MS015239 (gene) Bitter gourd (TR) v1

Overview
NameMS015239
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
Descriptionasparagine synthetase domain-containing protein 1 isoform X2
Locationscaffold2: 1977880 .. 1984265 (+)
RNA-Seq ExpressionMS015239
SyntenyMS015239
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTGTGGAATCGCTTTGATTTTTTCTGGCGTTCGCCTCAATTTGTCACTTCTGCATGTAGACGCCAGATCGCCGTCTCTTCACCCAGTCCACGAGCAAGTGAGTCATCTTTTAGCTTTTAAATTCTACGACTCCTCAATTTCTTCCCATTCAGTTATACAGTTCGTTATAATTTGGATTAATTTCTGTGGCGCGAATTCGATTTTGGGTTTGTATGATGTTCTTTTTGGCTATGTATTGATTTTAGTTTCGTTTTCCTGCTTCAGCTCGAGTTTCCTATAGATGGTCTTAAGGCGGCGTTGCGGAGAAGGGGACCCGATAGCTTAGGCGGAGTGAAGGTTTTTATCTCGTCGAAATCTTCTAGTCATGGCGAAGAAAATCTGTTTGAGTGCTTCATTGAGAGGGTTGATGATGGAGATGCATTCGAGAATCAAAATGATCGATACAAGATGGAAAATAGTTGCTTCTGTTCGGAAAATGGATTGGTTCTTCAATCAGAGAACAGTCATAGCGATCCCACTTCTGTTGCGGAGTTGTATTTAATAGGGGCTACGTTGCAGCTTAGAGGGCTTAACCCTATTGTTCAGCCACTGATGGATGCTTCCCGCAATATTCTTGTTTACAATGGTAAGCTTTATGCAGAAACTTATTTACTCAAGTTCGACATTTGGCTTTTTTTAATTTGTTCATTGATTATACCTTTTCCGTTGCGTATGACATTGATCATTTATGACGCCATATGAATATGCTGCGTCTTATATATCTGGTCCGGCAGAACCTTATCACCAGTTAGGTCCTTTTTTTTGGGTCCATCTTATTTTTAACTATTATAGCAGGAACTATAATAAATTTATTTCATATCGTTTTCATTTTCAAAAGGCGAAATATTTGGAGGAATACCCATTGAGCGTGATGAAAATGATGCTGAAACACTTATGCAAGCTCTAGGGACTTGTTGCGCCAGCAATTTTAATTTAGATATAAGCACGTGTAACGGTGAAGGTAAGGGACAAAGCACAATCGTAGATATTCTTTCAAAGATTCAAGGGCCATGGTCAATCATCTACTGGCAGGTAACTGTTAGTGGTAAGTTTCTCTGTTATGTTAACTGGCCCTGTGCACCGTCTATCCTTGTGTTTTCTTGTCGTTCACTTTTAACCAAACCTATCATGTTTAGGATAGTGCAAAAACTTTGTGGTTTGGTCGCGATGCCTTTGGTAGGCGAAGTCTTCTTGTTCACTGGCCAACAGTGGAGGATTCTCGGTTTCTGTTGTCTTCTGCATCACCAGCTTCTTCCATATTTCACAGCTCTGGTATACTGTGCATGGTAGCACTTTACGTATCTTCTGTTGGTGTTATGTAGTCAGTTTATTGCTTATTAATGATTTCTTTTTGTGCTTGTGGTATTATACTATCTGCAGGGCTTGATGATGATAATGGTACTTGTGAAGTGAGCTTCTGGGAAGAACTTCCATGTGGGATTTATAGCATCTCTTTTGATGCTCAGAGCAGGGACTGTTGCCTGGTCGGTGCAGTCAAAAAGCATGAGTGGACCAATGAGTCACTGAAAGAACTTGTTGAGTGGAAGAGAACTCGTATTGAACCTGAACCTGGAAATATCTCCGCCTCATATCTCAAGGATTTTTTGTCACAGCATGAATGCAACTCTGGATGTTTACATAACTTGCAATCCATATCAGGTATCTATGATTCCTTTTCATTGTGGTACCAAAACTTATAAACCAGAGTTCCAATTCTTATGGTAGTTTGTTTGCTGTCACTACCACCTTGTCAATGAAAAAAATATCTGGTTTTGATGAAGAATGTAATTTCTCGACATCTTTGATGTTGCTTTTGTTGATTGAAAAGGGAGTATTGAAGCTCCTGTTTCAACACCTGCGGAGATTGTGTTGGATGCTCTAAGAAAGTCTCTGATCCGGCGTACTTCACTTTACACAGTTTTCCAGGTTCAAAATTCCACTCTGTGGCTCTTCTATTGGTTTGTATTAAGATAGTACTTCTGAGCATGTGTTTCCTTTGAGCTGTTTTGTTTTACCGTGAAAATCTCTCTCTTGGTTTGCTTAAATTTTTGCACAGTGCACAAGCATAGTTTTGCAGCATGTATTTTTGCTGATTAAGCACTTGCGATTTAAAACTTTGTCATTGAAGACTTAGGTAATAATAAATTTAGTTACCTTTGGCATTTTTTTATTTTTCTCTTCTATTTAGTTGTGAAATCAGCACAGGTTAGAGGCTACCCGTGTATTCCATAGTTCTCTGGACAAGCTCTTTTCTAATAGAGTTTATAATATTTACTGGCATGTCTTAATGGTGGCAAACACTCATAGAAGAGACAAGCACACTGCTAAAGCCTAAATTACAGTAAATTCTTTTGTAGTTACATTCTTTCCATGTTTTTGAACTACTGGAAAGCTTTTATATAGCTAGTTTGGGTGGGGTTCTCTCTACCCCCGCCCTTAGTTTGTTTTCTTTCCTCTTTTGCTTAATAACAGGTTTCTTATCCCCCCCCCAAAAAAAAGAAAAAAAGTCTGAAAGGGAGAAATTTATTGTGAATGCATAGGGCACAACATCTGCTTCCAGAAAAGCCGAGCTTGGTCCAGTGGCCATTCTTTTTTCTGGTGGATTGGATTCTATGATACTAGCAGCATTACTGGATGAGTGCCTAGATGCCTGTTGTAAGTGTTTATTAGTTCACCTGAATGTCAATCATATTCTATTATTAAACTTGATGTGTGGGGGCCGAGATACTGAATGAAGTGATGTTACTTTAATGCCTAGTCCCAGTTAGTTGATTAATTGGTCTCATCGTCTCTACAGATGAAATTGATCTTCTTAATGTCAGCTTTGATGGTTGTTCTGCACCTGATCGAATCTCTGCAAAGGCTGGAGTTCAGGAACTAAGAAGGATTGCACCTCTAAGAAGGCATTATTACTGAGCTTTATCTTGGGTTTTGCATTGTCCTTATTGTGGGTCAATTTTTTTAGTGCTAAATATGTGGAGTTTTTTTAGTTTACTAAAACCACAGAGTAAAAATATGAAGTAGGAAACAAAAATGAAAATAAAGTGTTATGCTTGAAAGTTTTTGCTAATTTGAGTCCGGTGATATCATATAGTTGGAAACAAAACGAAAATAAAATGCATGATACTGTTTTTCAGGGTAGGTCTGTTTTATCATCTTTCGGATAAGATAGTAGAATCTGGGTTATTATTTCATTGGCATCTTGTTTGGTTACTGGGCTTGCAATATCTTATTGGGCTTGCAATATCTTATTAGTAGTCCCTTGGAATCAAAGTTAACTCCAACTAAAATAATTTGGAAAACAAATGGGTCTAAGAATAAATAAAATTTTATCTGTAGTTAGTGATTCTCTAGAAGTTAAGACCGGTGACTTCCTTCAATCAAAATAATATATTATGTCCTAGCACTTAAGAGATATGATTTGCAGTAGGGGAAAAAAAAATTGAAAGTTTCATGGCCCTTCACTCTGTCTTGTGCTTGAAACAGCGATCTTGCTAGTGCTTACATTTTCTTTCTTTTCCTCTTATGTGGATTGTGGAATGTTTTATTTTATTTTCTACACCCTCACTTTATTTAAAATTTTAGGTGGAAGCTGGTCGAGGTTGATGCTGATTTGTCAGATTTGACCTCTGAAACCAAGCATGTCATATCTCTAATAAATCCTGCAAATACGTACATGGTACCCTACTTTTGTTGAGTAAATAATATAGTTTAGTGTTTGCCAACTTGGTTTTATGTTAGCATTCTGATAGACCTGCCTTGATGCGAGTTATTATATTGCATGGAATCACCAATCTATTCCTATGCTCCAAGTAAAATGAGATTATGGCCCAATTCAGTGTTTATTGGGTTAAAAAAACGAAATTATGGTTAAACCCTTCACATTTGCTGTGTGATATGTATATCACAAATATATGATTTTAAGGGTTTTGAACTTGTGGCCTCTTTGGTTGAGAGTCCATGGACTGTTAACTCTGTTTAACTTTTGGGTTGTTCTCATAATCCTTGTTGTAATGACTAATGCTTATTGGGTTTGAACTTGAGGTTTTCACTCTGCCTGCAATATTTAGTAATTTGGAAAAAAGTCGGGAGATATTTGGTATTCCATATTAGCAGTGGGAGGGTTTAGATATCCAAGGTCTTATCAGTTATCACCATTGGGGATGACGTTCGTATGTGTAAAACAGTAATCCTTTCTACCATGTGTTTTTTTGGCTATTTTTTCTGCAAGTATTCTGGTCAAGATGAATTCATATCTCATTATAAACCCCACCCTTAAAATTTGTGTGCCTGACTTCAATGCAACAAATTTTAAGCTTGGTAGACTGAGCGGCTAGTATCATCCCATTGATAGTTCTTCGTTACCTTTTATTTAATTGCAGGACCTGAATATTGGAATTGCTTTGTGGCTGGCCTCTGGTGGCAATGGTTGGATTTACGAAGCAATTGACAAGGATGAGAAGTTTCATCGACGTGTTAAATACAAGTCTGAGGCCAAAATTCTCTTGGTTGGTTCTGGTGCTGATGAGCAATGTGCTGGCTATGGTAGGCATCGAACAAAATTTAGACAAGGGGGGTAAGTGTAAACTGTTGTCATTTCATACATAAGCTTTGCGTTTCTATAATTTCTAGAACATACTGCATTGTCACATTCTGATGTTCATTGCATAACCTTTATTATTTTTTATCATCATTTGATCTGTTGGTATAGAAATGTGAAGATTCGAACTCATCTATGGGGATAGAAATGACATCAGAAGAACTTGTATATTAGGGAGGGTGTCTTTTCAACATAGAAGATGTTCCTTTTCCTTTAAGCATGCTCATGCACAAGCATAGGACTTGGATATTTGTTAAAGCTCATTTTTGGTAAAATGTGAGACCATAAAAATGTTTTTGCAGTGTATAATTTAGTGTCCTGCAATGTTCCCTTCAGCTGGCATCTTTTAAACGAGGAAATGAAATTGGATATTCAGAGAATCTGGAAGAGAAATCTAGGGAGAGATGATAGATGTATTGCTGATAATGGCAAGGAGGTATTTCCTAGAAATGATGTGCCAATGCTACTGTTGCATGTCTCATTTTTTCCTCATTTAACTAAAATTTCGCTCCTTTTCTCAGGCTAGATTTCCATTTTTGGATGAAGACGTTATAAATACATTGCTATCATTTCCTCTATGGGAGGTCACTGACCTTGAGAAACCTGTCGGAACCGGTGATAAGAAGATACTTAGGGAGGTCTCTTCTACTTTTCCCACTTAAATTTCATTCTCCAATGATATTTTTCATATGAGCAAATATTAGGCTAAATTACAAAAAAAATACCTTGAAATTTGCCATTTGTTTAAAAAATACTTCTATCCTTTAAAAAGTTTCAATACTAATTACTACCCTTATCCTTTCATAAGCATTTCAAAAGTACTTTTGAGAAAATAGAAAGTTGACGTGATGTGACAATTAACGTGGAAGCTTCCCCACCTTTATTCTCTCTTTCCTCCATGCTTCTCCATTGTTTACTCAATTTTGTCCTCGTTCCCCCTTTTTAAAACGATAGCTTCTCCCACATTTTCTCTATTTTCAGATTTAATCCAGGCAAGACTTTCACGTTAATTGTCACGTCGTTTTTAATTCTGTTGAGTCAACTTTCCATTTAAAATATTAACGAATGTTTGCTGAAAAGGTATTTTTGAAACCTTTATGAAAGGTCAATGGTAGTATTGAAATTTTTGAAAGGATAGAGATATTCTTTAAACAATCATCGAGAGTGTTGATAATTATTTGCTAGCCTCTTCCTTTATGGAGGAATTTTATTAACTCAAAAAAGTAGGCCTGATCAGAAAGTAACTGACATTGTGTCGCCTTATAATAATAATGAAAGATAAATTACTCGAGATGGATATACCAAAATAATAACCTTAACAGCATCCACATTATATATATGAAGGTCGCGAAATTACTTGGTCTGCATGAAGCAGGAGCTCTAGCTAAAAGAGCAATACAGGTATGTTAATGACTTGATCTTTCCTCTAGTCACCACCCTCTTAGTTTAACAACAGCTAAAATATTATGAGATCGGTTGCAAGCAAAAACTGTGTGATATGATTGGTATATCGAAAGGACTCGACTCGACTTGTTAGAACTTTGATTAAATGTAACTTTGACTCGGAGCCAGGGGGGACACATATACTTTTCTTTAGATGCTTCTGTAATCCTAATACACATTCGACTCCACTCTAGTTTGGCTCAAGGATTGCGCGGGAATCAAACCGTAAGAACTTCGGGAGCAACCGAGCTGCTAATCAGGCCTCAGCAGGGAGTGCGAAGATCAAGCAGCATACGAGCAGC

