEIMDAKALAASKSHSEAERRRRERINNHLAKLR 197

Query: 181 SLLPSTTKTDKASLLAEVIQHVKELKRQTCAIAETSPIPSEADELTVDDASDES--GGGK 240
           SLLPSTTKTDKASLLAEVIQHVKELKRQT  IAETSPIP++ DELTVDDASDE    G K
Sbjct: 198 SLLPSTTKTDKASLLAEVIQHVKELKRQTSLIAETSPIPTDIDELTVDDASDEMMINGAK 257

Query: 241 FVIKASLCCDDRSDLLPDLIKTLKSLRLRTLKAEITTLGGRVRNVLFVTGDD-------- 300
           FVIKASLCC+DRSDLLPDLIKTLKSLRLRTLKAEITTLGGRVRNVLFVTG+D        
Sbjct: 258 FVIKASLCCEDRSDLLPDLIKTLKSLRLRTLKAEITTLGGRVRNVLFVTGEDDEPSTDHN 317

Query: 301 ---NDSSSNN-----------SISSIQEALKAVMEKPGGGDDSSSGNIKRQRTNNNINIL 324
              N++++NN           SISSIQEALKAVMEK  G DDSSSGNIKRQRT NNINIL
Sbjct: 318 NNNNNNNNNNPNSDTQQQQQHSISSIQEALKAVMEKT-GPDDSSSGNIKRQRT-NNINIL 366

BLAST of MS015308 vs. ExPASy Swiss-Prot
Match: Q9S7Y1 (Transcription factor bHLH30 OS=Arabidopsis thaliana OX=3702 GN=BHLH30 PE=1 SV=1)

HSP 1 Score: 309.7 bits (792), Expect = 3.9e-83
Identity = 201/341 (58.94%), Postives = 243/341 (71.26%), Query Frame = 0

Query: 17  HQLQNNTNDNIIYG--DHHQHHLHH-------GVLGGGGRGL-IFPAEVISPV--LHPP- 76
           + +QN  ND   +    HH HH H        G  G  G G  IF  + +SP+  L PP 
Sbjct: 20  NNIQNYQNDLFFHQLISHHHHHHHDPSQSETLGASGNVGSGFTIFSQDSVSPIWSLPPPT 79

Query: 77  --WPPL-PFKYEYSASSSSFGSLFNRRRTN--CVQFGYDG----PSAADHLG---RIISS 136
              PP   F    S+ +S +GS FNR R +   +QFGY+G     SAA H     RI+S 
Sbjct: 80  SIQPPFDQFPPPSSSPASFYGSFFNRSRAHHQGLQFGYEGFGGATSAAHHHHEQLRILSE 139

Query: 137 TLGPV---GSGAPFGLQAELGKMSAQEIMDAKALAASKSHSEAERRRRERINNHLAKLRS 196
            LGPV   GSG PFGLQAELGKM+AQEIMDAKALAASKSHSEAERRRRERINNHLAKLRS
Sbjct: 140 ALGPVVQAGSG-PFGLQAELGKMTAQEIMDAKALAASKSHSEAERRRRERINNHLAKLRS 199

Query: 197 LLPSTTKTDKASLLAEVIQHVKELKRQTCAIAETSPIPSEADELTVD-DASDESGGGKFV 256
           +LP+TTKTDKASLLAEVIQHVKELKR+T  I+ET+ +P+E+DELTV     +E+G G+FV
Sbjct: 200 ILPNTTKTDKASLLAEVIQHVKELKRETSVISETNLVPTESDELTVAFTEEEETGDGRFV 259

Query: 257 IKASLCCDDRSDLLPDLIKTLKSLRLRTLKAEITTLGGRVRNVLFVTGDDN---DSSSNN 316
           IKASLCC+DRSDLLPD+IKTLK++RL+TLKAEITT+GGRV+NVLFVTG+++   +     
Sbjct: 260 IKASLCCEDRSDLLPDMIKTLKAMRLKTLKAEITTVGGRVKNVLFVTGEESSGEEVEEEY 319

Query: 317 SISSIQEALKAVMEKPGGGDDSSSGNIKRQR--TNNNINIL 324
            I +I+EALKAVMEK    + SSSGN KRQR  ++N I I+
Sbjct: 320 CIGTIEEALKAVMEKSNVEESSSSGNAKRQRMSSHNTITIV 359

BLAST of MS015308 vs. ExPASy Swiss-Prot
Match: Q9LS08 (Transcription factor AIG1 OS=Arabidopsis thaliana OX=3702 GN=BHLH32 PE=1 SV=1)

HSP 1 Score: 236.1 bits (601), Expect = 5.5e-61
Identity = 164/330 (49.70%), Postives = 201/330 (60.91%), Query Frame = 0

Query: 34  QHHLHHGVLGGGGRGLIFPAEVISPVLHPPWPPLPFKYEYSASSSSFGSLFNRRRTNCVQ 93
           Q HLHH          I P    S    P + PL F    +  S        R  ++   
Sbjct: 22  QFHLHH-------HPQILP---WSSTSLPSFDPLHFPSNPTRYSDPVHYFNRRASSSSSS 81

