MS015213 (gene) Bitter gourd (TR) v1

Overview


Name	MS015213
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Eukaryotic translation initiation factor 5A
Location	scaffold2: 1843790 .. 1845555 (-)
RNA-Seq Expression	MS015213
Synteny	MS015213

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCCGACGAGGAGCATCACTTCGAGTCGAAGGCCGACGCCGGAGCTTCCAAGACTTATCCTCAGCAGGCCGGGACCATCCGTAAGAATGGTTACATTGTCATCAAGGGCAGGCCTTGCAAGGTCTGTTTCGTTTTCGTTCTGATACCTCAATTGTCTCTCATCCTTTGTCCCATCGATTCGCATAATTTTTGTTTGATATGTAGTATCTATATATATTTAGATGTATCGTCTGCTTCAGTTTCTCCTTCTCTTGTTGCGTTACGTCTATATCATTTTTAAGGGCTTTCTTTTTTCCTGTTCTGTTCTGTGACTTTGGATAATTGTGTTTTATTTACTGATTGTTGATATGGGGTGTGCTTTTACACTGTTTCAGTCTCTTTTTTAATCTCTTGAGATGCTGTCGTCCATCGAATTTAGTTTTCTAGATCAATCGTCCGTTTCTTTTGGGTGAAATTGATTGACCCATCTAGTTAGCTAAAATGAAACTAATGAATTGTATGAAGCTACCGTACTTCAGTCCGTTTTTTTCTAGGTATTAGTTTTCTCCGCCGTTTGAGTAGAACATTTATTATCTTGTAATATGTTATTCTATAACCAAACTTGACCTTAGGCTTATGTATTTTCGTTTGTAGGTTGTTGAGGTTTCAACCTCTAAGACTGGAAAGCACGGCCATGCTAAATGTCACTTTGTTGGAATTGACATTTTCACTACTAAAAAGCTGGAAGATATCGTCCCCTCTTCCCACAACTGTGACGTATGAAGATCTTGTTCTACTTTTATACTTTTCTTTGTAGGTTGTAGGTTTGTTTGTGTTAGTTTGTGATTTTTTATTCTTGAACTCTTGAAACACAGGTTCCCCATGTCAGTCGCACTGACTACCAGCTCATTGATATCTCTGAAGACGGATTTGTATGATCAAATTCTCTTTGTTTATTTCTTTGTATCAGTTTCCACTGGTTGGTTGTTCATGTAGAGCACCGTGGCTAACCCTAATTTTAATGAAAACTTACCAGATTAGCCTGCTAACGGACAGTGGTAGCACCAAAGATGACCTGAGGCTTCCAACCGATGAGAGTCTTCTGTCCCAGGTAAGATTTTCTTTTTGGCTGTTAACATTTTCTGTGCTGTTGTGTTTAATTGGCCTTAATGATTGATTGCATCCTGAGATTCCTCTGCTGTTACAGTACCATATTGCCTGTTACAATGAAATTATTTCTTTCTCCACTTCTTAAAGTATATTATCCGGTCCTTGTTATTATGGGACTCCAATTTCCACTCTGGGGTATGGAGTTTTAAGTGTGGATGCCTTTCTGCTTTCTTGTTTTTCCTCCTTTTATTATGCTTATGTTCTTCAGAGATATGGGTTTGAGTTTGTTGCAAAATGTGGATGTGTGTAATGTGTGTGGGAAGGTTTGAATAACACTTTTTTAAATTTATAATTGCTAAAATGTTTTTAAAATTCAGAAATGCATTTCGTCCATGATTAACTAACAAGCGAACCATCTTAGTTGAGATTAAAAATTCGAAGTTTGTCCATTCTAATTGTTTTAGTCTGTAATTGTTTTCTTGTGCATAATACTAATCTATAGCACCAAATTATCTAGGTAGTTGAAAAATAATGTTCTATTGATCTATAAGTTTCTACTGGTACTATGTCTTGCAGATCAAGGATGGATTTAACGAGGGGAAGGATCTGATTGTGACTGTCATGTCTTCAATGGGAGAGGAGCAAATTTGCGCTCTCAAGGACATTGGCCCGAAA

mRNA sequence

ATGTCCGACGAGGAGCATCACTTCGAGTCGAAGGCCGACGCCGGAGCTTCCAAGACTTATCCTCAGCAGGCCGGGACCATCCGTAAGAATGGTTACATTGTCATCAAGGGCAGGCCTTGCAAGGTTGTTGAGGTTTCAACCTCTAAGACTGGAAAGCACGGCCATGCTAAATGTCACTTTGTTGGAATTGACATTTTCACTACTAAAAAGCTGGAAGATATCGTCCCCTCTTCCCACAACTGTGACGTTCCCCATGTCAGTCGCACTGACTACCAGCTCATTGATATCTCTGAAGACGGATTTATTAGCCTGCTAACGGACAGTGGTAGCACCAAAGATGACCTGAGGCTTCCAACCGATGAGAGTCTTCTGTCCCAGATCAAGGATGGATTTAACGAGGGGAAGGATCTGATTGTGACTGTCATGTCTTCAATGGGAGAGGAGCAAATTTGCGCTCTCAAGGACATTGGCCCGAAA

Coding sequence (CDS)