mRNA sequence

ATGTGTGGAATCGCTTTGATTTTTTCTGGCGTTCGCCTCAATTTGTCACTTCTGCATGTAGACGCCAGATCGCCGTCTCTTCACCCAGTCCACGAGCAACTCGAGTTTCCTATAGATGGTCTTAAGGCGGCGTTGCGGAGAAGGGGACCCGATAGCTTAGGCGGAGTGAAGGTTTTTATCTCGTCGAAATCTTCTAGTCATGGCGAAGAAAATCTGTTTGAGTGCTTCATTGAGAGGGTTGATGATGGAGATGCATTCGAGAATCAAAATGATCGATACAAGATGGAAAATAGTTGCTTCTGTTCGGAAAATGGATTGGTTCTTCAATCAGAGAACAGTCATAGCGATCCCACTTCTGTTGCGGAGTTGTATTTAATAGGGGCTACGTTGCAGCTTAGAGGGCTTAACCCTATTGTTCAGCCACTGATGGATGCTTCCCGCAATATTCTTGTTTACAATGGCGAAATATTTGGAGGAATACCCATTGAGCGTGATGAAAATGATGCTGAAACACTTATGCAAGCTCTAGGGACTTGTTGCGCCAGCAATTTTAATTTAGATATAAGCACGTGTAACGGTGAAGGTAAGGGACAAAGCACAATCGTAGATATTCTTTCAAAGATTCAAGGGCCATGGTCAATCATCTACTGGCAGGATAGTGCAAAAACTTTGTGGTTTGGTCGCGATGCCTTTGGTAGGCGAAGTCTTCTTGTTCACTGGCCAACAGTGGAGGATTCTCGGTTTCTGTTGTCTTCTGCATCACCAGCTTCTTCCATATTTCACAGCTCTGGGCTTGATGATGATAATGGTACTTGTGAAGTGAGCTTCTGGGAAGAACTTCCATGTGGGATTTATAGCATCTCTTTTGATGCTCAGAGCAGGGACTGTTGCCTGGTCGGTGCAGTCAAAAAGCATGAGTGGACCAATGAGTCACTGAAAGAACTTGTTGAGTGGAAGAGAACTCGTATTGAACCTGAACCTGGAAATATCTCCGCCTCATATCTCAAGGATTTTTTGTCACAGCATGAATGCAACTCTGGATGTTTACATAACTTGCAATCCATATCAGGGAGTATTGAAGCTCCTGTTTCAACACCTGCGGAGATTGTGTTGGATGCTCTAAGAAAGTCTCTGATCCGGCGTACTTCACTTTACACAGTTTTCCAGGGCACAACATCTGCTTCCAGAAAAGCCGAGCTTGGTCCAGTGGCCATTCTTTTTTCTGGTGGATTGGATTCTATGATACTAGCAGCATTACTGGATGAGTGCCTAGATGCCTGTTATGAAATTGATCTTCTTAATGTCAGCTTTGATGGTTGTTCTGCACCTGATCGAATCTCTGCAAAGGCTGGAGTTCAGGAACTAAGAAGGATTGCACCTCTAAGAAGGCATTATTACGAGCTTTATCTTGGGTGGAAGCTGGTCGAGGTTGATGCTGATTTGTCAGATTTGACCTCTGAAACCAAGCATGTCATATCTCTAATAAATCCTGCAAATACGTACATGGACCTGAATATTGGAATTGCTTTGTGGCTGGCCTCTGGTGGCAATGGTTGGATTTACGAAGCAATTGACAAGGATGAGAAGTTTCATCGACGTGTTAAATACAAGTCTGAGGCCAAAATTCTCTTGGTTGGTTCTGGTGCTGATGAGCAATGTGCTGGCTATGGTAGGCATCGAACAAAATTTAGACAAGGGGGCTGGCATCTTTTAAACGAGGAAATGAAATTGGATATTCAGAGAATCTGGAAGAGAAATCTAGGGAGAGATGATAGATGTATTGCTGATAATGGCAAGGAGGCTAGATTTCCATTTTTGGATGAAGACGTTATAAATACATTGCTATCATTTCCTCTATGGGAGGTCACTGACCTTGAGAAACCTGTCGGAACCGGTGATAAGAAGATACTTAGGGAGGTCGCGAAATTACTTGGTCTGCATGAAGCAGGAGCTCTAGCTAAAAGAGCAATACAGTTTGGCTCAAGGATTGCGCGGGAATCAAACCGTAAGAACTTCGGGAGCAACCGAGCTGCTAATCAGGCCTCAGCAGGGAGTGCGAAGATCAAGCAGCATACGAGCAGC

Coding sequence (CDS)

ATGTGTGGAATCGCTTTGATTTTTTCTGGCGTTCGCCTCAATTTGTCACTTCTGCATGTAGACGCCAGATCGCCGTCTCTTCACCCAGTCCACGAGCAACTCGAGTTTCCTATAGATGGTCTTAAGGCGGCGTTGCGGAGAAGGGGACCCGATAGCTTAGGCGGAGTGAAGGTTTTTATCTCGTCGAAATCTTCTAGTCATGGCGAAGAAAATCTGTTTGAGTGCTTCATTGAGAGGGTTGATGATGGAGATGCATTCGAGAATCAAAATGATCGATACAAGATGGAAAATAGTTGCTTCTGTTCGGAAAATGGATTGGTTCTTCAATCAGAGAACAGTCATAGCGATCCCACTTCTGTTGCGGAGTTGTATTTAATAGGGGCTACGTTGCAGCTTAGAGGGCTTAACCCTATTGTTCAGCCACTGATGGATGCTTCCCGCAATATTCTTGTTTACAATGGCGAAATATTTGGAGGAATACCCATTGAGCGTGATGAAAATGATGCTGAAACACTTATGCAAGCTCTAGGGACTTGTTGCGCCAGCAATTTTAATTTAGATATAAGCACGTGTAACGGTGAAGGTAAGGGACAAAGCACAATCGTAGATATTCTTTCAAAGATTCAAGGGCCATGGTCAATCATCTACTGGCAGGATAGTGCAAAAACTTTGTGGTTTGGTCGCGATGCCTTTGGTAGGCGAAGTCTTCTTGTTCACTGGCCAACAGTGGAGGATTCTCGGTTTCTGTTGTCTTCTGCATCACCAGCTTCTTCCATATTTCACAGCTCTGGGCTTGATGATGATAATGGTACTTGTGAAGTGAGCTTCTGGGAAGAACTTCCATGTGGGATTTATAGCATCTCTTTTGATGCTCAGAGCAGGGACTGTTGCCTGGTCGGTGCAGTCAAAAAGCATGAGTGGACCAATGAGTCACTGAAAGAACTTGTTGAGTGGAAGAGAACTCGTATTGAACCTGAACCTGGAAATATCTCCGCCTCATATCTCAAGGATTTTTTGTCACAGCATGAATGCAACTCTGGATGTTTACATAACTTGCAATCCATATCAGGGAGTATTGAAGCTCCTGTTTCAACACCTGCGGAGATTGTGTTGGATGCTCTAAGAAAGTCTCTGATCCGGCGTACTTCACTTTACACAGTTTTCCAGGGCACAACATCTGCTTCCAGAAAAGCCGAGCTTGGTCCAGTGGCCATTCTTTTTTCTGGTGGATTGGATTCTATGATACTAGCAGCATTACTGGATGAGTGCCTAGATGCCTGTTATGAAATTGATCTTCTTAATGTCAGCTTTGATGGTTGTTCTGCACCTGATCGAATCTCTGCAAAGGCTGGAGTTCAGGAACTAAGAAGGATTGCACCTCTAAGAAGGCATTATTACGAGCTTTATCTTGGGTGGAAGCTGGTCGAGGTTGATGCTGATTTGTCAGATTTGACCTCTGAAACCAAGCATGTCATATCTCTAATAAATCCTGCAAATACGTACATGGACCTGAATATTGGAATTGCTTTGTGGCTGGCCTCTGGTGGCAATGGTTGGATTTACGAAGCAATTGACAAGGATGAGAAGTTTCATCGACGTGTTAAATACAAGTCTGAGGCCAAAATTCTCTTGGTTGGTTCTGGTGCTGATGAGCAATGTGCTGGCTATGGTAGGCATCGAACAAAATTTAGACAAGGGGGCTGGCATCTTTTAAACGAGGAAATGAAATTGGATATTCAGAGAATCTGGAAGAGAAATCTAGGGAGAGATGATAGATGTATTGCTGATAATGGCAAGGAGGCTAGATTTCCATTTTTGGATGAAGACGTTATAAATACATTGCTATCATTTCCTCTATGGGAGGTCACTGACCTTGAGAAACCTGTCGGAACCGGTGATAAGAAGATACTTAGGGAGGTCGCGAAATTACTTGGTCTGCATGAAGCAGGAGCTCTAGCTAAAAGAGCAATACAGTTTGGCTCAAGGATTGCGCGGGAATCAAACCGTAAGAACTTCGGGAGCAACCGAGCTGCTAATCAGGCCTCAGCAGGGAGTGCGAAGATCAAGCAGCATACGAGCAGC

Protein sequence

MCGIALIFSGVRLNLSLLHVDARSPSLHPVHEQLEFPIDGLKAALRRRGPDSLGGVKVFISSKSSSHGEENLFECFIERVDDGDAFENQNDRYKMENSCFCSENGLVLQSENSHSDPTSVAELYLIGATLQLRGLNPIVQPLMDASRNILVYNGEIFGGIPIERDENDAETLMQALGTCCASNFNLDISTCNGEGKGQSTIVDILSKIQGPWSIIYWQDSAKTLWFGRDAFGRRSLLVHWPTVEDSRFLLSSASPASSIFHSSGLDDDNGTCEVSFWEELPCGIYSISFDAQSRDCCLVGAVKKHEWTNESLKELVEWKRTRIEPEPGNISASYLKDFLSQHECNSGCLHNLQSISGSIEAPVSTPAEIVLDALRKSLIRRTSLYTVFQGTTSASRKAELGPVAILFSGGLDSMILAALLDECLDACYEIDLLNVSFDGCSAPDRISAKAGVQELRRIAPLRRHYYELYLGWKLVEVDADLSDLTSETKHVISLINPANTYMDLNIGIALWLASGGNGWIYEAIDKDEKFHRRVKYKSEAKILLVGSGADEQCAGYGRHRTKFRQGGWHLLNEEMKLDIQRIWKRNLGRDDRCIADNGKEARFPFLDEDVINTLLSFPLWEVTDLEKPVGTGDKKILREVAKLLGLHEAGALAKRAIQFGSRIARESNRKNFGSNRAANQASAGSAKIKQHTSS
Homology
BLAST of MS015239 vs. NCBI nr
Match: XP_022149042.1 (asparagine synthetase domain-containing protein 1 isoform X2 [Momordica charantia])

HSP 1 Score: 1369.8 bits (3544), Expect = 0.0e+00
Identity = 681/694 (98.13%), Postives = 682/694 (98.27%), Query Frame = 0

Query: 1   MCGIALIFSGVRLNLSLLHVDARSPSLHPVHEQLEFPIDGLKAALRRRGPDSLGGVKVFI 60
           MCGIALIFSGVRLNLSLLHVDARSPSLHPVHEQLEFPIDGLKAALRRRGPDSLGGVKVFI
Sbjct: 1   MCGIALIFSGVRLNLSLLHVDARSPSLHPVHEQLEFPIDGLKAALRRRGPDSLGGVKVFI 60

Query: 61  SSKSSSHGEENLFECFIERVDDGDAFENQNDRYKMENSCFCSENGLVLQSENSHSDPTSV 120
           SSKSSSHGEENLFECFIERVDDGDAFENQNDRYKMENSCFCSENGLVLQSENSHSDPTSV
Sbjct: 61  SSKSSSHGEENLFECFIERVDDGDAFENQNDRYKMENSCFCSENGLVLQSENSHSDPTSV 120

Query: 121 AELYLIGATLQLRGLNPIVQPLMDASRNILVYNGEIFGGIPIERDENDAETLMQALGTCC 180
           AELYLIGATLQLRGLNPIVQPLMDASRNILVYNGEIFGGIPIERDENDAETLMQALGTCC
Sbjct: 121 AELYLIGATLQLRGLNPIVQPLMDASRNILVYNGEIFGGIPIERDENDAETLMQALGTCC 180

Query: 181 ASNFNLDISTCNGEGKGQSTIVDILSKIQGPWSIIYWQDSAKTLWFGRDAFGRRSLLVHW 240
           ASNFNLDISTCNGEGKGQSTIVDILSKIQGPWSIIYWQD AKTLWFGRDAFGRRSLLVHW
Sbjct: 181 ASNFNLDISTCNGEGKGQSTIVDILSKIQGPWSIIYWQDRAKTLWFGRDAFGRRSLLVHW 240

Query: 241 PTVEDSRFLLSSASPASSIFHSSGLDDDNGTCEVSFWEELPCGIYSISFDAQSRDCCLVG 300
           PTVEDSRFLLSS SPASSIFHSSGLDDDNGTCEVSFWEELPCGIYSISFDAQSRDCCLVG
Sbjct: 241 PTVEDSRFLLSSVSPASSIFHSSGLDDDNGTCEVSFWEELPCGIYSISFDAQSRDCCLVG 300

Query: 301 AVKKHEWTNESLKELVEWKRTRIEPEPGNISASYLKDFLSQHECNSGCLHNLQSISGSIE 360
           AVKKHEWTNESLKELVEWKRTRIEPEPGNISASYLKDFLSQHECNSGCLHNLQSISGS E
Sbjct: 301 AVKKHEWTNESLKELVEWKRTRIEPEPGNISASYLKDFLSQHECNSGCLHNLQSISGSTE 360