Query: 94  FGY-DG----PSAADHLG---RIISSTLGPV-GSGAPFGLQAE-LGKMSAQEIMDAKALA 153
           F Y DG    P + DH     RI+S  LGP+   G+ FG   E +GK+SAQE+MDAKALA
Sbjct: 82  FDYNDGFVSPPPSMDHPQNHLRILSEALGPIMRRGSSFGFDGEIMGKLSAQEVMDAKALA 141

Query: 154 ASKSHSEAERRRRERINNHLAKLRSLLPSTTKTDKASLLAEVIQHVKELKRQTCAIAETS 213
           ASKSHSEAERRRRERIN HLAKLRS+LP+TTKTDKASLLAEVIQH+KELKRQT  I +T 
Sbjct: 142 ASKSHSEAERRRRERINTHLAKLRSILPNTTKTDKASLLAEVIQHMKELKRQTSQITDTY 201

Query: 214 PIPSEADELTVDDASDESGGGKFVIKASLCCDDRSDLLPDLIKTLKSLRLRTLKAEITTL 273
            +P+E D+LTVD + ++   G  VI+AS CC DR+DL+ D+I  LKSLRLRTLKAEI T+
Sbjct: 202 QVPTECDDLTVDSSYNDE-EGNLVIRASFCCQDRTDLMHDVINALKSLRLRTLKAEIATV 261

Query: 274 GGRVRNVLFVT-----------------GD-----DNDSSSNNSISSIQEALKAVMEKPG 322
           GGRV+N+LF++                 GD     D +   NN +SSI+EALKAV+EK  
Sbjct: 262 GGRVKNILFLSREYDDEEDHDSYRRNFDGDDVEDYDEERMMNNRVSSIEEALKAVIEKCV 321

BLAST of MS015308 vs. ExPASy Swiss-Prot
Match: O80674 (Transcription factor bHLH106 OS=Arabidopsis thaliana OX=3702 GN=BHLH106 PE=2 SV=1)

HSP 1 Score: 154.1 bits (388), Expect = 2.8e-36
Identity = 96/205 (46.83%), Postives = 138/205 (67.32%), Query Frame = 0

Query: 128 LGKMSAQEIMDAKALAASKSHSEAERRRRERINNHLAKLRSLLPSTTKTDKASLLAEVIQ 187
           +G+  AQ+    +ALAA ++H EAERRRRERIN+HL KLR++L   +KTDKA+LLA+V+Q
Sbjct: 55  IGETMAQD----RALAALRNHKEAERRRRERINSHLNKLRNVLSCNSKTDKATLLAKVVQ 114

Query: 188 HVKELKRQTCAIAETSP--IPSEADELTVDDASDESGGGKFVIKASLCCDDRSDLLPDLI 247
            V+ELK+QT   +++    +PSE DE++V    D S  G  + KASLCC+DRSDLLPDL+
Sbjct: 115 RVRELKQQTLETSDSDQTLLPSETDEISVLHFGDYSNDGHIIFKASLCCEDRSDLLPDLM 174

Query: 248 KTLKSLRLRTLKAEITTLGGRVRNVLFVTGDDNDSSSNNSISSIQEALKAVMEKP----- 307
           + LKSL ++TL+AE+ T+GGR R+VL V   D +     S+  +Q ALK+++E+      
Sbjct: 175 EILKSLNMKTLRAEMVTIGGRTRSVLVVAA-DKEMHGVESVHFLQNALKSLLERSSKSLM 234

Query: 308 -----GGGDDSSSGNIKRQRTNNNI 321
                GGG + S    KR+R  ++I
Sbjct: 235 ERSSGGGGGERS----KRRRALDHI 250

BLAST of MS015308 vs. ExPASy Swiss-Prot
Match: Q9LET0 (Putative transcription factor bHLH107 OS=Arabidopsis thaliana OX=3702 GN=BHLH107 PE=3 SV=1)

HSP 1 Score: 145.6 bits (366), Expect = 9.8e-34
Identity = 89/193 (46.11%), Postives = 130/193 (67.36%), Query Frame = 0

Query: 136 IMDAKALAASKSHSEAERRRRERINNHLAKLRSLLPSTTKTDKASLLAEVIQHVKELKRQ 195
           + + KALA+ ++H EAER+RR RIN+HL KLR LL   +KTDK++LLA+V+Q VKELK+Q
Sbjct: 37  VYEDKALASLRNHKEAERKRRARINSHLNKLRKLLSCNSKTDKSTLLAKVVQRVKELKQQ 96

Query: 196 TCAIAETSPIPSEADELTVDDASDESGGG--KFVIKASLCCDDRSDLLPDLIKTLKSLRL 255
           T  I + + IPSE DE++V +  D S G   + + K S CC+DR +LL DL++TLKSL++
Sbjct: 97  TLEITDET-IPSETDEISVLNIEDCSRGDDRRIIFKVSFCCEDRPELLKDLMETLKSLQM 156

Query: 256 RTLKAEITTLGGRVRNVLFVTGDDNDSSSNNSISSIQEALKAVMEKP------GGGDDSS 315
            TL A++TT+GGR RNVL V   D +     S++ +Q ALK+++E+       G G    
Sbjct: 157 ETLFADMTTVGGRTRNVLVVAA-DKEHHGVQSVNFLQNALKSLLERSSKSVMVGHGGGGG 216