ATGTCCGACGAGGAGCATCACTTCGAGTCGAAGGCCGACGCCGGAGCTTCCAAGACTTATCCTCAGCAGGCCGGGACCATCCGTAAGAATGGTTACATTGTCATCAAGGGCAGGCCTTGCAAGGTTGTTGAGGTTTCAACCTCTAAGACTGGAAAGCACGGCCATGCTAAATGTCACTTTGTTGGAATTGACATTTTCACTACTAAAAAGCTGGAAGATATCGTCCCCTCTTCCCACAACTGTGACGTTCCCCATGTCAGTCGCACTGACTACCAGCTCATTGATATCTCTGAAGACGGATTTATTAGCCTGCTAACGGACAGTGGTAGCACCAAAGATGACCTGAGGCTTCCAACCGATGAGAGTCTTCTGTCCCAGATCAAGGATGGATTTAACGAGGGGAAGGATCTGATTGTGACTGTCATGTCTTCAATGGGAGAGGAGCAAATTTGCGCTCTCAAGGACATTGGCCCGAAA

Protein sequence

MSDEEHHFESKADAGASKTYPQQAGTIRKNGYIVIKGRPCKVVEVSTSKTGKHGHAKCHFVGIDIFTTKKLEDIVPSSHNCDVPHVSRTDYQLIDISEDGFISLLTDSGSTKDDLRLPTDESLLSQIKDGFNEGKDLIVTVMSSMGEEQICALKDIGPK

Homology

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BLAST of MS015213 vs. NCBI nr
Match: XP_022151244.1 (eukaryotic translation initiation factor 5A-4-like [Momordica charantia])

HSP 1 Score: 325.1 bits (832), Expect = 3.4e-85
Identity = 159/159 (100.00%), Postives = 159/159 (100.00%), Query Frame = 0


Query: 1   MSDEEHHFESKADAGASKTYPQQAGTIRKNGYIVIKGRPCKVVEVSTSKTGKHGHAKCHF 60
           MSDEEHHFESKADAGASKTYPQQAGTIRKNGYIVIKGRPCKVVEVSTSKTGKHGHAKCHF
Sbjct: 1   MSDEEHHFESKADAGASKTYPQQAGTIRKNGYIVIKGRPCKVVEVSTSKTGKHGHAKCHF 60

Query: 61  VGIDIFTTKKLEDIVPSSHNCDVPHVSRTDYQLIDISEDGFISLLTDSGSTKDDLRLPTD 120
           VGIDIFTTKKLEDIVPSSHNCDVPHVSRTDYQLIDISEDGFISLLTDSGSTKDDLRLPTD
Sbjct: 61  VGIDIFTTKKLEDIVPSSHNCDVPHVSRTDYQLIDISEDGFISLLTDSGSTKDDLRLPTD 120

Query: 121 ESLLSQIKDGFNEGKDLIVTVMSSMGEEQICALKDIGPK 160
           ESLLSQIKDGFNEGKDLIVTVMSSMGEEQICALKDIGPK
Sbjct: 121 ESLLSQIKDGFNEGKDLIVTVMSSMGEEQICALKDIGPK 159

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BLAST of MS015213 vs. NCBI nr
Match: XP_038893432.1 (eukaryotic translation initiation factor 5A-4 [Benincasa hispida])

HSP 1 Score: 318.9 bits (816), Expect = 2.4e-83
Identity = 156/159 (98.11%), Postives = 157/159 (98.74%), Query Frame = 0


Query: 1   MSDEEHHFESKADAGASKTYPQQAGTIRKNGYIVIKGRPCKVVEVSTSKTGKHGHAKCHF 60
           MSDEEHHFESKADAGASKTYPQQAGTIRKNGYIVIKGRPCKVVEVSTSKTGKHGHAKCHF
Sbjct: 1   MSDEEHHFESKADAGASKTYPQQAGTIRKNGYIVIKGRPCKVVEVSTSKTGKHGHAKCHF 60

Query: 61  VGIDIFTTKKLEDIVPSSHNCDVPHVSRTDYQLIDISEDGFISLLTDSGSTKDDLRLPTD 120
           VGIDIFT KKLEDIVPSSHNCDVPHVSRTDYQLIDISEDGFISLLTDSG+TKDDLRLPTD
Sbjct: 61  VGIDIFTAKKLEDIVPSSHNCDVPHVSRTDYQLIDISEDGFISLLTDSGNTKDDLRLPTD 120

Query: 121 ESLLSQIKDGFNEGKDLIVTVMSSMGEEQICALKDIGPK 160
           ESLLSQIKDGF EGKDLIVTVMSSMGEEQICALKDIGPK
Sbjct: 121 ESLLSQIKDGFAEGKDLIVTVMSSMGEEQICALKDIGPK 159

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BLAST of MS015213 vs. NCBI nr
Match: XP_008446407.1 (PREDICTED: eukaryotic translation initiation factor 5A-2 [Cucumis melo] >KAA0034452.1 eukaryotic translation initiation factor 5A-2 [Cucumis melo var. makuwa] >TYK17682.1 eukaryotic translation initiation factor 5A-2 [Cucumis melo var. makuwa])

HSP 1 Score: 317.8 bits (813), Expect = 5.4e-83
Identity = 155/159 (97.48%), Postives = 157/159 (98.74%), Query Frame = 0


Query: 1   MSDEEHHFESKADAGASKTYPQQAGTIRKNGYIVIKGRPCKVVEVSTSKTGKHGHAKCHF 60
           MSDEEHHFESKADAGASKTYPQQAGTIRKNGYIVIKGRPCKVVEVSTSKTGKHGHAKCHF
Sbjct: 1   MSDEEHHFESKADAGASKTYPQQAGTIRKNGYIVIKGRPCKVVEVSTSKTGKHGHAKCHF 60