Query: 361 APVSTPAEIVLDALRKSLIRRTSLYTVFQGTTSASRKAELGPVAILFSGGLDSMILAALL 420
           APVSTPAEIVLDALRKSLIRRTSLYTVFQGTTSASRKAELGPVAILFSGGLDSMILAALL
Sbjct: 361 APVSTPAEIVLDALRKSLIRRTSLYTVFQGTTSASRKAELGPVAILFSGGLDSMILAALL 420

Query: 421 DECLDACYEIDLLNVSFDGCSAPDRISAKAGVQELRRIAPLRRHYYELYLGWKLVEVDAD 480
           DECLDACYEIDLLNVSFDGCSAPDRISAKAGVQELRRIAPLRR        WKLVEVDAD
Sbjct: 421 DECLDACYEIDLLNVSFDGCSAPDRISAKAGVQELRRIAPLRR--------WKLVEVDAD 480

Query: 481 LSDLTSETKHVISLINPANTYMDLNIGIALWLASGGNGWIYEAIDKDEKFHRRVKYKSEA 540
           LSDLTSETKHV+SLINPANTYMDLNIGIALWLASGGNGWIYEAIDKDEKFHRRVKYKSEA
Sbjct: 481 LSDLTSETKHVMSLINPANTYMDLNIGIALWLASGGNGWIYEAIDKDEKFHRRVKYKSEA 540

Query: 541 KILLVGSGADEQCAGYGRHRTKFRQGGWHLLNEEMKLDIQRIWKRNLGRDDRCIADNGKE 600
           KILLVGSGADEQCAGYGRHRTKFRQGGWHLLNEEMKLDIQRIWKRNLGRDDRCIADNGKE
Sbjct: 541 KILLVGSGADEQCAGYGRHRTKFRQGGWHLLNEEMKLDIQRIWKRNLGRDDRCIADNGKE 600

Query: 601 ARFPFLDEDVINTLLSFPLWEVTDLEKPVGTGDKKILREVAKLLGLHEAGALAKRAIQFG 660
           ARFPFLDEDVINTLLSFPLWEVTDLEKPVGTGDKKILREVAKLLGLHEAGALAKRAIQFG
Sbjct: 601 ARFPFLDEDVINTLLSFPLWEVTDLEKPVGTGDKKILREVAKLLGLHEAGALAKRAIQFG 660

Query: 661 SRIARESNRKNFGSNRAANQASAGSAKIKQHTSS 695
           SRIARESNRKNFGSNRAANQASAGS KIKQHTSS
Sbjct: 661 SRIARESNRKNFGSNRAANQASAGSVKIKQHTSS 686

BLAST of MS015239 vs. NCBI nr
Match: XP_022149041.1 (asparagine synthetase domain-containing protein 1 isoform X1 [Momordica charantia])

HSP 1 Score: 1310.4 bits (3390), Expect = 0.0e+00
Identity = 647/660 (98.03%), Postives = 648/660 (98.18%), Query Frame = 0

Query: 1   MCGIALIFSGVRLNLSLLHVDARSPSLHPVHEQLEFPIDGLKAALRRRGPDSLGGVKVFI 60
           MCGIALIFSGVRLNLSLLHVDARSPSLHPVHEQLEFPIDGLKAALRRRGPDSLGGVKVFI
Sbjct: 1   MCGIALIFSGVRLNLSLLHVDARSPSLHPVHEQLEFPIDGLKAALRRRGPDSLGGVKVFI 60

Query: 61  SSKSSSHGEENLFECFIERVDDGDAFENQNDRYKMENSCFCSENGLVLQSENSHSDPTSV 120
           SSKSSSHGEENLFECFIERVDDGDAFENQNDRYKMENSCFCSENGLVLQSENSHSDPTSV
Sbjct: 61  SSKSSSHGEENLFECFIERVDDGDAFENQNDRYKMENSCFCSENGLVLQSENSHSDPTSV 120

Query: 121 AELYLIGATLQLRGLNPIVQPLMDASRNILVYNGEIFGGIPIERDENDAETLMQALGTCC 180
           AELYLIGATLQLRGLNPIVQPLMDASRNILVYNGEIFGGIPIERDENDAETLMQALGTCC
Sbjct: 121 AELYLIGATLQLRGLNPIVQPLMDASRNILVYNGEIFGGIPIERDENDAETLMQALGTCC 180

Query: 181 ASNFNLDISTCNGEGKGQSTIVDILSKIQGPWSIIYWQDSAKTLWFGRDAFGRRSLLVHW 240
           ASNFNLDISTCNGEGKGQSTIVDILSKIQGPWSIIYWQD AKTLWFGRDAFGRRSLLVHW
Sbjct: 181 ASNFNLDISTCNGEGKGQSTIVDILSKIQGPWSIIYWQDRAKTLWFGRDAFGRRSLLVHW 240

Query: 241 PTVEDSRFLLSSASPASSIFHSSGLDDDNGTCEVSFWEELPCGIYSISFDAQSRDCCLVG 300
           PTVEDSRFLLSS SPASSIFHSSGLDDDNGTCEVSFWEELPCGIYSISFDAQSRDCCLVG
Sbjct: 241 PTVEDSRFLLSSVSPASSIFHSSGLDDDNGTCEVSFWEELPCGIYSISFDAQSRDCCLVG 300

Query: 301 AVKKHEWTNESLKELVEWKRTRIEPEPGNISASYLKDFLSQHECNSGCLHNLQSISGSIE 360
           AVKKHEWTNESLKELVEWKRTRIEPEPGNISASYLKDFLSQHECNSGCLHNLQSISGS E
Sbjct: 301 AVKKHEWTNESLKELVEWKRTRIEPEPGNISASYLKDFLSQHECNSGCLHNLQSISGSTE 360

Query: 361 APVSTPAEIVLDALRKSLIRRTSLYTVFQGTTSASRKAELGPVAILFSGGLDSMILAALL 420
           APVSTPAEIVLDALRKSLIRRTSLYTVFQGTTSASRKAELGPVAILFSGGLDSMILAALL
Sbjct: 361 APVSTPAEIVLDALRKSLIRRTSLYTVFQGTTSASRKAELGPVAILFSGGLDSMILAALL 420

Query: 421 DECLDACYEIDLLNVSFDGCSAPDRISAKAGVQELRRIAPLRRHYYELYLGWKLVEVDAD 480
           DECLDACYEIDLLNVSFDGCSAPDRISAKAGVQELRRIAPLRR        WKLVEVDAD
Sbjct: 421 DECLDACYEIDLLNVSFDGCSAPDRISAKAGVQELRRIAPLRR--------WKLVEVDAD 480

Query: 481 LSDLTSETKHVISLINPANTYMDLNIGIALWLASGGNGWIYEAIDKDEKFHRRVKYKSEA 540
           LSDLTSETKHV+SLINPANTYMDLNIGIALWLASGGNGWIYEAIDKDEKFHRRVKYKSEA
Sbjct: 481 LSDLTSETKHVMSLINPANTYMDLNIGIALWLASGGNGWIYEAIDKDEKFHRRVKYKSEA 540

Query: 541 KILLVGSGADEQCAGYGRHRTKFRQGGWHLLNEEMKLDIQRIWKRNLGRDDRCIADNGKE 600
           KILLVGSGADEQCAGYGRHRTKFRQGGWHLLNEEMKLDIQRIWKRNLGRDDRCIADNGKE
Sbjct: 541 KILLVGSGADEQCAGYGRHRTKFRQGGWHLLNEEMKLDIQRIWKRNLGRDDRCIADNGKE 600

Query: 601 ARFPFLDEDVINTLLSFPLWEVTDLEKPVGTGDKKILREVAKLLGLHEAGALAKRAIQFG 660
           ARFPFLDEDVINTLLSFPLWEVTDLEKPVGTGDKKILREVAKLLGLHEAGALAKRAIQ G
Sbjct: 601 ARFPFLDEDVINTLLSFPLWEVTDLEKPVGTGDKKILREVAKLLGLHEAGALAKRAIQGG 652

BLAST of MS015239 vs. NCBI nr
Match: XP_038892575.1 (asparagine synthetase domain-containing protein 1 [Benincasa hispida])

HSP 1 Score: 1159.8 bits (2999), Expect = 0.0e+00
Identity = 578/692 (83.53%), Postives = 620/692 (89.60%), Query Frame = 0

Query: 1   MCGIALIFSGVRLNLSLLHVDARSPSLHPVHEQLEFPIDGLKAALRRRGPDSLGGVKVFI 60
           MCGIALI SG+RL+LS LHVDA+SPS HPVHEQL+F ID LKAALRRRGPDSLGGVK+FI
Sbjct: 1   MCGIALIVSGIRLDLSSLHVDAKSPSPHPVHEQLQFSIDDLKAALRRRGPDSLGGVKIFI 60

Query: 61  SSKSSSHGEENLFECFIERVDDGDAFENQNDRYKMENSCFCSENGLVLQSENSHSDPTSV 120
           SSKSS+ GEE  FEC IERVDDG A ++QND+Y+M+  CF SENG + QSENS S P S 
Sbjct: 61  SSKSSNPGEEQQFECSIERVDDGVAVQSQNDQYEMKIGCFRSENGWIPQSENSCSSPISG 120

Query: 121 AELYLIGATLQLRGLNPIVQPLMDASRNILVYNGEIFGGIPIERDENDAETLMQALGTCC 180
           AE YL+GATLQLRG+NPIVQPLMDASRNILVYNGEIFGGI +E   NDAETLM AL  CC
Sbjct: 121 AEFYLLGATLQLRGINPIVQPLMDASRNILVYNGEIFGGIHVEYQGNDAETLMLALQNCC 180

Query: 181 ASNFNLDISTCNGEGKGQSTIVDILSKIQGPWSIIYWQDSAKTLWFGRDAFGRRSLLVHW 240
           A NF+L  +TC GE K + TIVDILS +QGPWSIIYWQDSAKTLWFGRDAFGRRSLLVHW
Sbjct: 181 ACNFSLGTNTCEGECKRERTIVDILSIVQGPWSIIYWQDSAKTLWFGRDAFGRRSLLVHW 240

Query: 241 PTVEDSRFLLSSASPASSIFHSSGLDDDNGTCEVSFWEELPCGIYSISFDAQSRDCCLVG 300
           PTVEDSRFLLSS SPASS+FH +GL+DDNGTCEVSFWEELPCGIYSISFDAQ  D  LVG
Sbjct: 241 PTVEDSRFLLSSVSPASSMFHGAGLEDDNGTCEVSFWEELPCGIYSISFDAQRTDGFLVG 300

Query: 301 AVKKHEWTNESLKELVEWKRTRIEPEPGNISASYLKDFLSQHECNSGCLHNLQSISGSIE 360
            VK+HEWTNESLKELVEWKRTRI PEP +IS  YLKD LSQ+E +  CL NL S+SGSI+
Sbjct: 301 EVKRHEWTNESLKELVEWKRTRIVPEPRDISTPYLKDLLSQYESHLACLPNLLSVSGSIQ 360

Query: 361 APVSTPAEIVLDALRKSLIRRTSLYTVFQGTTSASRKAELGPVAILFSGGLDSMILAALL 420
           APVSTPAEI+L+ALRKSLIRRTSLY +FQGTTSASRK ELGPVAILFSGGLDSMILAALL
Sbjct: 361 APVSTPAEILLEALRKSLIRRTSLYRIFQGTTSASRKTELGPVAILFSGGLDSMILAALL 420

Query: 421 DECLDACYEIDLLNVSFDGCSAPDRISAKAGVQELRRIAPLRRHYYELYLGWKLVEVDAD 480
           DECLD CYEIDLLNVSFDGCSAPDRISAKAGV+EL RIAPLRR        WKLVE+DAD
Sbjct: 421 DECLDTCYEIDLLNVSFDGCSAPDRISAKAGVKELSRIAPLRR--------WKLVEIDAD 480

Query: 481 LSDLTSETKHVISLINPANTYMDLNIGIALWLASGGNGWIYEAIDKDEKFHRRVKYKSEA 540
           LS+LTSETKHV+SLINPANTYMDLNIGIALWLASGGNGWIYE+IDKDEKFH+RVKYKSEA
Sbjct: 481 LSNLTSETKHVMSLINPANTYMDLNIGIALWLASGGNGWIYESIDKDEKFHQRVKYKSEA 540

Query: 541 KILLVGSGADEQCAGYGRHRTKFRQGGWHLLNEEMKLDIQRIWKRNLGRDDRCIADNGKE 600
           KILLVGSGADEQCAGYGRHRTK+RQGGWHLLNEEMKLDIQRIWKRNLGRDDRCIADNGKE
Sbjct: 541 KILLVGSGADEQCAGYGRHRTKYRQGGWHLLNEEMKLDIQRIWKRNLGRDDRCIADNGKE 600

Query: 601 ARFPFLDEDVINTLLSFPLWEVTDLEKPVGTGDKKILREVAKLLGLHEAGALAKRAIQFG 660
           ARFPFLDEDVI+ LL+FPLWEVTDLEKP GTGDKKILREVAKLLGLHEAGAL KRAIQFG
Sbjct: 601 ARFPFLDEDVIHILLAFPLWEVTDLEKPAGTGDKKILREVAKLLGLHEAGALPKRAIQFG 660

Query: 661 SRIARESNRKNFGSNRAANQASAGSAKIKQHT 693
           SRIARESNRKNFGSNRAANQASAGS K  + +
Sbjct: 661 SRIARESNRKNFGSNRAANQASAGSVKFNRQS 684

BLAST of MS015239 vs. NCBI nr
Match: KAA0034476.1 (asparagine synthetase domain-containing protein 1 isoform X2 [Cucumis melo var. makuwa])