Query: 316 SGNIKRQRTNNNI 321
              +KR+R  ++I
Sbjct: 217 EERLKRRRALDHI 227

BLAST of MS015308 vs. ExPASy Swiss-Prot
Match: Q9XEF0 (Transcription factor bHLH51 OS=Arabidopsis thaliana OX=3702 GN=BHLH51 PE=2 SV=1)

HSP 1 Score: 114.0 bits (284), Expect = 3.2e-24
Identity = 70/158 (44.30%), Postives = 107/158 (67.72%), Query Frame = 0

Query: 140 KALAASKSHSEAERRRRERINNHLAKLRSLLPSTTKTDKASLLAEVIQHVKELKRQTCAI 199
           KA + S+SH  AE+RRR+RIN+HL  LR L+P++ K DKA+LLA VI+ VKELK++    
Sbjct: 59  KAESLSRSHRLAEKRRRDRINSHLTALRKLVPNSDKLDKAALLATVIEQVKELKQKAAES 118

Query: 200 AETSPIPSEADELTV--DDASD-ESGGGKFVIKASLCCDDRSDLLPDLIKTLKSLRLRTL 259
                +P+EADE+TV  +  SD ES     + KAS CC+D+ + + ++I+ L  L+L T+
Sbjct: 119 PIFQDLPTEADEVTVQPETISDFESNTNTIIFKASFCCEDQPEAISEIIRVLTKLQLETI 178

Query: 260 KAEITTLGGRVRNVLFVTGDDNDSSSNNSISSIQEALK 295
           +AEI ++GGR+R + F+  D N + + N I++  +ALK
Sbjct: 179 QAEIISVGGRMR-INFILKDSNCNETTN-IAASAKALK 214

BLAST of MS015308 vs. ExPASy TrEMBL
Match: A0A6J1DBM2 (transcription factor bHLH30-like OS=Momordica charantia OX=3673 GN=LOC111018619 PE=4 SV=1)

HSP 1 Score: 560.1 bits (1442), Expect = 6.2e-156
Identity = 298/323 (92.26%), Postives = 299/323 (92.57%), Query Frame = 0

Query: 1   CSQTIENMFQEQLLLQHQLQNNTNDNIIYGDHHQHHLHHGVLGGGGRGLIFPAEVISPVL 60
           CSQTIENMFQEQLLLQHQLQNNTNDNIIYGDHHQHHLHHGVLGGGGRGLIFPAEVISPVL
Sbjct: 19  CSQTIENMFQEQLLLQHQLQNNTNDNIIYGDHHQHHLHHGVLGGGGRGLIFPAEVISPVL 78

Query: 61  HPPWPPLPFKYEYSASSSSFGSLFNRRRTNCVQFGYDGPSAADHLGRIISSTLGPVGSGA 120
           HPPWPPLP                  R+ N   FGYDGPSAADHLGRIISSTLGPVGSGA
Sbjct: 79  HPPWPPLPV-----------------RQHNPFLFGYDGPSAADHLGRIISSTLGPVGSGA 138

Query: 121 PFGLQAELGKMSAQEIMDAKALAASKSHSEAERRRRERINNHLAKLRSLLPSTTKTDKAS 180
           PFGLQAELGKMSAQEIMDAKALAASKSHSEAERRRRERINNHLAKLRSLLPSTTKTDKAS
Sbjct: 139 PFGLQAELGKMSAQEIMDAKALAASKSHSEAERRRRERINNHLAKLRSLLPSTTKTDKAS 198

Query: 181 LLAEVIQHVKELKRQTCAIAETSPIPSEADELTVDDASDESGGGKFVIKASLCCDDRSDL 240
           LLAEVIQHVKELKRQTCAIAETSPIPSEADELTVDDASDESGGGKFVIKASLCCDDRSDL
Sbjct: 199 LLAEVIQHVKELKRQTCAIAETSPIPSEADELTVDDASDESGGGKFVIKASLCCDDRSDL 258

Query: 241 LPDLIKTLKSLRLRTLKAEITTLGGRVRNVLFVTGDDNDSSSNNSISSIQEALKAVMEKP 300
           LPDLIKTLKSLRLRTLKAEITTLGGRVRNVLFVT DD DSSSNNSISSIQEALKAVMEKP
Sbjct: 259 LPDLIKTLKSLRLRTLKAEITTLGGRVRNVLFVTRDDEDSSSNNSISSIQEALKAVMEKP 318

Query: 301 GGGDDSSSGNIKRQRTNNNINIL 324
           GGGDDSSSGNIKRQRTNNNINIL
Sbjct: 319 GGGDDSSSGNIKRQRTNNNINIL 324

BLAST of MS015308 vs. ExPASy TrEMBL
Match: A0A6J1G0W5 (transcription factor bHLH30-like OS=Cucurbita moschata OX=3662 GN=LOC111449644 PE=4 SV=1)

HSP 1 Score: 456.1 bits (1172), Expect = 1.3e-124
Identity = 270/361 (74.79%), Postives = 286/361 (79.22%), Query Frame = 0