Query: 61  VGIDIFTTKKLEDIVPSSHNCDVPHVSRTDYQLIDISEDGFISLLTDSGSTKDDLRLPTD 120
           VGIDIFT KKLEDIVPSSHNCDVPHVSRTDYQLIDISEDGFISLLTDSGSTKDDLRLPTD
Sbjct: 61  VGIDIFTAKKLEDIVPSSHNCDVPHVSRTDYQLIDISEDGFISLLTDSGSTKDDLRLPTD 120

Query: 121 ESLLSQIKDGFNEGKDLIVTVMSSMGEEQICALKDIGPK 160
           ++LLSQIKDGF EGKDLIVTVMSSMGEEQICALKDIGPK
Sbjct: 121 DNLLSQIKDGFAEGKDLIVTVMSSMGEEQICALKDIGPK 159

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BLAST of MS015213 vs. NCBI nr
Match: XP_022957459.1 (eukaryotic translation initiation factor 5A-2-like [Cucurbita moschata] >XP_022989737.1 eukaryotic translation initiation factor 5A-2-like [Cucurbita maxima] >XP_023516204.1 eukaryotic translation initiation factor 5A-2-like [Cucurbita pepo subsp. pepo] >KAG6601458.1 Eukaryotic translation initiation factor 5A-2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 313.2 bits (801), Expect = 1.3e-81
Identity = 152/159 (95.60%), Postives = 156/159 (98.11%), Query Frame = 0


Query: 1   MSDEEHHFESKADAGASKTYPQQAGTIRKNGYIVIKGRPCKVVEVSTSKTGKHGHAKCHF 60
           MSDEEHHFESKADAGASKTYPQQAGTIRKNGYIVIKGRPCKVVEVSTSKTGKHGHAKCHF
Sbjct: 1   MSDEEHHFESKADAGASKTYPQQAGTIRKNGYIVIKGRPCKVVEVSTSKTGKHGHAKCHF 60

Query: 61  VGIDIFTTKKLEDIVPSSHNCDVPHVSRTDYQLIDISEDGFISLLTDSGSTKDDLRLPTD 120
           V IDIFT+KKLEDIVPSSHNCDVPHVSRTDYQLIDISEDGFISLLTDSG+TKDDLRLP D
Sbjct: 61  VAIDIFTSKKLEDIVPSSHNCDVPHVSRTDYQLIDISEDGFISLLTDSGNTKDDLRLPND 120

Query: 121 ESLLSQIKDGFNEGKDLIVTVMSSMGEEQICALKDIGPK 160
           ESL++QIKDGF EGKDLIVTVMSSMGEEQICALKDIGPK
Sbjct: 121 ESLMTQIKDGFAEGKDLIVTVMSSMGEEQICALKDIGPK 159

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BLAST of MS015213 vs. NCBI nr
Match: KAG7032242.1 (Eukaryotic translation initiation factor 5A-2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 313.2 bits (801), Expect = 1.3e-81
Identity = 152/159 (95.60%), Postives = 156/159 (98.11%), Query Frame = 0


Query: 1   MSDEEHHFESKADAGASKTYPQQAGTIRKNGYIVIKGRPCKVVEVSTSKTGKHGHAKCHF 60
           MSDEEHHFESKADAGASKTYPQQAGTIRKNGYIVIKGRPCKVVEVSTSKTGKHGHAKCHF
Sbjct: 438 MSDEEHHFESKADAGASKTYPQQAGTIRKNGYIVIKGRPCKVVEVSTSKTGKHGHAKCHF 497

Query: 61  VGIDIFTTKKLEDIVPSSHNCDVPHVSRTDYQLIDISEDGFISLLTDSGSTKDDLRLPTD 120
           V IDIFT+KKLEDIVPSSHNCDVPHVSRTDYQLIDISEDGFISLLTDSG+TKDDLRLP D
Sbjct: 498 VAIDIFTSKKLEDIVPSSHNCDVPHVSRTDYQLIDISEDGFISLLTDSGNTKDDLRLPND 557

Query: 121 ESLLSQIKDGFNEGKDLIVTVMSSMGEEQICALKDIGPK 160
           ESL++QIKDGF EGKDLIVTVMSSMGEEQICALKDIGPK
Sbjct: 558 ESLMTQIKDGFAEGKDLIVTVMSSMGEEQICALKDIGPK 596

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BLAST of MS015213 vs. ExPASy Swiss-Prot
Match: P24922 (Eukaryotic translation initiation factor 5A-2 OS=Nicotiana plumbaginifolia OX=4092 GN=EIF-5A2 PE=2 SV=1)

HSP 1 Score: 300.1 bits (767), Expect = 1.5e-80
Identity = 143/159 (89.94%), Postives = 155/159 (97.48%), Query Frame = 0


Query: 1   MSDEEHHFESKADAGASKTYPQQAGTIRKNGYIVIKGRPCKVVEVSTSKTGKHGHAKCHF 60
           MSDEEH FESKADAGASKTYPQQAGTIRKNG+IVIKGRPCKVVEVSTSKTGKHGHAKCHF
Sbjct: 1   MSDEEHQFESKADAGASKTYPQQAGTIRKNGHIVIKGRPCKVVEVSTSKTGKHGHAKCHF 60