HSP 1 Score: 1157.9 bits (2994), Expect = 0.0e+00
Identity = 572/693 (82.54%), Postives = 619/693 (89.32%), Query Frame = 0

Query: 1   MCGIALIFSGVRLNLSLLHVDARSPSLHPVHEQLEFPIDGLKAALRRRGPDSLGGVKVFI 60
           MCGIALI SG+RL+LS LHVDAR  S   VHEQLEF ID LKA LRRRGPDSLGGVKV +
Sbjct: 1   MCGIALIVSGIRLDLSSLHVDARPLSPSSVHEQLEFSIDDLKAVLRRRGPDSLGGVKVSL 60

Query: 61  SSKSSSHGEENLFECFIERVDDGDAFENQNDRYKMENSCFCSENGLVLQSENSHSDPTSV 120
           SSKSS   EE   ECFIERVDD  AF+NQND+Y+MEN  FCSENG +LQSEN  S+P S 
Sbjct: 61  SSKSSYPSEEQQLECFIERVDDCVAFQNQNDQYEMENGRFCSENGCILQSENGCSNPISG 120

Query: 121 AELYLIGATLQLRGLNPIVQPLMDASRNILVYNGEIFGGIPIERDENDAETLMQALGTCC 180
             LYL+GATLQLRG+NPIVQPLMDASRNILVYNGEIFGG+ +E  ENDAE+LM AL  CC
Sbjct: 121 TGLYLLGATLQLRGVNPIVQPLMDASRNILVYNGEIFGGLHVECHENDAESLMLALRNCC 180

Query: 181 ASNFNLDISTCNGEGKGQSTIVDILSKIQGPWSIIYWQDSAKTLWFGRDAFGRRSLLVHW 240
           + NF+LD++ C  E K + TIVD LSKIQGPWSIIYWQDSAKTLWFGRDAFGRRSLLVHW
Sbjct: 181 SCNFSLDMNACEDECKRERTIVDTLSKIQGPWSIIYWQDSAKTLWFGRDAFGRRSLLVHW 240

Query: 241 PTVEDSRFLLSSASPASSIFHSSGLDDDNGTCEVSFWEELPCGIYSISFDAQSRDCCLVG 300
           PTVEDSRFLLSS SPASS+F  SGL+ D+GTC+VSFWEELPCGIYS+SFDAQ  D C+VG
Sbjct: 241 PTVEDSRFLLSSVSPASSMFQDSGLEVDSGTCQVSFWEELPCGIYSVSFDAQKTDGCVVG 300

Query: 301 AVKKHEWTNESLKELVEWKRTRIEPEPGNISASYLKDFLSQHECNSGCLHNLQSISGSIE 360
            V++HEWTNESLKELVEWKRTRIEPE G+IS SY KD LSQHE +S CLHNLQS  G+ +
Sbjct: 301 EVRRHEWTNESLKELVEWKRTRIEPEHGDISTSYHKDCLSQHESHSTCLHNLQSALGNFQ 360

Query: 361 APVSTPAEIVLDALRKSLIRRTSLYTVFQGTTSASRKAELGPVAILFSGGLDSMILAALL 420
           APVSTPAEI+LDALRKSL+RRTS YT+FQ TTSASRKAELGPVAILFSGGLDSMILAALL
Sbjct: 361 APVSTPAEILLDALRKSLVRRTSFYTIFQATTSASRKAELGPVAILFSGGLDSMILAALL 420

Query: 421 DECLDACYEIDLLNVSFDGCSAPDRISAKAGVQELRRIAPLRRHY-YELYLGWKLVEVDA 480
           DECLD CYE+DLLNVSFDGCSAPDRISAKAGV+EL RIAPLRRHY  ELYL WKLVE+DA
Sbjct: 421 DECLDPCYEVDLLNVSFDGCSAPDRISAKAGVKELSRIAPLRRHYIIELYLKWKLVEIDA 480

Query: 481 DLSDLTSETKHVISLINPANTYMDLNIGIALWLASGGNGWIYEAIDKDEKFHRRVKYKSE 540
           D+SDLTSETKHV+SLINPANTYMDLNIGIALWLASGGNGWIYE++D+DEKFH+RVKYKSE
Sbjct: 481 DMSDLTSETKHVMSLINPANTYMDLNIGIALWLASGGNGWIYESVDEDEKFHQRVKYKSE 540

Query: 541 AKILLVGSGADEQCAGYGRHRTKFRQGGWHLLNEEMKLDIQRIWKRNLGRDDRCIADNGK 600
           AKILLVGSGADEQCAGYGRHRTK+RQGGWHLLNEEMKLDIQRIWKRNLGRDDRCIADNGK
Sbjct: 541 AKILLVGSGADEQCAGYGRHRTKYRQGGWHLLNEEMKLDIQRIWKRNLGRDDRCIADNGK 600

Query: 601 EARFPFLDEDVINTLLSFPLWEVTDLEKPVGTGDKKILREVAKLLGLHEAGALAKRAIQF 660
           EARFPFLDEDVI TLL+FPLWEVTDLEKP GTGDKKILREVAKLLGLHEAG L KRAIQF
Sbjct: 601 EARFPFLDEDVIRTLLAFPLWEVTDLEKPAGTGDKKILREVAKLLGLHEAGVLPKRAIQF 660

Query: 661 GSRIARESNRKNFGSNRAANQASAGSAKIKQHT 693
           GSRIARESNRKNFGSNRAANQA+AGS K  + +
Sbjct: 661 GSRIARESNRKNFGSNRAANQANAGSVKFNKQS 693

BLAST of MS015239 vs. NCBI nr
Match: XP_022957237.1 (asparagine synthetase domain-containing protein 1 isoform X1 [Cucurbita moschata] >KAG7032257.1 Asparagine synthetase domain-containing protein 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1147.1 bits (2966), Expect = 0.0e+00
Identity = 572/692 (82.66%), Postives = 619/692 (89.45%), Query Frame = 0

Query: 1   MCGIALIFSGVRLNLSLLHVDARSPSLHPVHEQLEFPIDGLKAALRRRGPDSLGGVKVFI 60
           MCGIAL+ SG RL+LS LHVDAR+PS HPVHE LEF ID LKAALRRRGPDSLG VKVFI
Sbjct: 1   MCGIALVVSGCRLDLSSLHVDARAPSPHPVHEHLEFSIDDLKAALRRRGPDSLGEVKVFI 60

Query: 61  SSKSSSHGEENLFECFIERVDDGDAFENQNDRYKMENSCFCSENGLVLQSENSHSDPTSV 120
           SS  S+ GEE  FECF+ERVD G AF+NQND+++M        NG +LQS  S S P S 
Sbjct: 61  SSICSNPGEEQQFECFVERVDCGVAFQNQNDQHEM-------ANGRILQSHASCSSPISG 120

Query: 121 AELYLIGATLQLRGLNPIVQPLMDASRNILVYNGEIFGGIPIERDENDAETLMQALGTCC 180
           A+   +GATLQLRGLNPIVQPLMDASRN+LVYNGEIFGGI +E  +NDAE LMQALG CC
Sbjct: 121 AQFCFLGATLQLRGLNPIVQPLMDASRNVLVYNGEIFGGIHVEYHQNDAEALMQALGNCC 180

Query: 181 ASNFNLDISTCNGEGKGQSTIVDILSKIQGPWSIIYWQDSAKTLWFGRDAFGRRSLLVHW 240
           ASN +LD +TC GE K +STIVD+LS IQGPW+IIY+ DSAKTLWFGRDAFGRRSLLVHW
Sbjct: 181 ASNVSLDTNTCVGECKRESTIVDVLSNIQGPWAIIYFADSAKTLWFGRDAFGRRSLLVHW 240

Query: 241 PTVEDSRFLLSSASPASSIFHSSGLDDDNGTCEVSFWEELPCGIYSISFDAQSRDCCLVG 300
           PTVEDSRFLLSS SPASS+FH SG D+DNGTCEVSFWEELPCGIYSISFDA+S D CLVG
Sbjct: 241 PTVEDSRFLLSSVSPASSMFHDSGHDNDNGTCEVSFWEELPCGIYSISFDARSTDGCLVG 300

Query: 301 AVKKHEWTNESLKELVEWKRTRIEPEPGNISASYLKDFLSQHECNSGCLHNLQSISGSIE 360
            VK+HEWTNESLKEL+EWKRT I P+PG+IS+SY +D LSQHE NS CLHNLQS+S  I+
Sbjct: 301 EVKRHEWTNESLKELIEWKRTCIVPKPGDISSSYHEDLLSQHESNSACLHNLQSVSEGIQ 360

Query: 361 APVSTPAEIVLDALRKSLIRRTSLYTVFQGTTSASRKAELGPVAILFSGGLDSMILAALL 420
           APVSTPAEI+LDALRKSLIRRTSLYT+FQGTTSASRKAELGPVAILFSGGLDSMILAALL
Sbjct: 361 APVSTPAEILLDALRKSLIRRTSLYTIFQGTTSASRKAELGPVAILFSGGLDSMILAALL 420

Query: 421 DECLDACYEIDLLNVSFDGCSAPDRISAKAGVQELRRIAPLRRHYYELYLGWKLVEVDAD 480
           DECLDACYEIDLLNVSFDGCSAPDRISAKAGVQEL RIAPLRR        WKLVE+DAD
Sbjct: 421 DECLDACYEIDLLNVSFDGCSAPDRISAKAGVQELTRIAPLRR--------WKLVEIDAD 480

Query: 481 LSDLTSETKHVISLINPANTYMDLNIGIALWLASGGNGWIYEAIDKDEKFHRRVKYKSEA 540
           LS+LTSETKHV+SLINPANTYMDLNIGIALWLASGG+GWIYE+IDKD+KFHRRVKYKS+A
Sbjct: 481 LSNLTSETKHVMSLINPANTYMDLNIGIALWLASGGDGWIYESIDKDDKFHRRVKYKSQA 540

Query: 541 KILLVGSGADEQCAGYGRHRTKFRQGGWHLLNEEMKLDIQRIWKRNLGRDDRCIADNGKE 600
           KILLVGSGADEQCAGYGRHRTK+RQGGWHLLNEEMKLDIQRIWKRNLGRDDRCIADNGKE
Sbjct: 541 KILLVGSGADEQCAGYGRHRTKYRQGGWHLLNEEMKLDIQRIWKRNLGRDDRCIADNGKE 600

Query: 601 ARFPFLDEDVINTLLSFPLWEVTDLEKPVGTGDKKILREVAKLLGLHEAGALAKRAIQFG 660
           ARFPFLDEDVI+TLL+FPLWEVT+LEKP GTGDKKILREVAKLLGLHEAGAL KRAIQFG
Sbjct: 601 ARFPFLDEDVIHTLLAFPLWEVTNLEKPAGTGDKKILREVAKLLGLHEAGALPKRAIQFG 660

Query: 661 SRIARESNRKNFGSNRAANQASAGSAKIKQHT 693
           SRIARESNRKNFGSNRAANQASAGS K  +H+
Sbjct: 661 SRIARESNRKNFGSNRAANQASAGSVKFNKHS 677

BLAST of MS015239 vs. ExPASy Swiss-Prot
Match: Q8JFW1 (Asparagine synthetase domain-containing protein 1 OS=Danio rerio OX=7955 GN=asnsd1 PE=2 SV=1)

HSP 1 Score: 301.2 bits (770), Expect = 3.0e-80
Identity = 209/606 (34.49%), Postives = 308/606 (50.83%), Query Frame = 0

Query: 130 LQLRG-LNPIVQPLMDASRNILVYNGEIFGGIPIERDENDAETLMQALGTCCASNFNLDI 189
           L +RG L P  QPL D + N+L++NGE+FGG+ +E++END E L+  L    ++      
Sbjct: 52  LHMRGCLTP--QPLQDDTGNMLLWNGEVFGGLKLEQEENDTEVLLHQLSMATSA------ 111

Query: 190 STCNGEGKGQSTIVDILSKIQGPWSIIYWQDSAKTLWFGRDAFGRRSLLVHWPTVEDSRF 249
                     S IV +LS+++GPW+ IY+Q S + +WFGRD FGRRSLL  +   E + F
Sbjct: 112 ----------SDIVCLLSQLEGPWAFIYYQKSERCIWFGRDFFGRRSLLWKF-NPERASF 171

Query: 250 LLSSASPASSIFHSSGLDDDNGTCEVSFWEEL-PCGIYSISFDAQSRDCCLVGAVKKHEW 309
            L+S +       S   DD       S W+E+ P G+Y       S D   +   + + W
Sbjct: 172 TLASVA-------SQPADDSQ-----SQWQEVPPGGVYRFDLTDTSLDGTFI--FELYPW 231

Query: 310 TNESLKELVEWKRTRIEPEP------GNISASYLKDFL-------------SQHECNSGC 369
              S  + +E    + E  P       N S  YL   +               H  +   
Sbjct: 232 IFHSDDKPIESPELKCEGLPRCVSVNTNSSGLYLTSPVCPMNTSISSLTTEQNHNASQTS 291

Query: 370 LHNLQSISGSIE--APVSTPAEIVLDALRKSLIRRTSLYTVFQGTTSASRKAELGPVAIL 429
           + NL++   + +  A VS   +++ +A+RK +      Y   +     S       +AIL
Sbjct: 292 VENLKAFLTNTDKKAMVSALIDVLSEAVRKRV-----QYLPARDALEVSLNQTKADIAIL 351

Query: 430 FSGGLDSMILAALLDECLDACYEIDLLNVSF-------------------DGC------- 489
           FSGG+DSMILAAL D  + A   IDLLNV+F                   + C       
Sbjct: 352 FSGGIDSMILAALTDRHVPADKPIDLLNVAFKMQEAKMPPKSMKKGKNKKNDCDDADKTQ 411