Query: 1   CSQTIENMFQEQLLLQHQLQNNTNDNIIYGDHHQHHLHHGVLGGGGRGLIFPAEVISPVL 60
           CSQTIENMFQEQLLL  QLQNNT D+IIYGDHH H        G GRGLIFPA+V+SPVL
Sbjct: 18  CSQTIENMFQEQLLLHQQLQNNTTDHIIYGDHHHH--------GAGRGLIFPADVVSPVL 77

Query: 61  HPPWPPL----------PFKYEYSASSSSFGSLFNRRRTNCVQFGYDGPSAADHLGRIIS 120
           +  WPPL          P       SSSS+G+LFNRR  NC+QF YDG S+ADHLGRIIS
Sbjct: 78  N-SWPPLNPFLIPPPPPPPPPTSLCSSSSYGNLFNRRPANCLQFAYDGTSSADHLGRIIS 137

Query: 121 STLGPV---GSGAPFGLQAELGKMSAQEIMDAKALAASKSHSEAERRRRERINNHLAKLR 180
           +TLGPV   GS APFGLQAELGKMSAQEIMDAKALAASKSHSEAERRRRERINNHLAKLR
Sbjct: 138 TTLGPVVHHGSTAPFGLQAELGKMSAQEIMDAKALAASKSHSEAERRRRERINNHLAKLR 197

Query: 181 SLLPSTTKTDKASLLAEVIQHVKELKRQTCAIAETSPIPSEADELTVDDASDES--GGGK 240
           SLLPSTTKTDKASLLAEVIQHVKELKRQT  IAETSPIP++ DELTVDDASDE    G K
Sbjct: 198 SLLPSTTKTDKASLLAEVIQHVKELKRQTSLIAETSPIPTDIDELTVDDASDEMMINGAK 257

Query: 241 FVIKASLCCDDRSDLLPDLIKTLKSLRLRTLKAEITTLGGRVRNVLFVTGDD-------- 300
           FVIKASLCC+DRSDLLPDLIKTLKSLRLRTLKAEITTLGGRVRNVLFVTG+D        
Sbjct: 258 FVIKASLCCEDRSDLLPDLIKTLKSLRLRTLKAEITTLGGRVRNVLFVTGEDDEPSTDHN 317

Query: 301 -NDSSSNN--------------SISSIQEALKAVMEKPGGGDDSSSGNIKRQRTNNNINI 324
            ND+++NN              SISSIQEALKAVMEK  G DDSSSGNIKRQRT NNINI
Sbjct: 318 NNDNNNNNNNPNSDSQQQQQQHSISSIQEALKAVMEKT-GPDDSSSGNIKRQRT-NNINI 367

BLAST of MS015308 vs. ExPASy TrEMBL
Match: A0A6J1HUT9 (transcription factor bHLH30-like OS=Cucurbita maxima OX=3661 GN=LOC111467674 PE=4 SV=1)

HSP 1 Score: 447.2 bits (1149), Expect = 5.8e-122
Identity = 267/359 (74.37%), Postives = 284/359 (79.11%), Query Frame = 0

Query: 1   CSQTIENMFQEQLLLQHQLQNNTNDNIIYGDHHQHHLHHGVLGGGGRGLIFPAEVISPVL 60
           CSQTIENMFQEQLLL HQLQNN  D+IIYGDHH H        G GRGLIFP +V+SPVL
Sbjct: 17  CSQTIENMFQEQLLL-HQLQNNNTDHIIYGDHHHH--------GAGRGLIFPGDVVSPVL 76

Query: 61  HPPWPPL----------PFKYEYSASSSSFGSLFNRRRTNCVQFGYDGPSAADHLGRIIS 120
           +  WPPL          P       SSSS+G+LFNRR  NC+QF YDG S+ADHLGRIIS
Sbjct: 77  N-SWPPLNPFLIPPPPPPPPPTSLCSSSSYGNLFNRRPANCLQFAYDGTSSADHLGRIIS 136

Query: 121 STLGPV---GSGAPFGLQAELGKMSAQEIMDAKALAASKSHSEAERRRRERINNHLAKLR 180
           +TLGPV   GS APFGLQAELGKMSAQEIMDAKALAASKSHSEAERRRRERINNHLAKLR
Sbjct: 137 TTLGPVVHHGSTAPFGLQAELGKMSAQEIMDAKALAASKSHSEAERRRRERINNHLAKLR 196

Query: 181 SLLPSTTKTDKASLLAEVIQHVKELKRQTCAIAETSPIPSEADELTVDDASDES--GGGK 240
           SLLPSTTKTDKASLLAEVIQHVKELKRQT  IAETS IP++ DELTVDDASDE    G K
Sbjct: 197 SLLPSTTKTDKASLLAEVIQHVKELKRQTSLIAETSSIPTDIDELTVDDASDEMMINGAK 256

Query: 241 FVIKASLCCDDRSDLLPDLIKTLKSLRLRTLKAEITTLGGRVRNVLFVTGDD-------- 300
           FVIKASLCC+DRSDLLPDLIKTLKSLRLRTLKAEITTLGGRVRNVLFVTG+D        
Sbjct: 257 FVIKASLCCEDRSDLLPDLIKTLKSLRLRTLKAEITTLGGRVRNVLFVTGEDDEPSTDHN 316