Query: 61  VGIDIFTTKKLEDIVPSSHNCDVPHVSRTDYQLIDISEDGFISLLTDSGSTKDDLRLPTD 120
           V IDIFT KKLEDIVPSSHNCDVPHV+RTDYQLIDISEDGF+SLLT++G+TKDDLRLPTD
Sbjct: 61  VAIDIFTGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTENGNTKDDLRLPTD 120

Query: 121 ESLLSQIKDGFNEGKDLIVTVMSSMGEEQICALKDIGPK 160
           ++LL+QIKDGF EGKDL+V+VMS+MGEEQICALKDIGPK
Sbjct: 121 DNLLTQIKDGFAEGKDLVVSVMSAMGEEQICALKDIGPK 159

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BLAST of MS015213 vs. ExPASy Swiss-Prot
Match: Q9AXJ4 (Eukaryotic translation initiation factor 5A OS=Manihot esculenta OX=3983 PE=2 SV=2)

HSP 1 Score: 300.1 bits (767), Expect = 1.5e-80
Identity = 142/159 (89.31%), Postives = 155/159 (97.48%), Query Frame = 0


Query: 1   MSDEEHHFESKADAGASKTYPQQAGTIRKNGYIVIKGRPCKVVEVSTSKTGKHGHAKCHF 60
           MSDEEHHFESKADAGASKT+PQQAGTIRKNGYIVIK RPCKV+EVSTSKTGKHGHAKCHF
Sbjct: 1   MSDEEHHFESKADAGASKTFPQQAGTIRKNGYIVIKNRPCKVMEVSTSKTGKHGHAKCHF 60

Query: 61  VGIDIFTTKKLEDIVPSSHNCDVPHVSRTDYQLIDISEDGFISLLTDSGSTKDDLRLPTD 120
           VGIDIF  KKLEDIVPSSHNCDVPHV+RTDYQLIDISEDGF+SLLT++G+TKDDLRLPTD
Sbjct: 61  VGIDIFNGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTETGNTKDDLRLPTD 120

Query: 121 ESLLSQIKDGFNEGKDLIVTVMSSMGEEQICALKDIGPK 160
           E+LLSQIKDGF EGKDL+V+VMS+MGEE+IC+LKDIGPK
Sbjct: 121 ENLLSQIKDGFAEGKDLVVSVMSAMGEERICSLKDIGPK 159

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BLAST of MS015213 vs. ExPASy Swiss-Prot
Match: P56336 (Eukaryotic translation initiation factor 5A-4 OS=Solanum tuberosum OX=4113 GN=EIF5A4 PE=2 SV=1)

HSP 1 Score: 299.3 bits (765), Expect = 2.6e-80
Identity = 145/159 (91.19%), Postives = 154/159 (96.86%), Query Frame = 0


Query: 1   MSDEEHHFESKADAGASKTYPQQAGTIRKNGYIVIKGRPCKVVEVSTSKTGKHGHAKCHF 60
           MSDEEH FESKADAGASKTYPQQAGTIRK+GYIVIKGRPCKVVEVSTSKTGKHGHAKCHF
Sbjct: 1   MSDEEHQFESKADAGASKTYPQQAGTIRKSGYIVIKGRPCKVVEVSTSKTGKHGHAKCHF 60

Query: 61  VGIDIFTTKKLEDIVPSSHNCDVPHVSRTDYQLIDISEDGFISLLTDSGSTKDDLRLPTD 120
           V IDIFT KKLEDIVPSSHNCDVPHV+RTDYQLIDISEDGF+SLLTD+G+TKDDLRLPTD
Sbjct: 61  VAIDIFTGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTDNGNTKDDLRLPTD 120

Query: 121 ESLLSQIKDGFNEGKDLIVTVMSSMGEEQICALKDIGPK 160
           +SLLSQIKDGF EGKDL+V+VMS+MGEEQI ALKDIGPK
Sbjct: 121 DSLLSQIKDGFAEGKDLVVSVMSAMGEEQINALKDIGPK 159

[Close]

BLAST of MS015213 vs. ExPASy Swiss-Prot
Match: Q9AXQ4 (Eukaryotic translation initiation factor 5A-3 OS=Solanum lycopersicum OX=4081 PE=2 SV=1)

HSP 1 Score: 298.5 bits (763), Expect = 4.5e-80
Identity = 145/159 (91.19%), Postives = 153/159 (96.23%), Query Frame = 0


Query: 1   MSDEEHHFESKADAGASKTYPQQAGTIRKNGYIVIKGRPCKVVEVSTSKTGKHGHAKCHF 60
           MSDEEH FESKADAGASKTYPQQAGTIRKNGYIVIKGRPCKVVEVSTSKTGKHGHAKCHF
Sbjct: 1   MSDEEHQFESKADAGASKTYPQQAGTIRKNGYIVIKGRPCKVVEVSTSKTGKHGHAKCHF 60

Query: 61  VGIDIFTTKKLEDIVPSSHNCDVPHVSRTDYQLIDISEDGFISLLTDSGSTKDDLRLPTD 120
           V IDIFT KKLEDIVPSSHNCDVPHV+RTDYQLIDISEDGF+SLLTD+G+TKDDLRLPTD
Sbjct: 61  VAIDIFTGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTDNGNTKDDLRLPTD 120

Query: 121 ESLLSQIKDGFNEGKDLIVTVMSSMGEEQICALKDIGPK 160
           E+LLS IKDGF EGKDL+V+VMS+MGEEQI ALKDIGPK
Sbjct: 121 ENLLSLIKDGFAEGKDLVVSVMSAMGEEQINALKDIGPK 159