Query: 490 ---------SAPDRISAKAGVQELRRIAPLRRHYYELYLGWKLVEVDADLSDLTS-ETKH 549
                    + PDRI+ +AG+QEL+ ++P R+        W  +E++    +L     K 
Sbjct: 412 LDQQKFNPFNVPDRITGRAGLQELKNLSPKRQ--------WNFIEINVTQEELVEMRQKR 471

Query: 550 VISLINPANTYMDLNIGIALWLASGGNGWIYEAIDKDEKFHRRVKYKSEAKILLVGSGAD 609
           +  L++P +T +D ++G A+W A+ G G I EA+D++        Y SEAK++L G GAD
Sbjct: 472 ICHLVHPLDTVLDDSLGCAIWFAARGIGVINEAVDEE-------LYISEAKVVLTGIGAD 531

Query: 610 EQCAGYGRHRTKFRQGGWHLLNEEMKLDIQRIWKRNLGRDDRCIADNGKEARFPFLDEDV 669
           EQ AGY RHR +++  G   L  E+ +++ RI  RNLGRDDR I D+GKEARFP+LDEDV
Sbjct: 532 EQLAGYSRHRVRYKHSGLEGLVRELAMELGRISSRNLGRDDRIIGDHGKEARFPYLDEDV 591

Query: 670 INTLLSFPLWEVTDLEKPVGTGDKKILREVAKLLGLHEAGALAKRAIQFGSRIARESNRK 677
           ++ L   P+ E  DL  P G G+K +LR  A  LGL  +  L KRA+QFGSRIA+  +  
Sbjct: 592 VSFLNGLPVSEKADLSLPRGVGEKLLLRLAAVELGLGLSALLPKRAMQFGSRIAKLEDNH 604

BLAST of MS015239 vs. ExPASy Swiss-Prot
Match: Q0V8E4 (Asparagine synthetase domain-containing protein 1 OS=Bos taurus OX=9913 GN=ASNSD1 PE=2 SV=1)

HSP 1 Score: 299.3 bits (765), Expect = 1.1e-79
Identity = 216/625 (34.56%), Postives = 310/625 (49.60%), Query Frame = 0

Query: 127 GATLQLRGLNPIVQPLMDASRNILVYNGEIFGGIPIERDENDAETLMQALGTCCASNFNL 186
           G  L LRGL    QP+ D   N+ ++NGEIF GI +E +END + +   L +C       
Sbjct: 51  GHVLHLRGL-LTAQPVEDERGNVFLWNGEIFSGIKVEAEENDTQIMFHYLSSC------- 110

Query: 187 DISTCNGEGKGQSTIVDILSKIQGPWSIIYWQDSAKTLWFGRDAFGRRSLLVHWPTVEDS 246
                    K +S I+ + SK+QGPWS IY+Q S+ +LWFGRD FGRRSLL H+  +  S
Sbjct: 111 ---------KNESDILSLFSKVQGPWSFIYYQASSHSLWFGRDFFGRRSLLWHFSNLGKS 170

Query: 247 RFLLSSASPASSIFHSSGLDDDNGTCEVSFWEELPC-GIYSISFDAQSRDCCLVGAVKKH 306
            F LSS         +SG+ D         W+E+P  GI+ I   + S    +V  +K +
Sbjct: 171 -FCLSSVGT-----QASGVTDQ--------WQEVPASGIFRIDLKSASISQSVV--LKLY 230

Query: 307 EWT----NESLKELVEWKRTRIEPE-------PGNISASYLKD-------FLSQHECNSG 366
            W      + ++E V    T I  +         N +  YLKD        L Q    + 
Sbjct: 231 PWKYSSGGDDIRECVHNSLTEISADLPTFVLVAANEAKLYLKDPVVPLNMALPQAAFETH 290

Query: 367 CLHNLQS-----------ISGSIEAPVSTPAEIVLDALRKSL--IRRTSLYTVFQGTTSA 426
           C    +S             G  +  V     ++  A+++ +  + R       +   ++
Sbjct: 291 CSSISRSPLTRETLRVFLTDGHTKEVVQQFIGVLSTAVKRRVLCLPRDENLAPSEVLKTS 350

Query: 427 SRKAELGPVAILFSGGLDSMILAALLDECLDACYEIDLLNVSF----------------- 486
           +RKA    VA+LFSGG+DSM++AAL D  +     IDLLNV+F                 
Sbjct: 351 NRKAN---VAVLFSGGIDSMVIAALADHHIPLDEPIDLLNVAFMTKEKTIPVNFNKKGRK 410

Query: 487 -------------------------DGCSAPDRISAKAGVQELRRIAPLRRHYYELYLGW 546
                                    +  S PDR++ +AG++EL+   P R         W
Sbjct: 411 QANHCEMPSEEFSKHAAATAAASPGEQLSVPDRVTGRAGLKELQAANPSRI--------W 470

Query: 547 KLVEVDADLSDLTSETKHVIS-LINPANTYMDLNIGIALWLASGGNGWIYEAIDKDEKFH 606
             VE++  L +L    +  IS LI P +T +D +IG A+W AS G GW+   + +D    
Sbjct: 471 NFVEINVSLEELQRLRRTRISHLIQPLDTVLDDSIGCAVWFASRGAGWL---VTQD---- 530

Query: 607 RRVKYKSEAKILLVGSGADEQCAGYGRHRTKFRQGGWHLLNEEMKLDIQRIWKRNLGRDD 666
               Y+S AK++L G GADEQ AGY RH+ +F   G   LN+E+++++ RI  RNLGRDD
Sbjct: 531 GAQPYQSSAKVVLTGIGADEQLAGYSRHQVRFLAHGLEGLNKEIEMELGRISSRNLGRDD 590

Query: 667 RCIADNGKEARFPFLDEDVINTLLSFPLWEVTDLEKPVGTGDKKILREVAKLLGLHEAGA 677
           R I+D+GKEARFPFLDE+V++ L S P+WE  +L  P G G+K ILR  A  LGL  +  
Sbjct: 591 RVISDHGKEARFPFLDENVVSFLNSLPVWEKANLTLPRGIGEKLILRLAAVELGLTASAL 624

BLAST of MS015239 vs. ExPASy Swiss-Prot
Match: Q8BFS9 (Asparagine synthetase domain-containing protein 1 OS=Mus musculus OX=10090 GN=Asnsd1 PE=2 SV=1)

HSP 1 Score: 296.2 bits (757), Expect = 9.6e-79
Identity = 214/624 (34.29%), Postives = 301/624 (48.24%), Query Frame = 0

Query: 127 GATLQLRGLNPIVQPLMDASRNILVYNGEIFGGIPIERDENDAETLMQALGTCCASNFNL 186
           G  L LRG+  I QP+ D   N+ ++NGE+F G+ +E ++ND + +  +L  C       
Sbjct: 51  GHVLHLRGVLTI-QPVEDEHGNVFLWNGEVFNGVKVEAEDNDTQVMFNSLSAC------- 110

Query: 187 DISTCNGEGKGQSTIVDILSKIQGPWSIIYWQDSAKTLWFGRDAFGRRSLLVHWPTVEDS 246
                    K +S I+ + SK+QGPWS IY+Q S+  LWFGRD FGRRSLL  +  +  S
Sbjct: 111 ---------KNESEILLLFSKVQGPWSFIYYQASSHHLWFGRDFFGRRSLLWQFSNLGKS 170

Query: 247 RFLLSSASPASSIFHSSGLDDDNGTCEVSFWEELPC-GIYSISFDAQSRDCCLVGAVKKH 306
            F LSS    + ++   G+ D         W+E+P  GI+ I  ++ +    ++  +K +
Sbjct: 171 -FCLSSV--GTQVY---GVADQ--------WQEVPASGIFQIDLNSAAVSRSVI--LKLY 230

Query: 307 EWTNESLKELVEWKRTRIEPEPGNISASY-----------------LKDFLSQHECNSGC 366
            W   S +++ E     +   P  +                     L   L +      C
Sbjct: 231 PWRYISKEDIAEECGNDLTQTPAGLPEFVSVVINEANLYLSKPVVPLNKKLPESPLEIQC 290

Query: 367 LHNLQSISGSIEA---------------------PVSTPAEIVLDALRKSLIRRTSLYTV 426
             N  S SG+ E                       VS    I+  A  ++L  +  L T 
Sbjct: 291 -RNSSSTSGTRETLEVFLTDEHTKKIVQQFIAILNVSVKRRILCLAREENLASKEVLKT- 350

Query: 427 FQGTTSASRKAELGPVAILFSGGLDSMILAALLDECLDACYEIDLLNVSF---------- 486
                  S KA    +AILFSGG+DSM++AAL D  +     IDLLNV+F          
Sbjct: 351 ------CSSKAN---IAILFSGGVDSMVIAALADRHIPLDEPIDLLNVAFVPKQKTGLPI 410

Query: 487 ----------------------------DGCSAPDRISAKAGVQELRRIAPLRRHYYELY 546
                                            PDR++ KAG++EL+ + P R       
Sbjct: 411 PNIERKQQNHHEIPSEESSQSPAADEGPGEAEVPDRVTGKAGLKELQSVNPSRT------ 470

Query: 547 LGWKLVEVDADLSDLTS-ETKHVISLINPANTYMDLNIGIALWLASGGNGWIYEAIDKDE 606
             W  VE++  L +L       +  L+ P +T +D +IG A+W AS G GW+   + +D 
Sbjct: 471 --WNFVEINVSLEELQKLRRARICHLVQPLDTVLDDSIGCAVWFASRGIGWL---VTQDA 530

Query: 607 KFHRRVKYKSEAKILLVGSGADEQCAGYGRHRTKFRQGGWHLLNEEMKLDIQRIWKRNLG 666
                  YKS AK++L G GADEQ AGY RHR +F+  G   LNEE+ +++ RI  RNLG
Sbjct: 531 V----RSYKSSAKVILTGIGADEQLAGYSRHRARFQSLGLEGLNEEIAMELGRISSRNLG 590

Query: 667 RDDRCIADNGKEARFPFLDEDVINTLLSFPLWEVTDLEKPVGTGDKKILREVAKLLGLHE 671
           RDDR I D+GKEARFPFLDE+V++ L S P+WE  DL  P G G+K ILR  A  LGL  
Sbjct: 591 RDDRVIGDHGKEARFPFLDENVVSFLNSLPVWEKVDLTLPRGVGEKLILRLAAMELGLPA 615

BLAST of MS015239 vs. ExPASy Swiss-Prot
Match: Q4R5D4 (Asparagine synthetase domain-containing protein 1 OS=Macaca fascicularis OX=9541 GN=ASNSD1 PE=2 SV=1)

HSP 1 Score: 294.7 bits (753), Expect = 2.8e-78
Identity = 207/625 (33.12%), Postives = 308/625 (49.28%), Query Frame = 0

Query: 127 GATLQLRGLNPIVQPLMDASRNILVYNGEIFGGIPIERDENDAETLMQALGTCCASNFNL 186
           G  L LRG+    QP+ D   N+ ++NGEIF GI +E +END + L   L +C       
Sbjct: 51  GHVLHLRGV-LTTQPVEDERGNVFLWNGEIFSGIKVEAEENDTQILFNYLSSC------- 110

Query: 187 DISTCNGEGKGQSTIVDILSKIQGPWSIIYWQDSAKTLWFGRDAFGRRSLLVHWPTVEDS 246
                    K +S I+ + S++QGPWS IY+Q S+  LWFGRD FGRRSLL H+  +  S
Sbjct: 111 ---------KNESEILSLFSEVQGPWSFIYYQASSHYLWFGRDFFGRRSLLWHFSNLGKS 170

Query: 247 RFLLSSASPASSIFHSSGLDDDNGTCEVSFWEELPC-GIYSISFDAQSRDCCLVGAVKKH 306
            F LSS         +SGL +         W+E+P  G++ I   + +   C++  ++ +
Sbjct: 171 -FCLSSVGT-----QTSGLANQ--------WQEVPASGLFRIDLKSTAISRCII--LQLY 230

Query: 307 EWTNESLKELVEWKRTRIEPEPGNISASY-----------------LKDFLSQHECNSGC 366
            W   S + ++E     +     ++ A                   L   L Q    + C
Sbjct: 231 PWKYISRENIIEENVNSLSQISADLPAFVSVVANEAKLYLEKPVVPLNMMLPQAALETHC 290

Query: 367 LHNLQSISGS------------IEAPVSTPAEIVLDALRKSL--IRRTSLYTVFQGTTSA 426
             N+ ++  +            I+  +    +++  A++K +  + R    T  + +   
Sbjct: 291 -SNISNVPPTRETLQVFLTDVHIKEVIQQFIDVLSVAVKKRVLCLPRGENLTANEVSKMC 350

Query: 427 SRKAELGPVAILFSGGLDSMILAALLDECLDACYEIDLLNVSF----------------- 486
            RKA    VAILFSGG+DSM++A L D  +     IDLLNV+F                 
Sbjct: 351 DRKAN---VAILFSGGIDSMVIATLADRHIPLDEPIDLLNVAFIAEEKTMPTSFNKERNK 410

Query: 487 --DGC-----------------------SAPDRISAKAGVQELRRIAPLRRHYYELYLGW 546
             + C                       S PDRI+ +AG++EL+ ++P R         W
Sbjct: 411 QKNKCEISSEEFSKDDAAADDDSPDKHVSVPDRITGRAGLKELKAVSPSRI--------W 470

Query: 547 KLVEVDADLSDLTS-ETKHVISLINPANTYMDLNIGIALWLASGGNGWIYEAIDKDEKFH 606
             VE++  + +L       +  LI P +T +D +IG A+W AS G GW+   + +D    
Sbjct: 471 NFVEINVSMEELQKLRRTRICHLIQPLDTVLDDSIGCAVWFASRGIGWL---VAQDGV-- 530