Query: 301 ---NDSSSNN----------SISSIQEALKAVMEKPGGGDDSSSGNIKRQRTNNNINIL 324
              N++++NN          SISSIQEALKAVMEK  G DDSSSGNIKRQRT NNINIL
Sbjct: 317 NNNNNNNNNNPNSDSQQQQHSISSIQEALKAVMEKT-GPDDSSSGNIKRQRT-NNINIL 363

BLAST of MS015308 vs. ExPASy TrEMBL
Match: A0A6J1GZ49 (transcription factor bHLH30-like OS=Cucurbita moschata OX=3662 GN=LOC111458472 PE=4 SV=1)

HSP 1 Score: 426.8 bits (1096), Expect = 8.1e-116
Identity = 248/341 (72.73%), Postives = 270/341 (79.18%), Query Frame = 0

Query: 1   CSQTIENMFQEQLLLQHQLQNNTNDNIIYGDHHQHHLHHGVLGGGGRGLIFPAEVISPVL 60
           CSQTIENMFQ+QLLL   LQNN +D+   GDHH          G GRGLIFP E++SPVL
Sbjct: 17  CSQTIENMFQQQLLLHQHLQNNDDDDNNNGDHHH---------GTGRGLIFPPELVSPVL 76

Query: 61  H----------PPWPPLPFKYEYSASSSSFGSLFNRRRTNCVQFGYDGPSAADHLGRIIS 120
                      PP P LP      +SSSS+G LFNRR  NCVQF YDG S+ADHLG+IIS
Sbjct: 77  QPWSSLNPFMIPPPPSLP-----CSSSSSYGGLFNRRPPNCVQFAYDGSSSADHLGQIIS 136

Query: 121 STLGPV---GSGAPFGLQAELGKMSAQEIMDAKALAASKSHSEAERRRRERINNHLAKLR 180
           +TLGPV   GS APFGLQAELGKMSAQEIMDAKALAASKSHSEAERRRRERINNHLAKLR
Sbjct: 137 TTLGPVVHPGSMAPFGLQAELGKMSAQEIMDAKALAASKSHSEAERRRRERINNHLAKLR 196

Query: 181 SLLPSTTKTDKASLLAEVIQHVKELKRQTCAIAETSPIPSEADELTVDDASDES--GGGK 240
           SLLP+TTKTDKASLLAEVIQHVKELKRQT  IAETSPIP+E DE+TVDDAS+E    G K
Sbjct: 197 SLLPNTTKTDKASLLAEVIQHVKELKRQTSLIAETSPIPTEVDEVTVDDASEEMMISGAK 256

Query: 241 FVIKASLCCDDRSDLLPDLIKTLKSLRLRTLKAEITTLGGRVRNVLFVTGDDNDS---SS 300
           FVIKASLCC+DRSDLLPDLIK LKSLRLRTLKAEITTLGGRVRNVLF+T ++        
Sbjct: 257 FVIKASLCCEDRSDLLPDLIKALKSLRLRTLKAEITTLGGRVRNVLFITAEEEQQHGPEQ 316

Query: 301 NNSISSIQEALKAVMEKPGGGDDSSSGNIKRQRTNNNINIL 324
            +++SSIQEALKAVME+  GGDDSSS NIKR RT NNINIL
Sbjct: 317 QHNMSSIQEALKAVMERT-GGDDSSSANIKRLRTTNNINIL 342

BLAST of MS015308 vs. ExPASy TrEMBL
Match: A0A1S3BFZ7 (transcription factor bHLH30 OS=Cucumis melo OX=3656 GN=LOC103489248 PE=4 SV=1)

HSP 1 Score: 423.7 bits (1088), Expect = 6.9e-115
Identity = 249/358 (69.55%), Postives = 269/358 (75.14%), Query Frame = 0

Query: 1   CSQTIENMFQEQLLLQHQLQNNTNDNIIYGDHHQ----HHLHHGVLGGGGRGLIFPAEVI 60
           CSQTIENMFQEQLLLQ QLQNN  D     DHH      H HHG+  G    LIFP EV+
Sbjct: 18  CSQTIENMFQEQLLLQQQLQNNDGDQ-SNNDHHMIYGVEHHHHGI--GRSGSLIFPPEVM 77

Query: 61  SPVLH----------PPWPPLPFKYEYSASSSSFGSLFNRRRTNCVQFGYDGPSAADHLG 120
            P+L           PP PP P     S SSSS+GSLFNRR  NC+QF YDGPS+ADHLG
Sbjct: 78  PPMLQPWSSLNPFMIPPPPPPPLPTSLSCSSSSYGSLFNRRPPNCLQFAYDGPSSADHLG 137

Query: 121 RIISSTLGPV---GSGAPFGLQAELGKMSAQEIMDAKALAASKSHSEAERRRRERINNHL 180
           RIIS+TLGPV   GS APFGLQAELGKMSAQEIMDAKALAASKSHSEAERRRRERINNHL
Sbjct: 138 RIISTTLGPVVHPGSTAPFGLQAELGKMSAQEIMDAKALAASKSHSEAERRRRERINNHL 197