[Close]

BLAST of MS015213 vs. ExPASy Swiss-Prot
Match: Q945F4 (Eukaryotic translation initiation factor 5A-2 OS=Medicago sativa OX=3879 PE=2 SV=1)

HSP 1 Score: 298.1 bits (762), Expect = 5.8e-80
Identity = 142/159 (89.31%), Postives = 152/159 (95.60%), Query Frame = 0


Query: 1   MSDEEHHFESKADAGASKTYPQQAGTIRKNGYIVIKGRPCKVVEVSTSKTGKHGHAKCHF 60
           MSDEEHHFE  ADAGASKTYPQQAGTIRKNGYIVIK RPCKVVEVSTSKTGKHGHAKCHF
Sbjct: 1   MSDEEHHFEPAADAGASKTYPQQAGTIRKNGYIVIKSRPCKVVEVSTSKTGKHGHAKCHF 60

Query: 61  VGIDIFTTKKLEDIVPSSHNCDVPHVSRTDYQLIDISEDGFISLLTDSGSTKDDLRLPTD 120
           V IDIF  KKLEDIVPSSHNCDVPHV+RTDYQLIDISEDGF+SLLTD+GSTKDDL+LPTD
Sbjct: 61  VAIDIFNGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTDNGSTKDDLKLPTD 120

Query: 121 ESLLSQIKDGFNEGKDLIVTVMSSMGEEQICALKDIGPK 160
           +SLL+QIKDGF +GKDL+V+VMS+MGEEQICALKDIGPK
Sbjct: 121 DSLLTQIKDGFADGKDLVVSVMSAMGEEQICALKDIGPK 159

[Close]

BLAST of MS015213 vs. ExPASy TrEMBL
Match: A0A6J1DCF3 (Eukaryotic translation initiation factor 5A OS=Momordica charantia OX=3673 GN=LOC111019214 PE=3 SV=1)

HSP 1 Score: 325.1 bits (832), Expect = 1.6e-85
Identity = 159/159 (100.00%), Postives = 159/159 (100.00%), Query Frame = 0


Query: 1   MSDEEHHFESKADAGASKTYPQQAGTIRKNGYIVIKGRPCKVVEVSTSKTGKHGHAKCHF 60
           MSDEEHHFESKADAGASKTYPQQAGTIRKNGYIVIKGRPCKVVEVSTSKTGKHGHAKCHF
Sbjct: 1   MSDEEHHFESKADAGASKTYPQQAGTIRKNGYIVIKGRPCKVVEVSTSKTGKHGHAKCHF 60

Query: 61  VGIDIFTTKKLEDIVPSSHNCDVPHVSRTDYQLIDISEDGFISLLTDSGSTKDDLRLPTD 120
           VGIDIFTTKKLEDIVPSSHNCDVPHVSRTDYQLIDISEDGFISLLTDSGSTKDDLRLPTD
Sbjct: 61  VGIDIFTTKKLEDIVPSSHNCDVPHVSRTDYQLIDISEDGFISLLTDSGSTKDDLRLPTD 120

Query: 121 ESLLSQIKDGFNEGKDLIVTVMSSMGEEQICALKDIGPK 160
           ESLLSQIKDGFNEGKDLIVTVMSSMGEEQICALKDIGPK
Sbjct: 121 ESLLSQIKDGFNEGKDLIVTVMSSMGEEQICALKDIGPK 159

[Close]

BLAST of MS015213 vs. ExPASy TrEMBL
Match: A0A5D3D1G5 (Eukaryotic translation initiation factor 5A OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1932G00070 PE=3 SV=1)

HSP 1 Score: 317.8 bits (813), Expect = 2.6e-83
Identity = 155/159 (97.48%), Postives = 157/159 (98.74%), Query Frame = 0


Query: 1   MSDEEHHFESKADAGASKTYPQQAGTIRKNGYIVIKGRPCKVVEVSTSKTGKHGHAKCHF 60
           MSDEEHHFESKADAGASKTYPQQAGTIRKNGYIVIKGRPCKVVEVSTSKTGKHGHAKCHF
Sbjct: 1   MSDEEHHFESKADAGASKTYPQQAGTIRKNGYIVIKGRPCKVVEVSTSKTGKHGHAKCHF 60

Query: 61  VGIDIFTTKKLEDIVPSSHNCDVPHVSRTDYQLIDISEDGFISLLTDSGSTKDDLRLPTD 120
           VGIDIFT KKLEDIVPSSHNCDVPHVSRTDYQLIDISEDGFISLLTDSGSTKDDLRLPTD
Sbjct: 61  VGIDIFTAKKLEDIVPSSHNCDVPHVSRTDYQLIDISEDGFISLLTDSGSTKDDLRLPTD 120

Query: 121 ESLLSQIKDGFNEGKDLIVTVMSSMGEEQICALKDIGPK 160
           ++LLSQIKDGF EGKDLIVTVMSSMGEEQICALKDIGPK
Sbjct: 121 DNLLSQIKDGFAEGKDLIVTVMSSMGEEQICALKDIGPK 159

[Close]

BLAST of MS015213 vs. ExPASy TrEMBL
Match: A0A1S3BFT8 (Eukaryotic translation initiation factor 5A OS=Cucumis melo OX=3656 GN=LOC103489162 PE=3 SV=1)