Query: 607 RRVKYKSEAKILLVGSGADEQCAGYGRHRTKFRQGGWHLLNEEMKLDIQRIWKRNLGRDD 666
               Y+S AK++L G GADEQ AGY RHR +F+  G   LN+E+ +++ RI  RNLGRDD
Sbjct: 531 --KSYQSSAKVVLTGIGADEQLAGYSRHRVRFQSHGLEGLNKEIMMELGRISSRNLGRDD 590

Query: 667 RCIADNGKEARFPFLDEDVINTLLSFPLWEVTDLEKPVGTGDKKILREVAKLLGLHEAGA 677
           R I D+GKEARFPFLDE+V++ L S P+WE  +L  P G G+K +LR  A  LGL  +  
Sbjct: 591 RVIGDHGKEARFPFLDENVVSFLNSLPIWEKANLTLPRGIGEKLLLRLAAVELGLTASAL 623

BLAST of MS015239 vs. ExPASy Swiss-Prot
Match: Q9NWL6 (Asparagine synthetase domain-containing protein 1 OS=Homo sapiens OX=9606 GN=ASNSD1 PE=2 SV=2)

HSP 1 Score: 292.0 bits (746), Expect = 1.8e-77
Identity = 205/612 (33.50%), Postives = 302/612 (49.35%), Query Frame = 0

Query: 130 LQLRGLNPIVQPLMDASRNILVYNGEIFGGIPIERDENDAETLMQALGTCCASNFNLDIS 189
           L LRG+    QP+ D   N+ ++NGEIF GI +E +END + L   L +C          
Sbjct: 54  LHLRGV-LTTQPVEDERGNVFLWNGEIFSGIKVEAEENDTQILFNYLSSC---------- 113

Query: 190 TCNGEGKGQSTIVDILSKIQGPWSIIYWQDSAKTLWFGRDAFGRRSLLVHWPTVEDSRFL 249
                 K +S I+ + S++QGPWS IY+Q S+  LWFGRD FGRRSLL H+  +  S F 
Sbjct: 114 ------KNESEILSLFSEVQGPWSFIYYQASSHYLWFGRDFFGRRSLLWHFSNLGKS-FC 173

Query: 250 LSSASPASSIFHSSGLDDDNGTCEVSFWEELPC-GIYSISFDAQSRDCCLVGAVKKHEWT 309
           LSS         +SGL +         W+E+P  G++ I   +     C++  ++ + W 
Sbjct: 174 LSSVGT-----QTSGLANQ--------WQEVPASGLFRIDLKSTVISGCII--LQLYPWK 233

Query: 310 NESLKELVEWKRTRIEPEPGNISASY-----------------LKDFLSQHECNSGCLHN 369
             S + ++E     +     ++ A                   L   L Q    + C  N
Sbjct: 234 YISRENIIEENVNSLSQISADLPAFVSVVANEAKLYLEKPVVPLNMMLPQAALETHC-SN 293

Query: 370 LQSISGS------------IEAPVSTPAEIVLDALRKSL--IRRTSLYTVFQGTTSASRK 429
           + ++  +            ++  +    +++  A++K +  + R    T  +   +  RK
Sbjct: 294 ISNVPPTREILQVFLTDVHMKEVIQQFIDVLSVAVKKRVLCLPRDENLTANEVLKTCDRK 353

Query: 430 AELGPVAILFSGGLDSMILAALLDECLDACYEIDLLNVSF-------------------D 489
           A    VAILFSGG+DSM++A L D  +     IDLLNV+F                   +
Sbjct: 354 AN---VAILFSGGIDSMVIATLADRHIPLDEPIDLLNVAFIAEEKTMPTTFNREGNKQKN 413

Query: 490 GC-----------------------SAPDRISAKAGVQELRRIAPLRRHYYELYLGWKLV 549
            C                       S PDRI+ +AG++EL+ ++P R         W  V
Sbjct: 414 KCEIPSEEFSKDVAAAAADSPNKHVSVPDRITGRAGLKELQAVSPSRI--------WNFV 473

Query: 550 EVDADLSDLTS-ETKHVISLINPANTYMDLNIGIALWLASGGNGWIYEAIDKDEKFHRRV 609
           E++  + +L       +  LI P +T +D +IG A+W AS G GW+             V
Sbjct: 474 EINVSMEELQKLRRTRICHLIRPLDTVLDDSIGCAVWFASRGIGWLVA--------QEGV 533

Query: 610 K-YKSEAKILLVGSGADEQCAGYGRHRTKFRQGGWHLLNEEMKLDIQRIWKRNLGRDDRC 666
           K Y+S AK++L G GADEQ AGY RHR +F+  G   LN+E+ +++ RI  RNLGRDDR 
Sbjct: 534 KSYQSNAKVVLTGIGADEQLAGYSRHRVRFQSHGLEGLNKEIMMELGRISSRNLGRDDRV 593

BLAST of MS015239 vs. ExPASy TrEMBL
Match: A0A6J1D6R1 (asparagine synthetase domain-containing protein 1 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111017558 PE=4 SV=1)

HSP 1 Score: 1369.8 bits (3544), Expect = 0.0e+00
Identity = 681/694 (98.13%), Postives = 682/694 (98.27%), Query Frame = 0

Query: 1   MCGIALIFSGVRLNLSLLHVDARSPSLHPVHEQLEFPIDGLKAALRRRGPDSLGGVKVFI 60
           MCGIALIFSGVRLNLSLLHVDARSPSLHPVHEQLEFPIDGLKAALRRRGPDSLGGVKVFI
Sbjct: 1   MCGIALIFSGVRLNLSLLHVDARSPSLHPVHEQLEFPIDGLKAALRRRGPDSLGGVKVFI 60

Query: 61  SSKSSSHGEENLFECFIERVDDGDAFENQNDRYKMENSCFCSENGLVLQSENSHSDPTSV 120
           SSKSSSHGEENLFECFIERVDDGDAFENQNDRYKMENSCFCSENGLVLQSENSHSDPTSV
Sbjct: 61  SSKSSSHGEENLFECFIERVDDGDAFENQNDRYKMENSCFCSENGLVLQSENSHSDPTSV 120

Query: 121 AELYLIGATLQLRGLNPIVQPLMDASRNILVYNGEIFGGIPIERDENDAETLMQALGTCC 180
           AELYLIGATLQLRGLNPIVQPLMDASRNILVYNGEIFGGIPIERDENDAETLMQALGTCC
Sbjct: 121 AELYLIGATLQLRGLNPIVQPLMDASRNILVYNGEIFGGIPIERDENDAETLMQALGTCC 180

Query: 181 ASNFNLDISTCNGEGKGQSTIVDILSKIQGPWSIIYWQDSAKTLWFGRDAFGRRSLLVHW 240
           ASNFNLDISTCNGEGKGQSTIVDILSKIQGPWSIIYWQD AKTLWFGRDAFGRRSLLVHW
Sbjct: 181 ASNFNLDISTCNGEGKGQSTIVDILSKIQGPWSIIYWQDRAKTLWFGRDAFGRRSLLVHW 240

Query: 241 PTVEDSRFLLSSASPASSIFHSSGLDDDNGTCEVSFWEELPCGIYSISFDAQSRDCCLVG 300
           PTVEDSRFLLSS SPASSIFHSSGLDDDNGTCEVSFWEELPCGIYSISFDAQSRDCCLVG
Sbjct: 241 PTVEDSRFLLSSVSPASSIFHSSGLDDDNGTCEVSFWEELPCGIYSISFDAQSRDCCLVG 300

Query: 301 AVKKHEWTNESLKELVEWKRTRIEPEPGNISASYLKDFLSQHECNSGCLHNLQSISGSIE 360
           AVKKHEWTNESLKELVEWKRTRIEPEPGNISASYLKDFLSQHECNSGCLHNLQSISGS E
Sbjct: 301 AVKKHEWTNESLKELVEWKRTRIEPEPGNISASYLKDFLSQHECNSGCLHNLQSISGSTE 360

Query: 361 APVSTPAEIVLDALRKSLIRRTSLYTVFQGTTSASRKAELGPVAILFSGGLDSMILAALL 420
           APVSTPAEIVLDALRKSLIRRTSLYTVFQGTTSASRKAELGPVAILFSGGLDSMILAALL
Sbjct: 361 APVSTPAEIVLDALRKSLIRRTSLYTVFQGTTSASRKAELGPVAILFSGGLDSMILAALL 420

Query: 421 DECLDACYEIDLLNVSFDGCSAPDRISAKAGVQELRRIAPLRRHYYELYLGWKLVEVDAD 480
           DECLDACYEIDLLNVSFDGCSAPDRISAKAGVQELRRIAPLRR        WKLVEVDAD
Sbjct: 421 DECLDACYEIDLLNVSFDGCSAPDRISAKAGVQELRRIAPLRR--------WKLVEVDAD 480

Query: 481 LSDLTSETKHVISLINPANTYMDLNIGIALWLASGGNGWIYEAIDKDEKFHRRVKYKSEA 540
           LSDLTSETKHV+SLINPANTYMDLNIGIALWLASGGNGWIYEAIDKDEKFHRRVKYKSEA
Sbjct: 481 LSDLTSETKHVMSLINPANTYMDLNIGIALWLASGGNGWIYEAIDKDEKFHRRVKYKSEA 540

Query: 541 KILLVGSGADEQCAGYGRHRTKFRQGGWHLLNEEMKLDIQRIWKRNLGRDDRCIADNGKE 600
           KILLVGSGADEQCAGYGRHRTKFRQGGWHLLNEEMKLDIQRIWKRNLGRDDRCIADNGKE
Sbjct: 541 KILLVGSGADEQCAGYGRHRTKFRQGGWHLLNEEMKLDIQRIWKRNLGRDDRCIADNGKE 600

Query: 601 ARFPFLDEDVINTLLSFPLWEVTDLEKPVGTGDKKILREVAKLLGLHEAGALAKRAIQFG 660
           ARFPFLDEDVINTLLSFPLWEVTDLEKPVGTGDKKILREVAKLLGLHEAGALAKRAIQFG
Sbjct: 601 ARFPFLDEDVINTLLSFPLWEVTDLEKPVGTGDKKILREVAKLLGLHEAGALAKRAIQFG 660

Query: 661 SRIARESNRKNFGSNRAANQASAGSAKIKQHTSS 695
           SRIARESNRKNFGSNRAANQASAGS KIKQHTSS
Sbjct: 661 SRIARESNRKNFGSNRAANQASAGSVKIKQHTSS 686

BLAST of MS015239 vs. ExPASy TrEMBL
Match: A0A6J1D764 (asparagine synthetase domain-containing protein 1 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111017558 PE=4 SV=1)

HSP 1 Score: 1310.4 bits (3390), Expect = 0.0e+00
Identity = 647/660 (98.03%), Postives = 648/660 (98.18%), Query Frame = 0

Query: 1   MCGIALIFSGVRLNLSLLHVDARSPSLHPVHEQLEFPIDGLKAALRRRGPDSLGGVKVFI 60
           MCGIALIFSGVRLNLSLLHVDARSPSLHPVHEQLEFPIDGLKAALRRRGPDSLGGVKVFI
Sbjct: 1   MCGIALIFSGVRLNLSLLHVDARSPSLHPVHEQLEFPIDGLKAALRRRGPDSLGGVKVFI 60

Query: 61  SSKSSSHGEENLFECFIERVDDGDAFENQNDRYKMENSCFCSENGLVLQSENSHSDPTSV 120
           SSKSSSHGEENLFECFIERVDDGDAFENQNDRYKMENSCFCSENGLVLQSENSHSDPTSV
Sbjct: 61  SSKSSSHGEENLFECFIERVDDGDAFENQNDRYKMENSCFCSENGLVLQSENSHSDPTSV 120

Query: 121 AELYLIGATLQLRGLNPIVQPLMDASRNILVYNGEIFGGIPIERDENDAETLMQALGTCC 180
           AELYLIGATLQLRGLNPIVQPLMDASRNILVYNGEIFGGIPIERDENDAETLMQALGTCC
Sbjct: 121 AELYLIGATLQLRGLNPIVQPLMDASRNILVYNGEIFGGIPIERDENDAETLMQALGTCC 180

Query: 181 ASNFNLDISTCNGEGKGQSTIVDILSKIQGPWSIIYWQDSAKTLWFGRDAFGRRSLLVHW 240
           ASNFNLDISTCNGEGKGQSTIVDILSKIQGPWSIIYWQD AKTLWFGRDAFGRRSLLVHW
Sbjct: 181 ASNFNLDISTCNGEGKGQSTIVDILSKIQGPWSIIYWQDRAKTLWFGRDAFGRRSLLVHW 240

Query: 241 PTVEDSRFLLSSASPASSIFHSSGLDDDNGTCEVSFWEELPCGIYSISFDAQSRDCCLVG 300
           PTVEDSRFLLSS SPASSIFHSSGLDDDNGTCEVSFWEELPCGIYSISFDAQSRDCCLVG
Sbjct: 241 PTVEDSRFLLSSVSPASSIFHSSGLDDDNGTCEVSFWEELPCGIYSISFDAQSRDCCLVG 300

Query: 301 AVKKHEWTNESLKELVEWKRTRIEPEPGNISASYLKDFLSQHECNSGCLHNLQSISGSIE 360
           AVKKHEWTNESLKELVEWKRTRIEPEPGNISASYLKDFLSQHECNSGCLHNLQSISGS E
Sbjct: 301 AVKKHEWTNESLKELVEWKRTRIEPEPGNISASYLKDFLSQHECNSGCLHNLQSISGSTE 360

Query: 361 APVSTPAEIVLDALRKSLIRRTSLYTVFQGTTSASRKAELGPVAILFSGGLDSMILAALL 420
           APVSTPAEIVLDALRKSLIRRTSLYTVFQGTTSASRKAELGPVAILFSGGLDSMILAALL
Sbjct: 361 APVSTPAEIVLDALRKSLIRRTSLYTVFQGTTSASRKAELGPVAILFSGGLDSMILAALL 420