Query: 181 AKLRSLLPSTTKTDKASLLAEVIQHVKELKRQTCAIAETSPIPSEADELTVDDASDE--- 240
           AKLRS+LPSTTKTDKASLLAEVI+HVKELKRQT  IAETSPIP+E DE++VDDAS++   
Sbjct: 198 AKLRSILPSTTKTDKASLLAEVIEHVKELKRQTSIIAETSPIPTEVDEVSVDDASEQEMM 257

Query: 241 --------SGGGKFVIKASLCCDDRSDLLPDLIKTLKSLRLRTLKAEITTLGGRVRNVLF 300
                   S   KFVIKASLCC+DRSDLLPDLIKTLKSLRL TLKAEITTLGGR+RNVLF
Sbjct: 258 MISNNGSISSSAKFVIKASLCCEDRSDLLPDLIKTLKSLRLTTLKAEITTLGGRLRNVLF 317

Query: 301 VTGDDNDSSSNNSISSIQEALKAVMEKPGGGDDSSSGNIKRQRT-------NNNINIL 324
           VT D+     +N  S IQ+ALKAV+EK  G  DSSS NIKRQRT       NNN NIL
Sbjct: 318 VTADEEQQQQHNITSIIQDALKAVIEKTAGDHDSSSANIKRQRTTTTNNINNNNNNIL 372

BLAST of MS015308 vs. TAIR 10
Match: AT1G68810.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 309.7 bits (792), Expect = 2.8e-84
Identity = 201/341 (58.94%), Postives = 243/341 (71.26%), Query Frame = 0

Query: 17  HQLQNNTNDNIIYG--DHHQHHLHH-------GVLGGGGRGL-IFPAEVISPV--LHPP- 76
           + +QN  ND   +    HH HH H        G  G  G G  IF  + +SP+  L PP 
Sbjct: 20  NNIQNYQNDLFFHQLISHHHHHHHDPSQSETLGASGNVGSGFTIFSQDSVSPIWSLPPPT 79

Query: 77  --WPPL-PFKYEYSASSSSFGSLFNRRRTN--CVQFGYDG----PSAADHLG---RIISS 136
              PP   F    S+ +S +GS FNR R +   +QFGY+G     SAA H     RI+S 
Sbjct: 80  SIQPPFDQFPPPSSSPASFYGSFFNRSRAHHQGLQFGYEGFGGATSAAHHHHEQLRILSE 139

Query: 137 TLGPV---GSGAPFGLQAELGKMSAQEIMDAKALAASKSHSEAERRRRERINNHLAKLRS 196
            LGPV   GSG PFGLQAELGKM+AQEIMDAKALAASKSHSEAERRRRERINNHLAKLRS
Sbjct: 140 ALGPVVQAGSG-PFGLQAELGKMTAQEIMDAKALAASKSHSEAERRRRERINNHLAKLRS 199

Query: 197 LLPSTTKTDKASLLAEVIQHVKELKRQTCAIAETSPIPSEADELTVD-DASDESGGGKFV 256
           +LP+TTKTDKASLLAEVIQHVKELKR+T  I+ET+ +P+E+DELTV     +E+G G+FV
Sbjct: 200 ILPNTTKTDKASLLAEVIQHVKELKRETSVISETNLVPTESDELTVAFTEEEETGDGRFV 259

Query: 257 IKASLCCDDRSDLLPDLIKTLKSLRLRTLKAEITTLGGRVRNVLFVTGDDN---DSSSNN 316
           IKASLCC+DRSDLLPD+IKTLK++RL+TLKAEITT+GGRV+NVLFVTG+++   +     
Sbjct: 260 IKASLCCEDRSDLLPDMIKTLKAMRLKTLKAEITTVGGRVKNVLFVTGEESSGEEVEEEY 319

Query: 317 SISSIQEALKAVMEKPGGGDDSSSGNIKRQR--TNNNINIL 324
            I +I+EALKAVMEK    + SSSGN KRQR  ++N I I+
Sbjct: 320 CIGTIEEALKAVMEKSNVEESSSSGNAKRQRMSSHNTITIV 359

BLAST of MS015308 vs. TAIR 10
Match: AT3G25710.1 (basic helix-loop-helix 32 )

HSP 1 Score: 236.1 bits (601), Expect = 3.9e-62
Identity = 164/330 (49.70%), Postives = 201/330 (60.91%), Query Frame = 0

Query: 34  QHHLHHGVLGGGGRGLIFPAEVISPVLHPPWPPLPFKYEYSASSSSFGSLFNRRRTNCVQ 93
           Q HLHH          I P    S    P + PL F    +  S        R  ++   
Sbjct: 22  QFHLHH-------HPQILP---WSSTSLPSFDPLHFPSNPTRYSDPVHYFNRRASSSSSS 81

Query: 94  FGY-DG----PSAADHLG---RIISSTLGPV-GSGAPFGLQAE-LGKMSAQEIMDAKALA 153
           F Y DG    P + DH     RI+S  LGP+   G+ FG   E +GK+SAQE+MDAKALA
Sbjct: 82  FDYNDGFVSPPPSMDHPQNHLRILSEALGPIMRRGSSFGFDGEIMGKLSAQEVMDAKALA 141