HSP 1 Score: 317.8 bits (813), Expect = 2.6e-83
Identity = 155/159 (97.48%), Postives = 157/159 (98.74%), Query Frame = 0


Query: 1   MSDEEHHFESKADAGASKTYPQQAGTIRKNGYIVIKGRPCKVVEVSTSKTGKHGHAKCHF 60
           MSDEEHHFESKADAGASKTYPQQAGTIRKNGYIVIKGRPCKVVEVSTSKTGKHGHAKCHF
Sbjct: 1   MSDEEHHFESKADAGASKTYPQQAGTIRKNGYIVIKGRPCKVVEVSTSKTGKHGHAKCHF 60

Query: 61  VGIDIFTTKKLEDIVPSSHNCDVPHVSRTDYQLIDISEDGFISLLTDSGSTKDDLRLPTD 120
           VGIDIFT KKLEDIVPSSHNCDVPHVSRTDYQLIDISEDGFISLLTDSGSTKDDLRLPTD
Sbjct: 61  VGIDIFTAKKLEDIVPSSHNCDVPHVSRTDYQLIDISEDGFISLLTDSGSTKDDLRLPTD 120

Query: 121 ESLLSQIKDGFNEGKDLIVTVMSSMGEEQICALKDIGPK 160
           ++LLSQIKDGF EGKDLIVTVMSSMGEEQICALKDIGPK
Sbjct: 121 DNLLSQIKDGFAEGKDLIVTVMSSMGEEQICALKDIGPK 159

[Close]

BLAST of MS015213 vs. ExPASy TrEMBL
Match: A0A6J1JGN3 (Eukaryotic translation initiation factor 5A OS=Cucurbita maxima OX=3661 GN=LOC111486732 PE=3 SV=1)

HSP 1 Score: 313.2 bits (801), Expect = 6.4e-82
Identity = 152/159 (95.60%), Postives = 156/159 (98.11%), Query Frame = 0


Query: 1   MSDEEHHFESKADAGASKTYPQQAGTIRKNGYIVIKGRPCKVVEVSTSKTGKHGHAKCHF 60
           MSDEEHHFESKADAGASKTYPQQAGTIRKNGYIVIKGRPCKVVEVSTSKTGKHGHAKCHF
Sbjct: 1   MSDEEHHFESKADAGASKTYPQQAGTIRKNGYIVIKGRPCKVVEVSTSKTGKHGHAKCHF 60

Query: 61  VGIDIFTTKKLEDIVPSSHNCDVPHVSRTDYQLIDISEDGFISLLTDSGSTKDDLRLPTD 120
           V IDIFT+KKLEDIVPSSHNCDVPHVSRTDYQLIDISEDGFISLLTDSG+TKDDLRLP D
Sbjct: 61  VAIDIFTSKKLEDIVPSSHNCDVPHVSRTDYQLIDISEDGFISLLTDSGNTKDDLRLPND 120

Query: 121 ESLLSQIKDGFNEGKDLIVTVMSSMGEEQICALKDIGPK 160
           ESL++QIKDGF EGKDLIVTVMSSMGEEQICALKDIGPK
Sbjct: 121 ESLMTQIKDGFAEGKDLIVTVMSSMGEEQICALKDIGPK 159

[Close]

BLAST of MS015213 vs. ExPASy TrEMBL
Match: A0A0A0KU59 (Eukaryotic translation initiation factor 5A OS=Cucumis sativus OX=3659 GN=Csa_5G606480 PE=3 SV=1)

HSP 1 Score: 313.2 bits (801), Expect = 6.4e-82
Identity = 152/159 (95.60%), Postives = 155/159 (97.48%), Query Frame = 0


Query: 1   MSDEEHHFESKADAGASKTYPQQAGTIRKNGYIVIKGRPCKVVEVSTSKTGKHGHAKCHF 60
           MSDEEHHFESKADAGASKTYPQQAG IRKNGYIVIKGRPCKVVEVSTSKTGKHGHAKCHF
Sbjct: 1   MSDEEHHFESKADAGASKTYPQQAGAIRKNGYIVIKGRPCKVVEVSTSKTGKHGHAKCHF 60

Query: 61  VGIDIFTTKKLEDIVPSSHNCDVPHVSRTDYQLIDISEDGFISLLTDSGSTKDDLRLPTD 120
           VGIDIFT KKLEDIVPSSHNCDVPHVSRTDYQLIDISEDGFISLLTDSG+TKDDLRLPTD
Sbjct: 61  VGIDIFTAKKLEDIVPSSHNCDVPHVSRTDYQLIDISEDGFISLLTDSGNTKDDLRLPTD 120

Query: 121 ESLLSQIKDGFNEGKDLIVTVMSSMGEEQICALKDIGPK 160
           + LL+QIKDGF EGKDLIVTVMSSMGEEQICALKDIGPK
Sbjct: 121 DGLLTQIKDGFAEGKDLIVTVMSSMGEEQICALKDIGPK 159

[Close]

BLAST of MS015213 vs. TAIR 10
Match: AT1G13950.1 (eukaryotic elongation factor 5A-1 )

HSP 1 Score: 287.7 bits (735), Expect = 5.6e-78
Identity = 138/159 (86.79%), Postives = 152/159 (95.60%), Query Frame = 0