Query: 421 DECLDACYEIDLLNVSFDGCSAPDRISAKAGVQELRRIAPLRRHYYELYLGWKLVEVDAD 480
           DECLDACYEIDLLNVSFDGCSAPDRISAKAGVQELRRIAPLRR        WKLVEVDAD
Sbjct: 421 DECLDACYEIDLLNVSFDGCSAPDRISAKAGVQELRRIAPLRR--------WKLVEVDAD 480

Query: 481 LSDLTSETKHVISLINPANTYMDLNIGIALWLASGGNGWIYEAIDKDEKFHRRVKYKSEA 540
           LSDLTSETKHV+SLINPANTYMDLNIGIALWLASGGNGWIYEAIDKDEKFHRRVKYKSEA
Sbjct: 481 LSDLTSETKHVMSLINPANTYMDLNIGIALWLASGGNGWIYEAIDKDEKFHRRVKYKSEA 540

Query: 541 KILLVGSGADEQCAGYGRHRTKFRQGGWHLLNEEMKLDIQRIWKRNLGRDDRCIADNGKE 600
           KILLVGSGADEQCAGYGRHRTKFRQGGWHLLNEEMKLDIQRIWKRNLGRDDRCIADNGKE
Sbjct: 541 KILLVGSGADEQCAGYGRHRTKFRQGGWHLLNEEMKLDIQRIWKRNLGRDDRCIADNGKE 600

Query: 601 ARFPFLDEDVINTLLSFPLWEVTDLEKPVGTGDKKILREVAKLLGLHEAGALAKRAIQFG 660
           ARFPFLDEDVINTLLSFPLWEVTDLEKPVGTGDKKILREVAKLLGLHEAGALAKRAIQ G
Sbjct: 601 ARFPFLDEDVINTLLSFPLWEVTDLEKPVGTGDKKILREVAKLLGLHEAGALAKRAIQGG 652

BLAST of MS015239 vs. ExPASy TrEMBL
Match: A0A5A7SX84 (Asparagine synthetase domain-containing protein 1 isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G004870 PE=4 SV=1)

HSP 1 Score: 1157.9 bits (2994), Expect = 0.0e+00
Identity = 572/693 (82.54%), Postives = 619/693 (89.32%), Query Frame = 0

Query: 1   MCGIALIFSGVRLNLSLLHVDARSPSLHPVHEQLEFPIDGLKAALRRRGPDSLGGVKVFI 60
           MCGIALI SG+RL+LS LHVDAR  S   VHEQLEF ID LKA LRRRGPDSLGGVKV +
Sbjct: 1   MCGIALIVSGIRLDLSSLHVDARPLSPSSVHEQLEFSIDDLKAVLRRRGPDSLGGVKVSL 60

Query: 61  SSKSSSHGEENLFECFIERVDDGDAFENQNDRYKMENSCFCSENGLVLQSENSHSDPTSV 120
           SSKSS   EE   ECFIERVDD  AF+NQND+Y+MEN  FCSENG +LQSEN  S+P S 
Sbjct: 61  SSKSSYPSEEQQLECFIERVDDCVAFQNQNDQYEMENGRFCSENGCILQSENGCSNPISG 120

Query: 121 AELYLIGATLQLRGLNPIVQPLMDASRNILVYNGEIFGGIPIERDENDAETLMQALGTCC 180
             LYL+GATLQLRG+NPIVQPLMDASRNILVYNGEIFGG+ +E  ENDAE+LM AL  CC
Sbjct: 121 TGLYLLGATLQLRGVNPIVQPLMDASRNILVYNGEIFGGLHVECHENDAESLMLALRNCC 180

Query: 181 ASNFNLDISTCNGEGKGQSTIVDILSKIQGPWSIIYWQDSAKTLWFGRDAFGRRSLLVHW 240
           + NF+LD++ C  E K + TIVD LSKIQGPWSIIYWQDSAKTLWFGRDAFGRRSLLVHW
Sbjct: 181 SCNFSLDMNACEDECKRERTIVDTLSKIQGPWSIIYWQDSAKTLWFGRDAFGRRSLLVHW 240

Query: 241 PTVEDSRFLLSSASPASSIFHSSGLDDDNGTCEVSFWEELPCGIYSISFDAQSRDCCLVG 300
           PTVEDSRFLLSS SPASS+F  SGL+ D+GTC+VSFWEELPCGIYS+SFDAQ  D C+VG
Sbjct: 241 PTVEDSRFLLSSVSPASSMFQDSGLEVDSGTCQVSFWEELPCGIYSVSFDAQKTDGCVVG 300

Query: 301 AVKKHEWTNESLKELVEWKRTRIEPEPGNISASYLKDFLSQHECNSGCLHNLQSISGSIE 360
            V++HEWTNESLKELVEWKRTRIEPE G+IS SY KD LSQHE +S CLHNLQS  G+ +
Sbjct: 301 EVRRHEWTNESLKELVEWKRTRIEPEHGDISTSYHKDCLSQHESHSTCLHNLQSALGNFQ 360

Query: 361 APVSTPAEIVLDALRKSLIRRTSLYTVFQGTTSASRKAELGPVAILFSGGLDSMILAALL 420
           APVSTPAEI+LDALRKSL+RRTS YT+FQ TTSASRKAELGPVAILFSGGLDSMILAALL
Sbjct: 361 APVSTPAEILLDALRKSLVRRTSFYTIFQATTSASRKAELGPVAILFSGGLDSMILAALL 420

Query: 421 DECLDACYEIDLLNVSFDGCSAPDRISAKAGVQELRRIAPLRRHY-YELYLGWKLVEVDA 480
           DECLD CYE+DLLNVSFDGCSAPDRISAKAGV+EL RIAPLRRHY  ELYL WKLVE+DA
Sbjct: 421 DECLDPCYEVDLLNVSFDGCSAPDRISAKAGVKELSRIAPLRRHYIIELYLKWKLVEIDA 480

Query: 481 DLSDLTSETKHVISLINPANTYMDLNIGIALWLASGGNGWIYEAIDKDEKFHRRVKYKSE 540
           D+SDLTSETKHV+SLINPANTYMDLNIGIALWLASGGNGWIYE++D+DEKFH+RVKYKSE
Sbjct: 481 DMSDLTSETKHVMSLINPANTYMDLNIGIALWLASGGNGWIYESVDEDEKFHQRVKYKSE 540

Query: 541 AKILLVGSGADEQCAGYGRHRTKFRQGGWHLLNEEMKLDIQRIWKRNLGRDDRCIADNGK 600
           AKILLVGSGADEQCAGYGRHRTK+RQGGWHLLNEEMKLDIQRIWKRNLGRDDRCIADNGK
Sbjct: 541 AKILLVGSGADEQCAGYGRHRTKYRQGGWHLLNEEMKLDIQRIWKRNLGRDDRCIADNGK 600

Query: 601 EARFPFLDEDVINTLLSFPLWEVTDLEKPVGTGDKKILREVAKLLGLHEAGALAKRAIQF 660
           EARFPFLDEDVI TLL+FPLWEVTDLEKP GTGDKKILREVAKLLGLHEAG L KRAIQF
Sbjct: 601 EARFPFLDEDVIRTLLAFPLWEVTDLEKPAGTGDKKILREVAKLLGLHEAGVLPKRAIQF 660

Query: 661 GSRIARESNRKNFGSNRAANQASAGSAKIKQHT 693
           GSRIARESNRKNFGSNRAANQA+AGS K  + +
Sbjct: 661 GSRIARESNRKNFGSNRAANQANAGSVKFNKQS 693

BLAST of MS015239 vs. ExPASy TrEMBL
Match: A0A6J1GYL4 (asparagine synthetase domain-containing protein 1 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111458685 PE=4 SV=1)

HSP 1 Score: 1147.1 bits (2966), Expect = 0.0e+00
Identity = 572/692 (82.66%), Postives = 619/692 (89.45%), Query Frame = 0

Query: 1   MCGIALIFSGVRLNLSLLHVDARSPSLHPVHEQLEFPIDGLKAALRRRGPDSLGGVKVFI 60
           MCGIAL+ SG RL+LS LHVDAR+PS HPVHE LEF ID LKAALRRRGPDSLG VKVFI
Sbjct: 1   MCGIALVVSGCRLDLSSLHVDARAPSPHPVHEHLEFSIDDLKAALRRRGPDSLGEVKVFI 60

Query: 61  SSKSSSHGEENLFECFIERVDDGDAFENQNDRYKMENSCFCSENGLVLQSENSHSDPTSV 120
           SS  S+ GEE  FECF+ERVD G AF+NQND+++M        NG +LQS  S S P S 
Sbjct: 61  SSICSNPGEEQQFECFVERVDCGVAFQNQNDQHEM-------ANGRILQSHASCSSPISG 120

Query: 121 AELYLIGATLQLRGLNPIVQPLMDASRNILVYNGEIFGGIPIERDENDAETLMQALGTCC 180
           A+   +GATLQLRGLNPIVQPLMDASRN+LVYNGEIFGGI +E  +NDAE LMQALG CC
Sbjct: 121 AQFCFLGATLQLRGLNPIVQPLMDASRNVLVYNGEIFGGIHVEYHQNDAEALMQALGNCC 180

Query: 181 ASNFNLDISTCNGEGKGQSTIVDILSKIQGPWSIIYWQDSAKTLWFGRDAFGRRSLLVHW 240
           ASN +LD +TC GE K +STIVD+LS IQGPW+IIY+ DSAKTLWFGRDAFGRRSLLVHW
Sbjct: 181 ASNVSLDTNTCVGECKRESTIVDVLSNIQGPWAIIYFADSAKTLWFGRDAFGRRSLLVHW 240

Query: 241 PTVEDSRFLLSSASPASSIFHSSGLDDDNGTCEVSFWEELPCGIYSISFDAQSRDCCLVG 300
           PTVEDSRFLLSS SPASS+FH SG D+DNGTCEVSFWEELPCGIYSISFDA+S D CLVG
Sbjct: 241 PTVEDSRFLLSSVSPASSMFHDSGHDNDNGTCEVSFWEELPCGIYSISFDARSTDGCLVG 300

Query: 301 AVKKHEWTNESLKELVEWKRTRIEPEPGNISASYLKDFLSQHECNSGCLHNLQSISGSIE 360
            VK+HEWTNESLKEL+EWKRT I P+PG+IS+SY +D LSQHE NS CLHNLQS+S  I+
Sbjct: 301 EVKRHEWTNESLKELIEWKRTCIVPKPGDISSSYHEDLLSQHESNSACLHNLQSVSEGIQ 360

Query: 361 APVSTPAEIVLDALRKSLIRRTSLYTVFQGTTSASRKAELGPVAILFSGGLDSMILAALL 420
           APVSTPAEI+LDALRKSLIRRTSLYT+FQGTTSASRKAELGPVAILFSGGLDSMILAALL
Sbjct: 361 APVSTPAEILLDALRKSLIRRTSLYTIFQGTTSASRKAELGPVAILFSGGLDSMILAALL 420

Query: 421 DECLDACYEIDLLNVSFDGCSAPDRISAKAGVQELRRIAPLRRHYYELYLGWKLVEVDAD 480
           DECLDACYEIDLLNVSFDGCSAPDRISAKAGVQEL RIAPLRR        WKLVE+DAD
Sbjct: 421 DECLDACYEIDLLNVSFDGCSAPDRISAKAGVQELTRIAPLRR--------WKLVEIDAD 480

Query: 481 LSDLTSETKHVISLINPANTYMDLNIGIALWLASGGNGWIYEAIDKDEKFHRRVKYKSEA 540
           LS+LTSETKHV+SLINPANTYMDLNIGIALWLASGG+GWIYE+IDKD+KFHRRVKYKS+A
Sbjct: 481 LSNLTSETKHVMSLINPANTYMDLNIGIALWLASGGDGWIYESIDKDDKFHRRVKYKSQA 540

Query: 541 KILLVGSGADEQCAGYGRHRTKFRQGGWHLLNEEMKLDIQRIWKRNLGRDDRCIADNGKE 600
           KILLVGSGADEQCAGYGRHRTK+RQGGWHLLNEEMKLDIQRIWKRNLGRDDRCIADNGKE
Sbjct: 541 KILLVGSGADEQCAGYGRHRTKYRQGGWHLLNEEMKLDIQRIWKRNLGRDDRCIADNGKE 600

Query: 601 ARFPFLDEDVINTLLSFPLWEVTDLEKPVGTGDKKILREVAKLLGLHEAGALAKRAIQFG 660
           ARFPFLDEDVI+TLL+FPLWEVT+LEKP GTGDKKILREVAKLLGLHEAGAL KRAIQFG
Sbjct: 601 ARFPFLDEDVIHTLLAFPLWEVTNLEKPAGTGDKKILREVAKLLGLHEAGALPKRAIQFG 660

Query: 661 SRIARESNRKNFGSNRAANQASAGSAKIKQHT 693
           SRIARESNRKNFGSNRAANQASAGS K  +H+
Sbjct: 661 SRIARESNRKNFGSNRAANQASAGSVKFNKHS 677

BLAST of MS015239 vs. ExPASy TrEMBL
Match: A0A6J1IPR8 (asparagine synthetase domain-containing protein 1 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111478341 PE=4 SV=1)

HSP 1 Score: 1144.8 bits (2960), Expect = 0.0e+00
Identity = 572/692 (82.66%), Postives = 617/692 (89.16%), Query Frame = 0