Query: 154 ASKSHSEAERRRRERINNHLAKLRSLLPSTTKTDKASLLAEVIQHVKELKRQTCAIAETS 213
           ASKSHSEAERRRRERIN HLAKLRS+LP+TTKTDKASLLAEVIQH+KELKRQT  I +T 
Sbjct: 142 ASKSHSEAERRRRERINTHLAKLRSILPNTTKTDKASLLAEVIQHMKELKRQTSQITDTY 201

Query: 214 PIPSEADELTVDDASDESGGGKFVIKASLCCDDRSDLLPDLIKTLKSLRLRTLKAEITTL 273
            +P+E D+LTVD + ++   G  VI+AS CC DR+DL+ D+I  LKSLRLRTLKAEI T+
Sbjct: 202 QVPTECDDLTVDSSYNDE-EGNLVIRASFCCQDRTDLMHDVINALKSLRLRTLKAEIATV 261

Query: 274 GGRVRNVLFVT-----------------GD-----DNDSSSNNSISSIQEALKAVMEKPG 322
           GGRV+N+LF++                 GD     D +   NN +SSI+EALKAV+EK  
Sbjct: 262 GGRVKNILFLSREYDDEEDHDSYRRNFDGDDVEDYDEERMMNNRVSSIEEALKAVIEKCV 321

BLAST of MS015308 vs. TAIR 10
Match: AT2G41130.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 154.1 bits (388), Expect = 2.0e-37
Identity = 96/205 (46.83%), Postives = 138/205 (67.32%), Query Frame = 0

Query: 128 LGKMSAQEIMDAKALAASKSHSEAERRRRERINNHLAKLRSLLPSTTKTDKASLLAEVIQ 187
           +G+  AQ+    +ALAA ++H EAERRRRERIN+HL KLR++L   +KTDKA+LLA+V+Q
Sbjct: 55  IGETMAQD----RALAALRNHKEAERRRRERINSHLNKLRNVLSCNSKTDKATLLAKVVQ 114

Query: 188 HVKELKRQTCAIAETSP--IPSEADELTVDDASDESGGGKFVIKASLCCDDRSDLLPDLI 247
            V+ELK+QT   +++    +PSE DE++V    D S  G  + KASLCC+DRSDLLPDL+
Sbjct: 115 RVRELKQQTLETSDSDQTLLPSETDEISVLHFGDYSNDGHIIFKASLCCEDRSDLLPDLM 174

Query: 248 KTLKSLRLRTLKAEITTLGGRVRNVLFVTGDDNDSSSNNSISSIQEALKAVMEKP----- 307
           + LKSL ++TL+AE+ T+GGR R+VL V   D +     S+  +Q ALK+++E+      
Sbjct: 175 EILKSLNMKTLRAEMVTIGGRTRSVLVVAA-DKEMHGVESVHFLQNALKSLLERSSKSLM 234

Query: 308 -----GGGDDSSSGNIKRQRTNNNI 321
                GGG + S    KR+R  ++I
Sbjct: 235 ERSSGGGGGERS----KRRRALDHI 250

BLAST of MS015308 vs. TAIR 10
Match: AT3G56770.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 145.6 bits (366), Expect = 7.0e-35
Identity = 89/193 (46.11%), Postives = 130/193 (67.36%), Query Frame = 0

Query: 136 IMDAKALAASKSHSEAERRRRERINNHLAKLRSLLPSTTKTDKASLLAEVIQHVKELKRQ 195
           + + KALA+ ++H EAER+RR RIN+HL KLR LL   +KTDK++LLA+V+Q VKELK+Q
Sbjct: 37  VYEDKALASLRNHKEAERKRRARINSHLNKLRKLLSCNSKTDKSTLLAKVVQRVKELKQQ 96

Query: 196 TCAIAETSPIPSEADELTVDDASDESGGG--KFVIKASLCCDDRSDLLPDLIKTLKSLRL 255
           T  I + + IPSE DE++V +  D S G   + + K S CC+DR +LL DL++TLKSL++
Sbjct: 97  TLEITDET-IPSETDEISVLNIEDCSRGDDRRIIFKVSFCCEDRPELLKDLMETLKSLQM 156

Query: 256 RTLKAEITTLGGRVRNVLFVTGDDNDSSSNNSISSIQEALKAVMEKP------GGGDDSS 315
            TL A++TT+GGR RNVL V   D +     S++ +Q ALK+++E+       G G    
Sbjct: 157 ETLFADMTTVGGRTRNVLVVAA-DKEHHGVQSVNFLQNALKSLLERSSKSVMVGHGGGGG 216

Query: 316 SGNIKRQRTNNNI 321
              +KR+R  ++I
Sbjct: 217 EERLKRRRALDHI 227

BLAST of MS015308 vs. TAIR 10
Match: AT2G40200.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 114.0 bits (284), Expect = 2.2e-25
Identity = 70/158 (44.30%), Postives = 107/158 (67.72%), Query Frame = 0