Query: 1   MSDEEHHFESKADAGASKTYPQQAGTIRKNGYIVIKGRPCKVVEVSTSKTGKHGHAKCHF 60
           MSDEEHHFES +DAGASKTYPQQAGTIRKNGYIVIK RPCKVVEVSTSKTGKHGHAKCHF
Sbjct: 1   MSDEEHHFES-SDAGASKTYPQQAGTIRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHF 60

Query: 61  VGIDIFTTKKLEDIVPSSHNCDVPHVSRTDYQLIDISEDGFISLLTDSGSTKDDLRLPTD 120
           V IDIFT+KKLEDIVPSSHNCDVPHV+RTDYQLIDISEDG++SLLTD+GSTKDDL+LP D
Sbjct: 61  VAIDIFTSKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGYVSLLTDNGSTKDDLKLPND 120

Query: 121 ESLLSQIKDGFNEGKDLIVTVMSSMGEEQICALKDIGPK 160
           ++LL QIK GF++GKDL+V+VMS+MGEEQI ALKDIGPK
Sbjct: 121 DTLLQQIKSGFDDGKDLVVSVMSAMGEEQINALKDIGPK 158

[Close]

BLAST of MS015213 vs. TAIR 10
Match: AT1G69410.1 (eukaryotic elongation factor 5A-3 )

HSP 1 Score: 286.6 bits (732), Expect = 1.2e-77
Identity = 134/159 (84.28%), Postives = 153/159 (96.23%), Query Frame = 0


Query: 1   MSDEEHHFESKADAGASKTYPQQAGTIRKNGYIVIKGRPCKVVEVSTSKTGKHGHAKCHF 60
           MSD+EHHFES +DAGASKTYPQQAG IRK G+IVIKGRPCKVVEVSTSKTGKHGHAKCHF
Sbjct: 1   MSDDEHHFES-SDAGASKTYPQQAGNIRKGGHIVIKGRPCKVVEVSTSKTGKHGHAKCHF 60

Query: 61  VGIDIFTTKKLEDIVPSSHNCDVPHVSRTDYQLIDISEDGFISLLTDSGSTKDDLRLPTD 120
           V IDIFT+KKLEDIVPSSHNCDVPHV+R DYQLIDISEDGF+SLLTD+GSTKDDL+LPTD
Sbjct: 61  VAIDIFTSKKLEDIVPSSHNCDVPHVNRVDYQLIDISEDGFVSLLTDNGSTKDDLKLPTD 120

Query: 121 ESLLSQIKDGFNEGKDLIVTVMSSMGEEQICALKDIGPK 160
           E+LL+Q+K+GF EGKD++V+VMS+MGEEQ+CALK++GPK
Sbjct: 121 EALLTQLKNGFEEGKDIVVSVMSAMGEEQMCALKEVGPK 158

[Close]

BLAST of MS015213 vs. TAIR 10
Match: AT1G26630.1 (Eukaryotic translation initiation factor 5A-1 (eIF-5A 1) protein )

HSP 1 Score: 266.2 bits (679), Expect = 1.7e-71
Identity = 124/157 (78.98%), Postives = 145/157 (92.36%), Query Frame = 0


Query: 1   MSDEEHHFESKADAGASKTYPQQAGTIRKNGYIVIKGRPCKVVEVSTSKTGKHGHAKCHF 60
           MSD+EHHFE+ +++GASKTYPQ AG IRK G+IVIK RPCKVVEVSTSKTGKHGHAKCHF
Sbjct: 1   MSDDEHHFEA-SESGASKTYPQSAGNIRKGGHIVIKNRPCKVVEVSTSKTGKHGHAKCHF 60

Query: 61  VGIDIFTTKKLEDIVPSSHNCDVPHVSRTDYQLIDISEDGFISLLTDSGSTKDDLRLPTD 120
           V IDIFT KKLEDIVPSSHNCDVPHV+R DYQLIDI+EDGF+SLLTDSG TKDDL+LPTD
Sbjct: 61  VAIDIFTAKKLEDIVPSSHNCDVPHVNRVDYQLIDITEDGFVSLLTDSGGTKDDLKLPTD 120

Query: 121 ESLLSQIKDGFNEGKDLIVTVMSSMGEEQICALKDIG 158
           + L +Q++ GF+EGKD++V+VMSSMGEEQICA+K++G
Sbjct: 121 DGLTAQMRLGFDEGKDIVVSVMSSMGEEQICAVKEVG 156

[Close]

BLAST of MS015213 vs. TAIR 10
Match: AT1G26630.2 (Eukaryotic translation initiation factor 5A-1 (eIF-5A 1) protein )

HSP 1 Score: 219.9 bits (559), Expect = 1.4e-57
Identity = 103/127 (81.10%), Postives = 116/127 (91.34%), Query Frame = 0


Query: 1   MSDEEHHFESKADAGASKTYPQQAGTIRKNGYIVIKGRPCKVVEVSTSKTGKHGHAKCHF 60
           MSD+EHHFE+ +++GASKTYPQ AG IRK G+IVIK RPCKVVEVSTSKTGKHGHAKCHF
Sbjct: 1   MSDDEHHFEA-SESGASKTYPQSAGNIRKGGHIVIKNRPCKVVEVSTSKTGKHGHAKCHF 60

Query: 61  VGIDIFTTKKLEDIVPSSHNCDVPHVSRTDYQLIDISEDGFISLLTDSGSTKDDLRLPTD 120
           V IDIFT KKLEDIVPSSHNCDVPHV+R DYQLIDI+EDGF+SLLTDSG TKDDL+LPTD
Sbjct: 61  VAIDIFTAKKLEDIVPSSHNCDVPHVNRVDYQLIDITEDGFVSLLTDSGGTKDDLKLPTD 120