Query: 1   MCGIALIFSGVRLNLSLLHVDARSPSLHPVHEQLEFPIDGLKAALRRRGPDSLGGVKVFI 60
           MCGIAL+ SG RL+LS LHVDAR+PS HPVHE LEF ID L+AALRRRGPDSLGGVKVFI
Sbjct: 1   MCGIALVVSGCRLDLSSLHVDARAPSPHPVHEHLEFSIDDLEAALRRRGPDSLGGVKVFI 60

Query: 61  SSKSSSHGEENLFECFIERVDDGDAFENQNDRYKMENSCFCSENGLVLQSENSHSDPTSV 120
           SS SS+ GEE  FECF+ERVD G AF+NQND+++M       ENG +L S +S S   S 
Sbjct: 61  SSISSNPGEEQQFECFVERVDCGVAFQNQNDQHEM-------ENGRILHSHDSCSSLISG 120

Query: 121 AELYLIGATLQLRGLNPIVQPLMDASRNILVYNGEIFGGIPIERDENDAETLMQALGTCC 180
           A+ Y +GATLQLRGLNPIVQPLMDASRN+LVYNGEIFGGI +E  +NDAE LMQALG CC
Sbjct: 121 AQFYFLGATLQLRGLNPIVQPLMDASRNVLVYNGEIFGGIHVEYHQNDAEALMQALGNCC 180

Query: 181 ASNFNLDISTCNGEGKGQSTIVDILSKIQGPWSIIYWQDSAKTLWFGRDAFGRRSLLVHW 240
           ASN +LD +TC GE K +STIVD+LS IQGPW+IIY+  SAKTLWFGRDAFGRRSLLVHW
Sbjct: 181 ASNVSLDTNTCLGECKRESTIVDVLSNIQGPWAIIYFAVSAKTLWFGRDAFGRRSLLVHW 240

Query: 241 PTVEDSRFLLSSASPASSIFHSSGLDDDNGTCEVSFWEELPCGIYSISFDAQSRDCCLVG 300
           PTV DSRFLLSS SPASS+FH SG D+DNGTCEVSFWEELPCGIYSISFDAQS D CLVG
Sbjct: 241 PTVVDSRFLLSSVSPASSMFHDSGHDNDNGTCEVSFWEELPCGIYSISFDAQSTDGCLVG 300

Query: 301 AVKKHEWTNESLKELVEWKRTRIEPEPGNISASYLKDFLSQHECNSGCLHNLQSISGSIE 360
            VK+HEWTNESLKELVEWKRT I P+PG+IS+SY +D LSQHE NS CLHNLQS+   I 
Sbjct: 301 EVKRHEWTNESLKELVEWKRTCIVPKPGDISSSYHEDLLSQHESNSACLHNLQSVPEGIR 360

Query: 361 APVSTPAEIVLDALRKSLIRRTSLYTVFQGTTSASRKAELGPVAILFSGGLDSMILAALL 420
           APVSTPAEI+LDALRKSLIRRTSLYT+FQGTTSASRKAELGPVAILFSGGLDSM LAALL
Sbjct: 361 APVSTPAEILLDALRKSLIRRTSLYTIFQGTTSASRKAELGPVAILFSGGLDSMTLAALL 420

Query: 421 DECLDACYEIDLLNVSFDGCSAPDRISAKAGVQELRRIAPLRRHYYELYLGWKLVEVDAD 480
           DECLDACYEIDLLNVSFDGCSAPDRISAKAGVQEL RIAPLRR        WKLVE+DAD
Sbjct: 421 DECLDACYEIDLLNVSFDGCSAPDRISAKAGVQELTRIAPLRR--------WKLVEIDAD 480

Query: 481 LSDLTSETKHVISLINPANTYMDLNIGIALWLASGGNGWIYEAIDKDEKFHRRVKYKSEA 540
           LS+LTSETKHV+SLINPANTYMDLNIGIALWLASGG+GWIYE+IDKD+KFHRRVKYKS+A
Sbjct: 481 LSNLTSETKHVMSLINPANTYMDLNIGIALWLASGGDGWIYESIDKDDKFHRRVKYKSQA 540

Query: 541 KILLVGSGADEQCAGYGRHRTKFRQGGWHLLNEEMKLDIQRIWKRNLGRDDRCIADNGKE 600
           KILLVGSGADEQCAGYGRHRTK+RQGGWHLLNEEMKLDIQRIWKRNLGRDDRCIADNGKE
Sbjct: 541 KILLVGSGADEQCAGYGRHRTKYRQGGWHLLNEEMKLDIQRIWKRNLGRDDRCIADNGKE 600

Query: 601 ARFPFLDEDVINTLLSFPLWEVTDLEKPVGTGDKKILREVAKLLGLHEAGALAKRAIQFG 660
           ARFPFLDEDVI+TLL+FPLWEVTDLEKP GTGDKKILREVAKLLGLHEAGAL KRAIQFG
Sbjct: 601 ARFPFLDEDVIHTLLAFPLWEVTDLEKPAGTGDKKILREVAKLLGLHEAGALPKRAIQFG 660

Query: 661 SRIARESNRKNFGSNRAANQASAGSAKIKQHT 693
           SRIARESNRKNFGSNRAANQASAGS K  +H+
Sbjct: 661 SRIARESNRKNFGSNRAANQASAGSVKFNKHS 677

BLAST of MS015239 vs. TAIR 10
Match: AT2G03667.1 (Asparagine synthase family protein )

HSP 1 Score: 695.3 bits (1793), Expect = 5.1e-200
Identity = 379/689 (55.01%), Postives = 471/689 (68.36%), Query Frame = 0

Query: 1   MCGIALIFSGVRLNLSLLHVDARSPSLHPVHEQLEFPIDGLKAALRRRGPDSLGGVKVFI 60
           MCGIA++  GVR+ LS L   +         E+L+  ++ +KA L +RGPDS+G   + +
Sbjct: 1   MCGIAIVVCGVRIELSTL--SSSLSETETPFERLQVSVEDVKAVLSQRGPDSVGEKTIHL 60

Query: 61  SSKSSSHGEENLFECFIERVDDGDAFENQNDRYKMENSCFCSENGLVLQSENSHSDPTSV 120
                  G+E+     +         E     YK+E                   + TS+
Sbjct: 61  RPNC---GQESATLSVV---------ETSEGTYKLE-------------------ETTSL 120

Query: 121 AELYLIGATLQLRGLNPIVQPLMDASRNILVYNGEIFGGIPIERDENDAETLMQALGTCC 180
            EL+ IG+TLQLRG +PI QPL+D+S NIL YNGE+FGGI +   +ND E L+++L    
Sbjct: 121 GELHFIGSTLQLRGTSPIRQPLVDSSGNILAYNGEVFGGIELNSYDNDTEVLLKSL---- 180

Query: 181 ASNFNLDISTCNGEGKGQSTIVDILSKIQGPWSIIYWQDSAKTLWFGRDAFGRRSLLVHW 240
                          K +S + D+LS I+GPW+IIYWQ+S++TLWFG+D FGRRSLLVH 
Sbjct: 181 --------------EKAKSLVPDVLSMIKGPWAIIYWQESSRTLWFGKDPFGRRSLLVHS 240

Query: 241 PTVEDSRFLLSSASPASSIFHSSGLDDDNGTCEVSFWEELPCGIYSISFDAQSRDCCLVG 300
           PTVED RFLLSS SPASS+  S GLD +NG     +WEELPCG+YSISF     + C+ G
Sbjct: 241 PTVEDPRFLLSSVSPASSV--SQGLDTENGNSLHRYWEELPCGVYSISFGVS--ELCIHG 300

Query: 301 AVKKHEWTNESLKELVEWKRTRIEPEPGNISASYLKDFLSQHECNSGCLHNLQSISGSIE 360
            V KHEW N   KEL+EW+R  + P P ++S S L          SG   + +S+S S+ 
Sbjct: 301 EVTKHEWRNTIWKELIEWERKLVVPRPEDLSTSSL----------SGVQED-KSVSTSLG 360

Query: 361 APVSTPAEIVLDALRKSLIRRTSLYTVFQGTTSASRKAELGPVAILFSGGLDSMILAALL 420
                 A+ VL  L++S+ RRTSL++++QG   A       PVA+LFSGGLDSMILAALL
Sbjct: 361 F-----AQTVLVVLKESVRRRTSLHSIYQGDKEAV------PVAVLFSGGLDSMILAALL 420

Query: 421 DECLDACYEIDLLNVSFDGCSAPDRISAKAGVQELRRIAPLRRHYYELYLGWKLVEVDAD 480
            +CLD  YE+DLLNVSFDG +APDRISAKAG++EL++IAP RR        WKLVE+DAD
Sbjct: 421 HQCLDPKYEVDLLNVSFDGPNAPDRISAKAGIKELKKIAPFRR--------WKLVEIDAD 480

Query: 481 LSDLTSETKHVISLINPANTYMDLNIGIALWLASGGNGWIYE--AIDKDEKFHRRVKYKS 540
           LS L  ETK V+SLINPA+TYMDLNIG ALWLA+ G+GWI+E       E+ ++RVKYKS
Sbjct: 481 LSKLGFETKRVMSLINPADTYMDLNIGTALWLAARGDGWIHEDNGNPSVEENNQRVKYKS 540

Query: 541 EAKILLVGSGADEQCAGYGRHRTKFRQGGWHLLNEEMKLDIQRIWKRNLGRDDRCIADNG 600
           +A+ILLVG+GADEQCAGYGRHRTK+R G W  L++EMKLD+QRIWKRNLGRDDRCIADNG
Sbjct: 541 DARILLVGAGADEQCAGYGRHRTKYRNGSWVALDQEMKLDMQRIWKRNLGRDDRCIADNG 600

Query: 601 KEARFPFLDEDVINTLLSFPLWEVTDLEKPVGTGDKKILREVAKLLGLHEAGALAKRAIQ 660
           KE RFPFLDEDVI TLL  PLWE+ DLE+P GTGDKKILR+VA LLG+HE   + KRAIQ
Sbjct: 601 KEGRFPFLDEDVIKTLLDIPLWEIADLEQPSGTGDKKILRQVASLLGVHEVAKMPKRAIQ 604

Query: 661 FGSRIARESNRKNFGSNRAANQASAGSAK 688
           FGSRIARESNRKNFGSNRAANQASAGS +
Sbjct: 661 FGSRIARESNRKNFGSNRAANQASAGSVR 604

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022149042.10.0e+0098.13asparagine synthetase domain-containing protein 1 isoform X2 [Momordica charanti... [more]
XP_022149041.10.0e+0098.03asparagine synthetase domain-containing protein 1 isoform X1 [Momordica charanti... [more]
XP_038892575.10.0e+0083.53asparagine synthetase domain-containing protein 1 [Benincasa hispida][more]
KAA0034476.10.0e+0082.54asparagine synthetase domain-containing protein 1 isoform X2 [Cucumis melo var. ... [more]
XP_022957237.10.0e+0082.66asparagine synthetase domain-containing protein 1 isoform X1 [Cucurbita moschata... [more]
Match NameE-valueIdentityDescription
Q8JFW13.0e-8034.49Asparagine synthetase domain-containing protein 1 OS=Danio rerio OX=7955 GN=asns... [more]
Q0V8E41.1e-7934.56Asparagine synthetase domain-containing protein 1 OS=Bos taurus OX=9913 GN=ASNSD... [more]
Q8BFS99.6e-7934.29Asparagine synthetase domain-containing protein 1 OS=Mus musculus OX=10090 GN=As... [more]
Q4R5D42.8e-7833.12Asparagine synthetase domain-containing protein 1 OS=Macaca fascicularis OX=9541... [more]
Q9NWL61.8e-7733.50Asparagine synthetase domain-containing protein 1 OS=Homo sapiens OX=9606 GN=ASN... [more]
Match NameE-valueIdentityDescription
A0A6J1D6R10.0e+0098.13asparagine synthetase domain-containing protein 1 isoform X2 OS=Momordica charan... [more]
A0A6J1D7640.0e+0098.03asparagine synthetase domain-containing protein 1 isoform X1 OS=Momordica charan... [more]
A0A5A7SX840.0e+0082.54Asparagine synthetase domain-containing protein 1 isoform X2 OS=Cucumis melo var... [more]
A0A6J1GYL40.0e+0082.66asparagine synthetase domain-containing protein 1 isoform X1 OS=Cucurbita moscha... [more]
A0A6J1IPR80.0e+0082.66asparagine synthetase domain-containing protein 1 isoform X1 OS=Cucurbita maxima... [more]
Match NameE-valueIdentityDescription
AT2G03667.15.1e-20055.01Asparagine synthase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR017932Glutamine amidotransferase type 2 domainPFAMPF13537GATase_7coord: 140..253
e-value: 4.3E-7
score: 29.9
IPR001962Asparagine synthasePFAMPF00733Asn_synthasecoord: 531..646
e-value: 6.7E-9
score: 35.9
IPR001962Asparagine synthaseCDDcd01991Asn_Synthase_B_Ccoord: 401..660
e-value: 1.08301E-30
score: 119.446
IPR014729Rossmann-like alpha/beta/alpha sandwich foldGENE3D3.40.50.620HUPscoord: 365..693
e-value: 3.2E-58
score: 199.7
IPR029055Nucleophile aminohydrolases, N-terminalGENE3D3.60.20.10Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1coord: 29..264
e-value: 4.6E-16
score: 60.6
IPR029055Nucleophile aminohydrolases, N-terminalSUPERFAMILY56235N-terminal nucleophile aminohydrolases (Ntn hydrolases)coord: 43..255
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 668..694
NoneNo IPR availablePANTHERPTHR45937ASPARAGINE SYNTHETASE DOMAIN-CONTAINING PROTEIN 1coord: 1..680
NoneNo IPR availableSUPERFAMILY52402Adenine nucleotide alpha hydrolases-likecoord: 397..681

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS015239.1MS015239.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006529 asparagine biosynthetic process
molecular_function GO:0004066 asparagine synthase (glutamine-hydrolyzing) activity