Query: 140 KALAASKSHSEAERRRRERINNHLAKLRSLLPSTTKTDKASLLAEVIQHVKELKRQTCAI 199
           KA + S+SH  AE+RRR+RIN+HL  LR L+P++ K DKA+LLA VI+ VKELK++    
Sbjct: 59  KAESLSRSHRLAEKRRRDRINSHLTALRKLVPNSDKLDKAALLATVIEQVKELKQKAAES 118

Query: 200 AETSPIPSEADELTV--DDASD-ESGGGKFVIKASLCCDDRSDLLPDLIKTLKSLRLRTL 259
                +P+EADE+TV  +  SD ES     + KAS CC+D+ + + ++I+ L  L+L T+
Sbjct: 119 PIFQDLPTEADEVTVQPETISDFESNTNTIIFKASFCCEDQPEAISEIIRVLTKLQLETI 178

Query: 260 KAEITTLGGRVRNVLFVTGDDNDSSSNNSISSIQEALK 295
           +AEI ++GGR+R + F+  D N + + N I++  +ALK
Sbjct: 179 QAEIISVGGRMR-INFILKDSNCNETTN-IAASAKALK 214

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022150481.11.3e-15592.26transcription factor bHLH30-like [Momordica charantia][more]
XP_022945399.12.6e-12474.79transcription factor bHLH30-like [Cucurbita moschata][more]
KAG7013054.17.5e-12474.72Transcription factor bHLH30, partial [Cucurbita argyrosperma subsp. argyrosperma... [more]
KAG6573995.19.8e-12474.52Transcription factor basic helix-loop-helix 30, partial [Cucurbita argyrosperma ... [more]
XP_023542825.11.7e-12374.44transcription factor bHLH30-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q9S7Y13.9e-8358.94Transcription factor bHLH30 OS=Arabidopsis thaliana OX=3702 GN=BHLH30 PE=1 SV=1[more]
Q9LS085.5e-6149.70Transcription factor AIG1 OS=Arabidopsis thaliana OX=3702 GN=BHLH32 PE=1 SV=1[more]
O806742.8e-3646.83Transcription factor bHLH106 OS=Arabidopsis thaliana OX=3702 GN=BHLH106 PE=2 SV=... [more]
Q9LET09.8e-3446.11Putative transcription factor bHLH107 OS=Arabidopsis thaliana OX=3702 GN=BHLH107... [more]
Q9XEF03.2e-2444.30Transcription factor bHLH51 OS=Arabidopsis thaliana OX=3702 GN=BHLH51 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1DBM26.2e-15692.26transcription factor bHLH30-like OS=Momordica charantia OX=3673 GN=LOC111018619 ... [more]
A0A6J1G0W51.3e-12474.79transcription factor bHLH30-like OS=Cucurbita moschata OX=3662 GN=LOC111449644 P... [more]
A0A6J1HUT95.8e-12274.37transcription factor bHLH30-like OS=Cucurbita maxima OX=3661 GN=LOC111467674 PE=... [more]
A0A6J1GZ498.1e-11672.73transcription factor bHLH30-like OS=Cucurbita moschata OX=3662 GN=LOC111458472 P... [more]
A0A1S3BFZ76.9e-11569.55transcription factor bHLH30 OS=Cucumis melo OX=3656 GN=LOC103489248 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT1G68810.12.8e-8458.94basic helix-loop-helix (bHLH) DNA-binding superfamily protein [more]
AT3G25710.13.9e-6249.70basic helix-loop-helix 32 [more]
AT2G41130.12.0e-3746.83basic helix-loop-helix (bHLH) DNA-binding superfamily protein [more]
AT3G56770.17.0e-3546.11basic helix-loop-helix (bHLH) DNA-binding superfamily protein [more]
AT2G40200.12.2e-2544.30basic helix-loop-helix (bHLH) DNA-binding superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR011598Myc-type, basic helix-loop-helix (bHLH) domainSMARTSM00353finuluscoord: 149..198
e-value: 2.1E-13
score: 60.6
IPR011598Myc-type, basic helix-loop-helix (bHLH) domainPFAMPF00010HLHcoord: 146..192
e-value: 1.5E-12
score: 47.3
IPR011598Myc-type, basic helix-loop-helix (bHLH) domainPROSITEPS50888BHLHcoord: 143..192
score: 16.907291
IPR036638Helix-loop-helix DNA-binding domain superfamilyGENE3D4.10.280.10coord: 138..198
e-value: 2.1E-16
score: 61.5
IPR036638Helix-loop-helix DNA-binding domain superfamilySUPERFAMILY47459HLH, helix-loop-helix DNA-binding domaincoord: 147..196
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 298..323
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 307..323
NoneNo IPR availablePANTHERPTHR45844TRANSCRIPTION FACTOR BHLH30coord: 56..319
NoneNo IPR availablePANTHERPTHR45844:SF2TRANSCRIPTION FACTOR BHLH30coord: 56..319
NoneNo IPR availableCDDcd11455bHLH_AtAIG1_likecoord: 138..215
e-value: 8.81037E-32
score: 112.002
NoneNo IPR availableSUPERFAMILY55021ACT-likecoord: 231..299

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS015308.1MS015308.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0046983 protein dimerization activity