Query: 121 ESLLSQI 128
           + L +Q+
Sbjct: 121 DGLTAQL 126

The following BLAST results are available for this feature:

BLAST of MS015213 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022151244.1	3.4e-85	100.00	eukaryotic translation initiation factor 5A-4-like [Momordica charantia]	[more]
XP_038893432.1	2.4e-83	98.11	eukaryotic translation initiation factor 5A-4 [Benincasa hispida]	[more]
XP_008446407.1	5.4e-83	97.48	PREDICTED: eukaryotic translation initiation factor 5A-2 [Cucumis melo] >KAA0034...	[more]
XP_022957459.1	1.3e-81	95.60	eukaryotic translation initiation factor 5A-2-like [Cucurbita moschata] >XP_0229...	[more]
KAG7032242.1	1.3e-81	95.60	Eukaryotic translation initiation factor 5A-2 [Cucurbita argyrosperma subsp. arg...	[more]

BLAST of MS015213 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P24922	1.5e-80	89.94	Eukaryotic translation initiation factor 5A-2 OS=Nicotiana plumbaginifolia OX=40...	[more]
Q9AXJ4	1.5e-80	89.31	Eukaryotic translation initiation factor 5A OS=Manihot esculenta OX=3983 PE=2 SV...	[more]
P56336	2.6e-80	91.19	Eukaryotic translation initiation factor 5A-4 OS=Solanum tuberosum OX=4113 GN=EI...	[more]
Q9AXQ4	4.5e-80	91.19	Eukaryotic translation initiation factor 5A-3 OS=Solanum lycopersicum OX=4081 PE...	[more]
Q945F4	5.8e-80	89.31	Eukaryotic translation initiation factor 5A-2 OS=Medicago sativa OX=3879 PE=2 SV...	[more]

BLAST of MS015213 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DCF3	1.6e-85	100.00	Eukaryotic translation initiation factor 5A OS=Momordica charantia OX=3673 GN=LO...	[more]
A0A5D3D1G5	2.6e-83	97.48	Eukaryotic translation initiation factor 5A OS=Cucumis melo var. makuwa OX=11946...	[more]
A0A1S3BFT8	2.6e-83	97.48	Eukaryotic translation initiation factor 5A OS=Cucumis melo OX=3656 GN=LOC103489...	[more]
A0A6J1JGN3	6.4e-82	95.60	Eukaryotic translation initiation factor 5A OS=Cucurbita maxima OX=3661 GN=LOC11...	[more]
A0A0A0KU59	6.4e-82	95.60	Eukaryotic translation initiation factor 5A OS=Cucumis sativus OX=3659 GN=Csa_5G...	[more]

BLAST of MS015213 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G13950.1	5.6e-78	86.79	eukaryotic elongation factor 5A-1	[more]
AT1G69410.1	1.2e-77	84.28	eukaryotic elongation factor 5A-3	[more]
AT1G26630.1	1.7e-71	78.98	Eukaryotic translation initiation factor 5A-1 (eIF-5A 1) protein	[more]
AT1G26630.2	1.4e-57	81.10	Eukaryotic translation initiation factor 5A-1 (eIF-5A 1) protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR020189	Translation elongation factor, IF5A C-terminal	SMART	SM01376	eIF_5a_2	coord: 85..154 e-value: 2.1E-32 score: 123.6
IPR020189	Translation elongation factor, IF5A C-terminal	PFAM	PF01287	eIF-5a	coord: 85..154 e-value: 9.0E-29 score: 99.4
IPR014722	Ribosomal protein L2, domain 2	GENE3D	2.30.30.30		coord: 1..85 e-value: 1.4E-43 score: 148.6
IPR001884	Translation elongation factor IF5A-like	TIGRFAM	TIGR00037	TIGR00037	coord: 17..153 e-value: 6.3E-41 score: 137.2
IPR001884	Translation elongation factor IF5A-like	PIRSF	PIRSF003025	Transl_init_eIF5A	coord: 1..157 e-value: 5.2E-67 score: 219.8
IPR001884	Translation elongation factor IF5A-like	PANTHER	PTHR11673	TRANSLATION INITIATION FACTOR 5A FAMILY MEMBER	coord: 1..156
None	No IPR available	GENE3D	2.40.50.140		coord: 86..158 e-value: 1.5E-33 score: 116.7
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..21
None	No IPR available	PANTHER	PTHR11673:SF42	BNAA07G09420D PROTEIN	coord: 1..156
None	No IPR available	CDD	cd04468	S1_eIF5A	coord: 86..156 e-value: 1.77422E-31 score: 106.082
IPR019769	Translation elongation factor, IF5A, hypusine site	PROSITE	PS00302	IF5A_HYPUSINE	coord: 50..57
IPR008991	Translation protein SH3-like domain superfamily	SUPERFAMILY	50104	Translation proteins SH3-like domain	coord: 15..93
IPR012340	Nucleic acid-binding, OB-fold	SUPERFAMILY	50249	Nucleic acid-binding proteins	coord: 86..156

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS015213.1	MS015213.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0045901	positive regulation of translational elongation
biological_process	GO:0045905	positive regulation of translational termination
molecular_function	GO:0043022	ribosome binding
molecular_function	GO:0003723	RNA binding
molecular_function	GO:0003746	translation elongation factor activity